Carg10449 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10449
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	carotene epsilon-monooxygenase, chloroplastic
Location	Carg_Chr03: 8724348 .. 8725427 (+)
RNA-Seq Expression	Carg10449
Synteny	Carg10449

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAATCCCATCTCCATATCCCTCCATTAAATCACCTTCCATTGCCGAAGGGAATCCCCCCACGCCCACGACCAGTGGTGCAAAATCCGGTTCTTGGGTCAGCCCTGATTGCCTCACCTCTCCGACTCGCTTCATTACTGATCTTCTCGGCCCTGCTCTTTTTCTTTCCCTCTTCAATTGGATGAATGACTATGGACCCATTTACAGGCTCGCCGCTGGCCCTAGAAATTTTGTCGTGGTTAGTGACCCCGCCATTGCTAACCACGTTCTCAGCAACTATGGGACTCACGCTAAAGGGCTTGTTTCTGATGTCTCCGAGTTCGTGTCTGGGTCGGGTTTCGCGATTGCAGAAGGCCCTCTTTGGACGGTAATCTTTTTGCTTTTGCTAACTGTTCTGTTGATGAGCAAAGACAAATTGAAAATTTATCATCGTGTATTGCATTCTTAGTAACTATTAATTTACGGTGATCAGGAGAAGTGTTGAAGAACTCATTGCAAATTGCAAAGAAATTGTGGAAACTGAGGAGGAACATGTGGATGACGCTGATCCAAGCTTCCTTTGATTTTTGCTGGCCAGTACACAAGAGGTGTGTTCTTGATAGCTTTAAAGTTTTCCATTCATTACCAACATTTTGCAAAATCAATCAAGAACCGAATACAGAATGAATACTTACATCCTTACTAAAATTGAAGTGAAAATCATTTATTTGTCTCAATCATTCTCAAGTATTCTTTTGTTGCCAGTTTGGAAATATATTCGAACATGTTCTTGCTTTGTGAAGTGTTTTGCACATTGTTGTAACTCCCACTTATGTTTCAAGGTTTGGGATGAAGCAGAGGAGTTTATTCCAGAATTATTTGACTTGGAAGGTCCTGTCCCTAATGAAAACGATACAGATTTCAGGTACATATTCTATTTGTGTGTGGAGACTACCCTGTTGATTGTTTTAAATAGTACAGTAGACACTTACCAGGCGCTCATAGCCATGTTTGGTGCGAGAAACTTGACCATAGGTTAGTCATACTCTTAAAGGCAAGCGCCAATGCACATGCTAACATGCTCAAATGGTGGCACGAGCAT

mRNA sequence

AGAATCCCATCTCCATATCCCTCCATTAAATCACCTTCCATTGCCGAAGGGAATCCCCCCACGCCCACGACCAGTGGTGCAAAATCCGGTTCTTGGGTCAGCCCTGATTGCCTCACCTCTCCGACTCGCTTCATTACTGATCTTCTCGGCCCTGCTCTTTTTCTTTCCCTCTTCAATTGGATGAATGACTATGGACCCATTTACAGGCTCGCCGCTGGCCCTAGAAATTTTGTCGTGGTTAGTGACCCCGCCATTGCTAACCACGTTCTCAGCAACTATGGGACTCACGCTAAAGGGCTTGTTTCTGATGTCTCCGAGTTCGTGTCTGGGTCGGGTTTCGCGATTGCAGAAGGCCCTCTTTGGACGGTAATCTTTTTGCTTTTGCTAACTGTTCTGTTGATGAGCAAAGACAAATTGAAAATTTATCATCGAGAAGTGTTGAAGAACTCATTGCAAATTGCAAAGAAATTGTGGAAACTGAGGAGGAACATGTGGATGACGCTGATCCAAGCTTCCTTTGATTTTTGCTGGCCAGTACACAAGAGTTTTCCATTCATTACCAACATTTTGCAAAATCAATCAAGAACCGAATACAGAATGAATACTTACATCCTTACTAAAATTGAAGTGAAAATCATTTATTTGTCTCAATCATTCTCAAGTATTCTTTTGTTGCCAGTTTGGAAATATATTCGAACATGTTCTTGCTTTGTGAAGTGTTTTGCACATTGTTGTAACTCCCACTTATGTTTCAAGGTTTGGGATGAAGCAGAGGAGTTTATTCCAGAATTATTTGACTTGGAAGGTCCTGTCCCTAATGAAAACGATACAGATTTCAGACACTTACCAGGCGCTCATAGCCATGTTTGGTGCGAGAAACTTGACCATAGGTTAGTCATACTCTTAAAGGCAAGCGCCAATGCACATGCTAACATGCTCAAATGGTGGCACGAGCAT

