Carg09833 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09833
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RNA methyltransferase
Location	Carg_Chr10: 2985697 .. 2988957 (-)
RNA-Seq Expression	Carg09833
Synteny	Carg09833

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTGGTTCCTCCATTGCATGCTGCCACCGCTTCCTCCGCCGGGGATTACCGTCGTTCATCGACGTTTCGTATTCACCTCAGAGAGTATCAGCAAAAGAGATAATTAAAGTTGCGATTTAAGGAGATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGGTATTTCTCCTGACCTATTCTTCCACACTTACCTCGGAGTTCTAGGTTGCCCTTCTTGGGTCTTCACGTGGTAAATATAGTTCTGAGAAGTATTCTCGATTGTCTCCGGTTTCTGGTTAAGATTCGAACTATTATCGGTTAGTGATAGAAACGAGAATTTAAAATTTCGAGAATTGGTGTAGACCAGTCAAGCTTGTTTTTCCAACTTCAGTGCTCTAATAATTTATGATTCGTTCTTATTCGGCCGACAGAATGTGCGTTACCCCTAGTTTCGTTTTCTTATTTTTTGTGTGGCAGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGGTAGCAATACTTCAATCTCAGTATTTATATATATATATATATATATATATATATATATATATATATATTTAAAAAAAAAAAAAAAAGGATTGATACGTCCTTATCATGTGCCGTAATTTTATGAACTTCTTACTCATTTTACCAAAGGATATGCATGGAAGTCTCAAGTCTGAAAAGAAGTTTATATGCATGTTGCAGCAAAGACAATAAAACTATAGCAACTTCTTGTCATTTCGCTATGAACTTAAAACTGTCTTTTGTGCATCTCAGTTCAAGAAAGCTCTGTTATCACTGGCTATTTTATTGTTCATTGAAGATTGTGGAAGTGCTTTTTCTCAAATACTAGAGCTCTTTACTTATGCGGATAACTTTCTTTTCGCAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGGTAAGATTTACTCTAAAAGCTCTACAACTTCCATTGCAGATCCACTTTATACTTCTACAAAGTACATGAAAGCGGTTTTGTGAAAAACTATCTTCTTTGGAGGTCTAAATCTTAATTGAGGATGCATTCTATCATTAACTATTTTAAGCACTTGTAAATTAGTCATGTAAATTATAGCCTGGGATGGTTGGATGCTGATTTATTGTTCGACTATATTATTAGGGAGATTAGTTGGAGAATTCTTATACAAAAAAAGTAATTGAAGGGTTTTACAGAAAAGGGTAGATATTAAAGGAGGTTGTTGATAAGGGTATGTGTGATAAATATTTAGATCTAAATAGTCTTGAGTGGTATGCCCTTTCCGGGTCCCCTATGGGCTGTAATTATAACCTGTAACACACATATTTTTTTGGCTTTGTCCTATCGGATTGCCTCTTAGTAACTTACTTCATGGGTGGGAACTCAAATCTCTGTTCCTCCTTTATATTGATAGAATCAAAGCTCGTGACCTGAAATTATTGAGGGAACTTACATTGTTTGTTGAACTACATGGATCATAGTGACTTCATCAATACGAACTCTATCTTTTACTGGTGGCTTTAGTAATAATCACATCTGTTCTGGTAGTTATGTTAGGAGAAAATATATCTCAAAAGTGAAAGAATTGTTTGTATACCAAGGTACTTAGCCAGAGTTGATCTCCCAGTCTTTCTATACAAATGATTGCTGAGTTTCCAACCTGAACTTCTCTTTATGCAAGATCGAGTTGACGATGCTTACCGGAATCTAAGAAGGGCAAGAATAAATGTTGGGAAGCCGGATAATGGTTTGGAGCTTGGTGCTTCTGGAGCTTCGAAGATAGATACAGAAGCTTCTACAAGTATTTCTTCTTCCAAAGAACATGATCTTCATACCGATGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGGTAAGTTAATCTTTTTATCTTGTGTTGCGTATTAATACTATTTGTTTTCTTGACCCTTGATGCGTCTCCCTTCCCCTTCTGGGGTCACAATTTGCGTTTCTTTTTTTCTTTCTGTACTGCAGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGGTACTTGCCTTGTCATACACCTATGCACTTTGTGTTAAATGGTCCTAAGATCATGAATAAGAACCTTTTGTATTTACTTTAATGTTCGATTAAGAACCTTCTTAATTATGCATTCATTCTCAAAAAGGCAGGGTGGCATTCTTGTTTTGGAGCCTCCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGGTAAAAAATTTCATGCATCCAAATTGTTAATCAACAATTACATTTAGTCAAACTCAAAATTTTTTTTGAGTGCTCTTCTAGCATCTGCTATCATATTGCCTTAGATTGCTGATCTTTCTAATGCAGACAACTAAAAAGAACTTCAAGTCTATAAGGATCCTTCCAGAAGAGTTCCAAGAGATACTCCTAGACAAGGTATTTTTATGATTTATTTGGCTTAGTTTTACAAACAGCATCAGTTTTCACTAACTTCTGGACAAAATACCACCCCAGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGAATGTTTGTAATCTGGACATCTGAACCAAATTAAATGCAGAGAGCCATCAGCTGGTCTTTAGAAGTAGTTAGTTACAGGCCTTGTTTAGTTTAAATAGTACATTACATACTCCAGACATGGTAAAGAGTGGCTGTTTTTGAAGGGTGCCGTCTTTAGTGGGATCCCTGTCACTTATGCGTAGGTGCTTTTTGAAGGCTTGTTGTAAGCTTCTGCTTTTTGATATTTGTCGTTATCCTTTTGAACAATCTGAACGCTCTTATATATTACTATTCATGTACCGTTCATTTATCATTTTCACTCATAACTAAGTTTAAACCTTTCTCTGTGTGACAACAAAGTTGTTTGTCTCGTTTTGTAACCCGAACAAGGTGACAGTTGATGAAAATGTAATCAAAATAAGTTTTTTAATTCTGCTCAGTTTGTATCA

mRNA sequence

TATTGGTTCCTCCATTGCATGCTGCCACCGCTTCCTCCGCCGGGGATTACCGTCGTTCATCGACGTTTCGTATTCACCTCAGAGAGTATCAGCAAAAGAGATAATTAAAGTTGCGATTTAAGGAGATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGATCTAAATAGTCTTGAGTGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGAATGTTTGTAATCTGGACATCTGAACCAAATTAAATGCAGAGAGCCATCAGCTGGTCTTTAGAAGTAGTTAGTTACAGGCCTTGTTTAGTTTAAATAGTACATTACATACTCCAGACATGGTAAAGAGTGGCTGTTTTTGAAGGGTGCCGTCTTTAGTGGGATCCCTGTCACTTATGCGTAGGTGCTTTTTGAAGGCTTGTTGTAAGCTTCTGCTTTTTGATATTTGTCGTTATCCTTTTGAACAATCTGAACGCTCTTATATATTACTATTCATGTACCGTTCATTTATCATTTTCACTCATAACTAAGTTTAAACCTTTCTCTGTGTGACAACAAAGTTGTTTGTCTCGTTTTGTAACCCGAACAAGGTGACAGTTGATGAAAATGTAATCAAAATAAGTTTTTTAATTCTGCTCAGTTTGTATCA

Coding sequence (CDS)

ATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGATCTAAATAGTCTTGAGTGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGA

Protein sequence

MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARKFNCRRILGVDIDPDLNSLECLSVAKWIHLNWGDDGLITLFYKIWRLLHPIGFRTVEALTTGLSHSNTGGFNRPIFVFQK

Homology

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BLAST of Carg09833 vs. NCBI nr
Match: KAG7023603.1 (putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 332.4 bits (851), Expect = 2.1e-87
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDLNSLECLSVAKWIHLNWGDDGLITLFYKI 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDLNSLECLSVAKWIHLNWGDDGLITLFYKI
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDLNSLECLSVAKWIHLNWGDDGLITLFYKI 120

Query: 121 WRLLHPIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 157
           WRLLHPIGFRTVEALTTGLSHSNTGGFNRPIFVFQK
Sbjct: 121 WRLLHPIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 156

[Close]

BLAST of Carg09833 vs. NCBI nr
Match: KAG6589938.1 (putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 271.6 bits (693), Expect = 4.4e-69
Identity = 154/271 (56.83%), Postives = 154/271 (56.83%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDL-------------------NSLE----- 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPD                    N LE     
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ----------------------------------------------CLSVAKWIHLNWGD 157
                                                         CLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

[Close]

BLAST of Carg09833 vs. NCBI nr
Match: XP_023516346.1 (probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271.6 bits (693), Expect = 4.4e-69
Identity = 154/271 (56.83%), Postives = 154/271 (56.83%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDL-------------------NSLE----- 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPD                    N LE     
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ----------------------------------------------CLSVAKWIHLNWGD 157
                                                         CLSVAKWIHLNWGD
Sbjct: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

[Close]

BLAST of Carg09833 vs. NCBI nr
Match: XP_022961298.1 (probable RNA methyltransferase At5g51130 [Cucurbita moschata])

HSP 1 Score: 271.6 bits (693), Expect = 4.4e-69
Identity = 154/271 (56.83%), Postives = 154/271 (56.83%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDL-------------------NSLE----- 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPD                    N LE     
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ----------------------------------------------CLSVAKWIHLNWGD 157
                                                         CLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

[Close]

BLAST of Carg09833 vs. NCBI nr
Match: XP_022987832.1 (probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita maxima])

HSP 1 Score: 266.9 bits (681), Expect = 1.1e-67
Identity = 151/271 (55.72%), Postives = 153/271 (56.46%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEK EAELKQNPKRRKKHAFPYGNY+SYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKTEAELKQNPKRRKKHAFPYGNYRSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDL-------------------NSLE----- 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPD                    N LE     
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ----------------------------------------------CLSVAKWIHLNWGD 157
                                                         CLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

[Close]

BLAST of Carg09833 vs. ExPASy Swiss-Prot
Match: Q6NPC9 (Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g51130 PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 7.8e-37
Identity = 96/279 (34.41%), Postives = 120/279 (43.01%), Query Frame = 0


Query: 3   EEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLD 62
           ++Q+  +    Q+ K++ +  +P+GNY++YYGYR+     EDPRLKV KKEWFEGKDCLD
Sbjct: 41  KQQQGQQGNCNQSKKKKNQEVYPFGNYRNYYGYRISNDTDEDPRLKVLKKEWFEGKDCLD 100

Query: 63  IGCNSGMVTIQIARKFNCRRILGVDIDPD------------------------------- 122
           IGCNSG++TI IA+KF CR ILGVDID                                 
Sbjct: 101 IGCNSGIMTIHIAKKFGCRSILGVDIDTSRIEDAHWHLRKFVRMQNSTKPSEKKSSSEGA 160

Query: 123 -------------------------------------------------LNSLECLSVAK 157
                                                             +++ CLSV K
Sbjct: 161 DGVHGSKEPSVSLSNGEAKADSAETKDLSQIVSFQKENFVQTRNLDDNRYDTILCLSVTK 220

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BLAST of Carg09833 vs. ExPASy Swiss-Prot
Match: Q9U2R0 (Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y17G7B.18 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-19
Identity = 60/203 (29.56%), Postives = 84/203 (41.38%), Query Frame = 0


Query: 4   EQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDI 63
           ++EK+ AE ++         + YGN+  YYG RL      D RL VF+K+WFE K  LDI
Sbjct: 97  KEEKSAAENRKQKTEYFNKKYRYGNFDRYYGIRLNPG-ESDKRLSVFQKDWFEHKQALDI 156

Query: 64  GCNSGMVTIQIARKFNCRRILGVDID---------------------------------- 123
           GCN+G +T+ IA+ F+ RRI+G+DID                                  
Sbjct: 157 GCNAGFLTLSIAKDFSPRRIIGIDIDEHLIGVARKNIRHYCDHETEVSGKFPASFGVQFG 216

Query: 124 ---------------------------------------PDLNSLECLSVAKWIHLNWGD 134
                                                  P+ + +  LS+ KWIHLNWGD
Sbjct: 217 TVSQRNEAPRSFSTKFPDNIWFKKENYVLESDEMLDMIQPEFDVILALSITKWIHLNWGD 276

[Close]

BLAST of Carg09833 vs. ExPASy Swiss-Prot
Match: Q9VNH1 (Probable RNA methyltransferase CG1239 OS=Drosophila melanogaster OX=7227 GN=CG1239 PE=2 SV=2)

HSP 1 Score: 87.0 bits (214), Expect = 2.0e-16
Identity = 57/173 (32.95%), Postives = 75/173 (43.35%), Query Frame = 0


Query: 4   EQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVF--KKEWFEGKDCL 63
           +  K    L   P + K   F YGNY+ YYG R+  K   D RL V   + + F  K  L
Sbjct: 55  QSPKKRLHLNGKPMQNKDLNFKYGNYKHYYGKRILNKDFHDIRLDVLGTQPDLFRNKQLL 114

Query: 64  DIGCNSGMVTIQIARKFNCRRILGVDID-------------------------------- 123
           DIGCNSG ++IQIARKF  + ++G+DID                                
Sbjct: 115 DIGCNSGHLSIQIARKFEVKSLVGLDIDRGLINDAQKTVSHLKRHATPGQGIPHIQFVHG 174

Query: 124 --------------PDLNSLECLSVAKWIHLNWGDDGLITLFYKIWRLLHPIG 129
                         P  + + CLSV KWIHLN+ D GL   F +++  L P G
Sbjct: 175 NYVLEDDVLLEIERPQFDVILCLSVTKWIHLNFCDSGLKQAFRRMYLQLRPGG 227

[Close]

BLAST of Carg09833 vs. ExPASy Swiss-Prot
Match: Q7L2J0 (7SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.0e-16
Identity = 57/212 (26.89%), Postives = 80/212 (37.74%), Query Frame = 0


Query: 18  RRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARK 77
           ++++  F YGNY  YYGYR      ED RL+V K EWF G+D LD+GCN G +T+ IA K
Sbjct: 407 KKQQRKFQYGNYCKYYGYR--NPSCEDGRLRVLKPEWFRGRDVLDLGCNVGHLTLSIACK 466

Query: 78  FNCRRILGVDID------------------------------------------------ 134
           +   R++G+DID                                                
Sbjct: 467 WGPSRMVGLDIDSRLIHSARQNIRHYLSEELRLPPQTLEGDPGAEGEEGTTTVRKRSCFP 526

[Close]

BLAST of Carg09833 vs. ExPASy Swiss-Prot
Match: Q8K3A9 (7SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 5.8e-16
Identity = 57/212 (26.89%), Postives = 80/212 (37.74%), Query Frame = 0


Query: 18  RRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARK 77
           ++++  F YGNY  YYGYR      ED RL+V K EWF+G+D LD+GCN G +T+ IA K
Sbjct: 384 KKQQLKFQYGNYCKYYGYR--NPSCEDVRLRVLKPEWFQGRDVLDLGCNVGHLTLSIACK 443

Query: 78  FNCRRILGVDIDPDL--------------------------------------------- 134
           +   R++G+DIDP L                                             
Sbjct: 444 WGPARMVGLDIDPRLIHSARQNIRHYLSEELRLQAQTSEGDPGTEGEEGTITVRKRSCFP 503

[Close]

BLAST of Carg09833 vs. ExPASy TrEMBL
Match: A0A6J1HBE1 (RNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461838 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 2.1e-69
Identity = 154/271 (56.83%), Postives = 154/271 (56.83%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDL-------------------NSLE----- 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPD                    N LE     
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ----------------------------------------------CLSVAKWIHLNWGD 157
                                                         CLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

[Close]

BLAST of Carg09833 vs. ExPASy TrEMBL
Match: A0A6J1JBF8 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.2e-68
Identity = 151/271 (55.72%), Postives = 153/271 (56.46%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEK EAELKQNPKRRKKHAFPYGNY+SYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKTEAELKQNPKRRKKHAFPYGNYRSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDL-------------------NSLE----- 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPD                    N LE     
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ----------------------------------------------CLSVAKWIHLNWGD 157
                                                         CLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

[Close]

BLAST of Carg09833 vs. ExPASy TrEMBL
Match: A0A6J1CV00 (RNA methyltransferase OS=Momordica charantia OX=3673 GN=LOC111014593 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 3.9e-55
Identity = 131/272 (48.16%), Postives = 140/272 (51.47%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEK EA+LK N K+RKK AFP+GNY+SYYGYR+GQ  VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKTEAQLKPNLKKRKKEAFPFGNYKSYYGYRIGQNLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPD--------------------LNSLE---- 120
           LDIGCN+G+VTIQIARKFNCR +LGVDID D                    +N LE    
Sbjct: 61  LDIGCNNGVVTIQIARKFNCRSMLGVDIDSDRIDDAYWNLKKATKLVVGQSVNGLELGAS 120

Query: 121 -----------------------------------------------CLSVAKWIHLNWG 157
                                                          CLSV KWIHLNWG
Sbjct: 121 GVPKVDTEDPTSTCSSKAQNLRKVISFRQRNFIFNDHPSDKHYDTILCLSVTKWIHLNWG 180

[Close]

BLAST of Carg09833 vs. ExPASy TrEMBL
Match: A0A5A7V9R8 (RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G001350 PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.1e-53
Identity = 124/272 (45.59%), Postives = 140/272 (51.47%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPD----------------------------- 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID D                             
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 ------------------------------------------LNSLECLSVAKWIHLNWG 157
                                                      +++ CLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

[Close]

BLAST of Carg09833 vs. ExPASy TrEMBL
Match: A0A5D3BG34 (RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold369G00080 PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.1e-53
Identity = 124/272 (45.59%), Postives = 140/272 (51.47%), Query Frame = 0


Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPD----------------------------- 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID D                             
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 ------------------------------------------LNSLECLSVAKWIHLNWG 157
                                                      +++ CLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

[Close]

BLAST of Carg09833 vs. TAIR 10
Match: AT5G51130.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 5.5e-38
Identity = 96/279 (34.41%), Postives = 120/279 (43.01%), Query Frame = 0


Query: 3   EEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLD 62
           ++Q+  +    Q+ K++ +  +P+GNY++YYGYR+     EDPRLKV KKEWFEGKDCLD
Sbjct: 41  KQQQGQQGNCNQSKKKKNQEVYPFGNYRNYYGYRISNDTDEDPRLKVLKKEWFEGKDCLD 100

Query: 63  IGCNSGMVTIQIARKFNCRRILGVDIDPD------------------------------- 122
           IGCNSG++TI IA+KF CR ILGVDID                                 
Sbjct: 101 IGCNSGIMTIHIAKKFGCRSILGVDIDTSRIEDAHWHLRKFVRMQNSTKPSEKKSSSEGA 160

Query: 123 -------------------------------------------------LNSLECLSVAK 157
                                                             +++ CLSV K
Sbjct: 161 DGVHGSKEPSVSLSNGEAKADSAETKDLSQIVSFQKENFVQTRNLDDNRYDTILCLSVTK 220

The following BLAST results are available for this feature:

BLAST of Carg09833 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023603.1	2.1e-87	100.00	putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6589938.1	4.4e-69	56.83	putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023516346.1	4.4e-69	56.83	probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022961298.1	4.4e-69	56.83	probable RNA methyltransferase At5g51130 [Cucurbita moschata]	[more]
XP_022987832.1	1.1e-67	55.72	probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg09833 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPC9	7.8e-37	34.41	Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9U2R0	2.5e-19	29.56	Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y1...	[more]
Q9VNH1	2.0e-16	32.95	Probable RNA methyltransferase CG1239 OS=Drosophila melanogaster OX=7227 GN=CG12...	[more]
Q7L2J0	2.0e-16	26.89	7SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 S...	[more]
Q8K3A9	5.8e-16	26.89	7SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 ...	[more]

BLAST of Carg09833 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBE1	2.1e-69	56.83	RNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461838 PE=3 SV=1	[more]
A0A6J1JBF8	5.2e-68	55.72	RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1	[more]
A0A6J1CV00	3.9e-55	48.16	RNA methyltransferase OS=Momordica charantia OX=3673 GN=LOC111014593 PE=3 SV=1	[more]
A0A5A7V9R8	2.1e-53	45.59	RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79...	[more]
A0A5D3BG34	2.1e-53	45.59	RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold36...	[more]

BLAST of Carg09833 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51130.1	5.5e-38	34.41	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 95..156 e-value: 6.8E-10 score: 40.8 coord: 22..94 e-value: 1.6E-27 score: 98.4
None	No IPR available	PANTHER	PTHR12315:SF0	7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME	coord: 4..92
None	No IPR available	PANTHER	PTHR12315:SF0	7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME	coord: 97..126
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 61..112 e-value: 0.00157356 score: 34.3279
IPR010675	RNA methyltransferase bin3, C-terminal	PFAM	PF06859	Bin3	coord: 97..129 e-value: 2.5E-10 score: 41.0
IPR039772	RNA methyltransferase Bin3-like	PANTHER	PTHR12315	BICOID-INTERACTING PROTEIN RELATED	coord: 4..92
IPR039772	RNA methyltransferase Bin3-like	PANTHER	PTHR12315	BICOID-INTERACTING PROTEIN RELATED	coord: 97..126
IPR024160	Bin3-type S-adenosyl-L-methionine binding domain	PROSITE	PS51515	BIN3_SAM	coord: 44..156 score: 23.850456
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 15..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09833-RA	Carg09833-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008168	methyltransferase activity