Carg11334 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11334
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description3-hydroxybutyryl-CoA dehydrogenase
LocationCarg_Chr16: 2269222 .. 2272751 (-)
RNA-Seq ExpressionCarg11334
SyntenyCarg11334
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATCTTTTCGTCTTTATCCATTTTTTTGATAAAAGCCTGACAGAGATGAATTCCACAGGAGGATAATCGAATGATTCTTTCTCCGATTACAGTAATCGCCGCCAGATTTGTGGTTTCTCGATGACGGAAATCAAGGTTATTGGTGTCGTAGGCGGCGGTCAAATGGGCTCCGGAATTGCCCAACTCGCCGCCGCATACGGCCACGATGTTTGGCTAATTGATTCAGACCCTGCCGCTCTTACTAAAGCAACCAACTCCATTTCTTCCTCAATCCAACGCCTGGTCTCCAGAAACATTCTCTCTCGAGTAAGAATTCTGTATTTCCAGATCCGTATACATGGGAAGAATATATATATATATATGATGTTTGGACTATAATTTACTCTGACATAGTTCTTCTGAATTGGCTGTTTTACCTTTTGATTTACCTTTTATTTGGAGTTTTGTGTTGTCTATTTCATCATAAAAGCTCTTAACAGACCAATTGCAGGGAATCACAAGGTGTAGGACATGTTACAGACTGACTTTGAAATGTTTGAAATAGTTTTTGTCAAGTAAAAAATCAATTCGAAACCTCGGTTTAATGATTCAAAACTAGTTTAATGTTTGATTTTAGACTTCTAAACACGATTTTGATACGATTAAAACTGATTTTTGAATCATAAGAATATTATTTCGAGTGATTTTGAAAGCTCTCAAACATGCACTTGATTTAGGTGAAAAACGCAGGATTTAGTGCTATTTGGTTGTTTACTTATGATCAATCTGTAGCATTAGGAGGTTGAGACTCTCTTGGCCCCATCAGTACCCTTTCTAATCAGGATAACACCGATCCACTGCTTTAGGAAAAAGAAAAACAAGAGAATCCTTGCCAGATCCCATGTCTCTGTAATTTCAGCTCATGATATTCTTGGGTCATTCCATACTGAAGGACTAGGAAAATTTGGTCCATAATCTCGATCCTGTCACGACGACTGTGCGTTTGGATGGCAGTGATAGTACCAAACATGAAAAATAGTGTGATCTTGAGCCAAAGCTGTAGATCAATAATTGAACTTAAAAATAGACTTATCCAAATGACACAGGATCATAGAGGAATTGGATTTTGATTGAGGGAGTCAATGAAACAAAAGTGGACACTGATGAGCTTCTCTTTAGCTTATGAAGAAGCCACGAGATGATGATTTTCTTATAGCAGCTTCAACCTTTTGCTTTAAACAATAAGAACTTACAGGCTTTTAGAAGCCATTGGATGCCTTAAATAGAGGTTTTCTTTTGTCTGTGTGTGTGTGTGTTTGGCTAGGGGTTAAGCAAGTATTTGGGTAGTGTGGGATATAGAACTGTGCCTCTTGAATGTTTATTTAATCTTTGTATTTCTATCTCTTTTAGTACTTAGACTAATAAAATATCCTTTTTGATGTCCTTTCAAATTCTGGCACGTACAATATTTTGCTCTAGCCTATGGCCTTTGATGCAAAATAAATGTTTTATCCCCATTGACCATTTGATCCGAAGTTAAACTATGAGATTTTGAATCGGTTGGAGAGGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCTCTTCCTACCGTGAGGCTAACGGCAATACGTAACGGACCAAAGCGGACAATATTTGTTAGCTGTGGGCTTGAGCTGTTGCAAAAGGTATTAGAGCCAGGCACCGGGGGGTGTGCCAACGAGGAATCTGTGCTCCCAAGGGAGGTGGATTGTGAGATCCCACATCGGTTGGAGAGAGGAACAAAGCATTTTCTTATAAGGATGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAACCGTGAGGCTGACGATGATACGTAATGGGCTAAATCGGACAATATCTACTAGCGGTGGGCTTGGGCTGTTATATAAACCGAGTTATTTACCGCAGGTTTGACATTGATTATGGAAAGGTTGACATACAAATCTATAACAGAGAAGTTCTACCGGCCAAATGAGCTTCTCTCTGCCGTTGAAATTGTAGACATTTAATCAGCTTATGAAATCCACTTTACAGGAAGTTGGTGTCGATGCTCTTCAACGTCTAAAATGTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATAGAAGCTATCGTGGAATCAGAAGATGTTAAAAGAAAGCTATTTCTTGAATTGGATAAGATTGCAAAACCATCTGCAATTCTAGCTTCTAATACAAGTTCCATCCCCATCACTCGCTTAGCATCGTCGACCACACGTCCCGAGAAGGTTTTGTCCTCTCTTCCCCCCCTTGCATGAATGCTGAACTTCTGTTTTTCTTTTAAAGTGCCATTTGTACGTAGTTTTAATAGCTAGCCATGTGATTCGGCGATAAAACGCGTTGTTATGTGATTTTAGCATGCATTATATGAAACAGAAACGAAGAACTAATTTACTAAACGTTTAACGCCATTGCAGGTTATTGGCATGCATTTCATGAATCCACCACCTATAATGAAGTTGGTTGAGATTGTTCGAGGTGCGGATACATCCGATGAAACGTTTCATGCAATAAAAACCTTGGCTGAGAGGTGATTCACTTCGTCGATTGCTCTTAAATTCGCCTCCGCCCCTCTGTCCCTCCTCTACCATCTGTTGTCTTTCTAGTAACATGGGACTACTGCACTTAGTACATGAGTTGGCTAAGCGTAGTCATAACTAACAACTCTCACTATATCGTTGCGTTTGGGCCTCGAACGAACATGTACCGAGAACAAATAATAGTTTTAACTTTCTTTCCTTTCAGGCTTGGCAAAACATTGATATGCTCTCAGGACTACTCCGGCTTCATTGTGAACAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTCGCTCTCTACACGGGAGTGGCAACGAAGGAAGACATCGACACAGGAATGAAGCTAGGAACAAACCATCCCATGGGTCCCTTGGAGCTTGCAGACTTAATTGGTTTGGATGTTTGTTTATCAATCATGAAGGTACTTCACTCTGGACTTGGTGATGATAAATATGCTCCCTGCCCTCTTCTCGTGCAGTATGTCGACGCTGGTCGCCTCGGAAGAAAGCGAGGATTAGGCGTATACGGTTACCGTAAATTACCAAAACCAGAAAAATCTAATTCTCGACTTTGATTCATTCGAGTCTATAAGATAAATATGTGACATGGATGATCCTGCTCTCAATACTCTCTTATACTGTCTCAATAAACTGCTACTTCATTTACGTTTGAAACTTTGAAGTTGACATTTATTTGGTACCAAACTCACAATACCAAGCCTAACACATTAAATTTTTAAATATCTTGGACATAGACGTGACGTAGATAAATGATTTGGATGTTTGTTTATCACGGTGATACATAATTTTTGTTCTGACGGTGATACATAACTCTCGCAGTGATACATAATTTTTGTTTATCACGGTGATACCAAATCCAAGTATGGTACGTTTATGACAGTGATACATAATTTTTTAAATATATAGGACACCGTAGATAGATGATTTTTGTTATTT

mRNA sequence

ATATCTTTTCGTCTTTATCCATTTTTTTGATAAAAGCCTGACAGAGATGAATTCCACAGGAGGATAATCGAATGATTCTTTCTCCGATTACAGTAATCGCCGCCAGATTTGTGGTTTCTCGATGACGGAAATCAAGGTTATTGGTGTCGTAGGCGGCGGTCAAATGGGCTCCGGAATTGCCCAACTCGCCGCCGCATACGGCCACGATGTTTGGCTAATTGATTCAGACCCTGCCGCTCTTACTAAAGCAACCAACTCCATTTCTTCCTCAATCCAACGCCTGGTCTCCAGAAACATTCTCTCTCGAGAAGTTGGTGTCGATGCTCTTCAACGTCTAAAATGTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATAGAAGCTATCGTGGAATCAGAAGATGTTAAAAGAAAGCTATTTCTTGAATTGGATAAGATTGCAAAACCATCTGCAATTCTAGCTTCTAATACAAGTTCCATCCCCATCACTCGCTTAGCATCGTCGACCACACGTCCCGAGAAGGTTATTGGCATGCATTTCATGAATCCACCACCTATAATGAAGTTGGTTGAGATTGTTCGAGGTGCGGATACATCCGATGAAACGTTTCATGCAATAAAAACCTTGGCTGAGAGGCTTGGCAAAACATTGATATGCTCTCAGGACTACTCCGGCTTCATTGTGAACAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTCGCTCTCTACACGGGAGTGGCAACGAAGGAAGACATCGACACAGGAATGAAGCTAGGAACAAACCATCCCATGGGTCCCTTGGAGCTTGCAGACTTAATTGGTTTGGATGTTTGTTTATCAATCATGAAGGTACTTCACTCTGGACTTGGTGATGATAAATATGCTCCCTGCCCTCTTCTCGTGCAGTATGTCGACGCTGGTCGCCTCGGAAGAAAGCGAGGATTAGGCGTATACGGTTACCGTAAATTACCAAAACCAGAAAAATCTAATTCTCGACTTTGATTCATTCGAGTCTATAAGATAAATATGTGACATGGATGATCCTGCTCTCAATACTCTCTTATACTGTCTCAATAAACTGCTACTTCATTTACGTTTGAAACTTTGAAGTTGACATTTATTTGGTACCAAACTCACAATACCAAGCCTAACACATTAAATTTTTAAATATCTTGGACATAGACGTGACGTAGATAAATGATTTGGATGTTTGTTTATCACGGTGATACATAATTTTTGTTCTGACGGTGATACATAACTCTCGCAGTGATACATAATTTTTGTTTATCACGGTGATACCAAATCCAAGTATGGTACGTTTATGACAGTGATACATAATTTTTTAAATATATAGGACACCGTAGATAGATGATTTTTGTTATTT

Coding sequence (CDS)

ATGACGGAAATCAAGGTTATTGGTGTCGTAGGCGGCGGTCAAATGGGCTCCGGAATTGCCCAACTCGCCGCCGCATACGGCCACGATGTTTGGCTAATTGATTCAGACCCTGCCGCTCTTACTAAAGCAACCAACTCCATTTCTTCCTCAATCCAACGCCTGGTCTCCAGAAACATTCTCTCTCGAGAAGTTGGTGTCGATGCTCTTCAACGTCTAAAATGTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATAGAAGCTATCGTGGAATCAGAAGATGTTAAAAGAAAGCTATTTCTTGAATTGGATAAGATTGCAAAACCATCTGCAATTCTAGCTTCTAATACAAGTTCCATCCCCATCACTCGCTTAGCATCGTCGACCACACGTCCCGAGAAGGTTATTGGCATGCATTTCATGAATCCACCACCTATAATGAAGTTGGTTGAGATTGTTCGAGGTGCGGATACATCCGATGAAACGTTTCATGCAATAAAAACCTTGGCTGAGAGGCTTGGCAAAACATTGATATGCTCTCAGGACTACTCCGGCTTCATTGTGAACAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTCGCTCTCTACACGGGAGTGGCAACGAAGGAAGACATCGACACAGGAATGAAGCTAGGAACAAACCATCCCATGGGTCCCTTGGAGCTTGCAGACTTAATTGGTTTGGATGTTTGTTTATCAATCATGAAGGTACTTCACTCTGGACTTGGTGATGATAAATATGCTCCCTGCCCTCTTCTCGTGCAGTATGTCGACGCTGGTCGCCTCGGAAGAAAGCGAGGATTAGGCGTATACGGTTACCGTAAATTACCAAAACCAGAAAAATCTAATTCTCGACTTTGA

Protein sequence

MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDVCLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL
Homology
BLAST of Carg11334 vs. NCBI nr
Match: KAG7015024.1 (hbdA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 577.8 bits (1488), Expect = 5.4e-161
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL
Sbjct: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI
Sbjct: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL
Sbjct: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 296

BLAST of Carg11334 vs. NCBI nr
Match: XP_022922687.1 (uncharacterized protein LOC111430610 [Cucurbita moschata] >XP_022922688.1 uncharacterized protein LOC111430610 [Cucurbita moschata])

HSP 1 Score: 573.9 bits (1478), Expect = 7.8e-160
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL
Sbjct: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SREVGVD LQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVDVLQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI
Sbjct: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKS SRL
Sbjct: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSKSRL 296

BLAST of Carg11334 vs. NCBI nr
Match: XP_023553026.1 (uncharacterized protein LOC111810545 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.0 bits (1460), Expect = 9.5e-158
Identity = 289/296 (97.64%), Postives = 293/296 (98.99%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRN+L
Sbjct: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNLL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SREVGV ALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVSALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLASSTTRP+KVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI
Sbjct: 121 SIPITRLASSTTRPDKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           C+SIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLG+Y YRKLPKPEKS SRL
Sbjct: 241 CVSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGIYDYRKLPKPEKSKSRL 296

BLAST of Carg11334 vs. NCBI nr
Match: XP_022984420.1 (uncharacterized protein LOC111482724 [Cucurbita maxima])

HSP 1 Score: 562.0 bits (1447), Expect = 3.1e-156
Identity = 286/296 (96.62%), Postives = 291/296 (98.31%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIKVIGV+GGGQMGSGIAQLAAAYGHD+WLIDSDPAALTKATNSISSSIQRLVSRN+L
Sbjct: 1   MTEIKVIGVIGGGQMGSGIAQLAAAYGHDIWLIDSDPAALTKATNSISSSIQRLVSRNLL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SREVGVDALQRLKCSTDLKELHSADVIIEA+VESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAVVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRG DTSDETF AIKTLAERLGKTLI
Sbjct: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGVDTSDETFDAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNR+LMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYSGFIVNRLLMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVL SGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVY YRKLPKPEKS SRL
Sbjct: 241 CLSIMKVLQSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYDYRKLPKPEKSKSRL 296

BLAST of Carg11334 vs. NCBI nr
Match: KAG6577002.1 (Protein DETOXIFICATION 19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554.3 bits (1427), Expect = 6.4e-154
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL
Sbjct: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI
Sbjct: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYR 285
           CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYR
Sbjct: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYR 284

BLAST of Carg11334 vs. ExPASy Swiss-Prot
Match: Q45223 (3-hydroxybutyryl-CoA dehydrogenase OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=hbdA PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.2e-82
Identity = 156/281 (55.52%), Postives = 196/281 (69.75%), Query Frame = 0

Query: 4   IKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSRE 63
           IK +GV+G GQMG+GIA +AA  G DV L D     L     +I+ ++ R VS+ +++ E
Sbjct: 5   IKKVGVIGAGQMGNGIAHVAALAGFDVVLNDVSADRLKSGMATINGNLARQVSKKVVTEE 64

Query: 64  VGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIP 123
               AL R+  +  L +L   D++IE  VE E+VKRK+F EL  + KP AI+AS+TSSI 
Sbjct: 65  AKTKALSRIVAAEKLDDLADCDLVIETAVEKEEVKRKIFHELCAVLKPEAIVASDTSSIS 124

Query: 124 ITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICSQ 183
           ITRLA++T RPE+ IG+HFMNP P+M+LVE++RG  T D TF A K    +LGK +  S+
Sbjct: 125 ITRLAAATDRPERFIGIHFMNPVPLMELVELIRGIATDDATFEASKEFVAKLGKQVAVSE 184

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDVCLS 243
           D+  FIVNRIL+PMINEA + LY GV   E ID  MKLG +HPMGPLELAD IGLD CLS
Sbjct: 185 DFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDAAMKLGAHHPMGPLELADFIGLDTCLS 244

Query: 244 IMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYR 285
           IM+VLH GL D KY PCPLLV+YV+AG LGRK   G Y YR
Sbjct: 245 IMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKTQRGFYDYR 285

BLAST of Carg11334 vs. ExPASy Swiss-Prot
Match: P45856 (Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Bacillus subtilis (strain 168) OX=224308 GN=mmgB PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.9e-77
Identity = 150/283 (53.00%), Postives = 188/283 (66.43%), Query Frame = 0

Query: 3   EIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSR 62
           EIK I V G GQMGSGIAQ AA  G  V + D +P A       +   + R   +   + 
Sbjct: 2   EIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRTE 61

Query: 63  EVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSI 122
                 + R+  S  L+E   AD++IEAI E+   K ++F  LD+I  P  ILASNTSS+
Sbjct: 62  TEVKSVINRISISQTLEEAEHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSSL 121

Query: 123 PITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICS 182
           PIT +A+ T RP++VIGMHFMNP P+MKLVE++RG  TS+ET   +  LAE++GKT +  
Sbjct: 122 PITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATSEETALDVMALAEKMGKTAVEV 181

Query: 183 QDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDVCL 242
            D+ GF+ NR+L+PMINEA + +Y GVA  E ID  MKLG NHPMGPL LAD IGLD CL
Sbjct: 182 NDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTCL 241

Query: 243 SIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRK 286
           SIM+VLHSGLGD KY PCPLL +YV AG LG+K G G Y Y +
Sbjct: 242 SIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEE 284

BLAST of Carg11334 vs. ExPASy Swiss-Prot
Match: Q9RVG1 (Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=hbd PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.8e-74
Identity = 148/276 (53.62%), Postives = 187/276 (67.75%), Query Frame = 0

Query: 8   GVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSREVGVD 67
           GV+G GQMG GIAQ+AA  G DV + D     L +    I  S+ +L  +  L+ +    
Sbjct: 4   GVIGAGQMGGGIAQVAAQSGFDVVVHDQKQEFLDRGKGVIEKSLGKLHEKGKLT-DAPET 63

Query: 68  ALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIPITRL 127
            L R+  +TDL++    D+++EAIVE++ +K  LF +L +I KP  ILASNTSSIPIT L
Sbjct: 64  VLGRIHFTTDLQDFADCDLVVEAIVENQQIKNDLFKQLGQIVKPEGILASNTSSIPITAL 123

Query: 128 ASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICSQDYSG 187
           A+++ RP + IGMHFMNP P+M+LVE++RG  TSDET   +   AE+LGKT +   D+ G
Sbjct: 124 ATASGRPAQFIGMHFMNPVPLMQLVEVIRGYQTSDETARIVTETAEKLGKTPLSCNDFPG 183

Query: 188 FIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDVCLSIMKV 247
           F+ NRILMPM+NEA   +  GVA  E ID  MKLG NHPMGPL LAD IGLD CL+IM+V
Sbjct: 184 FVSNRILMPMLNEAIQCVMEGVAEPEAIDGIMKLGMNHPMGPLTLADFIGLDTCLAIMEV 243

Query: 248 LHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGY 284
           LH GLGDDKY P PLL + V AG LGRK G G Y Y
Sbjct: 244 LHQGLGDDKYRPSPLLRKMVQAGLLGRKSGEGFYKY 278

BLAST of Carg11334 vs. ExPASy Swiss-Prot
Match: P52041 (3-hydroxybutyryl-CoA dehydrogenase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=hbd PE=1 SV=2)

HSP 1 Score: 276.6 bits (706), Expect = 3.4e-73
Identity = 145/282 (51.42%), Postives = 188/282 (66.67%), Query Frame = 0

Query: 4   IKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSRE 63
           +K + V+G G MGSGIAQ  AA G +V L D     + +  + I+ ++ +LV +  +   
Sbjct: 1   MKKVCVIGAGTMGSGIAQAFAAKGFEVVLRDIKDEFVDRGLDFINKNLSKLVKKGKIEEA 60

Query: 64  VGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIP 123
             V+ L R+  + DL      D++IEA VE  D+K+++F +LD I KP  ILASNTSS+ 
Sbjct: 61  TKVEILTRISGTVDLNMAADCDLVIEAAVERMDIKKQIFADLDNICKPETILASNTSSLS 120

Query: 124 ITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICSQ 183
           IT +AS+T RP+KVIGMHF NP P+MKLVE++RG  TS ETF A+K  +  +GK  +   
Sbjct: 121 ITEVASATKRPDKVIGMHFFNPAPVMKLVEVIRGIATSQETFDAVKETSIAIGKDPVEVA 180

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDVCLS 243
           +  GF+VNRIL+PMINEA   L  G+A+ EDID  MKLG NHPMGPLEL D IGLD+CL+
Sbjct: 181 EAPGFVVNRILIPMINEAVGILAEGIASVEDIDKAMKLGANHPMGPLELGDFIGLDICLA 240

Query: 244 IMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRK 286
           IM VL+S  GD KY P  LL +YV AG LGRK G G Y Y K
Sbjct: 241 IMDVLYSETGDSKYRPHTLLKKYVRAGWLGRKSGKGFYDYSK 282

BLAST of Carg11334 vs. ExPASy Swiss-Prot
Match: P77851 (3-hydroxybutyryl-CoA dehydrogenase OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=hbd PE=3 SV=2)

HSP 1 Score: 269.6 bits (688), Expect = 4.1e-71
Identity = 141/278 (50.72%), Postives = 193/278 (69.42%), Query Frame = 0

Query: 7   IGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSREVGV 66
           I V+G G MGSGIAQ+ A  G +V L D D   + K   +I  ++QR V +  ++ +   
Sbjct: 4   ICVIGAGTMGSGIAQVFAQNGFEVILRDIDMKFVEKGFGTIEKNLQRNVDKGKITADEKN 63

Query: 67  DALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIPITR 126
           + L R++ +T+L++   AD ++EA +E+ D+K+++F ELD+I K   ILASNTSS+ IT 
Sbjct: 64  EILSRIRGTTNLEDAKEADFVVEAAIENMDLKKQIFKELDEICKMETILASNTSSLSITE 123

Query: 127 LASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICSQDYS 186
           +AS+T RPEKVIGMHF NP P+MKLVE+++G  TS++TF+ ++ LA ++ KT I  ++  
Sbjct: 124 IASATKRPEKVIGMHFFNPVPVMKLVEVIKGLKTSEQTFNVVRELALKVDKTPIEVKEAP 183

Query: 187 GFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDVCLSIMK 246
           GF+VNRIL+PMINEA   L  GVAT EDID  MKLG NHP+GPL L+DLIG DV L+IM 
Sbjct: 184 GFVVNRILIPMINEAIGILADGVATAEDIDEAMKLGANHPIGPLALSDLIGNDVVLAIMN 243

Query: 247 VLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYR 285
           VL+   GD KY P PLL + V  G LGRK G G + Y+
Sbjct: 244 VLYEEYGDSKYRPHPLLKKMVRGGLLGRKTGKGFFEYK 281

BLAST of Carg11334 vs. ExPASy TrEMBL
Match: A0A6J1E407 (uncharacterized protein LOC111430610 OS=Cucurbita moschata OX=3662 GN=LOC111430610 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 3.8e-160
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL
Sbjct: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SREVGVD LQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVDVLQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI
Sbjct: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKS SRL
Sbjct: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSKSRL 296

BLAST of Carg11334 vs. ExPASy TrEMBL
Match: A0A6J1J8K2 (uncharacterized protein LOC111482724 OS=Cucurbita maxima OX=3661 GN=LOC111482724 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.5e-156
Identity = 286/296 (96.62%), Postives = 291/296 (98.31%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIKVIGV+GGGQMGSGIAQLAAAYGHD+WLIDSDPAALTKATNSISSSIQRLVSRN+L
Sbjct: 1   MTEIKVIGVIGGGQMGSGIAQLAAAYGHDIWLIDSDPAALTKATNSISSSIQRLVSRNLL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SREVGVDALQRLKCSTDLKELHSADVIIEA+VESEDVKRKLFLELDKIAKPSAILASNTS
Sbjct: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAVVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRG DTSDETF AIKTLAERLGKTLI
Sbjct: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGVDTSDETFDAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNR+LMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYSGFIVNRLLMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVL SGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVY YRKLPKPEKS SRL
Sbjct: 241 CLSIMKVLQSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYDYRKLPKPEKSKSRL 296

BLAST of Carg11334 vs. ExPASy TrEMBL
Match: A0A0A0L6N5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G234000 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 266/296 (89.86%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIK IGVVGGGQMGSGIAQLAA YGH VWLIDSD AAL KAT+SISSSIQRLVSRN+L
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATYGHQVWLIDSDSAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SRE+G +ALQRLK STDLKELHSADVIIEAI+ESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 61  SREIGANALQRLKFSTDLKELHSADVIIEAILESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLAS+TTRPE+VIGMHFMNPPP+MKLVEIVRGADTS+ETF AIK LAERLGKT+I
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPVMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELAD IGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQYVDAGRLGRKR +GVY YRKLP+P+KS SRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRRIGVYDYRKLPEPDKSKSRL 296

BLAST of Carg11334 vs. ExPASy TrEMBL
Match: A0A5A7USC9 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold803G00200 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.5e-143
Identity = 266/296 (89.86%), Postives = 277/296 (93.58%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIK IGVVGGGQMGSGIAQLAA +GH VWLIDSDPAAL KAT+SISSSIQRLVSRN+L
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SRE+G  ALQRLK STDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLAS+TTRPE+VIGMHFMNPPPIMKLVEIVRGADTSDETF AIK LAERLGKT+I
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDIDTGMKLGTNHPMGPLELAD IGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQY DAGRLGRKRG+GVY YRK P   KS SRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

BLAST of Carg11334 vs. ExPASy TrEMBL
Match: A0A1S3C2Y7 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496074 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.5e-143
Identity = 266/296 (89.86%), Postives = 277/296 (93.58%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           MTEIK IGVVGGGQMGSGIAQLAA +GH VWLIDSDPAAL KAT+SISSSIQRLVSRN+L
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           SRE+G  ALQRLK STDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAK S+ILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SIPITRLAS+TTRPE+VIGMHFMNPPPIMKLVEIVRGADTSDETF AIK LAERLGKT+I
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDIDTGMKLGTNHPMGPLELAD IGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           CLSIMKVLHSGLGD+KYAPCPLLVQY DAGRLGRKRG+GVY YRK P   KS SRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

BLAST of Carg11334 vs. TAIR 10
Match: AT3G15290.1 (3-hydroxyacyl-CoA dehydrogenase family protein )

HSP 1 Score: 437.2 bits (1123), Expect = 1.1e-122
Identity = 217/285 (76.14%), Postives = 253/285 (88.77%), Query Frame = 0

Query: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNIL 60
           M E+K +GVVG GQMGSGIAQLAA  G DVWL+D+D  AL++AT +ISSS++R VS+ ++
Sbjct: 1   MAEMKSVGVVGAGQMGSGIAQLAATSGLDVWLMDADRDALSRATAAISSSVKRFVSKGLI 60

Query: 61  SREVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120
           S+EVG DA+ RL+ +++L++L SAD+I+EAIVESED+K+KLF +LD IAK SAILASNTS
Sbjct: 61  SKEVGDDAMHRLRLTSNLEDLCSADIIVEAIVESEDIKKKLFKDLDGIAKSSAILASNTS 120

Query: 121 SIPITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180
           SI ITRLAS+T RP +VIGMHFMNPPPIMKLVEI+RGADTS+ETF A K LAER GKT +
Sbjct: 121 SISITRLASATRRPSQVIGMHFMNPPPIMKLVEIIRGADTSEETFLATKVLAERFGKTTV 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240
           CSQDY+GF+VNRILMPMINEAF  LYTGVATKEDID+GMK GTNHPMGPLELADLIGLDV
Sbjct: 181 CSQDYAGFVVNRILMPMINEAFHTLYTGVATKEDIDSGMKHGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRK 286
           CLS+MKVLH GLGD KYAPCPLLVQYVDAGRLGRKRG+GVY YR+
Sbjct: 241 CLSVMKVLHEGLGDSKYAPCPLLVQYVDAGRLGRKRGVGVYDYRE 285

BLAST of Carg11334 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 143.7 bits (361), Expect = 2.4e-34
Identity = 92/293 (31.40%), Postives = 146/293 (49.83%), Query Frame = 0

Query: 4   IKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSRE 63
           IK + V+GGG MGSGIA         V L + +   L K   S+ ++++ LVSR  L+++
Sbjct: 309 IKKVAVIGGGLMGSGIATALLLSNIRVVLKEINSEFLMKGIKSVEANMKSLVSRGKLTQD 368

Query: 64  VGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSIP 123
               AL   K   D  E +  D++IEA++E+  +K+ +F E++K+  P  ILASNTS+I 
Sbjct: 369 KAGKALSLFKGVLDYTEFNDVDMVIEAVIENIQLKQNIFKEIEKVCSPHCILASNTSTID 428

Query: 124 ITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICSQ 183
           +  +   T   ++++G HF +P  +M L+EIVR  +TS +    +  + + + K  +   
Sbjct: 429 LDVIGEKTNSKDRIVGAHFFSPAHLMPLLEIVRSKNTSAQVILDLMAVGKAIKKVPVVVG 488

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDVCLS 243
           +  GF VNR   P    A      GV            G   P+GP +L DL G  + L+
Sbjct: 489 NCIGFAVNRTFFPYSQAAHMLANLGVDLFRIDSVITSFGL--PLGPFQLGDLAGHGIGLA 548

Query: 244 IMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGVYGYRKLPKPEKSNSRL 297
           +  +     GD  +   P+    + +GR G+  G G Y Y K  KP+   S L
Sbjct: 549 VGPIYAKVYGDRMFR-SPMTELLLKSGRNGKINGRGYYIYEKGSKPKPDPSVL 598

BLAST of Carg11334 vs. TAIR 10
Match: AT3G06860.1 (multifunctional protein 2 )

HSP 1 Score: 136.7 bits (343), Expect = 3.0e-32
Identity = 89/291 (30.58%), Postives = 148/291 (50.86%), Query Frame = 0

Query: 3   EIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNILSR 62
           +IK + ++GGG MGSGIA       + V L + +   L      + +++Q  V +  +S+
Sbjct: 311 KIKKVAIIGGGLMGSGIATALILSNYPVILKEVNEKFLEAGIGRVKANLQSRVRKGSMSQ 370

Query: 63  EVGVDALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTSSI 122
           E     +  LK S D +     D++IEA++E+  +K+++F +L+K      ILASNTS+I
Sbjct: 371 EKFEKTMSLLKGSLDYESFRDVDMVIEAVIENISLKQQIFADLEKYCPQHCILASNTSTI 430

Query: 123 PITRLASSTTRPEKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLICS 182
            + ++   T   ++++G HF +P  IM L+EIVR   TS +    +  + +++ KT +  
Sbjct: 431 DLNKIGERTKSQDRIVGAHFFSPAHIMPLLEIVRTNHTSAQVIVDLLDVGKKIKKTPVVV 490

Query: 183 QDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGM-KLGTNHPMGPLELADLIGLDVC 242
            + +GF VNR+  P    A F +  G A    ID  + K G   PMGP  L DL+G  V 
Sbjct: 491 GNCTGFAVNRMFFPYTQAAMFLVECG-ADPYLIDRAISKFGM--PMGPFRLCDLVGFGVA 550

Query: 243 LSIMKVLHSGLGDDKYAP--CPLLVQYVDAGRLGRKRGLGVYGYRKLPKPE 291
           ++          +  Y     PL+ +   AG   RK G  +Y  ++  KP+
Sbjct: 551 IATATQFIENFSERTYKSMIIPLMQEDKRAGEATRK-GFYLYDDKRKAKPD 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015024.15.4e-161100.00hbdA [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022922687.17.8e-16099.32uncharacterized protein LOC111430610 [Cucurbita moschata] >XP_022922688.1 unchar... [more]
XP_023553026.19.5e-15897.64uncharacterized protein LOC111810545 [Cucurbita pepo subsp. pepo][more]
XP_022984420.13.1e-15696.62uncharacterized protein LOC111482724 [Cucurbita maxima][more]
KAG6577002.16.4e-154100.00Protein DETOXIFICATION 19, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q452235.2e-8255.523-hydroxybutyryl-CoA dehydrogenase OS=Bradyrhizobium diazoefficiens (strain JCM ... [more]
P458562.9e-7753.00Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Bacillus subtilis (strain 168) OX... [more]
Q9RVG11.8e-7453.62Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Deinococcus radiodurans (strain A... [more]
P520413.4e-7351.423-hydroxybutyryl-CoA dehydrogenase OS=Clostridium acetobutylicum (strain ATCC 82... [more]
P778514.1e-7150.723-hydroxybutyryl-CoA dehydrogenase OS=Thermoanaerobacterium thermosaccharolyticu... [more]
Match NameE-valueIdentityDescription
A0A6J1E4073.8e-16099.32uncharacterized protein LOC111430610 OS=Cucurbita moschata OX=3662 GN=LOC1114306... [more]
A0A6J1J8K21.5e-15696.62uncharacterized protein LOC111482724 OS=Cucurbita maxima OX=3661 GN=LOC111482724... [more]
A0A0A0L6N51.0e-14489.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G234000 PE=3 SV=1[more]
A0A5A7USC95.5e-14389.863-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3C2Y75.5e-14389.863-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496074 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G15290.11.1e-12276.143-hydroxyacyl-CoA dehydrogenase family protein [more]
AT4G29010.12.4e-3431.40Enoyl-CoA hydratase/isomerase family [more]
AT3G06860.13.0e-3230.58multifunctional protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0226943-hydroxyacyl-CoA dehydrogenasePIRSFPIRSF000105HCDHcoord: 1..295
e-value: 9.7E-90
score: 298.9
IPR0061763-hydroxyacyl-CoA dehydrogenase, NAD bindingPFAMPF027373HCDH_Ncoord: 7..184
e-value: 4.1E-63
score: 212.5
IPR0061083-hydroxyacyl-CoA dehydrogenase, C-terminalPFAMPF007253HCDHcoord: 187..283
e-value: 5.5E-35
score: 119.8
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 190..295
e-value: 1.8E-32
score: 114.2
NoneNo IPR availableGENE3D3.40.50.720coord: 1..189
e-value: 2.8E-67
score: 228.5
NoneNo IPR availablePANTHERPTHR480753-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEINcoord: 4..289
NoneNo IPR availablePANTHERPTHR48075:SF53-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEINcoord: 4..289
IPR0061803-hydroxyacyl-CoA dehydrogenase, conserved sitePROSITEPS000673HCDHcoord: 184..209
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 187..285
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 1..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11334-RACarg11334-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0070403 NAD+ binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function GO:0016491 oxidoreductase activity