Carg10251 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10251
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCCA-adding enzyme
LocationCarg_Chr10: 97353 .. 102365 (-)
RNA-Seq ExpressionCarg10251
SyntenyCarg10251
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTATCTCCCTCGACGGACACCACTCTTCCGTTTCCTCCGCAAGGTAATTCTGCTCCTATTGCCATTTCCTTTGCTTTCTCTTCCCTTCCCTCTTTCACCTGATTCATTCACCTTATTTACCTCAGCTTCGTCCCACTTCCATTGCAGTGGGGAAGACAGCAGCTCCTCAAACAGATACCTTCTTCAACGGCCACACCAATTCTCCGCCTCCCCCAAGGCAATTTCAAGGGTTTTTCCTCCTACTTTCTAACTACTTTTAAGTTTCATAACTAGCTTCTTCTTGTTTCTGATAATATGAAACTAGGCCTCTGTTTCATTCTTAATTTTTTTTGACAAAAATAAAAAAAAATAATAAATAAATAAAAACTGTTATTTTGCTGAAGAATTCGATGATAATGATTCTAAGCTGCGGAAGTGGAAGACGTTTTCTTCCAAGGAGCTTGGGATTGATATTTTCATGATTCCCAAACCTACCAGAAAAGTTCTGAACGGGCTCAAGAAAAAAGGTTACCCTACGTTGTCTCCTTCATTTACAAACGTCTTCTCTCACATCAAATAGTAAATAAACATTTAGTGTCATCTTCAAAATCTTAGCCTTTATCACCTTCTTCATGCACTTCTAGTATTTTGTAACTTTTGTAATTAATCCTGTGATTATTGTTTCACGTTCTAGGTTACGAAGTTTACCTTGTAGGAGGTTGTGTTCGCGATCTTATCTTGAACAGAACACCAAAGGATTTTGACATAATAACTTCAGCTGAGCTTAAGGAGGTTTGTCTCCAACCATCTCCTTCAAAATATTTTGTATTACTTATATCGCTACCGAGGAAACTTTATTATCTATCATAAATACATCGAGTTGTGAGAAGAGACACATACACATGATAAATTAGTTTTCTCATATATATATATATATATTTTTTTTGATGATTAAAGAAGAATAGAAAGGAAACCCGTTAGATTAAAAAAGAATACTATTGGAAATCAGATTGCGAGTTGAAATTTCTACCATCTATCCTTACGAGACACTTCTCATTTCAATTCTTTATGCCGGAATGGTCAAGTGTGTTTTGTAGAATATGACGAGGCAGATTACTGGAACTTGGATACTGAATATGTCCTTTATGTTTGGCCAAGATTATAATGTAAATTACATAAATCTCCGTGGCAAAAGGCCTCAATATGTTAGAAACTTTTTTGGGGGTCAAAAGCTCGATGAATATACATTGCAAGTTTCAGGTTCAACAGGGACTACTGACACTGTGAAGCCTCAGTTATGCCTGTTTTCTCAATTTTAGTTATAAGTACTTTATATATATAATTTAAGGTTCGGCCCCTCATTTTTAACATATATTATATCGTACCATTTAAAGGTTTCAAGAACATTTTCATGGTGTGAAATAGTTGGGAGGAAGTTTCCTATATGCCATGTGCATATTGATGGTAGCATTGTAGAGGTTCGTTTCTCTTCCTATTAATAAATGATTGATACCTTTGACCATATGTAGTTGTTTCTGGTAGATATTACATCAATTTGATATTTTATGATTTCGTGTTTTAATAACGAGCTTTTACTTTCTTTAAAATATTAGGTGTCAAGTTTTAGCACATCCAGTAGGCCATTTGACAGACACTTGAACTCTGCTATTGAAAAGCCAATGAACTGTGAAGAGGAAGATTATGTCCGATGGAAGAATTGCATGCAACGTGACTTTACCATTAACGGGTGGTTTACGAGATCCTGCATCTTGAGAATCCATGTTCCTATCTCTATTATACAATTGCAATATTTTTTAAATAGTAGGTTTTCTGGTAAATGAAAATTTATAATTCGTGGGTCCCCCTCCAAACACATGCAAAAGAGAAAAAAAACGGTCTCTTTCACCCTTCGGTTCTCAACTAATAGTCAGCTTCTTGCAGGCTGATGTATGATCCATACAACAGTGCAGTATACGACTACTTGGAAGGAATGGAGGATATAAAACAAGCTAAAGTATGCACACATTTCTCTCAAGTTATTACTTAAATAAATACTGAAAGCCACACTAGATGATATTGTAAACTTCTTTTGCACGACCTTCTGGGAGCATAGATACGAACAGTGGTTCCTGCCTGCACTTCCTTTCAAGAGGATTGTGGTGAGTTTTCATTTGCAGAACTCCCTTATCGTATGTTGACTAGAGCATTGAGCTGAATGTATTGTTGAGAAACATAAGTGAGCTTAAGCAGCACATATTTATGAACAAGAATTCTAATTGATTTTCTTTCCTTAAAATATCAGGCAAGGCTTCAGATGGAAATGAACTATTTGTTGTCTTATGGTTCTGCTGAAGCTTCTTTGAGGTTGTTATGGAAATATGGACTTCTAGAAATACTTCTTCCAATTCAGGTGAATTGACGCTTGACTTTTTCCCCAGTTATGGGTTACAAATAAGTGAGACAGTTTGATATTGTAGGCAGCATATTTCATCCAATATGGTTTTCGGAGGTCCGATAAGGGATCAAATATGCTTTTGGTAATTGTCTCTTTCCTATTCTTACTATTACTGTCTTTTGGGTGCTTATCAGAATTTATTTTCTTCCAAGTTTTTCCTAATGTACGTGGGAGACCGAACTTATTTTTCACTTGGTACTGCATAAGACTTGGTAGTTTGCTAATCTCACGAAAAGTTGCATGTAATAATATATATATATATATAATATATATATATATATTGTATATTATATATTATATAGTATAGTAGAAGATCAATATGATGTATGCGGTGTGGGTGGTTTTCCACAACTTTGTTATCCGAAATGTGGAAGGAAAAATTATTCATGGTTTGTTCTTCTAATGGTTAGTTGTACGTTGTGTGCTTTGAAAAAAAAATGTTTCTGGTATATTTAAAGCTTTTGAATTACTTAGTTTCCTTTACTTTCTTTCGTTCGCCCTCCCCTGTCCCCACTTCCCCAAGTCCTAAATAGCTCAAGAGTAAGTTATCTTGAATGCTTTCGGTATATTTTTCCTTGTCCTTTGAAATTATTCTGGAGTAGACAAAGTATAAATCCCTTGGTGATGTATGCTGAATCATGCATGGAGTAAAGCTTTTGAAAAGTAAGGATAGGACATCGATTATGTCAAATTGATGATTTTCCTACCTGATTATGAATTGATGACACAATTGGTCCATGGCAATTACTTGTTATTAACTAACATTGTTAGTATGCAGTCCCTATTTTCCAGCATGGATAAACTTCTGGCACCTAATAGACCTTGTCACAGTAGTTTATGGTAAGATTGTTGTTGAGAACTAAAATCTACTGTTGTACTGTTGCTATCTATAATTATCGTCAGGGTTCAACATGGTGTCAGGATGTAGTGTAAACTACTCGTGTCTGATCTACAAAATTGGAATGTACCAATGCCAGTGAAGAGTGCTTAAAAAATAGGTTTAATATGTTATTGAAATGTAAAATTATATCTCTAGGTTACCCTAGAAGAGAGCTGAAATATTTTTTCCCCTTGAGTTATTGGTTGAATTTCAAAGTTGCCGTAAAGATGGTGGTGGAGTTTTGGAGGTGATTAGATTTAATACCTCGGTTTGGGCCATGCTGTCTAAGGCCTTCTGTAATGATTAGATTGGCCTTGTTCTCTTGGACGAGAGTCAGTTTTTTTCTTTTCTTTTTTTTTTTTCTTTTTTTTTTTTTTTTCCATTTTGGTATGTGTTTTCGTATTTGATAATATTTCTCAATGAAACCACGGTTTTTTATTTTTCTTAGAAAAAGATGACAGTTGTTTAGTTTATGGCTTAAAGCTGAAACCTGCTGTTGGTCTGATCCTGTAAATTGTTCGGTGAACCATGATTCAAACTTGTTAATTAATATTTCGTTTATGAGCAGTGATAGTACTTGTTTTCCGAACTTTCTCTCTTTTGCAGTGAAGCAGTCTCAAACACTGTTTTCTCCTACAGGGTTGCAGTATTAGCCTTCCATGTTGCCCTGTCTGACCAGCCTAGGAGTCCTTTAGTGGTTGCAGCATTTAGTCTTGCAGTCCACAATGGTGGCAATATGATGGAAGCAATTAGCATTGCTAAGAGCATCAGTAGAGCACATAATGTAAACTTTCACGAATTGTTAGAACCTGAGAATCTGGAGGTCCAAACTTTGATTGATGAGGTTATGGATCTTACTACGTCTATTAAGAACGCACTTCATAAGATGACCGACGAGCACTCTGTGTCTCTAGTCTTGGAAATGTATCCCCAAGCACCAGCCTCGGATCTAGTAATTTTTCTTCATTTTAATCTCTCTTGTACTTTTCAAAATAAAAAAGGTTGTTGCGAATTGTCTATACATTCAAAGTCGATTATAATTTTAGGTTATAGAGAATATCAAGCTTATAGTAATCTACCCTACTCTTTTCTCCTGGAATATAAATTATAGGATCCAAGTTTTTTTTTTCCTACGACATATATTGCCTGTGGAATCATATGCTAAAAAGTTCACATATTGCAGGTTTTTATCCCATTGGTGGTGTACTTGAAGGTACGCAAAATTTTTGAGTGCGTTGTTGAGGGTGCAGAGAGAGTATTTGTTCCGAAGAGAGGAAAGGTTAATTACGAGTGTCTGGCTCTCGGGGACCTACCAGAACTTCGGCATTTTTTTGCAAGGATTGTACTTGATACTGTCTACCCTCTTGATCTCAATCATACTCGAGAATAAATTTTCGAGATAATTAGTAAATGGATGATTTTTGGGTCCTTTACTACAGGTATCTGACTTAAGCTTTGAGTATGTTTTGTACGCGGCCTGAGGTCAGGTTCATTGGATCATCTATTGAATCTCATAGAAGTTCAGATTTTTGTAATCTTTAATCCGGTATCTAGTAATACAGAAAGAAAAGGGAATGATGCAATCGGCAGTCGAATCCTACAAAAAGTGCTTTAGCGTCAAAAGCCTCAATTTTATGGTCCTAAGTATAATTAGAATGATTTCCAAGTCTTTGGAGTTACAGGCATCCGCATTTTT

