Carg10621 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10621
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein DCL homolog, chloroplastic-like
Location	Carg_Chr01: 12259299 .. 12261127 (-)
RNA-Seq Expression	Carg10621
Synteny	Carg10621

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCTTCTTTGCTGCTCAGGGGGCATCCTCTCATTCGGTTAGGGCTCAGGAACCGCGGGCTATGTACTGGGATCGTGCAGGTGACTCGCCGGTCTTGCTGCACTGCGATGCCGGCGTCTACTCCACCGGGCGGCGGCTTATCATCTGCTGAAAATACTACCTCTGTACTGAGTGCCACTGACCCACCGAAGTACCAAAGGTGGGATGAGCCTGGTTATAGAAAGTGGAAGAATCAGGAAGAGGAAATTCTCAGCGATATCGACCCTGTCATATCCCTCACAAAGGAGATCCTCCACTCCAATAGGTTTGTCCTGTTCGCGTAATTTCACCCCGAAAGTTTATACTTTATGTTATGGGCCTTATGTGTTTGCTTTAACTTTTTAATAAATGATCACAGTTGTTAGAATTTCCTTATTCCCAGTTTTACTCAATGTGATATGTTATATAGTTTTTTTTTCCTTTTTTGTTAGTATTACAAATGTGAATTATGAGTATGTAAACTTAGGGAGTACATGTCAAGTACTATTGAGTTTCATAATTTGTGTATTCATAAAAATATGATAACCACTTTCAAATTATTCAATGAGGTCGGCACAATCTAGCGGCCTCTTAATAACCGTCGGATGTAGACCTAAAACTAGACATGGATACAAAGGTAAAAGGGTTTTACAATCTCTTTCACCCTTCTAATGGCGTTAAACTGGCAATCATATTGTAGAGGCTTCCTCAGCAACTACGGGTAAGCTTTCAAAGACATTATAGTAGTAAAAAAAAAAAATCAATAATGGAATTGGGTTTAGTGTAAGTATGTTGTATGTGCCCTTTCTTAATTTTCTTCATGGATTTGGGATTTGATTTTCTGCTTCTTCTGCAAAGAACTGCCTCTGAGTGTAATATATACCTTAAGGGGAGCATACCTCTTGCAAGGTTGGCATGTTCATGAGCTGGTCTCATTAGGCACTTCTCCATATGATATATTGCTCATGTTTTCGTTTTACTATCACATTTTTCTCCTAAGATATTATGTAGCCTTCTCTTAAGAACCTCTATTTTGTATACTAGGTATGTCGATGGGGAGCGATTGACATTTGAGGATGAGAAAATTGTGGTTGACAGGCTTCTTGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGCCTTGAATCCATTATGGTACGCTTTGATCTGGACTAAATGGTTTATACTTCTTACTATGTTTCAAATATGCAGTCGCTTGCTTTTGTTTGTTAGGCCGTATAGAAGTAAAAAGCAGGTAAAGGAACAAATAACGTTTGGTATATGATATTCTCAAAATCTCAAAATACCACCTCTTACCTATAACATTTGTAGCACGCCTCCTTAAGTTTCTGCCCGTGTGGTCATGTAATGGCCTCCAATTGTCACCCAACTCCTTCCCAATAAGGGGTAGATTTGAAACGTGCTTGAAGTTTGCAAGGACTGGGAATCAACCTGTATTTTGTGTGCTTTTTTTTAAGGAATGTGGAGAGAGATTGATGGCCCTCTCGAACTAATCATTGGGAGACGAGTTGTTTGTCATATTTCTTTGTGAATTTTGTGAATAGAAAAGGGATTCCTAGCACACTTTTGATATCAACAAAGCTAACTTGTATAAATTATTTATGCTATTTTATGTGGTTGAAGGTCGATCGGCACCCCCAATTTCGGCACTCCAGATGCCTCTTTGTCATAAGGACCGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAATACCCATTATATGCAGAGCGGTTTATTCGGGAACATTTCAAACGT

mRNA sequence

ATGGTTGCTTCTTTGCTGCTCAGGGGGCATCCTCTCATTCGGTTAGGGCTCAGGAACCGCGGGCTATGTACTGGGATCGTGCAGGTGACTCGCCGGTCTTGCTGCACTGCGATGCCGGCGTCTACTCCACCGGGCGGCGGCTTATCATCTGCTGAAAATACTACCTCTGTACTGAGTGCCACTGACCCACCGAAGTACCAAAGGTGGGATGAGCCTGGTTATAGAAAGTGGAAGAATCAGGAAGAGGAAATTCTCAGCGATATCGACCCTGTCATATCCCTCACAAAGGAGATCCTCCACTCCAATAGGTATGTCGATGGGGAGCGATTGACATTTGAGGATGAGAAAATTGTGGTTGACAGGCTTCTTGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGCCTTGAATCCATTATGGTCGATCGGCACCCCCAATTTCGGCACTCCAGATGCCTCTTTGTCATAAGGACCGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAATACCCATTATATGCAGAGCGGTTTATTCGGGAACATTTCAAACGT

Coding sequence (CDS)

ATGGTTGCTTCTTTGCTGCTCAGGGGGCATCCTCTCATTCGGTTAGGGCTCAGGAACCGCGGGCTATGTACTGGGATCGTGCAGGTGACTCGCCGGTCTTGCTGCACTGCGATGCCGGCGTCTACTCCACCGGGCGGCGGCTTATCATCTGCTGAAAATACTACCTCTGTACTGAGTGCCACTGACCCACCGAAGTACCAAAGGTGGGATGAGCCTGGTTATAGAAAGTGGAAGAATCAGGAAGAGGAAATTCTCAGCGATATCGACCCTGTCATATCCCTCACAAAGGAGATCCTCCACTCCAATAGGTATGTCGATGGGGAGCGATTGACATTTGAGGATGAGAAAATTGTGGTTGACAGGCTTCTTGCTCATCATCCACATGCTGAAGATAAAATTGGATGTGGCCTTGAATCCATTATGGTCGATCGGCACCCCCAATTTCGGCACTCCAGATGCCTCTTTGTCATAAGGACCGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAATACCCATTATATGCAGAGCGGTTTATTCGGGAACATTTCAAACGT

Protein sequence

MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSATDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYAERFIREHFKR

Homology

[Close]

BLAST of Carg10621 vs. NCBI nr
Match: KAG7037785.1 (Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 405.2 bits (1040), Expect = 3.1e-109
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           PLYAERFIREHFKR
Sbjct: 181 PLYAERFIREHFKR 194

[Close]

BLAST of Carg10621 vs. NCBI nr
Match: KAG6608449.1 (Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 405.2 bits (1040), Expect = 3.1e-109
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           PLYAERFIREHFKR
Sbjct: 181 PLYAERFIREHFKR 194

[Close]

BLAST of Carg10621 vs. NCBI nr
Match: XP_022982288.1 (protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 401.0 bits (1029), Expect = 5.9e-108
Identity = 190/194 (97.94%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           MVASLLLRGHPLIRLGLRNRGLCTGI+QVTRRSCCTAMPASTPPGGGLSSAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDP+ISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           PLYA+RFIREHFKR
Sbjct: 181 PLYADRFIREHFKR 194

[Close]

BLAST of Carg10621 vs. NCBI nr
Match: XP_023523235.1 (protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.8 bits (1026), Expect = 1.3e-107
Identity = 189/194 (97.42%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           MVASLLLRGHPLIRLGLRNRGLCTG+V+VTRRSCCTAMPASTPPGGGLSSAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGVVRVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPP YQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKI+VD
Sbjct: 61  TDPPNYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIMVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFV+RTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           PLYAERFIREHFKR
Sbjct: 181 PLYAERFIREHFKR 194

[Close]

BLAST of Carg10621 vs. NCBI nr
Match: XP_022940238.1 (protein DCL homolog, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 399.4 bits (1025), Expect = 1.7e-107
Identity = 190/194 (97.94%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           MVASLLLRGHPLIRLGLRNRGLC GIVQVTRRSCCTA+PASTPPGGGLSSAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRW+EPGYRKWKNQEEEILSDIDP+ISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           PLYAERFIREHFKR
Sbjct: 181 PLYAERFIREHFKR 194

[Close]

BLAST of Carg10621 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.7e-29
Identity = 53/114 (46.49%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 81  EEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESI 140
           E++IL    P++   + ILHS +Y + +RL+ E E+ +++ LL +HP  E KIGCG++ I
Sbjct: 101 EDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYI 160

Query: 141 MVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYAERFIREHFKR 195
           MV  HP F  SRC+F++R DG  +DFSY KC++  I+ KYPLYA+ FI  HF++
Sbjct: 161 MVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of Carg10621 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-28
Identity = 53/118 (44.92%), Postives = 80/118 (67.80%), Query Frame = 0


Query: 77  WKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCG 136
           W + E++IL D  P++   + ILHS +Y  G+RL+ + ++ ++ RLL +HP  + KIG G
Sbjct: 102 WVDWEDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKKIGPG 161

Query: 137 LESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYAERFIREHFKR 195
           ++ I V  HP F +SRCLF++R DG  +DFSY KC++  IR  YPLYA+ FI  HF++
Sbjct: 162 VDYITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHFRK 219

[Close]

BLAST of Carg10621 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.2e-24
Identity = 56/145 (38.62%), Postives = 90/145 (62.07%), Query Frame = 0


Query: 50   SAENTTSVLSATDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGER 109
            S +N   + +AT     QR D      + ++E+E+LSD++PV+   ++I+H + Y DG+ 
Sbjct: 1714 SEDNLAPMFTAT----RQRLD-----SFTSEEQELLSDVEPVMRTLRKIMHPSAYPDGDP 1773

Query: 110  LTFEDEKIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQ 169
            ++ +D+  V++++L  HP  E K+G G++ I VD+H  F  SRC FV+ TDG   DFSY+
Sbjct: 1774 ISDDDKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYR 1833

Query: 170  KCLRAYIRNKYPLYAERFIREHFKR 195
            K L  Y+  KYP  AE FI ++F +
Sbjct: 1834 KSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of Carg10621 vs. ExPASy Swiss-Prot
Match: Q9LQ02 (DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 8.9e-06
Identity = 36/96 (37.50%), Postives = 50/96 (52.08%), Query Frame = 0


Query: 77   WKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCG 136
            WKN        I+ +    K ILHS  Y   E L   DE + V  +L  HP++ +KIG G
Sbjct: 1336 WKN--------IELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPG 1395

Query: 137  LESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCL 173
            ++ I V +  +   S C  V+R DG + DFSY KC+
Sbjct: 1396 VKGIRVAK-SKHGDSCCFEVVRIDGTFEDFSYHKCV 1419

[Close]

BLAST of Carg10621 vs. ExPASy TrEMBL
Match: A0A6J1J4G4 (protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481165 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.9e-108
Identity = 190/194 (97.94%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           MVASLLLRGHPLIRLGLRNRGLCTGI+QVTRRSCCTAMPASTPPGGGLSSAENTTSVLS 
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRWDEPGYRKWKNQEEEILSDIDP+ISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           PLYA+RFIREHFKR
Sbjct: 181 PLYADRFIREHFKR 194

[Close]

BLAST of Carg10621 vs. ExPASy TrEMBL
Match: A0A6J1FNQ4 (protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445919 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 8.3e-108
Identity = 190/194 (97.94%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           MVASLLLRGHPLIRLGLRNRGLC GIVQVTRRSCCTA+PASTPPGGGLSSAENTTSVLSA
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           TDPPKYQRW+EPGYRKWKNQEEEILSDIDP+ISLTKEILHSNRYVDGERLTFEDEKIVVD
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           PLYAERFIREHFKR
Sbjct: 181 PLYAERFIREHFKR 194

[Close]

BLAST of Carg10621 vs. ExPASy TrEMBL
Match: A0A6J1C4J4 (protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007322 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.3e-96
Identity = 170/194 (87.63%), Postives = 182/194 (93.81%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           M ASLLLRGHPL+RLGLR+RGLCTGIVQVTRRSCCTA  A TPP G L+SAEN TSVLS+
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           +DPPKY RWDEP YRKWK+QEEE+L+DI+P+ISLTKEILHSNRYVDGERLT  DE+IVV+
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           P YAERFIREHFKR
Sbjct: 181 PSYAERFIREHFKR 194

[Close]

BLAST of Carg10621 vs. ExPASy TrEMBL
Match: A0A1S3BU88 (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.2e-91
Identity = 158/194 (81.44%), Postives = 175/194 (90.21%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           M AS L RGHPL+RLGL+ RGLCTG+VQVTRRSCC+A  ASTP  G L++ +N TS++S+
Sbjct: 1   MAASFLFRGHPLLRLGLKRRGLCTGVVQVTRRSCCSATVASTPSDGDLTAGDNATSLVSS 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           ++PPKYQRWDEP YRKWKNQEEEIL DI+P+I LTKEILHS+RY DGERLT EDE+ VVD
Sbjct: 61  SEPPKYQRWDEPDYRKWKNQEEEILGDIEPIIFLTKEILHSHRYADGERLTLEDERTVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRC FVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCFFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PLYAERFIREHFKR 195
           P +AERFIREHFKR
Sbjct: 181 PSFAERFIREHFKR 194

[Close]

BLAST of Carg10621 vs. ExPASy TrEMBL
Match: A0A6J1IHJ1 (protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477278 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.6e-90
Identity = 161/194 (82.99%), Postives = 176/194 (90.72%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60
           M ASLLLRGHPL+R GL +RGLC+G+VQVTRRSCCTA  ASTPP G L+SAENT SV SA
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120
           +   KYQRW EP YRKWK+QE+EILSD++PV+SLTKEILHSNRYVDGERLT EDE+IVVD
Sbjct: 61  SQSTKYQRWCEPDYRKWKDQEDEILSDVEPVMSLTKEILHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFR+SRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRYSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PLYAERFIREHFKR 195
           P YAE FIREHFKR
Sbjct: 181 PSYAEMFIREHFKR 194

[Close]

BLAST of Carg10621 vs. TAIR 10
Match: AT3G46630.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 228.4 bits (581), Expect = 4.9e-60
Identity = 117/204 (57.35%), Postives = 145/204 (71.08%), Query Frame = 0


Query: 1   MVASLLLRGHPLIRLGLRNRG-----LCTGIVQVTRRSCCTA--MPASTPPGGGLSSAEN 60
           M + LLLR  PL+R G  N       +  GI+   RR  C+    P        +S  E 
Sbjct: 1   MTSLLLLRTLPLLRNGFLNHRHQVGIVAGGILSHRRRLLCSLADRPQFYENNDSVSPVEG 60

Query: 61  T-TSVLSATDPPKYQRW--DEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERL 120
           +  + ++ T P     W  ++P YRKWKN E EIL DI+P+  L KEILHS+RY+DGERL
Sbjct: 61  SGAAAMNVTSPVVDNSWRYEDPDYRKWKNLEAEILRDIEPISLLAKEILHSDRYLDGERL 120

Query: 121 TFEDEKIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQK 180
            FEDEKIV+++LL +HP+++DKIGCGL+ IMVDRHPQFRHSRCLFV+RTDGGWIDFSYQK
Sbjct: 121 DFEDEKIVMEKLLPYHPYSKDKIGCGLDFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQK 180

Query: 181 CLRAYIRNKYPLYAERFIREHFKR 195
           CLRAY+R+KYP +AERFIREHFKR
Sbjct: 181 CLRAYVRDKYPSHAERFIREHFKR 204

[Close]

BLAST of Carg10621 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 128.3 bits (321), Expect = 6.9e-30
Identity = 53/114 (46.49%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 81  EEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESI 140
           E++IL    P++   + ILHS +Y + +RL+ E E+ +++ LL +HP  E KIGCG++ I
Sbjct: 101 EDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYI 160

Query: 141 MVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYAERFIREHFKR 195
           MV  HP F  SRC+F++R DG  +DFSY KC++  I+ KYPLYA+ FI  HF++
Sbjct: 161 MVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of Carg10621 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 53/114 (46.49%), Postives = 79/114 (69.30%), Query Frame = 0


Query: 81  EEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCGLESI 140
           E++IL    P++   + ILHS +Y + +RL+ E E+ +++ LL +HP  E KIGCG++ I
Sbjct: 101 EDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYI 160

Query: 141 MVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPLYAERFIREHFKR 195
           MV  HP F  SRC+F++R DG  +DFSY KC++  I+ KYPLYA+ FI  HF++
Sbjct: 161 MVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRK 214

[Close]

BLAST of Carg10621 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 113.2 bits (282), Expect = 2.3e-25
Identity = 56/145 (38.62%), Postives = 90/145 (62.07%), Query Frame = 0


Query: 50   SAENTTSVLSATDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGER 109
            S +N   + +AT     QR D      + ++E+E+LSD++PV+   ++I+H + Y DG+ 
Sbjct: 1714 SEDNLAPMFTAT----RQRLD-----SFTSEEQELLSDVEPVMRTLRKIMHPSAYPDGDP 1773

Query: 110  LTFEDEKIVVDRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQ 169
            ++ +D+  V++++L  HP  E K+G G++ I VD+H  F  SRC FV+ TDG   DFSY+
Sbjct: 1774 ISDDDKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYR 1833

Query: 170  KCLRAYIRNKYPLYAERFIREHFKR 195
            K L  Y+  KYP  AE FI ++F +
Sbjct: 1834 KSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of Carg10621 vs. TAIR 10
Match: AT1G63020.1 (nuclear RNA polymerase D1A )

HSP 1 Score: 52.0 bits (123), Expect = 6.3e-07
Identity = 36/96 (37.50%), Postives = 50/96 (52.08%), Query Frame = 0


Query: 77   WKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVDRLLAHHPHAEDKIGCG 136
            WKN        I+ +    K ILHS  Y   E L   DE + V  +L  HP++ +KIG G
Sbjct: 1336 WKN--------IELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPG 1395

Query: 137  LESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCL 173
            ++ I V +  +   S C  V+R DG + DFSY KC+
Sbjct: 1396 VKGIRVAK-SKHGDSCCFEVVRIDGTFEDFSYHKCV 1419

The following BLAST results are available for this feature:

BLAST of Carg10621 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037785.1	3.1e-109	100.00	Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6608449.1	3.1e-109	100.00	Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022982288.1	5.9e-108	97.94	protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]
XP_023523235.1	1.3e-107	97.42	protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022940238.1	1.7e-107	97.94	protein DCL homolog, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of Carg10621 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C642	9.7e-29	46.49	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	2.2e-28	44.92	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q5D869	3.2e-24	38.62	DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1...	[more]
Q9LQ02	8.9e-06	37.50	DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]

BLAST of Carg10621 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J4G4	2.9e-108	97.94	protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1FNQ4	8.3e-108	97.94	protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1C4J4	4.3e-96	87.63	protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A1S3BU88	4.2e-91	81.44	protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1	[more]
A0A6J1IHJ1	4.6e-90	82.99	protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477...	[more]

BLAST of Carg10621 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46630.1	4.9e-60	57.35	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	6.9e-30	46.49	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	1.5e-29	46.49	Protein of unknown function (DUF3223)	[more]
AT2G40030.1	2.3e-25	38.62	nuclear RNA polymerase D1B	[more]
AT1G63020.1	6.3e-07	37.50	nuclear RNA polymerase D1A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.40		coord: 83..178 e-value: 2.3E-24 score: 87.7
None	No IPR available	PFAM	PF11523	DUF3223	coord: 96..172 e-value: 4.8E-28 score: 97.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..63
None	No IPR available	PANTHER	PTHR33415:SF4	DCL PROTEIN (DUF3223)	coord: 1..194
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 1..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10621-RA	Carg10621-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:1901259	chloroplast rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003729	mRNA binding