Carg10149 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10149
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Serine hydroxymethyltransferase
Location	Carg_Chr17: 480322 .. 482739 (-)
RNA-Seq Expression	Carg10149
Synteny	Carg10149

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTGCCCCCATTTTTCAGCTTCTTCTTCTTCTTCTACTCAAATTTCTCTGTAATTTCTCCTTTCTCGCCATGGATCCCGTCAATACTTGGGGCAATACTCCTCTCGTAAACGTTGATCCTGAGATCTTCGATTTGATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACCTCCTTCGCCGTCATCGAGGCCCTCGGAAGTGCCTTGACCAACAAATACTCCGAGGGCATGCCGGGGAACCGATACTACGGTGGAAACGAGTTCATCGATGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCTTTCCATGTCGATCCGGCCAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATAATGGGGCTAGATCTTCCTTCTGGTGGTCATTTGACGCATGGTTACTACACCTCCGGAGGGAAAAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCGCCACTGGATTTATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGAAATTGCTGATAAATGCGGTGCCCTTCTTCTCTGTGATATGGCTCACATTAGTGGTCTCGTTGCTGCTCAGGTATATAATGAAACAAATCTATGAATTGAGTTCTATAACTTATCATTTTATCTTGCGAAAGTTGCCAATTGGATGTTGTTTTCCATTTCTGAATTTGGATCTGCTGGTTTATGTTGATTAGGAATAGTTAGAAATGGCACAGGTTTTCAGATCTAGAATTGTTTGAGCATCTTACGCAGCAGGATCTGGCATGCTAAGAAGCTTATTTAATGCCTAATTATCGATGCTGCATTGCTTCTTTGTAACAGAAGATAAATTATGTTCAATGGATTGACGGAACGGTTATGTTTTCAATTGTTTCTTACAAACATTTTGCATTCCTTGTTCTTATGATTAAGTTTTAAATGCTAGATTAAAATCGAAATGAAGATCTACTTTGAGAAAATGGATAGATTTCTGTCTCGTTTTGTTTTGAACTGATATTGTGATTGCTTATGATCTCTGTTTCTTTTCTTGCAACTTAAGCGAGTTGGGTCTGGCTGGTATTTTAAAATATTGATGTCCAATGATCGTTTGCAGGAAGCTGCGAACCCGTTCGAGTACTGTGACGTTGTGACGACCACGACTCACAAGAGTTTGAGGGGTCCTAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCCCCTAAGAAGGGTCAGCCCGAGAATGCTGTCTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCATCAAATTGGCGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGGTCTATGCGAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGCTACAAGCTTGTCACCGGAGGAACCGAGAACCATCTCGTTCTCTGGGATCTTCGTCCATTGGGATTGACCGGTAATTTTGTGGAATCAACCTAAGAAAACTAACAGGAAGAACATATTCTTTTTTGGTAATGTAACAATGTTGTTGTAATTCCTTTGGCAGGCAACAAGGTCGAGAAGCTTTGCGACTTGTGCAACATCACTGTAAACAAAAACGCCGTGTTTGGCGACAGCAGCGCACTGGCTCCTGGAGGTGTTAGAATCGGTAAGTTACCGATATCATAATCCACAGTCCTTAACTTGTTCTTCCTGTAATCAATCAATGAGTAGCAATTTCAAACACATGATTAACAAGACATCAAATTGTGATGGCAATCATTAGGTGCACCGGCCATGACTTCTCGAGGTTTGGTCGAGAAAGACTTCGAACAGATCGCTGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAACATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGCGCTGAAGGCGGACGTTGAGAAATTTTCTGGCTCGTTCGAGATGCCCGGTTTTCTCATGTCTGAGATGAAGTACAAGGACTAGACAGCAGACACAGACAACAAGATCTTCGGGAAAATTAATAAAGTTTTGGGTGTTGGTTGAATCTTTAAAATCATCTGCTTAAATTTGAAAAAGTAAAAAGGAAAGATTGCCGTTTGTTTGTTTGTTTGTTTTCCTTCTTTTACCAATTTGTAGACTTTAGATTTCCATTCTCAATATATTCATTCCAATATTTCCATTTTCGTATT

mRNA sequence

TCTTGCCCCCATTTTTCAGCTTCTTCTTCTTCTTCTACTCAAATTTCTCTGTAATTTCTCCTTTCTCGCCATGGATCCCGTCAATACTTGGGGCAATACTCCTCTCGTAAACGTTGATCCTGAGATCTTCGATTTGATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACCTCCTTCGCCGTCATCGAGGCCCTCGGAAGTGCCTTGACCAACAAATACTCCGAGGGCATGCCGGGGAACCGATACTACGGTGGAAACGAGTTCATCGATGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCTTTCCATGTCGATCCGGCCAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATAATGGGGCTAGATCTTCCTTCTGGTGGTCATTTGACGCATGGTTACTACACCTCCGGAGGGAAAAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCGCCACTGGATTTATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGAAATTGCTGATAAATGCGGTGCCCTTCTTCTCTGTGATATGGCTCACATTAGTGGTCTCGTTGCTGCTCAGGAAGCTGCGAACCCGTTCGAGTACTGTGACGTTGTGACGACCACGACTCACAAGAGTTTGAGGGGTCCTAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCCCCTAAGAAGGGTCAGCCCGAGAATGCTGTCTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCATCAAATTGGCGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGGTCTATGCGAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGCTACAAGCTTGTCACCGGAGGAACCGAGAACCATCTCGTTCTCTGGGATCTTCGTCCATTGGGATTGACCGGCAACAAGGTCGAGAAGCTTTGCGACTTGTGCAACATCACTGTAAACAAAAACGCCGTGTTTGGCGACAGCAGCGCACTGGCTCCTGGAGGTGTTAGAATCGGTGCACCGGCCATGACTTCTCGAGGTTTGGTCGAGAAAGACTTCGAACAGATCGCTGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAACATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGCGCTGAAGGCGGACGTTGAGAAATTTTCTGGCTCGTTCGAGATGCCCGGTTTTCTCATGTCTGAGATGAAGTACAAGGACTAGACAGCAGACACAGACAACAAGATCTTCGGGAAAATTAATAAAGTTTTGGGTGTTGGTTGAATCTTTAAAATCATCTGCTTAAATTTGAAAAAGTAAAAAGGAAAGATTGCCGTTTGTTTGTTTGTTTGTTTTCCTTCTTTTACCAATTTGTAGACTTTAGATTTCCATTCTCAATATATTCATTCCAATATTTCCATTTTCGTATT

Coding sequence (CDS)

ATGGATCCCGTCAATACTTGGGGCAATACTCCTCTCGTAAACGTTGATCCTGAGATCTTCGATTTGATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACCTCCTTCGCCGTCATCGAGGCCCTCGGAAGTGCCTTGACCAACAAATACTCCGAGGGCATGCCGGGGAACCGATACTACGGTGGAAACGAGTTCATCGATGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCTTTCCATGTCGATCCGGCCAAATGGGGCGTTAATGTCCAGCCCTACTCTGGTTCTCCGGCGAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATAATGGGGCTAGATCTTCCTTCTGGTGGTCATTTGACGCATGGTTACTACACCTCCGGAGGGAAAAAGATCTCCGCTACCTCGATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCGCCACTGGATTTATTGATTACGATAAGTTGGAGGAGAAGGCTTTGGATTTCAGGCCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGAAATTGCTGATAAATGCGGTGCCCTTCTTCTCTGTGATATGGCTCACATTAGTGGTCTCGTTGCTGCTCAGGAAGCTGCGAACCCGTTCGAGTACTGTGACGTTGTGACGACCACGACTCACAAGAGTTTGAGGGGTCCTAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCCCCTAAGAAGGGTCAGCCCGAGAATGCTGTCTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCATCAAATTGGCGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGGTCTATGCGAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAACAAGGGCTACAAGCTTGTCACCGGAGGAACCGAGAACCATCTCGTTCTCTGGGATCTTCGTCCATTGGGATTGACCGGCAACAAGGTCGAGAAGCTTTGCGACTTGTGCAACATCACTGTAAACAAAAACGCCGTGTTTGGCGACAGCAGCGCACTGGCTCCTGGAGGTGTTAGAATCGGTGCACCGGCCATGACTTCTCGAGGTTTGGTCGAGAAAGACTTCGAACAGATCGCTGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAATGTCCAGAAGGAACATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGAAATCGAAGCGCTGAAGGCGGACGTTGAGAAATTTTCTGGCTCGTTCGAGATGCCCGGTTTTCTCATGTCTGAGATGAAGTACAAGGACTAG

Protein sequence

MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD

Homology

[Close]

BLAST of Carg10149 vs. NCBI nr
Match: KAG7013425.1 (Serine hydroxymethyltransferase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 973.0 bits (2514), Expect = 9.2e-280
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. NCBI nr
Match: XP_022959093.1 (serine hydroxymethyltransferase 4-like [Cucurbita moschata] >KAG6574849.1 Serine hydroxymethyltransferase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 968.8 bits (2503), Expect = 1.7e-278
Identity = 469/471 (99.58%), Postives = 469/471 (99.58%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. NCBI nr
Match: XP_023548526.1 (serine hydroxymethyltransferase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 966.8 bits (2498), Expect = 6.6e-278
Identity = 468/471 (99.36%), Postives = 469/471 (99.58%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFE+CDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEHCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. NCBI nr
Match: XP_023006191.1 (serine hydroxymethyltransferase 4-like [Cucurbita maxima])

HSP 1 Score: 964.5 bits (2492), Expect = 3.3e-277
Identity = 466/471 (98.94%), Postives = 469/471 (99.58%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGS+LTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSSLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. NCBI nr
Match: XP_004138967.1 (serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical protein Csa_006275 [Cucumis sativus])

HSP 1 Score: 948.0 bits (2449), Expect = 3.2e-272
Identity = 457/471 (97.03%), Postives = 464/471 (98.51%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKE+GKLLKDFNKGLVNNKEIE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 2.0e-261
Identity = 432/471 (91.72%), Postives = 455/471 (96.60%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV++WGNT LV+VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+  TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFR IADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFKVYAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           ++QK +GKLLKDFNKGLVNNK+++ LKADVEKFS S+EMPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 2.7e-234
Identity = 381/468 (81.41%), Postives = 429/468 (91.67%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L  VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TG+IDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFR +ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FKVYAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMK 469
           ++Q+++GK++KDFNKGLVNNKEI+ +KADVE+F+  F+MPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

[Close]

BLAST of Carg10149 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 3.3e-179
Identity = 294/466 (63.09%), Postives = 369/466 (79.18%), Query Frame = 0


Query: 4   VNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WG+ P+   DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TG+IDYDKLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R+IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G        +  YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +K Y +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
           T  +Q+EHGK  K+F K L  NK+I  L+  VE F+  +EMP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

[Close]

BLAST of Carg10149 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 5.6e-179
Identity = 297/466 (63.73%), Postives = 368/466 (78.97%), Query Frame = 0


Query: 4   VNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TG+IDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         N  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K+Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
               Q+EHGKL K+  K + + KEI  L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

[Close]

BLAST of Carg10149 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 3.2e-174
Identity = 300/454 (66.08%), Postives = 362/454 (79.74%), Query Frame = 0


Query: 8   GNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNR 67
           GNTPL  VD EIF+L+ +EK RQ +G+ELIASENFTS AV+EALGS  TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 68  YYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGL 127
           YYGG E +DE+E LC+ RAL+AF +D +KWGVNVQPYSGSPANFA YTALL+PHDRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 128 DLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPKLIIC 187
           DLPSGGHLTHGY T   KKISA+SI+FES+PY++  A G IDY +LEE AL F+PKLII 
Sbjct: 126 DLPSGGHLTHGYQTD-KKKISASSIFFESMPYQI-GADGLIDYQRLEENALLFKPKLIIS 185

Query: 188 GGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLR 247
           G SAYPR+WDY R R IADK GA L+CDMAH SGLVAAQ   +PF+YCDVVT+TTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 248 GPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAM 307
           GPR+G+IF+R+G +    G+ E   YD E KINF+VFP+LQGGPH + I  +AVALK+A 
Sbjct: 246 GPRSGIIFFRRGKRVDGNGK-EIEEYDIESKINFAVFPSLQGGPHENVIAGVAVALKEAD 305

Query: 308 SPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLC 367
           S  FK YA QVK NA A+GN LMNKGYKLVT GT+NHL+LWDLRP  LTGNK EK  D+ 
Sbjct: 306 SQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEKAADIA 365

Query: 368 NITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEHG 427
           NITVNKNAV GD++A++PGG+RIG+ A+TSRGL E DFE+IA+FL R V+I+L +Q   G
Sbjct: 366 NITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEIQGRVG 425

Query: 428 KLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPG 462
           K L DF   +  +KE+  L+ +VE+FS  F +PG
Sbjct: 426 KKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

[Close]

BLAST of Carg10149 vs. ExPASy TrEMBL
Match: A0A6J1H4Y7 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE=3 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 8.4e-279
Identity = 469/471 (99.58%), Postives = 469/471 (99.58%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. ExPASy TrEMBL
Match: A0A6J1KX34 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499000 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.6e-277
Identity = 466/471 (98.94%), Postives = 469/471 (99.58%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGS+LTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSSLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. ExPASy TrEMBL
Match: A0A0A0LKC8 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.5e-272
Identity = 457/471 (97.03%), Postives = 464/471 (98.51%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           NVQKE+GKLLKDFNKGLVNNKEIE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. ExPASy TrEMBL
Match: A0A6J1CDF6 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.6e-272
Identity = 456/471 (96.82%), Postives = 463/471 (98.30%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           N+QKE+GKLLKDFNKGLVNNK+IE LKADVEKFSGSF+MPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. ExPASy TrEMBL
Match: A0A5A7SVK0 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold275G00690 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 6.4e-271
Identity = 454/471 (96.39%), Postives = 461/471 (97.88%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AF  DP KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFRCDPTKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFR IADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           N+QKE+GKLLKDFNKGLVNNKEIE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 902.5 bits (2331), Expect = 1.4e-262
Identity = 432/471 (91.72%), Postives = 455/471 (96.60%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV++WGNT LV+VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+  TG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFR IADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFKVYAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 472
           ++QK +GKLLKDFNKGLVNNK+++ LKADVEKFS S+EMPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

[Close]

BLAST of Carg10149 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 812.4 bits (2097), Expect = 1.9e-235
Identity = 381/468 (81.41%), Postives = 429/468 (91.67%), Query Frame = 0


Query: 1   MDPVNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L  VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TG+IDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFR +ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FKVYAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMK 469
           ++Q+++GK++KDFNKGLVNNKEI+ +KADVE+F+  F+MPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

[Close]

BLAST of Carg10149 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 629.4 bits (1622), Expect = 2.3e-180
Identity = 294/466 (63.09%), Postives = 369/466 (79.18%), Query Frame = 0


Query: 4   VNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WG+ P+   DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TG+IDYDKLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R+IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G        +  YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +K Y +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
           T  +Q+EHGK  K+F K L  NK+I  L+  VE F+  +EMP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

[Close]

BLAST of Carg10149 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 628.6 bits (1620), Expect = 4.0e-180
Identity = 297/466 (63.73%), Postives = 368/466 (78.97%), Query Frame = 0


Query: 4   VNTWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TG+IDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPENAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         N  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K+Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLM 465
               Q+EHGKL K+  K + + KEI  L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

[Close]

BLAST of Carg10149 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 565.1 bits (1455), Expect = 5.4e-161
Identity = 279/456 (61.18%), Postives = 344/456 (75.44%), Query Frame = 0


Query: 12  LVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGG 71
           L  VDPE+  +I KEK RQ R +ELIASENFTS AV+EA+GS LTNKYSEG+PG RYYGG
Sbjct: 83  LGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 142

Query: 72  NEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPS 131
           NE+ID++E LC++RAL AF +D  KWGVNVQP SGSPANFA YTA+L PHDRIMGLDLP 
Sbjct: 143 NEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPH 202

Query: 132 GGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDFRPKLIICGGSA 191
           GGHL+HG+ T+  +++S TSIYFES+PY++D +TG +DYD LE+ A  FRPKLII G SA
Sbjct: 203 GGHLSHGFMTA-KRRVSGTSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASA 262

Query: 192 YPRDWDYARFREIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRGPRA 251
           Y RD+DY R R+IAD  GA L+ DMAHISGLVAA   A+PFEYCD+VTTTTHKSLRGPR 
Sbjct: 263 YSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRG 322

Query: 252 GMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGF 311
           GMIF+RK         P N V D E  +N +VFP LQGGPHNH IG LAV LK A SP F
Sbjct: 323 GMIFFRK--------DPINGV-DLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEF 382

Query: 312 KVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITV 371
           K Y K+V +N  AL N L+  G+KLV+GG++NHLVL DLRP+G+ G +VEK+ D+ +IT+
Sbjct: 383 KAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITL 442

Query: 372 NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQK-EHGKLL 431
           NKN+V GD SAL PGG+RIG+PAMT+RGL EKDF  +A+F+   V IT+  +K   G  L
Sbjct: 443 NKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSKL 502

Query: 432 KDFNKGLVN-----NKEIEALKADVEKFSGSFEMPG 462
           +DFNK + +      + +++LK  VE F+  F +PG
Sbjct: 503 QDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:

BLAST of Carg10149 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013425.1	9.2e-280	100.00	Serine hydroxymethyltransferase 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022959093.1	1.7e-278	99.58	serine hydroxymethyltransferase 4-like [Cucurbita moschata] >KAG6574849.1 Serine...	[more]
XP_023548526.1	6.6e-278	99.36	serine hydroxymethyltransferase 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_023006191.1	3.3e-277	98.94	serine hydroxymethyltransferase 4-like [Cucurbita maxima]	[more]
XP_004138967.1	3.2e-272	97.03	serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical pro...	[more]

BLAST of Carg10149 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23254	2.0e-261	91.72	Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S...	[more]
Q9SVM4	2.7e-234	81.41	Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S...	[more]
Q84WV0	3.3e-179	63.09	Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S...	[more]
Q9LM59	5.6e-179	63.73	Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S...	[more]
Q54Z26	3.2e-174	66.08	Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ...	[more]

BLAST of Carg10149 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H4Y7	8.4e-279	99.58	Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE...	[more]
A0A6J1KX34	1.6e-277	98.94	Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499000 PE=3...	[more]
A0A0A0LKC8	1.5e-272	97.03	Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 ...	[more]
A0A6J1CDF6	2.6e-272	96.82	Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 P...	[more]
A0A5A7SVK0	6.4e-271	96.39	Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of Carg10149 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13930.1	1.4e-262	91.72	serine hydroxymethyltransferase 4	[more]
AT4G13890.1	1.9e-235	81.41	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT1G36370.1	2.3e-180	63.09	serine hydroxymethyltransferase 7	[more]
AT1G22020.1	4.0e-180	63.73	serine hydroxymethyltransferase 6	[more]
AT4G32520.1	5.4e-161	61.18	serine hydroxymethyltransferase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001085	Serine hydroxymethyltransferase	PIRSF	PIRSF000412	SHMT	coord: 1..466 e-value: 5.2E-189 score: 626.1
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 1..470
IPR001085	Serine hydroxymethyltransferase	HAMAP	MF_00051	SHMT	coord: 11..461 score: 42.914375
IPR001085	Serine hydroxymethyltransferase	CDD	cd00378	SHMT	coord: 15..453 e-value: 0.0 score: 618.833
IPR039429	Serine hydroxymethyltransferase-like domain	PFAM	PF00464	SHMT	coord: 13..411 e-value: 1.7E-192 score: 639.4
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 41..316 e-value: 2.6E-215 score: 716.8
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 16..460 e-value: 2.6E-215 score: 716.8
None	No IPR available	PANTHER	PTHR11680:SF33	SERINE HYDROXYMETHYLTRANSFERASE	coord: 1..470
IPR019798	Serine hydroxymethyltransferase, pyridoxal phosphate binding site	PROSITE	PS00096	SHMT	coord: 236..252
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 5..463

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10149-RA	Carg10149-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046655	folic acid metabolic process
biological_process	GO:0019264	glycine biosynthetic process from serine
biological_process	GO:0006565	L-serine catabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004372	glycine hydroxymethyltransferase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0070905	serine binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003824	catalytic activity