mRNA sequence

ACCTTATCTCCCTCGACGGACACCACTCTTCCGTTTCCTCCGCAAGGTTACGAAGTTTACCTTGTAGGAGGTTGTGTTCGCGATCTTATCTTGAACAGAACACCAAAGGATTTTGACATAATAACTTCAGCTGAGCTTAAGGAGGTTTCAAGAACATTTTCATGGTGTGAAATAGTTGGGAGGAAGTTTCCTATATGCCATGTGCATATTGATGGTAGCATTGTAGAGGTGTCAAGTTTTAGCACATCCAGTAGGCCATTTGACAGACACTTGAACTCTGCTATTGAAAAGCCAATGAACTGTGAAGAGGAAGATTATGTCCGATGGAAGAATTGCATGCAACGTGACTTTACCATTAACGGGCTGATGTATGATCCATACAACAGTGCAGTATACGACTACTTGGAAGGAATGGAGGATATAAAACAAGCTAAACATAGATACGAACAGTGGTTCCTGCCTGCACTTCCTTTCAAGAGGATTGTGGTGAGTTTTCATTTGCAGAACTCCCTTATCGCAAGGCTTCAGATGGAAATGAACTATTTGTTGTCTTATGGTTCTGCTGAAGCTTCTTTGAGGTTGTTATGGAAATATGGACTTCTAGAAATACTTCTTCCAATTCAGGCAGCATATTTCATCCAATATGGTTTTCGGAGGTCCGATAAGGGATCAAATATGCTTTTGTCCCTATTTTCCAGCATGGATAAACTTCTGGCACCTAATAGACCTTGTCACAGTAGTTTATGTGAAGCAGTCTCAAACACTGTTTTCTCCTACAGGGTTGCAGTATTAGCCTTCCATGTTGCCCTGTCTGACCAGCCTAGGAGTCCTTTAGTGGTTGCAGCATTTAGTCTTGCAGTCCACAATGGTGGCAATATGATGGAAGCAATTAGCATTGCTAAGAGCATCAGTAGAGCACATAATGTAAACTTTCACGAATTGTTAGAACCTGAGAATCTGGAGGTCCAAACTTTGATTGATGAGGTTATGGATCTTACTACGTCTATTAAGAACGCACTTCATAAGATGACCGACGAGCACTCTGTGTCTCTAGTCTTGGAAATGTATCCCCAAGCACCAGCCTCGGATCTAGTTTTTATCCCATTGGTGGTGTACTTGAAGGTACGCAAAATTTTTGAGTGCGTTGTTGAGGGTGCAGAGAGAGTATTTGTTCCGAAGAGAGGAAAGGTTAATTACGAGTGTCTGGCTCTCGGGGACCTACCAGAACTTCGGCATTTTTTTGCAAGGATTGTACTTGATACTGTCTACCCTCTTGATCTCAATCATACTCGAGAATAAATTTTCGAGATAATTAGTAAATGGATGATTTTTGGGTCCTTTACTACAGGTATCTGACTTAAGCTTTGAGTATGTTTTGTACGCGGCCTGAGGTCAGGTTCATTGGATCATCTATTGAATCTCATAGAAGTTCAGATTTTTGTAATCTTTAATCCGGTATCTAGTAATACAGAAAGAAAAGGGAATGATGCAATCGGCAGTCGAATCCTACAAAAAGTGCTTTAGCGTCAAAAGCCTCAATTTTATGGTCCTAAGTATAATTAGAATGATTTCCAAGTCTTTGGAGTTACAGGCATCCGCATTTTT

Coding sequence (CDS)

ACCTTATCTCCCTCGACGGACACCACTCTTCCGTTTCCTCCGCAAGGTTACGAAGTTTACCTTGTAGGAGGTTGTGTTCGCGATCTTATCTTGAACAGAACACCAAAGGATTTTGACATAATAACTTCAGCTGAGCTTAAGGAGGTTTCAAGAACATTTTCATGGTGTGAAATAGTTGGGAGGAAGTTTCCTATATGCCATGTGCATATTGATGGTAGCATTGTAGAGGTGTCAAGTTTTAGCACATCCAGTAGGCCATTTGACAGACACTTGAACTCTGCTATTGAAAAGCCAATGAACTGTGAAGAGGAAGATTATGTCCGATGGAAGAATTGCATGCAACGTGACTTTACCATTAACGGGCTGATGTATGATCCATACAACAGTGCAGTATACGACTACTTGGAAGGAATGGAGGATATAAAACAAGCTAAACATAGATACGAACAGTGGTTCCTGCCTGCACTTCCTTTCAAGAGGATTGTGGTGAGTTTTCATTTGCAGAACTCCCTTATCGCAAGGCTTCAGATGGAAATGAACTATTTGTTGTCTTATGGTTCTGCTGAAGCTTCTTTGAGGTTGTTATGGAAATATGGACTTCTAGAAATACTTCTTCCAATTCAGGCAGCATATTTCATCCAATATGGTTTTCGGAGGTCCGATAAGGGATCAAATATGCTTTTGTCCCTATTTTCCAGCATGGATAAACTTCTGGCACCTAATAGACCTTGTCACAGTAGTTTATGTGAAGCAGTCTCAAACACTGTTTTCTCCTACAGGGTTGCAGTATTAGCCTTCCATGTTGCCCTGTCTGACCAGCCTAGGAGTCCTTTAGTGGTTGCAGCATTTAGTCTTGCAGTCCACAATGGTGGCAATATGATGGAAGCAATTAGCATTGCTAAGAGCATCAGTAGAGCACATAATGTAAACTTTCACGAATTGTTAGAACCTGAGAATCTGGAGGTCCAAACTTTGATTGATGAGGTTATGGATCTTACTACGTCTATTAAGAACGCACTTCATAAGATGACCGACGAGCACTCTGTGTCTCTAGTCTTGGAAATGTATCCCCAAGCACCAGCCTCGGATCTAGTTTTTATCCCATTGGTGGTGTACTTGAAGGTACGCAAAATTTTTGAGTGCGTTGTTGAGGGTGCAGAGAGAGTATTTGTTCCGAAGAGAGGAAAGGTTAATTACGAGTGTCTGGCTCTCGGGGACCTACCAGAACTTCGGCATTTTTTTGCAAGGATTGTACTTGATACTGTCTACCCTCTTGATCTCAATCATACTCGAGAATAA

Protein sequence

TLSPSTDTTLPFPPQGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSIVEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKHRYEQWFLPALPFKRIVVSFHLQNSLIARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPELRHFFARIVLDTVYPLDLNHTRE
Homology
BLAST of Carg10251 vs. NCBI nr
Match: KAG7023035.1 (pcnB, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 869.8 bits (2246), Expect = 1.0e-248
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   TLSPSTDTTLPFPPQGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVG 60
           TLSPSTDTTLPFPPQGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVG
Sbjct: 1   TLSPSTDTTLPFPPQGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVG 60

Query: 61  RKFPICHVHIDGSIVEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTIN 120
           RKFPICHVHIDGSIVEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTIN
Sbjct: 61  RKFPICHVHIDGSIVEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTIN 120

Query: 121 GLMYDPYNSAVYDYLEGMEDIKQAKHRYEQWFLPALPFKRIVVSFHLQNSLIARLQMEMN 180
           GLMYDPYNSAVYDYLEGMEDIKQAKHRYEQWFLPALPFKRIVVSFHLQNSLIARLQMEMN
Sbjct: 121 GLMYDPYNSAVYDYLEGMEDIKQAKHRYEQWFLPALPFKRIVVSFHLQNSLIARLQMEMN 180

Query: 181 YLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAP 240
           YLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAP
Sbjct: 181 YLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAP 240

Query: 241 NRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIA 300
           NRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIA
Sbjct: 241 NRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIA 300

Query: 301 KSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAP 360
           KSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAP
Sbjct: 301 KSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAP 360

Query: 361 ASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPELRHFFARIVLD 420
           ASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPELRHFFARIVLD
Sbjct: 361 ASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPELRHFFARIVLD 420

Query: 421 TVYPLDLNHTRE 433
           TVYPLDLNHTRE
Sbjct: 421 TVYPLDLNHTRE 432

BLAST of Carg10251 vs. NCBI nr
Match: XP_023516569.1 (uncharacterized protein LOC111780413 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761.1 bits (1964), Expect = 5.0e-216
Identity = 392/443 (88.49%), Postives = 401/443 (90.52%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI
Sbjct: 96  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 155

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNC+QRDFTINGLMYDPYNSAVYDY
Sbjct: 156 VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDY 215

Query: 135 LEGMEDIKQAKHR--------YEQWFLPALPFKRIVV------------SFHLQNSLI-- 194
           LEGMEDIKQAK R        +++     L   R+              S    + L+  
Sbjct: 216 LEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVST 275

Query: 195 ---ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
              ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS
Sbjct: 276 LNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 335

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LFSSMDKLLAPNRPCHSS+CEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN
Sbjct: 336 LFSSMDKLLAPNRPCHSSICEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 395

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 374
           GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV
Sbjct: 396 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 455

Query: 375 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPE 433
           SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPE
Sbjct: 456 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPE 515

BLAST of Carg10251 vs. NCBI nr
Match: XP_022921610.1 (uncharacterized protein LOC111429822 isoform X3 [Cucurbita moschata])

HSP 1 Score: 761.1 bits (1964), Expect = 5.0e-216
Identity = 393/443 (88.71%), Postives = 400/443 (90.29%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI
Sbjct: 95  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 154

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY
Sbjct: 155 VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 214

Query: 135 LEGMEDIKQAKHR--------YEQWFLPALPFKRIVV------------SFHLQNSLI-- 194
           LEGMEDIKQAK R        +++     L   R+              S    + L+  
Sbjct: 215 LEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVST 274

Query: 195 ---ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
              ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS
Sbjct: 275 LNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 334

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN
Sbjct: 335 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 394

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 374
           GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV
Sbjct: 395 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 454

Query: 375 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPE 433
           SLVLEMYPQAPASDLVFIPLVVYLKVRKIFEC VEGAERVFVPKRGKVNYECLALGDLPE
Sbjct: 455 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECFVEGAERVFVPKRGKVNYECLALGDLPE 514

BLAST of Carg10251 vs. NCBI nr
Match: XP_022921612.1 (uncharacterized protein LOC111429822 isoform X5 [Cucurbita moschata])

HSP 1 Score: 749.6 bits (1934), Expect = 1.5e-212
Identity = 393/462 (85.06%), Postives = 400/462 (86.58%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI
Sbjct: 16  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 75

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING------------- 134
           VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING             
Sbjct: 76  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGWFTRSCILRIHVP 135

Query: 135 ------LMYDPYNSAVYDYLEGMEDIKQAKHR--------YEQWFLPALPFKRIVV---- 194
                 LMYDPYNSAVYDYLEGMEDIKQAK R        +++     L   R+      
Sbjct: 136 ISIIQLLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRF 195

Query: 195 --------SFHLQNSLI-----ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 254
                   S    + L+     ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA
Sbjct: 196 NFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 255

Query: 255 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 314
           YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL
Sbjct: 256 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 315

Query: 315 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 374
           SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM
Sbjct: 316 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 375

Query: 375 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVF 433
           DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFEC VEGAERVF
Sbjct: 376 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECFVEGAERVF 435

BLAST of Carg10251 vs. NCBI nr
Match: XP_023516571.1 (uncharacterized protein LOC111780413 isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749.6 bits (1934), Expect = 1.5e-212
Identity = 392/462 (84.85%), Postives = 401/462 (86.80%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI
Sbjct: 16  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 75

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING------------- 134
           VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNC+QRDFTING             
Sbjct: 76  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCLQRDFTINGWFTRSCILRIHVP 135

Query: 135 ------LMYDPYNSAVYDYLEGMEDIKQAKHR--------YEQWFLPALPFKRIVV---- 194
                 LMYDPYNSAVYDYLEGMEDIKQAK R        +++     L   R+      
Sbjct: 136 ISIIQLLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRF 195

Query: 195 --------SFHLQNSLI-----ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 254
                   S    + L+     ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA
Sbjct: 196 NFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 255

Query: 255 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 314
           YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSS+CEAVSNTVFSYRVAVLAFHVAL
Sbjct: 256 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSICEAVSNTVFSYRVAVLAFHVAL 315

Query: 315 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 374
           SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM
Sbjct: 316 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 375

Query: 375 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVF 433
           DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVF
Sbjct: 376 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVF 435

BLAST of Carg10251 vs. ExPASy Swiss-Prot
Match: P44439 (Poly(A) polymerase I OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=pcnB PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.2e-25
Identity = 79/254 (31.10%), Postives = 127/254 (50.00%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFS-WCEIVGRKFPICHVHIDGS 74
           QG+E Y+VGGC+RDL+L + PKDFD+ T+A  +++   F   C +VGR+F + H+     
Sbjct: 46  QGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQIQNIFQRQCRLVGRRFRLAHIMFGRD 105

Query: 75  IVEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRW---KNCMQRDFTINGLMYDPYNSA 134
           I+EV++F  ++    R+ N A +        D V     ++  +RDFT+N L Y+P ++ 
Sbjct: 106 IIEVATF-RANHSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNPQDNT 165

Query: 135 VYDYLEGMEDIKQAK--------HRYEQWFLPALPFKRIVVSFH---------------- 194
           + DY EG++D+K  K         RY++  +  L   R +                    
Sbjct: 166 LRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAP 225

Query: 195 -LQNSLIARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSN 240
            L+N   ARL  E   LL  G    + RLL +YGL E L P  +AYF +   +       
Sbjct: 226 LLKNIPPARLFDESLKLLQAGQGVKTYRLLRQYGLFEQLFPALSAYFTE---KEDSFAER 285

BLAST of Carg10251 vs. ExPASy Swiss-Prot
Match: Q8Z9C3 (Poly(A) polymerase I OS=Salmonella typhi OX=90370 GN=pcnB PE=3 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 9.1e-19
Identity = 50/131 (38.17%), Postives = 75/131 (57.25%), Query Frame = 0

Query: 16  GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSIV 75
           GYE YLVGG VRDL+L + PKDFD+ T+A   +V + F  C +VGR+F + HV     I+
Sbjct: 59  GYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEII 118

Query: 76  EVSSF--STSSRPFDRHLNSAIEKPMNCEEEDYVR-WKNCMQRDFTINGLMYDPYNSAVY 135
           EV++F         DR  +   +  M   +  +    ++  +RDFTIN L Y   +  V 
Sbjct: 119 EVATFRGHNEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVR 178

Query: 136 DYLEGMEDIKQ 144
           DY+ GM+D+++
Sbjct: 179 DYVGGMQDLQE 189

BLAST of Carg10251 vs. ExPASy Swiss-Prot
Match: Q8ZRQ8 (Poly(A) polymerase I OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=pcnB PE=3 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 1.6e-18
Identity = 53/137 (38.69%), Postives = 76/137 (55.47%), Query Frame = 0

Query: 16  GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSIV 75
           GYE YLVGG VRDL+L + PKDFD+ T+A   +V + F  C +VGR+F + HV     I+
Sbjct: 59  GYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEII 118

Query: 76  EVSSF------STSSRPFDRHLNSAI---EKPMNCEEEDYVRWKNCMQRDFTINGLMYDP 135
           EV++F      S S R   +   + +   +      EED        +RDFTIN L Y  
Sbjct: 119 EVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSIEED------AQRRDFTINSLYYSV 178

Query: 136 YNSAVYDYLEGMEDIKQ 144
            +  V DY+ GM+D+++
Sbjct: 179 ADFTVRDYVGGMQDLQE 189

BLAST of Carg10251 vs. ExPASy Swiss-Prot
Match: P0ABF3 (Poly(A) polymerase I OS=Escherichia coli O157:H7 OX=83334 GN=pcnB PE=3 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 2.7e-18
Identity = 50/130 (38.46%), Postives = 75/130 (57.69%), Query Frame = 0

Query: 16  GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSIV 75
           GYE +LVGG VRDL+L + PKDFD+ T+A  ++V + F  C +VGR+F + HV     I+
Sbjct: 59  GYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEII 118

Query: 76  EVSSF--STSSRPFDRHLNSAIEKPMNCEEEDYVR-WKNCMQRDFTINGLMYDPYNSAVY 135
           EV++F         DR  +   +  M   +  +    ++  +RDFTIN L Y   +  V 
Sbjct: 119 EVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVR 178

Query: 136 DYLEGMEDIK 143
           DY+ GM+D+K
Sbjct: 179 DYVGGMKDLK 188

BLAST of Carg10251 vs. ExPASy Swiss-Prot
Match: P0ABF2 (Poly(A) polymerase I OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=pcnB PE=3 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 2.7e-18
Identity = 50/130 (38.46%), Postives = 75/130 (57.69%), Query Frame = 0

Query: 16  GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSIV 75
           GYE +LVGG VRDL+L + PKDFD+ T+A  ++V + F  C +VGR+F + HV     I+
Sbjct: 59  GYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEII 118

Query: 76  EVSSF--STSSRPFDRHLNSAIEKPMNCEEEDYVR-WKNCMQRDFTINGLMYDPYNSAVY 135
           EV++F         DR  +   +  M   +  +    ++  +RDFTIN L Y   +  V 
Sbjct: 119 EVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVR 178

Query: 136 DYLEGMEDIK 143
           DY+ GM+D+K
Sbjct: 179 DYVGGMKDLK 188

BLAST of Carg10251 vs. ExPASy TrEMBL
Match: A0A6J1E6A2 (uncharacterized protein LOC111429822 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111429822 PE=3 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.4e-216
Identity = 393/443 (88.71%), Postives = 400/443 (90.29%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI
Sbjct: 95  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 154

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY
Sbjct: 155 VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 214

Query: 135 LEGMEDIKQAKHR--------YEQWFLPALPFKRIVV------------SFHLQNSLI-- 194
           LEGMEDIKQAK R        +++     L   R+              S    + L+  
Sbjct: 215 LEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVST 274

Query: 195 ---ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
              ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS
Sbjct: 275 LNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 334

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN
Sbjct: 335 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 394

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 374
           GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV
Sbjct: 395 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 454

Query: 375 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPE 433
           SLVLEMYPQAPASDLVFIPLVVYLKVRKIFEC VEGAERVFVPKRGKVNYECLALGDLPE
Sbjct: 455 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECFVEGAERVFVPKRGKVNYECLALGDLPE 514

BLAST of Carg10251 vs. ExPASy TrEMBL
Match: A0A6J1E4D0 (uncharacterized protein LOC111429822 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429822 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 7.3e-213
Identity = 393/462 (85.06%), Postives = 400/462 (86.58%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI
Sbjct: 95  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 154

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING------------- 134
           VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING             
Sbjct: 155 VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGWFTRSCILRIHVP 214

Query: 135 ------LMYDPYNSAVYDYLEGMEDIKQAKHR--------YEQWFLPALPFKRIVV---- 194
                 LMYDPYNSAVYDYLEGMEDIKQAK R        +++     L   R+      
Sbjct: 215 ISIIQLLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRF 274

Query: 195 --------SFHLQNSLI-----ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 254
                   S    + L+     ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA
Sbjct: 275 NFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 334

Query: 255 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 314
           YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL
Sbjct: 335 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 394

Query: 315 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 374
           SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM
Sbjct: 395 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 454

Query: 375 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVF 433
           DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFEC VEGAERVF
Sbjct: 455 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECFVEGAERVF 514

BLAST of Carg10251 vs. ExPASy TrEMBL
Match: A0A6J1E0Z8 (uncharacterized protein LOC111429822 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111429822 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 7.3e-213
Identity = 393/462 (85.06%), Postives = 400/462 (86.58%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI
Sbjct: 16  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 75

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING------------- 134
           VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING             
Sbjct: 76  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGWFTRSCILRIHVP 135

Query: 135 ------LMYDPYNSAVYDYLEGMEDIKQAKHR--------YEQWFLPALPFKRIVV---- 194
                 LMYDPYNSAVYDYLEGMEDIKQAK R        +++     L   R+      
Sbjct: 136 ISIIQLLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRF 195

Query: 195 --------SFHLQNSLI-----ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 254
                   S    + L+     ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA
Sbjct: 196 NFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 255

Query: 255 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 314
           YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL
Sbjct: 256 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 315

Query: 315 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 374
           SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM
Sbjct: 316 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 375

Query: 375 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVF 433
           DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFEC VEGAERVF
Sbjct: 376 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECFVEGAERVF 435

BLAST of Carg10251 vs. ExPASy TrEMBL
Match: A0A6J1JI15 (uncharacterized protein LOC111484780 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111484780 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.1e-211
Identity = 386/443 (87.13%), Postives = 396/443 (89.39%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVG KFPICHVHIDGSI
Sbjct: 96  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSI 155

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           VEVSSFSTSSRPFDRHL+SAIEKPMNCEEEDYVRWKNC+QRDFTINGLMYDPYNSAVYDY
Sbjct: 156 VEVSSFSTSSRPFDRHLDSAIEKPMNCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDY 215

Query: 135 LEGMEDIKQAKHR--------YEQWFLPALPFKRIVV------------SFHLQNSLI-- 194
           LEGMEDIKQAK R        +++     L   R+              S    + L+  
Sbjct: 216 LEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVST 275

Query: 195 ---ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
              ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS
Sbjct: 276 LNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 335

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN
Sbjct: 336 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 395

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 374
           GGNMMEAISIAKSI RAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV
Sbjct: 396 GGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 455

Query: 375 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKRGKVNYECLALGDLPE 433
           SLVLEMYPQAPAS LVFIPLVVYLKVRKIFECVVEGAE VFVPKRGKVNY+CLALGDLPE
Sbjct: 456 SLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPE 515

BLAST of Carg10251 vs. ExPASy TrEMBL
Match: A0A6J1J9J1 (uncharacterized protein LOC111484780 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484780 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.2e-208
Identity = 386/462 (83.55%), Postives = 396/462 (85.71%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVG KFPICHVHIDGSI
Sbjct: 96  KGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSI 155

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTING------------- 134
           VEVSSFSTSSRPFDRHL+SAIEKPMNCEEEDYVRWKNC+QRDFTING             
Sbjct: 156 VEVSSFSTSSRPFDRHLDSAIEKPMNCEEEDYVRWKNCLQRDFTINGWLTRFCILRIHVP 215

Query: 135 ------LMYDPYNSAVYDYLEGMEDIKQAKHR--------YEQWFLPALPFKRIVV---- 194
                 LMYDPYNSAVYDYLEGMEDIKQAK R        +++     L   R+      
Sbjct: 216 ISIIQLLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRF 275

Query: 195 --------SFHLQNSLI-----ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 254
                   S    + L+     ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA
Sbjct: 276 NFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAA 335

Query: 255 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 314
           YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL
Sbjct: 336 YFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVAL 395

Query: 315 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVM 374
           SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSI RAHNVNFHELLEPENLEVQTLIDEVM
Sbjct: 396 SDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVM 455

Query: 375 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVF 433
           DLTTSIKNALHKMTDEHSVSLVLEMYPQAPAS LVFIPLVVYLKVRKIFECVVEGAE VF
Sbjct: 456 DLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVF 515

BLAST of Carg10251 vs. TAIR 10
Match: AT5G23690.1 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 456.1 bits (1172), Expect = 3.2e-128
Identity = 247/443 (55.76%), Postives = 316/443 (71.33%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +G++VYLVGGCVRDLIL RTPKDFDI+TSAEL+EV RTF  CEIVGR+FPICHVHI   +
Sbjct: 93  KGHDVYLVGGCVRDLILKRTPKDFDILTSAELREVVRTFPRCEIVGRRFPICHVHIGDDL 152

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCE-EEDYVRWKNCMQRDFTINGLMYDPYNSAVYD 134
           +EVSSFSTS++   R+  +  ++    + +ED +R  NC+QRDFTINGLM+DPY   VYD
Sbjct: 153 IEVSSFSTSAQNSSRNTRTECKESSGSDGDEDCIRLNNCLQRDFTINGLMFDPYAKVVYD 212

Query: 135 YLEGMEDIKQAKHR--------YEQWFLPALPFKRIV-----------------VSFHLQ 194
           YL GMEDI++AK R        + Q     L   RI                  +S  +Q
Sbjct: 213 YLGGMEDIRKAKVRTVIHAGTSFHQDCARILRAIRIAARLGFRMSKETAHFIKNLSLLVQ 272

Query: 195 NSLIARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLL 254
                R+ MEMNY+L+YGSAEASLRLLWK+G+LEILLPIQAAY  + GFRR DK +NMLL
Sbjct: 273 RLDKGRILMEMNYMLAYGSAEASLRLLWKFGILEILLPIQAAYLARSGFRRRDKRTNMLL 332

Query: 255 SLFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVH 314
           SLF+++DKLLAP+RPCHSSL            +A+LAFH AL+D+PRSP+VVAAFSLAVH
Sbjct: 333 SLFANLDKLLAPDRPCHSSLW-----------IAILAFHKALADKPRSPIVVAAFSLAVH 392

Query: 315 NGGNMMEAISIAKSISRAHNVNFHELLEP-ENLEVQTLIDEVMDLTTSIKNALHKMTDEH 374
           N G+++EA+ I K I+R H+ +F EL+EP ENL+ QTL+DEVMDL  SI++AL++MTD +
Sbjct: 393 NCGDILEAVEITKKITRPHDKSFFELVEPEENLDFQTLLDEVMDLDASIEDALNQMTDAY 452

Query: 375 SVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERV-FVPKRG-KVNYECLALG 429
            +S  +  YPQAP SDLVFIPL +YL+  +IF+CV     R+ F  K+G K+ Y  L  G
Sbjct: 453 FISKAMSAYPQAPYSDLVFIPLQLYLRAGRIFDCVKNEETRIGFEAKQGSKIEYGSLNSG 512

BLAST of Carg10251 vs. TAIR 10
Match: AT3G48830.1 (polynucleotide adenylyltransferase family protein / RNA recognition motif (RRM)-containing protein )

HSP 1 Score: 428.3 bits (1100), Expect = 7.2e-120
Identity = 223/405 (55.06%), Postives = 288/405 (71.11%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +GY+VYLVGGCVRDLIL RTPKDFDI+TSAEL+EV R+FS CEI+G+KFPICHVHI   +
Sbjct: 90  KGYDVYLVGGCVRDLILKRTPKDFDILTSAELREVVRSFSRCEIIGKKFPICHVHIGNDM 149

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           +EVSSFSTS++   R+  +   K     +ED +R+ NC+QRDFTINGLM+DPY   +YDY
Sbjct: 150 IEVSSFSTSAQNSLRNTRTGSGKSNGSYDEDSIRFNNCLQRDFTINGLMFDPYAKVIYDY 209

Query: 135 LEGMEDIKQAKHR--------YEQWFLPALPFKRIV-----------------VSFHLQN 194
           L G+EDIK+AK R        +++     L   RI                  +SF +Q 
Sbjct: 210 LGGIEDIKKAKVRTVFHAGTSFQEDSARILRGTRIAARLGFTISKETAHFLKNLSFLVQR 269

Query: 195 SLIARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
               R+ +EMNY+L+YGSAEASLRLLWK+G+LEILLPIQAAY ++ GF+R DK SN+LLS
Sbjct: 270 LHRGRILLEMNYMLAYGSAEASLRLLWKFGILEILLPIQAAYLVRTGFKRRDKRSNLLLS 329

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LF ++DKLLAP++PCHSSL            + +LA H AL+DQPR P VVAAFSLAVHN
Sbjct: 330 LFGNLDKLLAPDKPCHSSLW-----------LTILALHKALADQPRYPSVVAAFSLAVHN 389

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSV 374
           GG+++EA+   + +++ HN +F ELLEPE ++ QTL+DEVMD  +SIK AL +MTD   +
Sbjct: 390 GGDVLEAVKNTRKVTKPHNRSFFELLEPEEMDSQTLLDEVMDFDSSIKEALGQMTDGRFI 449

Query: 375 SLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEGAERVFVPKR 395
           S  +  YPQAP SD+VFIPL +YL  R+IFECV E  ++ FVPK+
Sbjct: 450 SKAMAAYPQAPYSDMVFIPLQLYLDARRIFECVKENGQKGFVPKQ 483

BLAST of Carg10251 vs. TAIR 10
Match: AT1G28090.1 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.2e-108
Identity = 212/442 (47.96%), Postives = 293/442 (66.29%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +G++VYLVGGCVRDLIL+R PKDFD+IT+AELKEV + F  C+IVGR+FPICHV++D  I
Sbjct: 111 KGFQVYLVGGCVRDLILDRIPKDFDVITTAELKEVRKVFPGCQIVGRRFPICHVYVDDII 170

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           +EVSSFSTS+R   +  N +  +P  C+E DY+RWKNC+QRDFT+NGLM+DP  + VYDY
Sbjct: 171 IEVSSFSTSART-GKAPNKSFRRPAGCDERDYIRWKNCLQRDFTVNGLMFDPSENVVYDY 230

Query: 135 LEGMEDIKQAKHRY-----------EQWFLPALPF---------KRIVVSF-HLQNSLI- 194
           + G+ED++ +K R                L A+           K + +S   L +SL+ 
Sbjct: 231 IGGVEDLRNSKVRTVSAANLSFVEDTARILRAIRIAARLGFSLTKDVAISVKELSSSLLR 290

Query: 195 ---ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
              +R++ME+NY+L+YGSAEASLRLLW++GL+EILLPIQA+Y +  GFRR D  SNMLLS
Sbjct: 291 LDPSRIRMEINYMLAYGSAEASLRLLWRFGLMEILLPIQASYLVSQGFRRRDGRSNMLLS 350

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LF ++D+L+AP+RPC   L            + +LAFH AL DQPR P VVA+F LA+++
Sbjct: 351 LFRNLDRLVAPDRPCSEFLW-----------IGILAFHKALVDQPRDPTVVASFCLAIYS 410

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQT---LIDEVMDLTTSIKNALHKMTDE 374
             ++ EAI+IA+S S+ HN +F EL  PE     +   +  +V+ L  SI++A  K+ + 
Sbjct: 411 EVSLSEAIAIARSNSKQHNSHFQELSSPEKDTADSESKISQQVIKLAESIRSAARKLNNR 470

Query: 375 HSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVE--GAERVFVPK-RGKVNYECLA 426
             ++  +  YPQAP SD+VF+  ++  +V K+F  V      ER  VP    ++NY+ LA
Sbjct: 471 DYIANAMSKYPQAPGSDMVFLSRLMLERVEKMFGNVRRKGNQERDDVPSLERRINYKSLA 530

BLAST of Carg10251 vs. TAIR 10
Match: AT1G28090.2 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.2e-108
Identity = 212/442 (47.96%), Postives = 293/442 (66.29%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +G++VYLVGGCVRDLIL+R PKDFD+IT+AELKEV + F  C+IVGR+FPICHV++D  I
Sbjct: 75  KGFQVYLVGGCVRDLILDRIPKDFDVITTAELKEVRKVFPGCQIVGRRFPICHVYVDDII 134

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           +EVSSFSTS+R   +  N +  +P  C+E DY+RWKNC+QRDFT+NGLM+DP  + VYDY
Sbjct: 135 IEVSSFSTSART-GKAPNKSFRRPAGCDERDYIRWKNCLQRDFTVNGLMFDPSENVVYDY 194

Query: 135 LEGMEDIKQAKHRY-----------EQWFLPALPF---------KRIVVSF-HLQNSLI- 194
           + G+ED++ +K R                L A+           K + +S   L +SL+ 
Sbjct: 195 IGGVEDLRNSKVRTVSAANLSFVEDTARILRAIRIAARLGFSLTKDVAISVKELSSSLLR 254

Query: 195 ---ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
              +R++ME+NY+L+YGSAEASLRLLW++GL+EILLPIQA+Y +  GFRR D  SNMLLS
Sbjct: 255 LDPSRIRMEINYMLAYGSAEASLRLLWRFGLMEILLPIQASYLVSQGFRRRDGRSNMLLS 314

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LF ++D+L+AP+RPC   L            + +LAFH AL DQPR P VVA+F LA+++
Sbjct: 315 LFRNLDRLVAPDRPCSEFLW-----------IGILAFHKALVDQPRDPTVVASFCLAIYS 374

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQT---LIDEVMDLTTSIKNALHKMTDE 374
             ++ EAI+IA+S S+ HN +F EL  PE     +   +  +V+ L  SI++A  K+ + 
Sbjct: 375 EVSLSEAIAIARSNSKQHNSHFQELSSPEKDTADSESKISQQVIKLAESIRSAARKLNNR 434

Query: 375 HSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVE--GAERVFVPK-RGKVNYECLA 426
             ++  +  YPQAP SD+VF+  ++  +V K+F  V      ER  VP    ++NY+ LA
Sbjct: 435 DYIANAMSKYPQAPGSDMVFLSRLMLERVEKMFGNVRRKGNQERDDVPSLERRINYKSLA 494