Coding sequence (CDS)

AGAATCCCATCTCCATATCCCTCCATTAAATCACCTTCCATTGCCGAAGGGAATCCCCCCACGCCCACGACCAGTGGTGCAAAATCCGGTTCTTGGGTCAGCCCTGATTGCCTCACCTCTCCGACTCGCTTCATTACTGATCTTCTCGGCCCTGCTCTTTTTCTTTCCCTCTTCAATTGGATGAATGACTATGGACCCATTTACAGGCTCGCCGCTGGCCCTAGAAATTTTGTCGTGGTTAGTGACCCCGCCATTGCTAACCACGTTCTCAGCAACTATGGGACTCACGCTAAAGGGCTTGTTTCTGATGTCTCCGAGTTCGTGTCTGGGTCGGGTTTCGCGATTGCAGAAGGCCCTCTTTGGACGGTAATCTTTTTGCTTTTGCTAACTGTTCTGTTGATGAGCAAAGACAAATTGAAAATTTATCATCGAGAAGTGTTGAAGAACTCATTGCAAATTGCAAAGAAATTGTGGAAACTGAGGAGGAACATGTGGATGACGCTGATCCAAGCTTCCTTTGATTTTTGCTGGCCAGTACACAAGAGTTTTCCATTCATTACCAACATTTTGCAAAATCAATCAAGAACCGAATACAGAATGAATACTTACATCCTTACTAAAATTGAAGTGAAAATCATTTATTTGTCTCAATCATTCTCAAGTATTCTTTTGTTGCCAGTTTGGAAATATATTCGAACATGTTCTTGCTTTGTGAAGTGTTTTGCACATTGTTGTAACTCCCACTTATGTTTCAAGGTTTGGGATGAAGCAGAGGAGTTTATTCCAGAATTATTTGACTTGGAAGGTCCTGTCCCTAATGAAAACGATACAGATTTCAGACACTTACCAGGCGCTCATAGCCATGTTTGGTGCGAGAAACTTGACCATAGGTTAGTCATACTCTTAAAGGCAAGCGCCAATGCACATGCTAACATGCTCAAATGGTGGCACGAGCAT

Protein sequence

RIPSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRFITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSGFAIAEGPLWTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAKKLWKLRRNMWMTLIQASFDFCWPVHKSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYLSQSFSSILLLPVWKYIRTCSCFVKCFAHCCNSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLPGAHSHVWCEKLDHRLVILLKASANAHANMLKWWHEH

Homology

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BLAST of Carg10449 vs. NCBI nr
Match: KAG7034532.1 (Carotene epsilon-monooxygenase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661.0 bits (1704), Expect = 5.2e-186
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRFITDLLGPALFLSLFNW 60
           RIPSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRFITDLLGPALFLSLFNW
Sbjct: 1   RIPSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRFITDLLGPALFLSLFNW 60

Query: 61  MNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSGFAIAEGPL 120
           MNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSGFAIAEGPL
Sbjct: 61  MNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSGFAIAEGPL 120

Query: 121 WTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAKKLWKLRRNMWMTLIQASFDFCWPVH 180
           WTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAKKLWKLRRNMWMTLIQASFDFCWPVH
Sbjct: 121 WTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAKKLWKLRRNMWMTLIQASFDFCWPVH 180

Query: 181 KSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYLSQSFSSILLLPVWKYIRTCSCFVKC 240
           KSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYLSQSFSSILLLPVWKYIRTCSCFVKC
Sbjct: 181 KSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYLSQSFSSILLLPVWKYIRTCSCFVKC 240

