Carg10803 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10803
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionheat stress transcription factor A-2-like
LocationCarg_Chr05: 12010850 .. 12012482 (+)
RNA-Seq ExpressionCarg10803
SyntenyCarg10803
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTAACCAAACAACCCTCTCTGGCGGTGGCGGTGGCGGTGGTGGCTGCCCCGACGGCGCTGCAACTGCAAGCGGTGACATCATGGATCAAAGGACTGTTAAGGAAGAGGAGATGGAAGTAAGCACGGAGACGATGGAGGGCGGTGGAGGCGAATCTGGCGGAGGCGATGGGGGTTGGGCGTCATCGGCGACGGCGAAACCAATGGAAGGGTTGCATGAGGTGGGGCCGGCGCCGTTCCTGAAGAAGACGTATGAGATGGTGGAGGATCCGGAAACCGACCCGGTTGTATCTTGGAGCGAAGGCCGTAATAGCTTCATTGTTTGGGATTCTCATAAACTCTCCATCACTCTTCTCCCCAAATACTTCAAGCACTCCAATTTCTCCAGCTTCATCCGCCAGCTCAACACTTATGTCAGTATAAACCAATTTAATTTATTTCCTATTTTCCTTACTTTTTCTTTTTTATTTTTAATATCTCAATCCTAAAATATATATCTATATATCTTTTTTTTTTTATTCAGAAATATTATTTTATAAATTACTCTCTCTCTCTGAACCAATTCCAAACAACTACTACTTGGTATGCTTATAGTGACAAAAGTGAAGACTGGATTTCAATTTTATTGTCTTCTGTTCATTTAGGTCTCGGTTGATAACCATTTTGTTACGTTCATATCATATAATCAACCATTTTGTTACGTTCATATCATATATACATACGGTGTTGAGTTGAATTTTGAAAATATTTTAAGAACGTGGATAACCCATGAAAGAAATTCATGGGTTCCCGGGCAAGTAGGTGGAAGTAGTAATTTATAAGCTTAATTCATGGTTAACCCATGAAAGAAATTTCATGGATTTACGAGCAAGGCAGGTGGGAGATGGGAGAGTAGCATTTATAAGCTTAATTTGGGTATTGTTGCAGGGTTTTAGAAAGATTGATAGGGATAAATGGGAGTTTGCAAACGAAGGGTTTCAAGGAGGGAAGAAGCATTTACTAAAGAACATAAAAAGAAGAAGCAGGTACAATAATTACAAGAAGCAGCAACAACATTTGGGCTTACAAGATTTGTCAAACCACTCACTTGTTGAAGCAGAACTACAAGCTTTGAAGTCCGACAACAACATTTTGAGAGTAGAGATGTTGAAACTGAGAGACCAGCAGCAGGACTCACACAACCACCTCACCATGGTTGAAGACCGAGTCCGATGCGTCGAGTCCAAGTACCAACAGATGCTCTCTTTCGTCTCTAAAATGTCGAGAGATCCCGGGTTCTGTCGACAGTTGGTGCAGAGGAGAATGCTGAGGAAGAAGCTGATCAACAATGGGGATGAGTTTGGAAACAACAGAAGCTTACTTGCAATGCAAGCCCATCAAAATCTCACCCACTCTGAACTCATCACTACTGAAATGATTCCACAAGCTATCGAACCCGGACCGGTCGTGCTCGAGCCGCCGGTCCGACCCTCCACAATGGTGGTTGATGAAGACAGTGACTCCAAATTCTTTCTGGAATTGGAGGATCTCATCCAGAAGCCTCCTCCTCCTCCTCCTCCTCCTCCTCAGGATTGCCCCTCAGGTTACGGACAGCAACAAGCTTTCCATGGATTTGTTGGATCCATTCCATGA

mRNA sequence

ATGATTAACCAAACAACCCTCTCTGGCGGTGGCGGTGGCGGTGGTGGCTGCCCCGACGGCGCTGCAACTGCAAGCGGTGACATCATGGATCAAAGGACTGTTAAGGAAGAGGAGATGGAAGTAAGCACGGAGACGATGGAGGGCGGTGGAGGCGAATCTGGCGGAGGCGATGGGGGTTGGGCGTCATCGGCGACGGCGAAACCAATGGAAGGGTTGCATGAGGTGGGGCCGGCGCCGTTCCTGAAGAAGACGTATGAGATGGTGGAGGATCCGGAAACCGACCCGGTTGTATCTTGGAGCGAAGGCCGTAATAGCTTCATTGTTTGGGATTCTCATAAACTCTCCATCACTCTTCTCCCCAAATACTTCAAGCACTCCAATTTCTCCAGCTTCATCCGCCAGCTCAACACTTATGGTTTTAGAAAGATTGATAGGGATAAATGGGAGTTTGCAAACGAAGGGTTTCAAGGAGGGAAGAAGCATTTACTAAAGAACATAAAAAGAAGAAGCAGGTACAATAATTACAAGAAGCAGCAACAACATTTGGGCTTACAAGATTTGTCAAACCACTCACTTGTTGAAGCAGAACTACAAGCTTTGAAGTCCGACAACAACATTTTGAGAGTAGAGATGTTGAAACTGAGAGACCAGCAGCAGGACTCACACAACCACCTCACCATGGTTGAAGACCGAGTCCGATGCGTCGAGTCCAAGTACCAACAGATGCTCTCTTTCGTCTCTAAAATGTCGAGAGATCCCGGGTTCTGTCGACAGTTGGTGCAGAGGAGAATGCTGAGGAAGAAGCTGATCAACAATGGGGATGAGTTTGGAAACAACAGAAGCTTACTTGCAATGCAAGCCCATCAAAATCTCACCCACTCTGAACTCATCACTACTGAAATGATTCCACAAGCTATCGAACCCGGACCGGTCGTGCTCGAGCCGCCGGTCCGACCCTCCACAATGGTGGTTGATGAAGACAGTGACTCCAAATTCTTTCTGGAATTGGAGGATCTCATCCAGAAGCCTCCTCCTCCTCCTCCTCCTCCTCCTCAGGATTGCCCCTCAGGTTACGGACAGCAACAAGCTTTCCATGGATTTGTTGGATCCATTCCATGA

Coding sequence (CDS)

ATGATTAACCAAACAACCCTCTCTGGCGGTGGCGGTGGCGGTGGTGGCTGCCCCGACGGCGCTGCAACTGCAAGCGGTGACATCATGGATCAAAGGACTGTTAAGGAAGAGGAGATGGAAGTAAGCACGGAGACGATGGAGGGCGGTGGAGGCGAATCTGGCGGAGGCGATGGGGGTTGGGCGTCATCGGCGACGGCGAAACCAATGGAAGGGTTGCATGAGGTGGGGCCGGCGCCGTTCCTGAAGAAGACGTATGAGATGGTGGAGGATCCGGAAACCGACCCGGTTGTATCTTGGAGCGAAGGCCGTAATAGCTTCATTGTTTGGGATTCTCATAAACTCTCCATCACTCTTCTCCCCAAATACTTCAAGCACTCCAATTTCTCCAGCTTCATCCGCCAGCTCAACACTTATGGTTTTAGAAAGATTGATAGGGATAAATGGGAGTTTGCAAACGAAGGGTTTCAAGGAGGGAAGAAGCATTTACTAAAGAACATAAAAAGAAGAAGCAGGTACAATAATTACAAGAAGCAGCAACAACATTTGGGCTTACAAGATTTGTCAAACCACTCACTTGTTGAAGCAGAACTACAAGCTTTGAAGTCCGACAACAACATTTTGAGAGTAGAGATGTTGAAACTGAGAGACCAGCAGCAGGACTCACACAACCACCTCACCATGGTTGAAGACCGAGTCCGATGCGTCGAGTCCAAGTACCAACAGATGCTCTCTTTCGTCTCTAAAATGTCGAGAGATCCCGGGTTCTGTCGACAGTTGGTGCAGAGGAGAATGCTGAGGAAGAAGCTGATCAACAATGGGGATGAGTTTGGAAACAACAGAAGCTTACTTGCAATGCAAGCCCATCAAAATCTCACCCACTCTGAACTCATCACTACTGAAATGATTCCACAAGCTATCGAACCCGGACCGGTCGTGCTCGAGCCGCCGGTCCGACCCTCCACAATGGTGGTTGATGAAGACAGTGACTCCAAATTCTTTCTGGAATTGGAGGATCTCATCCAGAAGCCTCCTCCTCCTCCTCCTCCTCCTCCTCAGGATTGCCCCTCAGGTTACGGACAGCAACAAGCTTTCCATGGATTTGTTGGATCCATTCCATGA

Protein sequence

MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGWASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTEMIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCPSGYGQQQAFHGFVGSIP
Homology
BLAST of Carg10803 vs. NCBI nr
Match: KAG7030489.1 (Heat shock factor protein HSF30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 740.3 bits (1910), Expect = 7.9e-210
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0

Query: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60
           MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW
Sbjct: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60

Query: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120
           ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP
Sbjct: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120

Query: 121 KYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180
           KYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ
Sbjct: 121 KYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180

Query: 181 HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ 240
           HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ
Sbjct: 181 HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ 240

Query: 241 QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE 300
           QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE
Sbjct: 241 QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE 300

Query: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCPSGYGQ 360
           MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCPSGYGQ
Sbjct: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCPSGYGQ 360

Query: 361 QQAFHGFVGSIP 373
           QQAFHGFVGSIP
Sbjct: 361 QQAFHGFVGSIP 372

BLAST of Carg10803 vs. NCBI nr
Match: XP_022946498.1 (heat stress transcription factor A-2-like [Cucurbita moschata])

HSP 1 Score: 731.1 bits (1886), Expect = 4.8e-207
Identity = 370/372 (99.46%), Postives = 370/372 (99.46%), Query Frame = 0

Query: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60
           MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW
Sbjct: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60

Query: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120
           ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP
Sbjct: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120

Query: 121 KYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180
           KYFKHSNFSSFIRQLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ
Sbjct: 121 KYFKHSNFSSFIRQLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180

Query: 181 HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ 240
           HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ
Sbjct: 181 HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ 240

Query: 241 QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE 300
           QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE
Sbjct: 241 QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE 300

Query: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCPSGYGQ 360
           MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQK PPPPPPPPQDCPSGYGQ
Sbjct: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQK-PPPPPPPPQDCPSGYGQ 360

Query: 361 QQAFHGFVGSIP 373
           QQAFHGFVGSIP
Sbjct: 361 QQAFHGFVGSIP 371

BLAST of Carg10803 vs. NCBI nr
Match: KAG6599513.1 (Heat shock factor protein HSF30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 722.2 bits (1863), Expect = 2.2e-204
Identity = 364/372 (97.85%), Postives = 368/372 (98.92%), Query Frame = 0

Query: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60
           MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW
Sbjct: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60

Query: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120
           ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP
Sbjct: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120

Query: 121 KYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180
           KYFKHSNFSSFIRQLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ
Sbjct: 121 KYFKHSNFSSFIRQLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180

Query: 181 HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ 240
           HLGLQDLSNHSLVE+ELQALKSDNNILRVEMLKLR+QQQDSHNHLTMVEDRVRCVESK+Q
Sbjct: 181 HLGLQDLSNHSLVESELQALKSDNNILRVEMLKLREQQQDSHNHLTMVEDRVRCVESKHQ 240

Query: 241 QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE 300
           QMLSF+SKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQ HQNLTHSELITTE
Sbjct: 241 QMLSFISKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQGHQNLTHSELITTE 300

Query: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCPSGYGQ 360
           MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQK  PPPPPPPQDCPSGYGQ
Sbjct: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQK--PPPPPPPQDCPSGYGQ 360

Query: 361 QQAFHGFVGSIP 373
           QQAFHGFVGSIP
Sbjct: 361 QQAFHGFVGSIP 370

BLAST of Carg10803 vs. NCBI nr
Match: XP_023546638.1 (heat stress transcription factor A-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 662.1 bits (1707), Expect = 2.7e-186
Identity = 347/384 (90.36%), Postives = 357/384 (92.97%), Query Frame = 0

Query: 1   MINQTTLS---GGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGES---G 60
           MINQTTLS   GGGGGGGGCPDGAATASGDIMDQR+VKEEEMEVSTETM GGGGES   G
Sbjct: 1   MINQTTLSGGGGGGGGGGGCPDGAATASGDIMDQRSVKEEEMEVSTETMGGGGGESDCGG 60

Query: 61  GGDGGWASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKL 120
           GGDGGWASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEG NSFIVWDSHKL
Sbjct: 61  GGDGGWASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGGNSFIVWDSHKL 120

Query: 121 SITLLPKYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNN 180
           SITLLPKYFKHSNFSSFIRQLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKRRSRYN+
Sbjct: 121 SITLLPKYFKHSNFSSFIRQLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRRSRYNS 180

Query: 181 YKKQQQH------LGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMV 240
           YKKQQQ       +GLQDLS HSLVEAELQALKSDNNILRVEMLKLR+QQQDS N LTMV
Sbjct: 181 YKKQQQQHHLGMPMGLQDLSKHSLVEAELQALKSDNNILRVEMLKLREQQQDSQNQLTMV 240

Query: 241 EDRVRCVESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAH 300
           EDRVRCVESK+QQMLSFVSKMSR+PGFCRQLVQRRMLRKKL+NNGDEFGNNR LLAMQ H
Sbjct: 241 EDRVRCVESKHQQMLSFVSKMSRNPGFCRQLVQRRMLRKKLMNNGDEFGNNRRLLAMQGH 300

Query: 301 QNLTHSELITTEMIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPP 360
           QNLTHSEL++TEM PQAIEPGPVVLEPPVR STM VDEDSDSKFFLELEDLIQK PPPPP
Sbjct: 301 QNLTHSELLSTEMFPQAIEPGPVVLEPPVRASTM-VDEDSDSKFFLELEDLIQK-PPPPP 360

Query: 361 PPPQDCPSGYGQQQAFHGFVGSIP 373
           PPPQDCPS Y +QQAFHGFVGSIP
Sbjct: 361 PPPQDCPSAYVKQQAFHGFVGSIP 382

BLAST of Carg10803 vs. NCBI nr
Match: XP_022999504.1 (heat stress transcription factor A-7a-like [Cucurbita maxima])

HSP 1 Score: 624.0 bits (1608), Expect = 8.3e-175
Identity = 327/377 (86.74%), Postives = 339/377 (89.92%), Query Frame = 0

Query: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGES-GGGDGG 60
           MINQT LS    GGGGCPDGAATASGDIMDQRTVKEEE+EVS  TMEGGGGES  GGDG 
Sbjct: 1   MINQTILS---DGGGGCPDGAATASGDIMDQRTVKEEEIEVSAGTMEGGGGESDSGGDGV 60

Query: 61  WASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLL 120
            ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEG NSFIVWDSHKLSITLL
Sbjct: 61  SASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGGNSFIVWDSHKLSITLL 120

Query: 121 PKYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQ 180
           PKYFKHSNFSSFIRQLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKRRSRYNNYK+QQ
Sbjct: 121 PKYFKHSNFSSFIRQLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKQQQ 180

Query: 181 QHL----GLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCV 240
            HL    GLQDL+ H LVEAEL ALKSDN +LRVEMLKLR+QQ DS N LTMVEDRVRCV
Sbjct: 181 HHLGMPMGLQDLTKHPLVEAELHALKSDNQVLRVEMLKLREQQHDSQNQLTMVEDRVRCV 240

Query: 241 ESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSE 300
           ESK+QQMLSFVSKMSR+PGFCRQLVQRRMLRKKL+NNGDEFGNNR LLAMQ HQNL+HSE
Sbjct: 241 ESKHQQMLSFVSKMSRNPGFCRQLVQRRMLRKKLMNNGDEFGNNRRLLAMQGHQNLSHSE 300

Query: 301 LITTEMIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCP 360
           L++TEM P  IEPGP VLE PVR STMVVDEDSDSKFFLELEDLI+K PPPPP   QDCP
Sbjct: 301 LVSTEMFPHVIEPGPAVLEQPVRASTMVVDEDSDSKFFLELEDLIKK-PPPPPTTTQDCP 360

Query: 361 SGYGQQQAFHGFVGSIP 373
           SGY QQQAFHGFVGSIP
Sbjct: 361 SGYVQQQAFHGFVGSIP 373

BLAST of Carg10803 vs. ExPASy Swiss-Prot
Match: O80982 (Heat stress transcription factor A-2 OS=Arabidopsis thaliana OX=3702 GN=HSFA2 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.9e-58
Identity = 128/268 (47.76%), Postives = 172/268 (64.18%), Query Frame = 0

Query: 34  VKEEEMEVSTETMEGGGGESGGGDGGWASSATAKPMEGLHEVGPAPFLKKTYEMVEDPET 93
           ++E ++E+  ET+   G  +     G  SS++ +PMEGL+E GP PFL KTYEMVEDP T
Sbjct: 1   MEELKVEMEEETVTFTGSVAASSSVG--SSSSPRPMEGLNETGPPPFLTKTYEMVEDPAT 60

Query: 94  DPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANE 153
           D VVSWS GRNSF+VWDSHK S TLLP+YFKHSNFSSFIRQLNTYGFRKID D+WEFANE
Sbjct: 61  DTVVSWSNGRNSFVVWDSHKFSTTLLPRYFKHSNFSSFIRQLNTYGFRKIDPDRWEFANE 120

Query: 154 GFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQ--DLSNHSLVEAELQALKSDNNILRVEM 213
           GF  G+KHLLKNIKRR         QQ  G+   ++  +   + E++ LK D+ +L  E+
Sbjct: 121 GFLAGQKHLLKNIKRRRNMGLQNVNQQGSGMSCVEVGQYGF-DGEVERLKRDHGVLVAEV 180

Query: 214 LKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKLIN 273
           ++LR QQ  S + +  +E R+   E + QQM++F++K   +P F +Q       +K L  
Sbjct: 181 VRLRQQQHSSKSQVAAMEQRLLVTEKRQQQMMTFLAKALNNPNFVQQFAVMSKEKKSLF- 240

Query: 274 NGDEFGNNRSLLAMQA----HQNLTHSE 296
            G + G  R L +  +     +NL H +
Sbjct: 241 -GLDVGRKRRLTSTPSLGTMEENLLHDQ 263

BLAST of Carg10803 vs. ExPASy Swiss-Prot
Match: P41152 (Heat shock factor protein HSF30 OS=Solanum peruvianum OX=4082 GN=HSF30 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 8.6e-58
Identity = 113/200 (56.50%), Postives = 144/200 (72.00%), Query Frame = 0

Query: 68  PMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSN 127
           PMEGLH+VGP PFL KTYEMVED  TD V+SWS  RNSFIVWDSHK S TLLP++FKHSN
Sbjct: 20  PMEGLHDVGPPPFLSKTYEMVEDSSTDQVISWSTTRNSFIVWDSHKFSTTLLPRFFKHSN 79

Query: 128 FSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQDL 187
           FSSFIRQLNTYGFRK+D D+WEFANEGF GG+KHLLK IKRR        QQ      ++
Sbjct: 80  FSSFIRQLNTYGFRKVDPDRWEFANEGFLGGQKHLLKTIKRRRNVGQSMNQQGSGACIEI 139

Query: 188 SNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVS 247
             + + E EL+ LK D N+L  E++KLR QQQ + N +  + +++   E K  QM+SF++
Sbjct: 140 GYYGM-EEELERLKRDKNVLMTEIVKLRQQQQSTRNQIIAMGEKIETQERKQVQMMSFLA 199

Query: 248 KMSRDPGFCRQLVQRRMLRK 268
           K+  +P F +Q + +++ RK
Sbjct: 200 KIFSNPTFLQQYLDKQVHRK 218

BLAST of Carg10803 vs. ExPASy Swiss-Prot
Match: Q338B0 (Heat stress transcription factor A-2c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2C PE=1 SV=2)

HSP 1 Score: 218.4 bits (555), Expect = 1.4e-55
Identity = 119/237 (50.21%), Postives = 153/237 (64.56%), Query Frame = 0

Query: 34  VKEEEMEVSTETMEGGGGESGGGDGGWASSATAKPMEGLHEVGPAPFLKKTYEMVEDPET 93
           VKEE +E   +  +  GG            A  +PMEGLHEVGP PFL KTY++VEDP T
Sbjct: 9   VKEEMLESQQQQRQEDGG------------AAPRPMEGLHEVGPPPFLTKTYDLVEDPAT 68

Query: 94  DPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANE 153
           D VVSWS   NSF+VWD H  +  LLP+ FKH+NFSSF+RQLNTYGFRK+D D+WEFANE
Sbjct: 69  DGVVSWSRAGNSFVVWDPHVFADLLLPRLFKHNNFSSFVRQLNTYGFRKVDPDRWEFANE 128

Query: 154 GFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQDLSNHSL-VEAELQALKSDNNILRVEML 213
           GF  G++HLLK IKRR   +N    QQ      L       E E+  LK D NIL  E++
Sbjct: 129 GFLRGQRHLLKTIKRRKPPSNAPPSQQQSLTSCLEVGEFGFEEEIDRLKRDKNILITEVV 188

Query: 214 KLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKL 270
           KLR +QQ + +H+  +EDR+R  E K  QM+ F+++  R+P F +QL Q++  RK+L
Sbjct: 189 KLRQEQQATKDHVKAMEDRLRAAEQKQVQMMGFLARAMRNPEFFQQLAQQKEKRKEL 233

BLAST of Carg10803 vs. ExPASy Swiss-Prot
Match: Q9LUH8 (Heat stress transcription factor A-6b OS=Arabidopsis thaliana OX=3702 GN=HSFA6b PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.4e-55
Identity = 106/211 (50.24%), Postives = 155/211 (73.46%), Query Frame = 0

Query: 67  KPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHS 126
           +P+EGLHE GP PFL KTY++VED  T+ VVSWS+  NSFIVWD    S+TLLP++FKH+
Sbjct: 49  QPLEGLHESGPPPFLTKTYDLVEDSRTNHVVSWSKSNNSFIVWDPQAFSVTLLPRFFKHN 108

Query: 127 NFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLG--L 186
           NFSSF+RQLNTYGFRK++ D+WEFANEGF  G+KHLLKNI+RR   NN  + QQ      
Sbjct: 109 NFSSFVRQLNTYGFRKVNPDRWEFANEGFLRGQKHLLKNIRRRKTSNNSNQMQQPQSSEQ 168

Query: 187 QDLSNHSL------VEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESK 246
           Q L N  +      ++ E+ +L+ D  +L +E+++LR QQQ +  +LT++E++++  ESK
Sbjct: 169 QSLDNFCIEVGRYGLDGEMDSLRRDKQVLMMELVRLRQQQQSTKMYLTLIEEKLKKTESK 228

Query: 247 YQQMLSFVSKMSRDPGFCRQLVQRRMLRKKL 270
            +QM+SF+++  ++P F +QLV+++  RK++
Sbjct: 229 QKQMMSFLARAMQNPDFIQQLVEQKEKRKEI 259

BLAST of Carg10803 vs. ExPASy Swiss-Prot
Match: Q6VBB2 (Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2B PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.8e-55
Identity = 116/239 (48.54%), Postives = 161/239 (67.36%), Query Frame = 0

Query: 34  VKEEEMEVSTETMEGGGGESGGGDGGWASSATA---KPMEGLHEVGPAPFLKKTYEMVED 93
           VKEEE        +GGG E   G+ G A+ A     +PMEGLH+ GP PFL KTY+MV+D
Sbjct: 9   VKEEESH-----GDGGGLEVVAGEDGAAAVAAGVAPRPMEGLHDAGPPPFLTKTYDMVDD 68

Query: 94  PETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIRQLNTYGFRKIDRDKWEF 153
             TD  VSWS   NSF+VWD H  +  LLP++FKH+NFSSF+RQLNTYGFRK+D D+WEF
Sbjct: 69  AGTDAAVSWSATSNSFVVWDPHAFATVLLPRFFKHNNFSSFVRQLNTYGFRKVDPDRWEF 128

Query: 154 ANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQDLSNHSLVEAELQALKSDNNILRVE 213
           ANE F  G++HLLKNIKRR   ++    QQ LG      H   +AE+  LK D  +L  E
Sbjct: 129 ANENFLRGQRHLLKNIKRRKPPSHTASNQQSLGPYLEVGHFGYDAEIDRLKRDKQLLMAE 188

Query: 214 MLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKL 270
           ++KLR +QQ++  +L  +EDR++  E + QQM++F++++ ++P F +QL+ +  +RK+L
Sbjct: 189 VVKLRQEQQNTKANLKAMEDRLQGTEQRQQQMMAFLARVMKNPEFLKQLMSQNEMRKEL 242

BLAST of Carg10803 vs. ExPASy TrEMBL
Match: A0A6J1G410 (heat stress transcription factor A-2-like OS=Cucurbita moschata OX=3662 GN=LOC111450541 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 2.3e-207
Identity = 370/372 (99.46%), Postives = 370/372 (99.46%), Query Frame = 0

Query: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60
           MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW
Sbjct: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGESGGGDGGW 60

Query: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120
           ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP
Sbjct: 61  ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLP 120

Query: 121 KYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180
           KYFKHSNFSSFIRQLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ
Sbjct: 121 KYFKHSNFSSFIRQLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQ 180

Query: 181 HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ 240
           HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ
Sbjct: 181 HLGLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQ 240

Query: 241 QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE 300
           QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE
Sbjct: 241 QMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE 300

Query: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCPSGYGQ 360
           MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQK PPPPPPPPQDCPSGYGQ
Sbjct: 301 MIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQK-PPPPPPPPQDCPSGYGQ 360

Query: 361 QQAFHGFVGSIP 373
           QQAFHGFVGSIP
Sbjct: 361 QQAFHGFVGSIP 371

BLAST of Carg10803 vs. ExPASy TrEMBL
Match: A0A6J1KH95 (heat stress transcription factor A-7a-like OS=Cucurbita maxima OX=3661 GN=LOC111493843 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.0e-175
Identity = 327/377 (86.74%), Postives = 339/377 (89.92%), Query Frame = 0

Query: 1   MINQTTLSGGGGGGGGCPDGAATASGDIMDQRTVKEEEMEVSTETMEGGGGES-GGGDGG 60
           MINQT LS    GGGGCPDGAATASGDIMDQRTVKEEE+EVS  TMEGGGGES  GGDG 
Sbjct: 1   MINQTILS---DGGGGCPDGAATASGDIMDQRTVKEEEIEVSAGTMEGGGGESDSGGDGV 60

Query: 61  WASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLL 120
            ASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEG NSFIVWDSHKLSITLL
Sbjct: 61  SASSATAKPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGGNSFIVWDSHKLSITLL 120

Query: 121 PKYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQ 180
           PKYFKHSNFSSFIRQLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKRRSRYNNYK+QQ
Sbjct: 121 PKYFKHSNFSSFIRQLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKQQQ 180

Query: 181 QHL----GLQDLSNHSLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCV 240
            HL    GLQDL+ H LVEAEL ALKSDN +LRVEMLKLR+QQ DS N LTMVEDRVRCV
Sbjct: 181 HHLGMPMGLQDLTKHPLVEAELHALKSDNQVLRVEMLKLREQQHDSQNQLTMVEDRVRCV 240

Query: 241 ESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSE 300
           ESK+QQMLSFVSKMSR+PGFCRQLVQRRMLRKKL+NNGDEFGNNR LLAMQ HQNL+HSE
Sbjct: 241 ESKHQQMLSFVSKMSRNPGFCRQLVQRRMLRKKLMNNGDEFGNNRRLLAMQGHQNLSHSE 300

Query: 301 LITTEMIPQAIEPGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLIQKPPPPPPPPPQDCP 360
           L++TEM P  IEPGP VLE PVR STMVVDEDSDSKFFLELEDLI+K PPPPP   QDCP
Sbjct: 301 LVSTEMFPHVIEPGPAVLEQPVRASTMVVDEDSDSKFFLELEDLIKK-PPPPPTTTQDCP 360

Query: 361 SGYGQQQAFHGFVGSIP 373
           SGY QQQAFHGFVGSIP
Sbjct: 361 SGYVQQQAFHGFVGSIP 373

BLAST of Carg10803 vs. ExPASy TrEMBL
Match: A0A5D3CFL8 (Heat shock factor protein HSF30-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001020 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.8e-112
Identity = 241/382 (63.09%), Postives = 277/382 (72.51%), Query Frame = 0

Query: 19  DGAATASGDIMDQR---TVKEEEM--EVSTETMEGGGGESGGGDGGWASSATAKPMEGLH 78
           DGA T SG+ +D++    VK EE   EVST      GG              AKPMEGLH
Sbjct: 6   DGAETGSGESVDEKMSQVVKGEEEIDEVSTAATMNKGG------------VWAKPMEGLH 65

Query: 79  EVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIR 138
           ++GP PFLKKTYEMVEDPETDPVVSWSE R SFIVWDSH+LS  LLPKYFKHSNFSSFIR
Sbjct: 66  DLGPPPFLKKTYEMVEDPETDPVVSWSETRKSFIVWDSHQLSKFLLPKYFKHSNFSSFIR 125

Query: 139 QLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLG-------LQD 198
           QLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKR+++YNN  K +QHLG       L+D
Sbjct: 126 QLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRKNKYNNNFK-KQHLGLSINNTTLED 185

Query: 199 LSNHSLVEAE-LQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSF 258
           L+   LVE E LQ LK+DNNILRVEM KLR+QQQDSHN LT+VE+RVRC ESK+QQM  F
Sbjct: 186 LTKPLLVETELLQTLKTDNNILRVEMSKLREQQQDSHNQLTLVEERVRCAESKHQQMFYF 245

Query: 259 VSKMSRDPGFCRQLVQRRMLRKKL-INNGD-EFGNNRSLLAMQAHQNLTHSELIT----- 318
           ++KMSR+P FCRQL+Q+RMLRKK+ ++NGD EFG  R LLA+QAHQN    EL T     
Sbjct: 246 LAKMSRNPAFCRQLIQKRMLRKKMELSNGDHEFGKKRKLLAVQAHQNQVQEELSTLHSEL 305

Query: 319 TEMIPQAIEPGPVVLEPPVRPS-------TMVVDED-SDSKFFLELEDLIQKPPPPPPPP 373
           TEM P+ IEPGP  +    R S        MVVDE  S +  FL+L+DLI+K        
Sbjct: 306 TEMFPEVIEPGPGPIGTVFRESIDDDISENMVVDEGLSSNDSFLKLDDLIKK-------- 365

BLAST of Carg10803 vs. ExPASy TrEMBL
Match: A0A1S3CAP8 (heat shock factor protein HSF30-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498575 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.8e-112
Identity = 241/382 (63.09%), Postives = 277/382 (72.51%), Query Frame = 0

Query: 19  DGAATASGDIMDQR---TVKEEEM--EVSTETMEGGGGESGGGDGGWASSATAKPMEGLH 78
           DGA T SG+ +D++    VK EE   EVST      GG              AKPMEGLH
Sbjct: 6   DGAETGSGESVDEKMSQVVKGEEEIDEVSTAATMNKGG------------VWAKPMEGLH 65

Query: 79  EVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIR 138
           ++GP PFLKKTYEMVEDPETDPVVSWSE R SFIVWDSH+LS  LLPKYFKHSNFSSFIR
Sbjct: 66  DLGPPPFLKKTYEMVEDPETDPVVSWSETRKSFIVWDSHQLSKFLLPKYFKHSNFSSFIR 125

Query: 139 QLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLG-------LQD 198
           QLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKR+++YNN  K +QHLG       L+D
Sbjct: 126 QLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRKNKYNNNFK-KQHLGLSINNTTLED 185

Query: 199 LSNHSLVEAE-LQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSF 258
           L+   LVE E LQ LK+DNNILRVEM KLR+QQQDSHN LT+VE+RVRC ESK+QQM  F
Sbjct: 186 LTKPLLVETELLQTLKTDNNILRVEMSKLREQQQDSHNQLTLVEERVRCAESKHQQMFYF 245

Query: 259 VSKMSRDPGFCRQLVQRRMLRKKL-INNGD-EFGNNRSLLAMQAHQNLTHSELIT----- 318
           ++KMSR+P FCRQL+Q+RMLRKK+ ++NGD EFG  R LLA+QAHQN    EL T     
Sbjct: 246 LAKMSRNPAFCRQLIQKRMLRKKMELSNGDHEFGKKRKLLAVQAHQNQVQEELSTLHSEL 305

Query: 319 TEMIPQAIEPGPVVLEPPVRPS-------TMVVDED-SDSKFFLELEDLIQKPPPPPPPP 373
           TEM P+ IEPGP  +    R S        MVVDE  S +  FL+L+DLI+K        
Sbjct: 306 TEMFPEVIEPGPGPIGTVFRESIDDDRSENMVVDEGLSSNDSFLKLDDLIKK-------- 365

BLAST of Carg10803 vs. ExPASy TrEMBL
Match: A0A5A7V4T3 (Heat shock factor protein HSF30-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00460 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 4.1e-111
Identity = 240/382 (62.83%), Postives = 276/382 (72.25%), Query Frame = 0

Query: 19  DGAATASGDIMDQR---TVKEEEM--EVSTETMEGGGGESGGGDGGWASSATAKPMEGLH 78
           D A T SG+ +D++    VK EE   EVST      GG              AKPMEGLH
Sbjct: 6   DRAETGSGESVDEKMSQVVKGEEEIDEVSTAATMNKGG------------VWAKPMEGLH 65

Query: 79  EVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIR 138
           ++GP PFLKKTYEMVEDPETDPVVSWSE R SFIVWDSH+LS  LLPKYFKHSNFSSFIR
Sbjct: 66  DLGPPPFLKKTYEMVEDPETDPVVSWSETRKSFIVWDSHQLSKFLLPKYFKHSNFSSFIR 125

Query: 139 QLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLG-------LQD 198
           QLNTYGFRKID DKWEFANEGFQGGKKHLLKNIKR+++YNN  K +QHLG       L+D
Sbjct: 126 QLNTYGFRKIDSDKWEFANEGFQGGKKHLLKNIKRKNKYNNNFK-KQHLGLSINNTTLED 185

Query: 199 LSNHSLVEAE-LQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSF 258
           L+   LVE E LQ LK+DNNILRVEM KLR+QQQDSHN LT+VE+RVRC ESK+QQM  F
Sbjct: 186 LTKPLLVETELLQTLKTDNNILRVEMSKLREQQQDSHNQLTLVEERVRCAESKHQQMFYF 245

Query: 259 VSKMSRDPGFCRQLVQRRMLRKKL-INNGD-EFGNNRSLLAMQAHQNLTHSELIT----- 318
           ++KMSR+P FCRQL+Q+RMLRKK+ ++NGD EFG  R LLA+QAHQN    EL T     
Sbjct: 246 LAKMSRNPAFCRQLIQKRMLRKKMELSNGDHEFGKKRKLLAVQAHQNQVQEELSTLHSEL 305

Query: 319 TEMIPQAIEPGPVVLEPPVRPS-------TMVVDED-SDSKFFLELEDLIQKPPPPPPPP 373
           TEM P+ IEPGP  +    R S        MVVDE  S +  FL+L+DLI+K        
Sbjct: 306 TEMFPEVIEPGPGPIGTVFRESIDDDRSENMVVDEGLSSNDSFLKLDDLIKK-------- 365

BLAST of Carg10803 vs. TAIR 10
Match: AT2G26150.1 (heat shock transcription factor A2 )

HSP 1 Score: 226.9 bits (577), Expect = 2.7e-59
Identity = 128/268 (47.76%), Postives = 172/268 (64.18%), Query Frame = 0

Query: 34  VKEEEMEVSTETMEGGGGESGGGDGGWASSATAKPMEGLHEVGPAPFLKKTYEMVEDPET 93
           ++E ++E+  ET+   G  +     G  SS++ +PMEGL+E GP PFL KTYEMVEDP T
Sbjct: 1   MEELKVEMEEETVTFTGSVAASSSVG--SSSSPRPMEGLNETGPPPFLTKTYEMVEDPAT 60

Query: 94  DPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIRQLNTYGFRKIDRDKWEFANE 153
           D VVSWS GRNSF+VWDSHK S TLLP+YFKHSNFSSFIRQLNTYGFRKID D+WEFANE
Sbjct: 61  DTVVSWSNGRNSFVVWDSHKFSTTLLPRYFKHSNFSSFIRQLNTYGFRKIDPDRWEFANE 120

Query: 154 GFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQ--DLSNHSLVEAELQALKSDNNILRVEM 213
           GF  G+KHLLKNIKRR         QQ  G+   ++  +   + E++ LK D+ +L  E+
Sbjct: 121 GFLAGQKHLLKNIKRRRNMGLQNVNQQGSGMSCVEVGQYGF-DGEVERLKRDHGVLVAEV 180

Query: 214 LKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMSRDPGFCRQLVQRRMLRKKLIN 273
           ++LR QQ  S + +  +E R+   E + QQM++F++K   +P F +Q       +K L  
Sbjct: 181 VRLRQQQHSSKSQVAAMEQRLLVTEKRQQQMMTFLAKALNNPNFVQQFAVMSKEKKSLF- 240

Query: 274 NGDEFGNNRSLLAMQA----HQNLTHSE 296
            G + G  R L +  +     +NL H +
Sbjct: 241 -GLDVGRKRRLTSTPSLGTMEENLLHDQ 263

BLAST of Carg10803 vs. TAIR 10
Match: AT3G22830.1 (heat shock transcription factor A6B )

HSP 1 Score: 218.4 bits (555), Expect = 9.7e-57
Identity = 106/211 (50.24%), Postives = 155/211 (73.46%), Query Frame = 0

Query: 67  KPMEGLHEVGPAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHS 126
           +P+EGLHE GP PFL KTY++VED  T+ VVSWS+  NSFIVWD    S+TLLP++FKH+
Sbjct: 49  QPLEGLHESGPPPFLTKTYDLVEDSRTNHVVSWSKSNNSFIVWDPQAFSVTLLPRFFKHN 108

Query: 127 NFSSFIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLG--L 186
           NFSSF+RQLNTYGFRK++ D+WEFANEGF  G+KHLLKNI+RR   NN  + QQ      
Sbjct: 109 NFSSFVRQLNTYGFRKVNPDRWEFANEGFLRGQKHLLKNIRRRKTSNNSNQMQQPQSSEQ 168

Query: 187 QDLSNHSL------VEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESK 246
           Q L N  +      ++ E+ +L+ D  +L +E+++LR QQQ +  +LT++E++++  ESK
Sbjct: 169 QSLDNFCIEVGRYGLDGEMDSLRRDKQVLMMELVRLRQQQQSTKMYLTLIEEKLKKTESK 228

Query: 247 YQQMLSFVSKMSRDPGFCRQLVQRRMLRKKL 270
            +QM+SF+++  ++P F +QLV+++  RK++
Sbjct: 229 QKQMMSFLARAMQNPDFIQQLVEQKEKRKEI 259

BLAST of Carg10803 vs. TAIR 10
Match: AT5G54070.1 (heat shock transcription factor A9 )

HSP 1 Score: 212.6 bits (540), Expect = 5.3e-55
Identity = 119/273 (43.59%), Postives = 174/273 (63.74%), Query Frame = 0

Query: 72  LHEVG-PAPFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSS 131
           LHE+G   PFL+KT+E+V+D  TDPVVSWS  R SFI+WDS++ S  LLPKYFKH NFSS
Sbjct: 63  LHEIGLITPFLRKTFEIVDDKVTDPVVSWSPTRKSFIIWDSYEFSENLLPKYFKHKNFSS 122

Query: 132 FIRQLNTYGFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQDLSNH 191
           FIRQLN+YGF+K+D D+WEFANEGFQGGKKHLLKNIKRRS+      ++        ++ 
Sbjct: 123 FIRQLNSYGFKKVDSDRWEFANEGFQGGKKHLLKNIKRRSKNTKCCNKE--------AST 182

Query: 192 SLVEAELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMS 251
           +  E E+++LK + + +R+EMLKL+ QQ++S + +  V++++  V+++ Q MLSF +K++
Sbjct: 183 TTTETEVESLKEEQSPMRLEMLKLKQQQEESQHQMVTVQEKIHGVDTEQQHMLSFFAKLA 242

Query: 252 RDPGFCRQLVQRRMLRKKLINNGDEFGNNRSLLAMQAHQNLTHSELITTE---MIPQAIE 311
           +D  F  +LV++R ++ +      EF     LL  Q     T   L+  E   M   A E
Sbjct: 243 KDQRFVERLVKKRKMKIQRELEAAEFVKKLKLLQDQE----TQKNLLDVEREFMAMAATE 302

Query: 312 PGPVVLEPPVRPSTMVVDEDSDSKFFLELEDLI 341
             P        P  +V ++  +++  L  EDL+
Sbjct: 303 HNP-------EPDILVNNQSGNTRCQLNSEDLL 316

BLAST of Carg10803 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 190.7 bits (483), Expect = 2.2e-48
Identity = 114/251 (45.42%), Postives = 154/251 (61.35%), Query Frame = 0

Query: 79  PFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIRQLNTY 138
           PFL KTY+MV+DP T+ VVSWS G NSF+VW + + S  LLPKYFKH+NFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 139 GFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQDLSNHSLVEA--- 198
           GFRK+D D+WEFANEGF  G+K LLK+I RR + ++ ++ QQ   +Q  S  + VE    
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRR-KPSHVQQNQQQTQVQSSSVGACVEVGKF 146

Query: 199 ----ELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMSR 258
               E++ LK D N+L  E+++LR QQQ + N L  V  +V+ +E + QQM+SF++K  +
Sbjct: 147 GIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQ 206

Query: 259 DPGFCRQLVQ----------------RRMLRKKLINNGDEFGN--NRSLLAMQ-----AH 300
            PGF  QLVQ                RR+   +  N GD   N  NR ++  Q     A 
Sbjct: 207 SPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSINEAA 266

BLAST of Carg10803 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 190.7 bits (483), Expect = 2.2e-48
Identity = 114/251 (45.42%), Postives = 154/251 (61.35%), Query Frame = 0

Query: 79  PFLKKTYEMVEDPETDPVVSWSEGRNSFIVWDSHKLSITLLPKYFKHSNFSSFIRQLNTY 138
           PFL KTY+MV+DP T+ VVSWS G NSF+VW + + S  LLPKYFKH+NFSSF+RQLNTY
Sbjct: 27  PFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNTY 86

Query: 139 GFRKIDRDKWEFANEGFQGGKKHLLKNIKRRSRYNNYKKQQQHLGLQDLSNHSLVEA--- 198
           GFRK+D D+WEFANEGF  G+K LLK+I RR + ++ ++ QQ   +Q  S  + VE    
Sbjct: 87  GFRKVDPDRWEFANEGFLRGRKQLLKSIVRR-KPSHVQQNQQQTQVQSSSVGACVEVGKF 146

Query: 199 ----ELQALKSDNNILRVEMLKLRDQQQDSHNHLTMVEDRVRCVESKYQQMLSFVSKMSR 258
               E++ LK D N+L  E+++LR QQQ + N L  V  +V+ +E + QQM+SF++K  +
Sbjct: 147 GIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAVQ 206

Query: 259 DPGFCRQLVQ----------------RRMLRKKLINNGDEFGN--NRSLLAMQ-----AH 300
            PGF  QLVQ                RR+   +  N GD   N  NR ++  Q     A 
Sbjct: 207 SPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSINEAA 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030489.17.9e-210100.00Heat shock factor protein HSF30, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022946498.14.8e-20799.46heat stress transcription factor A-2-like [Cucurbita moschata][more]
KAG6599513.12.2e-20497.85Heat shock factor protein HSF30, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023546638.12.7e-18690.36heat stress transcription factor A-2-like [Cucurbita pepo subsp. pepo][more]
XP_022999504.18.3e-17586.74heat stress transcription factor A-7a-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O809823.9e-5847.76Heat stress transcription factor A-2 OS=Arabidopsis thaliana OX=3702 GN=HSFA2 PE... [more]
P411528.6e-5856.50Heat shock factor protein HSF30 OS=Solanum peruvianum OX=4082 GN=HSF30 PE=2 SV=1[more]
Q338B01.4e-5550.21Heat stress transcription factor A-2c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LUH81.4e-5550.24Heat stress transcription factor A-6b OS=Arabidopsis thaliana OX=3702 GN=HSFA6b ... [more]
Q6VBB26.8e-5548.54Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
A0A6J1G4102.3e-20799.46heat stress transcription factor A-2-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1KH954.0e-17586.74heat stress transcription factor A-7a-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3CFL84.8e-11263.09Heat shock factor protein HSF30-like isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3CAP84.8e-11263.09heat shock factor protein HSF30-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7V4T34.1e-11162.83Heat shock factor protein HSF30-like isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
AT2G26150.12.7e-5947.76heat shock transcription factor A2 [more]
AT3G22830.19.7e-5750.24heat shock transcription factor A6B [more]
AT5G54070.15.3e-5543.59heat shock transcription factor A9 [more]
AT5G16820.12.2e-4845.42heat shock factor 3 [more]
AT5G16820.22.2e-4845.42heat shock factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 197..217
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 340..355
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 338..362
NoneNo IPR availablePANTHERPTHR10015:SF298HEAT STRESS TRANSCRIPTION FACTOR A-9coord: 43..291
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 80..103
score: 47.02
coord: 118..130
score: 78.02
coord: 131..143
score: 81.76
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 76..169
e-value: 2.0E-56
score: 203.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 80..169
e-value: 1.4E-31
score: 108.9
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 119..143
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 73..170
e-value: 2.4E-36
score: 126.3
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 43..291
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 76..169

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10803-RACarg10803-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding