Carg11163 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11163
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	PMEI domain-containing protein
Location	Carg_Chr14: 5022425 .. 5023126 (-)
RNA-Seq Expression	Carg11163
Synteny	Carg11163

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTCATCAACAATGTCTTCCACCGCCTCCTCCTCCTCCTCCTCCTCCTCGCCCTCTCCTCTCTTTTCTCCTCCCCCGCCATTGCCATTTGCGTTCCTCGAAACACCTCCCTCTTTCAATTCTCTACTCTTCCACTTCCTACACCAAGCACCCCGCCGGTTTACATTTCGCAGTCGATATCTCGAAAACGAACGCCGAAGTCCGCGGGCAAATCGAGTTCGAAATCGGCGTCGTCGTCGTCGACGGAACCGACCGAATTGGTGCAGATCTGCGATGTGACGAGCAATCCAAAGCTGTGTGAATCCTCGATTTCATCTAGCATCCATGGATCAACGGTGAATCCTGTTTCTGCACTGAAAACGGAAATTCAGCTATCGATTAAAGAGGTGGAGGACGCAATTGCGGTATTGAAAAAACTGGACAAAGAATCTGGTTTAACGAATGCGGAAGACTCGTGCTACGATACTTGCCTGGAGAATTTCGATATGGCGATTGATGATCTACGGACCGCAACGGAAGCGATCGACGTGAATGACGTTGGTAGAATGGAGAGCGCACTGACGGCCGTATTGACGGATCTGGTGACTTGCGATGATACTTTTCTGGAGATGGGAGTGGATTCTCCATTGGACGATCTTAGTATCAAAATGAACAAGTACGCCAGTAACTGCTTGGCCATTTCCAAATTGCTCCTGTGA

mRNA sequence

ATGGAGGTCATCAACAATGTCTTCCACCGCCTCCTCCTCCTCCTCCTCCTCCTCGCCCTCTCCTCTCTTTTCTCCTCCCCCGCCATTGCCATTTGCGTTCCTCGAAACACCTCCCTCTTTCAATTCTCTACTCTTCCACTTCCTACACCAAGCACCCCGCCGGTTTACATTTCGCAGTCGATATCTCGAAAACGAACGCCGAAGTCCGCGGGCAAATCGAGTTCGAAATCGGCGTCGTCGTCGTCGACGGAACCGACCGAATTGGTGCAGATCTGCGATGTGACGAGCAATCCAAAGCTGTGTGAATCCTCGATTTCATCTAGCATCCATGGATCAACGGTGAATCCTGTTTCTGCACTGAAAACGGAAATTCAGCTATCGATTAAAGAGGTGGAGGACGCAATTGCGGTATTGAAAAAACTGGACAAAGAATCTGGTTTAACGAATGCGGAAGACTCGTGCTACGATACTTGCCTGGAGAATTTCGATATGGCGATTGATGATCTACGGACCGCAACGGAAGCGATCGACGTGAATGACGTTGGTAGAATGGAGAGCGCACTGACGGCCGTATTGACGGATCTGGTGACTTGCGATGATACTTTTCTGGAGATGGGAGTGGATTCTCCATTGGACGATCTTAGTATCAAAATGAACAAGTACGCCAGTAACTGCTTGGCCATTTCCAAATTGCTCCTGTGA

Coding sequence (CDS)

ATGGAGGTCATCAACAATGTCTTCCACCGCCTCCTCCTCCTCCTCCTCCTCCTCGCCCTCTCCTCTCTTTTCTCCTCCCCCGCCATTGCCATTTGCGTTCCTCGAAACACCTCCCTCTTTCAATTCTCTACTCTTCCACTTCCTACACCAAGCACCCCGCCGGTTTACATTTCGCAGTCGATATCTCGAAAACGAACGCCGAAGTCCGCGGGCAAATCGAGTTCGAAATCGGCGTCGTCGTCGTCGACGGAACCGACCGAATTGGTGCAGATCTGCGATGTGACGAGCAATCCAAAGCTGTGTGAATCCTCGATTTCATCTAGCATCCATGGATCAACGGTGAATCCTGTTTCTGCACTGAAAACGGAAATTCAGCTATCGATTAAAGAGGTGGAGGACGCAATTGCGGTATTGAAAAAACTGGACAAAGAATCTGGTTTAACGAATGCGGAAGACTCGTGCTACGATACTTGCCTGGAGAATTTCGATATGGCGATTGATGATCTACGGACCGCAACGGAAGCGATCGACGTGAATGACGTTGGTAGAATGGAGAGCGCACTGACGGCCGTATTGACGGATCTGGTGACTTGCGATGATACTTTTCTGGAGATGGGAGTGGATTCTCCATTGGACGATCTTAGTATCAAAATGAACAAGTACGCCAGTAACTGCTTGGCCATTTCCAAATTGCTCCTGTGA

Protein sequence

MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQSISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL

Homology

[Close]

BLAST of Carg11163 vs. NCBI nr
Match: KAG7018024.1 (hypothetical protein SDJN02_19890, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 425.6 bits (1093), Expect = 2.7e-115
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60
           MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS
Sbjct: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60

Query: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120
           ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL
Sbjct: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120

Query: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180
           KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND
Sbjct: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180

Query: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 234
           VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL
Sbjct: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 233

[Close]

BLAST of Carg11163 vs. NCBI nr
Match: KAG6581304.1 (hypothetical protein SDJN03_21306, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 420.6 bits (1080), Expect = 8.7e-114
Identity = 233/235 (99.15%), Postives = 233/235 (99.15%), Query Frame = 0


Query: 1   MEVINNVFHR--LLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYIS 60
           MEVINNVFHR  LLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYIS
Sbjct: 1   MEVINNVFHRLLLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYIS 60

Query: 61  QSISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVS 120
           QSISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVS
Sbjct: 61  QSISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVS 120

Query: 121 ALKTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDV 180
           ALKTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDV
Sbjct: 121 ALKTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDV 180

Query: 181 NDVGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 234
           NDVGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL
Sbjct: 181 NDVGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 235

[Close]

BLAST of Carg11163 vs. NCBI nr
Match: XP_022934390.1 (uncharacterized protein LOC111441579 [Cucurbita moschata])

HSP 1 Score: 416.8 bits (1070), Expect = 1.3e-112
Identity = 230/233 (98.71%), Postives = 230/233 (98.71%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60
           MEVINNVFHR  LLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS
Sbjct: 1   MEVINNVFHR--LLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60

Query: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120
           ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL
Sbjct: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120

Query: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180
           KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND
Sbjct: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180

Query: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 234
           VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYA NCLAISKLLL
Sbjct: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYAINCLAISKLLL 231

[Close]

BLAST of Carg11163 vs. NCBI nr
Match: XP_023529115.1 (uncharacterized protein LOC111791850 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.1 bits (980), Expect = 3.4e-102
Identity = 214/233 (91.85%), Postives = 220/233 (94.42%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60
           MEV+NNVFHR    LLLLALSSLFSSP IAICVPRNTSLFQFSTLPLPTPSTPPVYISQS
Sbjct: 1   MEVVNNVFHR---FLLLLALSSLFSSPGIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60

Query: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120
           ISRKRTPKS GKSSSKSA SSS EPTELVQICDVTSNPKLCESSISSSIH STVNPVSAL
Sbjct: 61  ISRKRTPKSEGKSSSKSA-SSSREPTELVQICDVTSNPKLCESSISSSIHESTVNPVSAL 120

Query: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180
           KTEIQLSIKEVE+AIAVLKKL KESG T AED+CYDTCLENFDMAIDDLRTATEAIDVND
Sbjct: 121 KTEIQLSIKEVENAIAVLKKLYKESGSTKAEDACYDTCLENFDMAIDDLRTATEAIDVND 180

Query: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 234
           VGRM+SALTAVLTDLVTCDDTF+EMGVDSPLD LSIKM+KYASNCLAISKLLL
Sbjct: 181 VGRMQSALTAVLTDLVTCDDTFVEMGVDSPLDYLSIKMSKYASNCLAISKLLL 229

[Close]

BLAST of Carg11163 vs. NCBI nr
Match: XP_022983975.1 (uncharacterized protein LOC111482436 [Cucurbita maxima])

HSP 1 Score: 365.9 bits (938), Expect = 2.5e-97
Identity = 205/233 (87.98%), Postives = 214/233 (91.85%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60
           ME+INNVFH  L L LLLALSSLFSSPAIAICVPRN SLFQ  T+PLPTPSTPPVYISQS
Sbjct: 32  MELINNVFHLHLHLHLLLALSSLFSSPAIAICVPRNISLFQSPTIPLPTPSTPPVYISQS 91

Query: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120
           ++RKRT KS GKSSSK  S SS EP ELVQICDVTSNPKLC+SSISSSIH STVNPVSAL
Sbjct: 92  VARKRTQKSEGKSSSK--SGSSREPIELVQICDVTSNPKLCQSSISSSIHESTVNPVSAL 151

Query: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180
           KTEI+ SIKEVE+AIAVLKKLDKESG T AEDSCYDTCLENFDMAI+DLRTATEAIDVND
Sbjct: 152 KTEIEQSIKEVENAIAVLKKLDKESGSTKAEDSCYDTCLENFDMAIEDLRTATEAIDVND 211

Query: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 234
           VGRMESALTAVLTDLVTCDDTF+EMGVDSPLD LSIKMNKYASNCLAISKLLL
Sbjct: 212 VGRMESALTAVLTDLVTCDDTFVEMGVDSPLDHLSIKMNKYASNCLAISKLLL 262

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BLAST of Carg11163 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 5.3e-05
Identity = 43/166 (25.90%), Postives = 82/166 (49.40%), Query Frame = 0


Query: 78  ASSSSTEPTELVQI-CDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIA 137
           A++S+    + ++  C +T+ P +CE S+S+       NP     T +Q+S+   + A  
Sbjct: 18  AAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQT 77

Query: 138 VLKKLDKESGLTNAEDSCYDTCLENFDMAIDDL-RTATEAIDV-----ND----VGRMES 197
            +K+L+K  GL   + +    CLE  + ++D + R+  E  ++     ND    +  +E+
Sbjct: 78  FMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVET 137

Query: 198 ALTAVLTDLVTCDDTFLEMGVDSPLDD----LSIKMNKYASNCLAI 229
            ++A LTD  TC D F   G+D  + +      + + +  SN LA+
Sbjct: 138 WVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALAL 183

[Close]

BLAST of Carg11163 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.4e-04
Identity = 45/165 (27.27%), Postives = 72/165 (43.64%), Query Frame = 0


Query: 83  TEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIAVLKKLD 142
           T    L  +C VT  P  C SSIS     +T +P +  +  +Q+ I E+     + KKL 
Sbjct: 266 TPAASLRNVCSVTRYPASCVSSISKLPSSNTTDPEALFRLSLQVVINELNSIAGLPKKLA 325

Query: 143 KESG---LTNAEDSCYDTCLENFDMAIDDLRTATEAID------VNDVGRMESALTAVLT 202
           +E+    L ++   C D   +  D+  D + T  E  D       + +  +++ L+A +T
Sbjct: 326 EETDDERLKSSLSVCGDVFNDAIDIVNDTISTMEEVGDGKKILKSSTIDEIQTWLSAAVT 385

Query: 203 DLVTCDDTFLEMG------VDSP----LDDLSIKMNKYASNCLAI 229
           D  TC D   E+        +SP    L    +   K+ SN LAI
Sbjct: 386 DHDTCLDALDELSQNKTEYANSPISLKLKSAMVNSRKFTSNSLAI 430

[Close]

BLAST of Carg11163 vs. ExPASy TrEMBL
Match: A0A6J1F7J2 (uncharacterized protein LOC111441579 OS=Cucurbita moschata OX=3662 GN=LOC111441579 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.1e-113
Identity = 230/233 (98.71%), Postives = 230/233 (98.71%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60
           MEVINNVFHR  LLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS
Sbjct: 1   MEVINNVFHR--LLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60

Query: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120
           ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL
Sbjct: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120

Query: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180
           KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND
Sbjct: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180

Query: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 234
           VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYA NCLAISKLLL
Sbjct: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYAINCLAISKLLL 231

[Close]

BLAST of Carg11163 vs. ExPASy TrEMBL
Match: A0A6J1J7E6 (uncharacterized protein LOC111482436 OS=Cucurbita maxima OX=3661 GN=LOC111482436 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.2e-97
Identity = 205/233 (87.98%), Postives = 214/233 (91.85%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLPTPSTPPVYISQS 60
           ME+INNVFH  L L LLLALSSLFSSPAIAICVPRN SLFQ  T+PLPTPSTPPVYISQS
Sbjct: 32  MELINNVFHLHLHLHLLLALSSLFSSPAIAICVPRNISLFQSPTIPLPTPSTPPVYISQS 91

Query: 61  ISRKRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSAL 120
           ++RKRT KS GKSSSK  S SS EP ELVQICDVTSNPKLC+SSISSSIH STVNPVSAL
Sbjct: 92  VARKRTQKSEGKSSSK--SGSSREPIELVQICDVTSNPKLCQSSISSSIHESTVNPVSAL 151

Query: 121 KTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVND 180
           KTEI+ SIKEVE+AIAVLKKLDKESG T AEDSCYDTCLENFDMAI+DLRTATEAIDVND
Sbjct: 152 KTEIEQSIKEVENAIAVLKKLDKESGSTKAEDSCYDTCLENFDMAIEDLRTATEAIDVND 211

Query: 181 VGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 234
           VGRMESALTAVLTDLVTCDDTF+EMGVDSPLD LSIKMNKYASNCLAISKLLL
Sbjct: 212 VGRMESALTAVLTDLVTCDDTFVEMGVDSPLDHLSIKMNKYASNCLAISKLLL 262

[Close]

BLAST of Carg11163 vs. ExPASy TrEMBL
Match: A0A5A7TT72 (Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001500 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 8.6e-51
Identity = 138/269 (51.30%), Postives = 167/269 (62.08%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLP------------ 60
           ME+INN  +    + LLLA SS+F S AIAICVP N ++    +  +P            
Sbjct: 1   MELINNNINN---VFLLLAFSSVFFS-AIAICVPLNINITDVESTAIPPLLEQTVSPQVA 60

Query: 61  ---------TPSTPPVYISQSISRKRTPKSAGKSSSKS---------------ASSSSTE 120
                    T S PP   S   S      S   S  ++               ++++ST 
Sbjct: 61  PDQSPPAVTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLHSDGLISTAASTV 120

Query: 121 PTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIAVLKKLDKE 180
            T LV+ICDVTSNP+LC++SISS I G+ V+P SALKTEI+ SIKEV  AIA L  L K+
Sbjct: 121 KTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIATLNSLRKD 180

Query: 181 SGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAVLTDLVTCDDTFLE 234
           S  +  E +CYDTCLENFDMAI+DL+   E+ID  D GRMES LTAVLTDL TCDDTF E
Sbjct: 181 SSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTTCDDTFAE 240

[Close]

BLAST of Carg11163 vs. ExPASy TrEMBL
Match: A0A0A0KWP8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 8.6e-51
Identity = 141/282 (50.00%), Postives = 172/282 (60.99%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTS---------LFQFSTLPLPTP- 60
           ME+INN  H    + LLLA SS+FS  A+A+CVPRNTS         LF+ +  P   P 
Sbjct: 1   MELINNSIHN---VFLLLAFSSVFSF-AVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQ 60

Query: 61  ----STPPVYISQ---------SISRKRTPKSAGKSSSKSASSSSTE------------- 120
               S P V +S+         S S+  +   + ++ S+ A+S + E             
Sbjct: 61  VAPQSPPAVTVSEEPEAASPETSPSQPSSASESEEAESEEAASETEEDDLGLLGGGLDQL 120

Query: 121 -------------PTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEV 180
                         T LV ICDVTSNP+LC++SISS I G+ V+P SALKTEI  SIKEV
Sbjct: 121 HSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIDESIKEV 180

Query: 181 EDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAV 234
             AIA L  L K+S  +  E +CYDTCLENF+MAIDDL+   E+I+  D GRMES LTAV
Sbjct: 181 AKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAV 240

[Close]

BLAST of Carg11163 vs. ExPASy TrEMBL
Match: A0A1S3BYC7 (pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 8.6e-51
Identity = 138/269 (51.30%), Postives = 167/269 (62.08%), Query Frame = 0


Query: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLP------------ 60
           ME+INN  +    + LLLA SS+F S AIAICVP N ++    +  +P            
Sbjct: 1   MELINNNINN---VFLLLAFSSVFFS-AIAICVPLNINITDVESTAIPPLLEQTVSPQVA 60

Query: 61  ---------TPSTPPVYISQSISRKRTPKSAGKSSSKS---------------ASSSSTE 120
                    T S PP   S   S      S   S  ++               ++++ST 
Sbjct: 61  PDQSPPAVTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLHSDGLISTAASTV 120

Query: 121 PTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIAVLKKLDKE 180
            T LV+ICDVTSNP+LC++SISS I G+ V+P SALKTEI+ SIKEV  AIA L  L K+
Sbjct: 121 KTNLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIATLNSLRKD 180

Query: 181 SGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAVLTDLVTCDDTFLE 234
           S  +  E +CYDTCLENFDMAI+DL+   E+ID  D GRMES LTAVLTDL TCDDTF E
Sbjct: 181 SSASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTTCDDTFAE 240

[Close]

BLAST of Carg11163 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-13
Identity = 78/246 (31.71%), Postives = 113/246 (45.93%), Query Frame = 0


Query: 22  SLFSSPAIA------ICVPRNTSLFQFSTLPLP---------TPSTPPVYISQSISRKRT 81
           S FS+PA A      I  P N+    ++  P+          +P  PPV I    S   +
Sbjct: 31  SPFSTPAPAPEVMKPIASPANSPAEIYTDSPVASPEAPADSFSPKNPPVEI-DIYSPAAS 90

Query: 82  PKSAGKSSSKSASSSSTEPT-------------------ELVQICDVTSNPKLCESSISS 141
           P++   S    A SSS  P+                   E+  IC  T NP LCESS+S 
Sbjct: 91  PEAPVASPEAPAESSSAAPSGIKVSSSPKSLLNPPLLSPEIKTICGKTDNPPLCESSVSP 150

Query: 142 SIHGS-TVNPVSALKTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAI 201
            +      N  S L   IQ SI   + A+A+++K+D       A D     C E +D A+
Sbjct: 151 LLTPQLKPNTSSVLILAIQASITATKAAMAIVEKVD-------ASD-----CQELYDDAV 210

Query: 202 DDLRTATEAIDVNDVGRMESALTAVLTDLVTCDDTFLEMGVDSPLDDLSIKMNKYASNCL 233
            +L  A  A+  +D+  + + L+A +TD  TC+D F E G  +PL  ++ K+ K  SNCL
Sbjct: 211 VNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAYVADKLTKMVSNCL 263

[Close]

BLAST of Carg11163 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-09
Identity = 35/118 (29.66%), Postives = 61/118 (51.69%), Query Frame = 0


Query: 114 VNPVSALKTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTAT 173
           + P   ++ E +  +K+    +   K++ ++             C+EN+D  I  L  A 
Sbjct: 160 IAPKDVMRMEAESLLKKANATLDYAKRVVEDRKTPMPVKEAMSVCVENYDSLISGLEDAK 219

Query: 174 EAIDVNDVGRMESALTAVLTDLVTCDDTFLEM-GVDSPLDDLSIKMNKYASNCLAISK 231
            A++  D GR++S L+A ++D+ TC D F+++  VDSP   L   M K  SN LA+S+
Sbjct: 220 MAMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDSPTASLDDLMKKLCSNVLAMSQ 277

[Close]

BLAST of Carg11163 vs. TAIR 10
Match: AT1G02550.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.6e-07
Identity = 65/238 (27.31%), Postives = 104/238 (43.70%), Query Frame = 0


Query: 13  LLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLPLP--TPSTPPVYISQSISR------- 72
           LL   LAL SL SSP+ +    R  S    S  P P   P+T         S        
Sbjct: 7   LLCFSLALFSLLSSPSSS---TRIISSIVPSAAPSPAVAPTTDGDVDENDFSAFTQWNIL 66

Query: 73  -----KRTPKSAGKSSSKSASSSSTEPTELVQICDVTSNPKLCESSISSSIHG---STVN 132
                K T K+    S  + SS    P  +  +C  T     C  SI   +         
Sbjct: 67  NLTDLKSTFKNLPDFSKLNISSLHVSPA-VGSVCSNTDYAAECIVSILPLLRDFRKFEPK 126

Query: 133 PVSALKTEIQLSIKEVEDAIAVLKKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEA 192
           P+  L+ E+    ++    + + K+L  +           D C++N++  +DDL+ A+ A
Sbjct: 127 PIDVLRMEMSALYEKANATLDLAKRLIVDKSTPRDVADVLDLCVDNYESLLDDLKDASVA 186

Query: 193 IDVNDVGRMESALTAVLTDLVTCDDTFLEMG-VDSPLDDLSIKMNKYASNCLAISKLL 233
           +D  D  R+ES ++A + D+VTC D F E   ++SP+ ++   + K  SN LAIS+++
Sbjct: 187 VDDGDFERLESVVSAAIADVVTCSDAFAESSELESPMANVDDFLKKLCSNVLAISQMI 240

[Close]

BLAST of Carg11163 vs. TAIR 10
Match: AT1G10770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 9.9e-07
Identity = 38/149 (25.50%), Postives = 70/149 (46.98%), Query Frame = 0


Query: 86  TELVQICDVTSNPKLCESSIS--SSIHGSTVNPVSALKTEIQLSIKEVEDAIAVLKKLDK 145
           + ++ IC  T+ P LC   +   +S   +T   + AL+ + +L++               
Sbjct: 32  SRMINICSHTAYPSLCRPLVKRVTSPRKATHRTIQALEAKTKLAL--------------A 91

Query: 146 ESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAVLTDLVTCDDTFL 205
           E+      +    TC E    A+ +L +A ++I   DV  M + LTA ++D   C D F+
Sbjct: 92  ETARFKNGNQAVSTCYETLGDAVYNLASARKSIRKRDVPAMNTYLTAAVSDYGACVDGFI 151

Query: 206 EMGVDSPLDDLSIKMNKYASNCLAISKLL 233
           E    + + +  + + K +SNCLA+S L+
Sbjct: 152 ETQQVNAIQNAVVDLRKISSNCLALSTLI 166

[Close]

BLAST of Carg11163 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.2e-06
Identity = 47/157 (29.94%), Postives = 71/157 (45.22%), Query Frame = 0


Query: 77  SASSSSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIA 136
           +A+SS      L   C V+  P LC S+I         N  +A    I+  +K    A  
Sbjct: 14  AATSSQALSISLADPCTVSDFPALCRSTIKGQ-----TNVNAATDVAIKELMKRTRQAKK 73

Query: 137 VL-KKLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAVLTDL 196
           +  K+L ++ G+         TCL NF+ A D+L  A   I  ND   +   L+A LTD 
Sbjct: 74  IAEKELKRDGGVA--------TCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDY 133

Query: 197 VTCDDTFLEMGVDSPLDDLSIKMNKYASNCLAISKLL 233
            TC D+  E    + +   +  + + A NCLA+S L+
Sbjct: 134 DTCSDSMKETKEVNVIYKSAGVLYRMADNCLALSTLV 157

The following BLAST results are available for this feature:

BLAST of Carg11163 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018024.1	2.7e-115	100.00	hypothetical protein SDJN02_19890, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6581304.1	8.7e-114	99.15	hypothetical protein SDJN03_21306, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022934390.1	1.3e-112	98.71	uncharacterized protein LOC111441579 [Cucurbita moschata]	[more]
XP_023529115.1	3.4e-102	91.85	uncharacterized protein LOC111791850 [Cucurbita pepo subsp. pepo]	[more]
XP_022983975.1	2.5e-97	87.98	uncharacterized protein LOC111482436 [Cucurbita maxima]	[more]

BLAST of Carg11163 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	5.3e-05	25.90	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9LUL8	3.4e-04	27.27	Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg11163 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F7J2	6.1e-113	98.71	uncharacterized protein LOC111441579 OS=Cucurbita moschata OX=3662 GN=LOC1114415...	[more]
A0A6J1J7E6	1.2e-97	87.98	uncharacterized protein LOC111482436 OS=Cucurbita maxima OX=3661 GN=LOC111482436...	[more]
A0A5A7TT72	8.6e-51	51.30	Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0KWP8	8.6e-51	50.00	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 S...	[more]
A0A1S3BYC7	8.6e-51	51.30	pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1	[more]

BLAST of Carg11163 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G36659.1	2.4e-13	31.71	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47340.1	1.6e-09	29.66	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G02550.1	2.6e-07	27.31	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G10770.1	9.9e-07	25.50	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G23350.1	2.2e-06	29.94	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 84..228 e-value: 1.7E-24 score: 97.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 90..228 e-value: 2.9E-21 score: 76.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 79..232 e-value: 9.6E-24 score: 81.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 86..232 e-value: 1.2E-29 score: 105.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 90..232
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 56..84
None	No IPR available	PANTHER	PTHR31707:SF119	INVERTASE-RELATED	coord: 19..227
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 19..227
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 87..232 e-value: 1.02267E-43 score: 142.499

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11163-RA	Carg11163-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity