Carg11416 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11416
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description9-cis-epoxycarotenoid dioxygenase, putative
LocationCarg_Chr16: 2837784 .. 2839568 (+)
RNA-Seq ExpressionCarg11416
SyntenyCarg11416
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCCATGTCTTCCCCTTTCCTCTCCGGCGGAAACCTCCTCCTCTCTCCGCCGATCTCCAAGTCCAGGACCGCCGTTTCGACCGCCATATGCTCTGTACTCGCCGAAGAAACCCCAAGAACCGTTAAAAAACCCGCCTCCCGACCAGATGCTGATTTTCCATCGCCGTCTTTGTCCCAGCGAGCTACTCCCCCTCCGGCAGTGGCGAAGGGCTCTTCCACCAGACGTGTCGAGCCATCTGTACCGGCGAGCATCTTCAATGCCTTTGATGACTTGATCAACAACTTCATAAACCCGCCGGCGAATCCGTCGACGGATCCACGATACGTCCTTGCTGACAACTTTGCTCCCGTCGACGAGCTTCCACCGACGGAGTGTGAAGTCATTCAAGGTTCGTTGCCTTCATCTCTCAATGGTGCGTATATTCGTAACGGCCCGAACCCTCAATACCTTCCCCGTGGGCCCTACCACTTGTTCGACGGCGATGGCATGCTTCACTCTCTTCGAATCTCCAACGGCCGTGCCGTTCTGTGCAGCCGTTATGTGAAAACGTACAAGTACACTTTGGAGCGTGACGCTGGACATCCTGTTATCCCCAACGTATTCTCCGGCTTCAATGGCCTCATCGCCTCTGCCGCACGCGGCGCCGTTACCGCCGGTCGGATGTTTACCGGCCAATTTAATCCGACAAATGGGATTGGTCTTGCAAACACTAGTTTGGCCTTCTTCGGTGATCGTCTTTACGCCCTAGGTGAGTCCGATTTACCGTATCCAATTCGATTGACTCCAAACGGCGACATTGAAACTCTCGGCCGCCATGATTTTGACGGAAAGCTTTCCATGAGTATGACTGCTCATCCAAAAATCGATTACGATACGGGTGAGGCATTTGCGTTTCGGTATGGTCCTCTGCCTCCGTTCTTAACCTTCTTTCGATTCGACAAGAACGGAGCCAAACAGCCGGATGTGCGGATATTTTCGATGAATCGACCTTCGTTCCTGCACGATTTTGCAATTACTAAAAAGTACGCTGTATTTACAGAGATTCAGATCGGATTTAACCCTATGCAAATGATAGTAGAAGGACGCTCTCCAGTTGGAGCAGATCCTTCAAAAATTTCAAGAATAGGACTAATTCCACGATACGCTAACGACGAATCGAAGATGAAGTGGTTTGATGTACCTGGAGTTAACCTGGTTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGATTCTGGTGGCGCCGAACGTTCTTTCCGTCGAACACACGATGGAGAGGATGGATCTGGTTCACGCCTTAGTGGAGAAAATCAGAATCGACCTAAAAACAGGGATAATAACGCGGCGGCCATTGTCGACCAGAAACCTAGACTTCGGAGTGATAAATCCGCTACACGTTGGGAAGAAGAATAGATTCGTTTACGCCGGCGTTGGAGATCCGATGCCGAAGATTTCGGGAGTTGTGAAGCTCGACGTGTCTCAGGAAGAACGCCGAGACTGTATCGTCGCGACTAGGCTTTTCGGACCTAATTGCTACGGCGGAGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAATTCCGACAAAACGGCGGCGGAGGAGGACGACGGATATGTCGTCTCGTACGTCCACGATGAAAACTCGGGCGACTCAAGGTTCGTAGTCATGGACGCCAAGTCCCCGGAACTCGAGATTATCGCCACCGTGAAGCTGCCGCGGCGTGTCCCTTACGGCTTTCACGGCCTGTTTGTGAAAGAAAGTGACCTCTCTAAGCTATAA

mRNA sequence

ATGGACGCCATGTCTTCCCCTTTCCTCTCCGGCGGAAACCTCCTCCTCTCTCCGCCGATCTCCAAGTCCAGGACCGCCGTTTCGACCGCCATATGCTCTGTACTCGCCGAAGAAACCCCAAGAACCGTTAAAAAACCCGCCTCCCGACCAGATGCTGATTTTCCATCGCCGTCTTTGTCCCAGCGAGCTACTCCCCCTCCGGCAGTGGCGAAGGGCTCTTCCACCAGACGTGTCGAGCCATCTGTACCGGCGAGCATCTTCAATGCCTTTGATGACTTGATCAACAACTTCATAAACCCGCCGGCGAATCCGTCGACGGATCCACGATACGTCCTTGCTGACAACTTTGCTCCCGTCGACGAGCTTCCACCGACGGAGTGTGAAGTCATTCAAGGTTCGTTGCCTTCATCTCTCAATGGTGCGTATATTCGTAACGGCCCGAACCCTCAATACCTTCCCCGTGGGCCCTACCACTTGTTCGACGGCGATGGCATGCTTCACTCTCTTCGAATCTCCAACGGCCGTGCCGTTCTGTGCAGCCGTTATGTGAAAACGTACAAGTACACTTTGGAGCGTGACGCTGGACATCCTGTTATCCCCAACGTATTCTCCGGCTTCAATGGCCTCATCGCCTCTGCCGCACGCGGCGCCGTTACCGCCGGTCGGATGTTTACCGGCCAATTTAATCCGACAAATGGGATTGGTCTTGCAAACACTAGTTTGGCCTTCTTCGGTGATCGTCTTTACGCCCTAGGTGAGTCCGATTTACCGTATCCAATTCGATTGACTCCAAACGGCGACATTGAAACTCTCGGCCGCCATGATTTTGACGGAAAGCTTTCCATGAGTATGACTGCTCATCCAAAAATCGATTACGATACGGGTGAGGCATTTGCGTTTCGGTATGGTCCTCTGCCTCCGTTCTTAACCTTCTTTCGATTCGACAAGAACGGAGCCAAACAGCCGGATGTGCGGATATTTTCGATGAATCGACCTTCGTTCCTGCACGATTTTGCAATTACTAAAAAGTACGCTGTATTTACAGAGATTCAGATCGGATTTAACCCTATGCAAATGATAGTAGAAGGACGCTCTCCAGTTGGAGCAGATCCTTCAAAAATTTCAAGAATAGGACTAATTCCACGATACGCTAACGACGAATCGAAGATGAAGTGGTTTGATGTACCTGGAGTTAACCTGGTTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGATTCTGGTGGCGCCGAACGTTCTTTCCGTCGAACACACGATGGAGAGGATGGATCTGGTTCACGCCTTAGTGGAGAAAATCAGAATCGACCTAAAAACAGGGATAATAACGCGGCGGCCATTGTCGACCAGAAACCTAGACTTCGGAGTGATAAATCCGCTACACGTTGGGAAGAAGAATAGATTCGTTTACGCCGGCGTTGGAGATCCGATGCCGAAGATTTCGGGAGTTGTGAAGCTCGACGTGTCTCAGGAAGAACGCCGAGACTGTATCGTCGCGACTAGGCTTTTCGGACCTAATTGCTACGGCGGAGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAATTCCGACAAAACGGCGGCGGAGGAGGACGACGGATATGTCGTCTCGTACGTCCACGATGAAAACTCGGGCGACTCAAGGTTCGTAGTCATGGACGCCAAGTCCCCGGAACTCGAGATTATCGCCACCGTGAAGCTGCCGCGGCGTGTCCCTTACGGCTTTCACGGCCTGTTTGTGAAAGAAAGTGACCTCTCTAAGCTATAA

Coding sequence (CDS)

ATGGACGCCATGTCTTCCCCTTTCCTCTCCGGCGGAAACCTCCTCCTCTCTCCGCCGATCTCCAAGTCCAGGACCGCCGTTTCGACCGCCATATGCTCTGTACTCGCCGAAGAAACCCCAAGAACCGTTAAAAAACCCGCCTCCCGACCAGATGCTGATTTTCCATCGCCGTCTTTGTCCCAGCGAGCTACTCCCCCTCCGGCAGTGGCGAAGGGCTCTTCCACCAGACGTGTCGAGCCATCTGTACCGGCGAGCATCTTCAATGCCTTTGATGACTTGATCAACAACTTCATAAACCCGCCGGCGAATCCGTCGACGGATCCACGATACGTCCTTGCTGACAACTTTGCTCCCGTCGACGAGCTTCCACCGACGGAGTGTGAAGTCATTCAAGGTTCGTTGCCTTCATCTCTCAATGGTGCGTATATTCGTAACGGCCCGAACCCTCAATACCTTCCCCGTGGGCCCTACCACTTGTTCGACGGCGATGGCATGCTTCACTCTCTTCGAATCTCCAACGGCCGTGCCGTTCTGTGCAGCCGTTATGTGAAAACGTACAAGTACACTTTGGAGCGTGACGCTGGACATCCTGTTATCCCCAACGTATTCTCCGGCTTCAATGGCCTCATCGCCTCTGCCGCACGCGGCGCCGTTACCGCCGGTCGGATGTTTACCGGCCAATTTAATCCGACAAATGGGATTGGTCTTGCAAACACTAGTTTGGCCTTCTTCGGTGATCGTCTTTACGCCCTAGGTGAGTCCGATTTACCGTATCCAATTCGATTGACTCCAAACGGCGACATTGAAACTCTCGGCCGCCATGATTTTGACGGAAAGCTTTCCATGAGTATGACTGCTCATCCAAAAATCGATTACGATACGGGTGAGGCATTTGCGTTTCGGTATGGTCCTCTGCCTCCGTTCTTAACCTTCTTTCGATTCGACAAGAACGGAGCCAAACAGCCGGATGTGCGGATATTTTCGATGAATCGACCTTCGTTCCTGCACGATTTTGCAATTACTAAAAAGTACGCTGTATTTACAGAGATTCAGATCGGATTTAACCCTATGCAAATGATAGTAGAAGGACGCTCTCCAGTTGGAGCAGATCCTTCAAAAATTTCAAGAATAGGACTAATTCCACGATACGCTAACGACGAATCGAAGATGAAGTGGTTTGATGTACCTGGAGTTAACCTGGTTCACGCCATTAACGCTTGGGATGAAGACGACGCTGTGATTCTGGTGGCGCCGAACGTTCTTTCCGTCGAACACACGATGGAGAGGATGGATCTGGTTCACGCCTTAGTGGAGAAAATCAGAATCGACCTAAAAACAGGGATAATAACGCGGCGGCCATTGTCGACCAGAAACCTAGACTTCGGAGTGATAAATCCGCTACACGTTGGGAAGAAGAATAGATTCGTTTACGCCGGCGTTGGAGATCCGATGCCGAAGATTTCGGGAGTTGTGAAGCTCGACGTGTCTCAGGAAGAACGCCGAGACTGTATCGTCGCGACTAGGCTTTTCGGACCTAATTGCTACGGCGGAGAGCCGTTTTTCGTGCCGAGGGAAAGGGAAAATTCCGACAAAACGGCGGCGGAGGAGGACGACGGATATGTCGTCTCGTACGTCCACGATGAAAACTCGGGCGACTCAAGGTTCGTAGTCATGGACGCCAAGTCCCCGGAACTCGAGATTATCGCCACCGTGAAGCTGCCGCGGCGTGTCCCTTACGGCTTTCACGGCCTGTTTGTGAAAGAAAGTGACCTCTCTAAGCTATAA

Protein sequence

MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLSQRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAFRYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Homology
BLAST of Carg11416 vs. NCBI nr
Match: KAG7015104.1 (putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Carg11416 vs. NCBI nr
Match: XP_022931473.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata] >AXM43901.1 carotenoid cleavage dioxygenase [Cucurbita moschata])

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 587/594 (98.82%), Postives = 591/594 (99.49%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFLSGGNLLLSP ISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAISSPFLSGGNLLLSPSISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQPDV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPL+VGKKNRFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSD+ AAEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDEMAAEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Carg11416 vs. NCBI nr
Match: XP_022985089.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita maxima] >AXM43899.1 carotenoid cleavage dioxygenase [Cucurbita maxima])

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 580/594 (97.64%), Postives = 587/594 (98.82%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFLSGGNLLLSPP SK RTAVSTAICSVL EETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAISSPFLSGGNLLLSPPTSKYRTAVSTAICSVLTEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRAT PPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD
Sbjct: 61  QRATHPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPANGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQPDV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDE+DAVILVAPN+
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEEDAVILVAPNI 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINP++VGKKNRFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPVYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVATRLFGPN YGGEPFFVPRERE+SD+TAAEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNYYGGEPFFVPRERESSDETAAEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Carg11416 vs. NCBI nr
Match: XP_023553272.1 (LOW QUALITY PROTEIN: probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 583/596 (97.82%), Postives = 588/596 (98.66%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFLSGGNLLLS PISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRATPPPAVAKGSS RRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSARRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTEC+VIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECDVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHP  PNVFSGFNGLIASAARGAVTAGRMFTGQFNP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHP-XPNVFSGFNGLIASAARGAVTAGRMFTGQFNPXNGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQ--PDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQ 360
           RYGPLPPFLTFFRFDKNGAKQ  PDV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQ
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQ 360

Query: 361 MIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAP 420
           MIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAP
Sbjct: 361 MIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAP 420

Query: 421 NVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYA 480
           N+LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPL+VGKKNRFVYA
Sbjct: 421 NILSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDD 540
           GVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSD+TAAEEDD
Sbjct: 481 GVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAAEEDD 540

Query: 541 GYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           GYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 GYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595

BLAST of Carg11416 vs. NCBI nr
Match: AXM43896.1 (carotenoid cleavage dioxygenase [Cucurbita pepo])

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 577/594 (97.14%), Postives = 585/594 (98.48%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFLSGGNLLLS PISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPP +PS DPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPV PNVFSGFNGL ASAARGAV AGR+ TGQ+NP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           +AFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 VAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQPDV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPN+
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNI 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPL+VGKKNRFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSD+TAAEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAAEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Carg11416 vs. ExPASy Swiss-Prot
Match: O49675 (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD4 PE=1 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 2.6e-226
Identity = 379/602 (62.96%), Postives = 473/602 (78.57%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAI---CSVLAEETPRTVKKPASRPDADFPSP 60
           MD++SS         L   + + R++  T +    +V+ E +P  +  P+   D      
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSP--ITNPSDNNDRRNKPK 60

Query: 61  SLSQRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFA 120
           +L  R      +       R E ++  ++F   +D+IN FI+PP+ PS DP++VL+DNFA
Sbjct: 61  TLHNRTN--HTLVSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFA 120

Query: 121 PV-DELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRA 180
           PV DELPPT+CE+I G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I NG+A
Sbjct: 121 PVLDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKA 180

Query: 181 VLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGL 240
            LCSRYVKTYKY +E+  G PV+PNVFSGFNG+ AS ARGA+TA R+ TGQ+NP NGIGL
Sbjct: 181 TLCSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGL 240

Query: 241 ANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGE 300
           ANTSLAFF +RL+ALGESDLPY +RLT +GDIET+GR+DFDGKL+MSMTAHPK D  TGE
Sbjct: 241 ANTSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGE 300

Query: 301 AFAFRYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIG--F 360
            FAFRYGP+PPFLT+FRFD  G KQ DV IFSM  PSFLHDFAITK++A+F EIQ+G   
Sbjct: 301 TFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRM 360

Query: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDD--A 420
           N + +++EG SPVG D  K  R+G+IP+YA DES+MKWF+VPG N++HAINAWDEDD  +
Sbjct: 361 NMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNS 420

Query: 421 VILVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKK 480
           V+L+APN++S+EHT+ERMDLVHALVEK++IDL TGI+ R P+S RNLDF VINP  +G+ 
Sbjct: 421 VVLIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRC 480

Query: 481 NRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKT 540
           +R+VYA +GDPMPKISGVVKLDVS+ +R DC VA R++G  CYGGEPFFV R+  N +  
Sbjct: 481 SRYVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARDPGNPE-- 540

Query: 541 AAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLS 595
            AEEDDGYVV+YVHDE +G+S+F+VMDAKSPELEI+A V+LPRRVPYGFHGLFVKESDL+
Sbjct: 541 -AEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLN 595

BLAST of Carg11416 vs. ExPASy Swiss-Prot
Match: Q6YVJ0 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.9e-193
Identity = 342/591 (57.87%), Postives = 431/591 (72.93%), Query Frame = 0

Query: 14  LLLSPPISKSRTAVSTAICS---VLAEETPRTVKKPASRPDADFPSPSLSQRATPPPAVA 73
           +L SP ++ +RT   T       V   +T RTV    +   A  PS S  +R   P A A
Sbjct: 57  VLTSPLVTTTRTPKQTEQEDEQLVAKTKTTRTVIATTNGRAA--PSQSRPRRRPAPAAAA 116

Query: 74  KGSSTRRVEPSVPASIFNAFDDLINNFINPPA-NPSTDPRYVLADNFAPVDELPPTECEV 133
             +       S+P +  NA +++IN FI+PPA  P+ DPR VL  NF PVDELPPT C V
Sbjct: 117 SAA-------SLPMTFCNALEEVINTFIDPPALRPAVDPRNVLTSNFVPVDELPPTPCPV 176

Query: 134 IQGSLPSSL-NGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI-----SNGRAVLCSRYV 193
           ++G++P  L  GAYIRNGPNPQ+LPRGP+HLFDGDGMLHSL +     S    VLCSRYV
Sbjct: 177 VRGAIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSPASSGDDPVLCSRYV 236

Query: 194 KTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTSLAF 253
           +TYKY +ERDAG PV+PNVFSGF+G +A  ARGAV A R+ TGQ NP  G+GLANTSLA+
Sbjct: 237 QTYKYLVERDAGAPVLPNVFSGFHG-VAGMARGAVVAARVLTGQMNPLEGVGLANTSLAY 296

Query: 254 FGDRLYALGESDLPYPIRLTPN-GDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAFRY 313
           F  RLYALGESDLPY +R+ P+ G++ T GR DF G+L M MTAHPK D  TGE FAFRY
Sbjct: 297 FAGRLYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPKKDPVTGELFAFRY 356

Query: 314 GPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMIVE 373
           GP+PPF+T+FRFD  G K  DV IFS+ +PSFLHDFAIT++YA+F EIQI   PM M+V 
Sbjct: 357 GPVPPFVTYFRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPEIQIVMKPMDMVVG 416

Query: 374 GRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDE--DDAVILVAPNV 433
           G SPVG+DP K+ R+G+IPRYA DES+M+WF+VPG N++H++NAW+E   + ++LVAPNV
Sbjct: 417 GGSPVGSDPGKVPRLGVIPRYATDESEMRWFEVPGFNIMHSVNAWEEAGGEELVLVAPNV 476

Query: 434 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 493
           LS+EH +E M+LVH+ VEK+RI+L+TG++TR PL+  N DF VINP  +G++NR+ Y GV
Sbjct: 477 LSIEHALEHMELVHSCVEKVRINLRTGVVTRTPLAAGNFDFPVINPAFLGRRNRYGYFGV 536

Query: 494 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 553
           GDP PKI GV KLD  +    DC VA R FGP C+ GEPFFV  + E +      EDDGY
Sbjct: 537 GDPAPKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFVADDVEGN----GNEDDGY 596

Query: 554 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDL 592
           +V YVHDE +G++RFVVMDA+SP+LEI+A V+LP RVPYGFHGLFV +++L
Sbjct: 597 LVCYVHDEATGENRFVVMDARSPDLEIVAEVQLPGRVPYGFHGLFVTQAEL 633

BLAST of Carg11416 vs. ExPASy Swiss-Prot
Match: Q5MBR6 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 5.1e-161
Identity = 286/523 (54.68%), Postives = 366/523 (69.98%), Query Frame = 0

Query: 81  SVPASIFNAFDDLINNFIN-PPANPSTDPRYVLADNFAPVDELPPTECEVIQGSLPSSL- 140
           S+P +I N  D +IN FI+ P   P+ DPR VL+ NFAPVDELPPT C VI+GS+PS L 
Sbjct: 55  SLPMAICNTVDKVINRFIDLPEQRPTVDPRRVLSGNFAPVDELPPTSCHVIRGSIPSCLA 114

Query: 141 NGAYIRNGPNPQY-LPRGPYHLFDGDGMLHSLRISNGRA------VLCSRYVKTYKYTLE 200
            G YIRNGPNPQ+ LP+  +HLFDGDGMLHSL I +  +      VLCSRYV TYKY LE
Sbjct: 115 GGVYIRNGPNPQHRLPQRTHHLFDGDGMLHSLLIPSASSTLLSEPVLCSRYVHTYKYLLE 174

Query: 201 RDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTSLAFFGDRLYAL 260
           R+ G PV+PN F+GF+G +A  AR  V   R+  GQ N   G GLANTS+  F D LYAL
Sbjct: 175 RETGGPVLPNFFAGFHG-VAGLARAVVMIARVLAGQINLNKGFGLANTSITLFADCLYAL 234

Query: 261 GESDLPYPIRLTP-NGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAFRYGPLPPFLT 320
            ESDLPY + + P NG++ TLGR DF G LS  MTAHPK D  T E FAFRY    PF+T
Sbjct: 235 CESDLPYSMHINPANGEVTTLGRCDFGGDLSFRMTAHPKKDPVTMELFAFRYNVFQPFIT 294

Query: 321 FFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMIVEGRSPVGAD 380
           +F FD+ G+K  DV I S+ +PS +HDFAIT++YA+F E Q+  NPM M++ G S VG D
Sbjct: 295 YFWFDRAGSKVADVPILSLQKPSVMHDFAITERYAIFPESQLIVNPMDMVMRGSSLVGLD 354

Query: 381 PSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDA--VILVAPNVLSVEHTME 440
            + + RIG++PRYA DES M+WF+VP  N++H  N W+E D   ++LVAPN+LS+EH + 
Sbjct: 355 RTMVPRIGVLPRYAKDESDMRWFEVPRFNMLHTTNGWEEADGEEIVLVAPNILSIEHMLG 414

Query: 441 RMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGVGDPMPKIS 500
            M+L+ A V+ +RI+L TG ++   LS  +L+FGVI+  +VG+KNR+ Y GV  P+PKI 
Sbjct: 415 NMELMRARVDMVRINLCTGDVSCTALSPESLEFGVIHQGYVGRKNRYGYFGVSGPLPKIK 474

Query: 501 GVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGYVVSYVHDE 560
           G+ KLD       DC V  R FG  C+ GEPFFVP   +N D     ED GYVV Y H+E
Sbjct: 475 GIRKLDFDLVGSGDCTVGRRDFGLGCFAGEPFFVP---DNID-GYGNEDSGYVVCYTHEE 534

Query: 561 NSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDL 592
           ++G+S FVVMDAKSPEL+I+A V+LP R+PYGFHG+FVK+++L
Sbjct: 535 DTGESWFVVMDAKSPELDIVAEVQLPSRIPYGFHGIFVKQAEL 572

BLAST of Carg11416 vs. ExPASy Swiss-Prot
Match: Q84K96 (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=82528 GN=ZCD PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 5.7e-120
Identity = 213/369 (57.72%), Postives = 273/369 (73.98%), Query Frame = 0

Query: 227 QFNPTNGIGLANTSLAFFGDRLYALGESDLPYPIRLTP-NGDIETLGRHDFDGKLSMSMT 286
           Q +PT GIGLANTSL F   RL+AL E DLPY +RL+P +GDI T+GR + +   + S T
Sbjct: 2   QVDPTKGIGLANTSLQFSNGRLHALCEYDLPYVVRLSPEDGDISTVGRIE-NNVSTKSTT 61

Query: 287 AHPKIDYDTGEAFAFRYGPLPPFLTFFRFDKNGAKQ-PDVRIFSMNRPSFLHDFAITKKY 346
           AHPK D  TGE F+F YGP+ P++T+ R+D +G K  PDV IFS   PSF+HDFAIT+ Y
Sbjct: 62  AHPKTDPVTGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEHY 121

Query: 347 AVFTEIQIGFNPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAI 406
           AVF +IQI   P + IV GR  +G D  K+ R+GL+PRYA  +S+M+WFDVPG N+VH +
Sbjct: 122 AVFPDIQIVMKPAE-IVRGRRMIGPDLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHVV 181

Query: 407 NAWDED--DAVILVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFG 466
           NAW+E+  + V++VAPNV  +E+ ++R DL+H  VE  RI+LK+G ++R  LS  NLDFG
Sbjct: 182 NAWEEEGGEVVVIVAPNVSPIENAIDRFDLLHVSVEMARIELKSGSVSRTLLSAENLDFG 241

Query: 467 VINPLHVGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFV 526
           VI+  + G+K+R+ Y GVGDPMPKI GVVK+D     R +C+VA R FG  C+GGEPFFV
Sbjct: 242 VIHRGYSGRKSRYAYLGVGDPMPKIRGVVKVDFELAGRGECVVARREFGVGCFGGEPFFV 301

Query: 527 PRERENSDKTAAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFH 586
           P    +S K+  EEDDGYVVSY+HDE  G+S FVVMDA+SPELEI+A V LPRRVPYGFH
Sbjct: 302 P---ASSKKSGGEEDDGYVVSYLHDEGKGESSFVVMDARSPELEILAEVVLPRRVPYGFH 361

Query: 587 GLFVKESDL 592
           GLFV E++L
Sbjct: 362 GLFVTEAEL 365

BLAST of Carg11416 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.5e-109
Identity = 228/608 (37.50%), Postives = 345/608 (56.74%), Query Frame = 0

Query: 3   AMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASR-----PDADFPSP 62
           A+S  +L G +    PP+S S+++   + CS L   +  T K   S      P   FP  
Sbjct: 9   AVSGRWLGGNH--TQPPLSSSQSS-DLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQ 68

Query: 63  SLSQRATPPPAVAKGSSTRRV---EPSVPASIFNAFDDLINNFINPPANPSTDPRYVLAD 122
           S +  A      AK S+T+++   + +  A++  A   L+++    P   + DP   +A 
Sbjct: 69  SSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAG 128

Query: 123 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 182
           NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++  +G
Sbjct: 129 NFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHG 188

Query: 183 RAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGI 242
            A    R+ +T ++  ER  G PV P      +G     AR  +   R   G  +P +G 
Sbjct: 189 SASYACRFTQTNRFVQERQLGRPVFPKAIGELHG-HTGIARLMLFYARAAAGIVDPAHGT 248

Query: 243 GLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 302
           G+AN  L +F  RL A+ E DLPY +++TPNGD++T+GR DFDG+L  +M AHPK+D ++
Sbjct: 249 GVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPES 308

Query: 303 GEAFAFRYGPL-PPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIG 362
           GE FA  Y  +  P+L +FRF  +G K PDV I  +++P+ +HDFAIT+ + V  + Q+ 
Sbjct: 309 GELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQQVV 368

Query: 363 FNPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWD--EDD 422
           F   +MI  G SPV  D +K++R G++ +YA D S +KW D P     H  NAW+  E D
Sbjct: 369 FKLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETD 428

Query: 423 AVILVAPNVLSVEHTMERMD-LVHALVEKIRIDLKTGIITRRPLSTR-----NLDFGVIN 482
            V+++   +   +      D  + +++ +IR++LKTG  TRRP+ +      NL+ G++N
Sbjct: 429 EVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVN 488

Query: 483 PLHVGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRE 542
              +G+K +F Y  + +P PK+SG  K+D++  E     V   L+G N YGGEP F+P E
Sbjct: 489 RNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFLPGE 548

Query: 543 RENSDKTAAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLF 594
                    EED+GY++ +VHDE +  S   +++A S  LE+ ATVKLP RVPYGFHG F
Sbjct: 549 -------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFHGTF 596

BLAST of Carg11416 vs. ExPASy TrEMBL
Match: A0A346DA10 (Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE=2 SV=1)

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 587/594 (98.82%), Postives = 591/594 (99.49%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFLSGGNLLLSP ISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAISSPFLSGGNLLLSPSISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQPDV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPL+VGKKNRFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSD+ AAEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDEMAAEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Carg11416 vs. ExPASy TrEMBL
Match: A0A346DA08 (Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2 SV=1)

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 580/594 (97.64%), Postives = 587/594 (98.82%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFLSGGNLLLSPP SK RTAVSTAICSVL EETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAISSPFLSGGNLLLSPPTSKYRTAVSTAICSVLTEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRAT PPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD
Sbjct: 61  QRATHPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPANGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQPDV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDE+DAVILVAPN+
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEEDAVILVAPNI 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINP++VGKKNRFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPVYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVATRLFGPN YGGEPFFVPRERE+SD+TAAEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNYYGGEPFFVPRERESSDETAAEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Carg11416 vs. ExPASy TrEMBL
Match: A0A346DA05 (Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 577/594 (97.14%), Postives = 585/594 (98.48%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFLSGGNLLLS PISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
           QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPP +PS DPRYVLADNFAPVD
Sbjct: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPV PNVFSGFNGL ASAARGAV AGR+ TGQ+NP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           +AFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF
Sbjct: 241 VAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQPDV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPN+
Sbjct: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNI 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPL+VGKKNRFVYAGV
Sbjct: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSD+TAAEEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAAEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL
Sbjct: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Carg11416 vs. ExPASy TrEMBL
Match: A0A6J1FTM6 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448011 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 5.2e-302
Identity = 507/594 (85.35%), Postives = 542/594 (91.25%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSPSLS 60
           MDA+SSPFL+GGNLLLSPPIS SR   S AI SV  EETP+TVK    R +AD PSP   
Sbjct: 1   MDALSSPFLTGGNLLLSPPISTSRPPFSMAITSVFTEETPKTVK----RTNADSPSP--- 60

Query: 61  QRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVD 120
            R  PPPA+AKG ST R+EPS+PA  FNAFDDLINNFINPP +PS DPRYVLADNFAPVD
Sbjct: 61  -RRPPPPAMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAPVD 120

Query: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180
           ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS
Sbjct: 121 ELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCS 180

Query: 181 RYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTS 240
           RYVKTYKYTLERDAGHPV PNVFSGFNGL ASAARGAV AGR+ TGQ+NP NGIGLANTS
Sbjct: 181 RYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLANTS 240

Query: 241 LAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAF 300
           +AFFGD LYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE FAF
Sbjct: 241 VAFFGDSLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETFAF 300

Query: 301 RYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 360
           RYGPLPPFLTFFRFDKNGAKQ DV IFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM MI
Sbjct: 301 RYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMLMI 360

Query: 361 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNV 420
           +EGRSPVG DPS +SR+GLIPRYA+DESKMKWFDVPG NL+HAINAWDEDDAV+L+APN+
Sbjct: 361 MEGRSPVGTDPSTVSRVGLIPRYADDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAPNI 420

Query: 421 LSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAGV 480
           LSVEHT+ERMDLVHAL+E++RIDLKTGI+TRRPLSTRNLDFGVINP ++GKKNRFVYAGV
Sbjct: 421 LSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYAGV 480

Query: 481 GDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDGY 540
           GDPMPKISGVVKLDVSQEERRDCIVA+R+FGP CYGGEPFFVPRERE+SD+TA EEDDGY
Sbjct: 481 GDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDDGY 540

Query: 541 VVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           VVSYVHDENSG+S+F+VMDAKSP+LEIIA VKLPRRV YGFHGLFVKESDL KL
Sbjct: 541 VVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVSYGFHGLFVKESDLRKL 586

BLAST of Carg11416 vs. ExPASy TrEMBL
Match: A0A5A7TP93 (Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G003220 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.8e-301
Identity = 505/595 (84.87%), Postives = 546/595 (91.76%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRPDADFPSP-SL 60
           MD++SSPFLS GNL+LSPPIS SR  +ST I SVL E+   T KK    PDAD PSP SL
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQ---TAKKNTPPPDADSPSPSSL 60

Query: 61  SQRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPV 120
            +R+ P PA+A+ SSTRRVEPS+PA +FNAFDDLINNFINPP +PS DPRY+LADNFAPV
Sbjct: 61  PRRSPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPV 120

Query: 121 DELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLC 180
           DELPPTECEVI GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLC
Sbjct: 121 DELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLC 180

Query: 181 SRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANT 240
           SRYVKTYKYTLERDAGHPV+PNVFSGFNGL ASAARGAV+ GR+ TGQ+NP NGIGLANT
Sbjct: 181 SRYVKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANT 240

Query: 241 SLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFA 300
           SLAFFGDRLYALGESDLPY IRL PNGDIETL RHDFDGKL+ SMTAHPKID DTGEAFA
Sbjct: 241 SLAFFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFA 300

Query: 301 FRYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQM 360
           FRYGPLPPFLT+FRFDKNGAKQ DV I SMNRPSFLHDFAITKKYAVF +IQIG NP QM
Sbjct: 301 FRYGPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQM 360

Query: 361 IVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPN 420
           I+EG SPVG+DPSKISR+GLIPRYANDESKMKWFDVPG+NLVHAINAWDEDDAV++VAPN
Sbjct: 361 IIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPN 420

Query: 421 VLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAG 480
           +LSVEH +ERM+LVH LVEK+RIDLKTGI+TR PLSTRNLDFGVI+P +VGKKNRFVYAG
Sbjct: 421 ILSVEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYAG 480

Query: 481 VGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAAEEDDG 540
           +GDPMPKISGV KLD+SQEERRDCIVA R+FGP CYGGEPFFVPRERE+SD+TAAEEDDG
Sbjct: 481 IGDPMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDG 540

Query: 541 YVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 595
           YVVSYVHDENSG+SRF+VMDAKSPELEIIA VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 YVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of Carg11416 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 786.2 bits (2029), Expect = 1.9e-227
Identity = 379/602 (62.96%), Postives = 473/602 (78.57%), Query Frame = 0

Query: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAI---CSVLAEETPRTVKKPASRPDADFPSP 60
           MD++SS         L   + + R++  T +    +V+ E +P  +  P+   D      
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSP--ITNPSDNNDRRNKPK 60

Query: 61  SLSQRATPPPAVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFA 120
           +L  R      +       R E ++  ++F   +D+IN FI+PP+ PS DP++VL+DNFA
Sbjct: 61  TLHNRTN--HTLVSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFA 120

Query: 121 PV-DELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRA 180
           PV DELPPT+CE+I G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I NG+A
Sbjct: 121 PVLDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKA 180

Query: 181 VLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGL 240
            LCSRYVKTYKY +E+  G PV+PNVFSGFNG+ AS ARGA+TA R+ TGQ+NP NGIGL
Sbjct: 181 TLCSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGL 240

Query: 241 ANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGE 300
           ANTSLAFF +RL+ALGESDLPY +RLT +GDIET+GR+DFDGKL+MSMTAHPK D  TGE
Sbjct: 241 ANTSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGE 300

Query: 301 AFAFRYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIG--F 360
            FAFRYGP+PPFLT+FRFD  G KQ DV IFSM  PSFLHDFAITK++A+F EIQ+G   
Sbjct: 301 TFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRM 360

Query: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDD--A 420
           N + +++EG SPVG D  K  R+G+IP+YA DES+MKWF+VPG N++HAINAWDEDD  +
Sbjct: 361 NMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNS 420

Query: 421 VILVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKK 480
           V+L+APN++S+EHT+ERMDLVHALVEK++IDL TGI+ R P+S RNLDF VINP  +G+ 
Sbjct: 421 VVLIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRC 480

Query: 481 NRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKT 540
           +R+VYA +GDPMPKISGVVKLDVS+ +R DC VA R++G  CYGGEPFFV R+  N +  
Sbjct: 481 SRYVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARDPGNPE-- 540

Query: 541 AAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLS 595
            AEEDDGYVV+YVHDE +G+S+F+VMDAKSPELEI+A V+LPRRVPYGFHGLFVKESDL+
Sbjct: 541 -AEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLN 595

BLAST of Carg11416 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 398.3 bits (1022), Expect = 1.1e-110
Identity = 228/608 (37.50%), Postives = 345/608 (56.74%), Query Frame = 0

Query: 3   AMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASR-----PDADFPSP 62
           A+S  +L G +    PP+S S+++   + CS L   +  T K   S      P   FP  
Sbjct: 9   AVSGRWLGGNH--TQPPLSSSQSS-DLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQ 68

Query: 63  SLSQRATPPPAVAKGSSTRRV---EPSVPASIFNAFDDLINNFINPPANPSTDPRYVLAD 122
           S +  A      AK S+T+++   + +  A++  A   L+++    P   + DP   +A 
Sbjct: 69  SSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAG 128

Query: 123 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 182
           NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++  +G
Sbjct: 129 NFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHG 188

Query: 183 RAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGI 242
            A    R+ +T ++  ER  G PV P      +G     AR  +   R   G  +P +G 
Sbjct: 189 SASYACRFTQTNRFVQERQLGRPVFPKAIGELHG-HTGIARLMLFYARAAAGIVDPAHGT 248

Query: 243 GLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 302
           G+AN  L +F  RL A+ E DLPY +++TPNGD++T+GR DFDG+L  +M AHPK+D ++
Sbjct: 249 GVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPES 308

Query: 303 GEAFAFRYGPL-PPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIG 362
           GE FA  Y  +  P+L +FRF  +G K PDV I  +++P+ +HDFAIT+ + V  + Q+ 
Sbjct: 309 GELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQQVV 368

Query: 363 FNPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWD--EDD 422
           F   +MI  G SPV  D +K++R G++ +YA D S +KW D P     H  NAW+  E D
Sbjct: 369 FKLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETD 428

Query: 423 AVILVAPNVLSVEHTMERMD-LVHALVEKIRIDLKTGIITRRPLSTR-----NLDFGVIN 482
            V+++   +   +      D  + +++ +IR++LKTG  TRRP+ +      NL+ G++N
Sbjct: 429 EVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVN 488

Query: 483 PLHVGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRE 542
              +G+K +F Y  + +P PK+SG  K+D++  E     V   L+G N YGGEP F+P E
Sbjct: 489 RNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFLPGE 548

Query: 543 RENSDKTAAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLF 594
                    EED+GY++ +VHDE +  S   +++A S  LE+ ATVKLP RVPYGFHG F
Sbjct: 549 -------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFHGTF 596

BLAST of Carg11416 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 388.3 bits (996), Expect = 1.1e-107
Identity = 211/538 (39.22%), Postives = 313/538 (58.18%), Query Frame = 0

Query: 64  TPPPAVAKGSSTR--RVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPVDE 123
           +P P + +  ++R   ++ +  A++  A   L+    + P   + DPR+ ++ N+APV E
Sbjct: 63  SPNPIIPEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPE 122

Query: 124 LPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSR 183
                   + G +P  ++G Y+RNG NP + P   +HLFDGDGM+H+++I+NG A    R
Sbjct: 123 QSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCR 182

Query: 184 YVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTSL 243
           + +T +   E+  G P+ P      +G  +  AR  +   R   G  N  NG G+AN  L
Sbjct: 183 FTETERLVQEKQLGSPIFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAGL 242

Query: 244 AFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAFR 303
            +F DRL A+ E DLPY +R+T NGD+ET+GR DFDG+LS +M AHPKID  T E FA  
Sbjct: 243 VYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALS 302

Query: 304 YGPL-PPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMI 363
           Y  +  P+L +F+F   G K PDV I  +  P+ +HDFAIT+ + V  + Q+ F    M 
Sbjct: 303 YDVVKKPYLKYFKFSPEGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMF 362

Query: 364 VEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWD--EDDAVILVAP 423
           + G+SPV  D  KISR G++PR A D S+M W + P     H  NAW+  E D V+++  
Sbjct: 363 L-GKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGS 422

Query: 424 NVLSVEHTMERMD-LVHALVEKIRIDLKTGIITRRPL----STRNLDFGVINPLHVGKKN 483
            +   +      D  +++++ +IR++LKTG  TRR +       NL+ G++N   +G+K 
Sbjct: 423 CMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKT 482

Query: 484 RFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTA 543
           R+ Y  + +P PK+SG  K+D+S  E     V    +G   YGGEPFF+PR  E+     
Sbjct: 483 RYAYLAIAEPWPKVSGFAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG--- 542

Query: 544 AEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDL 592
             EDDGY++S+VHDE S +S   +++A + ELE  ATVKLP RVPYGFHG FV  +D+
Sbjct: 543 --EDDGYIMSFVHDEESWESELHIVNAVTLELE--ATVKLPSRVPYGFHGTFVNSADM 585

BLAST of Carg11416 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 372.1 bits (954), Expect = 8.4e-103
Identity = 203/542 (37.45%), Postives = 308/542 (56.83%), Query Frame = 0

Query: 69  VAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLADNFAPV-DELPPTEC 128
           ++ GSS   V P       +   DL+   +    + ++ P + L+ NFAP+ DE PP + 
Sbjct: 5   LSDGSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKD 64

Query: 129 EVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRYVKTYK 188
             + G LP  LNG ++R GPNP++     YH FDGDGM+H +RI +G+A   SRYVKT +
Sbjct: 65  LPVHGFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSR 124

Query: 189 YTLERDAGHPVIPNV--FSGFNGLIASAARGAVTAGRMFTGQFNPTNGIGLANTSLAFFG 248
              E   G      +    GF GL+    +   T  ++     + T G G ANT+L +  
Sbjct: 125 LKQEEFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKI----LDNTYGNGTANTALVYHH 184

Query: 249 DRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDTGEAFAFRYGPL 308
            +L AL E+D PY I++  +GD++TLG  D+D +L+ S TAHPK+D  TGE F F Y   
Sbjct: 185 GKLLALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHT 244

Query: 309 PPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPMQMIVEGRS 368
           PP+LT+    K+G     V I +++ P  +HDFAIT+ YA+F ++ + F P +M+ E + 
Sbjct: 245 PPYLTYRVISKDGIMHDPVPI-TISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKM 304

Query: 369 PVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVILVAPNVLSVEH 428
               DP+K +R G++PRYA DE  ++WF++P   + H  NAW+E+D V+L+   + + + 
Sbjct: 305 IYSFDPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDL 364

Query: 429 TM------ERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNRFVYAG 488
            M      E+++     + ++R ++KTG  +++ LS   +DF  IN  + GKK R+VY  
Sbjct: 365 DMVSGKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGT 424

Query: 489 VGDPMPKISGVVKLDVSQEE---RRDCIVATRL-----FGPNCYGGEPFFVPRERENSDK 548
           + D + K++G++K D+  E    +R   V   +      G   YG E  +VPRE      
Sbjct: 425 ILDSIAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRE------ 484

Query: 549 TAAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDL 594
             AEEDDGY++ +VHDEN+G S   V+DAK+   E +A V+LP RVPYGFH LFV E  L
Sbjct: 485 -TAEEDDGYLIFFVHDENTGKSCVTVIDAKTMSAEPVAVVELPHRVPYGFHALFVTEEQL 534

BLAST of Carg11416 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 371.7 bits (953), Expect = 1.1e-102
Identity = 198/503 (39.36%), Postives = 290/503 (57.65%), Query Frame = 0

Query: 101 PANPSTDPRYVLADNFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLF 160
           P   + DP   +A NF PV E P      + G++P  + G Y+RNG NP + P   +HLF
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLF 231

Query: 161 DGDGMLHSLRISNGRAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTA 220
           DGDGM+H++R  NG      R+ +T +   ER+ G PV P      +G +  A       
Sbjct: 232 DGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNT 291

Query: 221 GRMFTGQFNPTNGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKL 280
             +F G  +PT G+G+AN  L +F   L A+ E DLPY +++T  GD+ET GR+DFDG+L
Sbjct: 292 RGLF-GLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQL 351

Query: 281 SMSMTAHPKIDYDTGEAFAFRYGPL-PPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFA 340
             +M AHPKID +T E FA  Y  +  P+L +FRF  +G K PDV I  +++P+ +HDFA
Sbjct: 352 KSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEI-PLDQPTMIHDFA 411

Query: 341 ITKKYAVFTEIQIGFNPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVN 400
           IT+ + V  + Q+ F   +MI  G SPV  D  K SR G++ + A D S ++W +VP   
Sbjct: 412 ITENFVVIPDQQVVFRLPEMI-RGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCF 471

Query: 401 LVHAINAWDE---DDAVI----LVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRR 460
             H  N+W+E   D+ V+    +  P+ +  EH     + + +++ +IR++LKTG  TRR
Sbjct: 472 CFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHD----ETLQSVLSEIRLNLKTGESTRR 531

Query: 461 PLSTR--NLDFGVINPLHVGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLF 520
           P+ +   NL+ G++N   +G+K R+ Y  + +P PK+SG  K+D+S  E R  I     +
Sbjct: 532 PVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYI-----Y 591

Query: 521 GPNCYGGEPFFVPRERENSDKTAAEEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIAT 580
           G   YGGEP F+P           EED GY++ +VHDE    S   +++A + +LE  AT
Sbjct: 592 GEGKYGGEPLFLP-------SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLE--AT 651

Query: 581 VKLPRRVPYGFHGLFVKESDLSK 594
           V LP RVPYGFHG F+ + DLSK
Sbjct: 652 VTLPSRVPYGFHGTFISKEDLSK 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015104.10.0e+00100.00putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita ar... [more]
XP_022931473.10.0e+0098.82probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata] >... [more]
XP_022985089.10.0e+0097.64probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita maxima] >AX... [more]
XP_023553272.10.0e+0097.82LOW QUALITY PROTEIN: probable carotenoid cleavage dioxygenase 4, chloroplastic [... [more]
AXM43896.10.0e+0097.14carotenoid cleavage dioxygenase [Cucurbita pepo][more]
Match NameE-valueIdentityDescription
O496752.6e-22662.96Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thalian... [more]
Q6YVJ02.9e-19357.879-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q5MBR65.1e-16154.689-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q84K965.7e-12057.72Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=8... [more]
Q9LRR71.5e-10937.509-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A346DA100.0e+0098.82Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE... [more]
A0A346DA080.0e+0097.64Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2... [more]
A0A346DA050.0e+0097.14Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1FTM65.2e-30285.35probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata ... [more]
A0A5A7TP935.8e-30184.87Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
AT4G19170.11.9e-22762.96nine-cis-epoxycarotenoid dioxygenase 4 [more]
AT3G14440.11.1e-11037.50nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.11.1e-10739.22nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT3G63520.18.4e-10337.45carotenoid cleavage dioxygenase 1 [more]
AT1G78390.11.1e-10239.36nine-cis-epoxycarotenoid dioxygenase 9 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 117..586
e-value: 1.0E-107
score: 361.2
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 67..593
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..77
NoneNo IPR availablePANTHERPTHR10543:SF46CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATEDcoord: 67..593

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11416-RACarg11416-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen