Carg10713 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10713
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMechanosensitive ion channel protein
LocationCarg_Chr01: 11827321 .. 11829129 (-)
RNA-Seq ExpressionCarg10713
SyntenyCarg10713
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTTTATGCCCAATTCCTAAAGGTTTCTTTGAACCATTTTTTCACTTTTTGGCGATTATCTCTGTGGGTTGTGATGCGAGTGCTACACAGTTCGGCGAAGGCGGAGCTAAGCTCCTGCGTGAATCTTTAACTTCACTCACTCTGTTCCTCATTTTCCATTTCATTCGGACCCTTCTGGTTTCTCATTCCGCCATTGTTTTTGCTTCCTCTCTTTGTTTTAGTTGATACGAAGTGGGTTTCTGTTTTTATTCATTTTATCTGCTTGTGGCCGCTGTTGCTCTGCATTCAAGAATTGATCTCCGCCTCTGTTTTCCGGTAAGTTGTCCTCTAATAATGTAGTTGTAGGTTTCTTTCTGTGGGTTTTCTTCGATGGAATGTTAGATGAAGTTTTCATTCTTAATCTCTGTGATTCTGATTGCGTCTTGGTTTGTTGTGTTCATACCCCTTTTTCTTTCATTTCGTCTGTTTAGAGCTAAGCTTGGATTCTGATGGATTTGAACGGGAATAAACCTTTGAAACCTATTCGAAGAAGCTCGTCGCAGAAGGAAAGTGAAAATGGTAGCCAAGTAGTTGTTGAAATTAGCAGAGATGAAAATGGGTACTCTGTTCCGAAGCAAAACAGAGTCGATTCACAGACCAAAGAGCCGACGGATTCGAGTATTGGCTATGGCTACGACTCGCATCTCACTCCGACGGCGAATAAACCCCCGAAAATCCCCGGTTCAGTTGGAACCCTCACGCCTAGAAAATCCCTCAAAAGATCAATCCTATCGAAACCCAAATCCAGATTCGGGGAACAATCAAGCTACATCGATTCAGATACGTTTGAAGAGAATCGTTTGTCGTTTAGAGATCAAATCAGTGCAAATTCATCGAGACGTTCTACTCTCAACACGCCGAAGGAACATCATGAGGAAGGAGATGAAGATATTTTCAAGATAGAGAAGTTTAAGAAAGAGAAGCACAAGAAAGTGAAGGTAATAACATTGATTAAATGGGTTGGAGCTTTTTGCATCATTGGTTGCTTGGTAGCTAGCTTGACTATTAAACGTTTGAAGAATTACTTCCTTTGGGGTATAGAGATTTGGAAATGGTGTTTGCTTGCTACTGTGATTTTGTGTGGAATGATATTTACTCATTGGGTTATGAACGTGATTGTGTTCTTGATTGAGAGGAACTTTTTGCTTCAGAAAAAAGTGTTCTATTTTGTTCATGGGTTGAAGAAGAGTGTCCAAGTGACCCTTTGGTTGACATTGGTTCTTGTTACATGGGTGACTTTGTTTGATCGGAGTAGCCATAGGGTCTTGAGATCAAAGATCAGTGGGAAGATTTTGGATGCCATTACATGGACTCTAATAACACTTCTGATAGGAGCATTTTTGTGGTTGATAAAGACATTGTTGTTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATCCATTTTCCATCATCATGTTCTCCAAACCCTTTTGAACCCTTCACCAATTGGCATGCCTGAAAGTACTGTCGAGTCCAACAGTGGTCGATTGAGTTTCAAGGGTAAAAAATCCGACCATAAGAAGGTGATTGACTTGGGAAAGATTCATCAGCTGAAGCGAGAGAAGGTTTCGGCATGGACGATGAAGGTTTTGGTCGATGCAGTTGCAAGTTCGGAGATGTCGATCTCACAATTACTTGACGAAAGCTACCAAGTTGCTGACGGTGAGATCACCGATGAGACGGAGGTTGCTGGATATGCTGCCTTCAAGATCTTCAACAATATTGCTGTTCCTGGAAACGAGTGA

mRNA sequence

AATTTTATGCCCAATTCCTAAAGGTTTCTTTGAACCATTTTTTCACTTTTTGGCGATTATCTCTGTGGGTTGTGATGCGAGTGCTACACAGTTCGGCGAAGGCGGAGCTAAGCTCCTGCGTGAATCTTTAACTTCACTCACTCTGTTCCTCATTTTCCATTTCATTCGGACCCTTCTGGTTTCTCATTCCGCCATTGTTTTTGCTTCCTCTCTTTGTTTTAGTTGATACGAAGTGGGTTTCTGTTTTTATTCATTTTATCTGCTTGTGGCCGCTGTTGCTCTGCATTCAAGAATTGATCTCCGCCTCTGTTTTCCGAGCTAAGCTTGGATTCTGATGGATTTGAACGGGAATAAACCTTTGAAACCTATTCGAAGAAGCTCGTCGCAGAAGGAAAGTGAAAATGGTAGCCAAGTAGTTGTTGAAATTAGCAGAGATGAAAATGGGTACTCTGTTCCGAAGCAAAACAGAGTCGATTCACAGACCAAAGAGCCGACGGATTCGAGTATTGGCTATGGCTACGACTCGCATCTCACTCCGACGGCGAATAAACCCCCGAAAATCCCCGGTTCAGTTGGAACCCTCACGCCTAGAAAATCCCTCAAAAGATCAATCCTATCGAAACCCAAATCCAGATTCGGGGAACAATCAAGCTACATCGATTCAGATACGTTTGAAGAGAATCGTTTGTCGTTTAGAGATCAAATCAGTGCAAATTCATCGAGACGTTCTACTCTCAACACGCCGAAGGAACATCATGAGGAAGGAGATGAAGATATTTTCAAGATAGAGAAGTTTAAGAAAGAGAAGCACAAGAAAGTGAAGGTAATAACATTGATTAAATGGGTTGGAGCTTTTTGCATCATTGGTTGCTTGGTAGCTAGCTTGACTATTAAACGTTTGAAGAATTACTTCCTTTGGGGTATAGAGATTTGGAAATGGTGTTTGCTTGCTACTGTGATTTTGTGTGGAATGATATTTACTCATTGGGTTATGAACGTGATTGTGTTCTTGATTGAGAGGAACTTTTTGCTTCAGAAAAAAGTGTTCTATTTTGTTCATGGGTTGAAGAAGAGTGTCCAAGTGACCCTTTGGTTGACATTGGTTCTTGTTACATGGGTGACTTTGTTTGATCGGAGTAGCCATAGGGTCTTGAGATCAAAGATCAGTGGGAAGATTTTGGATGCCATTACATGGACTCTAATAACACTTCTGATAGGAGCATTTTTGTGGTTGATAAAGACATTGTTGTTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATCCATTTTCCATCATCATGTTCTCCAAACCCTTTTGAACCCTTCACCAATTGGCATGCCTGAAAGTACTGTCGAGTCCAACAGTGGTCGATTGAGTTTCAAGGGTAAAAAATCCGACCATAAGAAGGTGATTGACTTGGGAAAGATTCATCAGCTGAAGCGAGAGAAGGTTTCGGCATGGACGATGAAGGTTTTGGTCGATGCAGTTGCAAGTTCGGAGATGTCGATCTCACAATTACTTGACGAAAGCTACCAAGTTGCTGACGGTGAGATCACCGATGAGACGGAGGTTGCTGGATATGCTGCCTTCAAGATCTTCAACAATATTGCTGTTCCTGGAAACGAGTGA

Coding sequence (CDS)

ATGGATTTGAACGGGAATAAACCTTTGAAACCTATTCGAAGAAGCTCGTCGCAGAAGGAAAGTGAAAATGGTAGCCAAGTAGTTGTTGAAATTAGCAGAGATGAAAATGGGTACTCTGTTCCGAAGCAAAACAGAGTCGATTCACAGACCAAAGAGCCGACGGATTCGAGTATTGGCTATGGCTACGACTCGCATCTCACTCCGACGGCGAATAAACCCCCGAAAATCCCCGGTTCAGTTGGAACCCTCACGCCTAGAAAATCCCTCAAAAGATCAATCCTATCGAAACCCAAATCCAGATTCGGGGAACAATCAAGCTACATCGATTCAGATACGTTTGAAGAGAATCGTTTGTCGTTTAGAGATCAAATCAGTGCAAATTCATCGAGACGTTCTACTCTCAACACGCCGAAGGAACATCATGAGGAAGGAGATGAAGATATTTTCAAGATAGAGAAGTTTAAGAAAGAGAAGCACAAGAAAGTGAAGGTAATAACATTGATTAAATGGGTTGGAGCTTTTTGCATCATTGGTTGCTTGGTAGCTAGCTTGACTATTAAACGTTTGAAGAATTACTTCCTTTGGGGTATAGAGATTTGGAAATGGTGTTTGCTTGCTACTGTGATTTTGTGTGGAATGATATTTACTCATTGGGTTATGAACGTGATTGTGTTCTTGATTGAGAGGAACTTTTTGCTTCAGAAAAAAGTGTTCTATTTTGTTCATGGGTTGAAGAAGAGTGTCCAAGTGACCCTTTGGTTGACATTGGTTCTTGTTACATGGGTGACTTTGTTTGATCGGAGTAGCCATAGGGTCTTGAGATCAAAGATCAGTGGGAAGATTTTGGATGCCATTACATGGACTCTAATAACACTTCTGATAGGAGCATTTTTGTGGTTGATAAAGACATTGTTGTTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATCCATTTTCCATCATCATGTTCTCCAAACCCTTTTGAACCCTTCACCAATTGGCATGCCTGAAAGTACTGTCGAGTCCAACAGTGGTCGATTGAGTTTCAAGGGTAAAAAATCCGACCATAAGAAGGTGATTGACTTGGGAAAGATTCATCAGCTGAAGCGAGAGAAGGTTTCGGCATGGACGATGAAGGTTTTGGTCGATGCAGTTGCAAGTTCGGAGATGTCGATCTCACAATTACTTGACGAAAGCTACCAAGTTGCTGACGGTGAGATCACCGATGAGACGGAGGTTGCTGGATATGCTGCCTTCAAGATCTTCAACAATATTGCTGTTCCTGGAAACGAGTGA

Protein sequence

MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGYGYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSFRDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETEVAGYAAFKIFNNIAVPGNE
Homology
BLAST of Carg10713 vs. NCBI nr
Match: KAG7037694.1 (Mechanosensitive ion channel protein 10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 845.9 bits (2184), Expect = 1.6e-241
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY
Sbjct: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120
           GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120

Query: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180
           RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240
           VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300
           VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360
           IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420
           KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE
Sbjct: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420

Query: 421 VAGYAAFKIFNNIAVPGNE 440
           VAGYAAFKIFNNIAVPGNE
Sbjct: 421 VAGYAAFKIFNNIAVPGNE 439

BLAST of Carg10713 vs. NCBI nr
Match: KAG6608342.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 839.3 bits (2167), Expect = 1.5e-239
Identity = 437/439 (99.54%), Postives = 438/439 (99.77%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY
Sbjct: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120
           GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120

Query: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180
           RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240
           VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTH VMNVIVFLIERNFLLQKKVFYF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300
           VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360
           IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420
           KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE
Sbjct: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420

Query: 421 VAGYAAFKIFNNIAVPGNE 440
           VAGYAAFKIFNNIAVPGN+
Sbjct: 421 VAGYAAFKIFNNIAVPGND 439

BLAST of Carg10713 vs. NCBI nr
Match: XP_022940136.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022940137.1 mechanosensitive ion channel protein 10-like [Cucurbita moschata])

HSP 1 Score: 833.6 bits (2152), Expect = 8.1e-238
Identity = 434/439 (98.86%), Postives = 437/439 (99.54%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEP DSSIGY
Sbjct: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120
           GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120

Query: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180
           RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240
           VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTH VMNVIVFLIERNFLLQKKVFYF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300
           VHGLKKSVQVTLWLTLVLVTWV+LFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360
           IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420
           KS+HKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE
Sbjct: 361 KSNHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420

Query: 421 VAGYAAFKIFNNIAVPGNE 440
           VAGYAAFKIFNNIAVPGN+
Sbjct: 421 VAGYAAFKIFNNIAVPGND 439

BLAST of Carg10713 vs. NCBI nr
Match: XP_023524086.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo] >XP_023524087.1 mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828.2 bits (2138), Expect = 3.4e-236
Identity = 430/439 (97.95%), Postives = 436/439 (99.32%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MD+NGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY
Sbjct: 1   MDVNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120
           GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSS+IDSDTFEENRLS 
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSFIDSDTFEENRLSL 120

Query: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180
           RDQISANSSRRSTLNTPKEHHEEGD+DIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDDDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240
           VASLTIKRLK YFLWGIEIWKWCLLATVILCGMIFTH VMNVIVFLIERNFLLQKKVFYF
Sbjct: 181 VASLTIKRLKKYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300
           VHGLKKSVQVTLWLTLVLVTWV+LFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360
           IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420
           KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE
Sbjct: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420

Query: 421 VAGYAAFKIFNNIAVPGNE 440
           VAGYAAFKIFNNIA+PGN+
Sbjct: 421 VAGYAAFKIFNNIALPGND 439

BLAST of Carg10713 vs. NCBI nr
Match: XP_022982339.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022982340.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 810.1 bits (2091), Expect = 9.6e-231
Identity = 422/439 (96.13%), Postives = 429/439 (97.72%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MDLNGN PLKPIRRSSSQKESENGSQVVVEISRDENG+SVPKQNRVDSQTKEP DSSIGY
Sbjct: 1   MDLNGNTPLKPIRRSSSQKESENGSQVVVEISRDENGHSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120
           GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSS IDSDTFEENRLSF
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSVIDSDTFEENRLSF 120

Query: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180
           RDQISANSSRRSTLN PKEHHEEGD++ FKIEKFKK KHKKVK ITLIKWVGAFCIIGCL
Sbjct: 121 RDQISANSSRRSTLNMPKEHHEEGDDEFFKIEKFKKVKHKKVKAITLIKWVGAFCIIGCL 180

Query: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240
           VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTH VMNVIVFLIERNFLLQKKVFYF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300
           VHGLKKSVQVTLWLTLVLVTWV+LFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360
           IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420
           KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEI DE E
Sbjct: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEIADEME 420

Query: 421 VAGYAAFKIFNNIAVPGNE 440
           VAGYAA+KIF+NIAVPGN+
Sbjct: 421 VAGYAAYKIFDNIAVPGND 439

BLAST of Carg10713 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.6e-79
Identity = 195/460 (42.39%), Postives = 275/460 (59.78%), Query Frame = 0

Query: 17  SQKESENGSQVVVEISRDE-----------NGYSVPKQNRVDSQTKEPTDSSIGYGYDSH 76
           +++   NG +VV+ +S  E           N  + P  +    ++K     SI       
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPEIYK 61

Query: 77  LTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEEN-RLSFRDQI 136
            + + +KPPKIP   G L  RKSL RSI SKPKSRFGEQ S+    T EEN   S R+Q 
Sbjct: 62  FSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQF 121

Query: 137 SA--------------NSSRRS----TLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVIT 196
            A              N S RS     L+   E   + +E+I+K  K  + K   +K + 
Sbjct: 122 GAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKPLA 181

Query: 197 LIKWVGAFCIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFL 256
            ++ V    I+G L+ SLTI  +  + +WG+E WKWC+L  V L GM+ T+W M+ +VF+
Sbjct: 182 FLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFI 241

Query: 257 IERNFLLQKKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAIT 316
           IE+N+LL+KKV YFVHGLKK+VQV +W +LVL+ W+ LFD    R  ++K   + LD IT
Sbjct: 242 IEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTK---RFLDFIT 301

Query: 317 WTLITLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPES 376
           WT+++LL+G+ L+L+KT  LK+LASKF++  FF+RIQES+FH +VLQTL  P  I   E+
Sbjct: 302 WTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAEN 361

Query: 377 TVE-SNSGRLSF---KGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMS-IS 436
                ++G LSF   K  K   KKVID+GK+H++K+EKVSAWTM+VL++AV +S +S IS
Sbjct: 362 VGRVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGISTIS 421

Query: 437 QLLDE---SYQVADGEITDETEVAGYAAFKIFNNIAVPGN 439
             LDE     +  D EIT+E E    AA+ +FNN+A P +
Sbjct: 422 STLDEVNNKKERTDKEITNEMEAVA-AAYDVFNNVAKPNH 456

BLAST of Carg10713 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 8.8e-78
Identity = 206/454 (45.37%), Postives = 273/454 (60.13%), Query Frame = 0

Query: 16  SSQKESE---NGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGYGYDSHLTPTANK 75
           + QK S     G  VV+ +  +E      +    +S+   P   S      S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPSPEI-SKLVGSPNK 61

Query: 76  PPKIP--GSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSFRDQISA---- 135
           PP+ P   +VG LT RKS  RS+ SKPKSRF + S  +D+   EE     R+Q+ A    
Sbjct: 62  PPRAPNQNNVG-LTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAGFSF 121

Query: 136 ------NSSRRSTLN----TPKEHHEEGDED--IFKIEKFKKEKHKKVKVITLIKWVGAF 195
                 N S RS  +    TP +   E DED  I+K  K  +E   K+  + LI+     
Sbjct: 122 SRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFV 181

Query: 196 CIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQ 255
            I+  LVASLTI  LK++  WG+E+WKWC+L  VI  GM+ T+W M +IVFLIE NFLL+
Sbjct: 182 VILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLR 241

Query: 256 KKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLI 315
           +KV YFVHGLKKSVQV +WL L+LV W+ LF   +H V RS  + K+L  IT TLI++L 
Sbjct: 242 RKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILT 301

Query: 316 GAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTV--ESNS 375
           GAF WL+KTLLLKILA+ F++N FFDRIQ+S+FH +VLQT L+  P+      V  E ++
Sbjct: 302 GAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPST 361

Query: 376 GRLSF----KGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMS-ISQLLDES 435
           G LSF    K      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S IS  LDE+
Sbjct: 362 GHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDET 421

Query: 436 -----YQVADGEITDETEVAGYAAFKIFNNIAVP 437
                 + AD EIT E E    AA+ +F N+A P
Sbjct: 422 AYGEGKEQADREITSEMEALA-AAYHVFRNVAQP 445

BLAST of Carg10713 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-44
Identity = 123/342 (35.96%), Postives = 186/342 (54.39%), Query Frame = 0

Query: 136 TPKEHHEEGDEDIFKIEKFKKEKHK-KVKVITLIKWVGAFCIIGCLVASLTIKRLKNYFL 195
           +PK   EE ++D F  E   +E  K K+ +  +++W+    II   V +L I  L+   L
Sbjct: 211 SPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKL 270

Query: 196 WGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYFVHGLKKSVQVTLWL 255
           W +++WKW  +  V++CG + + W++ ++VF IERNFLL+K+V YFV+G++K+VQ  LWL
Sbjct: 271 WELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWL 330

Query: 256 TLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWLIKTLLLKILASKFH 315
            LVL+ W  LFD    +   +    K L  +T   + LL+G  LWL+KTLL+K+LAS FH
Sbjct: 331 GLVLLAWHFLFDEKVAKAANT----KALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 390

Query: 316 MNRFFDRIQESIFHHHVLQTLLNPSPIGMPEST------------------VESNSGR-- 375
           M+ +FDRIQES+F  +V++TL  P  I + ++                   VE  SG   
Sbjct: 391 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQK 450

Query: 376 --------------LSFKGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSI 435
                         LS  G      K I +  +H+L  + VSAW MK L++ + +  ++ 
Sbjct: 451 SPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTT 510

Query: 436 --SQLLDESYQVADG-EITDETEVAGYAAFKIFNNIAVPGNE 440
              QL D S     G +I  E E A  AA KIF+N+A PG++
Sbjct: 511 LDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSK 547

BLAST of Carg10713 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 4.6e-42
Identity = 119/337 (35.31%), Postives = 179/337 (53.12%), Query Frame = 0

Query: 142 EEGDEDIFKIEKFKKE-KHKKVKVITLIKWVGAFCIIGCLVASLTIKRLKNYFLWGIEIW 201
           EE +ED F  E   +E K  K+     ++W+    I+  LV SLTI  L+    W +++W
Sbjct: 244 EEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLW 303

Query: 202 KWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYFVHGLKKSVQVTLWLTLVLVT 261
           KW +   V++CG + + W++ +IVFL+E+NF  +K+V YFV+G++KSVQ  LWL LVL+ 
Sbjct: 304 KWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLA 363

Query: 262 WVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWLIKTLLLKILASKFHMNRFFD 321
           W  LFD+   R  RS      L  +T  L+ LL+   +WL+KT+L+K+LAS FHM+ +FD
Sbjct: 364 WHFLFDKKVERETRS----TALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFD 423

Query: 322 RIQESIFHHHVLQTLLNP------------SPIGMPESTVESNSGRLSFKGKKSDHKKVI 381
           RIQES+F  +V++TL  P              +     ++E  +G       K+  K  +
Sbjct: 424 RIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFM 483

Query: 382 DLGK-------------------IHQLKR---EKVSAWTMKVLVDAVASSEMSISQLLDE 439
            +GK                   I QLKR   + VSAW MK L++ +    +S    LD+
Sbjct: 484 KVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAIS---TLDQ 543

BLAST of Carg10713 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.7e-41
Identity = 139/418 (33.25%), Postives = 207/418 (49.52%), Query Frame = 0

Query: 86  RKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSFRDQISANSSRRSTLN---------- 145
           R +++   L K K+R    S  +D  T      ++ D +S  + R   LN          
Sbjct: 173 RSTMRTKTLMKMKTR----SRLMDPPT-----PTYPDMVSGRTPRSGNLNPGFSGRNTKP 232

Query: 146 -TPKEHHE---EGDEDIFKIEKFKKEKHK-KVKVITLIKWVGAFCIIGCLVASLTIKRLK 205
            TP +      E +ED F  E   +   K K+ V  +I+W+    II  L+ SL I  L+
Sbjct: 233 GTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLR 292

Query: 206 NYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYFVHGLKKSVQV 265
              LW + +WKW ++  V++CG + + W++ + V+ +E NFL +KKV YFV+G++K VQ 
Sbjct: 293 GKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQN 352

Query: 266 TLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWLIKTLLLKILA 325
            LWL LVL+ W  LFD+   R +RS     +L  +T  LI LL+   +WLIKTLL+K+LA
Sbjct: 353 CLWLGLVLIAWHFLFDKKVEREMRS----TVLKYVTKVLICLLVAVIIWLIKTLLVKVLA 412

Query: 326 SKFHMNRFFDRIQESIFHHHVLQTLLNP--------------------------SPIG-- 385
           S FHM+ +FDRIQES+F  +V++TL  P                          SP+G  
Sbjct: 413 SSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPK 472

Query: 386 ----MPESTVESNSGRLSFK--------------GKKSDHKKVIDLGKIHQLKREKVSAW 439
                P+ TV   SGRL                  KK   ++ I +  + ++  + VSAW
Sbjct: 473 AVSSPPQVTV--GSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAW 532

BLAST of Carg10713 vs. ExPASy TrEMBL
Match: A0A6J1FHL2 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111445854 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 3.9e-238
Identity = 434/439 (98.86%), Postives = 437/439 (99.54%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEP DSSIGY
Sbjct: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120
           GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120

Query: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180
           RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL
Sbjct: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180

Query: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240
           VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTH VMNVIVFLIERNFLLQKKVFYF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300
           VHGLKKSVQVTLWLTLVLVTWV+LFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360
           IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420
           KS+HKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE
Sbjct: 361 KSNHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420

Query: 421 VAGYAAFKIFNNIAVPGNE 440
           VAGYAAFKIFNNIAVPGN+
Sbjct: 421 VAGYAAFKIFNNIAVPGND 439

BLAST of Carg10713 vs. ExPASy TrEMBL
Match: A0A6J1J491 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111481199 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.6e-231
Identity = 422/439 (96.13%), Postives = 429/439 (97.72%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MDLNGN PLKPIRRSSSQKESENGSQVVVEISRDENG+SVPKQNRVDSQTKEP DSSIGY
Sbjct: 1   MDLNGNTPLKPIRRSSSQKESENGSQVVVEISRDENGHSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSF 120
           GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSS IDSDTFEENRLSF
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSVIDSDTFEENRLSF 120

Query: 121 RDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGCL 180
           RDQISANSSRRSTLN PKEHHEEGD++ FKIEKFKK KHKKVK ITLIKWVGAFCIIGCL
Sbjct: 121 RDQISANSSRRSTLNMPKEHHEEGDDEFFKIEKFKKVKHKKVKAITLIKWVGAFCIIGCL 180

Query: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYF 240
           VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTH VMNVIVFLIERNFLLQKKVFYF
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 VHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300
           VHGLKKSVQVTLWLTLVLVTWV+LFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360
           IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEITDETE 420
           KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEI DE E
Sbjct: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDESYQVADGEIADEME 420

Query: 421 VAGYAAFKIFNNIAVPGNE 440
           VAGYAA+KIF+NIAVPGN+
Sbjct: 421 VAGYAAYKIFDNIAVPGND 439

BLAST of Carg10713 vs. ExPASy TrEMBL
Match: A0A6J1C8Z8 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009404 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 1.1e-174
Identity = 347/463 (74.95%), Postives = 382/463 (82.51%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEIS--------RDENGYSVPKQNR-VDSQTK 60
           MD+NGNKPLK +RRSSSQKESENG QVVVEIS        RDENG SVP QNR VDSQ K
Sbjct: 1   MDVNGNKPLKAVRRSSSQKESENGGQVVVEISGVVPSKETRDENGCSVPSQNRVVDSQNK 60

Query: 61  EPTDSSIGYGYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSS-YIDS 120
            PTDSSIG         +ANKPPKIP S GTL PRKSLKRSI SKPKSRFGEQ   YIDS
Sbjct: 61  GPTDSSIG---------SANKPPKIPNSSGTLMPRKSLKRSIQSKPKSRFGEQQPYYIDS 120

Query: 121 DTFEENRLSFRDQISANSSRR------STLNTPKEHHEEGDED-IFKIEKFKKEKHKKVK 180
           D FEE+RLS R+QI A SSRR      S L TP+   EE DED I+K E+  KEKHKK+K
Sbjct: 121 DMFEEDRLSLREQIGATSSRRASPGHKSALTTPRAQAEEEDEDAIWKTEQLSKEKHKKLK 180

Query: 181 VITLIKWVGAFCIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVI 240
           + TL+KWVG FCIIGCLVASLT+ RL+N FLWG+EIWKWCLLATVILCGMIFT WVMNVI
Sbjct: 181 MKTLVKWVGVFCIIGCLVASLTVNRLRNCFLWGLEIWKWCLLATVILCGMIFTKWVMNVI 240

Query: 241 VFLIERNFLLQKKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILD 300
           VFLIERNFLL+KKV YFVHGLKK VQVTLWLTLVL TW +LFDRS+HRVLRSK +GKILD
Sbjct: 241 VFLIERNFLLKKKVLYFVHGLKKIVQVTLWLTLVLATWASLFDRSNHRVLRSKTAGKILD 300

Query: 301 AITWTLITLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGM 360
           A TWTL++LLIGAFLWL+KTLLLKILASKFHMNRFFDRIQESIFHHHVLQTL  P  +G+
Sbjct: 301 AGTWTLVSLLIGAFLWLVKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLSRPPLMGV 360

Query: 361 PE--STVESNSGRLSFKGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSIS 420
            E  ++ +S+SGRLS KGKKSDHKKVID+GKIHQLKREKVSAWTMKVLVDAV SSE+SIS
Sbjct: 361 AECAASAKSSSGRLSLKGKKSDHKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSELSIS 420

Query: 421 QLLDESYQ-----VADGEITDETEVAGYAAFKIFNNIAVPGNE 440
           QLLDESYQ     VAD +ITDE EVA  AA++IFNN+A+PGN+
Sbjct: 421 QLLDESYQNGGAEVADSDITDEMEVARAAAYRIFNNVALPGNK 454

BLAST of Carg10713 vs. ExPASy TrEMBL
Match: A0A1S4DZ48 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.7e-162
Identity = 318/442 (71.95%), Postives = 360/442 (81.45%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MD+NGN   KPIRRSSSQKESENG +VVV++S  EN YSVPKQNRVDSQTKEPT SS+GY
Sbjct: 1   MDVNGN---KPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTF-EENRLS 120
           G  S L PTANKPPKIP S GTLTPR+SL+RS LSKPKSRFGEQ  YIDSD F EEN +S
Sbjct: 61  GNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVS 120

Query: 121 FRDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGC 180
            R+QI A SSR S LNTPK   E  D     +     EKHKK KV T+ KW+G FCII C
Sbjct: 121 LREQIGATSSRSSNLNTPKAQPEGEDGRNMGL----NEKHKKAKVKTVFKWIGVFCIISC 180

Query: 181 LVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFY 240
           LVASLT+K LKN FLWG+++WKWCLLATVI CG+IFT W+MNV+VFLIERNFLL+KKV Y
Sbjct: 181 LVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLY 240

Query: 241 FVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLW 300
           FVHGLKKSVQVTLWL+LVL TW +LFDR +H +  S+I+ KILDA+TWTL +LLIGAFLW
Sbjct: 241 FVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLW 300

Query: 301 LIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKG 360
           LIKTLLLKILASKFHMNRFFDRIQES+F HHVLQTLL P P+   EST +    R  F+ 
Sbjct: 301 LIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPV-EDESTAKFRCCRFCFES 360

Query: 361 KKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLL-DESYQ-VADGEITD 420
           K+SD KKVID+GKIHQLKREKVSAWTMKVLVDAV SSEMSISQ+L DESY+ VADG+IT+
Sbjct: 361 KESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITN 420

Query: 421 ETEVAGYAAFKIFNNIAVPGNE 440
           E  VA  AA +IF N+A+PGN+
Sbjct: 421 EMNVAKEAAKEIFKNVALPGNK 434

BLAST of Carg10713 vs. ExPASy TrEMBL
Match: A0A5D3D991 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001820 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.7e-162
Identity = 318/442 (71.95%), Postives = 360/442 (81.45%), Query Frame = 0

Query: 1   MDLNGNKPLKPIRRSSSQKESENGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGY 60
           MD+NGN   KPIRRSSSQKESENG +VVV++S  EN YSVPKQNRVDSQTKEPT SS+GY
Sbjct: 1   MDVNGN---KPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGY 60

Query: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTF-EENRLS 120
           G  S L PTANKPPKIP S GTLTPR+SL+RS LSKPKSRFGEQ  YIDSD F EEN +S
Sbjct: 61  GNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVS 120

Query: 121 FRDQISANSSRRSTLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVITLIKWVGAFCIIGC 180
            R+QI A SSR S LNTPK   E  D     +     EKHKK KV T+ KW+G FCII C
Sbjct: 121 LREQIGATSSRSSNLNTPKAQPEGEDGRNMGL----NEKHKKAKVKTVFKWIGVFCIISC 180

Query: 181 LVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFY 240
           LVASLT+K LKN FLWG+++WKWCLLATVI CG+IFT W+MNV+VFLIERNFLL+KKV Y
Sbjct: 181 LVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLY 240

Query: 241 FVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLW 300
           FVHGLKKSVQVTLWL+LVL TW +LFDR +H +  S+I+ KILDA+TWTL +LLIGAFLW
Sbjct: 241 FVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLW 300

Query: 301 LIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKG 360
           LIKTLLLKILASKFHMNRFFDRIQES+F HHVLQTLL P P+   EST +    R  F+ 
Sbjct: 301 LIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPV-EDESTAKFRCCRFCFES 360

Query: 361 KKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLL-DESYQ-VADGEITD 420
           K+SD KKVID+GKIHQLKREKVSAWTMKVLVDAV SSEMSISQ+L DESY+ VADG+IT+
Sbjct: 361 KESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITN 420

Query: 421 ETEVAGYAAFKIFNNIAVPGNE 440
           E  VA  AA +IF N+A+PGN+
Sbjct: 421 EMNVAKEAAKEIFKNVALPGNK 434

BLAST of Carg10713 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-80
Identity = 195/460 (42.39%), Postives = 275/460 (59.78%), Query Frame = 0

Query: 17  SQKESENGSQVVVEISRDE-----------NGYSVPKQNRVDSQTKEPTDSSIGYGYDSH 76
           +++   NG +VV+ +S  E           N  + P  +    ++K     SI       
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPEIYK 61

Query: 77  LTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEEN-RLSFRDQI 136
            + + +KPPKIP   G L  RKSL RSI SKPKSRFGEQ S+    T EEN   S R+Q 
Sbjct: 62  FSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQF 121

Query: 137 SA--------------NSSRRS----TLNTPKEHHEEGDEDIFKIEKFKKEKHKKVKVIT 196
            A              N S RS     L+   E   + +E+I+K  K  + K   +K + 
Sbjct: 122 GAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKPLA 181

Query: 197 LIKWVGAFCIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFL 256
            ++ V    I+G L+ SLTI  +  + +WG+E WKWC+L  V L GM+ T+W M+ +VF+
Sbjct: 182 FLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFI 241

Query: 257 IERNFLLQKKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAIT 316
           IE+N+LL+KKV YFVHGLKK+VQV +W +LVL+ W+ LFD    R  ++K   + LD IT
Sbjct: 242 IEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTK---RFLDFIT 301

Query: 317 WTLITLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPES 376
           WT+++LL+G+ L+L+KT  LK+LASKF++  FF+RIQES+FH +VLQTL  P  I   E+
Sbjct: 302 WTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAEN 361

Query: 377 TVE-SNSGRLSF---KGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMS-IS 436
                ++G LSF   K  K   KKVID+GK+H++K+EKVSAWTM+VL++AV +S +S IS
Sbjct: 362 VGRVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGISTIS 421

Query: 437 QLLDE---SYQVADGEITDETEVAGYAAFKIFNNIAVPGN 439
             LDE     +  D EIT+E E    AA+ +FNN+A P +
Sbjct: 422 STLDEVNNKKERTDKEITNEMEAVA-AAYDVFNNVAKPNH 456

BLAST of Carg10713 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 292.4 bits (747), Expect = 6.3e-79
Identity = 206/454 (45.37%), Postives = 273/454 (60.13%), Query Frame = 0

Query: 16  SSQKESE---NGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGYGYDSHLTPTANK 75
           + QK S     G  VV+ +  +E      +    +S+   P   S      S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPSPEI-SKLVGSPNK 61

Query: 76  PPKIP--GSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSFRDQISA---- 135
           PP+ P   +VG LT RKS  RS+ SKPKSRF + S  +D+   EE     R+Q+ A    
Sbjct: 62  PPRAPNQNNVG-LTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAGFSF 121

Query: 136 ------NSSRRSTLN----TPKEHHEEGDED--IFKIEKFKKEKHKKVKVITLIKWVGAF 195
                 N S RS  +    TP +   E DED  I+K  K  +E   K+  + LI+     
Sbjct: 122 SRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFV 181

Query: 196 CIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQ 255
            I+  LVASLTI  LK++  WG+E+WKWC+L  VI  GM+ T+W M +IVFLIE NFLL+
Sbjct: 182 VILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLR 241

Query: 256 KKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLI 315
           +KV YFVHGLKKSVQV +WL L+LV W+ LF   +H V RS  + K+L  IT TLI++L 
Sbjct: 242 RKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILT 301

Query: 316 GAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTV--ESNS 375
           GAF WL+KTLLLKILA+ F++N FFDRIQ+S+FH +VLQT L+  P+      V  E ++
Sbjct: 302 GAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPST 361

Query: 376 GRLSF----KGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMS-ISQLLDES 435
           G LSF    K      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S IS  LDE+
Sbjct: 362 GHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDET 421

Query: 436 -----YQVADGEITDETEVAGYAAFKIFNNIAVP 437
                 + AD EIT E E    AA+ +F N+A P
Sbjct: 422 AYGEGKEQADREITSEMEALA-AAYHVFRNVAQP 445

BLAST of Carg10713 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 292.4 bits (747), Expect = 6.3e-79
Identity = 206/454 (45.37%), Postives = 273/454 (60.13%), Query Frame = 0

Query: 16  SSQKESE---NGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGYGYDSHLTPTANK 75
           + QK S     G  VV+ +  +E      +    +S+   P   S      S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPSPEI-SKLVGSPNK 61

Query: 76  PPKIP--GSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSFRDQISA---- 135
           PP+ P   +VG LT RKS  RS+ SKPKSRF + S  +D+   EE     R+Q+ A    
Sbjct: 62  PPRAPNQNNVG-LTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAGFSF 121

Query: 136 ------NSSRRSTLN----TPKEHHEEGDED--IFKIEKFKKEKHKKVKVITLIKWVGAF 195
                 N S RS  +    TP +   E DED  I+K  K  +E   K+  + LI+     
Sbjct: 122 SRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFV 181

Query: 196 CIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQ 255
            I+  LVASLTI  LK++  WG+E+WKWC+L  VI  GM+ T+W M +IVFLIE NFLL+
Sbjct: 182 VILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLR 241

Query: 256 KKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLI 315
           +KV YFVHGLKKSVQV +WL L+LV W+ LF   +H V RS  + K+L  IT TLI++L 
Sbjct: 242 RKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILT 301

Query: 316 GAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTV--ESNS 375
           GAF WL+KTLLLKILA+ F++N FFDRIQ+S+FH +VLQT L+  P+      V  E ++
Sbjct: 302 GAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPST 361

Query: 376 GRLSF----KGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMS-ISQLLDES 435
           G LSF    K      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S IS  LDE+
Sbjct: 362 GHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDET 421

Query: 436 -----YQVADGEITDETEVAGYAAFKIFNNIAVP 437
                 + AD EIT E E    AA+ +F N+A P
Sbjct: 422 AYGEGKEQADREITSEMEALA-AAYHVFRNVAQP 445

BLAST of Carg10713 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 292.4 bits (747), Expect = 6.3e-79
Identity = 206/454 (45.37%), Postives = 273/454 (60.13%), Query Frame = 0

Query: 16  SSQKESE---NGSQVVVEISRDENGYSVPKQNRVDSQTKEPTDSSIGYGYDSHLTPTANK 75
           + QK S     G  VV+ +  +E      +    +S+   P   S      S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPSPEI-SKLVGSPNK 61

Query: 76  PPKIP--GSVGTLTPRKSLKRSILSKPKSRFGEQSSYIDSDTFEENRLSFRDQISA---- 135
           PP+ P   +VG LT RKS  RS+ SKPKSRF + S  +D+   EE     R+Q+ A    
Sbjct: 62  PPRAPNQNNVG-LTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAGFSF 121

Query: 136 ------NSSRRSTLN----TPKEHHEEGDED--IFKIEKFKKEKHKKVKVITLIKWVGAF 195
                 N S RS  +    TP +   E DED  I+K  K  +E   K+  + LI+     
Sbjct: 122 SRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFV 181

Query: 196 CIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQ 255
            I+  LVASLTI  LK++  WG+E+WKWC+L  VI  GM+ T+W M +IVFLIE NFLL+
Sbjct: 182 VILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLR 241

Query: 256 KKVFYFVHGLKKSVQVTLWLTLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLI 315
           +KV YFVHGLKKSVQV +WL L+LV W+ LF   +H V RS  + K+L  IT TLI++L 
Sbjct: 242 RKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILT 301

Query: 316 GAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTV--ESNS 375
           GAF WL+KTLLLKILA+ F++N FFDRIQ+S+FH +VLQT L+  P+      V  E ++
Sbjct: 302 GAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPST 361

Query: 376 GRLSF----KGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMS-ISQLLDES 435
           G LSF    K      KKVID+GK+H++KREKVSAWTM+VL++AV +S +S IS  LDE+
Sbjct: 362 GHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDET 421

Query: 436 -----YQVADGEITDETEVAGYAAFKIFNNIAVP 437
                 + AD EIT E E    AA+ +F N+A P
Sbjct: 422 AYGEGKEQADREITSEMEALA-AAYHVFRNVAQP 445

BLAST of Carg10713 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-45
Identity = 123/342 (35.96%), Postives = 186/342 (54.39%), Query Frame = 0

Query: 136 TPKEHHEEGDEDIFKIEKFKKEKHK-KVKVITLIKWVGAFCIIGCLVASLTIKRLKNYFL 195
           +PK   EE ++D F  E   +E  K K+ +  +++W+    II   V +L I  L+   L
Sbjct: 211 SPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKL 270

Query: 196 WGIEIWKWCLLATVILCGMIFTHWVMNVIVFLIERNFLLQKKVFYFVHGLKKSVQVTLWL 255
           W +++WKW  +  V++CG + + W++ ++VF IERNFLL+K+V YFV+G++K+VQ  LWL
Sbjct: 271 WELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWL 330

Query: 256 TLVLVTWVTLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWLIKTLLLKILASKFH 315
            LVL+ W  LFD    +   +    K L  +T   + LL+G  LWL+KTLL+K+LAS FH
Sbjct: 331 GLVLLAWHFLFDEKVAKAANT----KALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFH 390

Query: 316 MNRFFDRIQESIFHHHVLQTLLNPSPIGMPEST------------------VESNSGR-- 375
           M+ +FDRIQES+F  +V++TL  P  I + ++                   VE  SG   
Sbjct: 391 MSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQK 450

Query: 376 --------------LSFKGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSI 435
                         LS  G      K I +  +H+L  + VSAW MK L++ + +  ++ 
Sbjct: 451 SPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTT 510

Query: 436 --SQLLDESYQVADG-EITDETEVAGYAAFKIFNNIAVPGNE 440
              QL D S     G +I  E E A  AA KIF+N+A PG++
Sbjct: 511 LDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSK 547

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037694.11.6e-241100.00Mechanosensitive ion channel protein 10 [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6608342.11.5e-23999.54Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022940136.18.1e-23898.86mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022940137.... [more]
XP_023524086.13.4e-23697.95mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo] >XP_02... [more]
XP_022982339.19.6e-23196.13mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022982340.1 ... [more]
Match NameE-valueIdentityDescription
Q84M971.6e-7942.39Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Q9LYG98.8e-7845.37Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
Q9SYM11.7e-4435.96Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LH744.6e-4235.31Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
Q9LPG31.7e-4133.25Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
Match NameE-valueIdentityDescription
A0A6J1FHL23.9e-23898.86Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC1114458... [more]
A0A6J1J4914.6e-23196.13Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111481199... [more]
A0A6J1C8Z81.1e-17474.95Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A1S4DZ482.7e-16271.95Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=... [more]
A0A5D3D9912.7e-16271.95Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT5G19520.11.1e-8042.39mechanosensitive channel of small conductance-like 9 [more]
AT5G12080.16.3e-7945.37mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.26.3e-7945.37mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.36.3e-7945.37mechanosensitive channel of small conductance-like 10 [more]
AT1G78610.11.2e-4535.96mechanosensitive channel of small conductance-like 6 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..84
NoneNo IPR availablePANTHERPTHR31618:SF17MECHANOSENSITIVE ION CHANNEL PROTEIN 9coord: 18..437
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 18..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10713-RACarg10713-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0050982 detection of mechanical stimulus
biological_process GO:0034220 ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0008381 mechanosensitive ion channel activity