Carg10124 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10124
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncarboxyl-terminal-processing peptidase 2, chloroplastic
LocationCarg_Chr17: 673640 .. 675627 (+)
RNA-Seq ExpressionCarg10124
SyntenyCarg10124
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAATTATGTCCTTTGCAGGTGCTGTGAAAGAAGCGATTGATTATTTAAGGAGTAACAAAGTTAATGCCTTTGTACTTGACCTTCGGGATAATAGGTGATTAGTTTTCACTTTTCAGCACTTAAAGTTTTCCTTTTAGCACACCTCTTGAACTGTGGAACTTGTTGCAGTGGTGGTCTTTTTCCAGAAGGAGTTGAGATTGCCAAGATTTGGTACGTGCTATTTGGGTATATTATTTGGTGTGTTATTCATGCTGTTAGTTTCATTAATGGTGTAATATGACACAATGGATAATCCAAGTATATTACATCTGTAAAATTATCACTTTGCATTTAGGGAAAGGAGGTCTCTTGTAGCAATAAAAAGGCTAGACAAGTTTGGAACTTGATGTCGTAAAGAGATCGTCTCAAATGGAAATCAAATTCTATGGGAGGTAGGAAAGTTGCACTTAGAGTTAAAACTGAATCAACCAACGCCTCTTTTTGAAGCGTGTGATCAAAGAAGCAAAGGAATAAGATTCAAAGGGAATAATCAATCCCAAATTTTCTCCAAAAATGAATGCGAGATCCATTTCCTGTTGAAAACTAAAGAACTTGCTTTGTTCAGCAATGGGAAATGGATTCCCATATGAGATCCCACATCGGTTAGAGAAGGGAACGAAACATTCTTTATAAGGGTGTAGAAACCTCTTCCTAGTAGACACATTTTAAATGTTCTTTTGAGGGGAAGCCTAGAAGGGAGTTACATCCCAGGGCTCTTAGATGTGCTTTTTAGAAACCACATGTTCTATCGGCTGACAAACATATTTATCATGATAACCTTGTGGGACAAAGAATCTGACTATTGAAGGGAAAGTTCCATTACCACTTGGCTAGGTCAGGTAGTTTCCTATATGTTTGATTCCTTGTTCCTGCCCTCGTTAAACCCCTAGTAGAATGGTCTGTCTTTTTAAGTTTGTTTGGATATAGTTTAATGAATCAGTAGACTAATATTATTAGGTGACATTGATGTTGTGCATGTGTGAGATCTATCATTTCCATCTTTTCTCTTTTCCATTGATCATTTCATTTGCGATTTGGTCGGATCTCAATAATTTATCATACTTTTAACATAGTACTTGACATACAAGGATTCAAAAGTTGACAGTGAATCATTGTAGCTAGTAGAATGTCCTCTTAACGCTCTGCAGCCTGGTTTCATATTATCTGTTCTTATTCAGGTTGGACAAAGGTGTTATCGTGTATATTTGCGACAGTCGTGGTGTCCGAGATATATATGATTCAGATGGAAGTAGCTCTATAGCAGCTTCAGAACCATTAGCTGTGTTGGTAAACTTTCAGCTCCATTCTTTCTCTAAATAAAGGCTCCACCAAGTATCTCATCTATTCTTACTATTTACTTCATCATCACGACCTCCTTTTCTATGGATTCCTCTAAAGACTGCTTTAATATTTCTGTTACAATCACGATTATCCTCTTATTCTGCATTCTTTAAACTTTATTTAAACTTTACGAAAAATGTAAGCCTTTTAAACTTTATTTTGATCGTTTGAACATAACGCTTATATTAGGTGAACAAGGGAACTGCTAGTGCGAGTGAAATACTAGCCGGGGCATTGAAGGACAACAAGCGTGCTATATTATTTGGAGAACCAACCTATGGGAAAGGGTAAGAACTTTTGGCTGCGCCCCAAAACATGATTAGATGTGGTATTATCCAATTTCCTTACAAGAATTCACATCCTTCTCTGCAGCAAGATTCAGTCAGTCTTTAAACTATCAGACGGTTCTGGCTTGGCTGTGACAGTGGCTCGTTACGAGACACCAGCTCACATCGACATCGACAAGGTCTCTCTATCTCTTATCCCTACGAACACTTCTCAGGAAGACAACGTTCTTAACACGCCTGGATATTCTGCAGGTAGGAGTGATTCCAGACCGTCCGCTACCAGCATCATTCCCAAAGGATGGAGATGGTTTCTGTAG

mRNA sequence

ATGAGAATTATGTCCTTTGCAGGTGCTGTGAAAGAAGCGATTGATTATTTAAGGAGTAACAAAGTTAATGCCTTTGTACTTGACCTTCGGGATAATAGTGGTGGTCTTTTTCCAGAAGGAGTTGAGATTGCCAAGATTTGGTTGGACAAAGGTGTTATCGTGTATATTTGCGACAGTCGTGGTGTCCGAGATATATATGATTCAGATGGAAGTAGCTCTATAGCAGCTTCAGAACCATTAGCTGTGTTGGTGAACAAGGGAACTGCTAGTGCGAGTGAAATACTAGCCGGGGCATTGAAGGACAACAAGCGTGCTATATTATTTGGAGAACCAACCTATGGGAAAGGAATTCACATCCTTCTCTGCAGCAAGATTCAGTCAGTCTTTAAACTATCAGACGGTTCTGGCTTGGCTGTGACAGTGGCTCGTTACGAGACACCAGCTCACATCGACATCGACAAGGTCTCTCTATCTCTTATCCCTACGAACACTTCTCAGGAAGACAACGTTCTTAACACGCCTGGATATTCTGCAGGTAGGAGTGATTCCAGACCGTCCGCTACCAGCATCATTCCCAAAGGATGGAGATGGTTTCTGTAG

Coding sequence (CDS)

ATGAGAATTATGTCCTTTGCAGGTGCTGTGAAAGAAGCGATTGATTATTTAAGGAGTAACAAAGTTAATGCCTTTGTACTTGACCTTCGGGATAATAGTGGTGGTCTTTTTCCAGAAGGAGTTGAGATTGCCAAGATTTGGTTGGACAAAGGTGTTATCGTGTATATTTGCGACAGTCGTGGTGTCCGAGATATATATGATTCAGATGGAAGTAGCTCTATAGCAGCTTCAGAACCATTAGCTGTGTTGGTGAACAAGGGAACTGCTAGTGCGAGTGAAATACTAGCCGGGGCATTGAAGGACAACAAGCGTGCTATATTATTTGGAGAACCAACCTATGGGAAAGGAATTCACATCCTTCTCTGCAGCAAGATTCAGTCAGTCTTTAAACTATCAGACGGTTCTGGCTTGGCTGTGACAGTGGCTCGTTACGAGACACCAGCTCACATCGACATCGACAAGGTCTCTCTATCTCTTATCCCTACGAACACTTCTCAGGAAGACAACGTTCTTAACACGCCTGGATATTCTGCAGGTAGGAGTGATTCCAGACCGTCCGCTACCAGCATCATTCCCAAAGGATGGAGATGGTTTCTGTAG

Protein sequence

MRIMSFAGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIYDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQSVFKLSDGSGLAVTVARYETPAHIDIDKVSLSLIPTNTSQEDNVLNTPGYSAGRSDSRPSATSIIPKGWRWFL
Homology
BLAST of Carg10124 vs. NCBI nr
Match: KAG7013450.1 (Carboxyl-terminal-processing peptidase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.0 bits (1011), Expect = 7.4e-106
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MRIMSFAGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSR 60
           MRIMSFAGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSR
Sbjct: 1   MRIMSFAGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSR 60

Query: 61  GVRDIYDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHIL 120
           GVRDIYDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHIL
Sbjct: 61  GVRDIYDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHIL 120

Query: 121 LCSKIQSVFKLSDGSGLAVTVARYETPAHIDIDKVSLSLIPTNTSQEDNVLNTPGYSAGR 180
           LCSKIQSVFKLSDGSGLAVTVARYETPAHIDIDKVSLSLIPTNTSQEDNVLNTPGYSAGR
Sbjct: 121 LCSKIQSVFKLSDGSGLAVTVARYETPAHIDIDKVSLSLIPTNTSQEDNVLNTPGYSAGR 180

Query: 181 SDSRPSATSIIPKGWRWFL 200
           SDSRPSATSIIPKGWRWFL
Sbjct: 181 SDSRPSATSIIPKGWRWFL 199

BLAST of Carg10124 vs. NCBI nr
Match: XP_023006378.1 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_023006379.1 carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 271.9 bits (694), Expect = 4.3e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 357 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 416

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 417 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 476

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 477 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 500

BLAST of Carg10124 vs. NCBI nr
Match: XP_023548681.1 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271.9 bits (694), Expect = 4.3e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 355 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 414

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 415 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 474

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 475 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 498

BLAST of Carg10124 vs. NCBI nr
Match: XP_022959503.1 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 271.9 bits (694), Expect = 4.3e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 357 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 416

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 417 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 476

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 477 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 500

BLAST of Carg10124 vs. NCBI nr
Match: XP_023548679.1 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271.9 bits (694), Expect = 4.3e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 357 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 416

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 417 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 476

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 477 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 500

BLAST of Carg10124 vs. ExPASy Swiss-Prot
Match: O23614 (Carboxyl-terminal-processing peptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CTPA2 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-63
Identity = 123/152 (80.92%), Postives = 136/152 (89.47%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           + AV+EAI+ LR N VNAFVLDLRDNSGG FPEG+EIAK WLDKGVIVYICDSRGVRDIY
Sbjct: 334 SSAVREAIETLRGNNVNAFVLDLRDNSGGSFPEGIEIAKFWLDKGVIVYICDSRGVRDIY 393

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           D+DGS++IA SEPLAVLVNKGTASASEILAGALKDNKRA+++GEPTYGKG       KIQ
Sbjct: 394 DTDGSNAIATSEPLAVLVNKGTASASEILAGALKDNKRALVYGEPTYGKG-------KIQ 453

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSLS 159
           SVF+LSDGSGLAVTVARYETPAH DIDKV ++
Sbjct: 454 SVFELSDGSGLAVTVARYETPAHTDIDKVGVT 478

BLAST of Carg10124 vs. ExPASy Swiss-Prot
Match: O04073 (C-terminal processing peptidase, chloroplastic OS=Tetradesmus obliquus OX=3088 GN=ctpA PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 6.2e-39
Identity = 83/150 (55.33%), Postives = 113/150 (75.33%), Query Frame = 0

Query: 9   AVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIYDS 68
           A ++A   L    V   VLD+R+N GGLFP GV +A++ +D+G +V I DS+G+RDIY +
Sbjct: 292 AAQQAFTELSKQGVAGLVLDIRNNGGGLFPAGVNVARMLVDRGDLVLIADSQGIRDIYSA 351

Query: 69  DGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQSV 128
           DG +SI ++ PL VLVN+GTASASE+LAGALKD+KR ++ GE T+GKG+       IQ+V
Sbjct: 352 DG-NSIDSATPLVVLVNRGTASASEVLAGALKDSKRGLIAGERTFGKGL-------IQTV 411

Query: 129 FKLSDGSGLAVTVARYETPAHIDIDKVSLS 159
             LSDGSG+AVTVARY+TPA +DI+K+ +S
Sbjct: 412 VDLSDGSGVAVTVARYQTPAGVDINKIGVS 433

BLAST of Carg10124 vs. ExPASy Swiss-Prot
Match: Q55669 (Carboxyl-terminal-processing protease OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=ctpA PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.1e-34
Identity = 79/161 (49.07%), Postives = 111/161 (68.94%), Query Frame = 0

Query: 1   MRIMSF-AGAVKE---AIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYI 60
           +R+  F A A KE   A+  L     + ++LDLR+N GGL   G++IA++WL +  IVY 
Sbjct: 222 IRLSQFSANAYKEVAHALHQLEEQGADGYILDLRNNPGGLLQAGIDIARLWLPESTIVYT 281

Query: 61  CDSRGVRDIYDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG 120
            + +G ++ + ++G +  A   PL VLVN+GTASASEILAGAL+DN+RA L GE T+GKG
Sbjct: 282 VNRQGTQESFTANGEA--ATDRPLVVLVNQGTASASEILAGALQDNQRATLVGEKTFGKG 341

Query: 121 IHILLCSKIQSVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           +       IQS+F+LSDG+G+AVTVA+YETP H DI K+ +
Sbjct: 342 L-------IQSLFELSDGAGIAVTVAKYETPQHHDIHKLGI 373

BLAST of Carg10124 vs. ExPASy Swiss-Prot
Match: P42784 (Carboxyl-terminal-processing protease OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=ctpA PE=3 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 3.9e-33
Identity = 77/161 (47.83%), Postives = 110/161 (68.32%), Query Frame = 0

Query: 1   MRIMSFAGAVK----EAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYI 60
           +R+  F+   K    ++++ L+    + +VLDLR+N GGL   G+EIA++WLD+  IVY 
Sbjct: 219 IRLNQFSANAKTEIIKSLNQLQKQGADRYVLDLRNNPGGLLQAGIEIARLWLDQETIVYT 278

Query: 61  CDSRGVRDIYDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG 120
            + +G+ + Y + G     A  PL VLVN+ TASASEILAGAL+DN RA+L GE T+GKG
Sbjct: 279 VNRQGIFESYSAVGQPLTDA--PLVVLVNQATASASEILAGALQDNGRAMLVGEKTFGKG 338

Query: 121 IHILLCSKIQSVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           +       IQS+F+L DG+G+AVTVA+YETP H DI+K+ +
Sbjct: 339 L-------IQSLFELPDGAGMAVTVAKYETPLHHDINKLGI 370

BLAST of Carg10124 vs. ExPASy Swiss-Prot
Match: F4J3G5 (Carboxyl-terminal-processing peptidase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CTPA3 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.5e-27
Identity = 68/153 (44.44%), Postives = 100/153 (65.36%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLD-KGVIVYICDSRGVRDI 66
           A  ++ A+  + +  V +++LDLR+N GGL   G+++A++WLD    +VY  D  GV   
Sbjct: 324 ASDMENAVHEMENQDVQSYILDLRNNPGGLVRAGLDVAQLWLDGDETLVYTIDREGVTSP 383

Query: 67  YDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKI 126
            +     ++   +PL VLVN+G+ASASEILAGAL DN RAIL G  T+GKG       KI
Sbjct: 384 INMINGHAV-THDPLVVLVNEGSASASEILAGALHDNGRAILVGNRTFGKG-------KI 443

Query: 127 QSVFKLSDGSGLAVTVARYETPAHIDIDKVSLS 159
           QS+ +L+DGS L VTVA+Y +P+  +ID+V ++
Sbjct: 444 QSITELNDGSALFVTVAKYLSPSLHEIDQVGIA 468

BLAST of Carg10124 vs. ExPASy TrEMBL
Match: A0A6J1L202 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499128 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.1e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 357 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 416

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 417 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 476

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 477 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 500

BLAST of Carg10124 vs. ExPASy TrEMBL
Match: A0A6J1KZY7 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499128 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.1e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 355 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 414

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 415 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 474

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 475 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 498

BLAST of Carg10124 vs. ExPASy TrEMBL
Match: A0A6J1H887 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460464 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.1e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 355 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 414

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 415 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 474

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 475 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 498

BLAST of Carg10124 vs. ExPASy TrEMBL
Match: A0A6J1H648 (carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460464 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.1e-69
Identity = 141/151 (93.38%), Postives = 143/151 (94.70%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 357 SGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 416

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG       KIQ
Sbjct: 417 DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKG-------KIQ 476

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 477 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 500

BLAST of Carg10124 vs. ExPASy TrEMBL
Match: A0A0A0LIE8 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G165670 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 9.6e-67
Identity = 136/151 (90.07%), Postives = 141/151 (93.38%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           +GAVKEAID LRSN VNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY
Sbjct: 359 SGAVKEAIDSLRSNSVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 418

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           DSDGS++IAASEPLAVLVNKGTASASEILAGALKDNKRA+LFGEPTYGKG       KIQ
Sbjct: 419 DSDGSNTIAASEPLAVLVNKGTASASEILAGALKDNKRAMLFGEPTYGKG-------KIQ 478

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSL 158
           SVFKLSDGSGLAVTVARYETPAHIDIDKV +
Sbjct: 479 SVFKLSDGSGLAVTVARYETPAHIDIDKVGV 502

BLAST of Carg10124 vs. TAIR 10
Match: AT4G17740.1 (Peptidase S41 family protein )

HSP 1 Score: 244.2 bits (622), Expect = 8.9e-65
Identity = 123/152 (80.92%), Postives = 136/152 (89.47%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           + AV+EAI+ LR N VNAFVLDLRDNSGG FPEG+EIAK WLDKGVIVYICDSRGVRDIY
Sbjct: 334 SSAVREAIETLRGNNVNAFVLDLRDNSGGSFPEGIEIAKFWLDKGVIVYICDSRGVRDIY 393

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           D+DGS++IA SEPLAVLVNKGTASASEILAGALKDNKRA+++GEPTYGKG       KIQ
Sbjct: 394 DTDGSNAIATSEPLAVLVNKGTASASEILAGALKDNKRALVYGEPTYGKG-------KIQ 453

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSLS 159
           SVF+LSDGSGLAVTVARYETPAH DIDKV ++
Sbjct: 454 SVFELSDGSGLAVTVARYETPAHTDIDKVGVT 478

BLAST of Carg10124 vs. TAIR 10
Match: AT4G17740.2 (Peptidase S41 family protein )

HSP 1 Score: 244.2 bits (622), Expect = 8.9e-65
Identity = 123/152 (80.92%), Postives = 136/152 (89.47%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRGVRDIY 66
           + AV+EAI+ LR N VNAFVLDLRDNSGG FPEG+EIAK WLDKGVIVYICDSRGVRDIY
Sbjct: 324 SSAVREAIETLRGNNVNAFVLDLRDNSGGSFPEGIEIAKFWLDKGVIVYICDSRGVRDIY 383

Query: 67  DSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQ 126
           D+DGS++IA SEPLAVLVNKGTASASEILAGALKDNKRA+++GEPTYGKG       KIQ
Sbjct: 384 DTDGSNAIATSEPLAVLVNKGTASASEILAGALKDNKRALVYGEPTYGKG-------KIQ 443

Query: 127 SVFKLSDGSGLAVTVARYETPAHIDIDKVSLS 159
           SVF+LSDGSGLAVTVARYETPAH DIDKV ++
Sbjct: 444 SVFELSDGSGLAVTVARYETPAHTDIDKVGVT 468

BLAST of Carg10124 vs. TAIR 10
Match: AT3G57680.1 (Peptidase S41 family protein )

HSP 1 Score: 122.5 bits (306), Expect = 3.9e-28
Identity = 68/153 (44.44%), Postives = 100/153 (65.36%), Query Frame = 0

Query: 7   AGAVKEAIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLD-KGVIVYICDSRGVRDI 66
           A  ++ A+  + +  V +++LDLR+N GGL   G+++A++WLD    +VY  D  GV   
Sbjct: 324 ASDMENAVHEMENQDVQSYILDLRNNPGGLVRAGLDVAQLWLDGDETLVYTIDREGVTSP 383

Query: 67  YDSDGSSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKI 126
            +     ++   +PL VLVN+G+ASASEILAGAL DN RAIL G  T+GKG       KI
Sbjct: 384 INMINGHAV-THDPLVVLVNEGSASASEILAGALHDNGRAILVGNRTFGKG-------KI 443

Query: 127 QSVFKLSDGSGLAVTVARYETPAHIDIDKVSLS 159
           QS+ +L+DGS L VTVA+Y +P+  +ID+V ++
Sbjct: 444 QSITELNDGSALFVTVAKYLSPSLHEIDQVGIA 468

BLAST of Carg10124 vs. TAIR 10
Match: AT5G46390.2 (Peptidase S41 family protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.4e-25
Identity = 68/143 (47.55%), Postives = 88/143 (61.54%), Query Frame = 0

Query: 13  AIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKGVIVYICDSRG--VRDIYDSDG 72
           A+  L     + FV+DLRDN GGL   G+E AK++LD+G  V     R    +    SD 
Sbjct: 326 AMKRLLDKGASYFVMDLRDNLGGLVQAGIETAKLFLDEGDTVIYTAGRDPEAQKTVVSDK 385

Query: 73  SSSIAASEPLAVLVNKGTASASEILAGALKDNKRAILFGEPTYGKGIHILLCSKIQSVFK 132
              I A  PL V+VN  TASASEI+A AL DN +A+L GE TYGKG+       IQSV++
Sbjct: 386 KPLITA--PLIVMVNNRTASASEIVASALHDNCKAVLVGERTYGKGL-------IQSVYE 445

Query: 133 LSDGSGLAVTVARYETPAHIDID 154
           L DGSG+ VT+ +Y TP H+DI+
Sbjct: 446 LRDGSGVVVTIGKYVTPNHMDIN 459

BLAST of Carg10124 vs. TAIR 10
Match: AT5G46390.1 (Peptidase S41 family protein )

HSP 1 Score: 42.4 bits (98), Expect = 5.1e-04
Identity = 20/44 (45.45%), Postives = 29/44 (65.91%), Query Frame = 0

Query: 13  AIDYLRSNKVNAFVLDLRDNSGGLFPEGVEIAKIWLDKG-VIVY 56
           A+  L     + FV+DLRDN GGL   G+E AK++LD+G  ++Y
Sbjct: 326 AMKRLLDKGASYFVMDLRDNLGGLVQAGIETAKLFLDEGDTVIY 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013450.17.4e-106100.00Carboxyl-terminal-processing peptidase 2, chloroplastic, partial [Cucurbita argy... [more]
XP_023006378.14.3e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 [Cucurbi... [more]
XP_023548681.14.3e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X2 [Cucurbi... [more]
XP_022959503.14.3e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 [Cucurbi... [more]
XP_023548679.14.3e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 [Cucurbi... [more]
Match NameE-valueIdentityDescription
O236141.2e-6380.92Carboxyl-terminal-processing peptidase 2, chloroplastic OS=Arabidopsis thaliana ... [more]
O040736.2e-3955.33C-terminal processing peptidase, chloroplastic OS=Tetradesmus obliquus OX=3088 G... [more]
Q556692.1e-3449.07Carboxyl-terminal-processing protease OS=Synechocystis sp. (strain PCC 6803 / Ka... [more]
P427843.9e-3347.83Carboxyl-terminal-processing protease OS=Synechococcus sp. (strain ATCC 27264 / ... [more]
F4J3G55.5e-2744.44Carboxyl-terminal-processing peptidase 3, chloroplastic OS=Arabidopsis thaliana ... [more]
Match NameE-valueIdentityDescription
A0A6J1L2022.1e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 OS=Cucur... [more]
A0A6J1KZY72.1e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X2 OS=Cucur... [more]
A0A6J1H8872.1e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X2 OS=Cucur... [more]
A0A6J1H6482.1e-6993.38carboxyl-terminal-processing peptidase 2, chloroplastic-like isoform X1 OS=Cucur... [more]
A0A0A0LIE89.6e-6790.07PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G165670 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G17740.18.9e-6580.92Peptidase S41 family protein [more]
AT4G17740.28.9e-6580.92Peptidase S41 family protein [more]
AT3G57680.13.9e-2844.44Peptidase S41 family protein [more]
AT5G46390.22.4e-2547.55Peptidase S41 family protein [more]
AT5G46390.15.1e-0445.45Peptidase S41 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005151Tail specific proteaseSMARTSM00245tsp_4coord: 2..163
e-value: 1.4E-36
score: 137.5
IPR005151Tail specific proteasePFAMPF03572Peptidase_S41coord: 2..154
e-value: 2.4E-36
score: 124.8
NoneNo IPR availableGENE3D3.90.226.10coord: 2..170
e-value: 2.5E-41
score: 143.1
NoneNo IPR availablePANTHERPTHR32060:SF7CARBOXYL-TERMINAL-PROCESSING PEPTIDASE 2, CHLOROPLASTICcoord: 7..158
NoneNo IPR availablePANTHERPTHR32060TAIL-SPECIFIC PROTEASEcoord: 7..158
IPR004447C-terminal-processing peptidase S41ACDDcd07560Peptidase_S41_CPPcoord: 2..155
e-value: 6.68428E-63
score: 192.242
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 6..155

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10124-RACarg10124-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008236 serine-type peptidase activity