BLAST of Carg10251 vs. TAIR 10
Match: AT1G28090.3 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.2e-108
Identity = 212/442 (47.96%), Postives = 293/442 (66.29%), Query Frame = 0

Query: 15  QGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSI 74
           +G++VYLVGGCVRDLIL+R PKDFD+IT+AELKEV + F  C+IVGR+FPICHV++D  I
Sbjct: 48  KGFQVYLVGGCVRDLILDRIPKDFDVITTAELKEVRKVFPGCQIVGRRFPICHVYVDDII 107

Query: 75  VEVSSFSTSSRPFDRHLNSAIEKPMNCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDY 134
           +EVSSFSTS+R   +  N +  +P  C+E DY+RWKNC+QRDFT+NGLM+DP  + VYDY
Sbjct: 108 IEVSSFSTSART-GKAPNKSFRRPAGCDERDYIRWKNCLQRDFTVNGLMFDPSENVVYDY 167

Query: 135 LEGMEDIKQAKHRY-----------EQWFLPALPF---------KRIVVSF-HLQNSLI- 194
           + G+ED++ +K R                L A+           K + +S   L +SL+ 
Sbjct: 168 IGGVEDLRNSKVRTVSAANLSFVEDTARILRAIRIAARLGFSLTKDVAISVKELSSSLLR 227

Query: 195 ---ARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLS 254
              +R++ME+NY+L+YGSAEASLRLLW++GL+EILLPIQA+Y +  GFRR D  SNMLLS
Sbjct: 228 LDPSRIRMEINYMLAYGSAEASLRLLWRFGLMEILLPIQASYLVSQGFRRRDGRSNMLLS 287

Query: 255 LFSSMDKLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHN 314
           LF ++D+L+AP+RPC   L            + +LAFH AL DQPR P VVA+F LA+++
Sbjct: 288 LFRNLDRLVAPDRPCSEFLW-----------IGILAFHKALVDQPRDPTVVASFCLAIYS 347

Query: 315 GGNMMEAISIAKSISRAHNVNFHELLEPENLEVQT---LIDEVMDLTTSIKNALHKMTDE 374
             ++ EAI+IA+S S+ HN +F EL  PE     +   +  +V+ L  SI++A  K+ + 
Sbjct: 348 EVSLSEAIAIARSNSKQHNSHFQELSSPEKDTADSESKISQQVIKLAESIRSAARKLNNR 407

Query: 375 HSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVE--GAERVFVPK-RGKVNYECLA 426
             ++  +  YPQAP SD+VF+  ++  +V K+F  V      ER  VP    ++NY+ LA
Sbjct: 408 DYIANAMSKYPQAPGSDMVFLSRLMLERVEKMFGNVRRKGNQERDDVPSLERRINYKSLA 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023035.11.0e-248100.00pcnB, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023516569.15.0e-21688.49uncharacterized protein LOC111780413 isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_022921610.15.0e-21688.71uncharacterized protein LOC111429822 isoform X3 [Cucurbita moschata][more]
XP_022921612.11.5e-21285.06uncharacterized protein LOC111429822 isoform X5 [Cucurbita moschata][more]
XP_023516571.11.5e-21284.85uncharacterized protein LOC111780413 isoform X5 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P444392.2e-2531.10Poly(A) polymerase I OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / ... [more]
Q8Z9C39.1e-1938.17Poly(A) polymerase I OS=Salmonella typhi OX=90370 GN=pcnB PE=3 SV=2[more]
Q8ZRQ81.6e-1838.69Poly(A) polymerase I OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700... [more]
P0ABF32.7e-1838.46Poly(A) polymerase I OS=Escherichia coli O157:H7 OX=83334 GN=pcnB PE=3 SV=2[more]
P0ABF22.7e-1838.46Poly(A) polymerase I OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UP... [more]
Match NameE-valueIdentityDescription
A0A6J1E6A22.4e-21688.71uncharacterized protein LOC111429822 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1E4D07.3e-21385.06uncharacterized protein LOC111429822 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1E0Z87.3e-21385.06uncharacterized protein LOC111429822 isoform X5 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JI151.1e-21187.13uncharacterized protein LOC111484780 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J9J13.2e-20883.55uncharacterized protein LOC111484780 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G23690.13.2e-12855.76Polynucleotide adenylyltransferase family protein [more]
AT3G48830.17.2e-12055.06polynucleotide adenylyltransferase family protein / RNA recognition motif (RRM)-... [more]
AT1G28090.12.2e-10847.96Polynucleotide adenylyltransferase family protein [more]
AT1G28090.22.2e-10847.96Polynucleotide adenylyltransferase family protein [more]
AT1G28090.32.2e-10847.96Polynucleotide adenylyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032828tRNA nucleotidyltransferase/poly(A) polymerase, RNA and SrmB- binding domainPFAMPF12627PolyA_pol_RNAbdcoord: 167..210
e-value: 1.1E-9
score: 37.9
NoneNo IPR availableGENE3D1.10.3090.10coord: 164..349
e-value: 4.0E-7
score: 31.9
NoneNo IPR availablePANTHERPTHR43051POLYNUCLEOTIDE ADENYLYLTRANSFERASE FAMILY PROTEINcoord: 15..147
coord: 173..426
NoneNo IPR availablePANTHERPTHR43051:SF2POLYNUCLEOTIDE ADENYLYLTRANSFERASE FAMILY PROTEIN-RELATEDcoord: 173..426
coord: 15..147
NoneNo IPR availableSUPERFAMILY81891Poly A polymerase C-terminal region-likecoord: 166..248
IPR002646Poly A polymerase, head domainPFAMPF01743PolyA_polcoord: 19..144
e-value: 4.7E-24
score: 85.2
IPR002646Poly A polymerase, head domainCDDcd05398NT_ClassII-CCAasecoord: 16..143
e-value: 1.63396E-33
score: 120.775
IPR043519Nucleotidyltransferase superfamilyGENE3D3.30.460.10Beta Polymerase, domain 2coord: 11..148
e-value: 1.1E-36
score: 127.8
IPR043519Nucleotidyltransferase superfamilySUPERFAMILY81301Nucleotidyltransferasecoord: 12..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10251-RACarg10251-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001680 tRNA 3'-terminal CCA addition
biological_process GO:0006396 RNA processing
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0003723 RNA binding