Carg10605 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10605
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cold regulated protein 27
Location	Carg_Chr01: 12358542 .. 12360497 (+)
RNA-Seq Expression	Carg10605
Synteny	Carg10605

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGATCTCTCTCCACTCGCCCGCTTCCATTTTTCTTCTCCATCTCTCTCTCTCTCGCCATTGCAAATCTGGAGATAATCCTTCATCTTCTTCTGTTTTCATGGAGCTTTTGCCTCCAAATTTAGAGAATCTCAAGCCTAAAGAAACTCGGACCGACTCACTGATCTCTGAATTGGCCATGGACGAGTCAACCGAGGCTCCCCTACAGGACGAATCTTCTAGCCAGGTACTTACCCTACTTTTCTTCTTCACGGTTCCAATTATCTTCGTTTTTGGATGTTTTTGTATTTCGGATTAGGTTTTGCTTATGTGATGTTTCGTATCTTCAATTTCATACTCTGTGCCCGTACTGTTTCTCTATTTTCGTGCTAAAATGAGAACGATATCGATTAAATAATTGAAAGAGAGAGGAAGTGGAAGAAGATAGTACTGGTCTGGCATTGACTCTCGAGACTGGAGAATTTTTATTTTGTATTTTGTTCGGCTCTGTTTAGATCGGTATTGCTGTTTGTCGATGGAATTTATTGGGCTGTGCAAGAGGTTTGGATCTGTGATCAATTATTCTGGAAACTCAGCACGAGCTTTGGTTGTCCGGATTTATTATTTATCATTATAATGACTAATAGTAGCGTTTTTCTGTTATTTATTAAGAGAGACCATGAACTTGCCGCGGGAATTGCTGATAATGCGGTTTCGATTTTAGGGCACTCGTATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATTTGTTCCTTGAATCCATGGAGGCATCTTTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGGTGGTACCGGCTCCGGCAAGGAAAATTCATCATGGACAAAATTGCACGGCCAAAGCCAGGCCGCTTCTCATGTCCACTCACATGTTGGCCAGGTTCAGTGCCGATTGTTTTTTCTTGATGAGTTTTAACTCGTTTCTGATCATGACTTGTGAGTGCTCTTTTTGTTATTTTGTTGCTTTCATCTACACAGTTTAAGGTTCTTCGACAAGGCAGCTGGAAGAATGTTAATTTTGAACCAACTGAAGCTCGGCCGAATTTATTGAATGATTACCAGGCTCTATCGCGCAATCCATGGATACATCACTTCAGAGCTGCTCGCAGAAACAAAAGTGTTGCATGTAAAAGTCCAGCCATCAGTTCAAGAAAAAGGAATTTGTTGCCATTTGGAGGAGATAATAATTCAGGACCTATGCGTGCTTGTAACTCTGATTTGTCTCAGCATTATATATACAACAATAAAGGTAGGTCTCTGGGCCTTTGTGCAGATAAATTATCAATATTGAATTGATAAATTGTGCCTCTCCTTGATTATAATTTAGTTATCACGTTTAAGTTTGCTAGTTAGTAATAATAAATTGGAATATGTTTGATCTGAATCTAGTCTTTACTGCCAGAAAGTTCTGAGTTCAAACAAATGATGCGACTGGAGTGAAATTGTTTTATCTATTGTAATCTGCTATGTTCAAGTTCCGACTATGATTTTAATTGCAATTTGTACTGCAATCATCATGTCGCCTGAATTTTAGATGAGAAAGATCCTTTATGTTGCAGAAGTGTCGGATCAGAACTTTGTTGATGGAGAAAGGGAAGTTGAAAAAGTTGCAAAGGGTAGCAATGATTGCAATACCAAAAGGGTAAAAACTTCAGAAACCAATGATTTGATCAACGACCAGGTAATTCAAATGATATCAACTTAGCTATCATGTCCTTGCTCCAAAATGACAACCACTGTTTTCTTTTAGGTTGTTCCTTAAGTTCCACGAAAAGGAGATGATGCTTAACAAGAATTCTCTTACAGCCAGCAGATAGCTAAATCCATGATTTTCGCCAAGACGGACTTGGTAGACTACTTTTCTAACCCGGGAAAGGCTATAGATGATCAGAGATTGTTGGTGAAGTCTGTAAAATCCTCATTTTGA

mRNA sequence

AAGATCTCTCTCCACTCGCCCGCTTCCATTTTTCTTCTCCATCTCTCTCTCTCTCGCCATTGCAAATCTGGAGATAATCCTTCATCTTCTTCTGTTTTCATGGAGCTTTTGCCTCCAAATTTAGAGAATCTCAAGCCTAAAGAAACTCGGACCGACTCACTGATCTCTGAATTGGCCATGGACGAGTCAACCGAGGCTCCCCTACAGGACGAATCTTCTAGCCAGGGCACTCGTATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATTTGTTCCTTGAATCCATGGAGGCATCTTTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGGTGGTACCGGCTCCGGCAAGGAAAATTCATCATGGACAAAATTGCACGGCCAAAGCCAGGCCGCTTCTCATGTCCACTCACATGTTGGCCAGTTTAAGGTTCTTCGACAAGGCAGCTGGAAGAATGTTAATTTTGAACCAACTGAAGCTCGGCCGAATTTATTGAATGATTACCAGGCTCTATCGCGCAATCCATGGATACATCACTTCAGAGCTGCTCGCAGAAACAAAAGTGTTGCATGTAAAAGTCCAGCCATCAGTTCAAGAAAAAGGAATTTGTTGCCATTTGGAGGAGATAATAATTCAGGACCTATGCGTGCTTGTAACTCTGATTTGTCTCAGCATTATATATACAACAATAAAGAAGTGTCGGATCAGAACTTTGTTGATGGAGAAAGGGAAGTTGAAAAAGTTGCAAAGGGTAGCAATGATTGCAATACCAAAAGGGTAAAAACTTCAGAAACCAATGATTTGATCAACGACCAGATAGCTAAATCCATGATTTTCGCCAAGACGGACTTGGTAGACTACTTTTCTAACCCGGGAAAGGCTATAGATGATCAGAGATTGTTGGTGAAGTCTGTAAAATCCTCATTTTGA

Coding sequence (CDS)

ATGGAGCTTTTGCCTCCAAATTTAGAGAATCTCAAGCCTAAAGAAACTCGGACCGACTCACTGATCTCTGAATTGGCCATGGACGAGTCAACCGAGGCTCCCCTACAGGACGAATCTTCTAGCCAGGGCACTCGTATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATTTGTTCCTTGAATCCATGGAGGCATCTTTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGGTGGTACCGGCTCCGGCAAGGAAAATTCATCATGGACAAAATTGCACGGCCAAAGCCAGGCCGCTTCTCATGTCCACTCACATGTTGGCCAGTTTAAGGTTCTTCGACAAGGCAGCTGGAAGAATGTTAATTTTGAACCAACTGAAGCTCGGCCGAATTTATTGAATGATTACCAGGCTCTATCGCGCAATCCATGGATACATCACTTCAGAGCTGCTCGCAGAAACAAAAGTGTTGCATGTAAAAGTCCAGCCATCAGTTCAAGAAAAAGGAATTTGTTGCCATTTGGAGGAGATAATAATTCAGGACCTATGCGTGCTTGTAACTCTGATTTGTCTCAGCATTATATATACAACAATAAAGAAGTGTCGGATCAGAACTTTGTTGATGGAGAAAGGGAAGTTGAAAAAGTTGCAAAGGGTAGCAATGATTGCAATACCAAAAGGGTAAAAACTTCAGAAACCAATGATTTGATCAACGACCAGATAGCTAAATCCATGATTTTCGCCAAGACGGACTTGGTAGACTACTTTTCTAACCCGGGAAAGGCTATAGATGATCAGAGATTGTTGGTGAAGTCTGTAAAATCCTCATTTTGA

Protein sequence

MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQIAKSMIFAKTDLVDYFSNPGKAIDDQRLLVKSVKSSF

Homology

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BLAST of Carg10605 vs. NCBI nr
Match: KAG7037801.1 (hypothetical protein SDJN02_01432 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 553.5 bits (1425), Expect = 1.0e-153
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN
Sbjct: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
           NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240

Query: 241 DQIAKSMIFAKTDLVDYFSNPGKAIDDQRLLVKSVKSSF 280
           DQIAKSMIFAKTDLVDYFSNPGKAIDDQRLLVKSVKSSF
Sbjct: 241 DQIAKSMIFAKTDLVDYFSNPGKAIDDQRLLVKSVKSSF 279

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BLAST of Carg10605 vs. NCBI nr
Match: KAG6608473.1 (Cold-regulated protein 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 482.6 bits (1241), Expect = 2.2e-132
Identity = 241/243 (99.18%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNK+VACKSPAISSRKRNLLPFGGDN
Sbjct: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDN 232

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
           NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 233 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292

Query: 241 DQI 244
           DQ+
Sbjct: 293 DQV 295

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BLAST of Carg10605 vs. NCBI nr
Match: XP_022982275.1 (uncharacterized protein LOC111481154 [Cucurbita maxima])

HSP 1 Score: 478.8 bits (1231), Expect = 3.2e-131
Identity = 238/243 (97.94%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NK+VACKSPAISSRKRNLLPFGGDN
Sbjct: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDN 232

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
           NSGPMRACNSDLS+HYIYNNKE+SDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 233 NSGPMRACNSDLSRHYIYNNKEMSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292

Query: 241 DQI 244
           DQ+
Sbjct: 293 DQV 295

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BLAST of Carg10605 vs. NCBI nr
Match: XP_023525276.1 (uncharacterized protein LOC111788927 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476.1 bits (1224), Expect = 2.1e-130
Identity = 237/243 (97.53%), Postives = 242/243 (99.59%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           MELLPPNLENLKPKETRTDSLIS LAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MELLPPNLENLKPKETRTDSLISVLAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KNVNFEPTE+RPNLLNDY+ALSRNPWIHHFRAAR+NK+VACKSPAISSRKRNLLPFGGDN
Sbjct: 121 KNVNFEPTESRPNLLNDYRALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDN 180

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
           NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240

Query: 241 DQI 244
           DQ+
Sbjct: 241 DQV 243

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BLAST of Carg10605 vs. NCBI nr
Match: XP_022940327.1 (uncharacterized protein LOC111445977 isoform X1 [Cucurbita moschata])

HSP 1 Score: 461.5 bits (1186), Expect = 5.3e-126
Identity = 233/243 (95.88%), Postives = 238/243 (97.94%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           ME+LPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MEVLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NK+VACKSPAISSRKRNLLPFG   
Sbjct: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFG--- 180

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
            +GPMRACNSDLSQH IYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 -AGPMRACNSDLSQHCIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 239

Query: 241 DQI 244
           DQ+
Sbjct: 241 DQV 239

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BLAST of Carg10605 vs. ExPASy Swiss-Prot
Match: Q8L8T7 (Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.3e-10
Identity = 63/204 (30.88%), Postives = 103/204 (50.49%), Query Frame = 0


Query: 37  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 96
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 97  GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RRNKSVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 216
            + +      P     + S+ K+ +   G  ++   + + + D  Q  +    EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISV-GEAEVSDQNF 210

Query: 217 VDGEREVEKVAKGSNDCNTKRVKT 233
           V+      +  KG N  ++K++KT
Sbjct: 211 VN------EGIKGENG-SSKKMKT 217

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BLAST of Carg10605 vs. ExPASy Swiss-Prot
Match: Q8L983 (Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 8.3e-05
Identity = 59/233 (25.32%), Postives = 91/233 (39.06%), Query Frame = 0


Query: 19  DSLISELAMDESTEAPLQDESSSQGTRMTESVPSD--------WTDEKHHLFLESMEASF 78
           D  +SE + +  +E+ L +   S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 79  VSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEA 138
           V Q++ S   GGT          +L       S+ H    QF VL+ G W+ VNF   ++
Sbjct: 75  VRQLY-SLLGGGT---------QRLSRTRDVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 139 RPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDNNSGPMRACNS 198
                     L  +      R + RNK                 P   + N         
Sbjct: 135 ---------CLETSSEFRFHRNSLRNK-----------------PENSNGNYTMGTTVQG 194

Query: 199 DLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQI 244
           D+  H    + E S QNF + E E E   KG      +R   ++ + L  DQ+
Sbjct: 195 DVLCHDETKHSEASGQNFREEEEEEE---KGEVSKKREREANNDDSSLKEDQV 208

[Close]

BLAST of Carg10605 vs. ExPASy TrEMBL
Match: A0A6J1J278 (uncharacterized protein LOC111481154 OS=Cucurbita maxima OX=3661 GN=LOC111481154 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.6e-131
Identity = 238/243 (97.94%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NK+VACKSPAISSRKRNLLPFGGDN
Sbjct: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDN 232

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
           NSGPMRACNSDLS+HYIYNNKE+SDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 233 NSGPMRACNSDLSRHYIYNNKEMSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292

Query: 241 DQI 244
           DQ+
Sbjct: 293 DQV 295

[Close]

BLAST of Carg10605 vs. ExPASy TrEMBL
Match: A0A6J1FJA4 (uncharacterized protein LOC111445977 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445977 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.6e-126
Identity = 233/243 (95.88%), Postives = 238/243 (97.94%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           ME+LPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH
Sbjct: 1   MEVLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NK+VACKSPAISSRKRNLLPFG   
Sbjct: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFG--- 180

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
            +GPMRACNSDLSQH IYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN
Sbjct: 181 -AGPMRACNSDLSQHCIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 239

Query: 241 DQI 244
           DQ+
Sbjct: 241 DQV 239

[Close]

BLAST of Carg10605 vs. ExPASy TrEMBL
Match: A0A6J1FNZ0 (uncharacterized protein LOC111445977 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445977 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 4.4e-102
Identity = 189/198 (95.45%), Postives = 193/198 (97.47%), Query Frame = 0


Query: 46  MTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHV 105
           MTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHV
Sbjct: 1   MTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHV 60

Query: 106 HSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPA 165
           HSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAAR+NK+VACKSPA
Sbjct: 61  HSHVGQFKVLRQGSWKNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPA 120

Query: 166 ISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDC 225
           ISSRKRNLLPFG    +GPMRACNSDLSQH IYNNKEVSDQNFVDGEREVEKVAKGSNDC
Sbjct: 121 ISSRKRNLLPFG----AGPMRACNSDLSQHCIYNNKEVSDQNFVDGEREVEKVAKGSNDC 180

Query: 226 NTKRVKTSETNDLINDQI 244
           NTKRVKTSETNDLINDQ+
Sbjct: 181 NTKRVKTSETNDLINDQV 194

[Close]

BLAST of Carg10605 vs. ExPASy TrEMBL
Match: A0A6J1F6L8 (uncharacterized protein LOC111442637 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442637 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 6.0e-91
Identity = 188/259 (72.59%), Postives = 206/259 (79.54%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           MELLPPNLEN    ETRTDSLISELA DESTE  L  ESSS GT MT+SVPSDWTDEKHH
Sbjct: 1   MELLPPNLENHNAVETRTDSLISELAKDESTEFALPVESSSLGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESME SFVSQMFDS HS G+   KENSS TKLHGQSQ+ASHVHSH GQFKVLR+GSW
Sbjct: 61  LFLESMETSFVSQMFDSVHSVGSCPRKENSSHTKLHGQSQSASHVHSHFGQFKVLRRGSW 120

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KN+NFE TE+R NLLND+QALSRNPWIHHFRA R+NK+VACKS AI S  RNL P G  N
Sbjct: 121 KNINFETTESRSNLLNDFQALSRNPWIHHFRADRKNKNVACKSQAIGSGGRNLFPLGAAN 180

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDC-NTKRVKTSETNDLI 240
           +S P+RAC+SDLS  YI +N+EVSDQNFVD +RE   V  GS+DC N KRV T ETN LI
Sbjct: 181 DSEPLRACDSDLSLQYISSNEEVSDQNFVDEDRE---VGNGSSDCSNAKRVNTPETNALI 240

Query: 241 NDQ-IAKSMIFAKTDLVDY 258
           N+Q IA+S I A T    Y
Sbjct: 241 NEQKIARSQIVANTRQTTY 256

[Close]

BLAST of Carg10605 vs. ExPASy TrEMBL
Match: A0A6J1IFL9 (uncharacterized protein LOC111476815 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476815 PE=4 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 7.8e-91
Identity = 185/258 (71.71%), Postives = 205/258 (79.46%), Query Frame = 0


Query: 1   MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60
           MELLPPNLEN    ETRTD LISEL  D+STE  L  ESSS GT MT+SVPSDWTDEKHH
Sbjct: 1   MELLPPNLENHIAVETRTDLLISELPKDDSTEFALPVESSSLGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120
           LFLESME SFVSQMFDS HS G+   KENSS TKLHGQSQ+ASHVH+H GQFKVLR+GSW
Sbjct: 61  LFLESMETSFVSQMFDSVHSVGSCPRKENSSHTKLHGQSQSASHVHAHFGQFKVLRRGSW 120

Query: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDN 180
           KN+NFE TE+R NLLND+QALSRNPWIHHFRA R+NK+VACKS AI S  RNL P G  N
Sbjct: 121 KNINFETTESRSNLLNDFQALSRNPWIHHFRADRKNKNVACKSQAIGSGGRNLFPLGAAN 180

Query: 181 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 240
           NS P+RAC SDLS  YI +N+EVSDQNFVD +RE   V  GS+DCN KRV T ETN LIN
Sbjct: 181 NSEPLRACGSDLSLQYISSNEEVSDQNFVDEDRE---VGNGSSDCNAKRVNTPETNALIN 240

Query: 241 DQ-IAKSMIFAKTDLVDY 258
           +Q IA+S+I A T  + Y
Sbjct: 241 EQKIARSLIVANTRQMTY 255

[Close]

BLAST of Carg10605 vs. TAIR 10
Match: AT5G42900.3 (cold regulated gene 27 )

HSP 1 Score: 69.3 bits (168), Expect = 5.5e-12
Identity = 64/204 (31.37%), Postives = 104/204 (50.98%), Query Frame = 0


Query: 37  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 96
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 97  GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RRNKSVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 216
            + +      P     + S+ K+ +   G  ++   + + + D  Q  I   +EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQ--ISVGEEVSDQNF 210

Query: 217 VDGEREVEKVAKGSNDCNTKRVKT 233
           V+      +  KG N  ++K++KT
Sbjct: 211 VN------EGIKGENG-SSKKMKT 216

[Close]

BLAST of Carg10605 vs. TAIR 10
Match: AT5G42900.1 (cold regulated gene 27 )

HSP 1 Score: 68.6 bits (166), Expect = 9.3e-12
Identity = 63/204 (30.88%), Postives = 103/204 (50.49%), Query Frame = 0


Query: 37  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 96
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 97  GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RRNKSVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 216
            + +      P     + S+ K+ +   G  ++   + + + D  Q  +    EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISV-GEAEVSDQNF 210

Query: 217 VDGEREVEKVAKGSNDCNTKRVKT 233
           V+      +  KG N  ++K++KT
Sbjct: 211 VN------EGIKGENG-SSKKMKT 217

[Close]

BLAST of Carg10605 vs. TAIR 10
Match: AT5G42900.2 (cold regulated gene 27 )

HSP 1 Score: 68.6 bits (166), Expect = 9.3e-12
Identity = 63/204 (30.88%), Postives = 103/204 (50.49%), Query Frame = 0


Query: 37  DESSSQGTRMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLH 96
           + +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S    G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSL---GALGKNENVSESTRF 90

Query: 97  GQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEARPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ +N +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RRNKSVACKSP-----AISSRKRNLLPFGGDNNSGPMRACNSDLSQHYIYNNKEVSDQNF 216
            + +      P     + S+ K+ +   G  ++   + + + D  Q  +    EVSDQNF
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISV-GEAEVSDQNF 210

Query: 217 VDGEREVEKVAKGSNDCNTKRVKT 233
           V+      +  KG N  ++K++KT
Sbjct: 211 VN------EGIKGENG-SSKKMKT 217

[Close]

BLAST of Carg10605 vs. TAIR 10
Match: AT4G33980.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.3). )

HSP 1 Score: 52.8 bits (125), Expect = 5.3e-07
Identity = 64/253 (25.30%), Postives = 100/253 (39.53%), Query Frame = 0


Query: 19  DSLISELAMDESTEAPLQDESSSQGTRMTESVPSD--------WTDEKHHLFLESMEASF 78
           D  +SE + +  +E+ L +   S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 79  VSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEA 138
           V Q++ S   GGT          +L       S+ H    QF VL+ G W+ VNF   ++
Sbjct: 75  VRQLY-SLLGGGT---------QRLSRTRDVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 139 RPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDNNSGPMRACNS 198
                     L  +      R + RNK                 P   + N         
Sbjct: 135 ---------CLETSSEFRFHRNSLRNK-----------------PENSNGNYTMGTTVQG 194

Query: 199 DLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQIAK-SMIF 258
           D+  H    + E S QNF + E E E   KG      +R   ++ + L  DQ+    M+ 
Sbjct: 195 DVLCHDETKHSEASGQNFREEEEEEE---KGEVSKKREREANNDDSSLKEDQVVPVRMVK 228

Query: 259 AKTDLVDYFSNPG 263
            +TD + Y +  G
Sbjct: 255 PRTDPMGYGNRIG 228

[Close]

BLAST of Carg10605 vs. TAIR 10
Match: AT4G33980.1 (BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 49.3 bits (116), Expect = 5.9e-06
Identity = 59/233 (25.32%), Postives = 91/233 (39.06%), Query Frame = 0


Query: 19  DSLISELAMDESTEAPLQDESSSQGTRMTESVPSD--------WTDEKHHLFLESMEASF 78
           D  +SE + +  +E+ L +   S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 79  VSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSWKNVNFEPTEA 138
           V Q++ S   GGT          +L       S+ H    QF VL+ G W+ VNF   ++
Sbjct: 75  VRQLY-SLLGGGT---------QRLSRTRDVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 139 RPNLLNDYQALSRNPWIHHFRAARRNKSVACKSPAISSRKRNLLPFGGDNNSGPMRACNS 198
                     L  +      R + RNK                 P   + N         
Sbjct: 135 ---------CLETSSEFRFHRNSLRNK-----------------PENSNGNYTMGTTVQG 194

Query: 199 DLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLINDQI 244
           D+  H    + E S QNF + E E E   KG      +R   ++ + L  DQ+
Sbjct: 195 DVLCHDETKHSEASGQNFREEEEEEE---KGEVSKKREREANNDDSSLKEDQV 208

The following BLAST results are available for this feature:

BLAST of Carg10605 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037801.1	1.0e-153	100.00	hypothetical protein SDJN02_01432 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6608473.1	2.2e-132	99.18	Cold-regulated protein 27, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022982275.1	3.2e-131	97.94	uncharacterized protein LOC111481154 [Cucurbita maxima]	[more]
XP_023525276.1	2.1e-130	97.53	uncharacterized protein LOC111788927 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022940327.1	5.3e-126	95.88	uncharacterized protein LOC111445977 isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg10605 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L8T7	1.3e-10	30.88	Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1	[more]
Q8L983	8.3e-05	25.32	Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1	[more]

BLAST of Carg10605 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J278	1.6e-131	97.94	uncharacterized protein LOC111481154 OS=Cucurbita maxima OX=3661 GN=LOC111481154...	[more]
A0A6J1FJA4	2.6e-126	95.88	uncharacterized protein LOC111445977 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1FNZ0	4.4e-102	95.45	uncharacterized protein LOC111445977 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1F6L8	6.0e-91	72.59	uncharacterized protein LOC111442637 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IFL9	7.8e-91	71.71	uncharacterized protein LOC111476815 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Carg10605 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42900.3	5.5e-12	31.37	cold regulated gene 27	[more]
AT5G42900.1	9.3e-12	30.88	cold regulated gene 27	[more]
AT5G42900.2	9.3e-12	30.88	cold regulated gene 27	[more]
AT4G33980.2	5.3e-07	25.30	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOC...	[more]
AT4G33980.1	5.9e-06	25.32	BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G429...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..50
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..53
None	No IPR available	PANTHER	PTHR33676:SF3	COLD REGULATED PROTEIN 27	coord: 17..244
IPR044678	Cold-regulated protein 27/28	PANTHER	PTHR33676	COLD REGULATED PROTEIN 27	coord: 17..244

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10605-RA	Carg10605-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042752	regulation of circadian rhythm
biological_process	GO:0009409	response to cold