Carg09953 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg09953 vs. NCBI nr
Match: KAG7023484.1 (putative 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 663/663 (100.00%), Postives = 663/663 (100.00%), Query Frame = 0

Query: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60
           MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP
Sbjct: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60

Query: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120
           VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK
Sbjct: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120

Query: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180
           SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY
Sbjct: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180

Query: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240
           TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD
Sbjct: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240

Query: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300
           GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN
Sbjct: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300

Query: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQREAITELLIIAAP 360
           SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQREAITELLIIAAP
Sbjct: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQREAITELLIIAAP 360

Query: 361 SLPCLVAAPSSMAGSNPSSSVQEMGSKAEVPEGYIHKQRDRGAPDAPLMQASVIDIGLLS 420
           SLPCLVAAPSSMAGSNPSSSVQEMGSKAEVPEGYIHKQRDRGAPDAPLMQASVIDIGLLS
Sbjct: 361 SLPCLVAAPSSMAGSNPSSSVQEMGSKAEVPEGYIHKQRDRGAPDAPLMQASVIDIGLLS 420

Query: 421 ASPNSGPELEKLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEA 480
           ASPNSGPELEKLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEA
Sbjct: 421 ASPNSGPELEKLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEA 480

Query: 481 EIEGYGNDKVLSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVC 540
           EIEGYGNDKVLSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVC
Sbjct: 481 EIEGYGNDKVLSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVC 540

Query: 541 EKILKAMGRSLDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLL 600
           EKILKAMGRSLDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLL
Sbjct: 541 EKILKAMGRSLDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLL 600

Query: 601 QDEQVEGLQFLKGDEWFNAPILPDALLVLVGDQVEADIERCASEDVVDCEISHWLERRTR 660
           QDEQVEGLQFLKGDEWFNAPILPDALLVLVGDQVEADIERCASEDVVDCEISHWLERRTR
Sbjct: 601 QDEQVEGLQFLKGDEWFNAPILPDALLVLVGDQVEADIERCASEDVVDCEISHWLERRTR 660

Query: 661 HYV 664
           HYV
Sbjct: 661 HYV 663

BLAST of Carg09953 vs. NCBI nr
Match: KAG6589813.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 621/635 (97.80%), Postives = 621/635 (97.80%), Query Frame = 0

Query: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60
           MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP
Sbjct: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60

Query: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120
           VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK
Sbjct: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120

Query: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180
           SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY
Sbjct: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180

Query: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240
           TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD
Sbjct: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240

Query: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300
           GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN
Sbjct: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300

Query: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQREAITELLIIAAP 360
           SERERISLAVFYLPDWEKEIEPLEKLIDETRPR              REAITELLIIAAP
Sbjct: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPR--------------REAITELLIIAAP 360

Query: 361 SLPCLVAAPSSMAGSNPSSSVQEMGSKAEVPEGYIHKQRDRGAPDAPLMQASVIDIGLLS 420
           SLPCLVAAPSSMAGSNPSSSVQEMGSKAEVPEGYIHKQRDRGAPDAPLMQASVIDIGLLS
Sbjct: 361 SLPCLVAAPSSMAGSNPSSSVQEMGSKAEVPEGYIHKQRDRGAPDAPLMQASVIDIGLLS 420

Query: 421 ASPNSGPELEKLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEA 480
           ASPNSGPELEKLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEA
Sbjct: 421 ASPNSGPELEKLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEA 480

Query: 481 EIEGYGNDKVLSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVC 540
           EIEGYGNDKVLSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVC
Sbjct: 481 EIEGYGNDKVLSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVC 540

Query: 541 EKILKAMGRSLDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLL 600
           EKILKAMGRSLDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLL
Sbjct: 541 EKILKAMGRSLDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLL 600

Query: 601 QDEQVEGLQFLKGDEWFNAPILPDALLVLVGDQVE 636
           QDEQVEGLQFLKGDEWFNAPILPDALLVLVGDQVE
Sbjct: 601 QDEQVEGLQFLKGDEWFNAPILPDALLVLVGDQVE 621

BLAST of Carg09953 vs. NCBI nr
Match: XP_022134811.1 (uncharacterized protein LOC111006992 [Momordica charantia])

HSP 1 Score: 806.2 bits (2081), Expect = 2.1e-229
Identity = 401/627 (63.96%), Postives = 476/627 (75.92%), Query Frame = 0

Query: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79
           MAGTNP+G+VQDVASKGEVPERYIHKE DRGA DAPLM APVID+ LLSS S +GPELEK
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139
           LRHGL SWGCFQ +NHGMS EFL+E+R++AK FF LPME+K K+SREED++EGYGNDMI 
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFR VLHEYTANVKLLSEKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259
           DL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319
            GNEW+NAPI+P ALL+NVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQREAITELLIIAAPSLPCLVAAPSSMAGSNPSS 379
           IEPLEKLI+ET PRLY+TVKNFVGL+FQ     +    AA  +   +  PS+MA      
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKIISVHLRKPSNMAELPDYF 360

Query: 380 SVQEMGSK-----AEVPEGYIHKQ----RDRGAPDAPLMQASVIDIGLLSASPNSGPELE 439
             +    K      + PE YI+K      D      PL +  V+D+  LS+SP S   LE
Sbjct: 361 HTKTFQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE 420

Query: 440 KLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEAEIEGYGNDKV 499
            LR  L +WGC   INH +S  FL  +  +  QFF+ PMEEK K   E   +EGYG D V
Sbjct: 421 DLRLALTSWGCFQAINHSISSSFLNKIHQISNQFFSLPMEEKNKCCRELYGLEGYGTDMV 480

Query: 500 LSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVCEKILKAMGRS 559
            S+ QILDW  RL+L + PE+ R++K+WP NP  FRE + E++  ++ + E +L AM RS
Sbjct: 481 FSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARS 540

Query: 560 LDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLLQDEQVEGLQF 619
           +++E NSF EQ G+R EL  RFN YPPC  PDLVLG K H+D SAIT++L D +VEGLQ+
Sbjct: 541 INVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQW 600

Query: 620 LKGDEWFNAPI--LPDALLVLVGDQVE 636
            K D+WF  P+  + D+LL+ +G+Q E
Sbjct: 601 RKDDQWFRVPVPAMADSLLINIGEQAE 627

BLAST of Carg09953 vs. NCBI nr
Match: XP_022960723.1 (protein SRG1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 703.4 bits (1814), Expect = 1.9e-198
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60
           MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP
Sbjct: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60

Query: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120
           VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK
Sbjct: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120

Query: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180
           SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY
Sbjct: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180

Query: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240
           TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD
Sbjct: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240

Query: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300
           GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN
Sbjct: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300

Query: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQ 348
           SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQ
Sbjct: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQ 347

BLAST of Carg09953 vs. NCBI nr
Match: XP_023516521.1 (protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.4 bits (1700), Expect = 3.2e-185
Identity = 321/328 (97.87%), Postives = 326/328 (99.39%), Query Frame = 0

Query: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79
           MAGTNPSGTVQ VASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSS+SGPELEK
Sbjct: 1   MAGTNPSGTVQGVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSKSGPELEK 60

Query: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139
           LRHGLQSWGCFQVVNHGMSAEFLDE+RRLAKQFFDLPMEEKSKYSREE+EIEGYGNDMIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199
           SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFR VLHEYTANVKLLSEKILKAMAISL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259
           DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319
           NGNEWFNAPIVPGALL+NVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDW+KE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWKKE 300

Query: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQ 348
           IEPLEKLIDETRPRLYKTVKNFVGLYFQ
Sbjct: 301 IEPLEKLIDETRPRLYKTVKNFVGLYFQ 328

BLAST of Carg09953 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 1.1e-58
Identity = 119/323 (36.84%), Postives = 180/323 (55.73%), Query Frame = 0

Query: 25  PSGTVQDVASKG--EVPERYIHKESDRGARDAPLMAA----PVIDMALLSSSSESGPELE 84
           P  +VQ ++  G   VP RY+     R   +     A    PV+DM  +    E    L 
Sbjct: 8   PIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEG---LR 67

Query: 85  KLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMI 144
            +R   + WG FQ+VNHG++   ++ +R   ++FF+LP+EEK KY+   D  EGYG+ + 
Sbjct: 68  LVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLG 127

Query: 145 LSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAIS 204
           +     LDW+D  +L   P        WP+ P + R ++ +Y   V+ L E++ + ++ S
Sbjct: 128 VVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSES 187

Query: 205 LDLNEDSFIKQY--GEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGL 264
           L L  +  ++    G++V    R NFYP+C  P L LG+  H+D   ITILL DE+V GL
Sbjct: 188 LGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGL 247

Query: 265 QFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDW 324
           Q   G+ W     VP AL++N+GDQ +I SNGI+KS  H+V+ NS  ER+SLA FY P  
Sbjct: 248 QVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRS 307

Query: 325 EKEIEPLEKLIDETRPRLYKTVK 340
           +  + P+E+L+   RP LYK ++
Sbjct: 308 DIPVGPIEELVTANRPALYKPIR 327

BLAST of Carg09953 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.4e-58
Identity = 114/317 (35.96%), Postives = 183/317 (57.73%), Query Frame = 0

Query: 38  VPERYIHKESDRGARDAPL---MAAPVIDMALLSSSSESGPELEKLRHGLQSWGCFQVVN 97
           VP RY+  + D+   D      +  P+IDM  L SS+    E+EKL    + WG FQ+VN
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 98  HGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMILSNQQILDWTDRLYLT 157
           HG+ + FLD+++   + FF+LPMEEK K+ +  DEIEG+G   ++S  Q LDW D  + T
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 158 VYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEV 217
           V P E  +   +P  P  FR  L  Y++ V+ +++ ++  MA +L++  +   K + +  
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVD 208

Query: 218 KLDA-RFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFLNGNEWFNAPIVPGA 277
            + + R N+YP C  PD V+G+ PH+D   +T+L+Q  +VEGLQ     +W     +P A
Sbjct: 209 SVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

Query: 278 LLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKEIEPLEKLIDETRPR 337
            ++N+GD  EI +NG ++S  HR + NSE+ER+S+A F+     KE+ P + L++  +  
Sbjct: 269 FIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVA 328

Query: 338 LYK--TVKNFVGLYFQR 349
            +K  T+K +    F R
Sbjct: 329 RFKRLTMKEYNDGLFSR 345

BLAST of Carg09953 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.1e-55
Identity = 117/319 (36.68%), Postives = 174/319 (54.55%), Query Frame = 0

Query: 25  PSGTVQDVASK--GEVPERYIHKESDR--GARDAPLMA-APVIDMALLSSSSESGPE--L 84
           P   VQ +A      +P+RYI   S R     DAP     P+ID+  L S      +  +
Sbjct: 23  PIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIM 82

Query: 85  EKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDM 144
            ++    + WG FQVVNHG+  E +D  R   ++FF +P+  K  YS      EGYG+ +
Sbjct: 83  ARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRL 142

Query: 145 ILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAI 204
            +     LDW+D  +L + P     F  WP+ P   R V+ EY   +  LS +I++ ++ 
Sbjct: 143 GVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLST 202

Query: 205 SLDLNEDSFIKQY-GEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGL 264
           +L L ED F + + GE +    R N+YP+C  P+L LG+ PH+D   +TILL D++V GL
Sbjct: 203 NLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGL 262

Query: 265 QFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDW 324
           Q    + W      P A ++N+GDQ +I SN  +KS  HRV+ NS++ER+SLA FY P  
Sbjct: 263 QVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKS 322

Query: 325 EKEIEPLEKLIDETRPRLY 336
           +  I+PL++L+    P LY
Sbjct: 323 DIPIQPLQELVSTHNPPLY 341

BLAST of Carg09953 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 9.3e-55
Identity = 115/321 (35.83%), Postives = 174/321 (54.21%), Query Frame = 0

Query: 25  PSGTVQDVASKG--EVPERYIHKESDRGAR-------DAPLMAAPVIDMALLSSSSESGP 84
           P   VQ +A      +P+RYI   S R          +   +  P+ID+  L S +E   
Sbjct: 52  PIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNED-- 111

Query: 85  ELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGN 144
           + +++    + WG FQV+NHG+  E +D  R   K FF+LP+E K  YS      EGYG+
Sbjct: 112 DKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYGS 171

Query: 145 DMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAM 204
            + +    ILDW D  YL   P     F  WP+ P   R +  EY   +  L  +++  +
Sbjct: 172 RLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTIL 231

Query: 205 AISLDLNEDSFIKQY-GEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVE 264
           + +L L  +   + + GE+V    R N+YP+C  P+L LG+ PH+D   +TILL D++V 
Sbjct: 232 SSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQVV 291

Query: 265 GLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLP 324
           GLQ  +G+ W     +  A ++N+GDQ +I SN  +KS  HRV+ NSE+ER+SLA FY P
Sbjct: 292 GLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFYNP 351

Query: 325 DWEKEIEPLEKLIDETRPRLY 336
             +  I+P+++L+  T P LY
Sbjct: 352 KSDIPIQPMQQLVTSTMPPLY 370

BLAST of Carg09953 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.2e-54
Identity = 112/321 (34.89%), Postives = 174/321 (54.21%), Query Frame = 0

Query: 38  VPERYIHKESDRGA---RDAPLMAAPVIDMALLSSSSESGPELEKLRHGLQSWGCFQVVN 97
           +PERYI  E+            MA P+ID+  L     S  E  KLR   Q WG F ++N
Sbjct: 29  IPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLLIN 88

Query: 98  HGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMILSNQQILDWTDRLYLT 157
           HG+  E +  ++R    FF  P++ K +Y++  + +EGYG   + S  Q LDW D LYL 
Sbjct: 89  HGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLYLH 148

Query: 158 VYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEV 217
           V+P +S   ++WPT+P  FR  +  Y++  K L+  + + MA ++    +S +  + EE 
Sbjct: 149 VHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLF-EEQ 208

Query: 218 KLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFLNGNEWFNAPIVPGAL 277
               R  +YP CR  D V+G+ PH+D   +T+LL+   V+GLQ     +WF+     GAL
Sbjct: 209 PRGLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAPNGAL 268

Query: 278 LINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKEIEPLEKLIDETRPRL 337
           + N+GD  EI SNG F+S  HR + N  +ERIS A+F+ P     I PL + + + + + 
Sbjct: 269 IANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKDGKVK- 328

Query: 338 YKTVKNFVGLYFQREAITELL 356
           Y+++     L F ++  T+ L
Sbjct: 329 YRSISY---LDFMKQIFTQQL 344

BLAST of Carg09953 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 1.0e-229
Identity = 401/627 (63.96%), Postives = 476/627 (75.92%), Query Frame = 0

Query: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79
           MAGTNP+G+VQDVASKGEVPERYIHKE DRGA DAPLM APVID+ LLSS S +GPELEK
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139
           LRHGL SWGCFQ +NHGMS EFL+E+R++AK FF LPME+K K+SREED++EGYGNDMI 
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199
           SNQQILDWTDRLYLTV P+ES RFKYWPTNPERFR VLHEYTANVKLLSEKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259
           DL+E+SF+ QYG+ V+LDARFNFY RCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319
            GNEW+NAPI+P ALL+NVGDQ EITSNGIFKS VHRVLTNSERERISLAVFYLPD +KE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQREAITELLIIAAPSLPCLVAAPSSMAGSNPSS 379
           IEPLEKLI+ET PRLY+TVKNFVGL+FQ     +    AA  +   +  PS+MA      
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKIISVHLRKPSNMAELPDYF 360

Query: 380 SVQEMGSK-----AEVPEGYIHKQ----RDRGAPDAPLMQASVIDIGLLSASPNSGPELE 439
             +    K      + PE YI+K      D      PL +  V+D+  LS+SP S   LE
Sbjct: 361 HTKTFQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE 420

Query: 440 KLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEAEIEGYGNDKV 499
            LR  L +WGC   INH +S  FL  +  +  QFF+ PMEEK K   E   +EGYG D V
Sbjct: 421 DLRLALTSWGCFQAINHSISSSFLNKIHQISNQFFSLPMEEKNKCCRELYGLEGYGTDMV 480

Query: 500 LSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVCEKILKAMGRS 559
            S+ QILDW  RL+L + PE+ R++K+WP NP  FRE + E++  ++ + E +L AM RS
Sbjct: 481 FSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARS 540

Query: 560 LDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLLQDEQVEGLQF 619
           +++E NSF EQ G+R EL  RFN YPPC  PDLVLG K H+D SAIT++L D +VEGLQ+
Sbjct: 541 INVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQW 600

Query: 620 LKGDEWFNAPI--LPDALLVLVGDQVE 636
            K D+WF  P+  + D+LL+ +G+Q E
Sbjct: 601 RKDDQWFRVPVPAMADSLLINIGEQAE 627

BLAST of Carg09953 vs. ExPASy TrEMBL
Match: A0A6J1H9T6 (protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 9.2e-199
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60
           MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP
Sbjct: 1   MISVLQPEQQNSVASAPPAMAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAP 60

Query: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120
           VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK
Sbjct: 61  VIDMALLSSSSESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEK 120

Query: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180
           SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY
Sbjct: 121 SKYSREEDEIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEY 180

Query: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240
           TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD
Sbjct: 181 TANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHAD 240

Query: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300
           GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN
Sbjct: 241 GSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTN 300

Query: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQ 348
           SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQ
Sbjct: 301 SERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQ 347

BLAST of Carg09953 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 2.1e-182
Identity = 318/328 (96.95%), Postives = 323/328 (98.48%), Query Frame = 0

Query: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79
           MAGTNPSGTVQDVASKGEVPERYIH ESDRGA DAPLMAAPVIDMALLSS S+SGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139
           LRHGLQSWGCFQVVNHGMSAEFLDE+RRLAKQFFDLPMEEKSKYSREE+EIEGYGNDMIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199
           SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFR VLHEYTANVKLLSEKILKAMAISL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259
           DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319
           NGNEWFNAPIVPGALL+NVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPD EKE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQ 348
           IEPLEKLIDETRPRLYK+VKNFVGLYFQ
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQ 328

BLAST of Carg09953 vs. ExPASy TrEMBL
Match: A0A6J1JB89 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 2.4e-178
Identity = 318/352 (90.34%), Postives = 323/352 (91.76%), Query Frame = 0

Query: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79
           MAGTNPSGTVQDVASKGEVPERYIH ESDRGA DAPLMAAPVIDMALLSS S+SGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139
           LRHGLQSWGCFQVVNHGMSAEFLDE+RRLAKQFFDLPMEEKSKYSREE+EIEGYGNDMIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERF------------------------RA 199
           SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERF                        R 
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRYFSQTFTIFRRICRNELIRDEHCRE 180

Query: 200 VLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGV 259
           VLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGV
Sbjct: 181 VLHEYTANVKLLSEKILKAMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGV 240

Query: 260 KPHADGSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVH 319
           KPHADGSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALL+NVGDQAEITSNGIFKSPVH
Sbjct: 241 KPHADGSAITILLQDEEVEGLQFLNGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVH 300

Query: 320 RVLTNSERERISLAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQ 348
           RVLTNSERERISLAVFYLPD EKEIEPLEKLIDETRPRLYK+VKNFVGLYFQ
Sbjct: 301 RVLTNSERERISLAVFYLPDSEKEIEPLEKLIDETRPRLYKSVKNFVGLYFQ 352

BLAST of Carg09953 vs. ExPASy TrEMBL
Match: A0A498KDN2 (Uncharacterized protein OS=Malus domestica OX=3750 GN=DVH24_017710 PE=4 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 2.6e-169
Identity = 318/635 (50.08%), Postives = 418/635 (65.83%), Query Frame = 0

Query: 20  MAGTNPSGTVQDVASKGE-VPERYIHKESDRGARD-APLMA--APVIDMALLSSSS---- 79
           MA TN    VQ+++  GE  P  YI KES  G+ D +P +A   P+ID++L S SS    
Sbjct: 1   MAMTN----VQEMSMNGEQPPAEYIVKESRFGSIDSSPPLADEIPIIDISLFSPSSPQYS 60

Query: 80  --ESGPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREED- 139
                 EL+KLR  L S GCFQ   HG+S   LD++R  AKQFF LP+EEK KYSR  D 
Sbjct: 61  EQAENHELQKLRSALSSAGCFQATGHGISDSLLDQVREAAKQFFALPVEEKEKYSRTIDG 120

Query: 140 EIEGYGNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLS 199
             EGYGND+I+S++Q+LDW+ RL L V+P++  +   WP  P  F  +LHEYT  +KL+ 
Sbjct: 121 GFEGYGNDVIVSDKQVLDWSYRLILRVFPQDQRKLHLWPQIPNDFGEMLHEYTTKIKLMM 180

Query: 200 EKILKAMAISLDLNEDSFIKQ-YGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITIL 259
             + KAMA SL+L E SF  Q  G++  + ARFNFYP C   DLVLGVKPH D S +T L
Sbjct: 181 GLVFKAMAKSLNLEEKSFATQLLGDQSLMQARFNFYPPCSRSDLVLGVKPHTDRSGVTCL 240

Query: 260 LQDEEVEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERIS 319
           LQD++VEGLQ L   +W   PIVP A+++N+GDQ +I SNGI+KSP+HRV+TN E  R+S
Sbjct: 241 LQDKDVEGLQVLIDGKWVRVPIVPHAIVLNLGDQMQIMSNGIYKSPMHRVVTNGESMRLS 300

Query: 320 LAVFYLPDWEKEIEPLEKLIDETRPRLYKTVKNFVGLYFQREAITELLIIAAPSLPCLVA 379
           +A+F  PD E EI P+E LIDETRPRLYK VKN+  + ++     E   IA  +L     
Sbjct: 301 VALFNEPDPETEIGPVEDLIDETRPRLYKNVKNYGRINYECYQRGE---IALETLKLQNL 360

Query: 380 APSSMAGSNPSSSVQEMGSKAEVPEGYIHKQRDRGAP-------DAPLMQASVIDIGLLS 439
               + GS     V   G   +VP+ YI K  D G P        A LM   +ID+ LL+
Sbjct: 361 VKMELLGSKTVQEVLIQGE--QVPDTYIRKVEDGGVPVPVPDASAAQLMDVPIIDLALLA 420

Query: 440 ASPNSGPELEKLRHGLQTWGCILGINHGMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEA 499
           AS  +  ELE+ R  L TWGC   INH MSPEFL++VR + KQFFA P+ +K KY  +  
Sbjct: 421 ASSITVDELEEFRSALATWGCFQVINHSMSPEFLDEVREITKQFFALPVADKKKYLRQVN 480

Query: 500 EIEGYGNDKVLSDTQILDWNHRLFLTLLPEESRRVKHWPSNPHRFREVIDEYSANMRVVC 559
           +I+GYGND V S+ Q LDW+ RL+L++ P+++R++K WP NP  F   +D+Y+  ++VV 
Sbjct: 481 DIQGYGNDMVFSEQQTLDWSDRLYLSVYPQDNRKLKFWPKNPDSFSRTLDQYAMELQVVT 540

Query: 560 EKILKAMGRSLDLEENSFVEQFGERFELAARFNLYPPCPNPDLVLGAKPHADVSAITVLL 619
           + ILK M RSLDLE+N F + +GE+ ++  RFN YPPCP PDLVLG KPH+D + IT++L
Sbjct: 541 KTILKVMTRSLDLEDNCFSDLYGEQGKMNIRFNFYPPCPRPDLVLGLKPHSDGTLITLVL 600

Query: 620 QDEQVEGLQFLKGDEWFNAPILPDALLVLVGDQVE 636
           QD++VEGLQFLKGD WF API+PDALL+ +GD VE
Sbjct: 601 QDKEVEGLQFLKGDHWFRAPIVPDALLINIGDLVE 626

BLAST of Carg09953 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 374.8 bits (961), Expect = 1.4e-103
Identity = 183/327 (55.96%), Postives = 233/327 (71.25%), Query Frame = 0

Query: 28  TVQDVASKGE-VPERYIHKESDRGA-----RDAPLMAAPVIDMALLSSSSESG-PELEKL 87
           TVQ+V + GE +PERY+H  +  G         P M  P ID+ LL SSSE+G  EL KL
Sbjct: 8   TVQEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKL 67

Query: 88  RHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMILS 147
              L +WG  QV+NHG++  FLD+I +L K+FF LP EEK K +RE D I+GYGNDMIL 
Sbjct: 68  HSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILW 127

Query: 148 NQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISLD 207
           + Q+LDW DRLY+T YP++  +  +WP  P  FR  LHEYT   +++ E+  KAMA SL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLE 187

Query: 208 LNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFLN 267
           L E+SF+  YGE   LD RFN YP C +PD V+GVKPHADGSAIT+LL D++V GLQF  
Sbjct: 188 LEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQK 247

Query: 268 GNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKEI 327
             +W+ APIVP  +LINVGDQ EI SNGI+KSPVHRV+TN E+ERIS+A F +P  +KEI
Sbjct: 248 DGKWYKAPIVPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADKEI 307

Query: 328 EPLEKLIDETRPRLYKTVKNFVGLYFQ 348
           +P+ +L+ E RPRLYKTVK +V LYF+
Sbjct: 308 QPVNELVSEARPRLYKTVKKYVELYFK 334

BLAST of Carg09953 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 362.8 bits (930), Expect = 5.7e-100
Identity = 175/327 (53.52%), Postives = 231/327 (70.64%), Query Frame = 0

Query: 28  TVQDVASKGE-VPERYIHKESDRGARD-----APLMAAPVIDMALLSSSSESG-PELEKL 87
           TVQ+V + G+ +PERY+H  +  G         P M  P ID++LL SSS  G  E++KL
Sbjct: 8   TVQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKL 67

Query: 88  RHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMILS 147
              L +WG  QV+NHG++  FLD+I +L KQFF LP EEK K +RE   I+GYGNDMILS
Sbjct: 68  HSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILS 127

Query: 148 NQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISLD 207
           + Q+LDW DRL+LT YP++  + K+WP  P  F   L EYT   ++L EK  KAMA SL+
Sbjct: 128 DNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLE 187

Query: 208 LNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFLN 267
           L E+ F++ YGE   +++RFNF+P C  PD V+G+KPHADGSAIT+LL D++VEGLQFL 
Sbjct: 188 LEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLK 247

Query: 268 GNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKEI 327
             +W+ APIVP  +LI +GDQ EI SNGI+KSPVHRV+TN E+ERIS+A F +P  +KEI
Sbjct: 248 DGKWYKAPIVPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDKEI 307

Query: 328 EPLEKLIDETRPRLYKTVKNFVGLYFQ 348
            P + L+ E RPRLYKTV  +V L+++
Sbjct: 308 HPADGLVTEARPRLYKTVTKYVDLHYK 334

BLAST of Carg09953 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 2.2e-96
Identity = 173/325 (53.23%), Postives = 225/325 (69.23%), Query Frame = 0

Query: 28  TVQDVASKGE-VPERYIHKES--DRGAR---DAPLMAAPVIDMALLSSSSESG-PELEKL 87
           TVQ+V + GE +PERY+   +  D G       P+M  P ID++LL S S+ G  EL KL
Sbjct: 8   TVQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKL 67

Query: 88  RHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMILS 147
              L +WG  QV+NHG++   LD+I +L K+F  LP EEK KY+RE   I+GYGNDMIL 
Sbjct: 68  HSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILW 127

Query: 148 NQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISLD 207
           + Q+LDW DRLY+T YP++  + K+WP  P  FR  LHEYT    L+  ++ KAMAISL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLE 187

Query: 208 LNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFLN 267
           L E+ F+   GE   +D RFN YP C  PD V+GV+PHAD SA T+LL D+ VEGLQFL 
Sbjct: 188 LEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLK 247

Query: 268 GNEWFNAPIVPG-ALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 327
             +W+ AP+V    +LINVGDQ EI SNGI+KSPVHRV+TN+E+ERIS+A F +P  +KE
Sbjct: 248 DGKWYKAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADKE 307

Query: 328 IEPLEKLIDETRPRLYKTVKNFVGL 345
           I+P++ L+ E RPRLYK VKN+V L
Sbjct: 308 IQPVDGLVSEARPRLYKPVKNYVDL 332

BLAST of Carg09953 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 345.9 bits (886), Expect = 7.2e-95
Identity = 172/342 (50.29%), Postives = 225/342 (65.79%), Query Frame = 0

Query: 28  TVQDVASKGE-VPERYIHKESDRGARDAPL------MAAPVIDMALLSSSSESG-PELEK 87
           TVQ+V + GE +PERY++  +  G  D P       M   +ID+ LL SSS+ G  EL K
Sbjct: 8   TVQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSK 67

Query: 88  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 147
           L   + +WG  QV+NHG+S   LD+I  L KQFF LP +EK KY+RE    +G+GNDMIL
Sbjct: 68  LHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMIL 127

Query: 148 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 207
           S+ Q+LDW DRLYL  YP++  + K+WP NP  FR  LHEYT   +L+ EK  KA+A SL
Sbjct: 128 SDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSL 187

Query: 208 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 267
           +L ++ F++ +GE   L+ RFN YP C  PD VLG+KPH+DGSA T++L D+ VEGLQFL
Sbjct: 188 ELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFL 247

Query: 268 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 327
              +W+ A I+P  +LINVGD  E+ SNGI+KSPVHRV+ N ++ERI +A F   D +KE
Sbjct: 248 KDGKWYKASILPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKE 307

Query: 328 IEPLEKLIDETRPRLYKTV----KNFVGLYFQREAITELLII 358
           I+PL  L+ E RPRLYK V    KNF   Y Q     E  +I
Sbjct: 308 IQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

BLAST of Carg09953 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 4.7e-70
Identity = 135/326 (41.41%), Postives = 198/326 (60.74%), Query Frame = 0

Query: 27  GTVQDV-----ASKGEVPERYIHKESDRGARDAPLMA------APVIDMALLS--SSSES 86
           G + DV     +   +VPER+I +E +RG   + L         PVID++ LS   + + 
Sbjct: 12  GKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDF 71

Query: 87  GPELEKLRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGY 146
             E+ KL    + WG FQV+NHG+  E +++I  +A +FFD+P+EEK KY  E   ++GY
Sbjct: 72  FFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGY 131

Query: 147 GNDMILSNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILK 206
           G   I S  Q LDW +   L V+P +    K WP+ P RF   L  Y+  ++ L +++LK
Sbjct: 132 GQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLK 191

Query: 207 AMAISLDLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDE-E 266
            +AISL L E+ F + +GE V+   R N+YP C +PDLVLG+ PH+DGSA+T+L Q +  
Sbjct: 192 YIAISLGLKEERFEEMFGEAVQA-VRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNS 251

Query: 267 VEGLQFLNGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFY 326
             GLQ L  N W     +P AL+IN+GD  E+ SNG +KS  HR +TN E+ER+++  FY
Sbjct: 252 CVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFY 311

Query: 327 LPDWEKEIEPLEKLI-DETRPRLYKT 338
            P++E EIEP+ +L+ DET P  Y++
Sbjct: 312 APNYEVEIEPMSELVDDETNPCKYRS 336