Carg09790 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09790
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding EF hand family protein
Location	Carg_Chr14: 2882898 .. 2883458 (-)
RNA-Seq Expression	Carg09790
Synteny	Carg09790

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACAACCCACAACCCACCATCAATCTGCAGCTCATCCCGTCAACGACAACAACGACGCCGCCGCTCGTTTTCATCGATCGGGCATCGATCTTCGCCGACACAATACACGACCTCGTCTCTCTAGCCTTCTCATTCTTTCAATCGCCCCCCAAAGATAAGGCTGCGCCACAAAGCAGAGCCTCACAATCAGAGAGCAAGGAAGATTGGTTGATGAGCAGAGAAGAAGTGAAATCTGTGATGGAAAAATTAGAGCTTTATTGCAACGAAGAAGAGGATGATGAAATTGATGAATGTTTTGGAAGGGATGAAGTAAGAGGCATGTTTGAAGAGAATGAACCGAGCTTGGAAGAGTTGAAGCAAACGTTCAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCACAGGAATTGCACACAGTTCTGGGCCTGCTTGCCTCTAATAATACCATATTCATCCATGACTGCAAATCAATGATAGCCAGATTTGATCGCAACAATGATCAAAAAATTGATTTCAATGAGTTTGTAAAGTTTATGGAGGTTGCCTTGTCCTAA

mRNA sequence

ATGCACAACCCACAACCCACCATCAATCTGCAGCTCATCCCGTCAACGACAACAACGACGCCGCCGCTCGTTTTCATCGATCGGGCATCGATCTTCGCCGACACAATACACGACCTCGTCTCTCTAGCCTTCTCATTCTTTCAATCGCCCCCCAAAGATAAGGCTGCGCCACAAAGCAGAGCCTCACAATCAGAGAGCAAGGAAGATTGGTTGATGAGCAGAGAAGAAGTGAAATCTGTGATGGAAAAATTAGAGCTTTATTGCAACGAAGAAGAGGATGATGAAATTGATGAATGTTTTGGAAGGGATGAAGTAAGAGGCATGTTTGAAGAGAATGAACCGAGCTTGGAAGAGTTGAAGCAAACGTTCAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCACAGGAATTGCACACAGTTCTGGGCCTGCTTGCCTCTAATAATACCATATTCATCCATGACTGCAAATCAATGATAGCCAGATTTGATCGCAACAATGATCAAAAAATTGATTTCAATGAGTTTGTAAAGTTTATGGAGGTTGCCTTGTCCTAA

Coding sequence (CDS)

ATGCACAACCCACAACCCACCATCAATCTGCAGCTCATCCCGTCAACGACAACAACGACGCCGCCGCTCGTTTTCATCGATCGGGCATCGATCTTCGCCGACACAATACACGACCTCGTCTCTCTAGCCTTCTCATTCTTTCAATCGCCCCCCAAAGATAAGGCTGCGCCACAAAGCAGAGCCTCACAATCAGAGAGCAAGGAAGATTGGTTGATGAGCAGAGAAGAAGTGAAATCTGTGATGGAAAAATTAGAGCTTTATTGCAACGAAGAAGAGGATGATGAAATTGATGAATGTTTTGGAAGGGATGAAGTAAGAGGCATGTTTGAAGAGAATGAACCGAGCTTGGAAGAGTTGAAGCAAACGTTCAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCACAGGAATTGCACACAGTTCTGGGCCTGCTTGCCTCTAATAATACCATATTCATCCATGACTGCAAATCAATGATAGCCAGATTTGATCGCAACAATGATCAAAAAATTGATTTCAATGAGTTTGTAAAGTTTATGGAGGTTGCCTTGTCCTAA

Protein sequence

MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSRASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKFMEVALS

Homology

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BLAST of Carg09790 vs. NCBI nr
Match: KAG7017701.1 (putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365.9 bits (938), Expect = 2.0e-97
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 187
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Carg09790 vs. NCBI nr
Match: XP_022934595.1 (probable calcium-binding protein CML30 [Cucurbita moschata])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 185/186 (99.46%), Postives = 185/186 (99.46%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFA TIHDLVSLAFSFFQSPPKDKAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFAGTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 187
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Carg09790 vs. NCBI nr
Match: KAG6580956.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 185/186 (99.46%), Postives = 185/186 (99.46%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSESK DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKGDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 187
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Carg09790 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359.0 bits (920), Expect = 2.5e-95
Identity = 181/186 (97.31%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDL+SL FSFFQSPPK+KAAPQSR
Sbjct: 11  MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 70

Query: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSE KEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 71  ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 130

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFD NNDQKIDFNEFVKF
Sbjct: 131 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF 190

Query: 181 MEVALS 187
           MEVALS
Sbjct: 191 MEVALS 196

[Close]

BLAST of Carg09790 vs. NCBI nr
Match: XP_022983385.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 176/187 (94.12%), Postives = 180/187 (96.26%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTT-TTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQS 60
           MHNPQPTINLQLIPSTTT   PPLVFIDRASIFADTIHDLVSLAFSFFQSPPK+KAAPQS
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKEKAAPQS 60

Query: 61  RASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL 120
           RASQSE KEDWLMSREEVKSVMEKLELYCNEE DDEIDECFGR+EVRGMFEENEPSLEEL
Sbjct: 61  RASQSEGKEDWLMSREEVKSVMEKLELYCNEEGDDEIDECFGREEVRGMFEENEPSLEEL 120

Query: 121 KQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVK 180
           KQTFNVFDRNRDGFIDA ELHTVLGLL SNNTIFIHDCKSMIARFDRNND+KIDFNEFVK
Sbjct: 121 KQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVK 180

Query: 181 FMEVALS 187
           FMEV+LS
Sbjct: 181 FMEVSLS 187

[Close]

BLAST of Carg09790 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.9e-22
Identity = 59/120 (49.17%), Postives = 76/120 (63.33%), Query Frame = 0


Query: 65  ESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFN 124
           +  +D  +SREE + VM  L L+ N   DD++ E +   EV  +FEE E SLEE+KQ F+
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYN---DDQLQEQYSAKEVSSLFEEKEASLEEVKQAFD 135

Query: 125 VFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKFMEVA 184
           VFD N+DGFIDA EL  VL +L      ++ +C  MI   D N D KIDFNEFVKFME +
Sbjct: 136 VFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of Carg09790 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.7e-18
Identity = 51/111 (45.95%), Postives = 71/111 (63.96%), Query Frame = 0


Query: 72  MSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFDRNRD 131
           + RE+V  VM+ L L   ++E++ + + +   EV  +FEE EPSLEE+KQ F+VFD NRD
Sbjct: 91  LCREDVGMVMKSLGL-STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRD 150

Query: 132 GFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKFME 183
           GFID  +L  VL +L       + +C+ MI  FD + D +IDF  FVKFME
Sbjct: 151 GFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of Carg09790 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.3e-10
Identity = 53/168 (31.55%), Postives = 84/168 (50.00%), Query Frame = 0


Query: 23  LVFIDRASIFADTIHDLVSLAFSFFQSPPKDK---AAPQSRASQSESKEDWLMSREEVKS 82
           ++  D +S F    H   S A+  F S   +K     P  +     ++ D   S EEVK+
Sbjct: 26  MIIQDFSSSFPFRFHLFFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKA 85

Query: 83  VMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL-KQTFNVFDRNRDGFIDAQE 142
           ++         ++ + + EC   +    + E+NE   +E+ K+ F +FD N+DGFID  E
Sbjct: 86  II---------DDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENE 145

Query: 143 LHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKFMEVALS 187
           L  VL LL  +    + +C+ M+  +D N D KIDF EFVK +E + S
Sbjct: 146 LKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of Carg09790 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-09
Identity = 54/207 (26.09%), Postives = 92/207 (44.44%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60
           ++N   +  L L+P    T  PL + D+      T+H     + S     P   +AP  R
Sbjct: 7   LYNILNSFLLSLVPKKLRTLFPLSWFDK------TLHKNSPPSPSTMLPSPSSSSAPTKR 66

Query: 61  ASQSESKE---------DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRD 120
              SE K          D  +++EE+   +E L +Y  +++        D   D C   D
Sbjct: 67  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 126

Query: 121 EVRGMF--------EENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIH 180
           E   ++         + E   E++K  FNVFD++ DGFI  +EL +V+  L       + 
Sbjct: 127 EFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 186

Query: 181 DCKSMIARFDRNNDQKIDFNEFVKFME 183
            CK MI + D + D ++++ EF++ M+
Sbjct: 187 GCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]

BLAST of Carg09790 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.3e-09
Identity = 38/125 (30.40%), Postives = 67/125 (53.60%), Query Frame = 0


Query: 69  DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRDEVRGMFE---ENEPSLE 128
           D  ++++E+   +E L +Y  +++        D   D     +E  G+++   E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 129 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEF 183
           ++++ FNVFD+NRDGFI  +EL +VL  L       + DCK MI++ D + D  ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

[Close]

BLAST of Carg09790 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.4e-97
Identity = 185/186 (99.46%), Postives = 185/186 (99.46%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFA TIHDLVSLAFSFFQSPPKDKAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFAGTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 187
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Carg09790 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 5.2e-91
Identity = 176/187 (94.12%), Postives = 180/187 (96.26%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTT-TTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQS 60
           MHNPQPTINLQLIPSTTT   PPLVFIDRASIFADTIHDLVSLAFSFFQSPPK+KAAPQS
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKEKAAPQS 60

Query: 61  RASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL 120
           RASQSE KEDWLMSREEVKSVMEKLELYCNEE DDEIDECFGR+EVRGMFEENEPSLEEL
Sbjct: 61  RASQSEGKEDWLMSREEVKSVMEKLELYCNEEGDDEIDECFGREEVRGMFEENEPSLEEL 120

Query: 121 KQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVK 180
           KQTFNVFDRNRDGFIDA ELHTVLGLL SNNTIFIHDCKSMIARFDRNND+KIDFNEFVK
Sbjct: 121 KQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVK 180

Query: 181 FMEVALS 187
           FMEV+LS
Sbjct: 181 FMEVSLS 187

[Close]

BLAST of Carg09790 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 6.2e-44
Identity = 105/189 (55.56%), Postives = 133/189 (70.37%), Query Frame = 0


Query: 6   PTINLQLIPSTTTTTPPLVFIDRASI------FADTIHDLVSLAFSFFQSPPKDKAAPQS 65
           P +NL+L P+       L+ +DR SI      F D IHDL+S  +S F SPPK+K +PQ 
Sbjct: 6   PIVNLKLAPT-------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKVSPQG 65

Query: 66  RASQSESKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLE 125
            + +SE+ +  +  +S+EEVK VMEKLE +C++EE +E     G +E+  +F E+EPSLE
Sbjct: 66  NSLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEE--SWLGGEEIGAIFYESEPSLE 125

Query: 126 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEF 185
           ELKQTFNVFD+NRDG IDA EL +VLGLL  N  +FIHDC  MIARFD N D KIDFNEF
Sbjct: 126 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 185

Query: 186 VKFMEVALS 187
           VKFME+ALS
Sbjct: 186 VKFMEIALS 185

[Close]

BLAST of Carg09790 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.4e-43
Identity = 105/189 (55.56%), Postives = 133/189 (70.37%), Query Frame = 0


Query: 6   PTINLQLIPSTTTTTPPLVFIDRASI------FADTIHDLVSLAFSFFQSPPKDKAAPQS 65
           P +NL+L  +       L+ +DR SI      F D IHDL+S  +S F SPPK+KA+PQ 
Sbjct: 6   PVVNLKLALT-------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQG 65

Query: 66  RASQSESKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLE 125
            + +SE+ +  +  +S+EEVK VMEKLE +C++EE +E     G +E+  +F E+EPSLE
Sbjct: 66  NSLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEE--SWLGGEEIGAIFYESEPSLE 125

Query: 126 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEF 185
           ELKQTFNVFD+NRDG IDA EL +VLGLL  N  +FIHDC  MIARFD N D KIDFNEF
Sbjct: 126 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 185

Query: 186 VKFMEVALS 187
           VKFME+ALS
Sbjct: 186 VKFMEIALS 185

[Close]

BLAST of Carg09790 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.6e-39
Identity = 117/196 (59.69%), Postives = 135/196 (68.88%), Query Frame = 0


Query: 9   NLQLIPSTTTTTPPLVFIDRAS------IFADTIHDLV-SLAF-SFFQSP-PKDKAAPQS 68
           ++QL PS      PLVF+D  S       FA  IH+L+ SL + S  QS   K+KA  QS
Sbjct: 11  SVQLDPS------PLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQS 70

Query: 69  R-ASQSESK------EDWLMSREEVKSVMEKLELYCNEEEDDEID--ECFGRDE-VRGMF 128
             +SQS+ K      ++  MSREEV+ VMEKLEL+  EE DD+ D  E    DE +RGMF
Sbjct: 71  NCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMF 130

Query: 129 EENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNND 186
           EENEPSLEELKQTFNVFDRN+DGFID  EL  VL LL SN  IFIHDCK+MIARFD NND
Sbjct: 131 EENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNND 190

[Close]

BLAST of Carg09790 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 106.7 bits (265), Expect = 2.1e-23
Identity = 59/120 (49.17%), Postives = 76/120 (63.33%), Query Frame = 0


Query: 65  ESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFN 124
           +  +D  +SREE + VM  L L+ N   DD++ E +   EV  +FEE E SLEE+KQ F+
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYN---DDQLQEQYSAKEVSSLFEEKEASLEEVKQAFD 135

Query: 125 VFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKFMEVA 184
           VFD N+DGFIDA EL  VL +L      ++ +C  MI   D N D KIDFNEFVKFME +
Sbjct: 136 VFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of Carg09790 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 91.7 bits (226), Expect = 6.9e-19
Identity = 51/111 (45.95%), Postives = 71/111 (63.96%), Query Frame = 0


Query: 72  MSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFDRNRD 131
           + RE+V  VM+ L L   ++E++ + + +   EV  +FEE EPSLEE+KQ F+VFD NRD
Sbjct: 91  LCREDVGMVMKSLGL-STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRD 150

Query: 132 GFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKFME 183
           GFID  +L  VL +L       + +C+ MI  FD + D +IDF  FVKFME
Sbjct: 151 GFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of Carg09790 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-11
Identity = 53/168 (31.55%), Postives = 84/168 (50.00%), Query Frame = 0


Query: 23  LVFIDRASIFADTIHDLVSLAFSFFQSPPKDK---AAPQSRASQSESKEDWLMSREEVKS 82
           ++  D +S F    H   S A+  F S   +K     P  +     ++ D   S EEVK+
Sbjct: 26  MIIQDFSSSFPFRFHLFFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKA 85

Query: 83  VMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL-KQTFNVFDRNRDGFIDAQE 142
           ++         ++ + + EC   +    + E+NE   +E+ K+ F +FD N+DGFID  E
Sbjct: 86  II---------DDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENE 145

Query: 143 LHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKFMEVALS 187
           L  VL LL  +    + +C+ M+  +D N D KIDF EFVK +E + S
Sbjct: 146 LKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of Carg09790 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 64.7 bits (156), Expect = 9.0e-11
Identity = 54/207 (26.09%), Postives = 92/207 (44.44%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60
           ++N   +  L L+P    T  PL + D+      T+H     + S     P   +AP  R
Sbjct: 7   LYNILNSFLLSLVPKKLRTLFPLSWFDK------TLHKNSPPSPSTMLPSPSSSSAPTKR 66

Query: 61  ASQSESKE---------DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRD 120
              SE K          D  +++EE+   +E L +Y  +++        D   D C   D
Sbjct: 67  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 126

Query: 121 EVRGMF--------EENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIH 180
           E   ++         + E   E++K  FNVFD++ DGFI  +EL +V+  L       + 
Sbjct: 127 EFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 186

Query: 181 DCKSMIARFDRNNDQKIDFNEFVKFME 183
            CK MI + D + D ++++ EF++ M+
Sbjct: 187 GCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]

BLAST of Carg09790 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 62.4 bits (150), Expect = 4.5e-10
Identity = 38/125 (30.40%), Postives = 67/125 (53.60%), Query Frame = 0


Query: 69  DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRDEVRGMFE---ENEPSLE 128
           D  ++++E+   +E L +Y  +++        D   D     +E  G+++   E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 129 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEF 183
           ++++ FNVFD+NRDGFI  +EL +VL  L       + DCK MI++ D + D  ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

The following BLAST results are available for this feature:

BLAST of Carg09790 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017701.1	2.0e-97	100.00	putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022934595.1	1.3e-96	99.46	probable calcium-binding protein CML30 [Cucurbita moschata]	[more]
KAG6580956.1	1.3e-96	99.46	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023528818.1	2.5e-95	97.31	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
XP_022983385.1	1.1e-90	94.12	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]

BLAST of Carg09790 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	2.9e-22	49.17	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	9.7e-18	45.95	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9SN89	3.3e-10	31.55	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
O22845	1.3e-09	26.09	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
Q9SRR7	6.3e-09	30.40	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]

BLAST of Carg09790 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F854	6.4e-97	99.46	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1J5Q6	5.2e-91	94.12	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1KQS0	6.2e-44	55.56	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]
A0A6J1EIK8	2.4e-43	55.56	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A1S3B8I1	1.6e-39	59.69	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]

BLAST of Carg09790 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	2.1e-23	49.17	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	6.9e-19	45.95	Calcium-binding EF-hand family protein	[more]
AT3G47480.1	2.4e-11	31.55	Calcium-binding EF-hand family protein	[more]
AT2G43290.1	9.0e-11	26.09	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	4.5e-10	30.40	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 156..184 e-value: 0.0011 score: 28.2 coord: 118..146 e-value: 3.1E-6 score: 36.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 117..182 e-value: 4.1E-9 score: 36.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 114..149 score: 15.015522
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 152..186 score: 10.691735
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 118..181 e-value: 2.93221E-14 score: 62.5653
None	No IPR available	GENE3D	1.10.238.10		coord: 99..183 e-value: 1.7E-18 score: 68.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..69
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 58..184
None	No IPR available	PANTHER	PTHR10891:SF805	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 58..184
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 165..177
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 127..139
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 68..181

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09790-RA	Carg09790-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding