Carg11517 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11517
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	alpha/beta-Hydrolases superfamily protein
Location	Carg_Chr17: 9461422 .. 9462192 (-)
RNA-Seq Expression	Carg11517
Synteny	Carg11517

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGACCAGGCCTTATCTCCCATCCTTCTCTCATTGATTTCCCCTGCTAATTCAGTCAATCCCCTCCCGAAATCGTCCTCCACCGCCTCCTCCGCCCTCCACGGCCGCCATTGCCAAATCCCCCTCTCTCCGATTGAAAACCCGCCTCCTTGCTTCCTTCTCCTCAGTCTGCTTCGACCTCGCCGCGCTCGCCCCAATGGCACCGTCAACCGTTGCCTCCTACGCCTCTTCGATATCAAATCCGCCGCCAATCCCGTCAAGCCCATCCACGGCGTCGTCTCCTTCGACGTCATTGTCGATTCCTCACGCAACCTCTCCATCCGCGTCTTTACCCCTTCCTCCAACGCTATCTCTCTTCCCATCCTCATCTTCTTTCATGGCGGCGGCTTCTCCTTTCTCAGCAACGCCTCTTTCTCCTACGTCACCCTCTACCGCTGCTTCGCCCGCCGCATACCCGCAGTCGTCCTCTCCGTCAACTACCGCCTCTCTCCCGAACACCGCTTCCCTTCCCAATACGACGACGGCTTCGACGTCCTCCAATTCCTCGACCACGAAAACAACACCATCGGCCTCCTCCCTCCAACCGCCGACCTCTCCAAATGTTTCCTCGCCGGTGACAGCGCCGGCGCCAATCTCGCTCACCACGTAGCCGTCCGATTCTCCCGACAAAAATCACAATTCCAAAGAGCCAACATCATCGGATTAGTATCGATTCAACCGTTCTTCGGCGGACAGGATCGGATCGAGACCACTCTGCTTCGAACGTGA

mRNA sequence

ATGGCGGACCAGGCCTTATCTCCCATCCTTCTCTCATTGATTTCCCCTGCTAATTCAGTCAATCCCCTCCCGAAATCGTCCTCCACCGCCTCCTCCGCCCTCCACGGCCGCCATTGCCAAATCCCCCTCTCTCCGATTGAAAACCCGCCTCCTTGCTTCCTTCTCCTCAGTCTGCTTCGACCTCGCCGCGCTCGCCCCAATGGCACCGTCAACCGTTGCCTCCTACGCCTCTTCGATATCAAATCCGCCGCCAATCCCGTCAAGCCCATCCACGGCGTCGTCTCCTTCGACGTCATTGTCGATTCCTCACGCAACCTCTCCATCCGCGTCTTTACCCCTTCCTCCAACGCTATCTCTCTTCCCATCCTCATCTTCTTTCATGGCGGCGGCTTCTCCTTTCTCAGCAACGCCTCTTTCTCCTACGTCACCCTCTACCGCTGCTTCGCCCGCCGCATACCCGCAGTCGTCCTCTCCGTCAACTACCGCCTCTCTCCCGAACACCGCTTCCCTTCCCAATACGACGACGGCTTCGACGTCCTCCAATTCCTCGACCACGAAAACAACACCATCGGCCTCCTCCCTCCAACCGCCGACCTCTCCAAATGTTTCCTCGCCGGTGACAGCGCCGGCGCCAATCTCGCTCACCACGTAGCCGTCCGATTCTCCCGACAAAAATCACAATTCCAAAGAGCCAACATCATCGGATTAGTATCGATTCAACCGTTCTTCGGCGGACAGGATCGGATCGAGACCACTCTGCTTCGAACGTGA

Coding sequence (CDS)

ATGGCGGACCAGGCCTTATCTCCCATCCTTCTCTCATTGATTTCCCCTGCTAATTCAGTCAATCCCCTCCCGAAATCGTCCTCCACCGCCTCCTCCGCCCTCCACGGCCGCCATTGCCAAATCCCCCTCTCTCCGATTGAAAACCCGCCTCCTTGCTTCCTTCTCCTCAGTCTGCTTCGACCTCGCCGCGCTCGCCCCAATGGCACCGTCAACCGTTGCCTCCTACGCCTCTTCGATATCAAATCCGCCGCCAATCCCGTCAAGCCCATCCACGGCGTCGTCTCCTTCGACGTCATTGTCGATTCCTCACGCAACCTCTCCATCCGCGTCTTTACCCCTTCCTCCAACGCTATCTCTCTTCCCATCCTCATCTTCTTTCATGGCGGCGGCTTCTCCTTTCTCAGCAACGCCTCTTTCTCCTACGTCACCCTCTACCGCTGCTTCGCCCGCCGCATACCCGCAGTCGTCCTCTCCGTCAACTACCGCCTCTCTCCCGAACACCGCTTCCCTTCCCAATACGACGACGGCTTCGACGTCCTCCAATTCCTCGACCACGAAAACAACACCATCGGCCTCCTCCCTCCAACCGCCGACCTCTCCAAATGTTTCCTCGCCGGTGACAGCGCCGGCGCCAATCTCGCTCACCACGTAGCCGTCCGATTCTCCCGACAAAAATCACAATTCCAAAGAGCCAACATCATCGGATTAGTATCGATTCAACCGTTCTTCGGCGGACAGGATCGGATCGAGACCACTCTGCTTCGAACGTGA

Protein sequence

MADQALSPILLSLISPANSVNPLPKSSSTASSALHGRHCQIPLSPIENPPPCFLLLSLLRPRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFGGQDRIETTLLRT

Homology

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BLAST of Carg11517 vs. NCBI nr
Match: KAG7014576.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 508.8 bits (1309), Expect = 2.7e-140
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MADQALSPILLSLISPANSVNPLPKSSSTASSALHGRHCQIPLSPIENPPPCFLLLSLLR 60
           MADQALSPILLSLISPANSVNPLPKSSSTASSALHGRHCQIPLSPIENPPPCFLLLSLLR
Sbjct: 1   MADQALSPILLSLISPANSVNPLPKSSSTASSALHGRHCQIPLSPIENPPPCFLLLSLLR 60

Query: 61  PRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISL 120
           PRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISL
Sbjct: 61  PRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISL 120

Query: 121 PILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVL 180
           PILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVL
Sbjct: 121 PILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVL 180

Query: 181 QFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQ 240
           QFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQ
Sbjct: 181 QFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQ 240

Query: 241 PFFGGQDRIETTLLRT 257
           PFFGGQDRIETTLLRT
Sbjct: 241 PFFGGQDRIETTLLRT 256

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BLAST of Carg11517 vs. NCBI nr
Match: KAG6576059.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487.6 bits (1254), Expect = 6.4e-134
Identity = 245/253 (96.84%), Postives = 249/253 (98.42%), Query Frame = 0


Query: 1   MADQALSPILLSLISPANSVNPLPKSSSTASSALHGRHCQIPLSPIENPPPCFLLLSLLR 60
           MADQALSPILLSLISPANSVNPLPKSSSTASSALHGRHCQIPLSPIENPPPCFLLLSLLR
Sbjct: 1   MADQALSPILLSLISPANSVNPLPKSSSTASSALHGRHCQIPLSPIENPPPCFLLLSLLR 60

Query: 61  PRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISL 120
           PRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISL
Sbjct: 61  PRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISL 120

Query: 121 PILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVL 180
           PILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYD GFDVL
Sbjct: 121 PILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDYGFDVL 180

Query: 181 QFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQ 240
           QFLDHENNTIGLL PTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQ
Sbjct: 181 QFLDHENNTIGLLAPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQ 240

Query: 241 PFFGGQDRIETTL 254
           PFFGGQ+R ++ +
Sbjct: 241 PFFGGQERTQSEI 253

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BLAST of Carg11517 vs. NCBI nr
Match: XP_038896610.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 1.3e-86
Identity = 176/249 (70.68%), Postives = 200/249 (80.32%), Query Frame = 0


Query: 11  LSLISPANSVNPL--PKSSSTASSALHGRHCQIPLSPIENPPPCFLLL----SLLRPRRA 70
           LS IS + S+NP+  P  SS  SS         P+S   + P    LL    S+      
Sbjct: 38  LSFISSSLSLNPMDPPPPSSPPSS-------PPPISKSPSLPWRTRLLVCISSICGDLAT 97

Query: 71  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 130
           RPNGT+NR L RLFD+KS ANPVKPIHGV+SFDVIVDSSRNLS+RVFTPSS+A SLP+LI
Sbjct: 98  RPNGTINRRLFRLFDVKSPANPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAASLPVLI 157

Query: 131 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 190
           FFHGGGF+FLSN+SFSY  + R  ARRIPAVVLSV+YRLSPEHRFPSQYDDGFDVL+FLD
Sbjct: 158 FFHGGGFAFLSNSSFSYAAVCRRLARRIPAVVLSVDYRLSPEHRFPSQYDDGFDVLRFLD 217

Query: 191 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 250
           HENNT+GLLPP+ADLSKCFLAGDSAGANLAHHVAVRF RQ+SQFQRA IIGLVSIQPFFG
Sbjct: 218 HENNTVGLLPPSADLSKCFLAGDSAGANLAHHVAVRFCRQRSQFQRAKIIGLVSIQPFFG 277

Query: 251 GQDRIETTL 254
           G++R E+ +
Sbjct: 278 GEERTESEI 279

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BLAST of Carg11517 vs. NCBI nr
Match: XP_004148971.1 (probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 321.6 bits (823), Expect = 6.0e-84
Identity = 154/189 (81.48%), Postives = 172/189 (91.01%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 124
           RPNGTVNR L RLFD KS  NPVKPIHGV+SFDVIVDSSRNLS+RVFTPSS+  SLPILI
Sbjct: 39  RPNGTVNRRLFRLFDFKSPPNPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDVASLPILI 98

Query: 125 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 184
           FFHGGGF+ LSN+SFSYV + R FARR+PA+VLSV+YRLSPEHRFPSQYDDGFDVL+FLD
Sbjct: 99  FFHGGGFALLSNSSFSYVAVCRRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLD 158

Query: 185 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 244
           HE+NTIGLLPP ADLSKCFLAGDSAGANLAHHVAVRF RQ+SQF+RA ++GLVSIQPFFG
Sbjct: 159 HESNTIGLLPPNADLSKCFLAGDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFG 218

Query: 245 GQDRIETTL 254
           G++R E  +
Sbjct: 219 GEERTEAEI 227

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BLAST of Carg11517 vs. NCBI nr
Match: XP_008451245.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 321.2 bits (822), Expect = 7.9e-84
Identity = 154/189 (81.48%), Postives = 172/189 (91.01%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 124
           RPNGTVNR L RLFD KS  NPVKPIHGV+SFDVIVDSSRNLS+RVFTPSS+A SLPILI
Sbjct: 41  RPNGTVNRRLFRLFDFKSPPNPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAASLPILI 100

Query: 125 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 184
           FFHGGGF+ LSN+SFSYV + R FARR+PA+VLSV+YRLSPEHRFPSQYDDGFDVL+FLD
Sbjct: 101 FFHGGGFALLSNSSFSYVAVCRRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLD 160

Query: 185 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 244
           HE+NTIGLLPP ADLS CFLAGDSAGANLAHHVAVRF RQ+SQF+RA ++GLVSIQPFFG
Sbjct: 161 HESNTIGLLPPNADLSNCFLAGDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFG 220

Query: 245 GQDRIETTL 254
           G++R E  +
Sbjct: 221 GEERTEAEI 229

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BLAST of Carg11517 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 8.0e-55
Identity = 101/197 (51.27%), Postives = 146/197 (74.11%), Query Frame = 0


Query: 55  LLSLLRPRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPS 114
           ++S +     RP+GT+NR  LRLFD ++  NP KP++ V + D +VD SR+L  R++TP 
Sbjct: 23  VISTMTDNAQRPDGTINRRFLRLFDFRAPPNP-KPVNIVSTSDFVVDQSRDLWFRLYTPH 82

Query: 115 SNAISLPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYD 174
            +   +P+++FFHGGGF+FLS  ++ Y  + R FAR++PA V+SVNYRL+PEHR+P+QYD
Sbjct: 83  VSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYD 142

Query: 175 DGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQ-KSQFQRANI 234
           DGFD L++++  + +I  LP  ADLS+CF AGDSAG N+AH+VA+R  R+ +S F    +
Sbjct: 143 DGFDALKYIEENHGSI--LPANADLSRCFFAGDSAGGNIAHNVAIRICREPRSSFTAVKL 202

Query: 235 IGLVSIQPFFGGQDRIE 251
           IGL+SIQPFFGG++R E
Sbjct: 203 IGLISIQPFFGGEERTE 216

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BLAST of Carg11517 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.3e-30
Identity = 84/212 (39.62%), Postives = 118/212 (55.66%), Query Frame = 0


Query: 54  LLLSLLRPRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTP 113
           L  +LLR    RP+GT NR L    D K  AN   P++GV SFDVI+D   NL  RV+ P
Sbjct: 29  LAYNLLR----RPDGTFNRHLAEFLDRKVPAN-ANPVNGVFSFDVIIDRQTNLLSRVYRP 88

Query: 114 SSNAIS--------------LPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSV 173
           +    S              +P+++FFHGG F+  S  S  Y TL R       AVV+SV
Sbjct: 89  ADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSV 148

Query: 174 NYRLSPEHRFPSQYDDGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAV 233
           NYR +PE+R+P  YDDG+ VL+++   N++  L        + FLAGDS+G N+ H+VAV
Sbjct: 149 NYRRAPENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAV 208

Query: 234 RFSRQKSQFQRANIIGLVSIQPFFGGQDRIET 252
           R     +   R +++G + + P FGG +R E+
Sbjct: 209 R-----AVESRIDVLGNILLNPMFGGTERTES 227

[Close]

BLAST of Carg11517 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 7.8e-26
Identity = 76/204 (37.25%), Postives = 108/204 (52.94%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPS---------- 124
           RP+G+ NR L    D K  AN   P+ GV SFD  VDS+ NL  R++ P+          
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSF-PLDGVFSFD-HVDSTTNLLTRIYQPASLLHQTRHGT 95

Query: 125 -------SNAISLPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEH 184
                  S    +P+LIFFHGG F+  S  S  Y T  R        VV+SV+YR SPEH
Sbjct: 96  LELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEH 155

Query: 185 RFPSQYDDGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQ 244
           R+P  YDDG++ L ++    + + L          +LAGDS+G N+AH+VAVR +     
Sbjct: 156 RYPCAYDDGWNALNWV---KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---- 215

Query: 245 FQRANIIGLVSIQPFFGGQDRIET 252
            +   ++G + + P FGGQ+R ++
Sbjct: 216 -EGVKVLGNILLHPMFGGQERTQS 229

[Close]

BLAST of Carg11517 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.3e-25
Identity = 74/203 (36.45%), Postives = 105/203 (51.72%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPS---------- 124
           RP+GT NR L    D K  AN   P+ GV SFDV++D   NL  RV+ P+          
Sbjct: 36  RPDGTFNRHLAEYLDRKVTAN-ANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSI 95

Query: 125 ------SNAISLPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHR 184
                  +   +P+++FFHGG F+  S  S  Y TL R        VV+SVNYR +PE+ 
Sbjct: 96  LDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENP 155

Query: 185 FPSQYDDGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQF 244
           +P  YDDG+  L ++   N+   L          FLAGDS+G N+AH+VA+R        
Sbjct: 156 YPCAYDDGWIALNWV---NSRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE----- 215

Query: 245 QRANIIGLVSIQPFFGGQDRIET 252
              +++G + + P FGG +R E+
Sbjct: 216 SGIDVLGNILLNPMFGGNERTES 229

[Close]

BLAST of Carg11517 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.3e-25
Identity = 67/192 (34.90%), Postives = 102/192 (53.12%), Query Frame = 0


Query: 67  NGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAI-----SLP 126
           NG + R +   F   S    + P +GVVS D +    +NLS+R++ P ++        +P
Sbjct: 18  NGGIERLVPETFVPPS----LNPENGVVSKDAVYSPEKNLSLRIYLPQNSVYETGEKKIP 77

Query: 127 ILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQ 186
           +L++FHGGGF   +  S  Y T           + +SV YR +PEH  P+ Y+D +D +Q
Sbjct: 78  LLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTLYEDSWDAIQ 137

Query: 187 FLDHENNTIG---LLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVS 246
           ++       G    L   AD SK FLAGDSAGAN+AHH+A+R  ++K   +   I G++ 
Sbjct: 138 WIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPENFKISGMIL 197

Query: 247 IQPFFGGQDRIE 251
             P+F  +  IE
Sbjct: 198 FHPYFLSKALIE 205

[Close]

BLAST of Carg11517 vs. ExPASy TrEMBL
Match: A0A0A0KA95 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375730 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.9e-84
Identity = 154/189 (81.48%), Postives = 172/189 (91.01%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 124
           RPNGTVNR L RLFD KS  NPVKPIHGV+SFDVIVDSSRNLS+RVFTPSS+  SLPILI
Sbjct: 39  RPNGTVNRRLFRLFDFKSPPNPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDVASLPILI 98

Query: 125 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 184
           FFHGGGF+ LSN+SFSYV + R FARR+PA+VLSV+YRLSPEHRFPSQYDDGFDVL+FLD
Sbjct: 99  FFHGGGFALLSNSSFSYVAVCRRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLD 158

Query: 185 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 244
           HE+NTIGLLPP ADLSKCFLAGDSAGANLAHHVAVRF RQ+SQF+RA ++GLVSIQPFFG
Sbjct: 159 HESNTIGLLPPNADLSKCFLAGDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFG 218

Query: 245 GQDRIETTL 254
           G++R E  +
Sbjct: 219 GEERTEAEI 227

[Close]

BLAST of Carg11517 vs. ExPASy TrEMBL
Match: A0A5A7UUP4 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold54G00630 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.8e-84
Identity = 154/189 (81.48%), Postives = 172/189 (91.01%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 124
           RPNGTVNR L RLFD KS  NPVKPIHGV+SFDVIVDSSRNLS+RVFTPSS+A SLPILI
Sbjct: 40  RPNGTVNRRLFRLFDFKSPPNPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAASLPILI 99

Query: 125 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 184
           FFHGGGF+ LSN+SFSYV + R FARR+PA+VLSV+YRLSPEHRFPSQYDDGFDVL+FLD
Sbjct: 100 FFHGGGFALLSNSSFSYVAVCRRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLD 159

Query: 185 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 244
           HE+NTIGLLPP ADLS CFLAGDSAGANLAHHVAVRF RQ+SQF+RA ++GLVSIQPFFG
Sbjct: 160 HESNTIGLLPPNADLSNCFLAGDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFG 219

Query: 245 GQDRIETTL 254
           G++R E  +
Sbjct: 220 GEERTEAEI 228

[Close]

BLAST of Carg11517 vs. ExPASy TrEMBL
Match: A0A1S3BR05 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103492592 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.8e-84
Identity = 154/189 (81.48%), Postives = 172/189 (91.01%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 124
           RPNGTVNR L RLFD KS  NPVKPIHGV+SFDVIVDSSRNLS+RVFTPSS+A SLPILI
Sbjct: 41  RPNGTVNRRLFRLFDFKSPPNPVKPIHGVLSFDVIVDSSRNLSVRVFTPSSDAASLPILI 100

Query: 125 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 184
           FFHGGGF+ LSN+SFSYV + R FARR+PA+VLSV+YRLSPEHRFPSQYDDGFDVL+FLD
Sbjct: 101 FFHGGGFALLSNSSFSYVAVCRRFARRLPAIVLSVDYRLSPEHRFPSQYDDGFDVLRFLD 160

Query: 185 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 244
           HE+NTIGLLPP ADLS CFLAGDSAGANLAHHVAVRF RQ+SQF+RA ++GLVSIQPFFG
Sbjct: 161 HESNTIGLLPPNADLSNCFLAGDSAGANLAHHVAVRFCRQRSQFERARVVGLVSIQPFFG 220

Query: 245 GQDRIETTL 254
           G++R E  +
Sbjct: 221 GEERTEAEI 229

[Close]

BLAST of Carg11517 vs. ExPASy TrEMBL
Match: A0A6J1H8J6 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111460629 PE=3 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 2.1e-82
Identity = 154/187 (82.35%), Postives = 168/187 (89.84%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 124
           R NGT+NR L R FD KSA NPVKPIHGV+SFDVIVDSSRNLS+RVFTP S+A SLPIL+
Sbjct: 34  RRNGTLNRRLFRFFDFKSAPNPVKPIHGVLSFDVIVDSSRNLSVRVFTPRSDAPSLPILV 93

Query: 125 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 184
           FFHGGGF+FLSNASFSY  + R  ARRIPAVVLSV+YRLSPEHRFP QYDDGFDVL+FLD
Sbjct: 94  FFHGGGFAFLSNASFSYAAVCRRLARRIPAVVLSVDYRLSPEHRFPCQYDDGFDVLRFLD 153

Query: 185 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 244
           H NNTIGLLPP ADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQ+  IIGL+SIQPFFG
Sbjct: 154 HGNNTIGLLPPNADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQKVKIIGLISIQPFFG 213

Query: 245 GQDRIET 252
           G +R+E+
Sbjct: 214 GVERMES 220

[Close]

BLAST of Carg11517 vs. ExPASy TrEMBL
Match: A0A6J1L0P0 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111498022 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 6.1e-82
Identity = 154/187 (82.35%), Postives = 168/187 (89.84%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAISLPILI 124
           R NGT+NR L RLFD KS+ NPVKPIHGV+SFDVIVDSSRNLS+RVFTP S+A +LPILI
Sbjct: 33  RRNGTLNRRLFRLFDFKSSPNPVKPIHGVLSFDVIVDSSRNLSVRVFTPRSDAPTLPILI 92

Query: 125 FFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQFLD 184
           FFHGGGF+FLSNASFSY  + R  ARRIPAVVLSV+YRLSPEHRFP QYDDGFDVL+FLD
Sbjct: 93  FFHGGGFAFLSNASFSYAAVCRRLARRIPAVVLSVDYRLSPEHRFPCQYDDGFDVLRFLD 152

Query: 185 HENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVSIQPFFG 244
           H NNTIGLLPP ADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQ+  IIGL+SIQPFFG
Sbjct: 153 HGNNTIGLLPPNADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQKVKIIGLISIQPFFG 212

Query: 245 GQDRIET 252
           G  R+E+
Sbjct: 213 GVKRMES 219

[Close]

BLAST of Carg11517 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 215.3 bits (547), Expect = 5.7e-56
Identity = 101/197 (51.27%), Postives = 146/197 (74.11%), Query Frame = 0


Query: 55  LLSLLRPRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPS 114
           ++S +     RP+GT+NR  LRLFD ++  NP KP++ V + D +VD SR+L  R++TP 
Sbjct: 23  VISTMTDNAQRPDGTINRRFLRLFDFRAPPNP-KPVNIVSTSDFVVDQSRDLWFRLYTPH 82

Query: 115 SNAISLPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYD 174
            +   +P+++FFHGGGF+FLS  ++ Y  + R FAR++PA V+SVNYRL+PEHR+P+QYD
Sbjct: 83  VSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYD 142

Query: 175 DGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQ-KSQFQRANI 234
           DGFD L++++  + +I  LP  ADLS+CF AGDSAG N+AH+VA+R  R+ +S F    +
Sbjct: 143 DGFDALKYIEENHGSI--LPANADLSRCFFAGDSAGGNIAHNVAIRICREPRSSFTAVKL 202

Query: 235 IGLVSIQPFFGGQDRIE 251
           IGL+SIQPFFGG++R E
Sbjct: 203 IGLISIQPFFGGEERTE 216

[Close]

BLAST of Carg11517 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 134.0 bits (336), Expect = 1.7e-31
Identity = 84/212 (39.62%), Postives = 118/212 (55.66%), Query Frame = 0


Query: 54  LLLSLLRPRRARPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTP 113
           L  +LLR    RP+GT NR L    D K  AN   P++GV SFDVI+D   NL  RV+ P
Sbjct: 29  LAYNLLR----RPDGTFNRHLAEFLDRKVPAN-ANPVNGVFSFDVIIDRQTNLLSRVYRP 88

Query: 114 SSNAIS--------------LPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSV 173
           +    S              +P+++FFHGG F+  S  S  Y TL R       AVV+SV
Sbjct: 89  ADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSV 148

Query: 174 NYRLSPEHRFPSQYDDGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAV 233
           NYR +PE+R+P  YDDG+ VL+++   N++  L        + FLAGDS+G N+ H+VAV
Sbjct: 149 NYRRAPENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAV 208

Query: 234 RFSRQKSQFQRANIIGLVSIQPFFGGQDRIET 252
           R     +   R +++G + + P FGG +R E+
Sbjct: 209 R-----AVESRIDVLGNILLNPMFGGTERTES 227

[Close]

BLAST of Carg11517 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 5.5e-27
Identity = 76/204 (37.25%), Postives = 108/204 (52.94%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPS---------- 124
           RP+G+ NR L    D K  AN   P+ GV SFD  VDS+ NL  R++ P+          
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSF-PLDGVFSFD-HVDSTTNLLTRIYQPASLLHQTRHGT 95

Query: 125 -------SNAISLPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEH 184
                  S    +P+LIFFHGG F+  S  S  Y T  R        VV+SV+YR SPEH
Sbjct: 96  LELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEH 155

Query: 185 RFPSQYDDGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQ 244
           R+P  YDDG++ L ++    + + L          +LAGDS+G N+AH+VAVR +     
Sbjct: 156 RYPCAYDDGWNALNWV---KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---- 215

Query: 245 FQRANIIGLVSIQPFFGGQDRIET 252
            +   ++G + + P FGGQ+R ++
Sbjct: 216 -EGVKVLGNILLHPMFGGQERTQS 229

[Close]

BLAST of Carg11517 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 9.4e-27
Identity = 74/203 (36.45%), Postives = 105/203 (51.72%), Query Frame = 0


Query: 65  RPNGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPS---------- 124
           RP+GT NR L    D K  AN   P+ GV SFDV++D   NL  RV+ P+          
Sbjct: 36  RPDGTFNRHLAEYLDRKVTAN-ANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSI 95

Query: 125 ------SNAISLPILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHR 184
                  +   +P+++FFHGG F+  S  S  Y TL R        VV+SVNYR +PE+ 
Sbjct: 96  LDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENP 155

Query: 185 FPSQYDDGFDVLQFLDHENNTIGLLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQF 244
           +P  YDDG+  L ++   N+   L          FLAGDS+G N+AH+VA+R        
Sbjct: 156 YPCAYDDGWIALNWV---NSRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE----- 215

Query: 245 QRANIIGLVSIQPFFGGQDRIET 252
              +++G + + P FGG +R E+
Sbjct: 216 SGIDVLGNILLNPMFGGNERTES 229

[Close]

BLAST of Carg11517 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.6e-26
Identity = 67/192 (34.90%), Postives = 102/192 (53.12%), Query Frame = 0


Query: 67  NGTVNRCLLRLFDIKSAANPVKPIHGVVSFDVIVDSSRNLSIRVFTPSSNAI-----SLP 126
           NG + R +   F   S    + P +GVVS D +    +NLS+R++ P ++        +P
Sbjct: 18  NGGIERLVPETFVPPS----LNPENGVVSKDAVYSPEKNLSLRIYLPQNSVYETGEKKIP 77

Query: 127 ILIFFHGGGFSFLSNASFSYVTLYRCFARRIPAVVLSVNYRLSPEHRFPSQYDDGFDVLQ 186
           +L++FHGGGF   +  S  Y T           + +SV YR +PEH  P+ Y+D +D +Q
Sbjct: 78  LLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPTLYEDSWDAIQ 137

Query: 187 FLDHENNTIG---LLPPTADLSKCFLAGDSAGANLAHHVAVRFSRQKSQFQRANIIGLVS 246
           ++       G    L   AD SK FLAGDSAGAN+AHH+A+R  ++K   +   I G++ 
Sbjct: 138 WIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPPENFKISGMIL 197

Query: 247 IQPFFGGQDRIE 251
             P+F  +  IE
Sbjct: 198 FHPYFLSKALIE 205

The following BLAST results are available for this feature:

BLAST of Carg11517 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014576.1	2.7e-140	100.00	putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6576059.1	6.4e-134	96.84	putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038896610.1	1.3e-86	70.68	probable carboxylesterase 18 [Benincasa hispida]	[more]
XP_004148971.1	6.0e-84	81.48	probable carboxylesterase 18 [Cucumis sativus]	[more]
XP_008451245.1	7.9e-84	81.48	PREDICTED: probable carboxylesterase 18 [Cucumis melo]	[more]

BLAST of Carg11517 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT10	8.0e-55	51.27	Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1	[more]
Q940G6	2.3e-30	39.62	Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1	[more]
Q9LYC1	7.8e-26	37.25	Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1	[more]
Q9MAA7	1.3e-25	36.45	Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1	[more]
Q9LMA7	2.3e-25	34.90	Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1	[more]

BLAST of Carg11517 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KA95	2.9e-84	81.48	Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3757...	[more]
A0A5A7UUP4	3.8e-84	81.48	Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A1S3BR05	3.8e-84	81.48	probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103492592 PE=3 SV=1	[more]
A0A6J1H8J6	2.1e-82	82.35	probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111460629 PE=3 ...	[more]
A0A6J1L0P0	6.1e-82	82.35	probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111498022 PE=3 SV...	[more]

BLAST of Carg11517 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23530.1	5.7e-56	51.27	carboxyesterase 18	[more]
AT5G27320.1	1.7e-31	39.62	alpha/beta-Hydrolases superfamily protein	[more]
AT3G63010.1	5.5e-27	37.25	alpha/beta-Hydrolases superfamily protein	[more]
AT3G05120.1	9.4e-27	36.45	alpha/beta-Hydrolases superfamily protein	[more]
AT1G19190.1	1.6e-26	34.90	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 123..245 e-value: 4.3E-30 score: 105.2
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 52..254 e-value: 1.2E-46 score: 161.6
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 77..247
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 55..251
None	No IPR available	PANTHER	PTHR23024:SF434	ACETYL ESTERASE	coord: 55..251
IPR002168	Lipase, GDXG, putative histidine active site	PROSITE	PS01173	LIPASE_GDXG_HIS	coord: 123..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11517-RA	Carg11517-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009860	pollen tube growth
molecular_function	GO:0052689	carboxylic ester hydrolase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0016787	hydrolase activity