Carg10793 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10793
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	thermospermine synthase ACAULIS5-like
Location	Carg_Chr05: 12057610 .. 12060045 (+)
RNA-Seq Expression	Carg10793
Synteny	Carg10793

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTCTCCTCTTTCAATTCAATTTCCCACAATGCTTTCACCCCTCTGCTCTGCCCTCCCTGCTCTCTCTATCCCCACCTCCAACTCCACTGCTGTTCCCCATGTCTCTGAATGGTTCGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGTCCATTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTCTCCTGCCCATTACGTGTTTGTTAAAATGTCTTCCCCTTCTTTCCTCATCTAATTCATCTCCTGTTTCCCGGAGTTCTTTCACTGTAGACTGCATGAACCAAATTCAACAAATTCTAACGAATTCTAACAAATTGTGACATTCCATTCCTTTAGGATACTTCATGCTGCAACCAGCAAGTTCCAAGATATAGTTCTGCTGGACACCAAGCGATTTGGGAAGGTTTGCATGATCCCACATCACTTCAATTTCCTTTCCGACTACACTTTATTCATTTAATTCAAAGCTTTTCAGGCTCTGTTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAGTTCATTTATCATGAGTCCATGGTTCATCCTGCTCTGTTACTGCACCACAAGTATACACGCTGTGTTCATATTTGGTTTACTTCTTTTTAACTATTCTTATTGTGGGTACTGAAATTCTTTTGGTTTTGTGAACGATTTCAGCCCCAAAACAGTGTTTATACTGGGAGGTGGAGAAGGGTCTACTGCTAGAGAGACACTAAAGCACAAAAGTATTGAGAAAGTTGTGATGTGCGATATTGATAGGGTATGTCTCAAGATACACTCAGTTTAGTTTATATATTTTCAAGTGGTAGAAGATAAGTGAAGTGTTGTAACGGTCAAGCCCACCGCTAGTAGATATTGTGTTTTTTGGACTTTTCCTCTTGGGCTTCCTCCACACCCTTATGTGAGATATCACAATCCCCTACAACGTTCTCGCTATTACTCGTTTCTTTCTCCCATTGATGTGGGACTCCCACCAAATGCACTCCCTTCAGGGCCAGCGTCCTTACTAACACACTGCCTTGTGTCTATCCCCTTTAGGGAACAACCTCCTTGCTGGCACATTGCCTAGTGTTTGGCTTTGATATCATTTGTAATGGTCAAGCCTACCGCTAGGCTTTGATATCATTTGTAATGGTCAAGCCCACCGCTAGCCGATATTGTCCTCTTTGAGCTTTCCCTTTCAGGCTTCCCCTCAAGGCTTTAGGGAAAGGTTTCCACACCCTTATAAACTGTATTTTGTTCTCCTCGCTAACCAATGTGTGATATCACAAGTGTAGTTCATATTAACCGATGCGTGTTCATATTGTAGGATGTTGTCAATTTCTGCCGCGCACATCTGAGCGAAAATCAAGATGCATTTCAGGATGAGAAGCTTCATATCATTTTTGATGATGCAAAGTGAGTAGTTTATATCTCAACTTTTACTTGAATGAATTTCAATATTTCAAAAGGGTTAACCTGAAAACTTAAAAAGGGTGGGATTGGAGGGACAACCCGAGAAGTTCGACATAATCATCAGCGATTTAGCCGATCCACATGAAGGAGGACCTTGCAATCATCTGTACACTAAATCATTCTACGAGGAAGGGATTAAGCCCAAGCTGAATGACAATGGTATCTTTGTTACTCAGGTACATTATATAACATATTCAAGCCCCTCTGCTATTGTTACTGAGATTTAACTTAAGGGTGTCCTAAAATGCAGGCTGGCCCTTCTGGTATTCTCTCCTACAATGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAAATGTAAGACAACTTTCCCTTGATGCATTTGTAGAGCTAACCTTTTGTTTTGAAACAAGGCTTCTTAGATGAACAAGTTCATGTAGATCTAAACTTGAAGCTCTCTGTTTGGTCTAACATCTTTCATTTTATGTGCGCAGATGTGATCGCATACACCGCTCATATTCCATCCTATGCAGATTCGAGCGGATGGGTTCTGGTAAGCAACTAGAAAGAGTTCGAATGACTTTCTAAGTGCTTAAAAATATTTACTTTTTAAACCGTTGAAAAGTCTGAGAGATGTCACGTTATAATATGGTTTTATACAAAATTGTAATGATAGGCTAGTGATCATCCTTTTAAACTGGACGTCGAAGATCTCGACAAGAGGATACGTGAGAGAGTTCGAGGCGAGCTACGCTACTTGGACGGTGCATTCATCGTGTCATCAACAGTCATAAACAAGACCATTCGTACAGCGTAAGTACAAAGATAGTTGACTTCCATCGTCTCGATTCGTTGAGCGGAAAAAAAAAGAAAAAAATCATGTTTGATGATGTTGTGGTTGTGCGTGCAGGATGATGAATGAGACTCATGTGTTCACTGAGGAAGATGCGAGGTTCACTCATGGCCAAGGGCTGGCTAGCCATGCCTAG

mRNA sequence

CATTCTCCTCTTTCAATTCAATTTCCCACAATGCTTTCACCCCTCTGCTCTGCCCTCCCTGCTCTCTCTATCCCCACCTCCAACTCCACTGCTGTTCCCCATGTCTCTGAATGGTTCGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGATACTTCATGCTGCAACCAGCAAGTTCCAAGATATAGTTCTGCTGGACACCAAGCGATTTGGGAAGGCTCTGTTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAGTTCATTTATCATGAGTCCATGGTTCATCCTGCTCTGTTACTGCACCACAACCCCAAAACAGTGTTTATACTGGGAGGTGGAGAAGGGTCTACTGCTAGAGAGACACTAAAGCACAAAAGTATTGAGAAAGTTGTGATGTGCGATATTGATAGGGATGTTGTCAATTTCTGCCGCGCACATCTGAGCGAAAATCAAGATGCATTTCAGGATGAGAAGCTTCATATCATTTTTGATGATGCAAAGGTGGGATTGGAGGGACAACCCGAGAAGTTCGACATAATCATCAGCGATTTAGCCGATCCACATGAAGGAGGACCTTGCAATCATCTGTACACTAAATCATTCTACGAGGAAGGGATTAAGCCCAAGCTGAATGACAATGGTATCTTTGTTACTCAGGCTGGCCCTTCTGGTATTCTCTCCTACAATGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAAATATGTGATCGCATACACCGCTCATATTCCATCCTATGCAGATTCGAGCGGATGGGTTCTGGCTAGTGATCATCCTTTTAAACTGGACGTCGAAGATCTCGACAAGAGGATACGTGAGAGAGTTCGAGGCGAGCTACGCTACTTGGACGGTGCATTCATCGTGTCATCAACAGTCATAAACAAGACCATTCGTACAGCGATGATGAATGAGACTCATGTGTTCACTGAGGAAGATGCGAGGTTCACTCATGGCCAAGGGCTGGCTAGCCATGCCTAG

Coding sequence (CDS)

ATGCTTTCACCCCTCTGCTCTGCCCTCCCTGCTCTCTCTATCCCCACCTCCAACTCCACTGCTGTTCCCCATGTCTCTGAATGGTTCGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGATACTTCATGCTGCAACCAGCAAGTTCCAAGATATAGTTCTGCTGGACACCAAGCGATTTGGGAAGGCTCTGTTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAGTTCATTTATCATGAGTCCATGGTTCATCCTGCTCTGTTACTGCACCACAACCCCAAAACAGTGTTTATACTGGGAGGTGGAGAAGGGTCTACTGCTAGAGAGACACTAAAGCACAAAAGTATTGAGAAAGTTGTGATGTGCGATATTGATAGGGATGTTGTCAATTTCTGCCGCGCACATCTGAGCGAAAATCAAGATGCATTTCAGGATGAGAAGCTTCATATCATTTTTGATGATGCAAAGGTGGGATTGGAGGGACAACCCGAGAAGTTCGACATAATCATCAGCGATTTAGCCGATCCACATGAAGGAGGACCTTGCAATCATCTGTACACTAAATCATTCTACGAGGAAGGGATTAAGCCCAAGCTGAATGACAATGGTATCTTTGTTACTCAGGCTGGCCCTTCTGGTATTCTCTCCTACAATGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAAATATGTGATCGCATACACCGCTCATATTCCATCCTATGCAGATTCGAGCGGATGGGTTCTGGCTAGTGATCATCCTTTTAAACTGGACGTCGAAGATCTCGACAAGAGGATACGTGAGAGAGTTCGAGGCGAGCTACGCTACTTGGACGGTGCATTCATCGTGTCATCAACAGTCATAAACAAGACCATTCGTACAGCGATGATGAATGAGACTCATGTGTTCACTGAGGAAGATGCGAGGTTCACTCATGGCCAAGGGCTGGCTAGCCATGCCTAG