Query: 241 FAHCCNSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLPGAHSHVWCEKLDHRLVI 300
           FAHCCNSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLPGAHSHVWCEKLDHRLVI
Sbjct: 241 FAHCCNSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLPGAHSHVWCEKLDHRLVI 300

Query: 301 LLKASANAHANMLKWWHEH 320
           LLKASANAHANMLKWWHEH
Sbjct: 301 LLKASANAHANMLKWWHEH 319

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BLAST of Carg10449 vs. NCBI nr
Match: XP_022143881.1 (carotene epsilon-monooxygenase, chloroplastic [Momordica charantia])

HSP 1 Score: 182.6 bits (462), Expect = 5.4e-42
Identity = 142/350 (40.57%), Postives = 179/350 (51.14%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTPT-----TSGAKSGSWVSPDCLTSPTRFIT--------- 60
           RIP P+ SIKS SI EGNPPTPT     T+ AKSGSWVSPD LTS TR+IT         
Sbjct: 26  RIPPPFLSIKS-SIDEGNPPTPTKLKNSTNNAKSGSWVSPDWLTSLTRYITLGQGDDSGI 85

Query: 61  -----------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGT 120
                      DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT
Sbjct: 86  PIASAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGT 145

Query: 121 HAKGLVSDVSEFVSGSGFAIAEGPLWTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAK 180
           +AKGLVS+VSEF+ GSGFAIAEGPLWTV    ++   L  K    I  R   K ++++ +
Sbjct: 146 YAKGLVSEVSEFLFGSGFAIAEGPLWTVRRRAVVP-SLHKKYLSVIVDRVFCKCAMRLVE 205

Query: 181 KLWKLRRNMWMTLIQASFDFCWPVHKSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYL 240
           KL K           A  +    + + F  +T  +   S   Y  ++       +  +Y 
Sbjct: 206 KLQK----------DALNNNSVNMEEKFSQLTLDIIGLSVFNYSFDSLSADSPVIDAVYT 265

Query: 241 S---QSFSSILLLPVWKYIRTCSCFVKCFAHCCNSHLCFKVWDEAEEFIPELFDLEGPVP 300
           +       S  +LP WK    C    +         +  +  +E      E+ + EG   
Sbjct: 266 ALKEAEARSTDILPYWKIKALCKIIPRQIKAEEAVTVIRRTVEELIAKCKEIVETEGERI 325

Query: 301 NE----NDTD---FRHLPGAHSHVWCEKLDHRLVILLKASANAHANMLKW 316
           +E    NDTD    R L  +   V   +L   L+ +L A      ++L W
Sbjct: 326 DEEEYVNDTDPSILRFLLASRQEVSSVQLRDDLLSMLVAGHETTGSVLTW 363

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BLAST of Carg10449 vs. NCBI nr
Match: XP_022143881.1 (carotene epsilon-monooxygenase, chloroplastic [Momordica charantia])

HSP 1 Score: 63.2 bits (152), Expect = 4.8e-06
Identity = 26/38 (68.42%), Postives = 32/38 (84.21%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEFIPE FDLEGPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWEQAEEFIPERFDLEGPVPNESNTDFRFIP 484

HSP 2 Score: 176.0 bits (445), Expect = 5.1e-40
Identity = 99/146 (67.81%), Postives = 106/146 (72.60%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTP---TTSGAKSGSWVSPDCLTSPTRFIT----------- 60
           R PSP+P IKS SI EG  P+    +T  AKSGSWVSPD LTS TR+IT           
Sbjct: 26  RTPSPFPFIKS-SIDEGQNPSKLKNSTKTAKSGSWVSPDWLTSLTRYITLGQGDDSGIPI 85

Query: 61  ---------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHA 120
                    DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT+A
Sbjct: 86  ATAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGTYA 145

Query: 121 KGLVSDVSEFVSGSGFAIAEGPLWTV 124
           KGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 146 KGLVSEVSEFLFGSGFAIAEGPLWTV 170

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BLAST of Carg10449 vs. NCBI nr
Match: XP_038882587.1 (carotene epsilon-monooxygenase, chloroplastic [Benincasa hispida])

HSP 1 Score: 60.5 bits (145), Expect = 3.1e-05
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEFIPE FDL GPVPNE++TDFR +P
Sbjct: 445 NIHRSSQVWEQAEEFIPERFDLGGPVPNESNTDFRFIP 482

HSP 2 Score: 172.9 bits (437), Expect = 4.3e-39
Identity = 98/148 (66.22%), Postives = 107/148 (72.30%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT--------- 60
           R   P+ SIKS SI E +PPTP     +T+ +KSGSWVSPD LTS TR+IT         
Sbjct: 26  RTQFPFLSIKS-SIDERDPPTPAKLNNSTNTSKSGSWVSPDWLTSLTRYITLGQGDDSGI 85

Query: 61  -----------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGT 120
                      DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT
Sbjct: 86  PIASAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGT 145

Query: 121 HAKGLVSDVSEFVSGSGFAIAEGPLWTV 124
           +AKGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 146 YAKGLVSEVSEFLFGSGFAIAEGPLWTV 172

[Close]

BLAST of Carg10449 vs. NCBI nr
Match: XP_022972774.1 (carotene epsilon-monooxygenase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 61.2 bits (147), Expect = 1.8e-05
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW+ AEEFIPE FDL+GPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWERAEEFIPERFDLDGPVPNESNTDFRFIP 484

HSP 2 Score: 172.2 bits (435), Expect = 7.4e-39
Identity = 96/146 (65.75%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT----------- 62
           P PYPSIKS     GNP TP      T+  KS SWVSPD LTS TR IT           
Sbjct: 28  PFPYPSIKSSLDERGNPSTPPKLKNPTNAPKSRSWVSPDWLTSLTRSITLGQGDDSGIPI 87

Query: 63  ---------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHA 122
                    DLLG ALFL LF WMNDYGPIYRLAAGPRNFV+VSDPAIA HVL NYGT+A
Sbjct: 88  ATAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYGTYA 147

Query: 123 KGLVSDVSEFVSGSGFAIAEGPLWTV 124
           KGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 148 KGLVSEVSEFLFGSGFAIAEGPLWTV 173

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BLAST of Carg10449 vs. ExPASy Swiss-Prot
Match: Q6TBX7 (Carotene epsilon-monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97C1 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.8e-33
Identity = 79/140 (56.43%), Postives = 91/140 (65.00%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRF------------------ 62
           P P  +    S  E   P   T+ +KS SWVSPD LT+ TR                   
Sbjct: 27  PKPKFTFSIRSSIEKPKPKLETNSSKSQSWVSPDWLTTLTRTLSSGKNDESGIPIANAKL 86

Query: 63  --ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVS 122
             + DLLG ALFL L+ WMN+YGPIYRLAAGPRNFV+VSDPAIA HVL NY  +AKGLV+
Sbjct: 87  DDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYPKYAKGLVA 146

HSP 2 Score: 55.1 bits (131), Expect = 1.7e-06
Identity = 20/38 (52.63%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEF+PE FD++G +PNE +TDF+ +P
Sbjct: 442 NIHRSSEVWEKAEEFLPERFDIDGAIPNETNTDFKFIP 479

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BLAST of Carg10449 vs. ExPASy Swiss-Prot
Match: O48921 (Cytochrome P450 97B2, chloroplastic OS=Glycine max OX=3847 GN=CYP97B2 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.6e-12
Identity = 34/78 (43.59%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           ++DLLG  LF SL++W  ++G +Y+LA GP+ FVVVSDP +A H+L  N  ++ KG+++D
Sbjct: 86  VSDLLGRPLFFSLYDWFLEHGAVYKLAFGPKAFVVVSDPIVARHILRENAFSYDKGVLAD 145

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 146 ILEPIMGKGLIPADLDTW 163

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BLAST of Carg10449 vs. ExPASy Swiss-Prot
Match: O23365 (Cytochrome P450 97B3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97B3 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 2.7e-12
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           ++DL G  LFLSL++W  ++G IY+LA GP+ FVV+SDP IA HVL  N  ++ KG++++
Sbjct: 91  VSDLFGKPLFLSLYDWFLEHGGIYKLAFGPKAFVVISDPIIARHVLRENAFSYDKGVLAE 150

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 151 ILEPIMGKGLIPADLDTW 168

[Close]

BLAST of Carg10449 vs. ExPASy Swiss-Prot
Match: Q43078 (Cytochrome P450 97B1, chloroplastic OS=Pisum sativum OX=3888 GN=CYP97B1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           +TDL    LF SL++W  ++G +Y+LA GP+ FVVVSDP +A H+L  N  ++ KG+++D
Sbjct: 95  VTDLFDRPLFFSLYDWFLEHGSVYKLAFGPKAFVVVSDPIVARHILRENAFSYDKGVLAD 154

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 155 ILEPIMGKGLIPADLETW 172

[Close]

BLAST of Carg10449 vs. ExPASy Swiss-Prot
Match: Q93VK5 (Protein LUTEIN DEFICIENT 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97A3 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.6e-10
Identity = 29/71 (40.85%), Postives = 48/71 (67.61%), Query Frame = 0


Query: 52  ALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSDVSEFVSG 111
           A F+ L+     YG I+RL  GP++F++VSDP+IA H+L  N   ++KG+++++ +FV G
Sbjct: 127 AFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNAKAYSKGILAEILDFVMG 186

Query: 112 SGFAIAEGPLW 122
            G   A+G +W
Sbjct: 187 KGLIPADGEIW 197

[Close]

BLAST of Carg10449 vs. ExPASy TrEMBL
Match: A0A6J1CRM7 (carotene epsilon-monooxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013687 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 2.6e-42
Identity = 142/350 (40.57%), Postives = 179/350 (51.14%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTPT-----TSGAKSGSWVSPDCLTSPTRFIT--------- 60
           RIP P+ SIKS SI EGNPPTPT     T+ AKSGSWVSPD LTS TR+IT         
Sbjct: 26  RIPPPFLSIKS-SIDEGNPPTPTKLKNSTNNAKSGSWVSPDWLTSLTRYITLGQGDDSGI 85

Query: 61  -----------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGT 120
                      DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT
Sbjct: 86  PIASAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGT 145

Query: 121 HAKGLVSDVSEFVSGSGFAIAEGPLWTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAK 180
           +AKGLVS+VSEF+ GSGFAIAEGPLWTV    ++   L  K    I  R   K ++++ +
Sbjct: 146 YAKGLVSEVSEFLFGSGFAIAEGPLWTVRRRAVVP-SLHKKYLSVIVDRVFCKCAMRLVE 205

Query: 181 KLWKLRRNMWMTLIQASFDFCWPVHKSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYL 240
           KL K           A  +    + + F  +T  +   S   Y  ++       +  +Y 
Sbjct: 206 KLQK----------DALNNNSVNMEEKFSQLTLDIIGLSVFNYSFDSLSADSPVIDAVYT 265

Query: 241 S---QSFSSILLLPVWKYIRTCSCFVKCFAHCCNSHLCFKVWDEAEEFIPELFDLEGPVP 300
           +       S  +LP WK    C    +         +  +  +E      E+ + EG   
Sbjct: 266 ALKEAEARSTDILPYWKIKALCKIIPRQIKAEEAVTVIRRTVEELIAKCKEIVETEGERI 325

Query: 301 NE----NDTD---FRHLPGAHSHVWCEKLDHRLVILLKASANAHANMLKW 316
           +E    NDTD    R L  +   V   +L   L+ +L A      ++L W
Sbjct: 326 DEEEYVNDTDPSILRFLLASRQEVSSVQLRDDLLSMLVAGHETTGSVLTW 363

[Close]

BLAST of Carg10449 vs. ExPASy TrEMBL
Match: A0A6J1CRM7 (carotene epsilon-monooxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013687 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-06
Identity = 26/38 (68.42%), Postives = 32/38 (84.21%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEFIPE FDLEGPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWEQAEEFIPERFDLEGPVPNESNTDFRFIP 484

HSP 2 Score: 172.9 bits (437), Expect = 2.1e-39
Identity = 98/148 (66.22%), Postives = 107/148 (72.30%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT--------- 60
           R   P+ SIKS SI E +PPTP     +T+ +KSGSWVSPD LTS TR+IT         
Sbjct: 26  RTQFPFLSIKS-SIDERDPPTPAKLNNSTNTSKSGSWVSPDWLTSLTRYITLGQGDDSGI 85

