Carg10819 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10819
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionvacuolar protein sorting-associated protein 32 homolog 2-like
LocationCarg_Chr05: 11939710 .. 11943184 (+)
RNA-Seq ExpressionCarg10819
SyntenyCarg10819
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGGAAACCAGTGAGTAGATTGCCGCGTGGAGACGAAACAGCGACGTGAAGAGAGCAGGAACACTAAATCTTCGTCCTCCGGAGTCTGGAGCGATTGAGGAAGCCTTTCTACTTCAAATTTCTTCACAAATTCTTTCGGTTCATTTCTGCTTCTCATATTTCCGTCTCAATCTCCCTGGTTTATCGACTTTTGGTAGGAATTGATTGTTTTTGTTTTCATCGAAGTTGATTGGCTGTTTGAAACTAAGCATTTGGAACTAAGCGTTTGGTTCTGATTTTGTTCTTTGTTGCAGAAGATCGAGAGAGGATTCTTGTTTCTTAGCGTCAACAATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGAGAAGCTAAGCGAGGTACGTCTTCACTTTGTTGTTCTATTGTTTTAGATATTCTTTTTATGCAAACGATCTTTTCCTTTAGTAGATTGTTCTGAACTCTAGCTCTATTTCTTTCAATCTTGATTGATATTTGACTTCTCGATCATTCACCTCTTCCAAATATTATTGTTTATGACGACTTAGTAATTGGTCCTCCATGACGGAGTATTAGTGTTTATGACGCCGCGATAATTAGTTCTCACTTCTCCGTGACCGAGTATTAGTGTTTATTTGGTTGTTCATTTTACTTTGTCAAGTGGTGGTTCCGGTTGAGAAATCATCGTCTTATTTGAATTAGGTTAACATGTAATTTTACACTTGTTCTGCTACTTTTAATCGCGGTACACTAGGTGAGATTTCACAGATGTAAGAACTGAAAACTGTGTACTACATTTCTATGCTATTCGTCTATGCGATTATATTGGAGTGGATAGTTGAAATCATGTCCAAAGTGATGGTAATCGTTATCGCACAGACCTTTTTAGTCTAACAAAGCTTTTCATAATTGGTAGCAATGTTTTTGGGCACTTTTTTTTCACAGTCTGTAATGATCTGTTTAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTGTAAAGAAGGCATCTGCAGAGGTTGAAAAAGCTAAAGAGTTCACAAGAGTGAAGAATAAACGAGGTATGGCCTGAAATTGAGTGATCTCATCTATGACGATATTGATTTCAATTTCCTTTGATTTTTTAAATGTGTGGAGCGTGATTAAGATTTAATTAATACCTACGTTGTCGACTCTGAGGTGTAAATTTGATGTCGCACCATACCTGGTTTTCTCACGTTTCGAAGTTTTTTAATCCGTAAAAACTTTTGAAATGCAAGTTATGCTTATATCAGTTTACTGCAATGTAATAATCTGCACGAACTTTTAATGCTGTTTTTCAAGTACTTTTTTTTGTTCATTTTAGTTATTTTTTATTATTAGAAGGAGAGTCTTGTTCTCAATGAATTCTACGAAGCAGCTTCTCGCTTGTTAGGGAAATAAATCTCATGAACGGTTTTTTGTGGGTTAGAGAATACACACTGCATTTTTTTTTTTTCAAATAAGTAGAAGGCATTGTGATTTGAAAATGTTGATATTACTCTTTTCATGTATATAATCAATTTTGATTACATGACAATTGTCCCGTGGTAGTTTGAATACAGCTATTCTTGAGTTAATAGAGCCATAAGGACTTGATGTTTTTCAGATTCCATTCACAATCATTATATTTTGTTTGCAGCGGCCATACAATGTTTGAAGAGAAAGAGACTCTACGAACAGCAAGTAGAGCAGCTTGGAAATTTTCAATTGCGTATCCACGATCAGGTGTGCTGTAGTCGTTATTTACTGATTGATGAGACGTCTATTTCTTTTACTTTCATGAGCTGCAATGAAGCTTTTACTCAAAACCTATTCTTACTCTTGCAGATGATTATGTTAGAAGGCGCTAAAGCTACGACGGAAACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCAATGCAGAAAGCAACGTATGCCTACTTTTATTTGCCTTTGTGGTTTATAATCTTTCAGAATTTTGGTCTTGCTGGGTCATTCAATATCTATAAAAAAATAAATCAGTCTATGTTATATAGTACACCTTAGCACGTCTTTTAACCATATTATTATTATTGTCTGAAAATTGTACAATATATCTGAACTTATTTCATCACTTCATTCATCTAGTTAAGCTAGCAGCCTGGAGAAAATGCGATCAAATTTTGTTCATTTAAATATATTTTAACGGCCTGAAATTTGAATGGTTTGTGAAATTTTTCTTCATGTTTTAGTGAGAAGTTAGAGCTGTAGTTTATATAGTAAGTAGCTTAATTATTCGGAACTTCTTCAGAAATATTGATGATGTGGACAAAACGATGGATGAGATTAATGAGCAGACGGAGAATATGAAACAGATACAGGAAGCGCTATCAACTCCAATTGGTGCTGCAGCTGACTTCGACGAGGTTGTTAATCTTTTACTCTTTCCTATTATTAATAGCTAGCTTATATCTAGTAAGTATCTCATCTAGTGATTATGGTTGCCATTCAGGATGAATTGGAGGCGGAACTTGAAGAGCTGGAAAGTGCGGAGTTGGAAGAGCAACTTCTCCAACCGGCGTCAACAACTCCTGCTGCGCCCGTTTCCATCCCGTCAGGCCGAGTACCTGCACGCCCTGTTCCTCAGAAGAGAACTGCTGAAGAAGATGAACTCGCAGCTCTGCAAGCGGAGATGGCCCTCTAATCAGGTCTTGTTCTTTCAACGTTTTATATTTTATTTGAAAGATGTCAATGGAGTTTATGCACTACTGTTATCTTGCAATTGAATCGGTGTGATCTCCTGGCAGATTGTGCCATACAAAGAAGCTTACGATGGGCGTTCGTTCAAAATTGCATACGAGCTATTCTTCCCTGCCGGGTGAAGTTGTATATAAAGAACGCGAAATAACTGTAATCATCCTTTAAATCTTTTATTTATCTATACTCTATTTTGCAGGGAATGCAAACATTGTGGTCATTTAAAATTTAATTTCGTGCGCCTAGGGAAATTTAGGTACAAACTTCAGAGTAATTTTATTCATAGTTTCATAATTTTATTCATATTTTCTTGGTTTATAATAGTAATCGCATATTGGTTTAGGAAGACTACTACTAATAAATATATGATCTAAAATTAAGTTTGAGTTTTTGTTCCTGATTGATATTCAGGATTTTGAAGTGTAAAAAATTTTGAACTTTAATGTTCACAATCAATTCATCAGTCATAAATTTAAATTCGGCCTAATAAAGCTTCAATTCAAATTTTACAAATATAAATACTAGATGGAATAATTCACAAATAGTACTGCAACTCAAAACAGAAGATATTTAACATACGAAAAAGTAACAGCTTTTTTTAGTTTATAGTGAGCTTCGAAAATGAAAAGAAATCATACAAATTTCACATTTGAAAATTCGTTTAAACAATTTGTAAGTCTCAATACAATTTCTAGTAAATGTTTA

mRNA sequence

ACGGAAACCAGTGAGTAGATTGCCGCGTGGAGACGAAACAGCGACGTGAAGAGAGCAGGAACACTAAATCTTCGTCCTCCGGAGTCTGGAGCGATTGAGGAAGCCTTTCTACTTCAAATTTCTTCACAAATTCTTTCGGTTCATTTCTGCTTCTCATATTTCCGTCTCAATCTCCCTGGTTTATCGACTTTTGAAGATCGAGAGAGGATTCTTGTTTCTTAGCGTCAACAATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGAGAAGCTAAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTGTAAAGAAGGCATCTGCAGAGGTTGAAAAAGCTAAAGAGTTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTTTGAAGAGAAAGAGACTCTACGAACAGCAAGTAGAGCAGCTTGGAAATTTTCAATTGCGTATCCACGATCAGATGATTATGTTAGAAGGCGCTAAAGCTACGACGGAAACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCAATGCAGAAAGCAACAAATATTGATGATGTGGACAAAACGATGGATGAGATTAATGAGCAGACGGAGAATATGAAACAGATACAGGAAGCGCTATCAACTCCAATTGGTGCTGCAGCTGACTTCGACGAGGATGAATTGGAGGCGGAACTTGAAGAGCTGGAAAGTGCGGAGTTGGAAGAGCAACTTCTCCAACCGGCGTCAACAACTCCTGCTGCGCCCGTTTCCATCCCGTCAGGCCGAGTACCTGCACGCCCTGTTCCTCAGAAGAGAACTGCTGAAGAAGATGAACTCGCAGCTCTGCAAGCGGAGATGGCCCTCTAATCAGATTGTGCCATACAAAGAAGCTTACGATGGGCGTTCGTTCAAAATTGCATACGAGCTATTCTTCCCTGCCGGGTGAAGTTGTATATAAAGAACGCGAAATAACTGTAATCATCCTTTAAATCTTTTATTTATCTATACTCTATTTTGCAGGGAATGCAAACATTGTGGTCATTTAAAATTTAATTTCGTGCGCCTAGGGAAATTTAGGTACAAACTTCAGAGTAATTTTATTCATAGTTTCATAATTTTATTCATATTTTCTTGGTTTATAATAGTAATCGCATATTGGTTTAGGAAGACTACTACTAATAAATATATGATCTAAAATTAAGTTTGAGTTTTTGTTCCTGATTGATATTCAGGATTTTGAAGTGTAAAAAATTTTGAACTTTAATGTTCACAATCAATTCATCAGTCATAAATTTAAATTCGGCCTAATAAAGCTTCAATTCAAATTTTACAAATATAAATACTAGATGGAATAATTCACAAATAGTACTGCAACTCAAAACAGAAGATATTTAACATACGAAAAAGTAACAGCTTTTTTTAGTTTATAGTGAGCTTCGAAAATGAAAAGAAATCATACAAATTTCACATTTGAAAATTCGTTTAAACAATTTGTAAGTCTCAATACAATTTCTAGTAAATGTTTA

Coding sequence (CDS)

ATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGAGAAGCTAAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTGTAAAGAAGGCATCTGCAGAGGTTGAAAAAGCTAAAGAGTTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTTTGAAGAGAAAGAGACTCTACGAACAGCAAGTAGAGCAGCTTGGAAATTTTCAATTGCGTATCCACGATCAGATGATTATGTTAGAAGGCGCTAAAGCTACGACGGAAACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCAATGCAGAAAGCAACAAATATTGATGATGTGGACAAAACGATGGATGAGATTAATGAGCAGACGGAGAATATGAAACAGATACAGGAAGCGCTATCAACTCCAATTGGTGCTGCAGCTGACTTCGACGAGGATGAATTGGAGGCGGAACTTGAAGAGCTGGAAAGTGCGGAGTTGGAAGAGCAACTTCTCCAACCGGCGTCAACAACTCCTGCTGCGCCCGTTTCCATCCCGTCAGGCCGAGTACCTGCACGCCCTGTTCCTCAGAAGAGAACTGCTGAAGAAGATGAACTCGCAGCTCTGCAAGCGGAGATGGCCCTCTAA

Protein sequence

MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTTPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL
Homology
BLAST of Carg10819 vs. NCBI nr
Match: KAG7030473.1 (Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.1 bits (1006), Expect = 3.1e-105
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Carg10819 vs. NCBI nr
Match: XP_023545615.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545619.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545620.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547008.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547009.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547010.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547011.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.8 bits (1000), Expect = 1.5e-104
Identity = 216/218 (99.08%), Postives = 217/218 (99.54%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           TPAAPVS+PSGRVPARPVPQKR AEEDELAALQAEMAL
Sbjct: 181 TPAAPVSVPSGRVPARPVPQKRNAEEDELAALQAEMAL 218

BLAST of Carg10819 vs. NCBI nr
Match: XP_022946105.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946106.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946107.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946108.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata])

HSP 1 Score: 389.4 bits (999), Expect = 2.0e-104
Identity = 216/218 (99.08%), Postives = 217/218 (99.54%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           TPAAPVSIPSGRVPARPVPQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of Carg10819 vs. NCBI nr
Match: XP_022999526.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022999527.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022999528.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022999529.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima])

HSP 1 Score: 383.6 bits (984), Expect = 1.1e-102
Identity = 213/218 (97.71%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAELEEQLLQ AST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELEEQLLQQAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           TPAAPVS+PSGRVPARPVP+KRTAEE+ELAALQAEMAL
Sbjct: 181 TPAAPVSVPSGRVPARPVPRKRTAEEEELAALQAEMAL 218

BLAST of Carg10819 vs. NCBI nr
Match: XP_022937864.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937865.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937866.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937867.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_023536810.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536811.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536812.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >KAG7021320.1 Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.1 bits (980), Expect = 3.2e-102
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PA PVS+PSGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Carg10819 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 1.7e-93
Identity = 191/219 (87.21%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 TPAAP-VSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            P  P V +P+GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Carg10819 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 4.8e-85
Identity = 182/217 (83.87%), Postives = 192/217 (88.48%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAIQC 62
           NRLFGKPKQE + L TL+KL+ETLEMLEKKENVL+KKA+ EVEKAKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTTP 182
           DKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSIPSGRVPARPVPQKR-TAEEDELAALQAEMAL 219
             P+ +P G  PAR   QK+ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Carg10819 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.1e-28
Identity = 88/217 (40.55%), Postives = 127/217 (58.53%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKR 60
           +F ++F   GK  + A+    +++L ET EML KK+  L KK   E+  AK+    KNKR
Sbjct: 3   LFGKMFGSGGKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQP 180
           +ID VD+ M +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LL+ 
Sbjct: 123 DIDKVDELMQDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLLEI 182

Query: 181 ASTTPAAPVSIPSGRVPARPVPQKRTAEEDELAALQA 215
               P    ++PS  +P+RP  +K   +ED++  L+A
Sbjct: 183 GDNVPLP--NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

BLAST of Carg10819 vs. ExPASy Swiss-Prot
Match: Q6IQ73 (Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.7e-26
Identity = 91/217 (41.94%), Postives = 123/217 (56.68%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKR 60
           +F +LF   GK  +       ++KL ET EML KK++ L KK  AE+  AK+    KNKR
Sbjct: 3   VFGKLFGGGGKGGKGPTPQEAIQKLRETEEMLAKKQDFLEKKIDAELLIAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMK   +  
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKTAHENM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQP 180
           +ID VD  M +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LLQ 
Sbjct: 123 DIDKVDDLMQDITEQQELAQEISDAISRPVGFGEEFDEDELMAELEELEQEELDKDLLQ- 182

Query: 181 ASTTPAAPVSIPSGRVPARPVP------QKRTAEEDE 209
                + P  +P   VP+ P+P      QK+  EEDE
Sbjct: 183 ----ISGPEDVPLPNVPSNPLPKKTAVAQKKRQEEDE 213

BLAST of Carg10819 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.1e-26
Identity = 81/207 (39.13%), Postives = 121/207 (58.45%), Query Frame = 0

Query: 7   GKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAIQCLKRK 66
           GK  +  +    ++KL +T EML KK+  L KK   E+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKNKRAALQALKRK 71

Query: 67  RLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     +I+ VD+ M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNMDIEKVDELM 131

Query: 127 DEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTTPAAPV 186
            +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL+          
Sbjct: 132 QDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLLEVQGPETVPLP 191

Query: 187 SIPSGRVPARPVPQKRTAEEDELAALQ 214
           ++P+  +PA+PV +K+  ++D++  L+
Sbjct: 192 NVPAASLPAKPVKKKQEEDDDDMRELE 217

BLAST of Carg10819 vs. ExPASy TrEMBL
Match: A0A6J1G2S3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450291 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 9.7e-105
Identity = 216/218 (99.08%), Postives = 217/218 (99.54%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           TPAAPVSIPSGRVPARPVPQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of Carg10819 vs. ExPASy TrEMBL
Match: A0A6J1KB41 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493861 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.3e-103
Identity = 213/218 (97.71%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAELEEQLLQ AST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELEEQLLQQAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           TPAAPVS+PSGRVPARPVP+KRTAEE+ELAALQAEMAL
Sbjct: 181 TPAAPVSVPSGRVPARPVPRKRTAEEEELAALQAEMAL 218

BLAST of Carg10819 vs. ExPASy TrEMBL
Match: A0A6J1FBK1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444128 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PA PVS+PSGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Carg10819 vs. ExPASy TrEMBL
Match: A0A1S3CAQ7 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103498351 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.6e-102
Identity = 209/218 (95.87%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P+GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Carg10819 vs. ExPASy TrEMBL
Match: A0A5A7V4V7 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00800 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.6e-102
Identity = 209/218 (95.87%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKA+AEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PAAPVS+P+GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Carg10819 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.2e-94
Identity = 191/219 (87.21%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 TPAAP-VSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            P  P V +P+GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Carg10819 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.2e-94
Identity = 191/219 (87.21%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TL+KL+ETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 TPAAP-VSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            P  P V +P+GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Carg10819 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 315.5 bits (807), Expect = 3.4e-86
Identity = 182/217 (83.87%), Postives = 192/217 (88.48%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAIQC 62
           NRLFGKPKQE + L TL+KL+ETLEMLEKKENVL+KKA+ EVEKAKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTTP 182
           DKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSIPSGRVPARPVPQKR-TAEEDELAALQAEMAL 219
             P+ +P G  PAR   QK+ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Carg10819 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 300.1 bits (767), Expect = 1.5e-81
Identity = 168/192 (87.50%), Postives = 179/192 (93.23%), Query Frame = 0

Query: 28  MLEKKENVLVKKASAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQM 87
           MLEKKE VL+KKA AEVEKAKE++R KNKRAAIQCLKRKRLYE QVEQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  IMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPI 147
           IMLEGAKATTETVDALR+GASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+TP+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GAAADFDEDELEAELEELESAELEEQLLQPASTTPAAP-VSIPSGRVPARPVPQKRTAEE 207
           GAAADFDEDEL AEL+ELES ELE QLLQPA+T P  P V +P+GR PARPVPQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 208 DELAALQAEMAL 219
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

BLAST of Carg10819 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 56.2 bits (134), Expect = 3.7e-08
Identity = 62/207 (29.95%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60
           M N    KPK      A L  L      L + +  L K   AE + A++  R K K  A+
Sbjct: 1   MGNLFVKKPKITEVDRAIL-SLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRAL 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
             LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G +A+KA+Q   N+D
Sbjct: 61  LALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLD 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADF-DEDELEAELEELESAELEEQLLQPAS 180
           DV K MD+    T   K  Q+ LS  +G      DE+E+ AE + LES  + E + +  +
Sbjct: 121 DVQKLMDD----TAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPT 180

Query: 181 T--TPAAPVSIPSGRVPAR-PVPQKRT 204
           T   P  P  +    VP + PV    T
Sbjct: 181 TELMPEEPEKMDLPDVPTKAPVASNET 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030473.13.1e-105100.00Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma su... [more]
XP_023545615.11.5e-10499.08vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo su... [more]
XP_022946105.12.0e-10499.08vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
XP_022999526.11.1e-10297.71vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima]... [more]
XP_022937864.13.2e-10296.33vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >X... [more]
Match NameE-valueIdentityDescription
Q9SZE41.7e-9387.21Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821974.8e-8583.87Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC47.1e-2840.55Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q6IQ731.7e-2641.94Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=... [more]
Q5XGW65.1e-2639.13Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1G2S39.7e-10599.08vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A6J1KB415.3e-10397.71vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxim... [more]
A0A6J1FBK11.6e-10296.33vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata O... [more]
A0A1S3CAQ72.6e-10295.87vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A5A7V4V72.6e-10295.87Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
Match NameE-valueIdentityDescription
AT4G29160.11.2e-9487.21SNF7 family protein [more]
AT4G29160.31.2e-9487.21SNF7 family protein [more]
AT2G19830.13.4e-8683.87SNF7 family protein [more]
AT4G29160.21.5e-8187.50SNF7 family protein [more]
AT5G09260.13.7e-0829.95vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..175
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 12..39
NoneNo IPR availableGENE3D6.10.250.1710coord: 117..172
e-value: 5.1E-9
score: 37.9
NoneNo IPR availableGENE3D1.10.287.1060coord: 14..91
e-value: 2.6E-22
score: 80.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..207
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..218
NoneNo IPR availablePANTHERPTHR22761:SF71SNF7 FAMILY-RELATEDcoord: 1..218
IPR005024Snf7 familyPFAMPF03357Snf7coord: 20..185
e-value: 2.8E-45
score: 154.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10819-RACarg10819-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body