Carg11499 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11499
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	mitochondrial inner membrane protease subunit 2-like
Location	Carg_Chr17: 9357887 .. 9361345 (-)
RNA-Seq Expression	Carg11499
Synteny	Carg11499

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACCTGCAAATTATCTTCTTGAAATGGCTTCCCTCTCCACCTGGTTCCGCTACATCGCCCACAAGCTCGAGTACTCCGTCTCTCTCAGTTGGAAGGTCTTTCACTTTCTCTCTCTAACCCTATTCTTCAATTTTTTTATTTTTTATTTTTTTATGGTTTTTGTTGAATTACTGTGTTTCGGCTTCATAAGTTGTTAGTTTTTGTTGATTATTCGAATATTGTTGGAACTTCTTTAGAATAATCACATTTCTTGCACTGTTTGTTTGTCATCGGGCTTTGGAATTTTCGTTTTTGAAGTTTTAGATATGCATTCTTGGAATTTCAATTCGCATTTCTTGATTTTGTTGTTTTGGTTTTCGATCACTAATGAATCTTCTGGTTTTGTTCATTGTTTATGAGTTATTGACAGGCGATTTCTGTTATAATTGATGTATATCTTTACTAAAAAATATTTAAGTTGATTGGTGCTGTTTGTTTACTGAAGAATTAGTAGAATGCTGTTTGTTTAACTGTTTCTTGAATGGCCACTTCTTCTATGTTAATTCTTGGTTTGTCGGTTTGATTTATGATTAACTCATGTTGTCATTTACCATTTACCTGTCGGGGGCGGGCAAATTAGCAAGGGCATTTAAAAACAAAAGATTAAAGCCCTATATGAATTTAAAATACTCATAGGTTAAATTCTTTCACATGATATCTAAAGAATGTTTAATTTTTTGTTAGTTGCAACTTTCTCATGTTTTCCGTCTCTCCATGTTGGTGTTCATTTCATAGGCTATATATATTCACTGCCCTTTCTTGAGGGAGGTTTGGGAGGAAGTTTTAATTGTGTTTTTGGCTAGATTTTGAATATTTATCGAGGGGCATCATCTCTATATTGCTTGTAACGGTCCAGATCCACCTCTAGCAGATATTGTCTTCTTTGGGCTTTCCCTTTTGGGCTTCCCCTCAAGGCTTTAAAATGCGTCTGCTAGGGGAAGGTTTCCACACCCTTATAAATGGTGATTTGTTCTCCTCCCCAACCAATGTGGGACATCACATTGCTGCTAGGCTCTTTTTCAGAAGAAAACATAATTTATCTATTTCTTTTTAATAAAATTACTGCTTGAAAAATTAGATGAGTGTTTCAAATGAAAAGAATAATTCTTCCGAGTTTTGTCCATATACTTGGTTTCTTGGCCTTCTGTTGTAGTCCTCTATATATATGTTTTTCTTTAATTAATCTCCTTTTTTAAAAAAATTATTTTTCTTTTAATTGTTTCTTTTTCTGAATGAGTGTTCTTTTTAGCTTCTTATAGAGATATAGTTCTATTGTTTGATAAGTTTAAGCTGGCCAGGAATTTCTATGTTGAATGTGTGTAAGCTGCTGGGACTTGACAATTAAACCGTCTGCCAGGAAAAGCAAATGGTGCATAGCTAATGCCAGTTCCCAAAAATTCATATCGTACTTGCTGGACCGTCTTAGATCTTCTGATTTTTTTTTTTCTCTATTCTTATAAGAAATATTAAAATGGTTTATCTCTCCACTCCTTCATGCATCTTCTATCTCTTCTGGATTTTCTTGTAGTTATATCTCGGTTAAATTCTTGTTGGTTATTAGATAGGAAACTTTTGAACTCTAAGAAGTGCTTGATTGAGAAAAAAGTTGAAATGTCTTAACCATTTCAACCTTTCAACTATTGTATATAAACGATTACAAGAAGAATCTTTACTCTGATGCAGAACTATAAAGGAGGTCAAATTACTGACAAAGAAGTAGGTGATGCCGTTTGGAAGAATTTACTTCAGGGAAAGCTCACATACCTCCATTGGATTAAGGGAGAGGAGATGGCCCCAACTGTAGGTGAGGCAGGGGGAACGCTTCTCATACGCAAATTACCTGCTGCAGATCCAACGTAAACTTCGTCTTCCTCTTAGTATAAAAATATATATTCTCTTTTGCTGCAAGAACAGTTTCCTATTCTAGAGAGAACTGATTATGCTTTTCGGAGTAGAAATTGGAATTTAGTCATTGAATAGTTCATATGCTCCTTATAACCTGTAATTTAGGTAGGATCCTACTTCAATTCTAGATTTTCTTAGCTTGTACTTTTTACCAACTCTCTTCCTTAATGGCAGAGATATTTGTTTCACAGGAGTGTTTTTGTTGGGGATGTCGTGGTGGTGAAGGAGCCAGAAAAACCAGAAAACTATCTTGTGAGAAGATTGGTTGCAGTTGAAGGGTATGAAATGCTGTCTACAGATGAAAAAGATAAGCCTTTCATGCTTGAGAAGGATCAGTGCTGGTTGTTGGCAGATAACGAGGAGTTGAAGCCGAAGGTATAATACAATGACAATCACCATAATTTTGCTGTAAATAGTTTTTTCCTCAGCATTGGTATTGGTCTCTCTATGATGGTGGTACCTTTTGGCCCTAGGTCCATACCCTTTTGGTTATCTTGCCATTTGAGATTTCTGGTGCACTTTGAGTTTTATTTTTTATTTATATTTTTTTAATTATGGAATTTTATGAGCAGCTAATGCCAAAACCACTTCATGCTATAAAAACTTTGCTCGTTTTGTTAATTTTCAATCAAGTAATTTTAGCCCCTTTTTGTTGTGTCCTTTCACATAATTGCCGAAAACTATTTGGAGTTCTTTTTTTTTTTTTTTTTTTTTTTTTGTGTGTGTGTGTGTGTAAACGTCCAAACATTCCATTTTAGTTTATGAACTTTGCATAAAAAAACCACTCGTCATTTTGAATTTATAGAAATCTTGATCATACACTTGGAAAAAGTTTTGGACCATGTTCTATGGAAAAACTGTTCTTTTTTTTTTTAAAAGAAGAAACGTACAAAACTTAGTTTTGAACTGCAGTTTCTAGGTGCCAGAAATATAGGAATGCATAAATGCAAATGCATCACATAAGTGGTTTAGGAAGGAAAAAATTGGATTGCTCTATCTCTTGTTTGTTGTTTCCCAACCAGAAGGCTTATTTGTTAAAAGACAAATTCGTCAAAATTTGGTTCAAATTGAACATGCATTCTATGGTTCTCTTTGCTGTGTTGTGTAATGATTTGATAAGTGTTGTAATCTTCATCACAAAACTAAATTTCCCCCCTTCCCTCTCATTTTTAAGGAGGCGTACGACAGTCGAACATTTGGTCCAGTTACGATGTCAGACATTGTAGGCAGAGCCATATATTGCCTACGAACAGCGGTCGACCATGGCCCAGTGCAGAATAGGTTAGCTACTATTCACTGTGTAGTCTGCTCTTTTAAAAAAAAAATGTTCTTGGGACTAACTGAGTTGTGTTTGTTTTCACTAGTGATTTTAGCATGAAAAAGGATTCACCAATATTGGAAATCGAACTGGATGTGGATGAGATGGCGAAAAATCACAAGGCCTGAAAGAGAGTTTTATCTTGATTTGGACTTTGTATGTAATCAATCTCTTTTGCCAATTTTTCCTTGCAGATG

mRNA sequence

CACCTGCAAATTATCTTCTTGAAATGGCTTCCCTCTCCACCTGGTTCCGCTACATCGCCCACAAGCTCGAGTACTCCGTCTCTCTCAGTTGGAAGAACTATAAAGGAGGTCAAATTACTGACAAAGAAGTAGGTGATGCCGTTTGGAAGAATTTACTTCAGGGAAAGCTCACATACCTCCATTGGATTAAGGGAGAGGAGATGGCCCCAACTGTAGGTGAGGCAGGGGGAACGCTTCTCATACGCAAATTACCTGCTGCAGATCCAACGAGTGTTTTTGTTGGGGATGTCGTGGTGGTGAAGGAGCCAGAAAAACCAGAAAACTATCTTGTGAGAAGATTGGTTGCAGTTGAAGGGTATGAAATGCTGTCTACAGATGAAAAAGATAAGCCTTTCATGCTTGAGAAGGATCAGTGCTGGTTGTTGGCAGATAACGAGGAGTTGAAGCCGAAGGAGGCGTACGACAGTCGAACATTTGGTCCAGTTACGATGTCAGACATTGTAGGCAGAGCCATATATTGCCTACGAACAGCGGTCGACCATGGCCCAGTGCAGAATAGTGATTTTAGCATGAAAAAGGATTCACCAATATTGGAAATCGAACTGGATGTGGATGAGATGGCGAAAAATCACAAGGCCTGAAAGAGAGTTTTATCTTGATTTGGACTTTGTATGTAATCAATCTCTTTTGCCAATTTTTCCTTGCAGATG

Coding sequence (CDS)

ATGGCTTCCCTCTCCACCTGGTTCCGCTACATCGCCCACAAGCTCGAGTACTCCGTCTCTCTCAGTTGGAAGAACTATAAAGGAGGTCAAATTACTGACAAAGAAGTAGGTGATGCCGTTTGGAAGAATTTACTTCAGGGAAAGCTCACATACCTCCATTGGATTAAGGGAGAGGAGATGGCCCCAACTGTAGGTGAGGCAGGGGGAACGCTTCTCATACGCAAATTACCTGCTGCAGATCCAACGAGTGTTTTTGTTGGGGATGTCGTGGTGGTGAAGGAGCCAGAAAAACCAGAAAACTATCTTGTGAGAAGATTGGTTGCAGTTGAAGGGTATGAAATGCTGTCTACAGATGAAAAAGATAAGCCTTTCATGCTTGAGAAGGATCAGTGCTGGTTGTTGGCAGATAACGAGGAGTTGAAGCCGAAGGAGGCGTACGACAGTCGAACATTTGGTCCAGTTACGATGTCAGACATTGTAGGCAGAGCCATATATTGCCTACGAACAGCGGTCGACCATGGCCCAGTGCAGAATAGTGATTTTAGCATGAAAAAGGATTCACCAATATTGGAAATCGAACTGGATGTGGATGAGATGGCGAAAAATCACAAGGCCTGA

Protein sequence

MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEMAPTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEKDKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSDFSMKKDSPILEIELDVDEMAKNHKA

Homology

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BLAST of Carg11499 vs. NCBI nr
Match: XP_022954177.1 (mitochondrial inner membrane protease subunit 2-like [Cucurbita moschata] >KAG7014558.1 Mitochondrial inner membrane protease subunit 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.4 bits (1082), Expect = 4.5e-114
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK
Sbjct: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD
Sbjct: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMKKDSPILEIELDVDEMAKNHKA
Sbjct: 181 FSMKKDSPILEIELDVDEMAKNHKA 205

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BLAST of Carg11499 vs. NCBI nr
Match: KAG6576037.1 (hypothetical protein SDJN03_26676, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 418.3 bits (1074), Expect = 3.8e-113
Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQ KLTYLHWIKGEEM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQEKLTYLHWIKGEEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK
Sbjct: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD
Sbjct: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMKKDSPILEIELDVDEMAKNHKA
Sbjct: 181 FSMKKDSPILEIELDVDEMAKNHKA 205

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BLAST of Carg11499 vs. NCBI nr
Match: XP_023547664.1 (mitochondrial inner membrane protease subunit 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.2 bits (1071), Expect = 8.4e-113
Identity = 202/205 (98.54%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRL AVEGYEMLSTDEK
Sbjct: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLAAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           DKPF+LEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD
Sbjct: 121 DKPFVLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMKKDSPILEIELDVDEMA+NHKA
Sbjct: 181 FSMKKDSPILEIELDVDEMARNHKA 205

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BLAST of Carg11499 vs. NCBI nr
Match: XP_022991205.1 (mitochondrial inner membrane protease subunit 2-like [Cucurbita maxima])

HSP 1 Score: 413.7 bits (1062), Expect = 9.3e-112
Identity = 201/205 (98.05%), Postives = 203/205 (99.02%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVG AGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRL AVEGYEMLSTDEK
Sbjct: 61  APTVGVAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLAAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           DKPF+LEKDQCWLLADNEELKPKEAYDSRTFGPVTMS+IVGRAIYCLRTAVDHGPVQNSD
Sbjct: 121 DKPFVLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSNIVGRAIYCLRTAVDHGPVQNSD 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMKKDSPILEIELDVDEMAKNHKA
Sbjct: 181 FSMKKDSPILEIELDVDEMAKNHKA 205

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BLAST of Carg11499 vs. NCBI nr
Match: XP_038897309.1 (mitochondrial inner membrane protease subunit 2-like [Benincasa hispida])

HSP 1 Score: 400.2 bits (1027), Expect = 1.1e-107
Identity = 191/205 (93.17%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHW+KG EM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWVKGVEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVGEAGGTLL+RKLP ADPTSVFVGDVVVVKEPEKPENYLVRRL AVEG+EMLSTDE 
Sbjct: 61  APTVGEAGGTLLVRKLPTADPTSVFVGDVVVVKEPEKPENYLVRRLAAVEGHEMLSTDEN 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           D+PF+LEK+QCWLLADNE+LKPKEAYDSRTFGPV+MSDIVGRAIYCLRTAVDHGPVQNS+
Sbjct: 121 DEPFVLEKEQCWLLADNEKLKPKEAYDSRTFGPVSMSDIVGRAIYCLRTAVDHGPVQNSN 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMK+DSPILEIELDVDEMAKNHKA
Sbjct: 181 FSMKRDSPILEIELDVDEMAKNHKA 205

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BLAST of Carg11499 vs. ExPASy Swiss-Prot
Match: Q6AZD4 (Mitochondrial inner membrane protease subunit 2 OS=Danio rerio OX=7955 GN=immp2l PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.4e-06
Identity = 29/123 (23.58%), Postives = 63/123 (51.22%), Query Frame = 0


Query: 48  KLTYLHWIKGEEMAPTV---GEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVR 107
           +L Y+  ++G  M P++   GE+   +++    +     V  GD+V V  P+ P+  +++
Sbjct: 31  RLAYVARVEGASMQPSLNPDGESSPDVVLLNRWSVRNYHVQRGDIVSVLSPKNPQQKIIK 90

Query: 108 RLVAVEGYEMLSTDEKDKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAI 167
           R++ +EG  + +   K++   +     W+  D+       ++DS  FGPV++  + GRA 
Sbjct: 91  RVIGIEGDFIKTLGYKNRYVRVPDGHLWIEGDHH----GHSFDSNAFGPVSLGLVHGRAS 149

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BLAST of Carg11499 vs. ExPASy Swiss-Prot
Match: Q9CQU8 (Mitochondrial inner membrane protease subunit 1 OS=Mus musculus OX=10090 GN=Immp1l PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.0e-04
Identity = 31/105 (29.52%), Postives = 50/105 (47.62%), Query Frame = 0


Query: 87  GDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEKD---KPFMLEKDQCWLLADNEELKPK 146
           GD+V+ K P  P++ + +R++ +EG ++LST   D       +     WL  DN +    
Sbjct: 66  GDIVIAKSPSDPKSNICKRVIGLEGDKILSTSPSDVFKSRSYVPTGHVWLEGDNLQ---- 125

Query: 147 EAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSDFSMKKDSP 189
            + DSR +GP+    I GR  + +           SDF   +DSP
Sbjct: 126 NSTDSRYYGPIPYGLIRGRIFFKIWPF--------SDFGFLRDSP 158

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BLAST of Carg11499 vs. ExPASy Swiss-Prot
Match: Q28I39 (Mitochondrial inner membrane protease subunit 1 OS=Xenopus tropicalis OX=8364 GN=immp1l PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.3e-04
Identity = 28/100 (28.00%), Postives = 50/100 (50.00%), Query Frame = 0


Query: 83  SVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK---DKPFMLEKDQCWLLADNEE 142
           S+  GD++V K P+KP   + +R++ +EG ++  +       +   + K   WL  DN +
Sbjct: 62  SIHKGDIIVAKSPDKPSVNICKRVIGLEGDKVCMSSPSALLKRHTYVPKGHVWLEGDNLD 121

Query: 143 LKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNS 180
                + DSR++GPV  + I GR    +      GP++ S
Sbjct: 122 ----NSTDSRSYGPVPYALIRGRICLRVWPLESFGPLKES 157

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BLAST of Carg11499 vs. ExPASy Swiss-Prot
Match: Q96LU5 (Mitochondrial inner membrane protease subunit 1 OS=Homo sapiens OX=9606 GN=IMMP1L PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 5.1e-04
Identity = 26/91 (28.57%), Postives = 45/91 (49.45%), Query Frame = 0


Query: 87  GDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEKD---KPFMLEKDQCWLLADNEELKPK 146
           GD+V+ K P  P++ + +R++ +EG ++L+T   D       +     WL  DN +    
Sbjct: 66  GDIVIAKSPSDPKSNICKRVIGLEGDKILTTSPSDFFKSHSYVPMGHVWLEGDNLQ---- 125

Query: 147 EAYDSRTFGPVTMSDIVGRAIYCLRTAVDHG 175
            + DSR +GP+    I GR  + +    D G
Sbjct: 126 NSTDSRCYGPIPYGLIRGRIFFKIWPLSDFG 152

[Close]

BLAST of Carg11499 vs. ExPASy Swiss-Prot
Match: Q96T52 (Mitochondrial inner membrane protease subunit 2 OS=Homo sapiens OX=9606 GN=IMMP2L PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 5.1e-04
Identity = 27/117 (23.08%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 55  IKGEEMAPTVGEAGG----TLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVE 114
           ++G  M P++   G      +L+      +   V  GD+V +  P+ PE  +++R++A+E
Sbjct: 39  VEGASMQPSLNPGGSQSSDVVLLNHWKVRN-FEVHRGDIVSLVSPKNPEQKIIKRVIALE 98

Query: 115 GYEMLSTDEKDKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCL 168
           G  + +   K++   + +   W+  D+       ++DS +FGPV++  +   A + L
Sbjct: 99  GDIVRTIGHKNRYVKVPRGHIWVEGDHH----GHSFDSNSFGPVSLGLLHAHATHIL 150

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BLAST of Carg11499 vs. ExPASy TrEMBL
Match: A0A6J1GQA3 (mitochondrial inner membrane protease subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456518 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.2e-114
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK
Sbjct: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD
Sbjct: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMKKDSPILEIELDVDEMAKNHKA
Sbjct: 181 FSMKKDSPILEIELDVDEMAKNHKA 205

[Close]

BLAST of Carg11499 vs. ExPASy TrEMBL
Match: A0A6J1JS86 (mitochondrial inner membrane protease subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487930 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 4.5e-112
Identity = 201/205 (98.05%), Postives = 203/205 (99.02%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVG AGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRL AVEGYEMLSTDEK
Sbjct: 61  APTVGVAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLAAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           DKPF+LEKDQCWLLADNEELKPKEAYDSRTFGPVTMS+IVGRAIYCLRTAVDHGPVQNSD
Sbjct: 121 DKPFVLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSNIVGRAIYCLRTAVDHGPVQNSD 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMKKDSPILEIELDVDEMAKNHKA
Sbjct: 181 FSMKKDSPILEIELDVDEMAKNHKA 205

[Close]

BLAST of Carg11499 vs. ExPASy TrEMBL
Match: A0A6J1H7P2 (uncharacterized protein LOC111461243 OS=Cucurbita moschata OX=3662 GN=LOC111461243 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.2e-106
Identity = 189/205 (92.20%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHW KG EM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWFKGVEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVGE GGTLL+RKLP A+PTSVFVGDVVVVKEPEKPENYLVRRL AVEGYEMLSTDEK
Sbjct: 61  APTVGETGGTLLVRKLPVANPTSVFVGDVVVVKEPEKPENYLVRRLAAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           D+PF+LEKD+CWLLA+NE+LKPKEAYDSRTFGPV MSDIVGRAIYCLRTAVDHGPVQNS 
Sbjct: 121 DEPFILEKDECWLLAENEKLKPKEAYDSRTFGPVPMSDIVGRAIYCLRTAVDHGPVQNSY 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           +SMK+DSPILEIELDVDEMAKNHKA
Sbjct: 181 YSMKRDSPILEIELDVDEMAKNHKA 205

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BLAST of Carg11499 vs. ExPASy TrEMBL
Match: A0A6J1KT69 (uncharacterized protein LOC111498007 OS=Cucurbita maxima OX=3661 GN=LOC111498007 PE=4 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.1e-105
Identity = 188/205 (91.71%), Postives = 197/205 (96.10%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHW KG EM
Sbjct: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWFKGVEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           A TVGE GGTLL+RKLP A+PTSVFVGDVVVVKEPEKPENYLVRRL AVEGYEMLSTDEK
Sbjct: 61  ALTVGETGGTLLVRKLPVANPTSVFVGDVVVVKEPEKPENYLVRRLAAVEGYEMLSTDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           D+PF+LEKD+CWLLA+NE+LKPKEAYDSRTFGPV MSDIVGRAIYCLRTAVDHGPVQNS 
Sbjct: 121 DEPFILEKDECWLLAENEKLKPKEAYDSRTFGPVPMSDIVGRAIYCLRTAVDHGPVQNSY 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           +SMK+DSPILEIELDVDEMAKNHKA
Sbjct: 181 YSMKRDSPILEIELDVDEMAKNHKA 205

[Close]

BLAST of Carg11499 vs. ExPASy TrEMBL
Match: A0A1S3BH09 (uncharacterized protein LOC103489739 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489739 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.6e-104
Identity = 185/205 (90.24%), Postives = 200/205 (97.56%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MAS STW RYIA+KLE+SVSLSWKNYKGG+ITD+EVGDAVWKNLLQGKLTYLHWIKG+EM
Sbjct: 43  MASFSTWLRYIANKLEFSVSLSWKNYKGGRITDREVGDAVWKNLLQGKLTYLHWIKGQEM 102

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APTVGEAGGTLL+RKLP A+PTSV+VGDVVVVKEPEKPENYLVRRL AVEG+EMLSTDEK
Sbjct: 103 APTVGEAGGTLLVRKLPDANPTSVYVGDVVVVKEPEKPENYLVRRLAAVEGHEMLSTDEK 162

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           D+PF LEKDQCWLLADNE+LKPKEAYDSRTFGPV+MSDIVGRAIYCLR+AVDHGPVQNS+
Sbjct: 163 DQPFTLEKDQCWLLADNEKLKPKEAYDSRTFGPVSMSDIVGRAIYCLRSAVDHGPVQNSN 222

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           FSMK+DSPILEIELDVDEMAKNHKA
Sbjct: 223 FSMKRDSPILEIELDVDEMAKNHKA 247

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BLAST of Carg11499 vs. TAIR 10
Match: AT2G31140.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 282.7 bits (722), Expect = 2.3e-76
Identity = 133/205 (64.88%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MAS+STWFRY+AHKLEYS++LS K+++  +++D+E+   + KNL  GK+TYLH  KG EM
Sbjct: 1   MASISTWFRYMAHKLEYSLTLSLKSHRSNKLSDRELIQIICKNLFYGKITYLHSDKGPEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           +PT+     TLLIRK+P A+   VF+GD VV+K+P   + YLVRRL AVEG+EM+S DEK
Sbjct: 61  SPTMTANENTLLIRKIPIANTRFVFIGDAVVLKDPNDSDKYLVRRLAAVEGFEMVSGDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           ++PF+LEK+QCW+ A+N+ELK KEAYDSRTFGPV+ +DIVGRAIYCLRTAVDHGPV+NS 
Sbjct: 121 EEPFVLEKNQCWVTAENQELKAKEAYDSRTFGPVSTADIVGRAIYCLRTAVDHGPVRNSQ 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
            +M +DSPIL +ELDVDEMAKNHKA
Sbjct: 181 TAMGQDSPILAVELDVDEMAKNHKA 205

[Close]

BLAST of Carg11499 vs. TAIR 10
Match: AT1G06200.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 278.1 bits (710), Expect = 5.7e-75
Identity = 128/205 (62.44%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 1   MASLSTWFRYIAHKLEYSVSLSWKNYKGGQITDKEVGDAVWKNLLQGKLTYLHWIKGEEM 60
           MAS++ + RY+AHKLEYS++LS K +   +++D+E+   V KNL  G+++YLH  KG+EM
Sbjct: 1   MASITNYVRYMAHKLEYSLTLSLKKHTREKLSDRELFGVVMKNLFYGRISYLHSDKGKEM 60

Query: 61  APTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEMLSTDEK 120
           APT+G    TLL+RKLP  D   +FVGD VV+K+P +   Y+VRRL A+EG EM+S+DEK
Sbjct: 61  APTMGTNESTLLVRKLPVVDTRYIFVGDAVVLKDPNETNKYIVRRLAALEGSEMVSSDEK 120

Query: 121 DKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAIYCLRTAVDHGPVQNSD 180
           D+PF+LEKDQCW++A+N+E+K KEAYDSRTFGP++M+DIVGRAIYCLRTAVDHGPV NS+
Sbjct: 121 DEPFVLEKDQCWVVAENQEMKSKEAYDSRTFGPISMADIVGRAIYCLRTAVDHGPVSNSE 180

Query: 181 FSMKKDSPILEIELDVDEMAKNHKA 206
           F+M +DSPIL +ELDVDE+AK HKA
Sbjct: 181 FAMDEDSPILAVELDVDELAKGHKA 205

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BLAST of Carg11499 vs. TAIR 10
Match: AT1G29960.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-06
Identity = 35/136 (25.74%), Postives = 63/136 (46.32%), Query Frame = 0


Query: 49  LTYLHWIKGEEMAPTVGEAGGTLLIRKLPA--ADPTSVFVGDVVVVKEPEKPENYLVRRL 108
           L ++ +  G  M PT+  +G  LL  ++      P+    GD+VV++ PE P    ++R+
Sbjct: 37  LGFMAYAYGPSMTPTLHPSGNVLLAERISKRYQKPSR---GDIVVIRSPENPNKTPIKRV 96

Query: 109 VAVEG------YEMLSTDEKDKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIV 168
           + +EG       +   +DE  +  ++ K   ++  D        + DSR FG V    I 
Sbjct: 97  IGIEGDCISFVIDSRKSDE-SQTIVVPKGHVFVQGDYTH----NSRDSRNFGTVPYGLIQ 156

Query: 169 GRAIYCLRTAVDHGPV 177
           GR ++ +    D GP+
Sbjct: 157 GRVLWRVWPFQDFGPL 164

[Close]

BLAST of Carg11499 vs. TAIR 10
Match: AT1G23465.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-06
Identity = 35/125 (28.00%), Postives = 60/125 (48.00%), Query Frame = 0


Query: 49  LTYLHWIKGEEMAPTVGEAGGTLLIRKLPA--ADPTSVFVGDVVVVKEPEKPENYLVRRL 108
           L ++ +  G  M PT+  +G  LL  ++      P+    GD+VV++ PE P    ++R+
Sbjct: 37  LGFMAYAYGPSMIPTLHPSGNMLLAERISKRYQKPSR---GDIVVIRSPENPNKTPIKRV 96

Query: 109 VAVEG------YEMLSTDEKDKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIV 166
           V VEG       + + +DE  +  ++ K   ++  D        + DSR FGPV    I 
Sbjct: 97  VGVEGDCISFVIDPVKSDE-SQTIVVPKGHVFVQGDYTH----NSRDSRNFGPVPYGLIQ 153

[Close]

BLAST of Carg11499 vs. TAIR 10
Match: AT1G53530.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-06
Identity = 34/115 (29.57%), Postives = 56/115 (48.70%), Query Frame = 0