Protein sequence

MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARFTHGQGLASHA

Homology

[Close]

BLAST of Carg10793 vs. NCBI nr
Match: KAG7030499.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 672.5 bits (1734), Expect = 1.8e-189
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60
           MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60

Query: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120
           DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH
Sbjct: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120

Query: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180
           KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL
Sbjct: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180

Query: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240
           ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY
Sbjct: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240

Query: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300
           VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK
Sbjct: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300

Query: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           TIRTAMMNETHVFTEEDARFTHGQGLASHA
Sbjct: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Carg10793 vs. NCBI nr
Match: XP_022946737.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 671.8 bits (1732), Expect = 3.1e-189
Identity = 328/330 (99.39%), Postives = 330/330 (100.00%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60
           MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60

Query: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120
           DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH
Sbjct: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120

Query: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180
           KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFD+IISDL
Sbjct: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVIISDL 180

Query: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240
           ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY
Sbjct: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240

Query: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300
           VIAYTAHIPSYADSSGWVLASDHPFKLD+EDLDKRIRERVRGELRYLDGAFIVSSTVINK
Sbjct: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300

Query: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           TIRTAMMNETHVFTEEDARFTHGQGLASHA
Sbjct: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Carg10793 vs. NCBI nr
Match: KAG6599521.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667.9 bits (1722), Expect = 4.4e-188
Identity = 328/330 (99.39%), Postives = 328/330 (99.39%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60
           MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60

Query: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120
           DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH
Sbjct: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120

Query: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180
           KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL
Sbjct: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180

Query: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240
           ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY
Sbjct: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240

Query: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300
           VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK
Sbjct: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300

Query: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           TIRTAMMNETHV TEEDARF HGQGLASHA
Sbjct: 301 TIRTAMMNETHVITEEDARFIHGQGLASHA 330

[Close]

BLAST of Carg10793 vs. NCBI nr
Match: XP_022999223.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 662.1 bits (1707), Expect = 2.4e-186
Identity = 324/330 (98.18%), Postives = 328/330 (99.39%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60
           MLSPLCSALPALSIPTSNSTAVP VSEWFEEQLEDDLKWSFAV RILHAATSKFQDIVLL
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPQVSEWFEEQLEDDLKWSFAVTRILHAATSKFQDIVLL 60

Query: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120
           DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH
Sbjct: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120

Query: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180
           KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIF+DAKVGLEGQPEKFD+IISDL
Sbjct: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDVIISDL 180

Query: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240
           ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY
Sbjct: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240

Query: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300
           VIAYTAHIPSYADSSGWVLASDHPF+LD+EDLDKRIRERVRGELRYLDGAFIVSSTVINK
Sbjct: 241 VIAYTAHIPSYADSSGWVLASDHPFELDMEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300

Query: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           TIRTAMMNETHVFTEEDARFTHGQGLASHA
Sbjct: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Carg10793 vs. NCBI nr
Match: XP_023546321.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 661.4 bits (1705), Expect = 4.1e-186
Identity = 324/330 (98.18%), Postives = 327/330 (99.09%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60
           MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60

Query: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120
           DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH
Sbjct: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120

Query: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180
           KSIEKVVMCDIDRDVVNFC AHLSENQDAFQDEKLHIIF+DAKVGLE QPEKFD+IISDL
Sbjct: 121 KSIEKVVMCDIDRDVVNFCLAHLSENQDAFQDEKLHIIFEDAKVGLERQPEKFDVIISDL 180

Query: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240
           ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY
Sbjct: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240

Query: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300
           VIAYTAHIPSYADSSGWVLASDHPFKLD+EDLDKRI ERVRGELRYLDGAFIVSSTVINK
Sbjct: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRICERVRGELRYLDGAFIVSSTVINK 300

Query: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           TIRTAMMNETHVFTEEDARFTHGQGLASHA
Sbjct: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Carg10793 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 5.5e-117
Identity = 197/314 (62.74%), Postives = 249/314 (79.30%), Query Frame = 0


Query: 15  PTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLDGK 74
           PT    +      W+EE ++DDLKWSFA+N +LH  TS++QDI LLDTKRFGK L++DGK
Sbjct: 22  PTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGK 81

Query: 75  LQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDIDRD 134
           +QSAE+DEFIYHE ++HPALL H NPKTVFI+GGGEGS ARE LKH +IEKVVMCDID++
Sbjct: 82  MQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQE 141

Query: 135 VVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDLADPHEGGPCNHLYT 194
           VV+FCR  L+ N DAF ++KL ++  DAK  LE + EKFDII+ DLADP EGGPC  LYT
Sbjct: 142 VVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYT 201

Query: 195 KSFYEEGIKPKLNDNGIFVTQAGPSGILSY-NVFSSIYNTVKHVFKYVIAYTAHIPSYAD 254
           KSFY+  +KPKL+ NGIFVTQAGP+GI ++  VF+SIYNT+K VFKYV AYTAH+PS+AD
Sbjct: 202 KSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFAD 261

Query: 255 SSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVF 314
           + GWV+ASDH F ++V+++D+RI ERV GEL YL+    VS+  +NKTI  A+  ET V+
Sbjct: 262 TWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVY 321

Query: 315 TEEDARFTHGQGLA 328
           +EE+ARF HG G+A
Sbjct: 322 SEENARFIHGHGVA 335

[Close]

BLAST of Carg10793 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 117/279 (41.94%), Postives = 172/279 (61.65%), Query Frame = 0


Query: 42  AVNRILHAATSKFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPK 101
           A+ +++    + +Q  +L++  RFGKAL+LDGK+QS   DEFIYHE++VHP LL  +NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 102 TVFILGGGEGSTARETLKHKSIEKVVMCDIDRDVVNFCRAHLSE-NQDAFQDEKLHIIFD 161
            + ILGGGEG+T RE LKHK+I+ V M DID  V++F + +L E +Q AF + K  ++ +
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 162 DAKVGLEGQPEKFDIIISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSG 221
           D    ++   EKFD ++ DL DP +  P   LYTK FYEE    +++  GI      PS 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEE--VKRISKWGIVTQATSPS- 199

Query: 222 ILSYNVFSSIYNTVKHVFKYVIAYTAHIPSYADSSGWVLASD--HPFKLDVEDLDKRIRE 281
             S   FS IYNT+KHVFK V A   ++P++    G+V ASD  +P +   E+++ RI+E
Sbjct: 200 -FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIKE 259

Query: 282 RVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEED 318
           R+ G LR+ DG   ++   I K IR  +  E  + T E+
Sbjct: 260 RIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTREN 294

[Close]

BLAST of Carg10793 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 1.0e-54
Identity = 123/300 (41.00%), Postives = 179/300 (59.67%), Query Frame = 0


Query: 28  WFEEQLEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHE 87
           WF E L  D      +  ++    S+FQ+I ++     GK L+LDGK QS+E DEF+YHE
Sbjct: 15  WFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHE 74

Query: 88  SMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDIDRDVVNFCRAHLSE-N 147
           ++VHPA++LH +P+ V ILGGGEG+T RE LKH+S+EK VM DID  VVN  R HL E +
Sbjct: 75  ALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREHLREWH 134

Query: 148 QDAFQDEKLHIIFDDAKVGLEGQPEK-FDIIISDLADPHEGGPCNHLYTKSFYEEGIKPK 207
           + AF D +  ++ DDA   +  + E  FD++I+DL DP E GP   LY++ +Y   +K  
Sbjct: 135 RGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYYRM-VKDV 194

Query: 208 LNDNGIFVTQA-GPSGILSYNVFSSIYNTVKHVFKYVIAYTAHIPSYADSSGWVLASDHP 267
           +N  G+FVTQA   S +  Y+    I NTV  VFK+V +Y  ++PS+    G+V+ASD  
Sbjct: 195 MNPGGVFVTQAVSISHLTEYHAI--IRNTVARVFKHVESYGVYVPSFDSMWGFVVASDDK 254

Query: 268 FKLDVED---LDKRIRERVRG-ELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARF 321
               + D    + R+  +++G ELR+LD A ++    I K  R A+  E    T ++  F
Sbjct: 255 DPRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIAREKRYATLDNQVF 311

[Close]

BLAST of Carg10793 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.7e-54
Identity = 116/292 (39.73%), Postives = 174/292 (59.59%), Query Frame = 0


Query: 43  VNRILHAATSKFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKT 102
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE++VHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 103 VFILGGGEGSTARETLKHKSIEKVVMCDIDRDVVNFCRAHLSE-NQDAFQDEKLHIIFDD 162
           V I+GGGEG+T RE LKH ++EK VM DID ++V   + H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 163 AKVGLEGQPEKFDIIISDLADP-HEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSG 222
           A+  LE   E++D++I DL DP  E  P   LYT  FY   +K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYRL-VKAHLNPGGVMGMQAGMIL 200

Query: 223 ILSYNVFSSIYNTVKHVFKYVIAYTAHIPSYADSSGWVLASD--HPFKLDVEDLDKRIRE 282
           +  + V   ++ TV+  F+YV +Y  HIP +  + G++LASD   P       ++ RIRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 283 RVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           R    LR+L   ++ +  V+ K +  A+  ET V T+++  +   +G A  A
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEGEARQA 310

[Close]

BLAST of Carg10793 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.7e-54
Identity = 116/292 (39.73%), Postives = 174/292 (59.59%), Query Frame = 0


Query: 43  VNRILHAATSKFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKT 102
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE++VHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 103 VFILGGGEGSTARETLKHKSIEKVVMCDIDRDVVNFCRAHLSE-NQDAFQDEKLHIIFDD 162
           V I+GGGEG+T RE LKH ++EK VM DID ++V   + H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 163 AKVGLEGQPEKFDIIISDLADP-HEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSG 222
           A+  LE   E++D++I DL DP  E  P   LYT  FY   +K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYRL-VKAHLNPGGVMGMQAGMIL 200

Query: 223 ILSYNVFSSIYNTVKHVFKYVIAYTAHIPSYADSSGWVLASD--HPFKLDVEDLDKRIRE 282
           +  + V   ++ TV+  F+YV +Y  HIP +  + G++LASD   P       ++ RIRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 283 RVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           R    LR+L   ++ +  V+ K +  A+  ET V T+++  +   +G A  A
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEGEARQA 310

[Close]

BLAST of Carg10793 vs. ExPASy TrEMBL
Match: A0A6J1G4J8 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450716 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.5e-189
Identity = 328/330 (99.39%), Postives = 330/330 (100.00%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60
           MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60

Query: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120
           DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH
Sbjct: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120

Query: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180
           KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFD+IISDL
Sbjct: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVIISDL 180

Query: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240
           ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY
Sbjct: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240

Query: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300
           VIAYTAHIPSYADSSGWVLASDHPFKLD+EDLDKRIRERVRGELRYLDGAFIVSSTVINK
Sbjct: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300

Query: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           TIRTAMMNETHVFTEEDARFTHGQGLASHA
Sbjct: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Carg10793 vs. ExPASy TrEMBL
Match: A0A6J1KJ05 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111493662 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.2e-186
Identity = 324/330 (98.18%), Postives = 328/330 (99.39%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLL 60
           MLSPLCSALPALSIPTSNSTAVP VSEWFEEQLEDDLKWSFAV RILHAATSKFQDIVLL
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPQVSEWFEEQLEDDLKWSFAVTRILHAATSKFQDIVLL 60

Query: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120
           DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH
Sbjct: 61  DTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKH 120

Query: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDL 180
           KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIF+DAKVGLEGQPEKFD+IISDL
Sbjct: 121 KSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDVIISDL 180

Query: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240
           ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY
Sbjct: 181 ADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKY 240

Query: 241 VIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300
           VIAYTAHIPSYADSSGWVLASDHPF+LD+EDLDKRIRERVRGELRYLDGAFIVSSTVINK
Sbjct: 241 VIAYTAHIPSYADSSGWVLASDHPFELDMEDLDKRIRERVRGELRYLDGAFIVSSTVINK 300

Query: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           TIRTAMMNETHVFTEEDARFTHGQGLASHA
Sbjct: 301 TIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Carg10793 vs. ExPASy TrEMBL
Match: A0A0A0LET5 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.3e-163
Identity = 278/333 (83.48%), Postives = 305/333 (91.59%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHVS---EWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60
           M S  CSAL  L +P S+S  + H+    +WFEE+LEDDLKWSFAVNR+LHA TS+FQDI
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHL ENQDAFQDE+LHIIFDDAK GLEG+PEKFD+II
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYE+ IKPKL+DNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLASDHP KLD+EDL+ +IRERV+GEL YLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           INKTIRT MMNETHVFTEEDARF HG+GL ++A
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 333

[Close]

BLAST of Carg10793 vs. ExPASy TrEMBL
Match: A0A5A7UZV1 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001170 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.1e-163
Identity = 281/333 (84.38%), Postives = 303/333 (90.99%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHV---SEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60
           M S  CSAL  L IP S+S  + H+    +WFEE+LEDDLKWSFAVNR+LHA TS+FQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHL ENQDAFQDEKLHIIFDDAK GLEG+ EKFD+II
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYE+ IKPKL+DNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTV 300
           FKYVIAYTAH+PSYADS GWVLASDHP +LD EDL+ +I ERVRGELRYLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           INKTIRT MMNETHVFTEEDARF HG+GL  +A
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Carg10793 vs. ExPASy TrEMBL
Match: A0A1S3CAR3 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.1e-163
Identity = 281/333 (84.38%), Postives = 303/333 (90.99%), Query Frame = 0


Query: 1   MLSPLCSALPALSIPTSNSTAVPHV---SEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60
           M S  CSAL  L IP S+S  + H+    +WFEE+LEDDLKWSFAVNR+LHA TS+FQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIII 180
           LKHKSIEKVVMCDIDRDVVNFCRAHL ENQDAFQDEKLHIIFDDAK GLEG+ EKFD+II
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYE+ IKPKL+DNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTV 300
           FKYVIAYTAH+PSYADS GWVLASDHP +LD EDL+ +I ERVRGELRYLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 331
           INKTIRT MMNETHVFTEEDARF HG+GL  +A
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Carg10793 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 3.9e-118
Identity = 197/314 (62.74%), Postives = 249/314 (79.30%), Query Frame = 0


Query: 15  PTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLDGK 74
           PT    +      W+EE ++DDLKWSFA+N +LH  TS++QDI LLDTKRFGK L++DGK
Sbjct: 22  PTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGK 81

Query: 75  LQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDIDRD 134
           +QSAE+DEFIYHE ++HPALL H NPKTVFI+GGGEGS ARE LKH +IEKVVMCDID++
Sbjct: 82  MQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQE 141

Query: 135 VVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDLADPHEGGPCNHLYT 194
           VV+FCR  L+ N DAF ++KL ++  DAK  LE + EKFDII+ DLADP EGGPC  LYT
Sbjct: 142 VVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYT 201

Query: 195 KSFYEEGIKPKLNDNGIFVTQAGPSGILSY-NVFSSIYNTVKHVFKYVIAYTAHIPSYAD 254
           KSFY+  +KPKL+ NGIFVTQAGP+GI ++  VF+SIYNT+K VFKYV AYTAH+PS+AD
Sbjct: 202 KSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFAD 261

Query: 255 SSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVF 314
           + GWV+ASDH F ++V+++D+RI ERV GEL YL+    VS+  +NKTI  A+  ET V+
Sbjct: 262 TWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVY 321

Query: 315 TEEDARFTHGQGLA 328
           +EE+ARF HG G+A
Sbjct: 322 SEENARFIHGHGVA 335

[Close]

BLAST of Carg10793 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 3.9e-118
Identity = 197/314 (62.74%), Postives = 249/314 (79.30%), Query Frame = 0


Query: 15  PTSNSTAVPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLDGK 74
           PT    +      W+EE ++DDLKWSFA+N +LH  TS++QDI LLDTKRFGK L++DGK
Sbjct: 15  PTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGK 74

Query: 75  LQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDIDRD 134
           +QSAE+DEFIYHE ++HPALL H NPKTVFI+GGGEGS ARE LKH +IEKVVMCDID++
Sbjct: 75  MQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQE 134

Query: 135 VVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIIISDLADPHEGGPCNHLYT 194
           VV+FCR  L+ N DAF ++KL ++  DAK  LE + EKFDII+ DLADP EGGPC  LYT
Sbjct: 135 VVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYT 194

Query: 195 KSFYEEGIKPKLNDNGIFVTQAGPSGILSY-NVFSSIYNTVKHVFKYVIAYTAHIPSYAD 254
           KSFY+  +KPKL+ NGIFVTQAGP+GI ++  VF+SIYNT+K VFKYV AYTAH+PS+AD
Sbjct: 195 KSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFAD 254

Query: 255 SSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTVINKTIRTAMMNETHVF 314
           + GWV+ASDH F ++V+++D+RI ERV GEL YL+    VS+  +NKTI  A+  ET V+
Sbjct: 255 TWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVY 314

Query: 315 TEEDARFTHGQGLA 328
           +EE+ARF HG G+A
Sbjct: 315 SEENARFIHGHGVA 328

[Close]

BLAST of Carg10793 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 129.8 bits (325), Expect = 4.0e-30
Identity = 81/269 (30.11%), Postives = 135/269 (50.19%), Query Frame = 0


Query: 15  PTSNSTAVPHVSEWFEEQ--LEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLD 74
           P   ST +P    WF E   +      S  V ++L    S +QD+++  +  +GK L+LD
Sbjct: 81  PACFSTVIP---GWFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLD 140

Query: 75  GKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDID 134
           G +Q  E+DE  Y E + H  L    NPK V ++GGG+G   RE  +H SIE++ MC+ID
Sbjct: 141 GVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEID 200

Query: 135 RDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPE-KFDIIISDLADPHEGGPCNH 194
           + VV+  +    +    ++D +++++  D    L+   E  +D +I D +DP   GP   
Sbjct: 201 KMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKE 260

Query: 195 LYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKYVIAYT-AHIPS 254
           L+ K F+ + +   L   G+  TQA  S  L  ++   I +  + +FK  + Y    +P+
Sbjct: 261 LFEKPFF-QSVARALRPGGVVCTQA-ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPT 320

Query: 255 YADS-SGWVLAS----DHPFKLDVEDLDK 275
           Y     G++L S    D  FK  +  +D+
Sbjct: 321 YPSGVIGFMLCSTEGPDVDFKHPLNPIDE 342

[Close]

BLAST of Carg10793 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 129.4 bits (324), Expect = 5.3e-30
Identity = 77/251 (30.68%), Postives = 127/251 (50.60%), Query Frame = 0


Query: 15  PTSNSTAVPHVSEWFEEQ--LEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLD 74
           P   ST +P    WF E   +      S  V ++L    S +QD+++  +  +GK L+LD
Sbjct: 81  PACFSTVIP---GWFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLD 140

Query: 75  GKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDID 134
           G +Q  E+DE  Y E + H  L    NPK V ++GGG+G   RE  +H SIE++ MC+ID
Sbjct: 141 GVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEID 200

Query: 135 RDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPE-KFDIIISDLADPHEGGPCNH 194
           + VV+  +    +    ++D +++++  D    L+   E  +D +I D +DP   GP   
Sbjct: 201 KMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKE 260

Query: 195 LYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKYVIAYT-AHIPS 254
           L+ K F+ + +   L   G+  TQA  S  L  ++   I +  + +FK  + Y    +P+
Sbjct: 261 LFEKPFF-QSVARALRPGGVVCTQA-ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPT 320

Query: 255 YADSSG-WVLA 261
           Y    G W+ A
Sbjct: 321 YPRHWGHWIYA 324

[Close]

BLAST of Carg10793 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 128.6 bits (322), Expect = 9.0e-30
Identity = 78/259 (30.12%), Postives = 132/259 (50.97%), Query Frame = 0


Query: 15  PTSNSTAVPHVSEWFEE--QLEDDLKWSFAVNRILHAATSKFQDIVLLDTKRFGKALLLD 74
           P+  S+ +P    WF E   +      S  V +IL    S +QD+++  +  +GK L+LD
Sbjct: 41  PSCMSSIIP---GWFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLD 100

Query: 75  GKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARETLKHKSIEKVVMCDID 134
           G +Q  E+DE  Y E + H  L    NPK V ++GGG+G   RE  +H S+E++ +C+ID
Sbjct: 101 GVIQLTERDECAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEID 160

Query: 135 RDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPE-KFDIIISDLADPHEGGPCNH 194
           + VV+  + +       ++D ++++I  D    L+   E  +D +I D +DP   GP   
Sbjct: 161 KMVVDVAKQYFPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKE 220

Query: 195 LYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHVFKYVIAYT-AHIPS 254
           L+ K F+ E +   L   G+  TQA  S  L  ++   I +  + +FK  + Y    +P+
Sbjct: 221 LFEKPFF-ESVNRALRPGGVVCTQA-ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPT 280

Query: 255 YADS-SGWVLASDHPFKLD 269
           Y     G++L S    ++D
Sbjct: 281 YPSGVIGFMLCSSEGPQVD 292

The following BLAST results are available for this feature:

BLAST of Carg10793 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030499.1	1.8e-189	100.00	Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022946737.1	3.1e-189	99.39	thermospermine synthase ACAULIS5-like [Cucurbita moschata]	[more]
KAG6599521.1	4.4e-188	99.39	Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022999223.1	2.4e-186	98.18	thermospermine synthase ACAULIS5-like [Cucurbita maxima]	[more]
XP_023546321.1	4.1e-186	98.18	thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg10793 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	5.5e-117	62.74	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]
Q975S5	1.4e-56	41.94	Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ...	[more]
Q9YE02	1.0e-54	41.00	Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1...	[more]
Q72K55	6.7e-54	39.73	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ...	[more]
Q5SK28	6.7e-54	39.73	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS...	[more]

BLAST of Carg10793 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4J8	1.5e-189	99.39	thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450...	[more]
A0A6J1KJ05	1.2e-186	98.18	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149366...	[more]
A0A0A0LET5	5.3e-163	83.48	PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 S...	[more]
A0A5A7UZV1	9.1e-163	84.38	Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CAR3	9.1e-163	84.38	thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE...	[more]

BLAST of Carg10793 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	3.9e-118	62.74	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	3.9e-118	62.74	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G23820.1	4.0e-30	30.11	spermidine synthase 1	[more]
AT1G23820.2	5.3e-30	30.68	spermidine synthase 1	[more]
AT1G70310.1	9.0e-30	30.12	spermidine synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 28..79 e-value: 6.8E-13 score: 48.3
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 79..329 e-value: 5.2E-73 score: 247.6
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 82..254 e-value: 1.6E-37 score: 128.7
None	No IPR available	PANTHER	PTHR43317:SF6	THERMOSPERMINE SYNTHASE ACAULIS5-LIKE	coord: 22..327
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 22..327
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 102..215 e-value: 5.21508E-7 score: 45.4987
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 26..78 e-value: 1.1E-16 score: 62.6
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 26..262 score: 87.754395
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 27..308 score: 30.126999
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 26..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10793-RA	Carg10793-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0010487	thermospermine synthase activity
molecular_function	GO:0003824	catalytic activity