Carg10065 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10065
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMagnesium transporter
LocationCarg_Chr17: 1027434 .. 1031262 (-)
RNA-Seq ExpressionCarg10065
SyntenyCarg10065
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGGAATTTTGGAAAGAATTATAAAATATATTTTTGTAGCCATTATTGTTGTGATTGGAACCCTAATTAAGGTGGAGATTGAAGGTAAAAAAAAAAAAAAAAAAAAAAAACATGGCGTAGATCTGCAGAGATCAAAATTCTGGGTGTCTCTTCCTCCTCCGCCTCGATGCTGTGGCCTCATTCTCCATGTCATTGCCCCCACCTTCATTCCATATGTAAAACCCAAACTCAACTTGATATGCCCTCTCGAGAATCCAACCTCATCGCCCTCCTCTTCGGTTTCTTCTCTCTGCAACTCGGTGGAGAAGAAACTCCCAAACCCTTCCTTTTTCCTTTCGTTTCTCCAATACCCCACCTCCTAATTTCTTCCCCATTCCTTAATTCCGTCGTTCTTCTTCATTTCCGGTGAAGGGTTTTTGAGGGCTGCTTGGATTTCTTTCTTTTTCTTTCCTTCTTTTCTGGGAATGCGATTGATTTGGGGATGATTAGTAATGGCTCGGGACGGGCATGTACTGCCGGTGGATCCACAGACGACTCTCACTGTCAAGAAGAAGAAGCAATCTTCTCGGAATTGGATTCTGCTGGATTGTACGGGCCAAGGCACCGTCCTCGATGCCGACAAGTATCAAATTATGCATCGGGTTCAAATTCATGCGCGCGATCTTCGGATTCTTGACCCTTTGCTTTCTTATCCCTCAACCATTTTGGGGCGTGAGAGGGCCATTGTTCTTAATCTCGAGGTGAATTGTTTGGTTTTTTCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACGAATTGTTCTTGTTCTTTCTTGTAATTCTTGTAATTCTTGTAATTCTTGTTTGCTTATGCGTATTGCAGCATATAAAGGCGATCATTACTGCTGAAGAGGTCTGTATTTATACTTTTGTTCTTCTTTAATCTGTTTGTAAATTTGTGTGATTGTTGGAAAGTTGAAACGAAATTAGTATCAGACACTCCCTTGAATTTGTTACTTCATGTCGTGGATAGGTTTTTCTTAGAGATCCGACAGATGAACATGTCATCCCTGTGGTTGAGGAGCTTCAGAGGCGGCTGCCTCCTTCTAATGCGGTCCAGCTTCATGTTCAAGGAGATGGGAAAGCAAATCAGGGTCGGCAACACGACGGTGAAGCTGAAGAAGATGGTAAGCTTTCTTCCTCACTCACTGCAATGTGATCTTAACTTGTCCTTGTGTGATTTGTTTTTACTTCATCAATATATTAATGATCTTGAGAGAATGCATTCCAGACTCGCCCTTTGAATTTCGTGCGCTCGAGGTAGCTTTGGAAGCTATATGCAGTTATCTTGCTGCACGAACAACTGAGCTCGAGACTGCTGCTTATCCTGCCTTGGATGAGCTTACTGCCAAGGTGAGTACTTATTTTCTCACCTCATATGTAATGCATAAACTTCCCAACAATTCTGTTAAGTTATCGACTCCATCTTGGGATACTAGCATGATTTATTATTGTTTTTCCTTCGAGTTTGTAGATTAGTAGCCGAAACTTGGACAGAGTTCGTAAATTGAAGAGTGCAATGACCAGGCTGACTGCGAGGGTTCAAAAGGTACGTGTAATGTTTGTAACGTTTGTAACGTTTCACATATCAACACAATTTAGAAACTTTTGCCCATTGTAATCACTCTCATCTCTCTGTATGTTCCTAAATTCTGAATTTCTTACTAAATGATCAGCAGCTGTTATCTGCATAACCATAGTTTTGACTTGCCTTTCAATGCAGGTGAGAGATGAGCTCGAACAATTACTGAATGATGATGATGATATGGCAGACCTTTACTTGTCAAGAAAGATGAATTCGTCTTCTCCTGTAAGTGGTTCTGGTCCTGCCATTTGGTTTCCTGCCTCTCCTACCATTGGTTCAAAGATATCAAGAGCAAGCCGAGCAAGTATTGCAACTGGACGTGGGGATGAGGATGACATCGAGGAGCTCGAAATGTTACTGGAGGTAACAATACATATAGTTTCTGGTTAGTCGATCTTCATGAAGGTCTATAACTGAGATTGCATTTTGCAGGCTTACTTTATGCAGATTGATGGTACATTGAACAAATTAACTACAGTAAGTTTTACCTGTTTCCTGCTTATCTAATTTATGTTCAAGCTGAGATATTACGAGTTCGATATTACTTCGCTATATTTACATTGCCGGGAACGGTTTCTTATTAACTTAGAGTAATTGGATGCTAAAACACAAAATTGGATCTTGTTGAACCTTGACCAACAATTTTATTTATCAACTAGCAGCTAAGTGGGGAAGATTTGGAAGCTTCCCTTTCTCTCCCAAGTTCATAAGAGAACCACAGTACAGAAATTTAGCAAAAATATACCTCCTATACTATATTCTCCGTGTCCCAACGTGTCCCATGCCTCTTTTGCCATGGTTGCGCTTGCTATCTTCTCAAAATTTGACTCATTAACCACTTGGAATAATAAGTACAACTCGGTTTTATCCTTCATCTATGCTTGTTTTTGCTTCTTGATGTCATTTACTGTCATGTCTTCGATTACCTCCGGTTCGATATAACTGACATCAACCATGTCCCATTCATTTTGCGCCCCAAGCAAAGCTCACATTTGAATGCTTTAGTTGTTGTAGTTCAACTTCACCAGACATGCCGGTGGAATACCACCCTTCGACATCTTCCTAGAACTCGCCATTTTTGGCTTTGATGCCATTTTGTTGGCGTAACATAGAATTTTTATTGGAAAAGGGTAGAGTTCTCTCAACACTCAAATCCGTCTTTGTTATTTTCTAGAGGAAGAGTTATAATCTTTTCTTAAGTTGATTTGAAATCTCTACGGGAGTGGTCCTTATATAGGTCTTTCGGAATATTCTCCATGGCCATACATGTACCAAATATTAAATAACGAATGTACATAATACATGTACTATTATTACGGTGCTACATGTATCTACCAAATGAATCTAACTCCAAAATGTATCCAATAAACTTTTTAGATTTGGTATTTTCAAAATCTCTTCATGTTTTATTTATTTAACGTTTTTACCCATAAAATATATGAAACAACATCTTCCTGGCTTAATTGTCTACTGAAATATTACGATATTTGGACTAAACCCGATGAAGAACGATGGCCGTTAATCTAAGGGCATATTTGCTTATGTTTTGAATGATTTGCAGCTGCGAGAATACATTGATGACACAGAAGATTATATCAATATCCAGGTAACAAGAAGTGAAAGGTTCCGATCCACTACTGATCATCTCTTTCTTCTTCTTCTTCTTCTTTGTTTCTGAGTTATCATTGGTTTTGCAGCTTGACAATCACCGAAATCAACTCATTCAGGTCCTACATCCTTGCATATATTAATTAATGAGATTTTCTTGGCTGCCCAGATTCTTATTTATTAAAATTTCAAGTTTGCTGGGTGTCTTGATCTGCAGTTGGAGCTCTTTTTAAGCTCTGGAACTGTTTGTTTGTCTGTCTATTCTTTGGTGGGTGCAATATTCGGCATGAACATTCCATACACTTGGAATGAAAATCACGAATACATGTTTAAATGGGTATGGCTGAAGAACTCGACTATGATTTCTAACTTCAAATGCGGGAACCCGAACAAGAGCTCATAACCATCATCACGTCTTCATTATGTGCAGGTTGTCATAGTTTCTGGAGTAGCTTGTGCGTTAATGTTCGTCGCAATTATTTACTACGCTCGCCTCAAAGGTTTGGTCGGATCTTGATCTTCTTGTATTCGAACCGATCCTTTACAAGATCATAAGGAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAA

mRNA sequence

TTTTGGAATTTTGGAAAGAATTATAAAATATATTTTTGTAGCCATTATTGTTGTGATTGTAAAAAAAAAAAAAAAAAAAAAAAACATGGCGTAGATCTGCAGAGATCAAAATTCTGGGTGTCTCTTCCTCCTCCGCCTCGATGCTGTGGCCTCATTCTCCATGTCATTGCCCCCACCTTCATTCCATATGTAAAACCCAAACTCAACTTGATATGCCCTCTCGAGAATCCAACCTCATCGCCCTCCTCTTCGGTTTCTTCTCTCTGCAACTCGGTGGAGAAGAAACTCCCAAACCCTTCCTTTTTCCTTTCGTTTCTCCAATACCCCACCTCCTAATTTCTTCCCCATTCCTTAATTCCGTCGTTCTTCTTCATTTCCGGTGAAGGGTTTTTGAGGGCTGCTTGGATTTCTTTCTTTTTCTTTCCTTCTTTTCTGGGAATGCGATTGATTTGGGGATGATTAGTAATGGCTCGGGACGGGCATGTACTGCCGGTGGATCCACAGACGACTCTCACTGTCAAGAAGAAGAAGCAATCTTCTCGGAATTGGATTCTGCTGGATTGTACGGGCCAAGGCACCGTCCTCGATGCCGACAAGTATCAAATTATGCATCGGGTTCAAATTCATGCGCGCGATCTTCGGATTCTTGACCCTTTGCTTTCTTATCCCTCAACCATTTTGGGGCGTGAGAGGGCCATTGTTCTTAATCTCGAGCATATAAAGGCGATCATTACTGCTGAAGAGGTTTTTCTTAGAGATCCGACAGATGAACATGTCATCCCTGTGGTTGAGGAGCTTCAGAGGCGGCTGCCTCCTTCTAATGCGGTCCAGCTTCATGTTCAAGGAGATGGGAAAGCAAATCAGGGTCGGCAACACGACGGTGAAGCTGAAGAAGATGACTCGCCCTTTGAATTTCGTGCGCTCGAGGTAGCTTTGGAAGCTATATGCAGTTATCTTGCTGCACGAACAACTGAGCTCGAGACTGCTGCTTATCCTGCCTTGGATGAGCTTACTGCCAAGATTAGTAGCCGAAACTTGGACAGAGTTCGTAAATTGAAGAGTGCAATGACCAGGCTGACTGCGAGGGTTCAAAAGGTGAGAGATGAGCTCGAACAATTACTGAATGATGATGATGATATGGCAGACCTTTACTTGTCAAGAAAGATGAATTCGTCTTCTCCTGTAAGTGGTTCTGGTCCTGCCATTTGGTTTCCTGCCTCTCCTACCATTGGTTCAAAGATATCAAGAGCAAGCCGAGCAAGTATTGCAACTGGACGTGGGGATGAGGATGACATCGAGGAGCTCGAAATGTTACTGGAGGCTTACTTTATGCAGATTGATGGTACATTGAACAAATTAACTACACTGCGAGAATACATTGATGACACAGAAGATTATATCAATATCCAGCTTGACAATCACCGAAATCAACTCATTCAGTTGGAGCTCTTTTTAAGCTCTGGAACTGTTTGTTTGTCTGTCTATTCTTTGGTGGGTGCAATATTCGGCATGAACATTCCATACACTTGGAATGAAAATCACGAATACATGTTTAAATGGGTTGTCATAGTTTCTGGAGTAGCTTGTGCGTTAATGTTCGTCGCAATTATTTACTACGCTCGCCTCAAAGGTTTGGTCGGATCTTGATCTTCTTGTATTCGAACCGATCCTTTACAAGATCATAAGGAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAA

Coding sequence (CDS)

ATGGCTCGGGACGGGCATGTACTGCCGGTGGATCCACAGACGACTCTCACTGTCAAGAAGAAGAAGCAATCTTCTCGGAATTGGATTCTGCTGGATTGTACGGGCCAAGGCACCGTCCTCGATGCCGACAAGTATCAAATTATGCATCGGGTTCAAATTCATGCGCGCGATCTTCGGATTCTTGACCCTTTGCTTTCTTATCCCTCAACCATTTTGGGGCGTGAGAGGGCCATTGTTCTTAATCTCGAGCATATAAAGGCGATCATTACTGCTGAAGAGGTTTTTCTTAGAGATCCGACAGATGAACATGTCATCCCTGTGGTTGAGGAGCTTCAGAGGCGGCTGCCTCCTTCTAATGCGGTCCAGCTTCATGTTCAAGGAGATGGGAAAGCAAATCAGGGTCGGCAACACGACGGTGAAGCTGAAGAAGATGACTCGCCCTTTGAATTTCGTGCGCTCGAGGTAGCTTTGGAAGCTATATGCAGTTATCTTGCTGCACGAACAACTGAGCTCGAGACTGCTGCTTATCCTGCCTTGGATGAGCTTACTGCCAAGATTAGTAGCCGAAACTTGGACAGAGTTCGTAAATTGAAGAGTGCAATGACCAGGCTGACTGCGAGGGTTCAAAAGGTGAGAGATGAGCTCGAACAATTACTGAATGATGATGATGATATGGCAGACCTTTACTTGTCAAGAAAGATGAATTCGTCTTCTCCTGTAAGTGGTTCTGGTCCTGCCATTTGGTTTCCTGCCTCTCCTACCATTGGTTCAAAGATATCAAGAGCAAGCCGAGCAAGTATTGCAACTGGACGTGGGGATGAGGATGACATCGAGGAGCTCGAAATGTTACTGGAGGCTTACTTTATGCAGATTGATGGTACATTGAACAAATTAACTACACTGCGAGAATACATTGATGACACAGAAGATTATATCAATATCCAGCTTGACAATCACCGAAATCAACTCATTCAGTTGGAGCTCTTTTTAAGCTCTGGAACTGTTTGTTTGTCTGTCTATTCTTTGGTGGGTGCAATATTCGGCATGAACATTCCATACACTTGGAATGAAAATCACGAATACATGTTTAAATGGGTTGTCATAGTTTCTGGAGTAGCTTGTGCGTTAATGTTCGTCGCAATTATTTACTACGCTCGCCTCAAAGGTTTGGTCGGATCTTGA

Protein sequence

MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRILDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNAVQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALDELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPVSGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHEYMFKWVVIVSGVACALMFVAIIYYARLKGLVGS
Homology
BLAST of Carg10065 vs. NCBI nr
Match: KAG7013508.1 (Magnesium transporter MRS2-I [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 763.8 bits (1971), Expect = 7.1e-217
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI
Sbjct: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
           VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD
Sbjct: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS
Sbjct: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 393

BLAST of Carg10065 vs. NCBI nr
Match: KAG6574943.1 (Magnesium transporter MRS2-I, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 763.8 bits (1971), Expect = 7.1e-217
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI
Sbjct: 13  MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 72

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA
Sbjct: 73  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 132

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
           VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD
Sbjct: 133 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 192

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV
Sbjct: 193 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 252

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 253 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 312

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE
Sbjct: 313 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 372

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS
Sbjct: 373 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 405

BLAST of Carg10065 vs. NCBI nr
Match: XP_022959053.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 755.4 bits (1949), Expect = 2.5e-214
Identity = 389/393 (98.98%), Postives = 391/393 (99.49%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
           VQLHVQGDGKANQG QHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD
Sbjct: 121 VQLHVQGDGKANQGPQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPAIWFPASPTIGSKISRASRAS+AT RGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPAIWFPASPTIGSKISRASRASMATVRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS
Sbjct: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 393

BLAST of Carg10065 vs. NCBI nr
Match: XP_023548743.1 (magnesium transporter MRS2-I-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755.0 bits (1948), Expect = 3.3e-214
Identity = 389/393 (98.98%), Postives = 391/393 (99.49%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDE VIPVVEELQRRLPPSNA
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEQVIPVVEELQRRLPPSNA 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
           VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD
Sbjct: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPAIWFPASPTIGSKISRASRAS+AT RGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPAIWFPASPTIGSKISRASRASMATVRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS
Sbjct: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 393

BLAST of Carg10065 vs. NCBI nr
Match: XP_023006732.1 (magnesium transporter MRS2-I-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 753.8 bits (1945), Expect = 7.3e-214
Identity = 387/393 (98.47%), Postives = 391/393 (99.49%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
           VQLHVQGDGKA QGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD
Sbjct: 121 VQLHVQGDGKAYQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPAIWFPASPTIGSKISRASRAS+AT RGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPAIWFPASPTIGSKISRASRASMATVRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS+YSLVGAIFGMNIPYTWNENHE
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVGAIFGMNIPYTWNENHE 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIVSGVACA+MFVAIIYYARLKGLVGS
Sbjct: 361 YMFKWVVIVSGVACAIMFVAIIYYARLKGLVGS 393

BLAST of Carg10065 vs. ExPASy Swiss-Prot
Match: Q9FLG2 (Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 4.2e-164
Identity = 305/395 (77.22%), Postives = 342/395 (86.58%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MA++G+++P DP   +TVKKK   + +W L+D TGQ   LD DKY+IMHRVQIHARDLRI
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKTPQA-SWALIDATGQSEPLDVDKYEIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDP LSYPSTILGRERAIVLNLEHIKAIIT+EEV LRDP+DE+VIPVVEEL+RRLP  NA
Sbjct: 61  LDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVGNA 120

Query: 121 VQLHVQGDGKANQGRQHDGE-AEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPAL 180
                QGDGK   G Q+DG+  +ED+SPFEFRALEVALEAICS+LAART ELETAAYPAL
Sbjct: 121 SHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAELETAAYPAL 180

Query: 181 DELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNS-SS 240
           DELT+KISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLL+DDDDMADLYLSRK++S SS
Sbjct: 181 DELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKLSSASS 240

Query: 241 PVSGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKL 300
           P+S  G   W+  SPTIGSKISRASRAS+AT  GDE+D+EELEMLLEAYFMQID TLN+L
Sbjct: 241 PISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLEAYFMQIDSTLNRL 300

Query: 301 TTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNEN 360
           TTLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  IFGMNIPYTWN+ 
Sbjct: 301 TTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMNIPYTWNDG 360

Query: 361 HEYMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           H YMFK+VV ++G  C ++FV I+ YAR KGLVGS
Sbjct: 361 HGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of Carg10065 vs. ExPASy Swiss-Prot
Match: B8AJT9 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.3e-160
Identity = 300/375 (80.00%), Postives = 328/375 (87.47%), Query Frame = 0

Query: 20  KKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRILDPLLSYPSTILGRERAIV 79
           KK+ +SR+WIL D  G+  VLDADKY IMHRV I+ARDLRILDPLLSYPSTILGRERAIV
Sbjct: 20  KKRGASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRILDPLLSYPSTILGRERAIV 79

Query: 80  LNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNAVQLHVQGDGKANQGRQHDG 139
           LNLEHIKAIITAEEV LRDP D++VIPVVEEL+RRL PS+A Q  V+G            
Sbjct: 80  LNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSATQHDVEG------------ 139

Query: 140 EAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALDELTAKISSRNLDRVRKLKS 199
            AEED+SPFEFRALEV LEAICS+L ARTTELE+AAYPALDELT+KISSRNLDRVRKLKS
Sbjct: 140 -AEEDESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELTSKISSRNLDRVRKLKS 199

Query: 200 AMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKM-NSSSPVSGSGPAIWFPASPTIGSK 259
            MTRL ARVQKVRDELEQLL+DDDDMADLYLSRK+  ++SPVSGSG   WFPASPTIGSK
Sbjct: 200 GMTRLNARVQKVRDELEQLLDDDDDMADLYLSRKLAGAASPVSGSGGPNWFPASPTIGSK 259

Query: 260 ISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDN 319
           ISRASRAS  T  G+E+D+EELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDN
Sbjct: 260 ISRASRASAPTIHGNENDVEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDN 319

Query: 320 HRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHEYMFKWVVIVSGVACALMF 379
           HRNQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTWN+NH Y+FKWVV+VSG+ CA MF
Sbjct: 320 HRNQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYVFKWVVLVSGLFCAFMF 379

Query: 380 VAIIYYARLKGLVGS 394
           V+I+ YAR KGLVGS
Sbjct: 380 VSIVAYARHKGLVGS 381

BLAST of Carg10065 vs. ExPASy Swiss-Prot
Match: Q10D38 (Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I PE=2 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.3e-160
Identity = 300/375 (80.00%), Postives = 328/375 (87.47%), Query Frame = 0

Query: 20  KKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRILDPLLSYPSTILGRERAIV 79
           KK+ +SR+WIL D  G+  VLDADKY IMHRV I+ARDLRILDPLLSYPSTILGRERAIV
Sbjct: 23  KKRGASRSWILFDAAGEERVLDADKYAIMHRVDINARDLRILDPLLSYPSTILGRERAIV 82

Query: 80  LNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNAVQLHVQGDGKANQGRQHDG 139
           LNLEHIKAIITAEEV LRDP D++VIPVVEEL+RRL PS+A Q  V+G            
Sbjct: 83  LNLEHIKAIITAEEVLLRDPLDDNVIPVVEELRRRLAPSSATQHDVEG------------ 142

Query: 140 EAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALDELTAKISSRNLDRVRKLKS 199
            AEED+SPFEFRALEV LEAICS+L ARTTELE+AAYPALDELT+KISSRNLDRVRKLKS
Sbjct: 143 -AEEDESPFEFRALEVTLEAICSFLGARTTELESAAYPALDELTSKISSRNLDRVRKLKS 202

Query: 200 AMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKM-NSSSPVSGSGPAIWFPASPTIGSK 259
            MTRL ARVQKVRDELEQLL+DDDDMADLYLSRK+  ++SPVSGSG   WFPASPTIGSK
Sbjct: 203 GMTRLNARVQKVRDELEQLLDDDDDMADLYLSRKLAGAASPVSGSGGPNWFPASPTIGSK 262

Query: 260 ISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDN 319
           ISRASRAS  T  G+E+D+EELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDN
Sbjct: 263 ISRASRASAPTIHGNENDVEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLDN 322

Query: 320 HRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHEYMFKWVVIVSGVACALMF 379
           HRNQLIQLELFLSSGTVCLS+YSLV  IFGMNIPYTWN+NH Y+FKWVV+VSG+ CA MF
Sbjct: 323 HRNQLIQLELFLSSGTVCLSLYSLVAGIFGMNIPYTWNDNHGYVFKWVVLVSGLFCAFMF 382

Query: 380 VAIIYYARLKGLVGS 394
           V+I+ YAR KGLVGS
Sbjct: 383 VSIVAYARHKGLVGS 384

BLAST of Carg10065 vs. ExPASy Swiss-Prot
Match: Q304A0 (Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.5e-150
Identity = 287/396 (72.47%), Postives = 322/396 (81.31%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKK-KQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLR 60
           M+ DG ++PVD    +T K+K  Q SR+WI +D TGQ TVLD DK+ IMHRVQIHARDLR
Sbjct: 1   MSPDGELVPVDSSAVVTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLR 60

Query: 61  ILDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSN 120
           ILDP L YPS ILGRERAIVLNLEHIKAIITAEEV +RD +DE+VIPV+EE QRRLP  N
Sbjct: 61  ILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGN 120

Query: 121 AVQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPAL 180
                V GDG            EED+SPFEFRALEVALEAICS+LAARTTELE  AYPAL
Sbjct: 121 EAH-GVHGDGDL---------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 180

Query: 181 DELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKM-NSSS 240
           DELT KISSRNL+RVRKLKSAMTRLTARVQKVRDELEQLL+DD DMADLYL+RK+  +SS
Sbjct: 181 DELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASS 240

Query: 241 PVSGSGPAIWFPASPTIGSKISRASRASIATGRG-DEDDIEELEMLLEAYFMQIDGTLNK 300
            VS S   IW+P SPTIGS ISRASR S+ T RG DE D+EELEMLLEAYFMQID TLNK
Sbjct: 241 SVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAYFMQIDSTLNK 300

Query: 301 LTTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNE 360
           LT LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+SVYS++  IFGMNIP TWN 
Sbjct: 301 LTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNH 360

Query: 361 NHEYMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           +H Y+FKWVV ++G  C ++FV I+ YAR +GL+GS
Sbjct: 361 DHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of Carg10065 vs. ExPASy Swiss-Prot
Match: P0CZ21 (Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 2.6e-121
Identity = 241/375 (64.27%), Postives = 295/375 (78.67%), Query Frame = 0

Query: 23  QSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRILDPLLSYPSTILGRERAIVLNL 82
           QSS +WI +D TG+ TVLD DKY IMHRVQIHARDLRILDP L YPS ILGRERAIVLNL
Sbjct: 16  QSSWSWISIDATGKKTVLDVDKYVIMHRVQIHARDLRILDPNLFYPSAILGRERAIVLNL 75

Query: 83  EHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNAVQLHVQGDGKANQGRQHDGEAE 142
           EHIKAIITA+EV ++D +DE++IP +EE Q RL   N          KA+ G+      E
Sbjct: 76  EHIKAIITAKEVLIQDSSDENLIPTLEEFQTRLSVGN----------KAHGGQLDGDVVE 135

Query: 143 EDDSPFEFRALEVALEAICSYLAARTTELETAAYPALDELTAKISSRNLDRVRKLKSAMT 202
           ED+S FEFRALEVALEAICS+LAART ELE +AYPALDELT K++SRNL RV KLKS+MT
Sbjct: 136 EDESAFEFRALEVALEAICSFLAARTIELEKSAYPALDELTLKLTSRNLLRVCKLKSSMT 195

Query: 203 RLTARVQKVRDELEQLLNDDDDMADLYLSRKM-NSSSPVSGSGPAI-WFPASPTIGSKIS 262
           RLTA+VQK++DELEQLL DD+DMA+LYLSRK+  +SSP   SG  I W+P SPTIG+KIS
Sbjct: 196 RLTAQVQKIKDELEQLLEDDEDMAELYLSRKLAGASSPAIDSGEHINWYPTSPTIGAKIS 255

Query: 263 RASRASI--ATGRGDE-DDIEELEMLLEAYFMQIDGTLNKLTTLREYIDDTEDYINIQLD 322
           RA    +  AT RGD+ +D+EE+EMLLEA+FMQID TLNKLT LREY+D+TED++NIQLD
Sbjct: 256 RAKSHLVRSATVRGDDKNDVEEVEMLLEAHFMQIDRTLNKLTELREYVDETEDFLNIQLD 315

Query: 323 NHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHEYMFKWVVIVSGVACALM 382
           + RNQLI+ E+ L++G++C+SVYS+V  I GMNIP+ WN   ++MFKWVV  +   CA++
Sbjct: 316 SSRNQLIKFEIILTAGSICVSVYSVVVGILGMNIPFPWNIK-KHMFKWVVSGTATVCAIL 375

Query: 383 FVAIIYYARLKGLVG 393
           FV I+ +AR K L G
Sbjct: 376 FVTIMSFARYKKLFG 379

BLAST of Carg10065 vs. ExPASy TrEMBL
Match: A0A6J1H4U5 (Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111460159 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.2e-214
Identity = 389/393 (98.98%), Postives = 391/393 (99.49%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
           VQLHVQGDGKANQG QHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD
Sbjct: 121 VQLHVQGDGKANQGPQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPAIWFPASPTIGSKISRASRAS+AT RGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPAIWFPASPTIGSKISRASRASMATVRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS
Sbjct: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 393

BLAST of Carg10065 vs. ExPASy TrEMBL
Match: A0A6J1KWM0 (Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111499377 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 3.5e-214
Identity = 387/393 (98.47%), Postives = 391/393 (99.49%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
           VQLHVQGDGKA QGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD
Sbjct: 121 VQLHVQGDGKAYQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPAIWFPASPTIGSKISRASRAS+AT RGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPAIWFPASPTIGSKISRASRASMATVRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS+YSLVGAIFGMNIPYTWNENHE
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVGAIFGMNIPYTWNENHE 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIVSGVACA+MFVAIIYYARLKGLVGS
Sbjct: 361 YMFKWVVIVSGVACAIMFVAIIYYARLKGLVGS 393

BLAST of Carg10065 vs. ExPASy TrEMBL
Match: A0A6J1CAT1 (Magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111009882 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.5e-201
Identity = 365/393 (92.88%), Postives = 379/393 (96.44%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQT LTVKKKKQSSRNWILLDCTGQGTVLD DKY IMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTALTVKKKKQSSRNWILLDCTGQGTVLDVDKYAIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIIT+EEV LRDPTDE+VIPVVEELQRRLPPSNA
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPTDENVIPVVEELQRRLPPSNA 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
            QL +QGDGK  QG QHDGEAEED+SPFEFRALEVALEAICS+LAARTTELETAAYPALD
Sbjct: 121 FQLQIQGDGKEYQGGQHDGEAEEDESPFEFRALEVALEAICSFLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLL+DDDDMADLYLSRKM SSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKMVSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPA WFPASPTIGSKISRASRASIAT RGDE+DIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPANWFPASPTIGSKISRASRASIATVRGDENDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS+YSLVGAIFGMNIPYTWN++H 
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVGAIFGMNIPYTWNDDHG 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           YMFKWVVIV+GVACA+MF+AIIYYAR KGLVGS
Sbjct: 361 YMFKWVVIVTGVACAVMFIAIIYYARYKGLVGS 393

BLAST of Carg10065 vs. ExPASy TrEMBL
Match: A0A0A0LNR4 (Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G199910 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.9e-196
Identity = 356/393 (90.59%), Postives = 371/393 (94.40%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQT LTVKKKKQSSRNWILLDCTGQGTVLD DK+ IMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTALTVKKKKQSSRNWILLDCTGQGTVLDVDKHAIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITA+EV LRDPTDEHVIPVVEELQRRLPPSN 
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITADEVLLRDPTDEHVIPVVEELQRRLPPSNT 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
            Q  VQGDGK  Q    DGEAEEDDSPFEFRALEVALEAICS+LAARTTELETAAYPALD
Sbjct: 121 FQFQVQGDGKEYQSGPQDGEAEEDDSPFEFRALEVALEAICSFLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLL+DDDDMADLYLSRKM+SSSPV
Sbjct: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKMSSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPA WFPASPTIGSKISRASRAS+AT RGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPANWFPASPTIGSKISRASRASVATVRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLS+YSLV AIFGMNIPYTWN+ H 
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSIYSLVTAIFGMNIPYTWNDGHG 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           ++FKWVVI+SG ACA++F+ IIYYAR KGLVGS
Sbjct: 361 FIFKWVVIISGFACAVLFITIIYYARYKGLVGS 393

BLAST of Carg10065 vs. ExPASy TrEMBL
Match: A0A6J1KK71 (Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111494758 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.2e-193
Identity = 355/393 (90.33%), Postives = 368/393 (93.64%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MARDGHV+PVDPQT LTVKKKKQSSR+WILLDCTGQGTVLD DKY IMHRVQIHARDLRI
Sbjct: 1   MARDGHVVPVDPQTALTVKKKKQSSRSWILLDCTGQGTVLDVDKYAIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEV LRDPTDEHVIPVVEELQRRLPPSN 
Sbjct: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVLLRDPTDEHVIPVVEELQRRLPPSNV 120

Query: 121 VQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPALD 180
            Q +        QG  HDGEAEED+SPFEFRALEVALEAICS+LAARTTELETAAYPALD
Sbjct: 121 FQEY--------QGGLHDGEAEEDESPFEFRALEVALEAICSFLAARTTELETAAYPALD 180

Query: 181 ELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNSSSPV 240
           ELT KISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLL+DDDDMADLYLSRK +SSSPV
Sbjct: 181 ELTTKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKTSSSSPV 240

Query: 241 SGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300
           SGSGPA WFPASPTIGSKISRASRASIAT RGDEDDIEELEMLLEAYFMQIDGTLNKLTT
Sbjct: 241 SGSGPANWFPASPTIGSKISRASRASIATARGDEDDIEELEMLLEAYFMQIDGTLNKLTT 300

Query: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNENHE 360
           LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVC+S+YSLV AIFGMNIPYTWN++H 
Sbjct: 301 LREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCMSIYSLVSAIFGMNIPYTWNDDHG 360

Query: 361 YMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           Y FKWVVIVSGVACAL+FV I+YYARLKGLVGS
Sbjct: 361 YTFKWVVIVSGVACALLFVVIVYYARLKGLVGS 385

BLAST of Carg10065 vs. TAIR 10
Match: AT5G64560.1 (magnesium transporter 9 )

HSP 1 Score: 578.9 bits (1491), Expect = 3.0e-165
Identity = 305/395 (77.22%), Postives = 342/395 (86.58%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MA++G+++P DP   +TVKKK   + +W L+D TGQ   LD DKY+IMHRVQIHARDLRI
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKTPQA-SWALIDATGQSEPLDVDKYEIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDP LSYPSTILGRERAIVLNLEHIKAIIT+EEV LRDP+DE+VIPVVEEL+RRLP  NA
Sbjct: 61  LDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVGNA 120

Query: 121 VQLHVQGDGKANQGRQHDGE-AEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPAL 180
                QGDGK   G Q+DG+  +ED+SPFEFRALEVALEAICS+LAART ELETAAYPAL
Sbjct: 121 SHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAELETAAYPAL 180

Query: 181 DELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNS-SS 240
           DELT+KISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLL+DDDDMADLYLSRK++S SS
Sbjct: 181 DELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKLSSASS 240

Query: 241 PVSGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKL 300
           P+S  G   W+  SPTIGSKISRASRAS+AT  GDE+D+EELEMLLEAYFMQID TLN+L
Sbjct: 241 PISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLEAYFMQIDSTLNRL 300

Query: 301 TTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNEN 360
           TTLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  IFGMNIPYTWN+ 
Sbjct: 301 TTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMNIPYTWNDG 360

Query: 361 HEYMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           H YMFK+VV ++G  C ++FV I+ YAR KGLVGS
Sbjct: 361 HGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVGS 394

BLAST of Carg10065 vs. TAIR 10
Match: AT5G64560.2 (magnesium transporter 9 )

HSP 1 Score: 552.4 bits (1422), Expect = 3.0e-157
Identity = 290/368 (78.80%), Postives = 322/368 (87.50%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKKKQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLRI 60
           MA++G+++P DP   +TVKKK   + +W L+D TGQ   LD DKY+IMHRVQIHARDLRI
Sbjct: 1   MAQNGYLVPADPSAVVTVKKKTPQA-SWALIDATGQSEPLDVDKYEIMHRVQIHARDLRI 60

Query: 61  LDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSNA 120
           LDP LSYPSTILGRERAIVLNLEHIKAIIT+EEV LRDP+DE+VIPVVEEL+RRLP  NA
Sbjct: 61  LDPNLSYPSTILGRERAIVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVGNA 120

Query: 121 VQLHVQGDGKANQGRQHDGE-AEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPAL 180
                QGDGK   G Q+DG+  +ED+SPFEFRALEVALEAICS+LAART ELETAAYPAL
Sbjct: 121 SHNGGQGDGKEIAGAQNDGDTGDEDESPFEFRALEVALEAICSFLAARTAELETAAYPAL 180

Query: 181 DELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKMNS-SS 240
           DELT+KISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLL+DDDDMADLYLSRK++S SS
Sbjct: 181 DELTSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKLSSASS 240

Query: 241 PVSGSGPAIWFPASPTIGSKISRASRASIATGRGDEDDIEELEMLLEAYFMQIDGTLNKL 300
           P+S  G   W+  SPTIGSKISRASRAS+AT  GDE+D+EELEMLLEAYFMQID TLN+L
Sbjct: 241 PISSIGEPNWYTTSPTIGSKISRASRASLATVHGDENDVEELEMLLEAYFMQIDSTLNRL 300

Query: 301 TTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNEN 360
           TTLREYIDDTEDYINIQLDNHRNQLIQLEL LSSGTVCLS+YSLV  IFGMNIPYTWN+ 
Sbjct: 301 TTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMNIPYTWNDG 360

Query: 361 HEYMFKWV 367
           H YMFK+V
Sbjct: 361 HGYMFKYV 367

BLAST of Carg10065 vs. TAIR 10
Match: AT5G09690.1 (magnesium transporter 7 )

HSP 1 Score: 532.3 bits (1370), Expect = 3.2e-151
Identity = 287/396 (72.47%), Postives = 322/396 (81.31%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKK-KQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLR 60
           M+ DG ++PVD    +T K+K  Q SR+WI +D TGQ TVLD DK+ IMHRVQIHARDLR
Sbjct: 1   MSPDGELVPVDSSAVVTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLR 60

Query: 61  ILDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSN 120
           ILDP L YPS ILGRERAIVLNLEHIKAIITAEEV +RD +DE+VIPV+EE QRRLP  N
Sbjct: 61  ILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGN 120

Query: 121 AVQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPAL 180
                V GDG            EED+SPFEFRALEVALEAICS+LAARTTELE  AYPAL
Sbjct: 121 EAH-GVHGDGDL---------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 180

Query: 181 DELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKM-NSSS 240
           DELT KISSRNL+RVRKLKSAMTRLTARVQKVRDELEQLL+DD DMADLYL+RK+  +SS
Sbjct: 181 DELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASS 240

Query: 241 PVSGSGPAIWFPASPTIGSKISRASRASIATGRG-DEDDIEELEMLLEAYFMQIDGTLNK 300
            VS S   IW+P SPTIGS ISRASR S+ T RG DE D+EELEMLLEAYFMQID TLNK
Sbjct: 241 SVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAYFMQIDSTLNK 300

Query: 301 LTTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNE 360
           LT LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+SVYS++  IFGMNIP TWN 
Sbjct: 301 LTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNH 360

Query: 361 NHEYMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           +H Y+FKWVV ++G  C ++FV I+ YAR +GL+GS
Sbjct: 361 DHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 386

BLAST of Carg10065 vs. TAIR 10
Match: AT5G09690.2 (magnesium transporter 7 )

HSP 1 Score: 532.3 bits (1370), Expect = 3.2e-151
Identity = 287/396 (72.47%), Postives = 322/396 (81.31%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKK-KQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLR 60
           M+ DG ++PVD    +T K+K  Q SR+WI +D TGQ TVLD DK+ IMHRVQIHARDLR
Sbjct: 12  MSPDGELVPVDSSAVVTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLR 71

Query: 61  ILDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSN 120
           ILDP L YPS ILGRERAIVLNLEHIKAIITAEEV +RD +DE+VIPV+EE QRRLP  N
Sbjct: 72  ILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGN 131

Query: 121 AVQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPAL 180
                V GDG            EED+SPFEFRALEVALEAICS+LAARTTELE  AYPAL
Sbjct: 132 EAH-GVHGDGDL---------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 191

Query: 181 DELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKM-NSSS 240
           DELT KISSRNL+RVRKLKSAMTRLTARVQKVRDELEQLL+DD DMADLYL+RK+  +SS
Sbjct: 192 DELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASS 251

Query: 241 PVSGSGPAIWFPASPTIGSKISRASRASIATGRG-DEDDIEELEMLLEAYFMQIDGTLNK 300
            VS S   IW+P SPTIGS ISRASR S+ T RG DE D+EELEMLLEAYFMQID TLNK
Sbjct: 252 SVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLEAYFMQIDSTLNK 311

Query: 301 LTTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNE 360
           LT LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+SVYS++  IFGMNIP TWN 
Sbjct: 312 LTELREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNH 371

Query: 361 NHEYMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           +H Y+FKWVV ++G  C ++FV I+ YAR +GL+GS
Sbjct: 372 DHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 397

BLAST of Carg10065 vs. TAIR 10
Match: AT5G09690.4 (magnesium transporter 7 )

HSP 1 Score: 496.5 bits (1277), Expect = 2.0e-140
Identity = 274/396 (69.19%), Postives = 309/396 (78.03%), Query Frame = 0

Query: 1   MARDGHVLPVDPQTTLTVKKK-KQSSRNWILLDCTGQGTVLDADKYQIMHRVQIHARDLR 60
           M+ DG ++PVD    +T K+K  Q SR+WI +D TGQ TVLD DK+ IMHRVQIHARDLR
Sbjct: 1   MSPDGELVPVDSSAVVTAKRKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLR 60

Query: 61  ILDPLLSYPSTILGRERAIVLNLEHIKAIITAEEVFLRDPTDEHVIPVVEELQRRLPPSN 120
           ILDP L YPS ILGRERAIVLNLEHIKAIITAEEV +RD +DE+VIPV+EE QRRLP  N
Sbjct: 61  ILDPNLFYPSAILGRERAIVLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGN 120

Query: 121 AVQLHVQGDGKANQGRQHDGEAEEDDSPFEFRALEVALEAICSYLAARTTELETAAYPAL 180
                V GDG            EED+SPFEFRALEVALEAICS+LAARTTELE  AYPAL
Sbjct: 121 EAH-GVHGDGDL---------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPAL 180

Query: 181 DELTAKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLNDDDDMADLYLSRKM-NSSS 240
           DELT KISSRNL+RVRKLKSAMTRLTARVQKVRDELEQLL+DD DMADLYL+RK+  +SS
Sbjct: 181 DELTLKISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASS 240

Query: 241 PVSGSGPAIWFPASPTIGSKISRASRASIATGRG-DEDDIEELEMLLEAYFMQIDGTLNK 300
            VS S   IW+P SPTIGS ISRASR S+ T RG DE D+EELEMLLE            
Sbjct: 241 SVSVSDEPIWYPTSPTIGSMISRASRVSLVTVRGDDETDVEELEMLLE------------ 300

Query: 301 LTTLREYIDDTEDYINIQLDNHRNQLIQLELFLSSGTVCLSVYSLVGAIFGMNIPYTWNE 360
              LREYIDDTEDYINIQLDNHRNQLIQLEL LS+GTVC+SVYS++  IFGMNIP TWN 
Sbjct: 301 ---LREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNH 360

Query: 361 NHEYMFKWVVIVSGVACALMFVAIIYYARLKGLVGS 394
           +H Y+FKWVV ++G  C ++FV I+ YAR +GL+GS
Sbjct: 361 DHGYIFKWVVSLTGTFCIVLFVIILSYARFRGLIGS 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013508.17.1e-217100.00Magnesium transporter MRS2-I [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6574943.17.1e-217100.00Magnesium transporter MRS2-I, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022959053.12.5e-21498.98magnesium transporter MRS2-I-like isoform X1 [Cucurbita moschata][more]
XP_023548743.13.3e-21498.98magnesium transporter MRS2-I-like [Cucurbita pepo subsp. pepo][more]
XP_023006732.17.3e-21498.47magnesium transporter MRS2-I-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FLG24.2e-16477.22Magnesium transporter MRS2-2 OS=Arabidopsis thaliana OX=3702 GN=MRS2-2 PE=2 SV=1[more]
B8AJT91.3e-16080.00Magnesium transporter MRS2-I OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-I PE... [more]
Q10D381.3e-16080.00Magnesium transporter MRS2-I OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-I ... [more]
Q304A04.5e-15072.47Magnesium transporter MRS2-7 OS=Arabidopsis thaliana OX=3702 GN=MRS2-7 PE=2 SV=1[more]
P0CZ212.6e-12164.27Magnesium transporter MRS2-8 OS=Arabidopsis thaliana OX=3702 GN=MRS2-8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H4U51.2e-21498.98Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111460159 PE=3 SV=1[more]
A0A6J1KWM03.5e-21498.47Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111499377 PE=3 SV=1[more]
A0A6J1CAT14.5e-20192.88Magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111009882 PE=3 SV=1[more]
A0A0A0LNR41.9e-19690.59Magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G199910 PE=3 SV=1[more]
A0A6J1KK711.2e-19390.33Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111494758 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64560.13.0e-16577.22magnesium transporter 9 [more]
AT5G64560.23.0e-15778.80magnesium transporter 9 [more]
AT5G09690.13.2e-15172.47magnesium transporter 7 [more]
AT5G09690.23.2e-15172.47magnesium transporter 7 [more]
AT5G09690.42.0e-14069.19magnesium transporter 7 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 194..221
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 104..323
e-value: 4.7E-69
score: 233.3
NoneNo IPR availableGENE3D2.40.128.330coord: 14..99
e-value: 1.4E-20
score: 74.5
NoneNo IPR availablePANTHERPTHR13890:SF43MAGNESIUM TRANSPORTER MRS2-Icoord: 1..393
NoneNo IPR availableSUPERFAMILY144083Magnesium transport protein CorA, transmembrane regioncoord: 329..381
IPR002523Mg2+ transporter protein, CorA-like/Zinc transport protein ZntBPFAMPF01544CorAcoord: 281..380
e-value: 8.2E-8
score: 32.0
IPR039204Magnesium transporter MRS2-likePANTHERPTHR13890RNA SPLICING PROTEIN MRS2, MITOCHONDRIALcoord: 1..393
IPR039204Magnesium transporter MRS2-likeCDDcd12823Mrs2_Mfm1p-likecoord: 28..390
e-value: 4.54197E-131
score: 377.355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10065-RACarg10065-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity