Carg09995 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09995
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Eukaryotic translation initiation factor 5A
Location	Carg_Chr10: 2154764 .. 2158543 (-)
RNA-Seq Expression	Carg09995
Synteny	Carg09995

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGACGAGGAGCACCACTTTGACTCTAGGGCCAACGCCAGAGAGCCCGAGACTTACCCTGAGGAAGCTAGAACCTGGCTACATCGTAATCAATAACAGACCCTGCAAGGTTTCCTTCTTTCCTTTTTTTCTCTGCATCTTTTCTGAGATTGACTTTGTTCTTCTTCCATGGAGTAAATTTTGGGGGCTTGTGCTATTCTTTGTTATAATTTTTCCTTGGCTCTTTGTAAGGGCAGACGTTTGATTGATTGTTGTTTCTGTACTTCTATTTCCTAGTGAAGATTGTCCCTGAATTTTTACTCCGTGACTACTGATTATGGCAATTTTTACTATTGTTTATGGTCTCCCCGATTGATTTAGGTTTCCCCTTTAATTTCTCTTGTTTCGCAACGTTGATGAAACCAGGGCCGTTTAGTTACGTATTATGTGTTATCCCACAGTAACAATTGCAGGGGGAAAATTTTCGAAGAGGTTGTGCAAAATTTGGCATAATCGGTTTATTGTTTTTAATGGCAAGTATTTCTTTCACCCAAATTGGATGCGTAATATGATTTGTCGCCTTGGTTTGCCAAAAATGCATATGGACTTGGCTTATTGACTTATTGAGTTTTGCTATGGGACATGCCGTCGAACAGTTCAACAGGAACATACGGGCCATATACTGTTTACCATATATCGTCAAATGTCCCCTGTTGTGTGTTGTACAGTGCAAACAATGAGGTAGATGTCAGGCTAAATGTTAATAGTGTTTAAAAACCACGAAATTGAAACTATCCACTTTGAGCATAAGCTCTCGTGACTCTATTTTTGGTTTTGGAGATATTATCCACTTTGAGCAATAGCTCTCATGACTCTATTTTTGGTTTTTCCAAAATGGCTTCTATCAATGGAGATGTATTCCTTACTTATAAATTCATAATCAACCTCTTAATTAGCTGATGTGAGACTCCTCTCCCGACAATCCTCAACAATCCTCCTATTGAACAAAGTACACCAGAGAGCCTTCCCTGAGACCTATGGAGCCCTTGAACAACCTCCCCTTAATAGAGGCTCGACTTCTTCTCTAGAGCCCTTGAACAAAGGCACCCTTTATTAAACTCTTGAGTCACTTTTGACTACTAAGAAGCAGTTTTCACAGCTCCTTATAGATTTGACGAGACTTTGAAACACTGAAATCAACTGAAACACATTGAAGAGACCTAAAAGTCAACTTCAGCCTACAACACAGAGTTGTAAAGAGTCGGTTGATAGAGATGTCAACCAGTTGAGTGGGGGAGGATATCACGGTCATGCTTGTCCAAGGCATGTTACCTATGGCCGCATGACAGATGTTCCAGTCTTGCTTGTCTAAGTCGGTTGACAGGGAGGATGTCATGGTCATGCTTGTCTTGCTTGTTCAAGTCAGTTGACAGGTCTTTGTTCAACTCTTGAGTCACTTTTGACTACATCTTCGAGGCTCACAACTTCTTTGTTTGACATTTGAGGATTCTGTTAGACATGACTAGATTAAGGGTATGGTTCTGATACCATGTTAAAAACCACAAACTTCCACAATAGTATGATATTGTCCACTTTGAGCATAAGCTTTCACGACTTTGTTTTTTGTTTTCCTAAAAGGAGTCCTACCAATGGAGATGTATTTCTTACCTATAAACCTATGATCAACTCCTTAATTAGCTGATGTGGGACTCCTCTCCCAACAATCCTCAACAAATAGTAGATATAATCTTCATGCTGCATTTGTCTTCTTGGTTAACAAAACGTCTTTGAAGCATTAGCAAAGCTTTTCTTTCCTAGAAAGAACTTTTGTATGCATGCCACCTTCTTTTTGTGTTGATACAATGTCCATGGTTATAGTTCCATTTTCCCTGGGACATTCCTTTGGAGCCTTCCTTGGCTCGAGCTATATGGAAAGATAAATACCCAACGAAGAATAGATTTTTTTCTTTGAGAAATCTCACACAAAGCTATCAACATCAAAGATCTCCTCAAAGATGTATGCCATGCCATTTCACCCGGTTGGTGCACTTTGATGATGAATTTCAAAACCACATTTTCATTGCGTATGACAATGATTTTCTGAAATGAACCATGGATATATGAAATAATATTCTTTCAAGCTACTTGGCCAAGCATATTTTGGCTGCCATATATCATCACTTTTTTCTATCTCCCTTCATTTCAGGTCGTTGAAGTTTCTACTTCAAAACCTGGAAAGCATGGACATGCAAAGTGCCACTTTGTGGCAATTGACATATTCAATGGGAAGAGACTTGAAGTATTTGTTCCTTCTTCTCAGAAATGCGATGTAATTTCTTTGTCACTCTCTTCTTGTGTTCTACTCTCTAAGTACTAATTTTATTCTACTAACAAACGTTGCTTGTATTCCTTGCAGGTTCCCTATGTTTATCACACCGACTATCATCTGATTGATATATGCGAGGATGGTTTTGTAAGTTTCAATTATATTGAAACATGATATTGCTTTATATACGAAGCTTAGATCAATTTTTATGTCTCTTGCTTCAATTTAGATTTGAGAAATATCCCCGTTCACCCCTTCCCAAGAGAGAGGGAGAGAAAGCTCCCTGTTATAACTTCATAACAAAAGTGGGAGACCCTGCATCAGTTGGAACATCAGTTAGAGAGGGGAACGAAGCATTCTTTATAAGGTTGGAGCATCAGTTAGAGAGGGGAACGAAGCATTATTTATAAGGTTGTGGAAATCTGACGCATTTTAAAAACTTTGAGGGGAAGCCTGAAGGAAAAGTCTAAAGAGGACAATATCTATTAGTGGTGGGCTTGGGCCGTTACAAATAGTATCAAAGCCAGTCACCGGATGATATGCTAGCAAGGACGTTGGCCCCCAAGGGGAGTGGATTGTGAGATCTCACATCGGTTGGAGAGGGGAATGAAGCATTCCTTATAAGGGTGTGGGAACCTCTCCCTAGCAGATGCGTTTTAAAAACTTTGAGGAGAAGCTCGAAACGGAAAGCCCAAAAAGGACCATATCGGCTAGTGGTGAGTTTGGGCCGTTAGTTCGATTGGAAGGAGTTTTGCAATCTTAATCTATAGCAGAGGATCTTGAGTCGAGACCTATTGACTTGGTAATTTTGCAGGTCAGTCTCCGTTCTGAAAATGGAAACATCAAGGATGGTCTGACGCTCCCCAAGGATGAAAATCTGCGTAGGCAGGTACATAAATGATTATACCATTACATTTACAAACCTTCAAGCCATTGCTTTATAGCTTTCTGCAGCACCATCTCTGTTCCATTTATTTTCATGCCAATAGCTTAAAGAGGGGTTCGCTCATAGAAAAGATCTTGTAGTAACTGTCATGTCTGCAATGGGAGAGGAGCAGATCTGTGCTCTCAAGGACATCGGTCCTAGAAGTTAAAACACAAAGCTATGGTTACACCTGATGGCTCAGAATCACCCCAACCAAAAGTTCTATCAAGTATGTTTATATGTCATAAAACCATATTCTCAGCAAGGTACTTCAAGCTATTGATCCTTGCACACTGCTGAAACGATACATACATGTTTTACTTTATATAGTACACTACTTATAAAATATGTAACCCCCCCTGTTTTCTAACAAGCCCTTTGAATAAGATCTTGGGTTCTAGTAGGAAGATAAACAAACAGGTTTTCTGGTCTTTTGGTCTCTTTCTTCTTTTGTCTAGAGGTTGTTATTAAGGTCATTGAAGCTCATCACTATCGAGTGGCTGTTGTTTCCTTGAAAAAGGGCGAGGGCTTGGCCCGACCGATATGA

mRNA sequence

ATGTCGGACGAGGAGCACCACTTTGACTCTAGGGCCAACGCCAGAGAGCCCGAGACTTACCCTGAGGAAGCTAGAACCTGGCTACATCAAATCTCACACAAAGCTATCAACATCAAAGATCTCCTCAAAGATGTCGTTGAAGTTTCTACTTCAAAACCTGGAAAGCATGGACATGCAAAGTGCCACTTTGTGGCAATTGACATATTCAATGGGAAGAGACTTGAAGTATTTGTTCCTTCTTCTCAGAAATGCGATGTTCCCTATGTTTATCACACCGACTATCATCTGATTGATATATGCGAGGATGGTTTTGATGGTCTGACGCTCCCCAAGGATGAAAATCTGCGTAGGCAGCTTAAAGAGGGGTTCGCTCATAGAAAAGATCTTGTAGTAACTGTCATGTCTGCAATGGGAGAGGAGCAGATCTGTGCTCTCAAGGACATCGGTCCTAGAAAGGTTGTTATTAAGGTCATTGAAGCTCATCACTATCGAGTGGCTGTTGTTTCCTTGAAAAAGGGCGAGGGCTTGGCCCGACCGATATGA

Coding sequence (CDS)

ATGTCGGACGAGGAGCACCACTTTGACTCTAGGGCCAACGCCAGAGAGCCCGAGACTTACCCTGAGGAAGCTAGAACCTGGCTACATCAAATCTCACACAAAGCTATCAACATCAAAGATCTCCTCAAAGATGTCGTTGAAGTTTCTACTTCAAAACCTGGAAAGCATGGACATGCAAAGTGCCACTTTGTGGCAATTGACATATTCAATGGGAAGAGACTTGAAGTATTTGTTCCTTCTTCTCAGAAATGCGATGTTCCCTATGTTTATCACACCGACTATCATCTGATTGATATATGCGAGGATGGTTTTGATGGTCTGACGCTCCCCAAGGATGAAAATCTGCGTAGGCAGCTTAAAGAGGGGTTCGCTCATAGAAAAGATCTTGTAGTAACTGTCATGTCTGCAATGGGAGAGGAGCAGATCTGTGCTCTCAAGGACATCGGTCCTAGAAAGGTTGTTATTAAGGTCATTGAAGCTCATCACTATCGAGTGGCTGTTGTTTCCTTGAAAAAGGGCGAGGGCTTGGCCCGACCGATATGA

Protein sequence

MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAKCHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGFDGLTLPKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPRKVVIKVIEAHHYRVAVVSLKKGEGLARPI

Homology

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BLAST of Carg09995 vs. NCBI nr
Match: KAG7023445.1 (Eukaryotic translation initiation factor 5A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374.0 bits (959), Expect = 7.2e-100
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK
Sbjct: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGFDGLTLPKDENLRRQLK 120
           CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGFDGLTLPKDENLRRQLK
Sbjct: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGFDGLTLPKDENLRRQLK 120

Query: 121 EGFAHRKDLVVTVMSAMGEEQICALKDIGPRKVVIKVIEAHHYRVAVVSLKKGEGLARPI 180
           EGFAHRKDLVVTVMSAMGEEQICALKDIGPRKVVIKVIEAHHYRVAVVSLKKGEGLARPI
Sbjct: 121 EGFAHRKDLVVTVMSAMGEEQICALKDIGPRKVVIKVIEAHHYRVAVVSLKKGEGLARPI 180

[Close]

BLAST of Carg09995 vs. NCBI nr
Match: KAG6589773.1 (hypothetical protein SDJN03_15196, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 258.8 bits (660), Expect = 3.4e-65
Identity = 132/162 (81.48%), Postives = 137/162 (84.57%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHFDS+ANAREPETYPEEART + +  +  IN +     VVEVSTSKPGKHGHAK
Sbjct: 1   MSDEEHHFDSKANAREPETYPEEART-IRKNGYIVINNRPC--KVVEVSTSKPGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGKRLEVFVPSSQKCDVP VYHTDYHLIDICEDGF           DGL L
Sbjct: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPCVYHTDYHLIDICEDGFVSLRSENGNIKDGLRL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR
Sbjct: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 159

[Close]

BLAST of Carg09995 vs. NCBI nr
Match: RVW73616.1 (Eukaryotic translation initiation factor 5A [Vitis vinifera])

HSP 1 Score: 198.7 bits (504), Expect = 4.1e-47
Identity = 103/152 (67.76%), Postives = 114/152 (75.00%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHFDS+A+A   +TYP++A        +  I IK     VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFDSKADAGASKTYPQQAGAIR---KNGYIVIKARPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGFDGLTLPKDENLRRQLK 120
           CHFV IDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGFD L LP D+NL  Q+K
Sbjct: 61  CHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFDDLRLPTDDNLLSQIK 120

Query: 121 EGFAHRKDLVVTVMSAMGEEQICALKDIGPRK 153
           +GFA  KDLVVTVMSAMGEEQICALKDIG  K
Sbjct: 121 DGFADGKDLVVTVMSAMGEEQICALKDIGDMK 149

[Close]

BLAST of Carg09995 vs. NCBI nr
Match: KAD4888908.1 (hypothetical protein E3N88_20981 [Mikania micrantha])

HSP 1 Score: 196.4 bits (498), Expect = 2.1e-46
Identity = 104/162 (64.20%), Postives = 120/162 (74.07%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHF+S+A+A   +TYP++A T + +  H  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGT-IRKGGH--IVIKNRACKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFVSLLTENGNTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+NL  Q+K+GFA  KDLVV+VMSAMGEEQICALKDIGP+
Sbjct: 121 PTDDNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg09995 vs. NCBI nr
Match: XP_022135162.1 (eukaryotic translation initiation factor 5A-2-like [Momordica charantia])

HSP 1 Score: 195.7 bits (496), Expect = 3.5e-46
Identity = 104/162 (64.20%), Postives = 118/162 (72.84%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHFDS+A+A   +T+P++A T      +  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIR---KNGYIVIKNRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+NL  QLK+GFA  KDLV+TVMSAMGEEQICALKDIGP+
Sbjct: 121 PTDDNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 159

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BLAST of Carg09995 vs. ExPASy Swiss-Prot
Match: P24922 (Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=4092 GN=EIF-5A2 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.5e-47
Identity = 102/162 (62.96%), Postives = 117/162 (72.22%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEH F+S+A+A   +TYP++A T + +  H  I IK     VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGT-IRKNGH--IVIKGRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIF GK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+NL  Q+K+GFA  KDLVV+VMSAMGEEQICALKDIGP+
Sbjct: 121 PTDDNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg09995 vs. ExPASy Swiss-Prot
Match: Q945F4 (Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.6e-47
Identity = 101/162 (62.35%), Postives = 115/162 (70.99%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHF+  A+A   +TYP++A T      +  I IK     VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFEPAADAGASKTYPQQAGTIR---KNGYIVIKSRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D++L  Q+K+GFA  KDLVV+VMSAMGEEQICALKDIGP+
Sbjct: 121 PTDDSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg09995 vs. ExPASy Swiss-Prot
Match: Q9AXJ4 (Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 5.6e-47
Identity = 99/162 (61.11%), Postives = 117/162 (72.22%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHF+S+A+A   +T+P++A T      +  I IK+    V+EVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFESKADAGASKTFPQQAGTIR---KNGYIVIKNRPCKVMEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFV IDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTETGNTKDDLRL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P DENL  Q+K+GFA  KDLVV+VMSAMGEE+IC+LKDIGP+
Sbjct: 121 PTDENLLSQIKDGFAEGKDLVVSVMSAMGEERICSLKDIGPK 159

[Close]

BLAST of Carg09995 vs. ExPASy Swiss-Prot
Match: Q9SC12 (Eukaryotic translation initiation factor 5A OS=Senecio vernalis OX=93496 GN=EIFSV1 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.6e-47
Identity = 101/162 (62.35%), Postives = 117/162 (72.22%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEH F+S+A+A   +TYP++A T + +  H  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGT-IRKGGH--IVIKNRACKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+ L  Q+K+GF   KDLVV+VMSAMGEEQICALKDIGP+
Sbjct: 121 PTDDALLTQIKDGFGEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg09995 vs. ExPASy Swiss-Prot
Match: P56337 (Eukaryotic translation initiation factor 5A-5 OS=Solanum tuberosum OX=4113 GN=EIF5A5 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 9.6e-47
Identity = 99/162 (61.11%), Postives = 117/162 (72.22%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHF+S+A+A   +TYP++A T + +  H  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGT-IRKSGH--IVIKNRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIF GK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+ L  Q+K+GFA  KDLV++VMSAMGEEQIC +KDIGP+
Sbjct: 121 PTDDTLLAQVKDGFAEGKDLVLSVMSAMGEEQICGIKDIGPK 159

[Close]

BLAST of Carg09995 vs. ExPASy TrEMBL
Match: A0A438GN25 (Eukaryotic translation initiation factor 5A OS=Vitis vinifera OX=29760 GN=IF5A_3 PE=3 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 2.0e-47
Identity = 103/152 (67.76%), Postives = 114/152 (75.00%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHFDS+A+A   +TYP++A        +  I IK     VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFDSKADAGASKTYPQQAGAIR---KNGYIVIKARPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGFDGLTLPKDENLRRQLK 120
           CHFV IDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGFD L LP D+NL  Q+K
Sbjct: 61  CHFVGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFDDLRLPTDDNLLSQIK 120

Query: 121 EGFAHRKDLVVTVMSAMGEEQICALKDIGPRK 153
           +GFA  KDLVVTVMSAMGEEQICALKDIG  K
Sbjct: 121 DGFADGKDLVVTVMSAMGEEQICALKDIGDMK 149

[Close]

BLAST of Carg09995 vs. ExPASy TrEMBL
Match: A0A5N6NLC9 (Eukaryotic translation initiation factor 5A OS=Mikania micrantha OX=192012 GN=E3N88_20981 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.0e-46
Identity = 104/162 (64.20%), Postives = 120/162 (74.07%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHF+S+A+A   +TYP++A T + +  H  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGT-IRKGGH--IVIKNRACKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVSRTDYQLIDISEDGFVSLLTENGNTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+NL  Q+K+GFA  KDLVV+VMSAMGEEQICALKDIGP+
Sbjct: 121 PTDDNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg09995 vs. ExPASy TrEMBL
Match: A0A251TZ20 (Eukaryotic translation initiation factor 5A OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr09g0270931 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.0e-46
Identity = 101/151 (66.89%), Postives = 117/151 (77.48%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEH F+S+A+A   +TYP++A T + +  H  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGT-IRKGGH--IVIKNHACKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGFDGLTLPKDENLRRQLK 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V   DY LIDI EDGFD L LP D+ L  Q+K
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVSRADYQLIDISEDGFDDLKLPTDDALLTQIK 120

Query: 121 EGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           +GFA  KDLVV+VMSAMGEEQICALKDIGP+
Sbjct: 121 DGFAEGKDLVVSVMSAMGEEQICALKDIGPK 148

[Close]

BLAST of Carg09995 vs. ExPASy TrEMBL
Match: A0A5N6LRB3 (Eukaryotic translation initiation factor 5A OS=Mikania micrantha OX=192012 GN=E3N88_37476 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.7e-46
Identity = 104/161 (64.60%), Postives = 117/161 (72.67%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHF+S+A+A   +T+P++A T      +  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFESKADAGASKTFPQQAGTIR---KNGYIVIKNRACKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTESGGTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGP 151
           P DENL  Q+K+GFA  KDLVVTVMSAMGEEQICALKDIGP
Sbjct: 121 PTDENLLTQIKDGFAEGKDLVVTVMSAMGEEQICALKDIGP 158

[Close]

BLAST of Carg09995 vs. ExPASy TrEMBL
Match: A0A5A7T0H1 (Eukaryotic translation initiation factor 5A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00200 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.7e-46
Identity = 104/162 (64.20%), Postives = 118/162 (72.84%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHFDS+A+A   +T+P++A T      +  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIR---KNGYIVIKNRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIFNGK+LE  VPSS  CDVP+V  TDY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+NL  QLK+GFA  KDLV+TVMSAMGEEQICALKDIGP+
Sbjct: 121 PTDDNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of Carg09995 vs. TAIR 10
Match: AT1G13950.1 (eukaryotic elongation factor 5A-1 )

HSP 1 Score: 172.9 bits (437), Expect = 2.3e-43
Identity = 96/162 (59.26%), Postives = 112/162 (69.14%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSDEEHHF+S ++A   +TYP++A T      +  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDEEHHFES-SDAGASKTYPQQAGTIR---KNGYIVIKNRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIF  K+LE  VPSS  CDVP+V  TDY LIDI EDG+           D L L
Sbjct: 61  CHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P D+ L +Q+K GF   KDLVV+VMSAMGEEQI ALKDIGP+
Sbjct: 121 PNDDTLLQQIKSGFDDGKDLVVSVMSAMGEEQINALKDIGPK 158

[Close]

BLAST of Carg09995 vs. TAIR 10
Match: AT1G69410.1 (eukaryotic elongation factor 5A-3 )

HSP 1 Score: 171.4 bits (433), Expect = 6.6e-43
Identity = 94/162 (58.02%), Postives = 111/162 (68.52%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSD+EHHF+S ++A   +TYP++A   + +  H  I IK     VVEVSTSK GKHGHAK
Sbjct: 1   MSDDEHHFES-SDAGASKTYPQQAGN-IRKGGH--IVIKGRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIF  K+LE  VPSS  CDVP+V   DY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPR 152
           P DE L  QLK GF   KD+VV+VMSAMGEEQ+CALK++GP+
Sbjct: 121 PTDEALLTQLKNGFEEGKDIVVSVMSAMGEEQMCALKEVGPK 158

[Close]

BLAST of Carg09995 vs. TAIR 10
Match: AT1G26630.1 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 161.0 bits (406), Expect = 8.9e-40
Identity = 88/163 (53.99%), Postives = 109/163 (66.87%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSD+EHHF++ + +   +TYP+ A   + +  H  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGN-IRKGGH--IVIKNRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIF  K+LE  VPSS  CDVP+V   DY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKL 120

Query: 121 PKDENLRRQLKEGFAHRKDLVVTVMSAMGEEQICALKDIGPRK 153
           P D+ L  Q++ GF   KD+VV+VMS+MGEEQICA+K++G  K
Sbjct: 121 PTDDGLTAQMRLGFDEGKDIVVSVMSSMGEEQICAVKEVGGGK 159

[Close]

BLAST of Carg09995 vs. TAIR 10
Match: AT1G26630.2 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-27
Identity = 69/130 (53.08%), Postives = 82/130 (63.08%), Query Frame = 0


Query: 1   MSDEEHHFDSRANAREPETYPEEARTWLHQISHKAINIKDLLKDVVEVSTSKPGKHGHAK 60
           MSD+EHHF++ + +   +TYP+ A   + +  H  I IK+    VVEVSTSK GKHGHAK
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGN-IRKGGH--IVIKNRPCKVVEVSTSKTGKHGHAK 60

Query: 61  CHFVAIDIFNGKRLEVFVPSSQKCDVPYVYHTDYHLIDICEDGF-----------DGLTL 120
           CHFVAIDIF  K+LE  VPSS  CDVP+V   DY LIDI EDGF           D L L
Sbjct: 61  CHFVAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKL 120

The following BLAST results are available for this feature:

BLAST of Carg09995 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023445.1	7.2e-100	100.00	Eukaryotic translation initiation factor 5A, partial [Cucurbita argyrosperma sub...	[more]
KAG6589773.1	3.4e-65	81.48	hypothetical protein SDJN03_15196, partial [Cucurbita argyrosperma subsp. sorori...	[more]
RVW73616.1	4.1e-47	67.76	Eukaryotic translation initiation factor 5A [Vitis vinifera]	[more]
KAD4888908.1	2.1e-46	64.20	hypothetical protein E3N88_20981 [Mikania micrantha]	[more]
XP_022135162.1	3.5e-46	64.20	eukaryotic translation initiation factor 5A-2-like [Momordica charantia]	[more]

BLAST of Carg09995 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24922	2.5e-47	62.96	Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=40...	[more]
Q945F4	5.6e-47	62.35	Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa OX=3879 PE=2 SV...	[more]
Q9AXJ4	5.6e-47	61.11	Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV...	[more]
Q9SC12	5.6e-47	62.35	Eukaryotic translation initiation factor 5A OS=Senecio vernalis OX=93496 GN=EIFS...	[more]
P56337	9.6e-47	61.11	Eukaryotic translation initiation factor 5A-5 OS=Solanum tuberosum OX=4113 GN=EI...	[more]

BLAST of Carg09995 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A438GN25	2.0e-47	67.76	Eukaryotic translation initiation factor 5A OS=Vitis vinifera OX=29760 GN=IF5A_3...	[more]
A0A5N6NLC9	1.0e-46	64.20	Eukaryotic translation initiation factor 5A OS=Mikania micrantha OX=192012 GN=E3...	[more]
A0A251TZ20	1.0e-46	66.89	Eukaryotic translation initiation factor 5A OS=Helianthus annuus OX=4232 GN=Hann...	[more]
A0A5N6LRB3	1.7e-46	64.60	Eukaryotic translation initiation factor 5A OS=Mikania micrantha OX=192012 GN=E3...	[more]
A0A5A7T0H1	1.7e-46	64.20	Eukaryotic translation initiation factor 5A OS=Cucumis melo var. makuwa OX=11946...	[more]

BLAST of Carg09995 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13950.1	2.3e-43	59.26	eukaryotic elongation factor 5A-1	[more]
AT1G69410.1	6.6e-43	58.02	eukaryotic elongation factor 5A-3	[more]
AT1G26630.1	8.9e-40	53.99	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]
AT1G26630.2	1.0e-27	53.08	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020189	Translation elongation factor, IF5A C-terminal	SMART	SM01376	eIF_5a_2	coord: 88..146 e-value: 9.1E-4 score: 27.9
IPR020189	Translation elongation factor, IF5A C-terminal	PFAM	PF01287	eIF-5a	coord: 89..146 e-value: 1.3E-10 score: 41.2
IPR001884	Translation elongation factor IF5A-like	PIRSF	PIRSF003025	Transl_init_eIF5A	coord: 1..149 e-value: 3.7E-35 score: 118.7
IPR001884	Translation elongation factor IF5A-like	TIGRFAM	TIGR00037	TIGR00037	coord: 37..145 e-value: 3.4E-22 score: 76.6
IPR001884	Translation elongation factor IF5A-like	PANTHER	PTHR11673	TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER	coord: 1..151
None	No IPR available	GENE3D	2.40.50.140		coord: 89..152 e-value: 1.8E-15 score: 58.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11673:SF43	EUKARYOTIC TRANSLATION INITIATION FACTOR 5A	coord: 1..151
IPR014722	Ribosomal protein L2, domain 2	GENE3D	2.30.30.30		coord: 1..88 e-value: 8.1E-22 score: 78.9
IPR019769	Translation elongation factor, IF5A, hypusine site	PROSITE	PS00302	IF5A_HYPUSINE	coord: 53..60
IPR008991	Translation protein SH3-like domain superfamily	SUPERFAMILY	50104	Translation proteins SH3-like domain	coord: 36..88
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 89..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09995-RA	Carg09995-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045901	positive regulation of translational elongation
biological_process	GO:0045905	positive regulation of translational termination
molecular_function	GO:0043022	ribosome binding
molecular_function	GO:0003746	translation elongation factor activity
molecular_function	GO:0003723	RNA binding