Carg10725 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10725
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionguanine nucleotide-binding protein alpha-1 subunit
LocationCarg_Chr01: 11765437 .. 11771732 (+)
RNA-Seq ExpressionCarg10725
SyntenyCarg10725
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTCCTTTTTTTATTCCTTCAATCAATTTAATATGATTATTCATCAAACCCCACTTCTGTTTTCACTCTACAACACCACCACCACTTCATTCACTGCTCTACGAATCCGCCAAATCTAGGCTACCCTTTTAAACCTTCTTCCAGATCTTCACTCAACTGGGATTTCATCTGCCTGTTCTTCACTTTCATCCAACACTCTCTTTTAGGTACTCATTACCCAATTCTTTACTGTTTTCTTTGATGGGTCTGCTCTGGATTTCATTTTATGAACTGTTGCTTTGAATTGGGTGTTTGGGTGTTGTTTTTTGCATGCTTGAAATGGGGTTTCAAGATTGTAATATGAGTGAGTGTTTTCAAAGGCGATCGCTGACCCATTTGATGCGGATGAAGCTGTGTGAGTGCTACCATTAGTGGTCTGTTGTTTTCTATGCTGTCTCATTTGAGTAGAAGTATGGGGTTACTCTGCAGCAGAAATCGTCGTTACAATAAACAAGATACTGAAGAGAATGTGCAGGTGAATCATGCTTTATTAGAATCTTTGATTCTTTGGTTTTAATGGTGGTTGAAGTAGCAATGTTGTGTTATTTGTTGAATCTCGGGTTCGATATGATCGTAAGTTGTGTGGTTTTCCAGGCTGCTGAAATAGAGAGGCGGATTGTTCAAGAAACAGAGGCTGAAAAACATATTCAGAAACTTCTTCTGCTTGGTAAGTGTATTTTAGCCTCACATTACCTTTGGGGGATTCCCCACCAGGGAACGAAACATTCCTTATAAGAGTACGTTAGTAAATGCGTTCTAAAACCTTGAGAGAAAGCCTAAAGAGGACAATATCTGTTGATGGTGGGCTTAGGCCGTTACAAATGGTACTAGAGCTGGGAACAGTGTGTCAGTGAGGACGCTGGCCCCCAAGTGGGTGGATTGTGAGATCTCACGTTCGTTGGAGAGAGGAACGAAACGTTCTTATTAGGGTGTGGAAACCTTTCCCTAGTAGACGCGTTTTAAAAGCCCAAAGAGGATATTAACAGCCCAAGCCCACCGCCAACCGATATTGTCCTCTTTGGGCTTTCTCTTTCGGGCTTCCCCTCAAGGTTTTTAAAATGCGTCTGATTGTGGTAGGGAGAGGTTTCCACACCTTTATAAAGAATGCTTTGTTTCCCTCTCGAATCAATGTGTGGTCTCACAATCCAACCCTTCGGGGCTCAACCTCCTTGCTGGCACATCACCCGGTGTCTGGCTCTGATACCATTTGTAACAACCCAATCCCACCGCTAGTAGATATTCTCTTCTTTAGGGGAGAGGTTTCCACACCCTTATAAAGAATACTTCCTTCTCCTTACCAACCGACGTGGGATCTCACAAAAGGATTGCAAATTTTCTTTTACAATGCAATGCAAATATGCTGAATTTACCCAATTGTTTAGGTGTCGGAGAGTCCGGGAAATCCACCATTTTTAAGCAGGCAAGCCCTTGTATTCTTTACCGTTATAATCGGCAACGTCAACGTCGTAATGTAGTCGGTCCACTAAACGTTTTTTGCTTTTGTCGCTTCATTTTTGCAGATAAAATTGTTGTTCCAAACTGGGTTTGATGAGGCAGAGCTGAAGAGCTATATTCCAGTCATTCATGCAAATGTGTATCAGACTATAAAAGTATGCAATATTTGAAAGGGTGTGTTGGTTATTTCCCTTTCTGCATGATTCAAACAGCAAATGATTTTGTCAAAAAATTTACAACATAATATCCCTTTTTATTTCGAGTGCCAAAACTCTAAATCATGGGTCGATATTGTATTGTTTGTCGATAACTATAACCTCTTATTCAACTCGACGTTTCAGGTATTACACGATGGTTCAAAGGAGCTCGCTCAAAGTGATAAAGAGTTCTCGAAGTATGTTTTGTCCAGTGAAAATAAGGTATCCGTTTTATGTCTAACTGGAGCTTGCATGACTCGAGCATTTGTCTCGTACCGTTTTATAATAATTGCCAGTGGTTGCATAAAGGATTTGTCCAGCTCGCTTTCTGGTTCATGATTCAGAAATAGAAACGGGGGCAGATTTATACGCAAAATGCGATTTATCTGAAATGTTGAATAACAAATGAAGGAGAATAAGAATTTACCCGAACCTAGCCACGAAATCAAGTAGGTAAGAAAGCCAATACTCACGAACTTAGGAAAGAAAATTGCTCGAACCCAAGTGAAAAGTATATCAAACACGACTAGAAGAAAGAAGAAAAAATTCCCACAAAAATAGCCTCGTTCATCGAAAAGACTACTTATAATCTAGCCACTGTTCTTCAATGTAAAATGGAAACTCGATTAAGTTGAAGGGTTTAATTTGATGAAATTGACGGTTGGCTAAATATGTCAGTTGCCTTGGATTGATGGTGTAACAATCCAAGCTCACCACTAGCAGATATTGTCCCTTTTAGCCTGTTATATATTGCCGTCAGCCTCACGGTTTTAAAACGCGTCTACTAGGAAGAGGCTTCCACACCCTTATAAGGAATGCTTTGTTCCCCTCTCCGACCGATGTGGGATCTCACAATCCACCCTCTTGAGGGCCCAACGTTCTCGCTGGTACATTACTTGATACCTAGCTTTGACACCATTTGTAGCAGCACAAGCCCACCGCTAGTAGATATTGTCCGTTTTTAGCCTGTTACGTATCGCCGTCAGCCTTACAATTTTAAAACGCGTCTACTAGGGAGAGGTTTCTACACCCTTATAAGGAATACGTCGTTTTCCTCTCCAACTGATGCGGGATCTCACAGGCCGAGTGTTGTACGGTAAAAGAAAAAGAAAAGGTTCAATGTTAAAGTTGGCTCTTTCAAATTTTGGCCCATGTATAGGGGTGAAAATGTATTTCTTCCCCTAAATATAATTTGCTGGACATGTACTGAACCTTTTGCTGCTTATTATGGCACCTAACTCGTGATTCTGCATCAGGACATTGGTGAGAAATTATCCGACATTGGCGGTAGATTGGATTACCCGCGTTTGACTAGGGAGCATGCACACGATATAGAGACTCTATGGAACGATGCTGCGATTCAGGTAGCCGTGGCCCCATGTAGATTAGCTAGTGGCATCTTTTAAATGAAATCGACCATGACGCTTCTTATTTTATGTAAGCTTTTTATCTTCTTGAATTCGTCCCAGATGAAGCGAGGTCAGCGTTCTTATTCTCTTTGTAGGAAACGTATGCTCGTGGTAACGAACTACAGGTTCCAGATTGTGCACATTATTTCATGGAAAATTTACAGAGATTATCTGATGTAAATTACATTCCAACTAAGGTATTCAATCTTAGTATCCTTTACAGTGAAACTAGTAACGAGATCGAGTTATTTGCGTAAATTTTGGGTCTTTTCTTATGTGGCTTTGATCACAGGAAGACGTGCTCTACGCAAGAGTTCGTACAACGGGTGTTGTCGAAATCCAGTTTAGGTAAATATTTAGTTACGTTTACAATGTGTTTTTATATTCCTTGTAATTTATGTATCTTAATTAAAATCTCGTAAGACGATTAAGCTCGTCTTACAGCTTAATCGTTATAATTAATTATGGTATTTGTTTGATTTTGGCAGTCCCGTTGGAGAAAATAAAAAGAGCGGAGAAGTGTATCGACTCTTTGATGTCGGTGGACAGAGAAACGAGAGGCGGAAATGGATTCATCTTTTTGAAGGTGTTACAGCAGTAATCTTTTGTGCTGCTATTAGTGAGTAAGACCATCTCTCTTTTAACCTATTTGTGATTCTGGTAGTCTTGACAAATGTAGAAGGAAAAAGATATATGCCCCTCTCATTCACGACCACCTGGTCGGGGATGCTCCCCGAAGCCCCGATGGGAAGCCATTTAAAGTTTTGTGAACGAGGGTCTTTTGCTAGTTTCTATTGTTTTCTTTTGAAGTCGGTGGGACTAGGTTGGAGAGTAGCTCTTAGACGATAGTTGTAACCGCCCAAGCCCACCGCTAGCCTATATTCACTTCTCTGCGCTTCTCTTCAAGATTTTAAAACGTGTCTATTAGGGAGAGATTTCCACACCCTTATAAGAAATGCTTTATTCTGCTCTCCAACCGATGTCGGATCTCACAATCCACCCCTAGGGACCAGCGAGGATGCTAGTGACACAATCGCGCTTTCGAAGGCAGCGGACTAGCGATTGTGCGGTACTCACTTTCCAGCCGACAAGTGAGCTTAAGCCAATTTGTTCTTGCGTCGCCTACGGCCAAGCGACGTACGATCGAGCCTCTAGCCTCGGTTTTAAAAGAAGGTTTTAGAAAATGAGGAGAGAGAGAGATTTTAGAAAAAGAGATTTTGGAAAGAGACGTTATATAAGCAAGCAAGAGAAAGATAACAACGGCTTAGCATATATAACCTTGGGTAGGGAGAAGTTGACAACTAGTCATATCCACCTCTAGTACATTCTGAGCCATTTTAAGTCTGGCAGGCCTAGACATGATATTTTTGAGGCGTCATACGACCTAGAATTACAAAAGTAAAAATACTAGAATGAAAAGACATGACAAGGGTTTGGCTTGTCATGGGCATGCGGCCATGGGCAACATGCCCTGGACGAGCATGACCGTGACAGCTAGCACACTGCGTAGTGTTTGGCTTTGATACCATGTGTAATAGCCAAATCCCACTGCTAGCAGATATTGTCGTTGGTGGGCTTTCCCCTCAAGGTTTTAAAACGTGTTTACTAGGGAGAGGTTTCCACACCCTTATAAGGAATGCTTCATTCTTCTCTCCGACTGATGTGGAATCTCACAATCCACCCCTCTTGAGGGCTCAGCGTCTTCGCTGACACACCGCTCGGTGTTTGGCTCCGATATAAAGCCCAAGCCCATTGCTATCAGATATTGTCCTCTTTGAGCTTTCCCTCGAGGTTTTAAAACGCGTGTACTAAGGAGGTTTCCACACCCTTGTAAGGAATGTTTCATTCTCCTCTCCAACCGATGTGAGATCTCACGTTAGCGGTTCAAGTTGAGTGTCCTAAGGAAATGAAGAGATAATTCTAAAAGTCTAAAATGATAGAGGAAAAGTAGATCATAGCTAGCTGGTGATCTAACAACCCATGTGGTGATCATGGTGGCAAGACATACTTGTTGTCTGTCGTCTTTTTTTGTCTGCATAGCTGTGCTAATCGATGTTCCACTCTTTGCATTATGCTTATAATCCACTCCGCTAGTATCGGGAGACGTGTTCGAGATTTTATATAAGAGACCGTAATGATTTAAACTATAATGAAAGTATAAACTTAGTTGTAGCTCCTTGTCTTTTTAACTCGAAAGAACAAGAATTGCATTAAATTATACTATTGCCATAAGGTTTATCAGATTCAAATGGGTTAACCGATAACGGTTTCAGGTATGATCAAACACTTTTCGAGGACGAACAGAAGAACCGAATGATGGAGACAAAGGAACTTTTTGAGTGGGTTCTGAAACAAGAGTGTTTTGAGGTTTACTTTTTTGCATCGTTTTCGTTTACGTGTTTACGCGAGTGTATTTGACGATAATTTCGTTCCACAGTTTGATGCTGTTTTATTCTGGGTGCAGAAAACGTCGTTCATGCTTTTTCTCAACAAGTTCGATATATTCGAGAAAAAGGTTCTACAAGTAAGTGTTGATAGAGAGTATTACGATGCGCTTCAATAGGTCCTTTAAATATTAATACGAATTAGCGTCTTATGATAGGTCCCTCTTAGTGTATGTGAATGGTTCAAAGATTACCAGCTGGTTTCGACTGGAAAACAAGAGATCGAGCATGCCTACGAGTACGTATCTACCCCTCCCCCCTCGTCCTCTATGCTTAAACCGACCCATATCCGTAGCGGGATGACTTGTAGTCTAGGGACAATGCTGCCGTTTTTTTCCCCTCTTTCTTGTAGTTGATCTAAAATAACACTTACTGATGCATACGAATCAATGAATTCAGGTTCGTGAAGAAAAAATTCGAGGAGTTGTATTTTCAGACGACGTCACCAGACCGAGTAGATCGGGTGTTTAAGGTATACAGAACGACTGCTCTCGATCAGAAACTCGTAAAGAAGACGTTCAAACTCGTAGACGAAACTTTGAGGCGGAGAAACCTGTTGGAGGCCGGGTTATTGTGACGTCGTGAACACAGCTGAAGAAGAAGGAAGTTGGAAGTCTGATTCATTGATTTAAAACATTTTTGTATCAAAGTTGAAATCTTTTTAACTATTTGTAATTTGATGATCCAAATTATTTTGAAATTACATAACATATGTTTGATTCATTGATTTAAAACATTTTTGTATCAAAGT

mRNA sequence

AATTCCTTTTTTTATTCCTTCAATCAATTTAATATGATTATTCATCAAACCCCACTTCTGTTTTCACTCTACAACACCACCACCACTTCATTCACTGCTCTACGAATCCGCCAAATCTAGGCTACCCTTTTAAACCTTCTTCCAGATCTTCACTCAACTGGGATTTCATCTGCCTGTTCTTCACTTTCATCCAACACTCTCTTTTAGGCGATCGCTGACCCATTTGATGCGGATGAAGCTGTGTGAGTGCTACCATTAGTGGTCTGTTGTTTTCTATGCTGTCTCATTTGAGTAGAAGTATGGGGTTACTCTGCAGCAGAAATCGTCGTTACAATAAACAAGATACTGAAGAGAATGTGCAGATAAAATTGTTGTTCCAAACTGGGTTTGATGAGGCAGAGCTGAAGAGCTATATTCCAGTCATTCATGCAAATGTGTATCAGACTATAAAAGTATTACACGATGGTTCAAAGGAGCTCGCTCAAAGTGATAAAGAGTTCTCGAAGTATGTTTTGTCCAGTGAAAATAAGGACATTGGTGAGAAATTATCCGACATTGGCGGTAGATTGGATTACCCGCGTTTGACTAGGGAGCATGCACACGATATAGAGACTCTATGGAACGATGCTGCGATTCAGGAAACGTATGCTCGTGGTAACGAACTACAGGTTCCAGATTGTGCACATTATTTCATGGAAAATTTACAGAGATTATCTGATGTAAATTACATTCCAACTAAGGAAGACGTGCTCTACGCAAGAGTTCGTACAACGGGTGTTGTCGAAATCCAGTTTAGTCCCGTTGGAGAAAATAAAAAGAGCGGAGAAGTGTATCGACTCTTTGATGTCGGTGGACAGAGAAACGAGAGGCGGAAATGGATTCATCTTTTTGAAGGTGTTACAGCAGTAATCTTTTGTGCTGCTATTAGTGAGTATGATCAAACACTTTTCGAGGACGAACAGAAGAACCGAATGATGGAGACAAAGGAACTTTTTGAGTGGGTTCTGAAACAAGAGTGTTTTGAGTTTGATGCTGTTTTATTCTGGGTGCAGAAAACGTCGTTCATGCTTTTTCTCAACAAGTTCGATATATTCGAGAAAAAGGTTCTACAAGTCCCTCTTAGTGTATGTGAATGGTTCAAAGATTACCAGCTGGTTTCGACTGGAAAACAAGAGATCGAGCATGCCTACGAGTTCGTGAAGAAAAAATTCGAGGAGTTGTATTTTCAGACGACGTCACCAGACCGAGTAGATCGGGTGTTTAAGGTATACAGAACGACTGCTCTCGATCAGAAACTCGTAAAGAAGACGTTCAAACTCGTAGACGAAACTTTGAGGCGGAGAAACCTGTTGGAGGCCGGGTTATTGTGACGTCGTGAACACAGCTGAAGAAGAAGGAAGTTGGAAGTCTGATTCATTGATTTAAAACATTTTTGTATCAAAGTTGAAATCTTTTTAACTATTTATTGATTTAAAACATTTTTGTATCAAAGT

Coding sequence (CDS)

ATGCTGTCTCATTTGAGTAGAAGTATGGGGTTACTCTGCAGCAGAAATCGTCGTTACAATAAACAAGATACTGAAGAGAATGTGCAGATAAAATTGTTGTTCCAAACTGGGTTTGATGAGGCAGAGCTGAAGAGCTATATTCCAGTCATTCATGCAAATGTGTATCAGACTATAAAAGTATTACACGATGGTTCAAAGGAGCTCGCTCAAAGTGATAAAGAGTTCTCGAAGTATGTTTTGTCCAGTGAAAATAAGGACATTGGTGAGAAATTATCCGACATTGGCGGTAGATTGGATTACCCGCGTTTGACTAGGGAGCATGCACACGATATAGAGACTCTATGGAACGATGCTGCGATTCAGGAAACGTATGCTCGTGGTAACGAACTACAGGTTCCAGATTGTGCACATTATTTCATGGAAAATTTACAGAGATTATCTGATGTAAATTACATTCCAACTAAGGAAGACGTGCTCTACGCAAGAGTTCGTACAACGGGTGTTGTCGAAATCCAGTTTAGTCCCGTTGGAGAAAATAAAAAGAGCGGAGAAGTGTATCGACTCTTTGATGTCGGTGGACAGAGAAACGAGAGGCGGAAATGGATTCATCTTTTTGAAGGTGTTACAGCAGTAATCTTTTGTGCTGCTATTAGTGAGTATGATCAAACACTTTTCGAGGACGAACAGAAGAACCGAATGATGGAGACAAAGGAACTTTTTGAGTGGGTTCTGAAACAAGAGTGTTTTGAGTTTGATGCTGTTTTATTCTGGGTGCAGAAAACGTCGTTCATGCTTTTTCTCAACAAGTTCGATATATTCGAGAAAAAGGTTCTACAAGTCCCTCTTAGTGTATGTGAATGGTTCAAAGATTACCAGCTGGTTTCGACTGGAAAACAAGAGATCGAGCATGCCTACGAGTTCGTGAAGAAAAAATTCGAGGAGTTGTATTTTCAGACGACGTCACCAGACCGAGTAGATCGGGTGTTTAAGGTATACAGAACGACTGCTCTCGATCAGAAACTCGTAAAGAAGACGTTCAAACTCGTAGACGAAACTTTGAGGCGGAGAAACCTGTTGGAGGCCGGGTTATTGTGA

Protein sequence

MLSHLSRSMGLLCSRNRRYNKQDTEENVQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENLQRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL
Homology
BLAST of Carg10725 vs. NCBI nr
Match: KAG7037682.1 (Guanine nucleotide-binding protein alpha-1 subunit [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 730.7 bits (1885), Expect = 6.1e-207
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENVQIKLLFQTGFDEAELKSYIPVIHANVYQTIKV 60
           MLSHLSRSMGLLCSRNRRYNKQDTEENVQIKLLFQTGFDEAELKSYIPVIHANVYQTIKV
Sbjct: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENVQIKLLFQTGFDEAELKSYIPVIHANVYQTIKV 60

Query: 61  LHDGSKELAQSDKEFSKYVLSSENKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAI 120
           LHDGSKELAQSDKEFSKYVLSSENKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAI
Sbjct: 61  LHDGSKELAQSDKEFSKYVLSSENKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAI 120

Query: 121 QETYARGNELQVPDCAHYFMENLQRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENK 180
           QETYARGNELQVPDCAHYFMENLQRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENK
Sbjct: 121 QETYARGNELQVPDCAHYFMENLQRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENK 180

Query: 181 KSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELF 240
           KSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELF
Sbjct: 181 KSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELF 240

Query: 241 EWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQE 300
           EWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQE
Sbjct: 241 EWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQE 300

Query: 301 IEHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLE 360
           IEHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLE
Sbjct: 301 IEHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLE 360

Query: 361 AGLL 365
           AGLL
Sbjct: 361 AGLL 364

BLAST of Carg10725 vs. NCBI nr
Match: XP_022941404.1 (guanine nucleotide-binding protein alpha-1 subunit [Cucurbita moschata] >XP_022941405.1 guanine nucleotide-binding protein alpha-1 subunit [Cucurbita moschata])

HSP 1 Score: 681.8 bits (1758), Expect = 3.3e-192
Identity = 352/401 (87.78%), Postives = 354/401 (88.28%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLSRSMGLLCSRNRRYNKQDTEENV                                
Sbjct: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENVQAAEIERRIVQETEAEKHIQKLLLLGVGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSS+
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSD 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL
Sbjct: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH
Sbjct: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVLQVPLSVCEWFKDYQ +STGKQEIEHAYEFVKKKFEELYFQTTSPD
Sbjct: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQPISTGKQEIEHAYEFVKKKFEELYFQTTSPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 392

BLAST of Carg10725 vs. NCBI nr
Match: XP_022982370.1 (guanine nucleotide-binding protein alpha-1 subunit [Cucurbita maxima] >XP_022982372.1 guanine nucleotide-binding protein alpha-1 subunit [Cucurbita maxima])

HSP 1 Score: 678.3 bits (1749), Expect = 3.6e-191
Identity = 350/401 (87.28%), Postives = 353/401 (88.03%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLSRSMGLLCSRNRRYNKQDTEENV                                
Sbjct: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENVQDSEIERRIVQETEAEKHIQKLLLLGAGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQ+DKEFSKYVLSSE
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQNDKEFSKYVLSSE 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NKDIGEKLSDIG RLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL
Sbjct: 121 NKDIGEKLSDIGSRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQ+NERRKWIH
Sbjct: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQKNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVLQVPLSVCEWFKDYQ VSTGKQEIEHAYEFVKKKFEELYFQTTSPD
Sbjct: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKL+KKTFKLVDETLRRRNLLEAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLIKKTFKLVDETLRRRNLLEAGLL 392

BLAST of Carg10725 vs. NCBI nr
Match: XP_023524236.1 (guanine nucleotide-binding protein alpha-1 subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.9 bits (1748), Expect = 4.7e-191
Identity = 351/401 (87.53%), Postives = 353/401 (88.03%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLSRSMGLLCSRNRRYNKQDTEENV                                
Sbjct: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENVQAAEIERRIVQETEAEKHIQKLLLLGAGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQ+D EFSKYVLSSE
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQNDIEFSKYVLSSE 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL
Sbjct: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH
Sbjct: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVLQVPLSVCEWF+DYQ VSTGKQEIEHAYEFVKKKFEELYFQTTSPD
Sbjct: 301 MLFLNKFDIFEKKVLQVPLSVCEWFRDYQPVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 392

BLAST of Carg10725 vs. NCBI nr
Match: KAG6608333.1 (Guanine nucleotide-binding protein alpha-1 subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 651.4 bits (1679), Expect = 4.7e-183
Identity = 333/368 (90.49%), Postives = 341/368 (92.66%), Query Frame = 0

Query: 9   MGLLCSRNRRYNKQDTEENVQI-----KLLFQTGFDE-------AELKSYIPVIHANVYQ 68
           MGLLCSRNRRYNKQDTEENVQ      +++ +T  ++        ELKSYIPVIHANVYQ
Sbjct: 1   MGLLCSRNRRYNKQDTEENVQAAEIERRIVQETEAEKHIQKLLLLELKSYIPVIHANVYQ 60

Query: 69  TIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKLSDIGGRLDYPRLTREHAHDIETLWN 128
           TIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKLSDIGGRLDYPRLTREHAHDIETLWN
Sbjct: 61  TIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKLSDIGGRLDYPRLTREHAHDIETLWN 120

Query: 129 DAAIQETYARGNELQVPDCAHYFMENLQRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPV 188
           DAAIQETYARGNELQVPDCAHYFMENLQRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPV
Sbjct: 121 DAAIQETYARGNELQVPDCAHYFMENLQRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPV 180

Query: 189 GENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAISEYDQTLFEDEQKNRMMET 248
           GENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAISEYDQTLFE+EQKNRMMET
Sbjct: 181 GENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAVIFCAAISEYDQTLFENEQKNRMMET 240

Query: 249 KELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVST 308
           KELFEWVLKQECFE         KTSFMLFLNKFDIFEKKVLQVPLSVCEWFKDYQ +ST
Sbjct: 241 KELFEWVLKQECFE---------KTSFMLFLNKFDIFEKKVLQVPLSVCEWFKDYQPIST 300

Query: 309 GKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTALDQKLVKKTFKLVDETLRRR 365
           GKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTALDQKLVKKTFKLVDETLRRR
Sbjct: 301 GKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTALDQKLVKKTFKLVDETLRRR 359

BLAST of Carg10725 vs. ExPASy Swiss-Prot
Match: P49082 (Guanine nucleotide-binding protein alpha-1 subunit OS=Lotus japonicus OX=34305 GN=GPA1 PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.1e-171
Identity = 308/393 (78.37%), Postives = 327/393 (83.21%), Query Frame = 0

Query: 9   MGLLCSRNRRYNKQDTEENV-------------------------------------QIK 68
           MGLLCS+NRRYN  DTEEN                                      QIK
Sbjct: 1   MGLLCSKNRRYNDADTEENTQTAEIERRIELETKAEKHIQKLLLLGAGESGKSTIFKQIK 60

Query: 69  LLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKL 128
           LLFQTGFDEAELKSY PVIHANVYQTIK+LHDG+KELAQ+D +FSKYV+S ENK+IGEKL
Sbjct: 61  LLFQTGFDEAELKSYQPVIHANVYQTIKLLHDGAKELAQNDVDFSKYVISDENKEIGEKL 120

Query: 129 SDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENLQRLSDVNY 188
           S+IGGRLDYP LT+E A +IE LW DAAIQETYARGNELQVPDC HYFMENL RLSD NY
Sbjct: 121 SEIGGRLDYPCLTKELALEIENLWKDAAIQETYARGNELQVPDCTHYFMENLHRLSDANY 180

Query: 189 IPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV 248
           +PTK+DVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGV+AV
Sbjct: 181 VPTKDDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVSAV 240

Query: 249 IFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFD 308
           IFCAAISEYDQTLFEDE KNRMMETKELFEWVLKQ CFE         KTSFMLFLNKFD
Sbjct: 241 IFCAAISEYDQTLFEDENKNRMMETKELFEWVLKQPCFE---------KTSFMLFLNKFD 300

Query: 309 IFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKV 365
           IFEKK+L+VPL+VCEWFKDYQ VSTGKQEIEHAYEFVKKKFEE YFQ T+PDRVDRVFK+
Sbjct: 301 IFEKKILKVPLNVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQNTAPDRVDRVFKI 360

BLAST of Carg10725 vs. ExPASy Swiss-Prot
Match: Q40224 (Guanine nucleotide-binding protein alpha-1 subunit OS=Lupinus luteus OX=3873 GN=GPA1 PE=2 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.3e-170
Identity = 306/393 (77.86%), Postives = 327/393 (83.21%), Query Frame = 0

Query: 9   MGLLCSRNRRYNKQDTEENV-------------------------------------QIK 68
           MGLLCSRNRRYN  D EEN                                      QIK
Sbjct: 1   MGLLCSRNRRYNDADAEENAQAAEIERRIELETKAEKHIQKLLLLGAGESGKSTIFKQIK 60

Query: 69  LLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKL 128
           LLFQTGFDEAELKSY+PVIHANV+QTIK+LHDGSKELAQ+D + SKYV+S ENKDIGEKL
Sbjct: 61  LLFQTGFDEAELKSYLPVIHANVFQTIKLLHDGSKELAQNDVDSSKYVISDENKDIGEKL 120

Query: 129 SDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENLQRLSDVNY 188
           S+IG +LDYP LT E A +IETLW DAAIQETYARGNELQVP CAHYFMENLQRLSD NY
Sbjct: 121 SEIGSKLDYPYLTTELAKEIETLWEDAAIQETYARGNELQVPGCAHYFMENLQRLSDANY 180

Query: 189 IPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV 248
           +PTKEDVLYARVRTTGVVEIQFSPVGENK+SGEVYRLFDVGGQRNERRKWIHLFEGV+AV
Sbjct: 181 VPTKEDVLYARVRTTGVVEIQFSPVGENKRSGEVYRLFDVGGQRNERRKWIHLFEGVSAV 240

Query: 249 IFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFD 308
           IFCAAISEYDQTLFEDE KNRM ETKELFEW+LKQ CFE         KTSFMLFLNKFD
Sbjct: 241 IFCAAISEYDQTLFEDENKNRMTETKELFEWILKQPCFE---------KTSFMLFLNKFD 300

Query: 309 IFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKV 365
           IFEKK+L+VPL+VCEWFKDYQ VSTGKQEIEHAYEFVKKKFEELYFQ+T+P+RVDRVFKV
Sbjct: 301 IFEKKILKVPLNVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEELYFQSTAPERVDRVFKV 360

BLAST of Carg10725 vs. ExPASy Swiss-Prot
Match: O04278 (Guanine nucleotide-binding protein alpha-1 subunit OS=Pisum sativum OX=3888 GN=GPA1 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.1e-170
Identity = 303/393 (77.10%), Postives = 326/393 (82.95%), Query Frame = 0

Query: 9   MGLLCSRNRRYNKQDTEENV-------------------------------------QIK 68
           MGLLCS++ RYN    EEN                                      QIK
Sbjct: 1   MGLLCSKSNRYNDAKAEENAQTAEIERRIELETKAEKHIRKLLLLGAGESGKSTIFKQIK 60

Query: 69  LLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKL 128
           LLFQTGFDEAELKSY+PV+HANVYQTIK+LHDGSKE AQ+D +FSKYV+S+ENKDIGEKL
Sbjct: 61  LLFQTGFDEAELKSYLPVVHANVYQTIKLLHDGSKEFAQNDVDFSKYVISTENKDIGEKL 120

Query: 129 SDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENLQRLSDVNY 188
           S+IGGRLDYPRLT+E A +IE++W DAAIQETYARGNELQVPDC HYFMENLQRLSD NY
Sbjct: 121 SEIGGRLDYPRLTKELAQEIESIWKDAAIQETYARGNELQVPDCTHYFMENLQRLSDANY 180

Query: 189 IPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV 248
           +PTKEDVL ARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGV+AV
Sbjct: 181 VPTKEDVLLARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVSAV 240

Query: 249 IFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFD 308
           IFC AISEYDQTLFEDE KNRMMETKELFEWVLKQ+CFE         KTSFMLFLNKFD
Sbjct: 241 IFCVAISEYDQTLFEDENKNRMMETKELFEWVLKQQCFE---------KTSFMLFLNKFD 300

Query: 309 IFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKV 365
           IFEKK+L VPL+VCEWFKDYQ VSTGKQEIEHAYEFVKKKFEE YFQ+T+PD VDRVFK+
Sbjct: 301 IFEKKILDVPLNVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQSTAPDSVDRVFKI 360

BLAST of Carg10725 vs. ExPASy Swiss-Prot
Match: P93564 (Guanine nucleotide-binding protein alpha-1 subunit OS=Solanum tuberosum OX=4113 GN=GPA1 PE=2 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 3.4e-168
Identity = 301/393 (76.59%), Postives = 325/393 (82.70%), Query Frame = 0

Query: 9   MGLLCSRNRRYNKQDTEENV-------------------------------------QIK 68
           MG LCSRN+ Y++ D EEN                                      QIK
Sbjct: 1   MGSLCSRNKHYSQADDEENTQTAEIERRIEQETKADKHIQKLLLLGAGDSGKSTIFKQIK 60

Query: 69  LLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKL 128
           LLFQTGFDEAELK+YIPVIHANVYQTIK+LHDGSKELAQ++ E SKY+LS+ENK+IGEKL
Sbjct: 61  LLFQTGFDEAELKNYIPVIHANVYQTIKILHDGSKELAQNELEASKYLLSAENKEIGEKL 120

Query: 129 SDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENLQRLSDVNY 188
           S+IGGRLDYPRLT++   DIE LW D AIQET  RGNELQVPDCAHYFMENL+R SD++Y
Sbjct: 121 SEIGGRLDYPRLTKDLVQDIEALWKDPAIQETLLRGNELQVPDCAHYFMENLERFSDIHY 180

Query: 189 IPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV 248
           IPTKEDVL+AR+RTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV
Sbjct: 181 IPTKEDVLFARIRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV 240

Query: 249 IFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFD 308
           IFCAAISEYDQTLFEDE+KNRMMETKELFEWVLKQ CFE         KTSFMLFLNKFD
Sbjct: 241 IFCAAISEYDQTLFEDERKNRMMETKELFEWVLKQPCFE---------KTSFMLFLNKFD 300

Query: 309 IFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKV 365
           IFE+KVL+VPL+ CEWFKDYQ VSTGKQEIEHAYEFVKKKFEE YFQ T+PDRVDRVFK+
Sbjct: 301 IFEQKVLKVPLNTCEWFKDYQSVSTGKQEIEHAYEFVKKKFEESYFQCTAPDRVDRVFKI 360

BLAST of Carg10725 vs. ExPASy Swiss-Prot
Match: P93163 (Guanine nucleotide-binding protein alpha-2 subunit OS=Glycine max OX=3847 GN=GPA2 PE=2 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 3.4e-168
Identity = 301/394 (76.40%), Postives = 325/394 (82.49%), Query Frame = 0

Query: 9   MGLLCSRNRRYNKQDTEENV--------------------------------------QI 68
           MGLLCSRNRRYN  D EEN                                       QI
Sbjct: 1   MGLLCSRNRRYNDADAEENAQTAEIERRIEVRNERAEKHIQKLLLLGAGESGKSTIFKQI 60

Query: 69  KLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEK 128
           KLLFQTGFDEAELKSY+PVIHANVYQTIK+LHDGSKE AQ+D + SKYV+S+ENK+IGEK
Sbjct: 61  KLLFQTGFDEAELKSYLPVIHANVYQTIKLLHDGSKEFAQNDVDSSKYVISNENKEIGEK 120

Query: 129 LSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENLQRLSDVN 188
           L +IGGRLDYP L++E A +IE LW D AIQETYARG+ELQ+PDC  YFMENLQRLSD N
Sbjct: 121 LLEIGGRLDYPYLSKELAQEIENLWKDPAIQETYARGSELQIPDCTDYFMENLQRLSDAN 180

Query: 189 YIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTA 248
           Y+PTKEDVLYARVRTTGVVEIQFSPVGENKKS EVYRLFDVGGQRNERRKWIHLFEGV+A
Sbjct: 181 YVPTKEDVLYARVRTTGVVEIQFSPVGENKKSDEVYRLFDVGGQRNERRKWIHLFEGVSA 240

Query: 249 VIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKF 308
           VIFCAAISEYDQTLFEDE +NRMMETKELFEW+LKQ CFE         KTSFMLFLNKF
Sbjct: 241 VIFCAAISEYDQTLFEDENRNRMMETKELFEWILKQPCFE---------KTSFMLFLNKF 300

Query: 309 DIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFK 365
           DIFEKK+L+VPL+VCEWFKDYQ VSTGKQEIEHAYEFVKKKFEE YFQ+T+PDRVDRVFK
Sbjct: 301 DIFEKKILKVPLNVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQSTAPDRVDRVFK 360

BLAST of Carg10725 vs. ExPASy TrEMBL
Match: A0A6J1FN84 (guanine nucleotide-binding protein alpha-1 subunit OS=Cucurbita moschata OX=3662 GN=LOC111446706 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.6e-192
Identity = 352/401 (87.78%), Postives = 354/401 (88.28%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLSRSMGLLCSRNRRYNKQDTEENV                                
Sbjct: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENVQAAEIERRIVQETEAEKHIQKLLLLGVGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSS+
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSD 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL
Sbjct: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH
Sbjct: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVLQVPLSVCEWFKDYQ +STGKQEIEHAYEFVKKKFEELYFQTTSPD
Sbjct: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQPISTGKQEIEHAYEFVKKKFEELYFQTTSPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 392

BLAST of Carg10725 vs. ExPASy TrEMBL
Match: A0A6J1J2G6 (guanine nucleotide-binding protein alpha-1 subunit OS=Cucurbita maxima OX=3661 GN=LOC111481219 PE=3 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 1.7e-191
Identity = 350/401 (87.28%), Postives = 353/401 (88.03%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLSRSMGLLCSRNRRYNKQDTEENV                                
Sbjct: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENVQDSEIERRIVQETEAEKHIQKLLLLGAGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQ+DKEFSKYVLSSE
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQNDKEFSKYVLSSE 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NKDIGEKLSDIG RLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL
Sbjct: 121 NKDIGEKLSDIGSRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQ+NERRKWIH
Sbjct: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQKNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVLQVPLSVCEWFKDYQ VSTGKQEIEHAYEFVKKKFEELYFQTTSPD
Sbjct: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKL+KKTFKLVDETLRRRNLLEAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLIKKTFKLVDETLRRRNLLEAGLL 392

BLAST of Carg10725 vs. ExPASy TrEMBL
Match: A0A1S3BVH4 (guanine nucleotide-binding protein alpha-1 subunit OS=Cucumis melo OX=3656 GN=LOC103493708 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.1e-182
Identity = 335/401 (83.54%), Postives = 343/401 (85.54%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLSR+MGLLCSRNR YN+QD EEN                                 
Sbjct: 1   MLSHLSRNMGLLCSRNRHYNEQDAEENAQAAEIERRIEQETEAEKHIQKLLLLGAGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQ+DKEF+KYVLSSE
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQNDKEFTKYVLSSE 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NKDIGEKLSDIGGRLDYPRLTRE A DIETLW DAAIQETYARGNELQVPDC  YFMENL
Sbjct: 121 NKDIGEKLSDIGGRLDYPRLTRERAQDIETLWKDAAIQETYARGNELQVPDCTQYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSD NYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH
Sbjct: 181 QRLSDANYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVL+VPLSVCEWF DYQ VSTGKQEIEHAYEFVKKKFEELYF++T+PD
Sbjct: 301 MLFLNKFDIFEKKVLKVPLSVCEWFNDYQPVSTGKQEIEHAYEFVKKKFEELYFKSTAPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNL EAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLFEAGLL 392

BLAST of Carg10725 vs. ExPASy TrEMBL
Match: A0A6J1CDS9 (guanine nucleotide-binding protein alpha-1 subunit OS=Momordica charantia OX=3673 GN=LOC111009792 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.5e-182
Identity = 333/401 (83.04%), Postives = 344/401 (85.79%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLS++MGLLCSRNR YN+QD+EEN                                 
Sbjct: 1   MLSHLSKNMGLLCSRNRHYNEQDSEENAQAAEIERRIEQETEAEKHIQKLLLLGAGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQ+DKEFSKYVL SE
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQNDKEFSKYVLCSE 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NK+IGEKLSDIGGRLDYPRLTRE A DIE LW D AIQETYARGNELQVPDCAHYFMENL
Sbjct: 121 NKEIGEKLSDIGGRLDYPRLTRERAEDIEALWKDGAIQETYARGNELQVPDCAHYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSD NYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH
Sbjct: 181 QRLSDANYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVL+QECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLRQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVL+VPLSVCEWFKDYQ VS+GKQEIEHAYEFVKKKFEELYFQ+T+PD
Sbjct: 301 MLFLNKFDIFEKKVLKVPLSVCEWFKDYQPVSSGKQEIEHAYEFVKKKFEELYFQSTAPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNL EAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLFEAGLL 392

BLAST of Carg10725 vs. ExPASy TrEMBL
Match: A0A0A0L2X2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G648550 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.3e-181
Identity = 333/401 (83.04%), Postives = 342/401 (85.29%), Query Frame = 0

Query: 1   MLSHLSRSMGLLCSRNRRYNKQDTEENV-------------------------------- 60
           MLSHLSR+MGLLCSRNR YN+QD EE                                  
Sbjct: 1   MLSHLSRNMGLLCSRNRHYNEQDAEEKTQAAEIERRIEQETEAEKHIQKLLLLGAGESGK 60

Query: 61  -----QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSE 120
                QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQ+DKEF+KYVLSSE
Sbjct: 61  STIFKQIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQNDKEFTKYVLSSE 120

Query: 121 NKDIGEKLSDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENL 180
           NKDIGEKLSDIGGRLDYPRLTRE A DIETLW DAAIQETY+RGNELQVPDC  YFMENL
Sbjct: 121 NKDIGEKLSDIGGRLDYPRLTRERAQDIETLWKDAAIQETYSRGNELQVPDCTQYFMENL 180

Query: 181 QRLSDVNYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240
           QRLSD NYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH
Sbjct: 181 QRLSDANYIPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIH 240

Query: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSF 300
           LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE         KTSF
Sbjct: 241 LFEGVTAVIFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFE---------KTSF 300

Query: 301 MLFLNKFDIFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPD 360
           MLFLNKFDIFEKKVL+VPLSVCEWF DYQ VSTGKQEIEHAYEFVKKKFEELYF++T+PD
Sbjct: 301 MLFLNKFDIFEKKVLKVPLSVCEWFNDYQPVSTGKQEIEHAYEFVKKKFEELYFKSTAPD 360

Query: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLLEAGLL 365
           RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNL EAGLL
Sbjct: 361 RVDRVFKVYRTTALDQKLVKKTFKLVDETLRRRNLFEAGLL 392

BLAST of Carg10725 vs. TAIR 10
Match: AT2G26300.1 (G protein alpha subunit 1 )

HSP 1 Score: 580.9 bits (1496), Expect = 7.3e-166
Identity = 299/393 (76.08%), Postives = 325/393 (82.70%), Query Frame = 0

Query: 9   MGLLCSRNRRYNKQDTEENV-------------------------------------QIK 68
           MGLLCSR+ R++ +DT+EN                                      QIK
Sbjct: 1   MGLLCSRS-RHHTEDTDENTQAAEIERRIEQEAKAEKHIRKLLLLGAGESGKSTIFKQIK 60

Query: 69  LLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIGEKL 128
           LLFQTGFDE ELKSY+PVIHANVYQTIK+LHDG+KE AQ++ + +KY+LSSE+  IGEKL
Sbjct: 61  LLFQTGFDEGELKSYVPVIHANVYQTIKLLHDGTKEFAQNETDSAKYMLSSESIAIGEKL 120

Query: 129 SDIGGRLDYPRLTREHAHDIETLWNDAAIQETYARGNELQVPDCAHYFMENLQRLSDVNY 188
           S+IGGRLDYPRLT++ A  IETLW D AIQET ARGNELQVPDC  Y MENL+RLSD+NY
Sbjct: 121 SEIGGRLDYPRLTKDIAEGIETLWKDPAIQETCARGNELQVPDCTKYLMENLKRLSDINY 180

Query: 189 IPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV 248
           IPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV
Sbjct: 181 IPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVTAV 240

Query: 249 IFCAAISEYDQTLFEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFD 308
           IFCAAISEYDQTLFEDEQKNRMMETKELF+WVLKQ CFE         KTSFMLFLNKFD
Sbjct: 241 IFCAAISEYDQTLFEDEQKNRMMETKELFDWVLKQPCFE---------KTSFMLFLNKFD 300

Query: 309 IFEKKVLQVPLSVCEWFKDYQLVSTGKQEIEHAYEFVKKKFEELYFQTTSPDRVDRVFKV 365
           IFEKKVL VPL+VCEWF+DYQ VS+GKQEIEHAYEFVKKKFEELY+Q T+PDRVDRVFK+
Sbjct: 301 IFEKKVLDVPLNVCEWFRDYQPVSSGKQEIEHAYEFVKKKFEELYYQNTAPDRVDRVFKI 360

BLAST of Carg10725 vs. TAIR 10
Match: AT1G31930.1 (extra-large GTP-binding protein 3 )

HSP 1 Score: 159.5 bits (402), Expect = 5.3e-39
Identity = 118/382 (30.89%), Postives = 188/382 (49.21%), Query Frame = 0

Query: 29  QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIG 88
           Q K L+   F   EL+    ++ +N+Y+ + +L DG +   +     ++ + + E    G
Sbjct: 449 QAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRERFEEEALSHTRGLNAVEGDSGG 508

Query: 89  EKLSDIG----------------------------GRLD--YPRLTREHAHDIETLWNDA 148
           E+ +D G                            G LD  +P  TRE+A  +E +W D 
Sbjct: 509 EEANDEGTVTTPQSVYTLNPRLKHFSDWLLDIIATGDLDAFFPAATREYAPLVEEVWKDP 568

Query: 149 AIQETYARGNELQ-VPDCAHYFMENLQRLSDVNYIPTKEDVLYAR--VRTTGVVEIQF-- 208
           AIQ TY R +EL  +PD A YF+     +S   Y P++ D++YA    +  G+  ++F  
Sbjct: 569 AIQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSERDIVYAEGVTQGNGLAFMEFSL 628

Query: 209 ---SPVGEN--------KKSGEVYRLFDVGGQ-RNERRKWIHLFEGVTAVIFCAAISEYD 268
              SP+ E+              Y+L  V  +  N+  KW+ +FE V AVIFC ++S+YD
Sbjct: 629 SDHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYD 688

Query: 269 QTLFEDEQ------KNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEK 328
           Q     E       +N+M+++KELFE ++K  CF         + T F+L LNK+D FE+
Sbjct: 689 QINITPESSGTVQYQNKMIQSKELFESMVKHPCF---------KDTPFILILNKYDQFEE 748

Query: 329 KVLQVPLSVCEWFKDYQLVSTGK--QEIEH-AYEFVKKKFEELYFQTTSPDRVDRVFKVY 355
           K+ + PL+ C+WF D+  V T    Q + + AY +V  KF+ LYF  T      ++F V+
Sbjct: 749 KLNRAPLTSCDWFSDFCPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITG----QKLF-VW 808

BLAST of Carg10725 vs. TAIR 10
Match: AT1G31930.2 (extra-large GTP-binding protein 3 )

HSP 1 Score: 159.5 bits (402), Expect = 5.3e-39
Identity = 118/382 (30.89%), Postives = 188/382 (49.21%), Query Frame = 0

Query: 29  QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIG 88
           Q K L+   F   EL+    ++ +N+Y+ + +L DG +   +     ++ + + E    G
Sbjct: 449 QAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRERFEEEALSHTRGLNAVEGDSGG 508

Query: 89  EKLSDIG----------------------------GRLD--YPRLTREHAHDIETLWNDA 148
           E+ +D G                            G LD  +P  TRE+A  +E +W D 
Sbjct: 509 EEANDEGTVTTPQSVYTLNPRLKHFSDWLLDIIATGDLDAFFPAATREYAPLVEEVWKDP 568

Query: 149 AIQETYARGNELQ-VPDCAHYFMENLQRLSDVNYIPTKEDVLYAR--VRTTGVVEIQF-- 208
           AIQ TY R +EL  +PD A YF+     +S   Y P++ D++YA    +  G+  ++F  
Sbjct: 569 AIQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSERDIVYAEGVTQGNGLAFMEFSL 628

Query: 209 ---SPVGEN--------KKSGEVYRLFDVGGQ-RNERRKWIHLFEGVTAVIFCAAISEYD 268
              SP+ E+              Y+L  V  +  N+  KW+ +FE V AVIFC ++S+YD
Sbjct: 629 SDHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYD 688

Query: 269 QTLFEDEQ------KNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEK 328
           Q     E       +N+M+++KELFE ++K  CF         + T F+L LNK+D FE+
Sbjct: 689 QINITPESSGTVQYQNKMIQSKELFESMVKHPCF---------KDTPFILILNKYDQFEE 748

Query: 329 KVLQVPLSVCEWFKDYQLVSTGK--QEIEH-AYEFVKKKFEELYFQTTSPDRVDRVFKVY 355
           K+ + PL+ C+WF D+  V T    Q + + AY +V  KF+ LYF  T      ++F V+
Sbjct: 749 KLNRAPLTSCDWFSDFCPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITG----QKLF-VW 808

BLAST of Carg10725 vs. TAIR 10
Match: AT1G31930.3 (extra-large GTP-binding protein 3 )

HSP 1 Score: 159.5 bits (402), Expect = 5.3e-39
Identity = 118/382 (30.89%), Postives = 188/382 (49.21%), Query Frame = 0

Query: 29  QIKLLFQTGFDEAELKSYIPVIHANVYQTIKVLHDGSKELAQSDKEFSKYVLSSENKDIG 88
           Q K L+   F   EL+    ++ +N+Y+ + +L DG +   +     ++ + + E    G
Sbjct: 449 QAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRERFEEEALSHTRGLNAVEGDSGG 508

Query: 89  EKLSDIG----------------------------GRLD--YPRLTREHAHDIETLWNDA 148
           E+ +D G                            G LD  +P  TRE+A  +E +W D 
Sbjct: 509 EEANDEGTVTTPQSVYTLNPRLKHFSDWLLDIIATGDLDAFFPAATREYAPLVEEVWKDP 568

Query: 149 AIQETYARGNELQ-VPDCAHYFMENLQRLSDVNYIPTKEDVLYAR--VRTTGVVEIQF-- 208
           AIQ TY R +EL  +PD A YF+     +S   Y P++ D++YA    +  G+  ++F  
Sbjct: 569 AIQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSERDIVYAEGVTQGNGLAFMEFSL 628

Query: 209 ---SPVGEN--------KKSGEVYRLFDVGGQ-RNERRKWIHLFEGVTAVIFCAAISEYD 268
              SP+ E+              Y+L  V  +  N+  KW+ +FE V AVIFC ++S+YD
Sbjct: 629 SDHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYD 688

Query: 269 QTLFEDEQ------KNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEK 328
           Q     E       +N+M+++KELFE ++K  CF         + T F+L LNK+D FE+
Sbjct: 689 QINITPESSGTVQYQNKMIQSKELFESMVKHPCF---------KDTPFILILNKYDQFEE 748

Query: 329 KVLQVPLSVCEWFKDYQLVSTGK--QEIEH-AYEFVKKKFEELYFQTTSPDRVDRVFKVY 355
           K+ + PL+ C+WF D+  V T    Q + + AY +V  KF+ LYF  T      ++F V+
Sbjct: 749 KLNRAPLTSCDWFSDFCPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITG----QKLF-VW 808

BLAST of Carg10725 vs. TAIR 10
Match: AT2G23460.1 (extra-large G-protein 1 )

HSP 1 Score: 125.9 bits (315), Expect = 6.4e-29
Identity = 113/378 (29.89%), Postives = 179/378 (47.35%), Query Frame = 0

Query: 29  QIKLLFQ-TGFDEAELKSYIPVIHANVYQTIKVLHDGS---KELAQSDKEFSKYVLSSEN 88
           Q K+L++   F E E ++   +I  NVY  + +L +G    +E A + +   + VL +  
Sbjct: 502 QAKILYKDVPFLEDERENIKVIIQTNVYGYLGMLLEGRERFEEEALALRNTKQCVLENIP 561

Query: 89  KDIGEKLSD--------IGGRLD------------------YPRLTREHAHDIETLWNDA 148
            D G+  S+        IG RL                   +P  +RE+A  +E LW DA
Sbjct: 562 ADEGDAKSNDKTVTMYSIGPRLKAFSDWLLKTMAAGNLGVIFPAASREYAPLVEELWRDA 621

Query: 149 AIQETYARGNEL-QVPDCAHYFMENLQRLSDVNYIPTKEDVLYAR--VRTTGVVEIQFS- 208
           AIQ TY R +EL  +P  A YF+E    +   +Y P+  D+LYA     ++G+  + FS 
Sbjct: 622 AIQATYKRRSELGLLPSVASYFLERAIDVLTPDYEPSDLDILYAEGVTSSSGLACLDFSF 681

Query: 209 ---------PVGENKKSGEVYRLFDVGGQ-RNERRKWIHLFEGVTAVIFCAAISEYDQTL 268
                       ++  S   Y+L  V  +   E  KWI +FE V  V+F  ++S+YDQ  
Sbjct: 682 PQTASEENLDPSDHHDSLLRYQLIRVPSRGLGENCKWIDMFEDVGMVVFVVSMSDYDQV- 741

Query: 269 FEDEQKNRMMETKELFEWVLKQECFEFDAVLFWVQKTSFMLFLNKFDIFEKKVLQVPLSV 328
             ++  N+M+ TK+LFE ++    FE            F+L LNK+D+ E+KV +VPL+ 
Sbjct: 742 -SEDGTNKMLLTKKLFESIITHPIFE---------NMDFLLILNKYDLLEEKVERVPLAR 801

Query: 329 CEWFKDYQLV-------STGKQEI-EHAYEFVKKKFEELYFQTTSPDRVDRVFKVYRTTA 355
           CEWF+D+  V       + G   + + A+ F+  KF+  Y   T      +   V  + +
Sbjct: 802 CEWFQDFNPVVSRHRGSNNGNPTLGQLAFHFMAVKFKRFYSSLTG-----KKLFVSSSKS 861

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037682.16.1e-207100.00Guanine nucleotide-binding protein alpha-1 subunit [Cucurbita argyrosperma subsp... [more]
XP_022941404.13.3e-19287.78guanine nucleotide-binding protein alpha-1 subunit [Cucurbita moschata] >XP_0229... [more]
XP_022982370.13.6e-19187.28guanine nucleotide-binding protein alpha-1 subunit [Cucurbita maxima] >XP_022982... [more]
XP_023524236.14.7e-19187.53guanine nucleotide-binding protein alpha-1 subunit [Cucurbita pepo subsp. pepo][more]
KAG6608333.14.7e-18390.49Guanine nucleotide-binding protein alpha-1 subunit, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
P490821.1e-17178.37Guanine nucleotide-binding protein alpha-1 subunit OS=Lotus japonicus OX=34305 G... [more]
Q402241.3e-17077.86Guanine nucleotide-binding protein alpha-1 subunit OS=Lupinus luteus OX=3873 GN=... [more]
O042782.1e-17077.10Guanine nucleotide-binding protein alpha-1 subunit OS=Pisum sativum OX=3888 GN=G... [more]
P935643.4e-16876.59Guanine nucleotide-binding protein alpha-1 subunit OS=Solanum tuberosum OX=4113 ... [more]
P931633.4e-16876.40Guanine nucleotide-binding protein alpha-2 subunit OS=Glycine max OX=3847 GN=GPA... [more]
Match NameE-valueIdentityDescription
A0A6J1FN841.6e-19287.78guanine nucleotide-binding protein alpha-1 subunit OS=Cucurbita moschata OX=3662... [more]
A0A6J1J2G61.7e-19187.28guanine nucleotide-binding protein alpha-1 subunit OS=Cucurbita maxima OX=3661 G... [more]
A0A1S3BVH41.1e-18283.54guanine nucleotide-binding protein alpha-1 subunit OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1CDS92.5e-18283.04guanine nucleotide-binding protein alpha-1 subunit OS=Momordica charantia OX=367... [more]
A0A0A0L2X21.3e-18183.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G648550 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G26300.17.3e-16676.08G protein alpha subunit 1 [more]
AT1G31930.15.3e-3930.89extra-large GTP-binding protein 3 [more]
AT1G31930.25.3e-3930.89extra-large GTP-binding protein 3 [more]
AT1G31930.35.3e-3930.89extra-large GTP-binding protein 3 [more]
AT2G23460.16.4e-2929.89extra-large G-protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002976Plant G-protein, alpha subunitPRINTSPR01242GPROTEINAPLTcoord: 299..314
score: 98.86
coord: 48..61
score: 90.91
coord: 63..77
score: 67.27
coord: 171..186
score: 97.16
coord: 87..101
score: 89.09
coord: 316..328
score: 79.72
IPR002976Plant G-protein, alpha subunitPANTHERPTHR10218:SF333GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-1 SUBUNITcoord: 29..364
IPR001019Guanine nucleotide binding protein (G-protein), alpha subunitPRINTSPR00318GPROTEINAcoord: 263..272
score: 63.92
coord: 208..236
score: 59.37
coord: 151..173
score: 56.78
coord: 186..203
score: 75.74
IPR001019Guanine nucleotide binding protein (G-protein), alpha subunitSMARTSM00275galpha_1coord: 13..363
e-value: 9.5E-119
score: 410.5
IPR001019Guanine nucleotide binding protein (G-protein), alpha subunitPFAMPF00503G-alphacoord: 29..352
e-value: 1.7E-77
score: 261.0
IPR001019Guanine nucleotide binding protein (G-protein), alpha subunitPANTHERPTHR10218GTP-BINDING PROTEIN ALPHA SUBUNITcoord: 29..364
IPR001019Guanine nucleotide binding protein (G-protein), alpha subunitPROSITEPS51882G_ALPHAcoord: 7..364
score: 70.751205
IPR001019Guanine nucleotide binding protein (G-protein), alpha subunitCDDcd00066G-alphacoord: 29..358
e-value: 1.21459E-124
score: 359.533
IPR011025G protein alpha subunit, helical insertionGENE3D1.10.400.10coord: 39..164
e-value: 1.7E-102
score: 344.8
IPR011025G protein alpha subunit, helical insertionSUPERFAMILY47895Transducin (alpha subunit), insertion domaincoord: 38..163
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 29..352
e-value: 1.7E-102
score: 344.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 158..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10725-RACarg10725-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007186 G protein-coupled receptor signaling pathway
biological_process GO:0007188 adenylate cyclase-modulating G protein-coupled receptor signaling pathway
biological_process GO:0007165 signal transduction
cellular_component GO:0005834 heterotrimeric G-protein complex
molecular_function GO:0031683 G-protein beta/gamma-subunit complex binding
molecular_function GO:0001664 G protein-coupled receptor binding
molecular_function GO:0003924 GTPase activity
molecular_function GO:0019001 guanyl nucleotide binding