Query: 61  -----------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGT 120
                      DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT
Sbjct: 86  PIASAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGT 145

Query: 121 HAKGLVSDVSEFVSGSGFAIAEGPLWTV 124
           +AKGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 146 YAKGLVSEVSEFLFGSGFAIAEGPLWTV 172

[Close]

BLAST of Carg10449 vs. ExPASy TrEMBL
Match: A0A6J1ICJ1 (carotene epsilon-monooxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111471281 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 8.9e-06
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW+ AEEFIPE FDL+GPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWERAEEFIPERFDLDGPVPNESNTDFRFIP 484

HSP 2 Score: 172.2 bits (435), Expect = 3.6e-39
Identity = 96/146 (65.75%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT----------- 62
           P PYPSIKS     GNP TP      T+  KS SWVSPD LTS TR IT           
Sbjct: 28  PFPYPSIKSSLDERGNPSTPPKLKNPTNAPKSRSWVSPDWLTSLTRSITLGQGDDSGIPI 87

Query: 63  ---------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHA 122
                    DLLG ALFL LF WMNDYGPIYRLAAGPRNFV+VSDPAIA HVL NYGT+A
Sbjct: 88  ATAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYGTYA 147

Query: 123 KGLVSDVSEFVSGSGFAIAEGPLWTV 124
           KGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 148 KGLVSEVSEFLFGSGFAIAEGPLWTV 173

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BLAST of Carg10449 vs. ExPASy TrEMBL
Match: A0A5A7SKS2 (Carotene epsilon-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001720 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 3.6e-39
Identity = 96/146 (65.75%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT----------- 62
           P PYPSIKS     GNP TP      T+  KS SWVSPD LTS TR IT           
Sbjct: 28  PFPYPSIKSSLDERGNPSTPPKLKNPTNAPKSRSWVSPDWLTSLTRSITLGQGDDSGIPI 87

Query: 63  ---------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHA 122
                    DLLG ALFL LF WMNDYGPIYRLAAGPRNFV+VSDPAIA HVL NYGT+A
Sbjct: 88  ATAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYGTYA 147

Query: 123 KGLVSDVSEFVSGSGFAIAEGPLWTV 124
           KGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 148 KGLVSEVSEFLFGSGFAIAEGPLWTV 173

[Close]

BLAST of Carg10449 vs. ExPASy TrEMBL
Match: A0A1S3CIN0 (carotene epsilon-monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500849 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 3.6e-39
Identity = 96/146 (65.75%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT----------- 62
           P PYPSIKS     GNP TP      T+  KS SWVSPD LTS TR IT           
Sbjct: 28  PFPYPSIKSSLDERGNPSTPPKLKNPTNAPKSRSWVSPDWLTSLTRSITLGQGDDSGIPI 87

Query: 63  ---------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHA 122
                    DLLG ALFL LF WMNDYGPIYRLAAGPRNFV+VSDPAIA HVL NYGT+A
Sbjct: 88  ATAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYGTYA 147

Query: 123 KGLVSDVSEFVSGSGFAIAEGPLWTV 124
           KGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 148 KGLVSEVSEFLFGSGFAIAEGPLWTV 173

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BLAST of Carg10449 vs. TAIR 10
Match: AT3G53130.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 143.3 bits (360), Expect = 3.4e-34
Identity = 79/140 (56.43%), Postives = 91/140 (65.00%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRF------------------ 62
           P P  +    S  E   P   T+ +KS SWVSPD LT+ TR                   
Sbjct: 27  PKPKFTFSIRSSIEKPKPKLETNSSKSQSWVSPDWLTTLTRTLSSGKNDESGIPIANAKL 86

Query: 63  --ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVS 122
             + DLLG ALFL L+ WMN+YGPIYRLAAGPRNFV+VSDPAIA HVL NY  +AKGLV+
Sbjct: 87  DDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYPKYAKGLVA 146

HSP 2 Score: 55.1 bits (131), Expect = 1.2e-07
Identity = 20/38 (52.63%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEF+PE FD++G +PNE +TDF+ +P
Sbjct: 442 NIHRSSEVWEKAEEFLPERFDIDGAIPNETNTDFKFIP 479

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BLAST of Carg10449 vs. TAIR 10
Match: AT4G15110.1 (cytochrome P450, family 97, subfamily B, polypeptide 3 )

HSP 1 Score: 74.3 bits (181), Expect = 1.9e-13
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           ++DL G  LFLSL++W  ++G IY+LA GP+ FVV+SDP IA HVL  N  ++ KG++++
Sbjct: 91  VSDLFGKPLFLSLYDWFLEHGGIYKLAFGPKAFVVISDPIIARHVLRENAFSYDKGVLAE 150

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 151 ILEPIMGKGLIPADLDTW 168

[Close]

BLAST of Carg10449 vs. TAIR 10
Match: AT1G31800.1 (cytochrome P450, family 97, subfamily A, polypeptide 3 )

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-11
Identity = 29/71 (40.85%), Postives = 48/71 (67.61%), Query Frame = 0


Query: 52  ALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSDVSEFVSG 111
           A F+ L+     YG I+RL  GP++F++VSDP+IA H+L  N   ++KG+++++ +FV G
Sbjct: 127 AFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNAKAYSKGILAEILDFVMG 186

Query: 112 SGFAIAEGPLW 122
            G   A+G +W
Sbjct: 187 KGLIPADGEIW 197

HSP 2 Score: 42.0 bits (97), Expect = 1.1e-03
Identity = 19/43 (44.19%), Postives = 26/43 (60.47%), Query Frame = 0


Query: 241 FAHCCNSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           F    N H     WD+AE+F PE + L+GP PNE + +F +LP
Sbjct: 466 FISVWNLHRSPLHWDDAEKFNPERWPLDGPNPNETNQNFSYLP 508

The following BLAST results are available for this feature:

BLAST of Carg10449 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034532.1	5.2e-186	100.00	Carotene epsilon-monooxygenase, chloroplastic, partial [Cucurbita argyrosperma s...	[more]
XP_022143881.1	5.4e-42	40.57	carotene epsilon-monooxygenase, chloroplastic [Momordica charantia]	[more]
XP_022143881.1	4.8e-06	68.42	carotene epsilon-monooxygenase, chloroplastic [Momordica charantia]	[more]
XP_038882587.1	3.1e-05	65.79	carotene epsilon-monooxygenase, chloroplastic [Benincasa hispida]	[more]
XP_022972774.1	1.8e-05	65.79	carotene epsilon-monooxygenase, chloroplastic [Cucurbita maxima]	[more]

BLAST of Carg10449 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6TBX7	4.8e-33	56.43	Carotene epsilon-monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O48921	1.6e-12	43.59	Cytochrome P450 97B2, chloroplastic OS=Glycine max OX=3847 GN=CYP97B2 PE=2 SV=1	[more]
O23365	2.7e-12	44.87	Cytochrome P450 97B3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97B3 P...	[more]
Q43078	1.0e-11	42.31	Cytochrome P450 97B1, chloroplastic OS=Pisum sativum OX=3888 GN=CYP97B1 PE=2 SV=...	[more]
Q93VK5	2.6e-10	40.85	Protein LUTEIN DEFICIENT 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP...	[more]

BLAST of Carg10449 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CRM7	2.6e-42	40.57	carotene epsilon-monooxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1CRM7	2.3e-06	68.42	carotene epsilon-monooxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1ICJ1	8.9e-06	65.79	carotene epsilon-monooxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A5A7SKS2	3.6e-39	65.75	Carotene epsilon-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A1S3CIN0	3.6e-39	65.75	carotene epsilon-monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1035...	[more]

BLAST of Carg10449 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53130.1	3.4e-34	56.43	Cytochrome P450 superfamily protein	[more]
AT4G15110.1	1.9e-13	44.87	cytochrome P450, family 97, subfamily B, polypeptide 3	[more]
AT1G31800.1	1.8e-11	40.85	cytochrome P450, family 97, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 56..143 e-value: 1.5E-6 score: 27.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 39..233 e-value: 2.5E-9 score: 38.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 49..234
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	PANTHER	PTHR24291	CYTOCHROME P450 FAMILY 4	coord: 50..123
None	No IPR available	PANTHER	PTHR24291:SF137	SUBFAMILY NOT NAMED	coord: 50..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10449-RA	Carg10449-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen