Carg10759 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10759
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Eukaryotic translation initiation factor 5A
Location	Carg_Chr01: 11589971 .. 11592268 (+)
RNA-Seq Expression	Carg10759
Synteny	Carg10759

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTGTCCGGATAAAACAGATGATATAAATTGCTCTCGTCCTCTTGACTCGTATTCTCCTCCTTCCTCCCGCTTTCCTTCCGTTTCCACCGAAATCCCACTGTACAACACACAAGAAAAAGCTCGGAAGCTGTAGAAGTGCGAAATCTTCAGCCATGTCGGACGAGGAGCACCACTTTGAATCCAAGGCCGACGCCGGAGCCTCCAAAACCTACCCTCAACAGGCTGGAACCATCCGGAAGAACGGCTACATCGTCATCAAGAACCGTCCATGCAAGGTGATTTGTTGTTTTTGTGTAATGATTTGTTGTGTTTGTTGCGATGATTTGGTTTTCTCGTTGTTTCGATCGTCCAGTGTAATCTTTTGTGGTGTTTAGGGTTGGGTTTGGGTCGGGCGCTTTAGATCTTTGGTTGATTTGAAAATTTTATTTCGCTTGATAATTGATTGTTATAGATTATGTGCGATTTATCGTATATTACATAGCGTAGACGGTGAGAATTGTCCCAAGTGTTGGATTTGGGAAAATGGATAAATCTGAAATTACCATTATTGATCATATGGATCAATATTTGTTGAGAGATCCGAGGGTTGTAATTATGATTTCACAGAGTTTCTTGATGGTTTAGTATTGTTCGTTCAACGAATAACAGTGATTTTTCTCCTCATAACTCTGAGAGCACGCTGGTGTTTACACTGCAGTTGTTATTGATTCCCGGAGATTAATTTCCTCAGTGTCATATTTCCCATAATTGATGTTTCCTTGTTCTTAAGCGCGAGGGTGCGTTTGTATTTATTGAGACTGTTATTGCTACCTGATAATAATTAGTGAAAAAGTCTATCCGAAGCTCCTAGAAGGCTAATTGAATAGTGCTTCCAAATTATACTTTTAGGCATTAAAGTAGAATTGCCTCTAGCCTTCCTTTTATTTGTAAGGCTACTTTCTATGCTACGTCTCTGTGTTTAAATCTCAGTATGTTGGTTTTTTTCCCTCTGGTTCGTTTGGTAATGCAGGTGGTTGAGGTTTCAACTTCTAAGACTGGCAAGCACGGGCATGCTAAGTGTCACTTCGTCGGAATTGACATCTTCACTGCCAAGAAGCTCGAAGATATTGTTCCATCATCCCACAATTGTGATGTAAGGAGTGTATTATATAGCCATTTAACCGGTTTATATCCGTTCCACTAATGGTTCTTATTTCATGCTTTGTAGGTGCCTCATGTTAATCGTACTGATTATCAGCTGATTGATATCTCTGAGGATGGATTTGTGAGTAATTTGGTTACATGAGATTGTTTGATTTAAATTTGTCTTCCCGACTTCAAGAAACTTAGTTATTTCTGATGTTAAATATATTTGTCTATATGTATATAGTTCAATGTGTTTTTATAATTCATTATAGAGGTGAATCTCCCTGGCTTGGTGTCTCTACTTCAATTCATAGATAAGCAACCGTAGATAGAGGCAATGAATCATTCATACATGCTGCAGCTTGTCGCGTAAAGCCTTTTATCTCGATTCATGTTGTTTTCTTTGCTGCAGGTGAGTCTTTTGACTGACAATGGTAACACCAAGGATGATTTGAGGCTCCCAACTGATGATAACCTGCTCTCTCAGGTAAAGTCACATATGAAACCTTATTATGGCTAGACGGATTCTCTGTCCAAAATTGAAGTTATTCGGGTAGATCTCATACCCTAATTTAGTTATTCGAGTAGCTCATTTCCATTTTCTGTTAGAAGGTACCTGATTCCTTCTGTTCACCCTGTTCCCCCTGTTCCCCGTATAATATATTCTCTGTCCTTGGTATATTTTTAAGATATGGTCATGTGGGTTTATTCTTAATATTATTCCATTTTTGTGTTGATGTCTATTTAATACGTACGAACTGAATCCGTTGTTTGTATCCACAGATTAAGGATGGATTTGCTGAAGGAAAGGACCTTGTCGTGTCGGTGATGTCTGCCATGGGAGAGGAACAAATTTGTGCCCTGAAGGATATCGGGCCGAAGAACTAGCTTTGCTTCAAACAAAGTGAAGTTGTGGTCATCAACTGCTTTAGTAGCTGAATCATCCCTCGATTGATGAATCTTTGAACGATGATAAAATTAATGACTTTGTATCTGTTAACGCTTCAATCTTCACCTTCTTCTGGCCAAGGTTGTAAGATTGGTTCGTGCATCATTACTGTGTTTTATGTTGTGTTATTTTATGATATTGGGTGTAATGTTATTGGGTTTTTGGTTGCTTATTTTGATAATCCAAGTCAAATAATTTTTGCGAATGCACTTAGCTCTATAAT

mRNA sequence

CGTGTCCGGATAAAACAGATGATATAAATTGCTCTCGTCCTCTTGACTCGTATTCTCCTCCTTCCTCCCGCTTTCCTTCCGTTTCCACCGAAATCCCACTGTACAACACACAAGAAAAAGCTCGGAAGCTGTAGAAGTGCGAAATCTTCAGCCATGTCGGACGAGGAGCACCACTTTGAATCCAAGGCCGACGCCGGAGCCTCCAAAACCTACCCTCAACAGGCTGGAACCATCCGGAAGAACGGCTACATCGTCATCAAGAACCGTCCATGCAAGGTGGTTGAGGTTTCAACTTCTAAGACTGGCAAGCACGGGCATGCTAAGTGTCACTTCGTCGGAATTGACATCTTCACTGCCAAGAAGCTCGAAGATATTGTTCCATCATCCCACAATTGTGATGTGCCTCATGTTAATCGTACTGATTATCAGCTGATTGATATCTCTGAGGATGGATTTGTGAGTCTTTTGACTGACAATGGTAACACCAAGGATGATTTGAGGCTCCCAACTGATGATAACCTGCTCTCTCAGATTAAGGATGGATTTGCTGAAGGAAAGGACCTTGTCGTGTCGGTGATGTCTGCCATGGGAGAGGAACAAATTTGTGCCCTGAAGGATATCGGGCCGAAGAACTAGCTTTGCTTCAAACAAAGTGAAGTTGTGGTCATCAACTGCTTTAGTAGCTGAATCATCCCTCGATTGATGAATCTTTGAACGATGATAAAATTAATGACTTTGTATCTGTTAACGCTTCAATCTTCACCTTCTTCTGGCCAAGGTTGTAAGATTGGTTCGTGCATCATTACTGTGTTTTATGTTGTGTAATGTTATTGGGTTTTTGGTTGCTTATTTTGATAATCCAAGTCAAATAATTTTTGCGAATGCACTTAGCTCTATAAT

Coding sequence (CDS)

ATGTCGGACGAGGAGCACCACTTTGAATCCAAGGCCGACGCCGGAGCCTCCAAAACCTACCCTCAACAGGCTGGAACCATCCGGAAGAACGGCTACATCGTCATCAAGAACCGTCCATGCAAGGTGGTTGAGGTTTCAACTTCTAAGACTGGCAAGCACGGGCATGCTAAGTGTCACTTCGTCGGAATTGACATCTTCACTGCCAAGAAGCTCGAAGATATTGTTCCATCATCCCACAATTGTGATGTGCCTCATGTTAATCGTACTGATTATCAGCTGATTGATATCTCTGAGGATGGATTTGTGAGTCTTTTGACTGACAATGGTAACACCAAGGATGATTTGAGGCTCCCAACTGATGATAACCTGCTCTCTCAGATTAAGGATGGATTTGCTGAAGGAAAGGACCTTGTCGTGTCGGTGATGTCTGCCATGGGAGAGGAACAAATTTGTGCCCTGAAGGATATCGGGCCGAAGAACTAG

Protein sequence

MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTDDNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN

Homology

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BLAST of Carg10759 vs. NCBI nr
Match: KAG6591533.1 (hypothetical protein SDJN03_13879, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 328.9 bits (842), Expect = 2.4e-86
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 101 MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 160

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 161 VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 220

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 221 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 260

[Close]

BLAST of Carg10759 vs. NCBI nr
Match: XP_022936947.1 (eukaryotic translation initiation factor 5A-2 [Cucurbita moschata] >XP_022941214.1 eukaryotic translation initiation factor 5A-2 [Cucurbita moschata] >XP_022976265.1 eukaryotic translation initiation factor 5A-2 [Cucurbita maxima] >XP_022981963.1 eukaryotic translation initiation factor 5A-2 [Cucurbita maxima] >XP_023524407.1 eukaryotic translation initiation factor 5A-2 [Cucurbita pepo subsp. pepo] >KAG6608302.1 hypothetical protein SDJN03_01644, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024419.1 hypothetical protein SDJN02_13234 [Cucurbita argyrosperma subsp. argyrosperma] >KAG7037649.1 hypothetical protein SDJN02_01279 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 328.9 bits (842), Expect = 2.4e-86
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of Carg10759 vs. NCBI nr
Match: XP_023535726.1 (eukaryotic translation initiation factor 5A-4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 327.0 bits (837), Expect = 9.0e-86
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           D+LLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DSLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of Carg10759 vs. NCBI nr
Match: XP_022133256.1 (eukaryotic translation initiation factor 5A-2 [Momordica charantia])

HSP 1 Score: 324.3 bits (830), Expect = 5.8e-85
Identity = 157/160 (98.12%), Postives = 159/160 (99.38%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNG+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFT KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLL+QIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of Carg10759 vs. NCBI nr
Match: PON41654.1 (Translation elongation factor [Trema orientale])

HSP 1 Score: 324.3 bits (830), Expect = 5.8e-85
Identity = 157/160 (98.12%), Postives = 158/160 (98.75%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIF  KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLL+QIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

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BLAST of Carg10759 vs. ExPASy Swiss-Prot
Match: Q9AXJ4 (Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV=2)

HSP 1 Score: 314.7 bits (805), Expect = 6.0e-85
Identity = 151/160 (94.38%), Postives = 157/160 (98.12%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKT+PQQAGTIRKNGYIVIKNRPCKV+EVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVMEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIF  KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+ GNTKDDLRLPTD
Sbjct: 61  VGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTETGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           +NLLSQIKDGFAEGKDLVVSVMSAMGEE+IC+LKDIGPKN
Sbjct: 121 ENLLSQIKDGFAEGKDLVVSVMSAMGEERICSLKDIGPKN 160

[Close]

BLAST of Carg10759 vs. ExPASy Swiss-Prot
Match: P24922 (Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=4092 GN=EIF-5A2 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 2.3e-84
Identity = 152/159 (95.60%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEH FESKADAGASKTYPQQAGTIRKNG+IVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGTIRKNGHIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NGNTKDDLRLPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 160
           DNLL+QIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg10759 vs. ExPASy Swiss-Prot
Match: P69039 (Eukaryotic translation initiation factor 5A-1 OS=Nicotiana plumbaginifolia OX=4092 GN=EIF-5A1 PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 5.1e-84
Identity = 149/159 (93.71%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNG+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NGNTKDDLRLPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 160
           DNLL+ IKDGFAEGKDLV+SVMSAMGEEQIC +KD+GPK
Sbjct: 121 DNLLALIKDGFAEGKDLVLSVMSAMGEEQICGIKDVGPK 159

[Close]

BLAST of Carg10759 vs. ExPASy Swiss-Prot
Match: P69040 (Eukaryotic translation initiation factor 5A-1 OS=Nicotiana tabacum OX=4097 GN=EIF-5A1 PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 5.1e-84
Identity = 149/159 (93.71%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNG+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NGNTKDDLRLPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 160
           DNLL+ IKDGFAEGKDLV+SVMSAMGEEQIC +KD+GPK
Sbjct: 121 DNLLALIKDGFAEGKDLVLSVMSAMGEEQICGIKDVGPK 159

[Close]

BLAST of Carg10759 vs. ExPASy Swiss-Prot
Match: P56337 (Eukaryotic translation initiation factor 5A-5 OS=Solanum tuberosum OX=4113 GN=EIF5A5 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.5e-83
Identity = 148/159 (93.08%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRK+G+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKSGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFT KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NGNTKDDLRLPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 160
           D LL+Q+KDGFAEGKDLV+SVMSAMGEEQIC +KDIGPK
Sbjct: 121 DTLLAQVKDGFAEGKDLVLSVMSAMGEEQICGIKDIGPK 159

[Close]

BLAST of Carg10759 vs. ExPASy TrEMBL
Match: A0A6J1IIZ4 (Eukaryotic translation initiation factor 5A OS=Cucurbita maxima OX=3661 GN=LOC111476711 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.1e-86
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of Carg10759 vs. ExPASy TrEMBL
Match: A0A6J1FMM9 (Eukaryotic translation initiation factor 5A OS=Cucurbita moschata OX=3662 GN=LOC111446587 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.1e-86
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of Carg10759 vs. ExPASy TrEMBL
Match: A0A6J1BUI3 (Eukaryotic translation initiation factor 5A OS=Momordica charantia OX=3673 GN=LOC111005893 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.8e-85
Identity = 157/160 (98.12%), Postives = 159/160 (99.38%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNG+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFT KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLL+QIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of Carg10759 vs. ExPASy TrEMBL
Match: A0A2P5AYM9 (Eukaryotic translation initiation factor 5A OS=Trema orientale OX=63057 GN=TorRG33x02_337690 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.8e-85
Identity = 157/160 (98.12%), Postives = 158/160 (98.75%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIF  KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD
Sbjct: 61  VGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 161
           DNLL+QIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of Carg10759 vs. ExPASy TrEMBL
Match: A0A2P6R2R8 (Eukaryotic translation initiation factor 5A OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0439011 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 3.7e-85
Identity = 157/159 (98.74%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NGNTKDDLRLPTD
Sbjct: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 160
           DNLL+QIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg10759 vs. TAIR 10
Match: AT1G13950.1 (eukaryotic elongation factor 5A-1 )

HSP 1 Score: 295.0 bits (754), Expect = 3.5e-80
Identity = 145/159 (91.19%), Postives = 151/159 (94.97%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFES +DAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFES-SDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFT+KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDG+VSLLTDNG+TKDDL+LP D
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPND 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 160
           D LL QIK GF +GKDLVVSVMSAMGEEQI ALKDIGPK
Sbjct: 121 DTLLQQIKSGFDDGKDLVVSVMSAMGEEQINALKDIGPK 158

[Close]

BLAST of Carg10759 vs. TAIR 10
Match: AT1G69410.1 (eukaryotic elongation factor 5A-3 )

HSP 1 Score: 286.6 bits (732), Expect = 1.3e-77
Identity = 137/159 (86.16%), Postives = 151/159 (94.97%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHFES +DAGASKTYPQQAG IRK G+IVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFES-SDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFT+KKLEDIVPSSHNCDVPHVNR DYQLIDISEDGFVSLLTDNG+TKDDL+LPTD
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 160
           + LL+Q+K+GF EGKD+VVSVMSAMGEEQ+CALK++GPK
Sbjct: 121 EALLTQLKNGFEEGKDIVVSVMSAMGEEQMCALKEVGPK 158

[Close]

BLAST of Carg10759 vs. TAIR 10
Match: AT1G26630.1 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 271.6 bits (693), Expect = 4.2e-73
Identity = 129/157 (82.17%), Postives = 146/157 (92.99%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHFE+ +++GASKTYPQ AG IRK G+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFTAKKLEDIVPSSHNCDVPHVNR DYQLIDI+EDGFVSLLTD+G TKDDL+LPTD
Sbjct: 61  VAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTD 120

Query: 121 DNLLSQIKDGFAEGKDLVVSVMSAMGEEQICALKDIG 158
           D L +Q++ GF EGKD+VVSVMS+MGEEQICA+K++G
Sbjct: 121 DGLTAQMRLGFDEGKDIVVSVMSSMGEEQICAVKEVG 156

[Close]

BLAST of Carg10759 vs. TAIR 10
Match: AT1G26630.2 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 226.5 bits (576), Expect = 1.5e-59
Identity = 107/127 (84.25%), Postives = 118/127 (92.91%), Query Frame = 0


Query: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHFE+ +++GASKTYPQ AG IRK G+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VGIDIFTAKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTD 120
           V IDIFTAKKLEDIVPSSHNCDVPHVNR DYQLIDI+EDGFVSLLTD+G TKDDL+LPTD
Sbjct: 61  VAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTD 120

Query: 121 DNLLSQI 128
           D L +Q+
Sbjct: 121 DGLTAQL 126

The following BLAST results are available for this feature:

BLAST of Carg10759 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6591533.1	2.4e-86	100.00	hypothetical protein SDJN03_13879, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022936947.1	2.4e-86	100.00	eukaryotic translation initiation factor 5A-2 [Cucurbita moschata] >XP_022941214...	[more]
XP_023535726.1	9.0e-86	99.38	eukaryotic translation initiation factor 5A-4 [Cucurbita pepo subsp. pepo]	[more]
XP_022133256.1	5.8e-85	98.13	eukaryotic translation initiation factor 5A-2 [Momordica charantia]	[more]
PON41654.1	5.8e-85	98.13	Translation elongation factor [Trema orientale]	[more]

BLAST of Carg10759 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9AXJ4	6.0e-85	94.38	Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV...	[more]
P24922	2.3e-84	95.60	Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=40...	[more]
P69039	5.1e-84	93.71	Eukaryotic translation initiation factor 5A-1 OS=Nicotiana plumbaginifolia OX=40...	[more]
P69040	5.1e-84	93.71	Eukaryotic translation initiation factor 5A-1 OS=Nicotiana tabacum OX=4097 GN=EI...	[more]
P56337	1.5e-83	93.08	Eukaryotic translation initiation factor 5A-5 OS=Solanum tuberosum OX=4113 GN=EI...	[more]

BLAST of Carg10759 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IIZ4	1.1e-86	100.00	Eukaryotic translation initiation factor 5A OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1FMM9	1.1e-86	100.00	Eukaryotic translation initiation factor 5A OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1BUI3	2.8e-85	98.13	Eukaryotic translation initiation factor 5A OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A2P5AYM9	2.8e-85	98.13	Eukaryotic translation initiation factor 5A OS=Trema orientale OX=63057 GN=TorRG...	[more]
A0A2P6R2R8	3.7e-85	98.74	Eukaryotic translation initiation factor 5A OS=Rosa chinensis OX=74649 GN=RchiOB...	[more]

BLAST of Carg10759 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13950.1	3.5e-80	91.19	eukaryotic elongation factor 5A-1	[more]
AT1G69410.1	1.3e-77	86.16	eukaryotic elongation factor 5A-3	[more]
AT1G26630.1	4.2e-73	82.17	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]
AT1G26630.2	1.5e-59	84.25	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020189	Translation elongation factor, IF5A C-terminal	SMART	SM01376	eIF_5a_2	coord: 85..154 e-value: 1.7E-34 score: 130.6
IPR020189	Translation elongation factor, IF5A C-terminal	PFAM	PF01287	eIF-5a	coord: 85..154 e-value: 1.1E-29 score: 102.4
IPR014722	Ribosomal protein L2, domain 2	GENE3D	2.30.30.30		coord: 1..85 e-value: 3.7E-43 score: 147.3
IPR001884	Translation elongation factor IF5A-like	TIGRFAM	TIGR00037	TIGR00037	coord: 17..153 e-value: 2.5E-41 score: 138.5
IPR001884	Translation elongation factor IF5A-like	PIRSF	PIRSF003025	Transl_init_eIF5A	coord: 1..157 e-value: 3.9E-67 score: 222.6
IPR001884	Translation elongation factor IF5A-like	PANTHER	PTHR11673	TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER	coord: 1..156
None	No IPR available	GENE3D	2.40.50.140		coord: 86..159 e-value: 6.7E-35 score: 121.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11673:SF42	BNAA07G09420D PROTEIN	coord: 1..156
None	No IPR available	CDD	cd04468	S1_eIF5A	coord: 86..156 e-value: 9.53766E-32 score: 106.468
IPR019769	Translation elongation factor, IF5A, hypusine site	PROSITE	PS00302	IF5A_HYPUSINE	coord: 50..57
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 86..156
IPR008991	Translation protein SH3-like domain superfamily	SUPERFAMILY	50104	Translation proteins SH3-like domain	coord: 15..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10759-RA	Carg10759-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045901	positive regulation of translational elongation
biological_process	GO:0045905	positive regulation of translational termination
biological_process	GO:0006413	translational initiation
molecular_function	GO:0043022	ribosome binding
molecular_function	GO:0003746	translation elongation factor activity
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0003723	RNA binding