Carg09647 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09647
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionmajor pollen allergen Ole e 1-like
LocationCarg_Chr14: 3701182 .. 3702073 (-)
RNA-Seq ExpressionCarg09647
SyntenyCarg09647
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCTGATTCTACTCTTAGTCTTAGCTTCCTTGGTTTCAAGTTCCCTTGTGTTGGGAATTCAGGCTGTTCATTCCCACCACTCAAGAATATTTTCTCAGATTACTGTTATGGGCCTTGTTTATTGTGACACCTGCTCCAACAATTCCTTCTCCAAGCACAGTTACTTCTTACCAGGTAATGCCCTAATTCATTTCTTCATACTATGTTGTTACTCGAGTAAGCTAAAGCTTAAGTTTAAGTCCTGAGTTCAACAAATTGAACCTGGACGAGACTTCAAATGGCAAAACTAGTGTCTAAGTGTTGCATAGTCTTCTTCAGCGTTTTGATTTTGAGTTTGAACTTTTTCGTTTTCGCAGCTTTGATGTTATGGTTGGAATTGAATGTCTACTTTGTTGATTTCTTGTGTGGTTTCTAACGAGATTTAAAGTGTTCTGTTGATTAGATTTTGAGTAACCTAATTTCTAAAATAGCATTGATTCAATTCAACCACTTATTCAAAGAATGATTGAGAAAATTTCTTTGTTTATAAACGAAACAAAAAATTAAACAGGTGCAGAAGTTCGAATCGACTGCAAATTAAAAGCAGAAACGCCGCGTACAGCGGAGCAAATAGCATTCTCAGTGAACAGAACAACAAACAAAAACGGAGTTTACAAATTGGAAATACCTTCGATGGACGGAATCCGATGTGCAGAGGATTCCTCAATGGCGTCGTTCTGCCAAGCGAGTTTGATGGGAAGTTCGTCGTCCGCTTGCAACGTTCCTGGATACAGAACCACATCGGGAGAAATTTCAATCAAATCGAGACAGTCAAACGTTTGCATTTACAGCTTAAATCCGCTGAATTACAGGCCATCAACAAGAGACGATATCTTGTGCGGGAATTAG

mRNA sequence

ATGAATCTGATTCTACTCTTAGTCTTAGCTTCCTTGGTTTCAAGTTCCCTTGTGTTGGGAATTCAGGCTGTTCATTCCCACCACTCAAGAATATTTTCTCAGATTACTGTTATGGGCCTTGTTTATTGTGACACCTGCTCCAACAATTCCTTCTCCAAGCACAGTTACTTCTTACCAGGTGCAGAAGTTCGAATCGACTGCAAATTAAAAGCAGAAACGCCGCGTACAGCGGAGCAAATAGCATTCTCAGTGAACAGAACAACAAACAAAAACGGAGTTTACAAATTGGAAATACCTTCGATGGACGGAATCCGATGTGCAGAGGATTCCTCAATGGCGTCGTTCTGCCAAGCGAGTTTGATGGGAAGTTCGTCGTCCGCTTGCAACGTTCCTGGATACAGAACCACATCGGGAGAAATTTCAATCAAATCGAGACAGTCAAACGTTTGCATTTACAGCTTAAATCCGCTGAATTACAGGCCATCAACAAGAGACGATATCTTGTGCGGGAATTAG

Coding sequence (CDS)

ATGAATCTGATTCTACTCTTAGTCTTAGCTTCCTTGGTTTCAAGTTCCCTTGTGTTGGGAATTCAGGCTGTTCATTCCCACCACTCAAGAATATTTTCTCAGATTACTGTTATGGGCCTTGTTTATTGTGACACCTGCTCCAACAATTCCTTCTCCAAGCACAGTTACTTCTTACCAGGTGCAGAAGTTCGAATCGACTGCAAATTAAAAGCAGAAACGCCGCGTACAGCGGAGCAAATAGCATTCTCAGTGAACAGAACAACAAACAAAAACGGAGTTTACAAATTGGAAATACCTTCGATGGACGGAATCCGATGTGCAGAGGATTCCTCAATGGCGTCGTTCTGCCAAGCGAGTTTGATGGGAAGTTCGTCGTCCGCTTGCAACGTTCCTGGATACAGAACCACATCGGGAGAAATTTCAATCAAATCGAGACAGTCAAACGTTTGCATTTACAGCTTAAATCCGCTGAATTACAGGCCATCAACAAGAGACGATATCTTGTGCGGGAATTAG

Protein sequence

MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPGAEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASLMGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN
Homology
BLAST of Carg09647 vs. NCBI nr
Match: KAG7017843.1 (hypothetical protein SDJN02_19709, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 335.5 bits (859), Expect = 2.7e-88
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG
Sbjct: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
           AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL
Sbjct: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN
Sbjct: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 171

BLAST of Carg09647 vs. NCBI nr
Match: XP_022934369.1 (major pollen allergen Ole e 1-like [Cucurbita moschata])

HSP 1 Score: 332.8 bits (852), Expect = 1.7e-87
Identity = 169/171 (98.83%), Postives = 170/171 (99.42%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG
Sbjct: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
           AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSM SFCQASL
Sbjct: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMVSFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           MGSSSSACNVPGY+TTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN
Sbjct: 121 MGSSSSACNVPGYKTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 171

BLAST of Carg09647 vs. NCBI nr
Match: XP_023527407.1 (major pollen allergen Ole e 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.2 bits (840), Expect = 4.3e-86
Identity = 167/171 (97.66%), Postives = 168/171 (98.25%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNLILL VLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFL G
Sbjct: 1   MNLILLFVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
           AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSM SFCQASL
Sbjct: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMVSFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           MGSSSSACNVPGY+TTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN
Sbjct: 121 MGSSSSACNVPGYKTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 171

BLAST of Carg09647 vs. NCBI nr
Match: XP_022983553.1 (major pollen allergen Ole e 1-like [Cucurbita maxima])

HSP 1 Score: 325.1 bits (832), Expect = 3.6e-85
Identity = 165/171 (96.49%), Postives = 167/171 (97.66%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNLILL VLASLVSSSLVLGIQA HSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFL G
Sbjct: 1   MNLILLFVLASLVSSSLVLGIQAAHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
           AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSM SFCQASL
Sbjct: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMVSFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           MGSSSSACNVPGY++TSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN
Sbjct: 121 MGSSSSACNVPGYKSTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 171

BLAST of Carg09647 vs. NCBI nr
Match: KAG6581110.1 (hypothetical protein SDJN03_21112, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.9 bits (829), Expect = 8.1e-85
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG
Sbjct: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
           AEVRIDCK KAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL
Sbjct: 61  AEVRIDCKFKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDI 168
           MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDI
Sbjct: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDI 167

BLAST of Carg09647 vs. ExPASy Swiss-Prot
Match: P19963 (Major pollen allergen Ole e 1 OS=Olea europaea OX=4146 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 35/128 (27.34%), Postives = 55/128 (42.97%), Query Frame = 0

Query: 33  SQITVMGLVYCDTCSNNSFSKHSYFLPGAEVRIDCKLKAETPRTAEQIAFSVNRTTNKNG 92
           SQ  + G VYCDTC     ++ S F+PGA +R+ CK K     T  ++ +     T   G
Sbjct: 9   SQFHIQGQVYCDTCRAGFITELSEFIPGASLRLQCKDKENGDVTFTEVGY-----TRAEG 68

Query: 93  VYKLEIPSMDGIRCAEDSSMASFCQASLMGSSSSACN-VPGYRTTSGEISIKSRQSNVCI 152
           +Y + +         E      FC+ +L+ S    CN +P        +  K    N   
Sbjct: 69  LYSMLV---------ERDHKNEFCEITLISSGRKDCNEIPTEGWAKPSLKFKLNTVNGTT 122

Query: 153 YSLNPLNY 160
            ++NPL +
Sbjct: 129 RTVNPLGF 122

BLAST of Carg09647 vs. ExPASy Swiss-Prot
Match: O82015 (Major pollen allergen Lig v 1 OS=Ligustrum vulgare OX=13597 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 3.9e-05
Identity = 35/128 (27.34%), Postives = 54/128 (42.19%), Query Frame = 0

Query: 33  SQITVMGLVYCDTCSNNSFSKHSYFLPGAEVRIDCKLKAETPRTAEQIAFSVNRTTNKNG 92
           SQ  + G VYCDTC     ++ S F+PGA VR+ CK       T  ++ +     T   G
Sbjct: 9   SQFYIQGQVYCDTCRARFITELSEFIPGAGVRLQCKDGENGKVTFTEVGY-----TKAEG 68

Query: 93  VYKLEIPSMDGIRCAEDSSMASFCQASLMGSSSSACN-VPGYRTTSGEISIKSRQSNVCI 152
           +Y + I         E      FC+ +L+ SS   C+ +P        +       N   
Sbjct: 69  LYNMLI---------ERDHKNEFCEITLISSSRKDCDEIPTEGWVKPSLKFVLNTVNGTT 122

Query: 153 YSLNPLNY 160
            ++NPL +
Sbjct: 129 RTINPLGF 122

BLAST of Carg09647 vs. ExPASy Swiss-Prot
Match: Q6NMJ2 (Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana OX=3702 GN=At4g18596 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 6.6e-05
Identity = 35/137 (25.55%), Postives = 60/137 (43.80%), Query Frame = 0

Query: 32  FSQITVMGLVYCDTCSNNSFSKHSYFLPGAEVRIDCKLKAETPRTAEQIAFSVNRTTNKN 91
           F +  + G VYCDTC     ++ S FL GA+V+++C+      RT   +  +    T+K 
Sbjct: 30  FDRFQIQGSVYCDTCRVQFVTRLSKFLEGAKVKLECR-----SRTNGTVTLTKEAVTDKT 89

Query: 92  GVYKLEIPSMDGIRCAEDSSMASFCQASLMGSSSSAC---NVPGYRTTSGEISIKSRQSN 151
           G Y++E+                 C+  L+ S  S C   +   Y   + +IS+ +    
Sbjct: 90  GSYRMEVTG---------DHEEEVCELVLVESPDSGCSDVSKEAYLRNAAKISLTANDGI 149

Query: 152 VCIYS--LNPLNYRPST 164
           V   +  +NPL +   T
Sbjct: 150 VSHETRIVNPLGFMVQT 152

BLAST of Carg09647 vs. ExPASy Swiss-Prot
Match: P33050 (Pollen-specific protein C13 OS=Zea mays OX=4577 GN=MGS1 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.6e-04
Identity = 31/124 (25.00%), Postives = 56/124 (45.16%), Query Frame = 0

Query: 37  VMGLVYCDTCSNNSFSKHSYFLPGAEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKL 96
           + G VYCDTC     +  + ++ GA+VR++CK          ++  +++  T+  G Y +
Sbjct: 37  IQGRVYCDTCRAGFVTNVTEYIAGAKVRLECKHFG-----TGKLERAIDGVTDATGTYTI 96

Query: 97  EIPSMDGIRCAEDSSMASFCQASLMGSSSSACN-VPGYRTTSGEISIKSRQSNVCIYSLN 156
           E+         +DS     CQ  L+ S    C+ V   R  +G +  ++   +  +   N
Sbjct: 97  EL---------KDSHEEDICQVVLVASPRKDCDEVQALRDRAGVLLTRNVGISDSLRPAN 146

Query: 157 PLNY 160
           PL Y
Sbjct: 157 PLGY 146

BLAST of Carg09647 vs. ExPASy TrEMBL
Match: A0A6J1F2D6 (major pollen allergen Ole e 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441559 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 8.5e-88
Identity = 169/171 (98.83%), Postives = 170/171 (99.42%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG
Sbjct: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
           AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSM SFCQASL
Sbjct: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMVSFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           MGSSSSACNVPGY+TTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN
Sbjct: 121 MGSSSSACNVPGYKTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 171

BLAST of Carg09647 vs. ExPASy TrEMBL
Match: A0A6J1J823 (major pollen allergen Ole e 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482122 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.8e-85
Identity = 165/171 (96.49%), Postives = 167/171 (97.66%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNLILL VLASLVSSSLVLGIQA HSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFL G
Sbjct: 1   MNLILLFVLASLVSSSLVLGIQAAHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
           AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSM SFCQASL
Sbjct: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMVSFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           MGSSSSACNVPGY++TSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN
Sbjct: 121 MGSSSSACNVPGYKSTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 171

BLAST of Carg09647 vs. ExPASy TrEMBL
Match: A0A1S3BPJ6 (major pollen allergen Ole e 1-like OS=Cucumis melo OX=3656 GN=LOC103491809 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.1e-76
Identity = 151/172 (87.79%), Postives = 160/172 (93.02%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSR-IFSQITVMGLVYCDTCSNNSFSKHSYFLP 60
           MNLILL VLASLV SS +  IQA HSH  R I+SQITVMGLVYCDTCSNN+FSKHSYFL 
Sbjct: 1   MNLILLFVLASLVPSSFLFEIQAAHSHPPRTIYSQITVMGLVYCDTCSNNTFSKHSYFLS 60

Query: 61  GAEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQAS 120
           GAEVRIDCKLKAETPRTAEQIAFSVNRTTNK GVYKLEIPS+DGIRCAEDS+M SFCQAS
Sbjct: 61  GAEVRIDCKLKAETPRTAEQIAFSVNRTTNKYGVYKLEIPSVDGIRCAEDSAMVSFCQAS 120

Query: 121 LMGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           LMGSSSS+CNVPGYR+TSGEISIKSRQSN+CIYSLNPLNY+PSTRDDILCGN
Sbjct: 121 LMGSSSSSCNVPGYRSTSGEISIKSRQSNICIYSLNPLNYKPSTRDDILCGN 172

BLAST of Carg09647 vs. ExPASy TrEMBL
Match: A0A0A0LDJ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G563290 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.8e-74
Identity = 148/172 (86.05%), Postives = 157/172 (91.28%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSR-IFSQITVMGLVYCDTCSNNSFSKHSYFLP 60
           MN ILL VLASLV S  +  I+A HSH  R  +SQITVMGLVYCDTCSNN+FSKHSYFL 
Sbjct: 1   MNHILLFVLASLVPSFFLFEIKAAHSHPPRTTYSQITVMGLVYCDTCSNNTFSKHSYFLS 60

Query: 61  GAEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQAS 120
           GAEVRIDCKLKAETPRTAEQIAFSVNRTTNK GVYKLEIPS+DGIRCAEDS+M SFCQAS
Sbjct: 61  GAEVRIDCKLKAETPRTAEQIAFSVNRTTNKYGVYKLEIPSVDGIRCAEDSAMVSFCQAS 120

Query: 121 LMGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           LMGSSSS+CNVPGYR+TSGEISIKSRQSN+CIYSLNPLNYRPSTRDDILCGN
Sbjct: 121 LMGSSSSSCNVPGYRSTSGEISIKSRQSNICIYSLNPLNYRPSTRDDILCGN 172

BLAST of Carg09647 vs. ExPASy TrEMBL
Match: A0A6J1ENF6 (pollen-specific protein-like At4g18596 OS=Cucurbita moschata OX=3662 GN=LOC111435137 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.0e-74
Identity = 143/171 (83.63%), Postives = 154/171 (90.06%), Query Frame = 0

Query: 1   MNLILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPG 60
           MNL LL +LA LV SSL+LGIQA HSH +R FSQITVMGLVYCDTCSNNSFSKHSYFL G
Sbjct: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASL 120
            EVRI CK KAETPRTAEQIAFSVNRTTNK GVY+LEIPS+DGIRCAEDS +ASFCQASL
Sbjct: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120

Query: 121 MGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 172
           +GS SS+CNVPGYRTTS EISIKSR++N+CIYSLNPLNYRPS RDD LCGN
Sbjct: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 171

BLAST of Carg09647 vs. TAIR 10
Match: AT5G41050.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.2e-41
Identity = 89/172 (51.74%), Postives = 120/172 (69.77%), Query Frame = 0

Query: 3   LILLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPGAE 62
           LI+LL+L  L+S    L   ++    ++   +ITVMGLVYCD CSNNSFS HSYF+PG E
Sbjct: 5   LIMLLLLLQLLS----LNSLSLKHSSAKPNGKITVMGLVYCDVCSNNSFSNHSYFIPGVE 64

Query: 63  VRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCA----EDSSMASFCQA 122
           VRI C+  + + RT E I FS NRTTN+ G+YKL+I S++G+ CA    +DS MAS CQA
Sbjct: 65  VRIICRFNSASSRTREMITFSANRTTNELGLYKLDITSLEGVACAAEAKKDSLMAS-CQA 124

Query: 123 SLMGSSSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCG 171
           SL+G S  +CNVPG+RTT+ ++  KS+ SN+C+Y    LN+RP  ++  LCG
Sbjct: 125 SLIGRSKDSCNVPGFRTTTEQVVFKSKISNLCVYGFTALNFRPLEKNLHLCG 171

BLAST of Carg09647 vs. TAIR 10
Match: AT5G13140.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 165.2 bits (417), Expect = 4.5e-41
Identity = 75/142 (52.82%), Postives = 108/142 (76.06%), Query Frame = 0

Query: 33  SQITVMGLVYCDTCSNNSFSKHSYFLPGAEVRIDCKLKAETPRTAEQIAFSVNRTTNKNG 92
           S+ITV+G+VYCDTCS N+FS+ SYFL G EV + C+ KA +P+TAE++  SVNRTTN++G
Sbjct: 37  SRITVVGVVYCDTCSINTFSRQSYFLQGVEVHVTCRFKASSPKTAEEVNISVNRTTNRSG 96

Query: 93  VYKLEIPSMDGIRCAEDSSMASFCQASLMGSSS---SACNVPGYRTTSGEISIKSRQSNV 152
           VYKLEIP +DGI C +  +++S C A ++ +SS     C++P ++T + E+SIKS+Q  V
Sbjct: 97  VYKLEIPHVDGIDCVDGIAISSQCSAKILKTSSDDNGGCSIPVFQTATNEVSIKSKQDRV 156

Query: 153 CIYSLNPLNYRPSTRDDILCGN 172
           CIYSL+ L+Y+P  ++  LCGN
Sbjct: 157 CIYSLSALSYKPPHKNTSLCGN 178

BLAST of Carg09647 vs. TAIR 10
Match: AT3G26960.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 163.7 bits (413), Expect = 1.3e-40
Identity = 88/170 (51.76%), Postives = 114/170 (67.06%), Query Frame = 0

Query: 5   LLLVLASLVSSSLVLGIQAVHSH-HSRIFSQITVMGLVYCDTCSNNSFSKHSYFLPGAEV 64
           L + L  L+   LV  + + HS    +  ++IT+MG VYCD CS NSFSKHSYF+ G EV
Sbjct: 6   LTMFLFILLQFLLVNSLSSKHSSPKPKPDAEITIMGFVYCDVCSKNSFSKHSYFMSGVEV 65

Query: 65  RIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASLMGS 124
           RI C+ KA +  T E I FS NRTTN+ G+YK+ I S+D   CA+  S+AS CQASL+G 
Sbjct: 66  RIVCRFKAASSTTTETITFSANRTTNEFGLYKVAISSLD---CADVDSLASSCQASLIGR 125

Query: 125 ---SSSACNVPGYRTTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCG 171
              S S+CN+PGYRTT+ ++  KS+QSN C+Y  N LN+RP  RD  LCG
Sbjct: 126 KNFSDSSCNIPGYRTTTDQVLFKSQQSNSCVYGFNALNFRPFKRDLALCG 172

BLAST of Carg09647 vs. TAIR 10
Match: AT5G15780.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.5e-07
Identity = 43/166 (25.90%), Postives = 76/166 (45.78%), Query Frame = 0

Query: 5   LLLVLASLVSSSLVLGIQAVHSHHSRIFSQITVMGLVYCDTCSNNSFSKH-SYFLPGAEV 64
           L++ L   ++  L  G Q V     +  S   V+G VYCDTC N +FSK  ++ + GA V
Sbjct: 13  LMIFLGISINGGLSQGQQHV---MKKTRSSAVVVGTVYCDTCFNGAFSKSPNHLISGALV 72

Query: 65  RIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMASFCQASLMGS 124
            ++C  +   P   +++       T+K G +K+++P       ++       C   L+ S
Sbjct: 73  AVECIDENSKPSFRQEV------KTDKRGEFKVKLP----FSVSKHVKKIKRCSVKLLSS 132

Query: 125 SSSACNVPGYRTTSGEISIKSRQ--SNVCIYSLNPLNYRPSTRDDI 168
           S   C++    T+S    +KS     N  ++S     +RP  + +I
Sbjct: 133 SQPYCSIASSATSSSLKRLKSNHHGENTRVFSAGFFTFRPENQPEI 165

BLAST of Carg09647 vs. TAIR 10
Match: AT5G45880.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 51.2 bits (121), Expect = 9.5e-07
Identity = 37/137 (27.01%), Postives = 60/137 (43.80%), Query Frame = 0

Query: 32  FSQITVMGLVYCDTCSNNSFSKHSYFLPGAEVRIDCKLKAETPRTAEQIAFSVNRTTNKN 91
           F +  + G VYCDTC     ++ S FL GA+V+++C+      RT   I  +    T+K 
Sbjct: 32  FDRFQIQGSVYCDTCRVQFVTRLSKFLEGAKVKLECR-----SRTNGTITLTKEAVTDKT 91

Query: 92  GVYKLEIPSMDGIRCAEDSSMASFCQASLMGSSSSAC---NVPGYRTTSGEISIKSRQSN 151
           G YK+E+                 C+  L+ S  S C   +   Y   + +IS+ +    
Sbjct: 92  GSYKMEVTG---------DHEEEVCELVLVQSPDSGCSDVSTEAYLRNAAKISLTANDGI 151

Query: 152 VCIYS--LNPLNYRPST 164
           V   +  +NPL +   T
Sbjct: 152 VSHETRIVNPLGFMVQT 154

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7017843.12.7e-88100.00hypothetical protein SDJN02_19709, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022934369.11.7e-8798.83major pollen allergen Ole e 1-like [Cucurbita moschata][more]
XP_023527407.14.3e-8697.66major pollen allergen Ole e 1-like [Cucurbita pepo subsp. pepo][more]
XP_022983553.13.6e-8596.49major pollen allergen Ole e 1-like [Cucurbita maxima][more]
KAG6581110.18.1e-8599.40hypothetical protein SDJN03_21112, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
P199631.6e-0627.34Major pollen allergen Ole e 1 OS=Olea europaea OX=4146 PE=1 SV=2[more]
O820153.9e-0527.34Major pollen allergen Lig v 1 OS=Ligustrum vulgare OX=13597 PE=1 SV=2[more]
Q6NMJ26.6e-0525.55Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana OX=3702 GN=At4g18... [more]
P330505.6e-0425.00Pollen-specific protein C13 OS=Zea mays OX=4577 GN=MGS1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2D68.5e-8898.83major pollen allergen Ole e 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441559... [more]
A0A6J1J8231.8e-8596.49major pollen allergen Ole e 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482122 P... [more]
A0A1S3BPJ61.1e-7687.79major pollen allergen Ole e 1-like OS=Cucumis melo OX=3656 GN=LOC103491809 PE=4 ... [more]
A0A0A0LDJ01.8e-7486.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G563290 PE=4 SV=1[more]
A0A6J1ENF67.0e-7483.63pollen-specific protein-like At4g18596 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
AT5G41050.11.2e-4151.74Pollen Ole e 1 allergen and extensin family protein [more]
AT5G13140.14.5e-4152.82Pollen Ole e 1 allergen and extensin family protein [more]
AT3G26960.11.3e-4051.76Pollen Ole e 1 allergen and extensin family protein [more]
AT5G15780.11.5e-0725.90Pollen Ole e 1 allergen and extensin family protein [more]
AT5G45880.19.5e-0727.01Pollen Ole e 1 allergen and extensin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01190Pollen_Ole_e_1coord: 37..135
e-value: 5.4E-23
score: 81.3
NoneNo IPR availablePANTHERPTHR46995OS09G0508200 PROTEINcoord: 3..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09647-RACarg09647-RAmRNA