Query: 55  IKGEEMAPTVGEAGGTLLIRKLPAADPTSVFVGDVVVVKEPEKPENYLVRRLVAVEGYEM 114
           + G  M PT+   G  +L   L +     + +GDVV+V+ P  P+  + +R++ +EG  +
Sbjct: 46  VHGPSMLPTLNLTGDVILAEHL-SHRFGKIGLGDVVLVRSPRDPKRMVTKRILGLEGDRL 105

Query: 115 -LSTD----EKDKPFMLEKDQCWLLADNEELKPKEAYDSRTFGPVTMSDIVGRAI 165
             S D    +     ++ K   W+  DN       + DSR FGPV  S I G+A+
Sbjct: 106 TFSADPLVGDASVSVLVPKGHVWIQGDN----LYASTDSRHFGPVPYSLIEGKAL 155

The following BLAST results are available for this feature:

BLAST of Carg11499 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022954177.1	4.5e-114	100.00	mitochondrial inner membrane protease subunit 2-like [Cucurbita moschata] >KAG70...	[more]
KAG6576037.1	3.8e-113	99.51	hypothetical protein SDJN03_26676, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023547664.1	8.4e-113	98.54	mitochondrial inner membrane protease subunit 2-like [Cucurbita pepo subsp. pepo...	[more]
XP_022991205.1	9.3e-112	98.05	mitochondrial inner membrane protease subunit 2-like [Cucurbita maxima]	[more]
XP_038897309.1	1.1e-107	93.17	mitochondrial inner membrane protease subunit 2-like [Benincasa hispida]	[more]

BLAST of Carg11499 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AZD4	9.4e-06	23.58	Mitochondrial inner membrane protease subunit 2 OS=Danio rerio OX=7955 GN=immp2l...	[more]
Q9CQU8	1.0e-04	29.52	Mitochondrial inner membrane protease subunit 1 OS=Mus musculus OX=10090 GN=Immp...	[more]
Q28I39	2.3e-04	28.00	Mitochondrial inner membrane protease subunit 1 OS=Xenopus tropicalis OX=8364 GN...	[more]
Q96LU5	5.1e-04	28.57	Mitochondrial inner membrane protease subunit 1 OS=Homo sapiens OX=9606 GN=IMMP1...	[more]
Q96T52	5.1e-04	23.08	Mitochondrial inner membrane protease subunit 2 OS=Homo sapiens OX=9606 GN=IMMP2...	[more]

BLAST of Carg11499 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GQA3	2.2e-114	100.00	mitochondrial inner membrane protease subunit 2-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1JS86	4.5e-112	98.05	mitochondrial inner membrane protease subunit 2-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1H7P2	2.2e-106	92.20	uncharacterized protein LOC111461243 OS=Cucurbita moschata OX=3662 GN=LOC1114612...	[more]
A0A6J1KT69	3.1e-105	91.71	uncharacterized protein LOC111498007 OS=Cucurbita maxima OX=3661 GN=LOC111498007...	[more]
A0A1S3BH09	1.6e-104	90.24	uncharacterized protein LOC103489739 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Carg11499 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31140.1	2.3e-76	64.88	Peptidase S24/S26A/S26B/S26C family protein	[more]
AT1G06200.1	5.7e-75	62.44	Peptidase S24/S26A/S26B/S26C family protein	[more]
AT1G29960.1	7.4e-06	25.74	Peptidase S24/S26A/S26B/S26C family protein	[more]
AT1G23465.1	7.4e-06	28.00	Peptidase S24/S26A/S26B/S26C family protein	[more]
AT1G53530.1	7.4e-06	29.57	Peptidase S24/S26A/S26B/S26C family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.10.109.10	Umud Fragment, subunit A	coord: 47..180 e-value: 8.0E-16 score: 60.1
None	No IPR available	PANTHER	PTHR47040	OSJNBA0068L06.9 PROTEIN	coord: 1..205
IPR019533	Peptidase S26	CDD	cd06530	S26_SPase_I	coord: 55..163 e-value: 3.32039E-5 score: 39.1059
IPR036286	LexA/Signal peptidase-like superfamily	SUPERFAMILY	51306	LexA/Signal peptidase	coord: 51..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11499-RA	Carg11499-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006465	signal peptide processing
molecular_function	GO:0004252	serine-type endopeptidase activity