Carg11350 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11350
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Malate dehydrogenase
Location	Carg_Chr16: 2359564 .. 2362821 (-)
RNA-Seq Expression	Carg11350
Synteny	Carg11350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAACCCTTCATTTCCGCAAAACCCTTTCTTCTTCTTCTTCTTCTTCTTCTCTTTATCGGTTTCGTCGGTGTTCTTCTACGTGGAACAATGAAGGCCTCACTTCTCAGATCCGTTCGTTCTGCCGTTTCAAGGTCCTCATCGTCGAATCACCTCTTGAGACGTAGCTTTGGGACCGAAGCGGTGCCGGAAAGGAAAGTCACCGTACTCGGTGCCGCTGGTGGGATTGGGCAGCCATTGTCGCTTCTTATGAAGCTCAATCCACTTGTTTCTAGGCTCGCACTCTACGATATCGCTGGTACTCCTGGTGTTGCCGCCGATGTCGGTCATGTTAACACTAGATCCGAGGTCAATATCGTTTCTTTCCACCTTTTTTCTTGCACGGTTACGAGTAATTGTGTTTTGATTTGTGGATGCCTCTTTTAGGAATGCAATGGATCTTGTTTTCGTTCGCGTGATTTCAATTTCTGTGTTATTTTGTTTCCGGATGTATATTGTTTGTTTTCTGCTCCATATTTTAAGTCGATTTTGAGTTATGTTTTGTTCTAATGCGTTTCGTGTTCATCGATTTGGGCTATTTTTGTTCATTTCATGATTTTTTGCATTCTCTGTAATTTATTTAAATCAGTGTTCTTTGCATGCTTATTCTAAACTCTTTTTGCGACTTGTAGGTTACCGGCTACGCTGGTGAGGAGCAACTCGCCAAAGCTTTGGAGGGATCTGATGTTGTCATCATTCCTGCCGGTGTTCCGAGAAAGCCTGGGATGACCCGTGATGATCTCTTCAACATCAATGCTGGCATTGTTAAATCACTTTGCACAGCCATTGCTAAGTACTGCCCCAATGTCTGTTCCCACGACTCTTCTACTGATGCATTTTCTTTTCTTTAGTTTCTTTTTTATCTGGCTTCATTTTGTAATCGAAATACGGATTTGCAGGCTCTTGTTAACATGATCAGTAACCCCGTCAACTCAACTGTCCCAATTGCTGCTGAGGTTTTCAAGAAAGCTGGTACTTACGATGAAAGGAAGTTGTTTGGTGTTACTACTCTTGATGTCGTCAGGGCCAAGACCTTTTATGCTGGGAAAGCTAATGCTCCAGTATCTGGTATAAGTCGTTGCTATAATTCCGTTTTTGAAATTTCTCTGTAATTTTCCATTTACAATGATCAAGGATGTTTCCTTGTTACGCTGCACACATAGGAGCAAGGTCAAACTTTATTGCCTGACTGTTGTAATACTGTTACAGAGGTTAATGTGCCTGTTATCGGTGGCCATGCTGGTATTACTATTCTTCCACTATTTTCTCAGGTACTTCCCTTTCTAAGTCAGCTTTCTTTCTGTTTACAATTTTATTAGTGATATAAGCAGTGTTCTTGCATCGGATGCTTTGGATTAGAGGGCCTTGTTGTTTTTATACAGTGTATCTGATTGTCACAGGCTACCCCAAAAGCTAATTTGTCAGATGATACTATTTTGGCTCTCACCAAGCGAACTCAAGATGGAGGAACAGAAGTTGTGGAAGCAAAGGCAGGAAAGGGTTCTGCTACATTATCAATGGCGTAAGACTTTTAACTTTCTGCTGGATTATTTATTAGAAGTTTATTTCCTGAACTTCGAGGCTTGTGAACAGTATTGTTATGTTGCGATCATCACACAAACATTACTCGGTGAACAATGTGAATATATTAAACGTTTTTGAATTACTGTTTCTGGCAGCTATGCTGGAGCCCTTTTCGCCGATGCTTGTCTCAAGGGATTGAATGGGGTTCCAGATGTTGTGGAATGCACGTTTGTGCAATCAACTGTCACTGAACTTCCATTCTTTGCCTCAAAGGTAACTGGTTCCGTCACTGCTTTTTCCTTATAGCATATATTTGAGATGAAACTCGAAAAATTAGAAGTTGAACTGTTGGTGTTTCTTTATAGTCGCAATCCTCCAACAATATGAATTGTTGTTCACTTTGATTTTATTGTATAAAACAGGTTAAGCTTGGAAAGAATGGAGTAGAACAAGTTTTGGATTTGGGTACTCTCTCAGACTTCGAGAAAGAAGGTTTGGAAAAGCTCAAGCCCGAGCTCAAAGCTTCTATTGAGAAAGGAATCCAGTTTGCAAACGCCAATTAGAAGATGTTTATATTATTTACAATCAGGACAGGTATCTCTGCCCGCATCACCATAAAACTCATCAATTTTTGCTTTCCCCGACCCAATTGTAACTGATAATGCTGCTTACGAGCAACCGGCTTTACTGTTTTTGTTAGGTTAGATCATTTTCTGTCGTATAAATTTTATCAATAATTCCCCGTGGCATGTTTTTGTGGGATTTCTGGTTGGTTACTTCTGAACCTGTGATACTTCATATTAGACTCTTCAATTCTTAATATTGAGAATCGTCCCACCATGATAGGTTGTGGAAAAGTTTCCTTCCATTGATTTAGTGTTCAGTGGATTTCATCAGATTTCTAAATGTTTTAAAATAAAAAGTGCTTTTAAATGCAACAAAAAGATGTTCTTTCTTTTGTTCTGTTGGTCACAAACTGTTTATATATCAATTTTGCGTGGAGGTTGAATTTTTTAAAATTTGTGTTCATATTGGTGGTGGATTGTGTGTTCAGAATTTTAGTCATTGATAAATTTTCACAAACCCAATTGGTATATTGATATGACAGGGCAACCCAATATTTCATGATATTAGTTTAGATGATATTAGTTTTGTGTTCTTATATATATATATATATATTATTTTTTTGAATAATTATTTTTTGACTGTTAATTCAGACCTTTGAAAAGACGTTCTGTTATTGATGGATCATCCTAAGAACTTTTTATTTGAATGAGATTGAATGATAAAATGAAATTAAGTTGCCTCAATAATAACTAAGAATACTTTGATTTTCTGTTTTTGAAAGTTGAACCAAATCGAGTTCTTGAAAACTATTAAAAAATCATTTTTTTTAGAAGAAATATTTTTGTATTGAATGTCACTAAGAATTCAAGTATGAATGAAACTCTAGTACAAAATTTGGGATGAAACGCGTTAGTTGTGAAAACAAAAAAACTAAAATTAAAATCATTATCAAGAGAGGATAGTGTTTAAAAGAATAGATGGCACCGAGAAATGTTTACCTAAGTCTCACTGGAGATGTCTACTTTGTGTTACATCAACATAATAATCCTCGTATTTACAATATTAACATTTTTTTAAAGCTTCTATCCTCATGGAGTCAGATTTACACAAATTATATGAAAGTCAGCATA

mRNA sequence

ATAACCCTTCATTTCCGCAAAACCCTTTCTTCTTCTTCTTCTTCTTCTTCTCTTTATCGGTTTCGTCGGTGTTCTTCTACGTGGAACAATGAAGGCCTCACTTCTCAGATCCGTTCGTTCTGCCGTTTCAAGGTCCTCATCGTCGAATCACCTCTTGAGACGTAGCTTTGGGACCGAAGCGGTGCCGGAAAGGAAAGTCACCGTACTCGGTGCCGCTGGTGGGATTGGGCAGCCATTGTCGCTTCTTATGAAGCTCAATCCACTTGTTTCTAGGCTCGCACTCTACGATATCGCTGGTACTCCTGGTGTTGCCGCCGATGTCGGTCATGTTAACACTAGATCCGAGGTTACCGGCTACGCTGGTGAGGAGCAACTCGCCAAAGCTTTGGAGGGATCTGATGTTGTCATCATTCCTGCCGGTGTTCCGAGAAAGCCTGGGATGACCCGTGATGATCTCTTCAACATCAATGCTGGCATTGTTAAATCACTTTGCACAGCCATTGCTAAGTACTGCCCCAATGCTCTTGTTAACATGATCAGTAACCCCGTCAACTCAACTGTCCCAATTGCTGCTGAGGTTTTCAAGAAAGCTGGTACTTACGATGAAAGGAAGTTGTTTGGTGTTACTACTCTTGATGTCGTCAGGGCCAAGACCTTTTATGCTGGGAAAGCTAATGCTCCAGTATCTGGTATAAGTCGTTGCTATAATTCCGAGCAAGGTCAAACTTTATTGCCTGACTGTTGTAATACTGTTACAGAGGTTAATGTGCCTGTTATCGGTGGCCATGCTGGTATTACTATTCTTCCACTATTTTCTCAGTGTTCTTGCATCGGATGCTTTGGATTAGAGGGCCTTGTTGCTACCCCAAAAGCTAATTTGTCAGATGATACTATTTTGGCTCTCACCAAGCGAACTCAAGATGGAGGAACAGAAGTTGTGGAAGCAAAGGCAGGAAAGGGTTCTGCTACATTATCAATGGCCTATGCTGGAGCCCTTTTCGCCGATGCTTGTCTCAAGGGATTGAATGGGGTTCCAGATGTTGTGGAATGCACGTTTGTGCAATCAACTGTCACTGAACTTCCATTCTTTGCCTCAAAGGTTAAGCTTGGAAAGAATGGAGTAGAACAAGTTTTGGATTTGGGTACTCTCTCAGACTTCGAGAAAGAAGGTTTGGAAAAGCTCAAGCCCGAGCTCAAAGCTTCTATTGAGAAAGGAATCCAGTTTGCAAACGCCAATTAGAAGATGTTTATATTATTTACAATCAGGACAGGTATCTCTGCCCGCATCACCATAAAACTCATCAATTTTTGCTTTCCCCGACCCAATTGTAACTGATAATGCTGCTTACGAGCAACCGGCTTTACTGTTTTTGTTAGGTTAGATCATTTTCTGTCGTATAAATTTTATCAATAATTCCCCGTGGCATGTTTTTGTGGGATTTCTGGTTGGTTACTTCTGAACCTGTGATACTTCATATTAGACTCTTCAATTCTTAATATTGAGAATCGTCCCACCATGATAGGTTGTGGAAAAGTTTCCTTCCATTGATTTAGTGTTCAGTGGATTTCATCAGATTTCTAAATGTTTTAAAATAAAAAGTGCTTTTAAATGCAACAAAAAGATGTTCTTTCTTTTGTTCTGTTGGTCACAAACTGTTTATATATCAATTTTGCGTGGAGGTTGAATTTTTTAAAATTTGTGTTCATATTGGTGGTGGATTGTGTGTTCAGAATTTTAGTCATTGATAAATTTTCACAAACCCAATTGGTATATTGATATGACAGGGCAACCCAATATTTCATGATATTAGTTTAGATGATATTAGTTTTGTGTTCTTATATATATATATATATATTATTTTTTTGAATAATTATTTTTTGACTGTTAATTCAGACCTTTGAAAAGACGTTCTGTTATTGATGGATCATCCTAAGAACTTTTTATTTGAATGAGATTGAATGATAAAATGAAATTAAGTTGCCTCAATAATAACTAAGAATACTTTGATTTTCTGTTTTTGAAAGTTGAACCAAATCGAGTTCTTGAAAACTATTAAAAAATCATTTTTTTTAGAAGAAATATTTTTGTATTGAATGTCACTAAGAATTCAAGTATGAATGAAACTCTAGTACAAAATTTGGGATGAAACGCGTTAGTTGTGAAAACAAAAAAACTAAAATTAAAATCATTATCAAGAGAGGATAGTGTTTAAAAGAATAGATGGCACCGAGAAATGTTTACCTAAGTCTCACTGGAGATGTCTACTTTGTGTTACATCAACATAATAATCCTCGTATTTACAATATTAACATTTTTTTAAAGCTTCTATCCTCATGGAGTCAGATTTACACAAATTATATGAAAGTCAGCATA

Coding sequence (CDS)

ATGAAGGCCTCACTTCTCAGATCCGTTCGTTCTGCCGTTTCAAGGTCCTCATCGTCGAATCACCTCTTGAGACGTAGCTTTGGGACCGAAGCGGTGCCGGAAAGGAAAGTCACCGTACTCGGTGCCGCTGGTGGGATTGGGCAGCCATTGTCGCTTCTTATGAAGCTCAATCCACTTGTTTCTAGGCTCGCACTCTACGATATCGCTGGTACTCCTGGTGTTGCCGCCGATGTCGGTCATGTTAACACTAGATCCGAGGTTACCGGCTACGCTGGTGAGGAGCAACTCGCCAAAGCTTTGGAGGGATCTGATGTTGTCATCATTCCTGCCGGTGTTCCGAGAAAGCCTGGGATGACCCGTGATGATCTCTTCAACATCAATGCTGGCATTGTTAAATCACTTTGCACAGCCATTGCTAAGTACTGCCCCAATGCTCTTGTTAACATGATCAGTAACCCCGTCAACTCAACTGTCCCAATTGCTGCTGAGGTTTTCAAGAAAGCTGGTACTTACGATGAAAGGAAGTTGTTTGGTGTTACTACTCTTGATGTCGTCAGGGCCAAGACCTTTTATGCTGGGAAAGCTAATGCTCCAGTATCTGGTATAAGTCGTTGCTATAATTCCGAGCAAGGTCAAACTTTATTGCCTGACTGTTGTAATACTGTTACAGAGGTTAATGTGCCTGTTATCGGTGGCCATGCTGGTATTACTATTCTTCCACTATTTTCTCAGTGTTCTTGCATCGGATGCTTTGGATTAGAGGGCCTTGTTGCTACCCCAAAAGCTAATTTGTCAGATGATACTATTTTGGCTCTCACCAAGCGAACTCAAGATGGAGGAACAGAAGTTGTGGAAGCAAAGGCAGGAAAGGGTTCTGCTACATTATCAATGGCCTATGCTGGAGCCCTTTTCGCCGATGCTTGTCTCAAGGGATTGAATGGGGTTCCAGATGTTGTGGAATGCACGTTTGTGCAATCAACTGTCACTGAACTTCCATTCTTTGCCTCAAAGGTTAAGCTTGGAAAGAATGGAGTAGAACAAGTTTTGGATTTGGGTACTCTCTCAGACTTCGAGAAAGAAGGTTTGGAAAAGCTCAAGCCCGAGCTCAAAGCTTCTATTGAGAAAGGAATCCAGTTTGCAAACGCCAATTAG

Protein sequence

MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKPELKASIEKGIQFANAN

Homology

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BLAST of Carg11350 vs. NCBI nr
Match: KAG7015040.1 (Malate dehydrogenase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 746.1 bits (1925), Expect = 1.5e-211
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV
Sbjct: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 383

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BLAST of Carg11350 vs. NCBI nr
Match: XP_022984474.1 (malate dehydrogenase, mitochondrial [Cucurbita maxima] >XP_023552175.1 malate dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo] >KAG6577018.1 Malate dehydrogenase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 643.7 bits (1659), Expect = 1.0e-180
Identity = 346/383 (90.34%), Postives = 347/383 (90.60%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKASLLRSVRSAVSRSSSSNHLLRRSFGTE+VPERKVTVLGAAGGIGQPLSLLMKLNPLV
Sbjct: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTESVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKANAPVS                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANAPVS-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

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BLAST of Carg11350 vs. NCBI nr
Match: XP_022922965.1 (malate dehydrogenase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 642.5 bits (1656), Expect = 2.3e-180
Identity = 345/383 (90.08%), Postives = 347/383 (90.60%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKASLLRSVRSAVSRSS+SNHLLRRSFGTE+VPERKVTVLGAAGGIGQPLSLLMKLNPLV
Sbjct: 1   MKASLLRSVRSAVSRSSASNHLLRRSFGTESVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKANAPVS                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANAPVS-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

[Close]

BLAST of Carg11350 vs. NCBI nr
Match: XP_038878007.1 (malate dehydrogenase, mitochondrial [Benincasa hispida])

HSP 1 Score: 619.4 bits (1596), Expect = 2.1e-173
Identity = 330/383 (86.16%), Postives = 340/383 (88.77%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKAS+LRSVRSAVSRSSSSNHLL RSF TE+VPERKV VLGAAGGIGQPL+LLMKLNPLV
Sbjct: 1   MKASILRSVRSAVSRSSSSNHLLSRSFATESVPERKVAVLGAAGGIGQPLALLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           S+LALYDIAGTPGVAADVGHVNTRSEV GYAGEEQL KALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SKLALYDIAGTPGVAADVGHVNTRSEVAGYAGEEQLGKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDE+KLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDEKKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKANAPV+                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANAPVA-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANLSD+TI+ALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLSDETIVALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVEC+FVQST+TELPFFASKVKLGKNGVE VLDLG LSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECSFVQSTITELPFFASKVKLGKNGVESVLDLGPLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

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BLAST of Carg11350 vs. NCBI nr
Match: P17783.1 (RecName: Full=Malate dehydrogenase, mitochondrial; AltName: Allergen=Citr l MDH; Flags: Precursor [Citrullus lanatus] >CAA35239.1 unnamed protein product [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 615.5 bits (1586), Expect = 3.0e-172
Identity = 328/383 (85.64%), Postives = 338/383 (88.25%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKAS+LRSVRSAVSRSSSSN LL RSF TE+VPERKV VLGAAGGIGQPL+LLMKLNPLV
Sbjct: 1   MKASILRSVRSAVSRSSSSNRLLSRSFATESVPERKVAVLGAAGGIGQPLALLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           S+LALYDIAGTPGVAADVGHVNTRSEVTGY GEEQL KALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SKLALYDIAGTPGVAADVGHVNTRSEVTGYVGEEQLGKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNAL+NMISNPVNSTVPIAAEVFKKAGTYDE+KLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALINMISNPVNSTVPIAAEVFKKAGTYDEKKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKAN PV+                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANVPVA-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATP+ANLSDDTI+ALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPRANLSDDTIVALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVEC+FVQSTVTELPFFASKVKLGKNGVE VLDLG LSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECSFVQSTVTELPFFASKVKLGKNGVESVLDLGPLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

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BLAST of Carg11350 vs. ExPASy Swiss-Prot
Match: P17783 (Malate dehydrogenase, mitochondrial OS=Citrullus lanatus OX=3654 GN=MMDH PE=1 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 4.0e-175
Identity = 328/383 (85.64%), Postives = 338/383 (88.25%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKAS+LRSVRSAVSRSSSSN LL RSF TE+VPERKV VLGAAGGIGQPL+LLMKLNPLV
Sbjct: 1   MKASILRSVRSAVSRSSSSNRLLSRSFATESVPERKVAVLGAAGGIGQPLALLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           S+LALYDIAGTPGVAADVGHVNTRSEVTGY GEEQL KALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SKLALYDIAGTPGVAADVGHVNTRSEVTGYVGEEQLGKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNAL+NMISNPVNSTVPIAAEVFKKAGTYDE+KLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALINMISNPVNSTVPIAAEVFKKAGTYDEKKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKAN PV+                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANVPVA-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATP+ANLSDDTI+ALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPRANLSDDTIVALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVEC+FVQSTVTELPFFASKVKLGKNGVE VLDLG LSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECSFVQSTVTELPFFASKVKLGKNGVESVLDLGPLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

[Close]

BLAST of Carg11350 vs. ExPASy Swiss-Prot
Match: P46487 (Malate dehydrogenase, mitochondrial OS=Eucalyptus gunnii OX=3933 GN=MDH PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 7.5e-158
Identity = 295/382 (77.23%), Postives = 324/382 (84.82%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           M+AS+LR +RS  S ++   HLLRR++G+E+VPERKV VLGAAGGIGQPL+LLMKLNPLV
Sbjct: 1   MRASMLRLIRSRSSSAAPRPHLLRRAYGSESVPERKVAVLGAAGGIGQPLALLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           S+LALYDIAGTPGVAADVGH+NTRSEV GY GEEQL +ALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SQLALYDIAGTPGVAADVGHINTRSEVAGYVGEEQLGQALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNA+VNMISNPVNSTVPIAAE+FKKAGTY+E+KL GVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNAVVNMISNPVNSTVPIAAEIFKKAGTYNEKKLLGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKA  PV                        EVNVPV+GGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKAKVPVE-----------------------EVNVPVVGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             A PKANL+D+ I ALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------AVPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQST-VTELPFFASKVKLGKNGVEQVLDLGTLSDFEK 360
           GALFADACLKGLNGVPDVVEC+FVQS+ +TELPFFASKVKLGKNGVE+VL+LG +SD+EK
Sbjct: 301 GALFADACLKGLNGVPDVVECSFVQSSIITELPFFASKVKLGKNGVEEVLELGPMSDYEK 346

Query: 361 EGLEKLKPELKASIEKGIQFAN 382
           +GLE L PELKASIEKGI+FAN
Sbjct: 361 QGLEILIPELKASIEKGIKFAN 346

[Close]

BLAST of Carg11350 vs. ExPASy Swiss-Prot
Match: Q9ZP06 (Malate dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g53240 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 3.9e-154
Identity = 284/374 (75.94%), Postives = 317/374 (84.76%), Query Frame = 0


Query: 10  RSAVSRSSSS--NHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYD 69
           RS + RSS+S    ++RRSF + +VPERKV +LGAAGGIGQPL+LLMKLNPLVS L+LYD
Sbjct: 3   RSMLVRSSASAKQAVIRRSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYD 62

Query: 70  IAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNIN 129
           IA TPGVAADVGH+NTRSEV GY G++ LAKALEG+D+VIIPAGVPRKPGMTRDDLFNIN
Sbjct: 63  IANTPGVAADVGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNIN 122

Query: 130 AGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRA 189
           AGIVK+LCTAIAKYCP+AL+NMISNPVNSTVPIAAE+FKKAG YDE+KLFGVTTLDVVRA
Sbjct: 123 AGIVKNLCTAIAKYCPHALINMISNPVNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRA 182

Query: 190 KTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSC 249
           +TFYAGKAN PV+                       EVNVPVIGGHAG+TILPLFSQ   
Sbjct: 183 RTFYAGKANVPVA-----------------------EVNVPVIGGHAGVTILPLFSQ--- 242

Query: 250 IGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 309
                     ATP+ANLS D + ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA
Sbjct: 243 ----------ATPQANLSSDILTALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 302

Query: 310 CLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKP 369
           CLKGLNGVPDV+EC++VQST+TELPFFASKV+LGKNGVE+VLDLG LSDFEKEGLE LKP
Sbjct: 303 CLKGLNGVPDVIECSYVQSTITELPFFASKVRLGKNGVEEVLDLGPLSDFEKEGLEALKP 340

Query: 370 ELKASIEKGIQFAN 382
           ELK+SIEKG++FAN
Sbjct: 363 ELKSSIEKGVKFAN 340

[Close]

BLAST of Carg11350 vs. ExPASy Swiss-Prot
Match: P83373 (Malate dehydrogenase, mitochondrial OS=Fragaria ananassa OX=3747 GN=MMDHI PE=1 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 1.8e-151
Identity = 281/373 (75.34%), Postives = 313/373 (83.91%), Query Frame = 0


Query: 9   VRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYDI 68
           +R ++S   S + + RR + +E+VP+RKV VLGAAGGIGQPL+LLMKLNPLVS+L+LYDI
Sbjct: 1   MRPSMSLIRSVSRVARRGYSSESVPQRKVAVLGAAGGIGQPLALLMKLNPLVSQLSLYDI 60

Query: 69  AGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNINA 128
           AGTPGVAADV H+NTRSEV GYAGEEQL +ALEG DVVIIPAGVPRKPGMTRDDLFNINA
Sbjct: 61  AGTPGVAADVSHINTRSEVKGYAGEEQLGEALEGCDVVIIPAGVPRKPGMTRDDLFNINA 120

Query: 129 GIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRAK 188
           GIV+SL  AIAKYCP+A++NMISNPVNSTVPIA+EV KKAG YDE+KLFGVTTLDVVRAK
Sbjct: 121 GIVRSLTAAIAKYCPHAIINMISNPVNSTVPIASEVLKKAGVYDEKKLFGVTTLDVVRAK 180

Query: 189 TFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSCI 248
           TFYAGKA  PV+                       EVNVPV+GGHAGITILPLFSQ    
Sbjct: 181 TFYAGKAGVPVA-----------------------EVNVPVVGGHAGITILPLFSQ---- 240

Query: 249 GCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADAC 308
                    ATPKANLSDD I ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADAC
Sbjct: 241 ---------ATPKANLSDDYIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADAC 300

Query: 309 LKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKPE 368
           L GLNGVPDVVEC++VQS++TELPFFASKV+LGKNGVE+VLDLG LSDFEKEGL++LKPE
Sbjct: 301 LXGLNGVPDVVECSYVQSSITELPFFASKVRLGKNGVEEVLDLGPLSDFEKEGLKQLKPE 337

Query: 369 LKASIEKGIQFAN 382
           LK+SIEKGI+FAN
Sbjct: 361 LKSSIEKGIKFAN 337

[Close]

BLAST of Carg11350 vs. ExPASy Swiss-Prot
Match: Q9LKA3 (Malate dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g15020 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.0e-150
Identity = 282/374 (75.40%), Postives = 312/374 (83.42%), Query Frame = 0


Query: 10  RSAVSRSSS--SNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYD 69
           RS + RS+S     LLRR F +E+VP+RKV +LGAAGGIGQPLSLLMKLNPLVS L+LYD
Sbjct: 3   RSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSLYD 62

Query: 70  IAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNIN 129
           IA TPGVAADVGH+NTRS+V+GY G++ L KALEG+D+VIIPAGVPRKPGMTRDDLFNIN
Sbjct: 63  IANTPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLFNIN 122

Query: 130 AGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRA 189
           AGIVK+L  AIAKYCP ALVNMISNPVNSTVPIAAE+FKKAGTYDE+KLFGVTTLDVVRA
Sbjct: 123 AGIVKNLSIAIAKYCPQALVNMISNPVNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVVRA 182

Query: 190 KTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSC 249
           +TFYAGK++                         V EVNVPV+GGHAGITILPLFSQ   
Sbjct: 183 RTFYAGKSDV-----------------------NVAEVNVPVVGGHAGITILPLFSQ--- 242

Query: 250 IGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 309
                     A+P+ANLSDD I ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA
Sbjct: 243 ----------ASPQANLSDDLIRALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 302

Query: 310 CLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKP 369
           CLKGLNGVP+VVEC+FVQST+TELPFFASKV+LGKNGVE+VLDLG LSDFEKEGLE LK 
Sbjct: 303 CLKGLNGVPNVVECSFVQSTITELPFFASKVRLGKNGVEEVLDLGPLSDFEKEGLEALKA 340

Query: 370 ELKASIEKGIQFAN 382
           ELK+SIEKGI+FAN
Sbjct: 363 ELKSSIEKGIKFAN 340

[Close]

BLAST of Carg11350 vs. ExPASy TrEMBL
Match: A0A6J1JAL7 (Malate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111482765 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.0e-181
Identity = 346/383 (90.34%), Postives = 347/383 (90.60%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKASLLRSVRSAVSRSSSSNHLLRRSFGTE+VPERKVTVLGAAGGIGQPLSLLMKLNPLV
Sbjct: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTESVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKANAPVS                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANAPVS-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

[Close]

BLAST of Carg11350 vs. ExPASy TrEMBL
Match: A0A6J1EAA5 (Malate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111430790 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.1e-180
Identity = 345/383 (90.08%), Postives = 347/383 (90.60%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKASLLRSVRSAVSRSS+SNHLLRRSFGTE+VPERKVTVLGAAGGIGQPLSLLMKLNPLV
Sbjct: 1   MKASLLRSVRSAVSRSSASNHLLRRSFGTESVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKANAPVS                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANAPVS-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

[Close]

BLAST of Carg11350 vs. ExPASy TrEMBL
Match: A0A161GC69 (Malate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103492923 PE=2 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 327/383 (85.38%), Postives = 337/383 (87.99%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKAS+LRSVRSAVSRSSSSN LL R+F TE+VPERKV VLGAAGGIGQPL+LLMKLNPLV
Sbjct: 1   MKASILRSVRSAVSRSSSSNRLLTRTFATESVPERKVAVLGAAGGIGQPLALLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           S+LALYDIAGTPGVAADVGHVNTRSEVTGY GEEQL KALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SKLALYDIAGTPGVAADVGHVNTRSEVTGYVGEEQLGKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKA TYDE+KLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKANTYDEKKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKAN PV+                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANVPVA-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANL+DDTI+ALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLTDDTIVALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVEC+FVQSTVTELPFFASKVKLGKNGVE VLDLG LSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECSFVQSTVTELPFFASKVKLGKNGVESVLDLGPLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

[Close]

BLAST of Carg11350 vs. ExPASy TrEMBL
Match: A0A5A7UUX9 (Malate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold416G00740 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 327/383 (85.38%), Postives = 337/383 (87.99%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKAS+LRSVRSAVSRSSSSN LL R+F TE+VPERKV VLGAAGGIGQPL+LLMKLNPLV
Sbjct: 1   MKASILRSVRSAVSRSSSSNRLLTRTFATESVPERKVAVLGAAGGIGQPLALLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           S+LALYDIAGTPGVAADVGHVNTRSEVTGY GEEQL KALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SKLALYDIAGTPGVAADVGHVNTRSEVTGYVGEEQLGKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKA TYDE+KLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKANTYDEKKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKAN PV+                       EVNVPVIGGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANVPVA-----------------------EVNVPVIGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANL+DDTI+ALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLTDDTIVALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVEC+FVQSTVTELPFFASKVKLGKNGVE VLDLG LSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECSFVQSTVTELPFFASKVKLGKNGVESVLDLGPLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKLKPELKASIEKGIQFANAN
Sbjct: 361 GLEKLKPELKASIEKGIQFANAN 347

[Close]

BLAST of Carg11350 vs. ExPASy TrEMBL
Match: A0A0A0L0E4 (Malate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G083700 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.6e-171
Identity = 325/383 (84.86%), Postives = 336/383 (87.73%), Query Frame = 0


Query: 1   MKASLLRSVRSAVSRSSSSNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLV 60
           MKAS+LRSVRSAVSRSSSSNHLL R+F TE+VPERKV VLGAAGGIGQPL+LLMKLNPLV
Sbjct: 1   MKASILRSVRSAVSRSSSSNHLLTRTFATESVPERKVAVLGAAGGIGQPLALLMKLNPLV 60

Query: 61  SRLALYDIAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTR 120
           S+LALYDI  TPGVAADVGHVNTRSEVTGY GEEQL KALEGSDVVIIPAGVPRKPGMTR
Sbjct: 61  SKLALYDIGATPGVAADVGHVNTRSEVTGYMGEEQLGKALEGSDVVIIPAGVPRKPGMTR 120

Query: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVT 180
           DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDE+KLFGVT
Sbjct: 121 DDLFNINAGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDEKKLFGVT 180

Query: 181 TLDVVRAKTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILP 240
           TLDVVRAKTFYAGKAN PV+                       EVNVPV+GGHAGITILP
Sbjct: 181 TLDVVRAKTFYAGKANVPVA-----------------------EVNVPVVGGHAGITILP 240

Query: 241 LFSQCSCIGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300
           LFSQ             ATPKANL+DDTI+ALTKRTQDGGTEVVEAKAGKGSATLSMAYA
Sbjct: 241 LFSQ-------------ATPKANLTDDTIVALTKRTQDGGTEVVEAKAGKGSATLSMAYA 300

Query: 301 GALFADACLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKE 360
           GALFADACLKGLNGVPDVVEC+FVQSTVTELPFFASKVKLGKNGVE VLDLG LSDFEKE
Sbjct: 301 GALFADACLKGLNGVPDVVECSFVQSTVTELPFFASKVKLGKNGVESVLDLGPLSDFEKE 347

Query: 361 GLEKLKPELKASIEKGIQFANAN 384
           GLEKL PELKASIEKGIQFANAN
Sbjct: 361 GLEKLMPELKASIEKGIQFANAN 347

[Close]

BLAST of Carg11350 vs. TAIR 10
Match: AT1G53240.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 545.8 bits (1405), Expect = 2.7e-155
Identity = 284/374 (75.94%), Postives = 317/374 (84.76%), Query Frame = 0


Query: 10  RSAVSRSSSS--NHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYD 69
           RS + RSS+S    ++RRSF + +VPERKV +LGAAGGIGQPL+LLMKLNPLVS L+LYD
Sbjct: 3   RSMLVRSSASAKQAVIRRSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYD 62

Query: 70  IAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNIN 129
           IA TPGVAADVGH+NTRSEV GY G++ LAKALEG+D+VIIPAGVPRKPGMTRDDLFNIN
Sbjct: 63  IANTPGVAADVGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNIN 122

Query: 130 AGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRA 189
           AGIVK+LCTAIAKYCP+AL+NMISNPVNSTVPIAAE+FKKAG YDE+KLFGVTTLDVVRA
Sbjct: 123 AGIVKNLCTAIAKYCPHALINMISNPVNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRA 182

Query: 190 KTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSC 249
           +TFYAGKAN PV+                       EVNVPVIGGHAG+TILPLFSQ   
Sbjct: 183 RTFYAGKANVPVA-----------------------EVNVPVIGGHAGVTILPLFSQ--- 242

Query: 250 IGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 309
                     ATP+ANLS D + ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA
Sbjct: 243 ----------ATPQANLSSDILTALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 302

Query: 310 CLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKP 369
           CLKGLNGVPDV+EC++VQST+TELPFFASKV+LGKNGVE+VLDLG LSDFEKEGLE LKP
Sbjct: 303 CLKGLNGVPDVIECSYVQSTITELPFFASKVRLGKNGVEEVLDLGPLSDFEKEGLEALKP 340

Query: 370 ELKASIEKGIQFAN 382
           ELK+SIEKG++FAN
Sbjct: 363 ELKSSIEKGVKFAN 340

[Close]

BLAST of Carg11350 vs. TAIR 10
Match: AT3G15020.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 533.5 bits (1373), Expect = 1.4e-151
Identity = 282/374 (75.40%), Postives = 312/374 (83.42%), Query Frame = 0


Query: 10  RSAVSRSSS--SNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYD 69
           RS + RS+S     LLRR F +E+VP+RKV +LGAAGGIGQPLSLLMKLNPLVS L+LYD
Sbjct: 3   RSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSLYD 62

Query: 70  IAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNIN 129
           IA TPGVAADVGH+NTRS+V+GY G++ L KALEG+D+VIIPAGVPRKPGMTRDDLFNIN
Sbjct: 63  IANTPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLFNIN 122

Query: 130 AGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRA 189
           AGIVK+L  AIAKYCP ALVNMISNPVNSTVPIAAE+FKKAGTYDE+KLFGVTTLDVVRA
Sbjct: 123 AGIVKNLSIAIAKYCPQALVNMISNPVNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVVRA 182

Query: 190 KTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSC 249
           +TFYAGK++                         V EVNVPV+GGHAGITILPLFSQ   
Sbjct: 183 RTFYAGKSDV-----------------------NVAEVNVPVVGGHAGITILPLFSQ--- 242

Query: 250 IGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 309
                     A+P+ANLSDD I ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA
Sbjct: 243 ----------ASPQANLSDDLIRALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 302

Query: 310 CLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKP 369
           CLKGLNGVP+VVEC+FVQST+TELPFFASKV+LGKNGVE+VLDLG LSDFEKEGLE LK 
Sbjct: 303 CLKGLNGVPNVVECSFVQSTITELPFFASKVRLGKNGVEEVLDLGPLSDFEKEGLEALKA 340

Query: 370 ELKASIEKGIQFAN 382
           ELK+SIEKGI+FAN
Sbjct: 363 ELKSSIEKGIKFAN 340

[Close]

BLAST of Carg11350 vs. TAIR 10
Match: AT3G15020.2 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 469.2 bits (1206), Expect = 3.3e-132
Identity = 248/335 (74.03%), Postives = 275/335 (82.09%), Query Frame = 0


Query: 10  RSAVSRSSS--SNHLLRRSFGTEAVPERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYD 69
           RS + RS+S     LLRR F +E+VP+RKV +LGAAGGIGQPLSLLMKLNPLVS L+LYD
Sbjct: 3   RSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSLYD 62

Query: 70  IAGTPGVAADVGHVNTRSEVTGYAGEEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNIN 129
           IA TPGVAADVGH+NTRS+V+GY G++ L KALEG+D+VIIPAGVPRKPGMTRDDLFNIN
Sbjct: 63  IANTPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLFNIN 122

Query: 130 AGIVKSLCTAIAKYCPNALVNMISNPVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRA 189
           AGIVK+L  AIAKYCP ALVNMISNPVNSTVPIAAE+FKKAGTYDE+KLFGVTTLDVVRA
Sbjct: 123 AGIVKNLSIAIAKYCPQALVNMISNPVNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVVRA 182

Query: 190 KTFYAGKANAPVSGISRCYNSEQGQTLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSC 249
           +TFYAGK++                         V EVNVPV+GGHAGITILPLFSQ   
Sbjct: 183 RTFYAGKSDV-----------------------NVAEVNVPVVGGHAGITILPLFSQ--- 242

Query: 250 IGCFGLEGLVATPKANLSDDTILALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 309
                     A+P+ANLSDD I ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA
Sbjct: 243 ----------ASPQANLSDDLIRALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADA 301

Query: 310 CLKGLNGVPDVVECTFVQSTVTELPFFASKVKLGK 343
           CLKGLNGVP+VVEC+FVQST+TELPFFASKV+ GK
Sbjct: 303 CLKGLNGVPNVVECSFVQSTITELPFFASKVRTGK 301

[Close]

BLAST of Carg11350 vs. TAIR 10
Match: AT5G09660.1 (peroxisomal NAD-malate dehydrogenase 2 )

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 209/347 (60.23%), Postives = 246/347 (70.89%), Query Frame = 0


Query: 33  PERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYDIAGTPGVAADVGHVNTRSEVTGYAG 92
           P  KV +LGAAGGIGQ LSLLMK+NPLVS L LYD+   PGV ADV H++T + V G+ G
Sbjct: 41  PGFKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLG 100

Query: 93  EEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNINAGIVKSLCTAIAKYCPNALVNMISN 152
            +QL  AL G D+VIIPAG+PRKPGMTRDDLF INAGIVK+LC  +AK CPNA+VN+ISN
Sbjct: 101 AKQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISN 160

Query: 153 PVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRAKTFYAGKANAPVSGISRCYNSEQGQ 212
           PVNSTVPIAAEVFKKAGTYD +KL GVTTLDV RA TF      A V G+          
Sbjct: 161 PVNSTVPIAAEVFKKAGTYDPKKLLGVTTLDVARANTFV-----AEVLGLD--------- 220

Query: 213 TLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSCIGCFGLEGLVATPKANLSDDTILAL 272
                      EV+VPV+GGHAG+TILPL SQ               P ++ +   I  L
Sbjct: 221 ---------PREVDVPVVGGHAGVTILPLLSQ-------------VKPPSSFTPQEIEYL 280

Query: 273 TKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLKGLNGVPDVVECTFVQSTVTELP 332
           T R Q+GGTEVVEAKAG GSATLSMAYA A FADACL+GL G  +VVEC+FV S VTEL 
Sbjct: 281 TNRIQNGGTEVVEAKAGAGSATLSMAYAAAKFADACLRGLRGDANVVECSFVASQVTELA 340

Query: 333 FFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKPELKASIEKGIQF 380
           FFA+KV+LG+ G E+V  LG L+++E+ GLEK K EL  SI+KG++F
Sbjct: 341 FFATKVRLGRTGAEEVYQLGPLNEYERIGLEKAKDELAGSIQKGVEF 351

[Close]

BLAST of Carg11350 vs. TAIR 10
Match: AT5G09660.2 (peroxisomal NAD-malate dehydrogenase 2 )

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 209/347 (60.23%), Postives = 246/347 (70.89%), Query Frame = 0


Query: 33  PERKVTVLGAAGGIGQPLSLLMKLNPLVSRLALYDIAGTPGVAADVGHVNTRSEVTGYAG 92
           P  KV +LGAAGGIGQ LSLLMK+NPLVS L LYD+   PGV ADV H++T + V G+ G
Sbjct: 20  PGFKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLG 79

Query: 93  EEQLAKALEGSDVVIIPAGVPRKPGMTRDDLFNINAGIVKSLCTAIAKYCPNALVNMISN 152
            +QL  AL G D+VIIPAG+PRKPGMTRDDLF INAGIVK+LC  +AK CPNA+VN+ISN
Sbjct: 80  AKQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISN 139

Query: 153 PVNSTVPIAAEVFKKAGTYDERKLFGVTTLDVVRAKTFYAGKANAPVSGISRCYNSEQGQ 212
           PVNSTVPIAAEVFKKAGTYD +KL GVTTLDV RA TF      A V G+          
Sbjct: 140 PVNSTVPIAAEVFKKAGTYDPKKLLGVTTLDVARANTFV-----AEVLGLD--------- 199

Query: 213 TLLPDCCNTVTEVNVPVIGGHAGITILPLFSQCSCIGCFGLEGLVATPKANLSDDTILAL 272
                      EV+VPV+GGHAG+TILPL SQ               P ++ +   I  L
Sbjct: 200 ---------PREVDVPVVGGHAGVTILPLLSQ-------------VKPPSSFTPQEIEYL 259

Query: 273 TKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLKGLNGVPDVVECTFVQSTVTELP 332
           T R Q+GGTEVVEAKAG GSATLSMAYA A FADACL+GL G  +VVEC+FV S VTEL 
Sbjct: 260 TNRIQNGGTEVVEAKAGAGSATLSMAYAAAKFADACLRGLRGDANVVECSFVASQVTELA 319

Query: 333 FFASKVKLGKNGVEQVLDLGTLSDFEKEGLEKLKPELKASIEKGIQF 380
           FFA+KV+LG+ G E+V  LG L+++E+ GLEK K EL  SI+KG++F
Sbjct: 320 FFATKVRLGRTGAEEVYQLGPLNEYERIGLEKAKDELAGSIQKGVEF 330

The following BLAST results are available for this feature:

BLAST of Carg11350 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015040.1	1.5e-211	100.00	Malate dehydrogenase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022984474.1	1.0e-180	90.34	malate dehydrogenase, mitochondrial [Cucurbita maxima] >XP_023552175.1 malate de...	[more]
XP_022922965.1	2.3e-180	90.08	malate dehydrogenase, mitochondrial [Cucurbita moschata]	[more]
XP_038878007.1	2.1e-173	86.16	malate dehydrogenase, mitochondrial [Benincasa hispida]	[more]
P17783.1	3.0e-172	85.64	RecName: Full=Malate dehydrogenase, mitochondrial; AltName: Allergen=Citr l MDH;...	[more]

BLAST of Carg11350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17783	4.0e-175	85.64	Malate dehydrogenase, mitochondrial OS=Citrullus lanatus OX=3654 GN=MMDH PE=1 SV...	[more]
P46487	7.5e-158	77.23	Malate dehydrogenase, mitochondrial OS=Eucalyptus gunnii OX=3933 GN=MDH PE=2 SV=...	[more]
Q9ZP06	3.9e-154	75.94	Malate dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g532...	[more]
P83373	1.8e-151	75.34	Malate dehydrogenase, mitochondrial OS=Fragaria ananassa OX=3747 GN=MMDHI PE=1 S...	[more]
Q9LKA3	2.0e-150	75.40	Malate dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g150...	[more]

BLAST of Carg11350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JAL7	5.0e-181	90.34	Malate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111482765 PE=3 SV=1	[more]
A0A6J1EAA5	1.1e-180	90.08	Malate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111430790 PE=3 SV=1	[more]
A0A161GC69	1.2e-171	85.38	Malate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103492923 PE=2 SV=1	[more]
A0A5A7UUX9	1.2e-171	85.38	Malate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold416...	[more]
A0A0A0L0E4	3.6e-171	84.86	Malate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_4G083700 PE=3 SV=1	[more]

BLAST of Carg11350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G53240.1	2.7e-155	75.94	Lactate/malate dehydrogenase family protein	[more]
AT3G15020.1	1.4e-151	75.40	Lactate/malate dehydrogenase family protein	[more]
AT3G15020.2	3.3e-132	74.03	Lactate/malate dehydrogenase family protein	[more]
AT5G09660.1	1.2e-107	60.23	peroxisomal NAD-malate dehydrogenase 2	[more]
AT5G09660.2	1.2e-107	60.23	peroxisomal NAD-malate dehydrogenase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 36..178 e-value: 4.9E-48 score: 162.8
None	No IPR available	GENE3D	3.40.50.720		coord: 34..179 e-value: 7.7E-59 score: 199.9
None	No IPR available	PIRSR	PIRSR000102-1	PIRSR000102-1	coord: 35..380 e-value: 1.2E-75 score: 252.4
None	No IPR available	PANTHER	PTHR11540	MALATE AND LACTATE DEHYDROGENASE	coord: 221..383 coord: 1..202
None	No IPR available	PANTHER	PTHR11540:SF57	MALATE DEHYDROGENASE	coord: 221..383 coord: 1..202
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..379 e-value: 1.9E-41 score: 141.9
IPR001557	L-lactate/malate dehydrogenase	PIRSF	PIRSF000102	Lac_mal_DH	coord: 32..381 e-value: 1.2E-58 score: 196.6
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	3.90.110.10		coord: 180..383 e-value: 1.5E-59 score: 203.2
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	SUPERFAMILY	56327	LDH C-terminal domain-like	coord: 222..381
IPR010097	Malate dehydrogenase, type 1	TIGRFAM	TIGR01772	TIGR01772	coord: 36..381 e-value: 2.4E-119 score: 396.2
IPR010097	Malate dehydrogenase, type 1	CDD	cd01337	MDH_glyoxysomal_mitochondrial	coord: 36..380 e-value: 0.0 score: 541.695
IPR001252	Malate dehydrogenase, active site	PROSITE	PS00068	MDH	coord: 179..191
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 36..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11350-RA	Carg11350-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006099	tricarboxylic acid cycle
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0030060	L-malate dehydrogenase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016615	malate dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor