Carg09574 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09574
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	MAPK kinase substrate protein At1g80180-like
Location	Carg_Chr17: 6919119 .. 6919475 (+)
RNA-Seq Expression	Carg09574
Synteny	Carg09574

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGTCTGCAGAGATCTGTCGTGTCATTCCGGCGGCAGGGATCTTCCGGTGTCGTTTGGGAAGACAAGTTCATCTCGGAGGAGCTCAACAAGGCGGCCCCAAGCGACGGTGAATCCAGCGCCACGCCCGAGGTGAAGACGGGAAGAAATATTCCGCCGATCAAGACGATTGACCGGACCCGATCAAACCGCGGGGGAGGACGGGGGTTCCGGAGCAAAGATATGGCGGCGCCGGCAGTGGAGCCCCCGTCACCAAGAGTCTCTGCTTGTGGCCTCTGCAGCGCTTTCGGGAAATCCGGCGCCAAGAAACAGAGAGCGGCGGCGGCGGCGGCTGGCAAGCGCCGATCACGGTAG

mRNA sequence

ATGGCTGGTCTGCAGAGATCTGTCGTGTCATTCCGGCGGCAGGGATCTTCCGGTGTCGTTTGGGAAGACAAGTTCATCTCGGAGGAGCTCAACAAGGCGGCCCCAAGCGACGGTGAATCCAGCGCCACGCCCGAGGTGAAGACGGGAAGAAATATTCCGCCGATCAAGACGATTGACCGGACCCGATCAAACCGCGGGGGAGGACGGGGGTTCCGGAGCAAAGATATGGCGGCGCCGGCAGTGGAGCCCCCGTCACCAAGAGTCTCTGCTTGTGGCCTCTGCAGCGCTTTCGGGAAATCCGGCGCCAAGAAACAGAGAGCGGCGGCGGCGGCGGCTGGCAAGCGCCGATCACGGTAG

Coding sequence (CDS)

ATGGCTGGTCTGCAGAGATCTGTCGTGTCATTCCGGCGGCAGGGATCTTCCGGTGTCGTTTGGGAAGACAAGTTCATCTCGGAGGAGCTCAACAAGGCGGCCCCAAGCGACGGTGAATCCAGCGCCACGCCCGAGGTGAAGACGGGAAGAAATATTCCGCCGATCAAGACGATTGACCGGACCCGATCAAACCGCGGGGGAGGACGGGGGTTCCGGAGCAAAGATATGGCGGCGCCGGCAGTGGAGCCCCCGTCACCAAGAGTCTCTGCTTGTGGCCTCTGCAGCGCTTTCGGGAAATCCGGCGCCAAGAAACAGAGAGCGGCGGCGGCGGCGGCTGGCAAGCGCCGATCACGGTAG

Protein sequence

MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDRTRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR

Homology

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BLAST of Carg09574 vs. NCBI nr
Match: KAG7014127.1 (MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 224.6 bits (571), Expect = 4.6e-55
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
           TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR
Sbjct: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 118

[Close]

BLAST of Carg09574 vs. NCBI nr
Match: KAG6575586.1 (MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219.2 bits (557), Expect = 1.9e-53
Identity = 117/118 (99.15%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
           TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR AAAAAGKRRSR
Sbjct: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR-AAAAAGKRRSR 117

[Close]

BLAST of Carg09574 vs. NCBI nr
Match: XP_023549291.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216.9 bits (551), Expect = 9.6e-53
Identity = 115/118 (97.46%), Postives = 117/118 (99.15%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVW+DKFISEELNKAAP+DGESSATPEVKTGRNIPPIKTIDR
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFISEELNKAAPNDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
           TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR AAAAAGKRRSR
Sbjct: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR-AAAAAGKRRSR 117

[Close]

BLAST of Carg09574 vs. NCBI nr
Match: XP_022991595.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita maxima])

HSP 1 Score: 214.5 bits (545), Expect = 4.8e-52
Identity = 114/118 (96.61%), Postives = 115/118 (97.46%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVW+DKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
            RSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR  AAAAGKRRSR
Sbjct: 61  ARSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR--AAAAGKRRSR 116

[Close]

BLAST of Carg09574 vs. NCBI nr
Match: XP_008461094.1 (PREDICTED: uncharacterized protein At1g15400-like [Cucumis melo] >KAA0058767.1 uncharacterized protein E6C27_scaffold339G002400 [Cucumis melo var. makuwa] >TYK10561.1 uncharacterized protein E5676_scaffold459G002140 [Cucumis melo var. makuwa])

HSP 1 Score: 175.3 bits (443), Expect = 3.2e-40
Identity = 93/118 (78.81%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVW+DKFI+EEL KA   +GESSA PE++T RNIPPIKT D 
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFIAEELRKAGKDEGESSAVPELRTARNIPPIKTNDP 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
           TRSN  GGRGFRSK+ A+PAVEPPSPRVSACGLCS FGKS  +KQR  AA AGKRRSR
Sbjct: 61  TRSNHAGGRGFRSKETASPAVEPPSPRVSACGLCSGFGKSNGQKQR--AALAGKRRSR 116

[Close]

BLAST of Carg09574 vs. ExPASy Swiss-Prot
Match: Q9SSC1 (MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g80180 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 8.9e-17
Identity = 62/139 (44.60%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSA----------TPEVK--- 60
           MAGLQRS +SFRRQGSSG+VW+D+ I+ EL++ A +D +             T EV+   
Sbjct: 1   MAGLQRSTISFRRQGSSGIVWDDRLIA-ELSQQAANDRKGETLQQDEQAKLITSEVQDQT 60

Query: 61  ----TGRNIPPIKT---IDRTRSNRGGG-RGFRSKDMAAPAVEPPSPRVSACGLCSAFGK 119
                G  + PI+T   ++R+RSN GG  R  R+    +PAV+PPSPR+SA G CSAFGK
Sbjct: 61  TKPIAGEGLKPIRTDGGMERSRSNGGGAIRHHRNTGRVSPAVDPPSPRLSAFGCCSAFGK 120

[Close]

BLAST of Carg09574 vs. ExPASy Swiss-Prot
Match: Q9XI29 (Uncharacterized protein At1g15400 OS=Arabidopsis thaliana OX=3702 GN=At1g15400 PE=1 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 7.5e-16
Identity = 60/141 (42.55%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAA---------------PSDGESSATPE 60
           M GLQRS +SFRRQGSSG+V++D+ I+ ELNK+                P    S     
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKTGRNIPPIKT-------IDRTRSNRGGG-RGFRSKDMAAPAVEPPSPRVSACGLCSAF 119
           +     + PIKT       I+R+RSN GG  R  R+    +PAV+PPSPR+S+CG CSAF
Sbjct: 61  IDEKDKLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSAF 120

[Close]

BLAST of Carg09574 vs. ExPASy TrEMBL
Match: A0A6J1JTD9 (MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC111488162 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.3e-52
Identity = 114/118 (96.61%), Postives = 115/118 (97.46%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVW+DKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
            RSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR  AAAAGKRRSR
Sbjct: 61  ARSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQR--AAAAGKRRSR 116

[Close]

BLAST of Carg09574 vs. ExPASy TrEMBL
Match: A0A5D3CFK2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002140 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.6e-40
Identity = 93/118 (78.81%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVW+DKFI+EEL KA   +GESSA PE++T RNIPPIKT D 
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFIAEELRKAGKDEGESSAVPELRTARNIPPIKTNDP 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
           TRSN  GGRGFRSK+ A+PAVEPPSPRVSACGLCS FGKS  +KQR  AA AGKRRSR
Sbjct: 61  TRSNHAGGRGFRSKETASPAVEPPSPRVSACGLCSGFGKSNGQKQR--AALAGKRRSR 116

[Close]

BLAST of Carg09574 vs. ExPASy TrEMBL
Match: A0A1S3CEE2 (uncharacterized protein At1g15400-like OS=Cucumis melo OX=3656 GN=LOC103499785 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.6e-40
Identity = 93/118 (78.81%), Postives = 101/118 (85.59%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDR 60
           MAGLQRSVVSFRRQGSSGVVW+DKFI+EEL KA   +GESSA PE++T RNIPPIKT D 
Sbjct: 1   MAGLQRSVVSFRRQGSSGVVWDDKFIAEELRKAGKDEGESSAVPELRTARNIPPIKTNDP 60

Query: 61  TRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
           TRSN  GGRGFRSK+ A+PAVEPPSPRVSACGLCS FGKS  +KQR  AA AGKRRSR
Sbjct: 61  TRSNHAGGRGFRSKETASPAVEPPSPRVSACGLCSGFGKSNGQKQR--AALAGKRRSR 116

[Close]

BLAST of Carg09574 vs. ExPASy TrEMBL
Match: A0A6J1KGD4 (MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC111494889 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.9e-38
Identity = 92/119 (77.31%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESS-ATPEVKTGRNIPPIKTID 60
           MAGLQRSVVSFRRQGSSG++W+DKFISEEL KA  +D ESS A  E+ TGRNIPPIKT+D
Sbjct: 1   MAGLQRSVVSFRRQGSSGLIWDDKFISEELIKAPQNDAESSTAAEELNTGRNIPPIKTVD 60

Query: 61  RTRSNRGGGRGFRSKDMAAPAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRRSR 119
           RTRSNRG  RGFRSKD AA AVEPPSPR+SACG+CSAF  SG KKQR    AAGKRRSR
Sbjct: 61  RTRSNRGEARGFRSKDTAAAAVEPPSPRISACGICSAFRNSGGKKQR--TTAAGKRRSR 117

[Close]

BLAST of Carg09574 vs. ExPASy TrEMBL
Match: A0A6J1HM35 (MAPK kinase substrate protein At1g80180-like OS=Cucurbita moschata OX=3662 GN=LOC111464222 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 6.8e-36
Identity = 90/122 (73.77%), Postives = 99/122 (81.15%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESS-ATPEVKTGRNIPPIKTID 60
           MAGLQRSVVSFRRQGSSG++W+DKFISEE+ KA  +D ESS A  E+ TGRNIPPIKT+D
Sbjct: 1   MAGLQRSVVSFRRQGSSGLIWDDKFISEEIIKAPQNDAESSTAAEELNTGRNIPPIKTVD 60

Query: 61  RTRSNRGGGRGFRSKDMA---APAVEPPSPRVSACGLCSAFGKSGAKKQRAAAAAAGKRR 119
           RTR NRG  RGFRSKD A   A AVEPPSPR+SACG+CSAF  SG KKQR    AAGKRR
Sbjct: 61  RTRLNRGEARGFRSKDTAAATATAVEPPSPRISACGICSAFRNSGGKKQR--TTAAGKRR 120

[Close]

BLAST of Carg09574 vs. TAIR 10
Match: AT1G80180.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15400.3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 87.8 bits (216), Expect = 6.3e-18
Identity = 62/139 (44.60%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSA----------TPEVK--- 60
           MAGLQRS +SFRRQGSSG+VW+D+ I+ EL++ A +D +             T EV+   
Sbjct: 1   MAGLQRSTISFRRQGSSGIVWDDRLIA-ELSQQAANDRKGETLQQDEQAKLITSEVQDQT 60

Query: 61  ----TGRNIPPIKT---IDRTRSNRGGG-RGFRSKDMAAPAVEPPSPRVSACGLCSAFGK 119
                G  + PI+T   ++R+RSN GG  R  R+    +PAV+PPSPR+SA G CSAFGK
Sbjct: 61  TKPIAGEGLKPIRTDGGMERSRSNGGGAIRHHRNTGRVSPAVDPPSPRLSAFGCCSAFGK 120

[Close]

BLAST of Carg09574 vs. TAIR 10
Match: AT1G15400.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 16 species: Archae - 0; Bacteria - 4; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 84.7 bits (208), Expect = 5.3e-17
Identity = 60/141 (42.55%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAA---------------PSDGESSATPE 60
           M GLQRS +SFRRQGSSG+V++D+ I+ ELNK+                P    S     
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKTGRNIPPIKT-------IDRTRSNRGGG-RGFRSKDMAAPAVEPPSPRVSACGLCSAF 119
           +     + PIKT       I+R+RSN GG  R  R+    +PAV+PPSPR+S+CG CSAF
Sbjct: 61  IDEKDKLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSAF 120

[Close]

BLAST of Carg09574 vs. TAIR 10
Match: AT1G15400.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 16 species: Archae - 0; Bacteria - 4; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 84.7 bits (208), Expect = 5.3e-17
Identity = 60/141 (42.55%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAA---------------PSDGESSATPE 60
           M GLQRS +SFRRQGSSG+V++D+ I+ ELNK+                P    S     
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKTGRNIPPIKT-------IDRTRSNRGGG-RGFRSKDMAAPAVEPPSPRVSACGLCSAF 119
           +     + PIKT       I+R+RSN GG  R  R+    +PAV+PPSPR+S+CG CSAF
Sbjct: 61  IDEKDKLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSAF 120

[Close]

BLAST of Carg09574 vs. TAIR 10
Match: AT1G15400.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 84 Blast hits to 84 proteins in 17 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 84.7 bits (208), Expect = 5.3e-17
Identity = 60/141 (42.55%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 1   MAGLQRSVVSFRRQGSSGVVWEDKFISEELNKAA---------------PSDGESSATPE 60
           M GLQRS +SFRRQGSSG+V++D+ I+ ELNK+                P    S     
Sbjct: 1   MEGLQRSTISFRRQGSSGIVFDDRLIA-ELNKSGNNEQKDESQRDEQPKPMSESSEQVKP 60

Query: 61  VKTGRNIPPIKT-------IDRTRSNRGGG-RGFRSKDMAAPAVEPPSPRVSACGLCSAF 119
           +     + PIKT       I+R+RSN GG  R  R+    +PAV+PPSPR+S+CG CSAF
Sbjct: 61  IDEKDKLRPIKTGGGAPGGIERSRSNGGGAQRHHRTTGRVSPAVDPPSPRISSCGCCSAF 120

[Close]

BLAST of Carg09574 vs. TAIR 10
Match: AT5G20100.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 82; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 73.9 bits (180), Expect = 9.4e-14
Identity = 45/116 (38.79%), Postives = 68/116 (58.62%), Query Frame = 0


Query: 4   LQRSVVSFRRQGSSGVVWEDKFISEELNKAAPSDGESSATPEVKTGRNIPPIKTIDRTRS 63
           LQRS  SFRRQGSSG++W D+F+S E+      + +     + + G       T+ R+ S
Sbjct: 3   LQRSTASFRRQGSSGLIWNDRFLSGEIRN---DERQEDRCNDHRDGSMAATTATVKRSAS 62

Query: 64  NRGGGRGFRSKDMAAPAVEPPSPRVSA--CGLCSAFGKSGAKKQRAAAAAAGKRRS 118
           + G G G R  ++ +PA++PPSP++SA  CG CS F  +G ++ R      G+RRS
Sbjct: 63  DGGCGHGGRLGEL-SPALDPPSPKISAVGCGFCSLFSSNGKRRSR------GRRRS 108

The following BLAST results are available for this feature:

BLAST of Carg09574 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014127.1	4.6e-55	100.00	MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
KAG6575586.1	1.9e-53	99.15	MAPK kinase substrate protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023549291.1	9.6e-53	97.46	MAPK kinase substrate protein At1g80180-like [Cucurbita pepo subsp. pepo]	[more]
XP_022991595.1	4.8e-52	96.61	MAPK kinase substrate protein At1g80180-like [Cucurbita maxima]	[more]
XP_008461094.1	3.2e-40	78.81	PREDICTED: uncharacterized protein At1g15400-like [Cucumis melo] >KAA0058767.1 u...	[more]

BLAST of Carg09574 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSC1	8.9e-17	44.60	MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g8...	[more]
Q9XI29	7.5e-16	42.55	Uncharacterized protein At1g15400 OS=Arabidopsis thaliana OX=3702 GN=At1g15400 P...	[more]

BLAST of Carg09574 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTD9	2.3e-52	96.61	MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A5D3CFK2	1.6e-40	78.81	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CEE2	1.6e-40	78.81	uncharacterized protein At1g15400-like OS=Cucumis melo OX=3656 GN=LOC103499785 P...	[more]
A0A6J1KGD4	1.9e-38	77.31	MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1HM35	6.8e-36	73.77	MAPK kinase substrate protein At1g80180-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of Carg09574 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80180.1	6.3e-18	44.60	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G15400.1	5.3e-17	42.55	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G15400.2	5.3e-17	42.55	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G15400.3	5.3e-17	42.55	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT5G20100.1	9.4e-14	38.79	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031421	Protein of unknown function DUF4666	PFAM	PF15697	DUF4666	coord: 1..105 e-value: 8.5E-31 score: 107.0
IPR031421	Protein of unknown function DUF4666	PANTHER	PTHR33730	OS05G0542732 PROTEIN-RELATED	coord: 1..115
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 99..118
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 30..87
None	No IPR available	PANTHER	PTHR33730:SF4	OS05G0542732 PROTEIN	coord: 1..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09574-RA	Carg09574-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity