Carg10953 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10953
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionUnknown protein
LocationCarg_Chr08: 6457341 .. 6457691 (+)
RNA-Seq ExpressionCarg10953
SyntenyCarg10953
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAATCGCAGAGACGACCCTCCGGCACCGCCGCCCGGAAGCAGCAGCCAAGCCGCCTGCAGCGCCGGGCTCCAGCCTCCCTCCAAATCAGCCGCTCCGATTGGAAGGTCGCCATCCCTCTCCTATCGCCACTCGTATCGCCATCGTCGCCGACAGAGATCCAAAATTGGACCGCCGATTCAGGATCGATTTCTTCTTCTTCTTCTCATCATCATCATCATCATCATCATCATCATAAAGAATCGGAGCCGGAGAAGCCGCCAGTTTTTAAGAAATGGCAGCATCCGGCGGCGCCGTTTCATTACGATTCGGGTTCTAGGGCTCCCAGAGCATTCGTACCCGTTTAG

mRNA sequence

ATGGCCGAATCGCAGAGACGACCCTCCGGCACCGCCGCCCGGAAGCAGCAGCCAAGCCGCCTGCAGCGCCGGGCTCCAGCCTCCCTCCAAATCAGCCGCTCCGATTGGAAGGTCGCCATCCCTCTCCTATCGCCACTCGTATCGCCATCGTCGCCGACAGAGATCCAAAATTGGACCGCCGATTCAGGATCGATTTCTTCTTCTTCTTCTCATCATCATCATCATCATCATCATCATCATAAAGAATCGGAGCCGGAGAAGCCGCCAGTTTTTAAGAAATGGCAGCATCCGGCGGCGCCGTTTCATTACGATTCGGGTTCTAGGGCTCCCAGAGCATTCGTACCCGTTTAG

Coding sequence (CDS)

ATGGCCGAATCGCAGAGACGACCCTCCGGCACCGCCGCCCGGAAGCAGCAGCCAAGCCGCCTGCAGCGCCGGGCTCCAGCCTCCCTCCAAATCAGCCGCTCCGATTGGAAGGTCGCCATCCCTCTCCTATCGCCACTCGTATCGCCATCGTCGCCGACAGAGATCCAAAATTGGACCGCCGATTCAGGATCGATTTCTTCTTCTTCTTCTCATCATCATCATCATCATCATCATCATCATAAAGAATCGGAGCCGGAGAAGCCGCCAGTTTTTAAGAAATGGCAGCATCCGGCGGCGCCGTTTCATTACGATTCGGGTTCTAGGGCTCCCAGAGCATTCGTACCCGTTTAG

Protein sequence

MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTADSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV
Homology
BLAST of Carg10953 vs. NCBI nr
Match: KAG6593848.1 (putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026180.1 putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 228.4 bits (581), Expect = 3.1e-56
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60
           MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA
Sbjct: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60

Query: 61  DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
           DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV
Sbjct: 61  DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 116

BLAST of Carg10953 vs. NCBI nr
Match: XP_023000559.1 (uncharacterized protein At4g14450, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 216.5 bits (550), Expect = 1.2e-52
Identity = 113/116 (97.41%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60
           MAESQRR SG AARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA
Sbjct: 1   MAESQRRASGAAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60

Query: 61  DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
           DSGSISSSSS HHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV
Sbjct: 61  DSGSISSSSS-HHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 115

BLAST of Carg10953 vs. NCBI nr
Match: XP_022930471.1 (uncharacterized protein At4g14450, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 216.1 bits (549), Expect = 1.6e-52
Identity = 113/116 (97.41%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60
           MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA
Sbjct: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60

Query: 61  DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
           DSGSISSSSS   HHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV
Sbjct: 61  DSGSISSSSS---HHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 113

BLAST of Carg10953 vs. NCBI nr
Match: XP_023515028.1 (uncharacterized protein At4g14450, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 211.1 bits (536), Expect = 5.2e-51
Identity = 109/116 (93.97%), Postives = 111/116 (95.69%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60
           MAESQRRPS TA+RKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA
Sbjct: 1   MAESQRRPSATASRKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60

Query: 61  DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
           DSG+ISSS    HHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV
Sbjct: 61  DSGAISSS----HHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 112

BLAST of Carg10953 vs. NCBI nr
Match: XP_038875425.1 (uncharacterized protein At4g14450, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 168.7 bits (426), Expect = 3.0e-38
Identity = 94/117 (80.34%), Postives = 98/117 (83.76%), Query Frame = 0

Query: 1   MAESQRRPSGTAAR-KQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWT 60
           MA+S RR SG + R  QQPSRLQRRAPASLQI+RSDWKVAIPLLSPLVSPSSP +IQNWT
Sbjct: 1   MADSHRRTSGASGRHPQQPSRLQRRAPASLQINRSDWKVAIPLLSPLVSPSSPKDIQNWT 60

Query: 61  ADSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
            DS   SSSSS      HHHHKESEPEKPPVFKKWQHPAAPFHYDSG RAPR FVPV
Sbjct: 61  PDSREASSSSS-----QHHHHKESEPEKPPVFKKWQHPAAPFHYDSGPRAPR-FVPV 111

BLAST of Carg10953 vs. ExPASy Swiss-Prot
Match: Q6NN02 (Uncharacterized protein At4g14450, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g14450 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 3.1e-06
Identity = 48/117 (41.03%), Postives = 62/117 (52.99%), Query Frame = 0

Query: 5   QRRPSGTAARKQQPSRLQRRAPASL--QISRSDWKVAIPLLSPLVSPSSPTEIQNWTADS 64
           QR  S  A+ ++Q S+LQRRAP+ +    S S+W VAIPLLSPL               +
Sbjct: 24  QRTSSSAASNERQLSQLQRRAPSLMIKPTSFSNWNVAIPLLSPL---------------A 83

Query: 65  GSISSSSSHHH----HHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVP 116
            S++SS    H     +      E E +K PVFKKWQHPA+PF Y+     P  FVP
Sbjct: 84  PSLTSSFDQSHVPPPQNKTEIPVEEEVKKTPVFKKWQHPASPFCYE-----PTTFVP 120

BLAST of Carg10953 vs. ExPASy TrEMBL
Match: A0A6J1KKB8 (uncharacterized protein At4g14450, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111494801 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 6.0e-53
Identity = 113/116 (97.41%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60
           MAESQRR SG AARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA
Sbjct: 1   MAESQRRASGAAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60

Query: 61  DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
           DSGSISSSSS HHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV
Sbjct: 61  DSGSISSSSS-HHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 115

BLAST of Carg10953 vs. ExPASy TrEMBL
Match: A0A6J1ER11 (uncharacterized protein At4g14450, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436912 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.8e-53
Identity = 113/116 (97.41%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60
           MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA
Sbjct: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWTA 60

Query: 61  DSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
           DSGSISSSSS   HHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV
Sbjct: 61  DSGSISSSSS---HHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 113

BLAST of Carg10953 vs. ExPASy TrEMBL
Match: A0A1S3CDB1 (uncharacterized protein At4g14450, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103499403 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 9.3e-38
Identity = 97/128 (75.78%), Postives = 100/128 (78.12%), Query Frame = 0

Query: 1   MAESQRRPSGTAAR-KQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWT 60
           MA+S RR S T  R  QQPSRLQRRAPASLQI+RSDWKVAIPLLSPLVSPSSP +IQNWT
Sbjct: 1   MADSHRRTSPTTTRHPQQPSRLQRRAPASLQINRSDWKVAIPLLSPLVSPSSPKDIQNWT 60

Query: 61  ADS------GSISSSSSHHHHHH-----HHHHKESEPEKPPVFKKWQHPAAPFHYDSGSR 117
            DS       S SSSSSHHHHHH     H HHKE E EK PVFKKWQHPAAPFHYDSG R
Sbjct: 61  PDSREASSASSSSSSSSHHHHHHHQHHQHQHHKEPEAEKAPVFKKWQHPAAPFHYDSGPR 120

BLAST of Carg10953 vs. ExPASy TrEMBL
Match: A0A0A0LLJ8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G190740 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.4e-33
Identity = 92/123 (74.80%), Postives = 100/123 (81.30%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSP-SSPTEIQNW- 60
           MA+S   P+  AA    PSRLQRRAPASLQI+RSDWKVAIPLLSPLVSP SSP +I +W 
Sbjct: 1   MADS-HPPTSPAA---TPSRLQRRAPASLQINRSDWKVAIPLLSPLVSPSSSPKDIHSWS 60

Query: 61  TADS---GSISSSSSHHHH--HHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAF 117
           T DS    S +SSSSHHHH  HHHHHHKES+PEK PVFKKWQHPAAPFHYDSG+RAPR F
Sbjct: 61  TPDSRQPSSTTSSSSHHHHLLHHHHHHKESDPEKAPVFKKWQHPAAPFHYDSGARAPR-F 118

BLAST of Carg10953 vs. ExPASy TrEMBL
Match: A0A6J1CA35 (uncharacterized protein At4g14450, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111009774 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.0e-28
Identity = 84/119 (70.59%), Postives = 91/119 (76.47%), Query Frame = 0

Query: 1   MAESQRRPSGTAARK-QQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQNWT 60
           MA++QRR SG  +R+ QQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQN  
Sbjct: 1   MADTQRRTSGAGSRRQQQPSRLQRRAPASLQISRSDWKVAIPLLSPLVSPSSPTEIQN-- 60

Query: 61  ADSGSISSSSSHHHHHHHHHHKESEPEKP--PVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
             S   S+SS           + S+PEKP  PV KKWQHPAAPFHYDSG RAPR FVPV
Sbjct: 61  --SREPSTSS-----------QGSDPEKPPVPVTKKWQHPAAPFHYDSGPRAPR-FVPV 103

BLAST of Carg10953 vs. TAIR 10
Match: AT1G04330.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G23170.1); Has 74 Blast hits to 74 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 63.2 bits (152), Expect = 1.6e-10
Identity = 48/116 (41.38%), Postives = 59/116 (50.86%), Query Frame = 0

Query: 1   MAESQRRPSGTAARKQQPSRLQRRAPASLQIS--RSDWKVAIPLLSPLVS-PSSPTEIQN 60
           M+ + R  SG+  RK   SRLQRRAP  L+I+   ++WKVAIPLLSP  S P  P  +  
Sbjct: 1   MSSTARNISGSGNRKS--SRLQRRAPPPLKINPCEANWKVAIPLLSPTESPPQKPPAVMK 60

Query: 61  WTADSGSISSSSSHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAF 114
                                     E EKPPVFKKWQHPAAPF+Y     + + F
Sbjct: 61  ------------------REEQRWGKEAEKPPVFKKWQHPAAPFYYQPAPSSNQPF 96

BLAST of Carg10953 vs. TAIR 10
Match: AT3G23170.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G14450.1); Has 74 Blast hits to 74 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 58.5 bits (140), Expect = 4.0e-09
Identity = 44/107 (41.12%), Postives = 57/107 (53.27%), Query Frame = 0

Query: 15  KQQPSRLQRRAPASLQI----SRSDWKVAIPLLSPL-VSPSSPTEIQNWTADSGSISSSS 74
           ++QPSRL +R PA   +    + ++W  AIPLLSPL +SP S         D   +  + 
Sbjct: 18  RRQPSRLLKRPPALKIVPATPAANNWNTAIPLLSPLALSPESS------PVDQPPVEKNQ 77

Query: 75  SHHHHHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVPV 117
           S               EK PVFKKWQHPAAPF+Y+S +  P  FVPV
Sbjct: 78  S----------TAVAVEKTPVFKKWQHPAAPFYYESSTFVP-PFVPV 107

BLAST of Carg10953 vs. TAIR 10
Match: AT4G14450.1 (unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G23170.1); Has 74 Blast hits to 74 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.8 bits (125), Expect = 2.2e-07
Identity = 48/117 (41.03%), Postives = 62/117 (52.99%), Query Frame = 0

Query: 5   QRRPSGTAARKQQPSRLQRRAPASL--QISRSDWKVAIPLLSPLVSPSSPTEIQNWTADS 64
           QR  S  A+ ++Q S+LQRRAP+ +    S S+W VAIPLLSPL               +
Sbjct: 24  QRTSSSAASNERQLSQLQRRAPSLMIKPTSFSNWNVAIPLLSPL---------------A 83

Query: 65  GSISSSSSHHH----HHHHHHHKESEPEKPPVFKKWQHPAAPFHYDSGSRAPRAFVP 116
            S++SS    H     +      E E +K PVFKKWQHPA+PF Y+     P  FVP
Sbjct: 84  PSLTSSFDQSHVPPPQNKTEIPVEEEVKKTPVFKKWQHPASPFCYE-----PTTFVP 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6593848.13.1e-56100.00putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]... [more]
XP_023000559.11.2e-5297.41uncharacterized protein At4g14450, chloroplastic-like [Cucurbita maxima][more]
XP_022930471.11.6e-5297.41uncharacterized protein At4g14450, chloroplastic-like [Cucurbita moschata][more]
XP_023515028.15.2e-5193.97uncharacterized protein At4g14450, chloroplastic-like [Cucurbita pepo subsp. pep... [more]
XP_038875425.13.0e-3880.34uncharacterized protein At4g14450, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q6NN023.1e-0641.03Uncharacterized protein At4g14450, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1KKB86.0e-5397.41uncharacterized protein At4g14450, chloroplastic-like OS=Cucurbita maxima OX=366... [more]
A0A6J1ER117.8e-5397.41uncharacterized protein At4g14450, chloroplastic-like OS=Cucurbita moschata OX=3... [more]
A0A1S3CDB19.3e-3875.78uncharacterized protein At4g14450, chloroplastic-like OS=Cucumis melo OX=3656 GN... [more]
A0A0A0LLJ82.4e-3374.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G190740 PE=4 SV=1[more]
A0A6J1CA353.0e-2870.59uncharacterized protein At4g14450, chloroplastic-like OS=Momordica charantia OX=... [more]
Match NameE-valueIdentityDescription
AT1G04330.11.6e-1041.38unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G23170.14.0e-0941.12unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G14450.12.2e-0741.03unknown protein; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein matc... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..116
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR33912:SF2PUTATIVE-RELATEDcoord: 1..114
IPR040381Uncharacterized protein At4g14450-likePANTHERPTHR33912OS01G0939400 PROTEINcoord: 1..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10953-RACarg10953-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus