Carg10495 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10495
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCasparian strip membrane protein 1
LocationCarg_Chr03: 8494848 .. 8495983 (-)
RNA-Seq ExpressionCarg10495
SyntenyCarg10495
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAACGCCGAATCCTCTGCCATCAATGTTGCCTCCGAGACCACCAACAGCGGCCCAATTGGGAAGAAAAAGAGCACGACCCCGTTGCTAGCGGCGGAGGTCCCAATCCGGCCAGACAGGGGAACCCACCGGATGAAACGTGGGCTTGCCATTTTCGACATCGTTCTGAGAATCGGCGCCCTTTCCTCGGCGCTGGCGGCGGCAGCCACCATGGGAACCAGTGGCCAAACGTTGCCGTTTTTCACTCAGTTCTTCCAGTTCGAGGCTAGCTACGACGATTTGCCCACTTTCCAGTTGGGTGGTTTTCATTCTTTTTTAAAAATACTCTTCTTATTGCGATTGAATTACTTAAACAAATAATTGAATTTTTTAATTGAAATTTGCAGGTTTTTTGTGATCGTAATGGGAATCGTTGCGGGATATGTGGTTCTATCACTTCCATTCTCAATTGTTTGCATTATTCGTCCCCATGCTGCTGGGCCTCGCCTTCTCCTCTTGATTTTGGACTCCGTAAGTATTCACTTAAAACTCTCGAAACTACGACTCTCCACGATAGTATGATATTGTCCACTTTGATGCTTTGAGCTCCCTCAAAAAACCTCATACGATCCTCAAAAATGACTGTTAGGAACCACGACTATCTACAATTGTATGATATTATCTATTTTGAGTATAAGCTCTCGTGATTTTGTTTCGAGCTTTCCCAATGTTAAGAACCATGACTCTCTACAATGATTTGATATTCTTAGGAACCACGACTCTTCGCAGTAGCATGATGTTGTTCACTTTGAGAATAAGCTCTCGTGACTACATTGGGCTTCTCCCAAAGACCTCATAAGACCTCGTAGCTTACTTATACACCCATGATCATTTTCTAGGACTCCATTAAAGAAAATATATGTCGTGGTTTGCAGGTGGCTTTGGCGTTGAGCACAGCGGCAGCAGGAGCGGCGGCCGCAGTGGTGGCGCTTGCACACAGTGGGAACTCAAGCACGAACTGGCTAGCAGTGTGCAACCAATTTGGGGACTTTTGCCAACAAGCGAGTGGGGCTGTGGTGGGCTCTTTTGCTGCTGTTTTGCTCTTCCTCCTACTCATCTTGTTCTCTGCCTTGTCCCTCAAGAGTGCCCATTGA

mRNA sequence

ATGAAGAACGCCGAATCCTCTGCCATCAATGTTGCCTCCGAGACCACCAACAGCGGCCCAATTGGGAAGAAAAAGAGCACGACCCCGTTGCTAGCGGCGGAGGTCCCAATCCGGCCAGACAGGGGAACCCACCGGATGAAACGTGGGCTTGCCATTTTCGACATCGTTCTGAGAATCGGCGCCCTTTCCTCGGCGCTGGCGGCGGCAGCCACCATGGGAACCAGTGGCCAAACGTTGCCGTTTTTCACTCAGTTCTTCCAGTTCGAGGCTAGCTACGACGATTTGCCCACTTTCCAGTTTTTTGTGATCGTAATGGGAATCGTTGCGGGATATGTGGTTCTATCACTTCCATTCTCAATTGTTTGCATTATTCGTCCCCATGCTGCTGGGCCTCGCCTTCTCCTCTTGATTTTGGACTCCGTGGCTTTGGCGTTGAGCACAGCGGCAGCAGGAGCGGCGGCCGCAGTGGTGGCGCTTGCACACAGTGGGAACTCAAGCACGAACTGGCTAGCAGTGTGCAACCAATTTGGGGACTTTTGCCAACAAGCGAGTGGGGCTGTGGTGGGCTCTTTTGCTGCTGTTTTGCTCTTCCTCCTACTCATCTTGTTCTCTGCCTTGTCCCTCAAGAGTGCCCATTGA

Coding sequence (CDS)

ATGAAGAACGCCGAATCCTCTGCCATCAATGTTGCCTCCGAGACCACCAACAGCGGCCCAATTGGGAAGAAAAAGAGCACGACCCCGTTGCTAGCGGCGGAGGTCCCAATCCGGCCAGACAGGGGAACCCACCGGATGAAACGTGGGCTTGCCATTTTCGACATCGTTCTGAGAATCGGCGCCCTTTCCTCGGCGCTGGCGGCGGCAGCCACCATGGGAACCAGTGGCCAAACGTTGCCGTTTTTCACTCAGTTCTTCCAGTTCGAGGCTAGCTACGACGATTTGCCCACTTTCCAGTTTTTTGTGATCGTAATGGGAATCGTTGCGGGATATGTGGTTCTATCACTTCCATTCTCAATTGTTTGCATTATTCGTCCCCATGCTGCTGGGCCTCGCCTTCTCCTCTTGATTTTGGACTCCGTGGCTTTGGCGTTGAGCACAGCGGCAGCAGGAGCGGCGGCCGCAGTGGTGGCGCTTGCACACAGTGGGAACTCAAGCACGAACTGGCTAGCAGTGTGCAACCAATTTGGGGACTTTTGCCAACAAGCGAGTGGGGCTGTGGTGGGCTCTTTTGCTGCTGTTTTGCTCTTCCTCCTACTCATCTTGTTCTCTGCCTTGTCCCTCAAGAGTGCCCATTGA

Protein sequence

MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLKSAH
Homology
BLAST of Carg10495 vs. NCBI nr
Match: KAG7034486.1 (hypothetical protein SDJN02_04216, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384.4 bits (986), Expect = 6.3e-103
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLKSAH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 212

BLAST of Carg10495 vs. NCBI nr
Match: XP_023544952.1 (casparian strip membrane protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 383.3 bits (983), Expect = 1.4e-102
Identity = 211/212 (99.53%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495 vs. NCBI nr
Match: XP_022977901.1 (casparian strip membrane protein 1 [Cucurbita maxima])

HSP 1 Score: 382.5 bits (981), Expect = 2.4e-102
Identity = 210/212 (99.06%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLS+PFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495 vs. NCBI nr
Match: KAG6604335.1 (hypothetical protein SDJN03_04944, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 381.7 bits (979), Expect = 4.1e-102
Identity = 210/212 (99.06%), Postives = 211/212 (99.53%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+ H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNGH 212

BLAST of Carg10495 vs. NCBI nr
Match: XP_022926164.1 (casparian strip membrane protein 1-like [Cucurbita moschata])

HSP 1 Score: 380.2 bits (975), Expect = 1.2e-101
Identity = 210/212 (99.06%), Postives = 211/212 (99.53%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSG IGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGSIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495 vs. ExPASy Swiss-Prot
Match: P0DI61 (Casparian strip membrane protein 1 OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 1.5e-91
Identity = 184/212 (86.79%), Postives = 197/212 (92.92%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK  ES+AI++A ET NSGPIGKKKSTTPLLAA V   PDRGTHRMKRGLAIFD VLRIG
Sbjct: 1   MKTGESTAIDIAPETNNSGPIGKKKSTTPLLAAPV---PDRGTHRMKRGLAIFDFVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
            L+SALAAAA MGTS QTLPFFTQFFQFEASYDDLPTFQFFV+ M +VAGYVVLS+PFSI
Sbjct: 61  VLASALAAAAAMGTSEQTLPFFTQFFQFEASYDDLPTFQFFVVAMAVVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPR+LLLILDSVAL L+TAAAGAAAAVV+LAHSGNSSTNWLA+CNQFGDFC
Sbjct: 121 VCIIRPHAAGPRVLLLILDSVALTLNTAAAGAAAAVVSLAHSGNSSTNWLAICNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK++H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNSH 209

BLAST of Carg10495 vs. ExPASy Swiss-Prot
Match: B9IIR5 (Casparian strip membrane protein 2 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_576556 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.8e-60
Identity = 127/185 (68.65%), Postives = 156/185 (84.32%), Query Frame = 0

Query: 25  KSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQ 84
           K T PL+AA  P++   G    K+G+AIFD +LR+ A+++ALAAAA+MGTS +TLPFFTQ
Sbjct: 18  KGTAPLIAA--PMKEKGG---YKKGIAIFDFILRLAAIATALAAAASMGTSDETLPFFTQ 77

Query: 85  FFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIRPHAAGPRLLLLILDSVALA 144
           FFQF+ASYDDLPTFQFFVI M IVAGY+VLSLPFSIV I+RPHAAGPRLLL+ILD+VAL 
Sbjct: 78  FFQFQASYDDLPTFQFFVIAMAIVAGYLVLSLPFSIVAIVRPHAAGPRLLLIILDTVALT 137

Query: 145 LSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFS 204
           L+TAA  AAAA+V LAH+GNSSTNWLA+C QFGDFCQ+ SGAVV SF  V++F+ L++ S
Sbjct: 138 LNTAAGAAAAAIVYLAHNGNSSTNWLAICQQFGDFCQKNSGAVVASFITVVIFVFLLVLS 197

Query: 205 ALSLK 210
           A +L+
Sbjct: 198 AFALR 197

BLAST of Carg10495 vs. ExPASy Swiss-Prot
Match: B9IIR4 (Casparian strip membrane protein 3 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_907513 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.4e-59
Identity = 130/204 (63.73%), Postives = 161/204 (78.92%), Query Frame = 0

Query: 6   SSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSA 65
           S+ +++ ++T+ +      K T PL+AA   ++        K+GLAIFD+VLR+GA+ +A
Sbjct: 2   SARVDIPADTSAAA-----KGTAPLIAASTHVKGG-----YKKGLAIFDLVLRLGAVVTA 61

Query: 66  LAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIR 125
           LAAAATMGT+ QTLPFFTQFFQF+ASYDDLPTFQFFVI M IV+GY+VLSLPFSIV IIR
Sbjct: 62  LAAAATMGTTDQTLPFFTQFFQFQASYDDLPTFQFFVIAMAIVSGYLVLSLPFSIVAIIR 121

Query: 126 PHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQQASG 185
           PHA GPRLLL+ILD+VAL L+TAAA AA A+V LA +GNSS NWL +C QFGDFCQ+ASG
Sbjct: 122 PHATGPRLLLIILDTVALTLNTAAAAAAVAIVDLAQNGNSSANWLGICQQFGDFCQKASG 181

Query: 186 AVVGSFAAVLLFLLLILFSALSLK 210
           AVV SF A  + L LI+ SAL+L+
Sbjct: 182 AVVASFIAAGVLLFLIVISALALR 195

BLAST of Carg10495 vs. ExPASy Swiss-Prot
Match: B9SX12 (Casparian strip membrane protein 1 OS=Ricinus communis OX=3988 GN=RCOM_1259250 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 9.9e-59
Identity = 130/204 (63.73%), Postives = 159/204 (77.94%), Query Frame = 0

Query: 6   SSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALSSA 65
           S+ I + +E   S  + K K+  PL+ A       +G +  K+G+AIFD +LR+GA+ SA
Sbjct: 2   STTIEIPAE---SSAVAKGKA--PLIGASSSSYEKKGGY--KKGIAIFDFILRLGAVISA 61

Query: 66  LAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCIIR 125
           L+AAATMGTS +TLPFFTQFFQFEA YDD PTFQFFVI MG V GY+VLSLPFS+V IIR
Sbjct: 62  LSAAATMGTSDETLPFFTQFFQFEAGYDDFPTFQFFVIAMGFVGGYLVLSLPFSVVAIIR 121

Query: 126 PHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQQASG 185
           PHA G RLLLLILD+VAL L+TAAA AAAA+V LAH+GN S NWLAVC QFGDFCQ+ SG
Sbjct: 122 PHAVGIRLLLLILDTVALTLNTAAAAAAAAIVYLAHNGNQSANWLAVCQQFGDFCQKVSG 181

Query: 186 AVVGSFAAVLLFLLLILFSALSLK 210
            VV SF +VL+FLLL++ SA++L+
Sbjct: 182 GVVASFVSVLVFLLLVVMSAVALR 198

BLAST of Carg10495 vs. ExPASy Swiss-Prot
Match: B9HBX2 (Casparian strip membrane protein 1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_763057 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 8.3e-58
Identity = 125/206 (60.68%), Postives = 163/206 (79.13%), Query Frame = 0

Query: 4   AESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALS 63
           +ES+AI++   ++ +      K   PL+A     R ++G +R  +G+AIFD +LR+ A++
Sbjct: 3   SESAAIDIPESSSVA------KGKAPLIAVS---RNEKGGYR--KGIAIFDFILRLAAIA 62

Query: 64  SALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCI 123
           +ALAAAA MGTS +TLPFFTQFFQF+ASYDDLPTFQFFVI + IV GY+VLSLPFSIV I
Sbjct: 63  TALAAAAAMGTSDETLPFFTQFFQFQASYDDLPTFQFFVIAIAIVGGYLVLSLPFSIVAI 122

Query: 124 IRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQQA 183
           +RPHA GPRLLL+ILD+VAL L+TAA  AAAA+V LAH+GNS+TNWLA+C Q+GDFCQ+ 
Sbjct: 123 VRPHAVGPRLLLIILDAVALTLNTAAGAAAAAIVYLAHNGNSNTNWLAICQQYGDFCQKV 182

Query: 184 SGAVVGSFAAVLLFLLLILFSALSLK 210
           SGAVV SF  V++F+ LI+ SA +L+
Sbjct: 183 SGAVVASFITVVIFVFLIVLSAFALR 197

BLAST of Carg10495 vs. ExPASy TrEMBL
Match: A0A6J1IL91 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111478054 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.2e-102
Identity = 210/212 (99.06%), Postives = 212/212 (100.00%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLS+PFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495 vs. ExPASy TrEMBL
Match: A0A6J1EEC6 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433348 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.7e-102
Identity = 210/212 (99.06%), Postives = 211/212 (99.53%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKNAESSAINVASETTNSG IGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG
Sbjct: 1   MKNAESSAINVASETTNSGSIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI
Sbjct: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK+AH
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNAH 212

BLAST of Carg10495 vs. ExPASy TrEMBL
Match: A0A6J1BV79 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111005840 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.1e-89
Identity = 183/208 (87.98%), Postives = 196/208 (94.23%), Query Frame = 0

Query: 2   KNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGA 61
           KN+ES+AI+VA E  NSGPIGKKKSTTPLLAA VP+R DRGTHRMKRGLAIFD VLRIGA
Sbjct: 3   KNSESTAIDVAPENPNSGPIGKKKSTTPLLAAPVPVRQDRGTHRMKRGLAIFDFVLRIGA 62

Query: 62  LSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIV 121
           L++ALAAAA MGTS +TLPFFTQFFQFEASYDDLPTFQFFVI MGIVAGYVVLS+PFSIV
Sbjct: 63  LAAALAAAAAMGTSDETLPFFTQFFQFEASYDDLPTFQFFVIAMGIVAGYVVLSIPFSIV 122

Query: 122 CIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQ 181
           CI+RPHA GPRLLLLILDSVAL L+T AAGAAAAVV+LAHSGNSSTNWLA+CNQFGDFCQ
Sbjct: 123 CIVRPHAVGPRLLLLILDSVALTLTTVAAGAAAAVVSLAHSGNSSTNWLAICNQFGDFCQ 182

Query: 182 QASGAVVGSFAAVLLFLLLILFSALSLK 210
           QASGAV+GSFAAVLLFLLLILFSALSLK
Sbjct: 183 QASGAVIGSFAAVLLFLLLILFSALSLK 210

BLAST of Carg10495 vs. ExPASy TrEMBL
Match: A0A0A0KKJ8 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G453800 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.5e-89
Identity = 184/212 (86.79%), Postives = 199/212 (93.87%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK  ES++I++A ET NSGPIGKKKSTTPLLAA VP+  DRGTHRMKRGL+IFD VLRIG
Sbjct: 1   MKTGESTSIDIAPETNNSGPIGKKKSTTPLLAAPVPV-TDRGTHRMKRGLSIFDFVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
            L+SALAAAATMGTS QTLPFFTQFFQFEASYDDLPTFQFFV+ M +VAGYVVLS+PFSI
Sbjct: 61  VLASALAAAATMGTSDQTLPFFTQFFQFEASYDDLPTFQFFVVAMAVVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPR+LLLILDSVAL L+TAAAGAAAAVV+LAHSGNSSTNWLAVCNQFGDFC
Sbjct: 121 VCIIRPHAAGPRVLLLILDSVALTLNTAAAGAAAAVVSLAHSGNSSTNWLAVCNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK++H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNSH 211

BLAST of Carg10495 vs. ExPASy TrEMBL
Match: A0A5A7SKE2 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00860 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 5.6e-89
Identity = 184/212 (86.79%), Postives = 197/212 (92.92%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MK  ES+AI++A ET NSGPIGKKKSTTPLLAA V   PDRGTHRMKRGLAIFD VLRIG
Sbjct: 1   MKTGESTAIDIAPETNNSGPIGKKKSTTPLLAAPV---PDRGTHRMKRGLAIFDFVLRIG 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
            L+SALAAAA MGTS QTLPFFTQFFQFEASYDDLPTFQFFV+ M +VAGYVVLS+PFSI
Sbjct: 61  VLASALAAAAAMGTSEQTLPFFTQFFQFEASYDDLPTFQFFVVAMAVVAGYVVLSIPFSI 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           VCIIRPHAAGPR+LLLILDSVAL L+TAAAGAAAAVV+LAHSGNSSTNWLA+CNQFGDFC
Sbjct: 121 VCIIRPHAAGPRVLLLILDSVALTLNTAAAGAAAAVVSLAHSGNSSTNWLAICNQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
           QQASGAVVGSFAAVLLFLLLILFSALSLK++H
Sbjct: 181 QQASGAVVGSFAAVLLFLLLILFSALSLKNSH 209

BLAST of Carg10495 vs. TAIR 10
Match: AT5G06200.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 216.1 bits (549), Expect = 2.8e-56
Identity = 123/206 (59.71%), Postives = 157/206 (76.21%), Query Frame = 0

Query: 4   AESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIGALS 63
           ++S A++V +E+++       K   PLL      R   G+   KRGL+IFD +LR+ A+ 
Sbjct: 3   SDSIAVDVPAESSS-----VIKGKAPLLGL---ARDHTGSGGYKRGLSIFDFLLRLAAIV 62

Query: 64  SALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSIVCI 123
           +ALAAAATMGTS +TLPFFTQF QFEASYDDLPTFQFFV+ + IVAGY+VLSLPFS+V I
Sbjct: 63  AALAAAATMGTSDETLPFFTQFLQFEASYDDLPTFQFFVVAIAIVAGYLVLSLPFSVVTI 122

Query: 124 IRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFCQQA 183
           +RP A  PRLLLL+LD+ ALAL TAAA AAAA+V LAH+GN++TNWL +C QFGDFCQ+ 
Sbjct: 123 VRPLAVAPRLLLLVLDTAALALDTAAASAAAAIVYLAHNGNTNTNWLPICQQFGDFCQKT 182

Query: 184 SGAVVGSFAAVLLFLLLILFSALSLK 210
           SGAVV +FA+V    +L++ S +SLK
Sbjct: 183 SGAVVSAFASVTFLAILVVISGVSLK 200

BLAST of Carg10495 vs. TAIR 10
Match: AT3G11550.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 208.4 bits (529), Expect = 5.7e-54
Identity = 121/209 (57.89%), Postives = 156/209 (74.64%), Query Frame = 0

Query: 1   MKNAESSAINVASETTNSGPIGKKKSTTPLLAAEVPIRPDRGTHRMKRGLAIFDIVLRIG 60
           MKN ES+ I+V +E++++      K   PL+          G+    RGLAIFD +LR+ 
Sbjct: 1   MKN-ESTFIDVPAESSSA-----MKGKAPLIGV-ARDHTTSGSGGYNRGLAIFDFLLRLA 60

Query: 61  ALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMGIVAGYVVLSLPFSI 120
           A+ +ALAAAATMGTS +TLPFFTQF QFEASYDDLPTFQFFVI M +V GY+VLSLP S+
Sbjct: 61  AIVAALAAAATMGTSDETLPFFTQFLQFEASYDDLPTFQFFVIAMALVGGYLVLSLPISV 120

Query: 121 VCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSSTNWLAVCNQFGDFC 180
           V I+RP A  PRLLLL+LD+  LAL+TAAA +AAA+  LAHSGN +TNWL +C QFGDFC
Sbjct: 121 VTILRPLATAPRLLLLVLDTGVLALNTAAASSAAAISYLAHSGNQNTNWLPICQQFGDFC 180

Query: 181 QQASGAVVGSFAAVLLFLLLILFSALSLK 210
           Q++SGAVV +F +V+ F +L++ S ++LK
Sbjct: 181 QKSSGAVVSAFVSVVFFTILVVISGVALK 202

BLAST of Carg10495 vs. TAIR 10
Match: AT2G36100.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 189.1 bits (479), Expect = 3.6e-48
Identity = 98/163 (60.12%), Postives = 128/163 (78.53%), Query Frame = 0

Query: 47  KRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMG 106
           KRGLAIFD +LR+ A++  + AA+ M T+ +TLPFFTQF QF+A YDDLP FQ+FVI + 
Sbjct: 42  KRGLAIFDFLLRLAAIAVTIGAASVMYTAEETLPFFTQFLQFQAGYDDLPAFQYFVIAVA 101

Query: 107 IVAGYVVLSLPFSIVCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSS 166
           +VA Y+VLSLPFSIV I+RPHA  PRL+LLI D++ + L+T+AA AAA++  LAH+GN S
Sbjct: 102 VVASYLVLSLPFSIVSIVRPHAVAPRLILLICDTLVVTLNTSAAAAAASITYLAHNGNQS 161

Query: 167 TNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLK 210
           TNWL +C QFGDFCQ  S AVV    A+L F++LI+ SA++LK
Sbjct: 162 TNWLPICQQFGDFCQNVSTAVVADSIAILFFIVLIIISAIALK 204

BLAST of Carg10495 vs. TAIR 10
Match: AT2G27370.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 182.2 bits (461), Expect = 4.4e-46
Identity = 101/163 (61.96%), Postives = 123/163 (75.46%), Query Frame = 0

Query: 47  KRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVMG 106
           KRG+AIFD VLR+ A  +A+AAAA M T+ +TLPFFTQF QF+A Y DLPT   FVIV  
Sbjct: 56  KRGVAIFDFVLRLIAAITAMAAAAKMATTEETLPFFTQFLQFQADYTDLPTMSSFVIVNS 115

Query: 107 IVAGYVVLSLPFSIVCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNSS 166
           IV GY+ LSLPFSIVCI+RP A  PRL L++ D+V + L+  AA A+AA+V LAH+GNSS
Sbjct: 116 IVGGYLTLSLPFSIVCILRPLAVPPRLFLILCDTVMMGLTLMAASASAAIVYLAHNGNSS 175

Query: 167 TNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLK 210
           +NWL VC QFGDFCQ  SGAVV SF A  L + L++ SA +LK
Sbjct: 176 SNWLPVCQQFGDFCQGTSGAVVASFIAATLLMFLVILSAFALK 218

BLAST of Carg10495 vs. TAIR 10
Match: AT5G15290.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 153.3 bits (386), Expect = 2.2e-37
Identity = 80/167 (47.90%), Postives = 117/167 (70.06%), Query Frame = 0

Query: 46  MKRGLAIFDIVLRIGALSSALAAAATMGTSGQTLPFFTQFFQFEASYDDLPTFQFFVIVM 105
           M R +AI + +LRI A  + + +A  MGT+ +TLPFFTQF +F+A Y+DLP   FFV+  
Sbjct: 21  MSRRIAILEFILRIVAFFNTIGSAILMGTTHETLPFFTQFIRFQAEYNDLPALTFFVVAN 80

Query: 106 GIVAGYVVLSLPFSIVCIIRPHAAGPRLLLLILDSVALALSTAAAGAAAAVVALAHSGNS 165
            +V+GY++LSL  + V I++      R+LL+ILD   L L T+ A +AAA+V LAH+GN+
Sbjct: 81  AVVSGYLILSLTLAFVHIVKRKTQNTRILLIILDVAMLGLLTSGASSAAAIVYLAHNGNN 140

Query: 166 STNWLAVCNQFGDFCQQASGAVVGSFAAVLLFLLLILFSALSLKSAH 213
            TNW A+C QF  FC++ SG+++GSF A++L +LLIL SA++L   H
Sbjct: 141 KTNWFAICQQFNSFCERISGSLIGSFIAIVLLILLILLSAIALSRRH 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034486.16.3e-103100.00hypothetical protein SDJN02_04216, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023544952.11.4e-10299.53casparian strip membrane protein 1 [Cucurbita pepo subsp. pepo][more]
XP_022977901.12.4e-10299.06casparian strip membrane protein 1 [Cucurbita maxima][more]
KAG6604335.14.1e-10299.06hypothetical protein SDJN03_04944, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022926164.11.2e-10199.06casparian strip membrane protein 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P0DI611.5e-9186.79Casparian strip membrane protein 1 OS=Cucumis melo OX=3656 PE=2 SV=1[more]
B9IIR51.8e-6068.65Casparian strip membrane protein 2 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_... [more]
B9IIR44.4e-5963.73Casparian strip membrane protein 3 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_... [more]
B9SX129.9e-5963.73Casparian strip membrane protein 1 OS=Ricinus communis OX=3988 GN=RCOM_1259250 P... [more]
B9HBX28.3e-5860.68Casparian strip membrane protein 1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_... [more]
Match NameE-valueIdentityDescription
A0A6J1IL911.2e-10299.06CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111478054 PE=3 SV=1[more]
A0A6J1EEC65.7e-10299.06CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433348 PE=3 SV=1[more]
A0A6J1BV791.1e-8987.98CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111005840 PE=3 SV=1[more]
A0A0A0KKJ81.5e-8986.79CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G453800 PE=3 SV=1[more]
A0A5A7SKE25.6e-8986.79CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00... [more]
Match NameE-valueIdentityDescription
AT5G06200.12.8e-5659.71Uncharacterised protein family (UPF0497) [more]
AT3G11550.15.7e-5457.89Uncharacterised protein family (UPF0497) [more]
AT2G36100.13.6e-4860.12Uncharacterised protein family (UPF0497) [more]
AT2G27370.14.4e-4661.96Uncharacterised protein family (UPF0497) [more]
AT5G15290.12.2e-3747.90Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 48..196
e-value: 1.3E-44
score: 151.6
IPR006459Casparian strip membrane proteinTIGRFAMTIGR01569TIGR01569coord: 55..207
e-value: 1.9E-40
score: 136.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR11615:SF281CASPARIAN STRIP MEMBRANE PROTEIN 3coord: 19..210
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 19..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10495-RACarg10495-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007043 cell-cell junction assembly
biological_process GO:0042545 cell wall modification
cellular_component GO:0048226 Casparian strip
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding