Carg09691 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09691
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	AvrRpt-cleavage domain-containing protein
Location	Carg_Chr14: 3507137 .. 3508061 (+)
RNA-Seq Expression	Carg09691
Synteny	Carg09691

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCGGTAAGATTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTCCTTCTTTCATCTATTCCTCTTTTCTGTATGCATCTTTGCATCCCTGTTCGTTCATCGATCGAGTTTGATCGAACTGTTTGTATGGAGATTCTGCAACTTTTACCTGTAATGAATGAAAAATATGTAGCCATTGTATTGTAAGACATGGTAAATGTGTAAATCTATGTCATTTGCTCGTATCGATCGAGCGAGCAATGAGACGAGACAACCATTGAAACTTTCAGATATAAATCTATGATCTTACCTATGATGTTTTGTATCATTTTTTTGTGGTAAAACATTCAATATATAATCATGAATTACCTTCCCTTTGTTTCTCCCTGCCATGCTTGCTGCATTGTTTGATTCCCCCCAACAGCAGAAGGATTTCATTGCTTAAATAGAAATGCATGGCTAGAGAGATCTTGTAATAGTCTAAACCCACCGATAGTAGATATTGTCCAGTTTGGACTTTTCCTTTTGAAAACGCCTTTGTTAAGGAGAGGTTTCCACCATCCTCGTTCCAACTGAGGTGGGATCTCACAGATCTACACATAAAAATGGAACATTCTATGCAACAGAGAAGTGCATTTAAAGAACCGACAAACACGAGACTTCACAAGTATAGTTTGTCAATGAAGATCTGAATTCTGATATATGTATGTTTCTAATGATGTCTATAGCAGGATAGAGGTCGCCCCTTGCCGAAGTTCGGCGAGTGGGATGTCAACAACCCGGCCTCCGCCGAAGGCTTTACAGTAATATTTAGCAAAGCTAGAGACGAGAAGAAGTCAAATGAGGCTCCGGGGAACACAGCAGCAAAAAACAATGCATCCAGACCAGAAGAGAATTATCAATATTCCCCCATGGTAAGTAATCTCATTTACTAA

mRNA sequence

ATGGCTTCGGATAGAGGTCGCCCCTTGCCGAAGTTCGGCGAGTGGGATGTCAACAACCCGGCCTCCGCCGAAGGCTTTACAGTAATATTTAGCAAAGCTAGAGACGAGAAGAAGTCAAATGAGGCTCCGGGGAACACAGCAGCAAAAAACAATGCATCCAGACCAGAAGAGAATTATCAATATTCCCCCATGGTAAGTAATCTCATTTACTAA

Coding sequence (CDS)

ATGGCTTCGGATAGAGGTCGCCCCTTGCCGAAGTTCGGCGAGTGGGATGTCAACAACCCGGCCTCCGCCGAAGGCTTTACAGTAATATTTAGCAAAGCTAGAGACGAGAAGAAGTCAAATGAGGCTCCGGGGAACACAGCAGCAAAAAACAATGCATCCAGACCAGAAGAGAATTATCAATATTCCCCCATGGTAAGTAATCTCATTTACTAA

Protein sequence

MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQYSPMVSNLIY

Homology

[Close]

BLAST of Carg09691 vs. NCBI nr
Match: KAG7017799.1 (RPM1-interacting protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 144.4 bits (363), Expect = 3.6e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQ 60
          MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQ
Sbjct: 1  MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQ 60

Query: 61 YSPMVSNLIY 71
          YSPMVSNLIY
Sbjct: 61 YSPMVSNLIY 70

[Close]

BLAST of Carg09691 vs. NCBI nr
Match: KAG6581070.1 (Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 127.5 bits (319), Expect = 4.6e-26
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 4  DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQYSP 63
          DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQYSP
Sbjct: 27 DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQYSP 86

Query: 64 M 65
          M
Sbjct: 87 M 87

[Close]

BLAST of Carg09691 vs. NCBI nr
Match: KGN58221.1 (hypothetical protein Csa_017629 [Cucumis sativus])

HSP 1 Score: 125.2 bits (313), Expect = 2.3e-25
Identity = 60/64 (93.75%), Postives = 62/64 (96.88%), Query Frame = 0


Query: 1  MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQ 60
          MASD+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAK+N SR EENYQ
Sbjct: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60

Query: 61 YSPM 65
          YSPM
Sbjct: 61 YSPM 64

[Close]

BLAST of Carg09691 vs. NCBI nr
Match: XP_038883812.1 (protein NOI4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 123.2 bits (308), Expect = 8.6e-25
Identity = 58/61 (95.08%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 4  DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQYSP 63
          D+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNT AKNN SRPEENYQYSP
Sbjct: 30 DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTGAKNNTSRPEENYQYSP 89

Query: 64 M 65
          M
Sbjct: 90 M 90

[Close]

BLAST of Carg09691 vs. NCBI nr
Match: XP_038883820.1 (protein NOI4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 123.2 bits (308), Expect = 8.6e-25
Identity = 58/61 (95.08%), Postives = 59/61 (96.72%), Query Frame = 0


Query: 4  DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQYSP 63
          D+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNT AKNN SRPEENYQYSP
Sbjct: 12 DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTGAKNNTSRPEENYQYSP 71

Query: 64 M 65
          M
Sbjct: 72 M 72

[Close]

BLAST of Carg09691 vs. ExPASy Swiss-Prot
Match: O22633 (Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.2e-14
Identity = 35/43 (81.40%), Postives = 40/43 (93.02%), Query Frame = 0


Query: 3  SDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN 46
          SD+GRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+
Sbjct: 2  SDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGS 44

[Close]

BLAST of Carg09691 vs. ExPASy Swiss-Prot
Match: Q8GYN5 (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 7.9e-05
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 9   LPKFGEWDVNNPASAEGFTVIFSKARDEKKS--NEAPGNTAAKNNASRPEEN 59
           +PKFG+WD NNP+SA+G+T IF+K R+E+ S  N +  +    + +SR   N
Sbjct: 148 VPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPTHQSSRNPNN 199

[Close]

BLAST of Carg09691 vs. ExPASy TrEMBL
Match: A0A0A0LDP9 (AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G595220 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-25
Identity = 60/64 (93.75%), Postives = 62/64 (96.88%), Query Frame = 0


Query: 1  MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQ 60
          MASD+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAK+N SR EENYQ
Sbjct: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60

Query: 61 YSPM 65
          YSPM
Sbjct: 61 YSPM 64

[Close]

BLAST of Carg09691 vs. ExPASy TrEMBL
Match: A0A5D3E371 (RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold595G00620 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.7e-24
Identity = 57/63 (90.48%), Postives = 60/63 (95.24%), Query Frame = 0


Query: 2  ASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQY 61
          + D+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAK+N SR EENYQY
Sbjct: 19 SQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY 78

Query: 62 SPM 65
          SPM
Sbjct: 79 SPM 81

[Close]

BLAST of Carg09691 vs. ExPASy TrEMBL
Match: A0A5J5AK52 (AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_004861 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 6.0e-16
Identity = 46/66 (69.70%), Postives = 54/66 (81.82%), Query Frame = 0


Query: 2  ASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEA--PGNTAAKN-NASRPEEN 61
          + D+GRPLPKFGEWDVNNPASA+GFTVIF+KARD+KK+N A  PG  + KN N S+  EN
Sbjct: 3  SQDKGRPLPKFGEWDVNNPASADGFTVIFAKARDDKKTNGAAGPGGPSQKNDNTSKQNEN 62

Query: 62 YQYSPM 65
          YQY PM
Sbjct: 63 YQYPPM 68

[Close]

BLAST of Carg09691 vs. ExPASy TrEMBL
Match: B9SLS6 (NOI, putative OS=Ricinus communis OX=3988 GN=RCOM_0530960 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-15
Identity = 46/60 (76.67%), Postives = 50/60 (83.33%), Query Frame = 0


Query: 4  DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAA---KNNASRPEENYQ 61
          D+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSN A G  AA    NN  +P++NYQ
Sbjct: 5  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNAAGGAGAASQRNNNLHKPDDNYQ 64

[Close]

BLAST of Carg09691 vs. ExPASy TrEMBL
Match: A0A314URN2 (AvrRpt-cleavage domain-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_24221 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.3e-15
Identity = 46/63 (73.02%), Postives = 50/63 (79.37%), Query Frame = 0


Query: 1  MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQ 60
          MASD GRPLPKFGEWDVNNPASAEGFTVIF+KARD+K SN APG       +S+PE N Q
Sbjct: 1  MASDIGRPLPKFGEWDVNNPASAEGFTVIFTKARDQKMSNGAPGTGTP---SSKPEPNSQ 60

Query: 61 YSP 64
          Y P
Sbjct: 61 YPP 60

[Close]

BLAST of Carg09691 vs. TAIR 10
Match: AT5G63270.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.7e-15
Identity = 39/58 (67.24%), Postives = 46/58 (79.31%), Query Frame = 0


Query: 1  MASDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEEN 59
          MA ++G+PLPKFGEWDVNNPASAEGFTVIFSKA DEKK+ +A G  A  N+   P+ N
Sbjct: 1  MAENKGKPLPKFGEWDVNNPASAEGFTVIFSKASDEKKTKKASG--AGPNSLVSPQRN 56

[Close]

BLAST of Carg09691 vs. TAIR 10
Match: AT5G55850.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 78.2 bits (191), Expect = 3.0e-15
Identity = 35/43 (81.40%), Postives = 40/43 (93.02%), Query Frame = 0


Query: 3  SDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN 46
          SD+GRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+
Sbjct: 2  SDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGS 44

[Close]

BLAST of Carg09691 vs. TAIR 10
Match: AT5G55850.2 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 76.6 bits (187), Expect = 8.6e-15
Identity = 34/42 (80.95%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4  DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN 46
          D+GRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+
Sbjct: 19 DKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGS 60

[Close]

BLAST of Carg09691 vs. TAIR 10
Match: AT5G55850.3 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 76.6 bits (187), Expect = 8.6e-15
Identity = 34/42 (80.95%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4  DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN 46
          D+GRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+
Sbjct: 19 DKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGS 60

[Close]

BLAST of Carg09691 vs. TAIR 10
Match: AT2G04410.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 70.5 bits (171), Expect = 6.2e-13
Identity = 32/45 (71.11%), Postives = 41/45 (91.11%), Query Frame = 0


Query: 3  SDRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKK---SNEAPG 45
          +D+GRPLPKFGEWDVN+P+SAEGFTVIF+KAR+EKK    +++PG
Sbjct: 2  ADKGRPLPKFGEWDVNDPSSAEGFTVIFNKARNEKKGGGKSDSPG 46

The following BLAST results are available for this feature:

BLAST of Carg09691 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017799.1	3.6e-31	100.00	RPM1-interacting protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6581070.1	4.6e-26	100.00	Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KGN58221.1	2.3e-25	93.75	hypothetical protein Csa_017629 [Cucumis sativus]	[more]
XP_038883812.1	8.6e-25	95.08	protein NOI4-like isoform X1 [Benincasa hispida]	[more]
XP_038883820.1	8.6e-25	95.08	protein NOI4-like isoform X2 [Benincasa hispida]	[more]

BLAST of Carg09691 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22633	4.2e-14	81.40	Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1	[more]
Q8GYN5	7.9e-05	44.23	RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1	[more]

BLAST of Carg09691 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDP9	1.1e-25	93.75	AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G59...	[more]
A0A5D3E371	2.7e-24	90.48	RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A5J5AK52	6.0e-16	69.70	AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_0...	[more]
B9SLS6	1.3e-15	76.67	NOI, putative OS=Ricinus communis OX=3988 GN=RCOM_0530960 PE=4 SV=1	[more]
A0A314URN2	2.3e-15	73.02	AvrRpt-cleavage domain-containing protein OS=Prunus yedoensis var. nudiflora OX=...	[more]

BLAST of Carg09691 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G63270.1	1.7e-15	67.24	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.1	3.0e-15	81.40	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.2	8.6e-15	80.95	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.3	8.6e-15	80.95	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT2G04410.1	6.2e-13	71.11	RPM1-interacting protein 4 (RIN4) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008700	RIN4, pathogenic type III effector avirulence factor Avr cleavage site	PFAM	PF05627	AvrRpt-cleavage	coord: 4..39 e-value: 2.1E-23 score: 81.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..59
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..59
None	No IPR available	PANTHER	PTHR33159:SF69	RPM1-INTERACTING PROTEIN 4-LIKE	coord: 1..60
IPR040387	RPM1-interacting protein 4/NOI4	PANTHER	PTHR33159	RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN	coord: 1..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09691-RA	Carg09691-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005886	plasma membrane