Carg09445 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09445
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionThreonine synthase
LocationCarg_Chr17: 6181945 .. 6183555 (+)
RNA-Seq ExpressionCarg09445
SyntenyCarg09445
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCGCCTTCTTGAACTCCCATTCTCTCATCCTCAGATCTGATTCTCTCAAAACCCCTCAACAACCCAAGCTCCATTTCTCATCCTTCACCATCAAAGCCTCATCATCTTCGTCATCAGCTTCATCCGCTTCCAATTCCGGCGCTAATGTATCTCTCACAGGCAACAACATCGAACCTGTACCCTCAAAGCATCGCCGACCCGCTGATGAGAACATTCGCGACGAGGCTCGCCGCCATAACACCACTCATTCTCATCGGTTTTCCGCCAAATATGTCCCGTTTAATGCAGATCCATCGTCCGATGAATCGTATTCGCTCGATGAGATCATCTATCGGTCCCAATCTGGGGGCCTCCTCGATGTACAACATGATATGAACGCATTGAAGAATTACGATGGTAAATACTGGCGTAATCTATTCGATTCTCGTGTCGGGAAGACGACATGGCCGTATGGCTCGGGGGTCTGGTCGAAGAAAGAGTGGGTGTTGCCGGAGATTGATGGCGACGACATTGTTAGTGCGTTTGAAGGTAACTCCAATCTTTTTTGGGCCGAGCGTTTTGGCAAACAGTTTCTGGGCATGAGTGATTTGTGGGTTAAGCACTGTGGTAATAGTCATACGGGAAGTTTCAAGGATCTGGGAATGACAGTTTTGGTCAGTCAGGTCAATAGATTGCGTAAAATGCACAAGCCTGTCGTCGGAGTTGGTTGTGCTTCCACAGGGGACACGTCCGCCGCCTTATCGGCTTATTGCGCCGCCGCGGGGATTCCGTCTATTGTTTTTTTACCTGCTAACCGGATATCGCTTGCACAGTTGGTTCAGCCGATTGCAAATGGAGCTTTTGTTCTTAGTATCGACACTGATTTTGATGGTTGTATGCAGTTGATCAGGGAGGTTACGGCCGAATTACCCATTTATTTGGCAAATTCTCTGAACAGCTTGAGATTGGAAGGGCAAAAAACTGCCGCCATTGAAATTTTGCAACAGTTTGATTGGCAGGTTCCTGATTGGGTCATAGTTCCGGGAGGTAATCTTGGGAACATATATGCATTTTACAAAGGGTTTCACATGTGTAAAGAATTAGGACTTGTGGATAGGATTCCAAGGCTTGTCTGTGCTCAAGCTGCAAATGCCAATCCACTCTACTTGAACTACAAATCAGGTTGGGGTGAGTTCAAGGCTGTTAAAGCTAACACTACTTTTGCCTCGGCGATACAAATTGGTGATCCTGTCTCCATCGATAGAGCTGTTTATGCATTGAAGAATTCAAATGGGATTGTCGAGGAAGCAACTGAGGAGGAGCTGATGGATGCCATGGCTCAAGCGGACTCTACTGGCATGTTTATTTGTCCTCATACAGGGGTGGCTTTGACAGCATTGATCAAGCTTGTGAAGAGTGGAGTCATAGCTCCTACTGATCGAACCGTGGTCGTGAGCACGGCTCATGGCTTGAAGTTCACACAGGCCAAAATTGACTATCACTCAAATGAGATCAAGGATATGACTTGCCGTTTTGCAAACCCACCAGTGCAAGTTAATGCAGATTTTGGATCTGTCATGGATGTTTTGAAGAAATATCTGTTGAGTAAAAATCCGAAGTGTTAG

mRNA sequence

ATGGCGTCCGCCTTCTTGAACTCCCATTCTCTCATCCTCAGATCTGATTCTCTCAAAACCCCTCAACAACCCAAGCTCCATTTCTCATCCTTCACCATCAAAGCCTCATCATCTTCGTCATCAGCTTCATCCGCTTCCAATTCCGGCGCTAATGTATCTCTCACAGGCAACAACATCGAACCTGTACCCTCAAAGCATCGCCGACCCGCTGATGAGAACATTCGCGACGAGGCTCGCCGCCATAACACCACTCATTCTCATCGGTTTTCCGCCAAATATGTCCCGTTTAATGCAGATCCATCGTCCGATGAATCGTATTCGCTCGATGAGATCATCTATCGGTCCCAATCTGGGGGCCTCCTCGATGTACAACATGATATGAACGCATTGAAGAATTACGATGGTAAATACTGGCGTAATCTATTCGATTCTCGTGTCGGGAAGACGACATGGCCGTATGGCTCGGGGGTCTGGTCGAAGAAAGAGTGGGTGTTGCCGGAGATTGATGGCGACGACATTGTTAGTGCGTTTGAAGGTAACTCCAATCTTTTTTGGGCCGAGCGTTTTGGCAAACAGTTTCTGGGCATGAGTGATTTGTGGGTTAAGCACTGTGGTAATAGTCATACGGGAAGTTTCAAGGATCTGGGAATGACAGTTTTGGTCAGTCAGGTCAATAGATTGCGTAAAATGCACAAGCCTGTCGTCGGAGTTGGTTGTGCTTCCACAGGGGACACGTCCGCCGCCTTATCGGCTTATTGCGCCGCCGCGGGGATTCCGTCTATTGTTTTTTTACCTGCTAACCGGATATCGCTTGCACAGTTGGTTCAGCCGATTGCAAATGGAGCTTTTGTTCTTAGTATCGACACTGATTTTGATGGTTGTATGCAGTTGATCAGGGAGGTTACGGCCGAATTACCCATTTATTTGGCAAATTCTCTGAACAGCTTGAGATTGGAAGGGCAAAAAACTGCCGCCATTGAAATTTTGCAACAGTTTGATTGGCAGGTTCCTGATTGGGTCATAGTTCCGGGAGGTAATCTTGGGAACATATATGCATTTTACAAAGGGTTTCACATGTGTAAAGAATTAGGACTTGTGGATAGGATTCCAAGGCTTGTCTGTGCTCAAGCTGCAAATGCCAATCCACTCTACTTGAACTACAAATCAGGTTGGGGTGAGTTCAAGGCTGTTAAAGCTAACACTACTTTTGCCTCGGCGATACAAATTGGTGATCCTGTCTCCATCGATAGAGCTGTTTATGCATTGAAGAATTCAAATGGGATTGTCGAGGAAGCAACTGAGGAGGAGCTGATGGATGCCATGGCTCAAGCGGACTCTACTGGCATGTTTATTTGTCCTCATACAGGGGTGGCTTTGACAGCATTGATCAAGCTTGTGAAGAGTGGAGTCATAGCTCCTACTGATCGAACCGTGGTCGTGAGCACGGCTCATGGCTTGAAGTTCACACAGGCCAAAATTGACTATCACTCAAATGAGATCAAGGATATGACTTGCCGTTTTGCAAACCCACCAGTGCAAGTTAATGCAGATTTTGGATCTGTCATGGATGTTTTGAAGAAATATCTGTTGAGTAAAAATCCGAAGTGTTAG

Coding sequence (CDS)

ATGGCGTCCGCCTTCTTGAACTCCCATTCTCTCATCCTCAGATCTGATTCTCTCAAAACCCCTCAACAACCCAAGCTCCATTTCTCATCCTTCACCATCAAAGCCTCATCATCTTCGTCATCAGCTTCATCCGCTTCCAATTCCGGCGCTAATGTATCTCTCACAGGCAACAACATCGAACCTGTACCCTCAAAGCATCGCCGACCCGCTGATGAGAACATTCGCGACGAGGCTCGCCGCCATAACACCACTCATTCTCATCGGTTTTCCGCCAAATATGTCCCGTTTAATGCAGATCCATCGTCCGATGAATCGTATTCGCTCGATGAGATCATCTATCGGTCCCAATCTGGGGGCCTCCTCGATGTACAACATGATATGAACGCATTGAAGAATTACGATGGTAAATACTGGCGTAATCTATTCGATTCTCGTGTCGGGAAGACGACATGGCCGTATGGCTCGGGGGTCTGGTCGAAGAAAGAGTGGGTGTTGCCGGAGATTGATGGCGACGACATTGTTAGTGCGTTTGAAGGTAACTCCAATCTTTTTTGGGCCGAGCGTTTTGGCAAACAGTTTCTGGGCATGAGTGATTTGTGGGTTAAGCACTGTGGTAATAGTCATACGGGAAGTTTCAAGGATCTGGGAATGACAGTTTTGGTCAGTCAGGTCAATAGATTGCGTAAAATGCACAAGCCTGTCGTCGGAGTTGGTTGTGCTTCCACAGGGGACACGTCCGCCGCCTTATCGGCTTATTGCGCCGCCGCGGGGATTCCGTCTATTGTTTTTTTACCTGCTAACCGGATATCGCTTGCACAGTTGGTTCAGCCGATTGCAAATGGAGCTTTTGTTCTTAGTATCGACACTGATTTTGATGGTTGTATGCAGTTGATCAGGGAGGTTACGGCCGAATTACCCATTTATTTGGCAAATTCTCTGAACAGCTTGAGATTGGAAGGGCAAAAAACTGCCGCCATTGAAATTTTGCAACAGTTTGATTGGCAGGTTCCTGATTGGGTCATAGTTCCGGGAGGTAATCTTGGGAACATATATGCATTTTACAAAGGGTTTCACATGTGTAAAGAATTAGGACTTGTGGATAGGATTCCAAGGCTTGTCTGTGCTCAAGCTGCAAATGCCAATCCACTCTACTTGAACTACAAATCAGGTTGGGGTGAGTTCAAGGCTGTTAAAGCTAACACTACTTTTGCCTCGGCGATACAAATTGGTGATCCTGTCTCCATCGATAGAGCTGTTTATGCATTGAAGAATTCAAATGGGATTGTCGAGGAAGCAACTGAGGAGGAGCTGATGGATGCCATGGCTCAAGCGGACTCTACTGGCATGTTTATTTGTCCTCATACAGGGGTGGCTTTGACAGCATTGATCAAGCTTGTGAAGAGTGGAGTCATAGCTCCTACTGATCGAACCGTGGTCGTGAGCACGGCTCATGGCTTGAAGTTCACACAGGCCAAAATTGACTATCACTCAAATGAGATCAAGGATATGACTTGCCGTTTTGCAAACCCACCAGTGCAAGTTAATGCAGATTTTGGATCTGTCATGGATGTTTTGAAGAAATATCTGTTGAGTAAAAATCCGAAGTGTTAG

Protein sequence

MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIEPVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGLLDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGNSNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVVSTAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC
Homology
BLAST of Carg09445 vs. NCBI nr
Match: XP_023547948.1 (threonine synthase, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6575458.1 hypothetical protein SDJN03_26097, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014002.1 hypothetical protein SDJN02_24172, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 536/536 (100.00%), Postives = 536/536 (100.00%), Query Frame = 0

Query: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60
           MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE
Sbjct: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60

Query: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120
           PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL
Sbjct: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120

Query: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180
           LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN
Sbjct: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180

Query: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240
           SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA
Sbjct: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240

Query: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300
           STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE
Sbjct: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300

Query: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360
           VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC
Sbjct: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360

Query: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420
           KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY
Sbjct: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420

Query: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480
           ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV
Sbjct: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480

Query: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 537
           STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC
Sbjct: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 536

BLAST of Carg09445 vs. NCBI nr
Match: XP_022954062.1 (threonine synthase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1073.5 bits (2775), Expect = 4.1e-310
Identity = 535/536 (99.81%), Postives = 535/536 (99.81%), Query Frame = 0

Query: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60
           MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE
Sbjct: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60

Query: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120
           PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL
Sbjct: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120

Query: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180
           LDVQHDMN LKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN
Sbjct: 121 LDVQHDMNTLKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180

Query: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240
           SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA
Sbjct: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240

Query: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300
           STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE
Sbjct: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300

Query: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360
           VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC
Sbjct: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360

Query: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420
           KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY
Sbjct: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420

Query: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480
           ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV
Sbjct: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480

Query: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 537
           STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC
Sbjct: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 536

BLAST of Carg09445 vs. NCBI nr
Match: XP_022992224.1 (threonine synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.2e-309
Identity = 534/536 (99.63%), Postives = 536/536 (100.00%), Query Frame = 0

Query: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60
           MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE
Sbjct: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60

Query: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120
           PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL
Sbjct: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120

Query: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180
           LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN
Sbjct: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180

Query: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240
           SNLFWAERFGKQFLGM+DLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA
Sbjct: 181 SNLFWAERFGKQFLGMNDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240

Query: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300
           STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE
Sbjct: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300

Query: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360
           VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC
Sbjct: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360

Query: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420
           KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY
Sbjct: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420

Query: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480
           ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV
Sbjct: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480

Query: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 537
           STAHGLKFTQAKI+YHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC
Sbjct: 481 STAHGLKFTQAKIEYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 536

BLAST of Carg09445 vs. NCBI nr
Match: XP_022149379.1 (threonine synthase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 996.1 bits (2574), Expect = 1.2e-286
Identity = 501/532 (94.17%), Postives = 512/532 (96.24%), Query Frame = 0

Query: 1   MASAFLNSHSLILRSDSLKTP-QQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNI 60
           MASAF NSHSL+LRSDSL +P  QPK  F S +IKA   SSSASSASNSGA+VSLT  NI
Sbjct: 1   MASAFANSHSLLLRSDSLDSPLAQPKFRFFSSSIKA---SSSASSASNSGADVSLTNCNI 60

Query: 61  EPVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGG 120
           EPV SKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDE+IYRSQSGG
Sbjct: 61  EPVLSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEVIYRSQSGG 120

Query: 121 LLDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEG 180
           LLDVQHDM ALKNYDG+YWRNLFDSR+GKTTWPYGSGVWSKKEWVLPEID DDIVSAFEG
Sbjct: 121 LLDVQHDMAALKNYDGQYWRNLFDSRIGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEG 180

Query: 181 NSNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGC 240
           NSNLFWAERFGKQFLGM+DLWVKHCG SHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGC
Sbjct: 181 NSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGC 240

Query: 241 ASTGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIR 300
           ASTGDTSAALSAYCAAAGIPSIVFLPANRIS+AQLVQPIANGAFVLSIDTDFDGCMQLIR
Sbjct: 241 ASTGDTSAALSAYCAAAGIPSIVFLPANRISMAQLVQPIANGAFVLSIDTDFDGCMQLIR 300

Query: 301 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHM 360
           EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHM
Sbjct: 301 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHM 360

Query: 361 CKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAV 420
           CKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAV
Sbjct: 361 CKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAV 420

Query: 421 YALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVV 480
           YALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVAL+ALIKLVKSGVI PTDRTVV
Sbjct: 421 YALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALSALIKLVKSGVIGPTDRTVV 480

Query: 481 VSTAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLS 532
           VSTAHGLKFTQAKIDYHSNEIKDM CRFANPPVQV ADFGSVMDVLKKYLL+
Sbjct: 481 VSTAHGLKFTQAKIDYHSNEIKDMACRFANPPVQVKADFGSVMDVLKKYLLT 529

BLAST of Carg09445 vs. NCBI nr
Match: PON96203.1 (Threonine synthase-like [Trema orientale])

HSP 1 Score: 900.6 bits (2326), Expect = 6.6e-258
Identity = 455/515 (88.35%), Postives = 469/515 (91.07%), Query Frame = 0

Query: 20  TPQQP-KLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIEPVPSKHRRPADENIRDEA 79
           TP  P K H+   T     + S   S S       L         SK RRPADENIRDEA
Sbjct: 16  TPHHPTKPHYLKSTTTTIRAVSKPDSPSTPAPPPQLA--------SKPRRPADENIRDEA 75

Query: 80  RRHNTTHSHRFSAKYVPFNADPSS-DESYSLDEIIYRSQSGGLLDVQHDMNALKNYDGKY 139
           RRHNTTH+H FSAKYVPFNADPSS DESYSLDEI+YRSQSGGLLDVQHDM ALK YDGKY
Sbjct: 76  RRHNTTHTHHFSAKYVPFNADPSSADESYSLDEIVYRSQSGGLLDVQHDMEALKKYDGKY 135

Query: 140 WRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGNSNLFWAERFGKQFLGMS 199
           WR+LFDSRVGKTTWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQFLGM+
Sbjct: 136 WRDLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGMN 195

Query: 200 DLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCAAAG 259
           +LWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKM++PVVGVGCASTGDTSAALSAYCA+AG
Sbjct: 196 ELWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAALSAYCASAG 255

Query: 260 IPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLR 319
           IPSIVFLPANRIS+AQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLR
Sbjct: 256 IPSIVFLPANRISIAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLR 315

Query: 320 LEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQA 379
           LEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGF MCKELGLVD+IPRLVCAQA
Sbjct: 316 LEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFRMCKELGLVDKIPRLVCAQA 375

Query: 380 ANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEEL 439
           ANANPLYL+YKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEEL
Sbjct: 376 ANANPLYLHYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEEL 435

Query: 440 MDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVVSTAHGLKFTQAKIDYHS 499
           MDAMAQADSTGMFICPHTGVALTALIKL KSGVI PTDRTVVVSTAHGLKFTQ+KIDYHS
Sbjct: 436 MDAMAQADSTGMFICPHTGVALTALIKLRKSGVIKPTDRTVVVSTAHGLKFTQSKIDYHS 495

Query: 500 NEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSK 533
            EIKDM CRFANPPV V ADFGSVMDVLKKYLLSK
Sbjct: 496 KEIKDMACRFANPPVHVKADFGSVMDVLKKYLLSK 522

BLAST of Carg09445 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 2.1e-251
Identity = 439/526 (83.46%), Postives = 469/526 (89.16%), Query Frame = 0

Query: 10  SLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIEPVPSKHRRP 69
           S +LRS S  +P  P+LH          S+S  ++  +    +  T  N      KHRRP
Sbjct: 4   SCMLRS-SFISPGLPQLH--------HQSTSKPNNGIHFFTPIKATATNDAISQQKHRRP 63

Query: 70  ADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGLLDVQHDMNA 129
           ADENIR+EARRH    SH FSA+YVPFNA P+SDE YSLDEI+YRS+SGGLLDVQHDM+A
Sbjct: 64  ADENIREEARRH--CSSHNFSARYVPFNAGPNSDEWYSLDEIVYRSRSGGLLDVQHDMDA 123

Query: 130 LKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGNSNLFWAERF 189
           LK +DG+YWR+LFDSRVGKTTWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERF
Sbjct: 124 LKKFDGQYWRSLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERF 183

Query: 190 GKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAAL 249
           GKQFLGM+DLWVKHCG SHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAAL
Sbjct: 184 GKQFLGMTDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAAL 243

Query: 250 SAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYL 309
           SAYCA+AGIPSIVFLPAN+IS+AQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYL
Sbjct: 244 SAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYL 303

Query: 310 ANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRI 369
           ANSLNSLRLEGQKTAAIEILQQFDW+VP+WVIVPGGNLGNIYAFYKGF MCKELGLVDRI
Sbjct: 304 ANSLNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKELGLVDRI 363

Query: 370 PRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIV 429
           PRLVCAQAANANPLYL+YKSGW +FK VKANTTFASAIQIGDPVSIDRAV+AL+  NGIV
Sbjct: 364 PRLVCAQAANANPLYLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFALQQCNGIV 423

Query: 430 EEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVVSTAHGLKFT 489
           EEATEEELMDAMAQADSTGMFICPHTGVALTAL KL  SGVIAPTDRTVVVSTAHGLKFT
Sbjct: 424 EEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVSTAHGLKFT 483

Query: 490 QAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPK 536
           Q+KIDYHS EIKDM CRFANPPV+V ADFGSVMDVLK YLLS+N K
Sbjct: 484 QSKIDYHSKEIKDMECRFANPPVEVKADFGSVMDVLKSYLLSQNSK 518

BLAST of Carg09445 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 1.1e-239
Identity = 408/477 (85.53%), Postives = 439/477 (92.03%), Query Frame = 0

Query: 56  GNNIE-PVPSKHRRP--ADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEII 115
           GNNI+ P+ +  + P   ++NIRDEARR+ +   + FSAKYVPFNA P S ESYSLDEI+
Sbjct: 43  GNNIKAPIETAVKPPHRTEDNIRDEARRNRSNAVNPFSAKYVPFNAAPGSTESYSLDEIV 102

Query: 116 YRSQSGGLLDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDD 175
           YRS+SGGLLDV+HDM ALK +DG YWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DD
Sbjct: 103 YRSRSGGLLDVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDD 162

Query: 176 IVSAFEGNSNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHK 235
           IVSAFEGNSNLFWAERFGKQFLGM+DLWVKHCG SHTGSFKDLGMTVLVSQVNRLRKM +
Sbjct: 163 IVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKR 222

Query: 236 PVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFD 295
           PVVGVGCASTGDTSAALSAYCA+AGIPSIVFLPAN+IS+AQLVQPIANGAFVLSIDTDFD
Sbjct: 223 PVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFD 282

Query: 296 GCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYA 355
           GCM+LIRE+TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYA
Sbjct: 283 GCMKLIREITAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYA 342

Query: 356 FYKGFHMCKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDP 415
           FYKGF MC+ELGLVDRIPR+VCAQAANANPLYL+YKSGW +FK + A+TTFASAIQIGDP
Sbjct: 343 FYKGFKMCQELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDP 402

Query: 416 VSIDRAVYALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIA 475
           VSIDRAVYALK  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KL   GVIA
Sbjct: 403 VSIDRAVYALKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIA 462

Query: 476 PTDRTVVVSTAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYL 530
           PTDRTVVVSTAHGLKFTQ+KIDYHSN I DM CRF+NPPV V ADFG+VMDVLK YL
Sbjct: 463 PTDRTVVVSTAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Carg09445 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 2.6e-233
Identity = 412/509 (80.94%), Postives = 443/509 (87.03%), Query Frame = 0

Query: 27  HFSSFTIKASSSSSSASSASNSGANVSLTGNNIEP-VPSKHRRPADENIRDEARRHNTTH 86
           H +++    S +S    SA++     +     + P  P K RR  DENIRDEARR     
Sbjct: 8   HSATYFPSHSETSLKPHSAASFTVRCTSASPAVPPQTPQKPRRSPDENIRDEARR-RPHQ 67

Query: 87  SHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGLLDVQHDMNALKNYDGKYWRNLFDSR 146
               SA+YVPFNA PSS ESYSLDEI+YRSQSG LLDVQHD  ALK YDG++WRNLFDSR
Sbjct: 68  LQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFAALKRYDGEFWRNLFDSR 127

Query: 147 VGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGNSNLFWAERFGKQFLGMSDLWVKHCG 206
           VGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQ+L M+DLWVKHCG
Sbjct: 128 VGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQYLQMNDLWVKHCG 187

Query: 207 NSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLP 266
            SHTGSFKDLGM+VLVSQVNRLRKM+KPV+GVGCASTGDTSAALSAYCA+AGIPSIVFLP
Sbjct: 188 ISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAGIPSIVFLP 247

Query: 267 ANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAA 326
           A++IS+AQLVQPIANGAFVLSIDTDFDGCM LIREVTAELPIYLANSLNSLRLEGQKTAA
Sbjct: 248 ADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIYLANSLNSLRLEGQKTAA 307

Query: 327 IEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYL 386
           IEILQQF+WQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYL
Sbjct: 308 IEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYL 367

Query: 387 NYKSGWGE-FKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEELMDAMAQA 446
           +YKSG+ E F  +KANTTFASAIQIGDPVSIDRAVYALK SNGIVEEATEEELMDA A A
Sbjct: 368 HYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNGIVEEATEEELMDATALA 427

Query: 447 DSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVVSTAHGLKFTQAKIDYHSNEIKDMT 506
           DSTGMFICPHTGVALTAL+KL KSGVI   DRTVVVSTAHGLKFTQ+KIDYHS  IK+M 
Sbjct: 428 DSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLKFTQSKIDYHSKNIKEMA 487

Query: 507 CRFANPPVQVNADFGSVMDVLKKYLLSKN 534
           CR ANPPV+V A FGSVMDVLK+YL S +
Sbjct: 488 CRLANPPVKVKAKFGSVMDVLKEYLKSND 515

BLAST of Carg09445 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.4e-69
Identity = 161/427 (37.70%), Postives = 245/427 (57.38%), Query Frame = 0

Query: 104 ESYSLDEIIYRSQSGGLLDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEW 163
           ++Y +DEIIY  + GGLL++ +D   +K+        + + ++ K       GVW   E+
Sbjct: 10  KTYDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY 69

Query: 164 VLPEIDGDDIVSAFEGNSNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQ 223
            LP  D   IVS  EG + L+      K+ LG+ +L+VK+ G + TGSFKD GMTV V++
Sbjct: 70  -LPVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTR 129

Query: 224 VNRLRKMHKPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAF 283
            N L      V  VGCASTG+TSA+L+AY A +G   IV LP  +++L +L Q +  GA 
Sbjct: 130 ANEL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAK 189

Query: 284 VLSIDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVP 343
           V+ +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +WQVPD VIVP
Sbjct: 190 VIQVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVP 249

Query: 344 GGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTF 403
            GN GNI A +KGF   +  G++D +P++   QA  A P+   ++    +    K   T 
Sbjct: 250 VGNAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETI 309

Query: 404 ASAIQIGDPVSIDRAVYALKNSNGIVEEATEEELMDAM-AQADSTGMFICPHTGVALTAL 463
           A+AI+IG+PV+  +A+ A+ +S G  E  T+EE+++A    A   G+F+ P +  ++  L
Sbjct: 310 ATAIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGL 369

Query: 464 IKLVKSGVIAPTDRTVVVSTAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVM 523
            KL++ G+I   +R V ++T HGLK   A I            R +  P+++  D    M
Sbjct: 370 KKLLEEGIIDRDERIVCITTGHGLKDPDAAI------------RASEEPIKIECD----M 402

Query: 524 DVLKKYL 530
           +VLK+ L
Sbjct: 430 NVLKRIL 402

BLAST of Carg09445 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.6e-63
Identity = 154/391 (39.39%), Postives = 224/391 (57.29%), Query Frame = 0

Query: 106 YSLDEIIYR-SQSGGLLDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWV 165
           YS DE+IY  S+  GLLDV +D +++K           D    KT  P    VW   + +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVWKYAKLL 72

Query: 166 LPEIDGDDIVSAFEGNSNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQV 225
             E    + V+  EG + L+  +R  ++ +G+  L+VKH G + TGSFKD GMTV V++ 
Sbjct: 73  PVE---REPVTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVTKA 132

Query: 226 NRLRKMHKPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFV 285
             L      +  V CASTG+TSAAL+ Y A AGIP +V LPA +++L ++ Q + +GA V
Sbjct: 133 LEL-----GMNTVACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 286 LSIDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPG 345
           LSI  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D++VPD +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 346 GNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFA 405
           GN GNI A YKGF   K LG+ D +P++   QA  + P+    KSG       +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 406 SAIQIGDPVSIDRAVYALKNSNGIVEEATEEELMDAMAQ-ADSTGMFICPHTGVALTALI 465
           +AI+IG+PV+  +A+ A++ S G  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 466 KLVKSGVIAPTDRTVVVSTAHGLKFTQAKID 495
           KLV  GVI   +  V ++T H LK  Q  ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

BLAST of Carg09445 vs. ExPASy TrEMBL
Match: A0A6J1GPZ5 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111456440 PE=3 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 2.0e-310
Identity = 535/536 (99.81%), Postives = 535/536 (99.81%), Query Frame = 0

Query: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60
           MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE
Sbjct: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60

Query: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120
           PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL
Sbjct: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120

Query: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180
           LDVQHDMN LKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN
Sbjct: 121 LDVQHDMNTLKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180

Query: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240
           SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA
Sbjct: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240

Query: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300
           STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE
Sbjct: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300

Query: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360
           VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC
Sbjct: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360

Query: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420
           KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY
Sbjct: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420

Query: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480
           ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV
Sbjct: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480

Query: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 537
           STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC
Sbjct: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 536

BLAST of Carg09445 vs. ExPASy TrEMBL
Match: A0A6J1JWZ3 (Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111488612 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.0e-310
Identity = 534/536 (99.63%), Postives = 536/536 (100.00%), Query Frame = 0

Query: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60
           MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE
Sbjct: 1   MASAFLNSHSLILRSDSLKTPQQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIE 60

Query: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120
           PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL
Sbjct: 61  PVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGL 120

Query: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180
           LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN
Sbjct: 121 LDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGN 180

Query: 181 SNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240
           SNLFWAERFGKQFLGM+DLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA
Sbjct: 181 SNLFWAERFGKQFLGMNDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCA 240

Query: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300
           STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE
Sbjct: 241 STGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIRE 300

Query: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360
           VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC
Sbjct: 301 VTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMC 360

Query: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420
           KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY
Sbjct: 361 KELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVY 420

Query: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480
           ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV
Sbjct: 421 ALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVV 480

Query: 481 STAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 537
           STAHGLKFTQAKI+YHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC
Sbjct: 481 STAHGLKFTQAKIEYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSKNPKC 536

BLAST of Carg09445 vs. ExPASy TrEMBL
Match: A0A6J1D6W1 (Threonine synthase OS=Momordica charantia OX=3673 GN=LOC111017808 PE=3 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 5.6e-287
Identity = 501/532 (94.17%), Postives = 512/532 (96.24%), Query Frame = 0

Query: 1   MASAFLNSHSLILRSDSLKTP-QQPKLHFSSFTIKASSSSSSASSASNSGANVSLTGNNI 60
           MASAF NSHSL+LRSDSL +P  QPK  F S +IKA   SSSASSASNSGA+VSLT  NI
Sbjct: 1   MASAFANSHSLLLRSDSLDSPLAQPKFRFFSSSIKA---SSSASSASNSGADVSLTNCNI 60

Query: 61  EPVPSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGG 120
           EPV SKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDE+IYRSQSGG
Sbjct: 61  EPVLSKHRRPADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEVIYRSQSGG 120

Query: 121 LLDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEG 180
           LLDVQHDM ALKNYDG+YWRNLFDSR+GKTTWPYGSGVWSKKEWVLPEID DDIVSAFEG
Sbjct: 121 LLDVQHDMAALKNYDGQYWRNLFDSRIGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEG 180

Query: 181 NSNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGC 240
           NSNLFWAERFGKQFLGM+DLWVKHCG SHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGC
Sbjct: 181 NSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGC 240

Query: 241 ASTGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIR 300
           ASTGDTSAALSAYCAAAGIPSIVFLPANRIS+AQLVQPIANGAFVLSIDTDFDGCMQLIR
Sbjct: 241 ASTGDTSAALSAYCAAAGIPSIVFLPANRISMAQLVQPIANGAFVLSIDTDFDGCMQLIR 300

Query: 301 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHM 360
           EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHM
Sbjct: 301 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHM 360

Query: 361 CKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAV 420
           CKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAV
Sbjct: 361 CKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAV 420

Query: 421 YALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVV 480
           YALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVAL+ALIKLVKSGVI PTDRTVV
Sbjct: 421 YALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALSALIKLVKSGVIGPTDRTVV 480

Query: 481 VSTAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLS 532
           VSTAHGLKFTQAKIDYHSNEIKDM CRFANPPVQV ADFGSVMDVLKKYLL+
Sbjct: 481 VSTAHGLKFTQAKIDYHSNEIKDMACRFANPPVQVKADFGSVMDVLKKYLLT 529

BLAST of Carg09445 vs. ExPASy TrEMBL
Match: A0A2P5FEH0 (Threonine synthase OS=Trema orientale OX=63057 GN=TorRG33x02_081330 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.2e-258
Identity = 455/515 (88.35%), Postives = 469/515 (91.07%), Query Frame = 0

Query: 20  TPQQP-KLHFSSFTIKASSSSSSASSASNSGANVSLTGNNIEPVPSKHRRPADENIRDEA 79
           TP  P K H+   T     + S   S S       L         SK RRPADENIRDEA
Sbjct: 16  TPHHPTKPHYLKSTTTTIRAVSKPDSPSTPAPPPQLA--------SKPRRPADENIRDEA 75

Query: 80  RRHNTTHSHRFSAKYVPFNADPSS-DESYSLDEIIYRSQSGGLLDVQHDMNALKNYDGKY 139
           RRHNTTH+H FSAKYVPFNADPSS DESYSLDEI+YRSQSGGLLDVQHDM ALK YDGKY
Sbjct: 76  RRHNTTHTHHFSAKYVPFNADPSSADESYSLDEIVYRSQSGGLLDVQHDMEALKKYDGKY 135

Query: 140 WRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGNSNLFWAERFGKQFLGMS 199
           WR+LFDSRVGKTTWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQFLGM+
Sbjct: 136 WRDLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGMN 195

Query: 200 DLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCAAAG 259
           +LWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKM++PVVGVGCASTGDTSAALSAYCA+AG
Sbjct: 196 ELWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAALSAYCASAG 255

Query: 260 IPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLR 319
           IPSIVFLPANRIS+AQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLR
Sbjct: 256 IPSIVFLPANRISIAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLR 315

Query: 320 LEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQA 379
           LEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGF MCKELGLVD+IPRLVCAQA
Sbjct: 316 LEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFRMCKELGLVDKIPRLVCAQA 375

Query: 380 ANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEEL 439
           ANANPLYL+YKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEEL
Sbjct: 376 ANANPLYLHYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEEL 435

Query: 440 MDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVVSTAHGLKFTQAKIDYHS 499
           MDAMAQADSTGMFICPHTGVALTALIKL KSGVI PTDRTVVVSTAHGLKFTQ+KIDYHS
Sbjct: 436 MDAMAQADSTGMFICPHTGVALTALIKLRKSGVIKPTDRTVVVSTAHGLKFTQSKIDYHS 495

Query: 500 NEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSK 533
            EIKDM CRFANPPV V ADFGSVMDVLKKYLLSK
Sbjct: 496 KEIKDMACRFANPPVHVKADFGSVMDVLKKYLLSK 522

BLAST of Carg09445 vs. ExPASy TrEMBL
Match: A0A2P5BE61 (Threonine synthase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_246920 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 5.4e-258
Identity = 454/518 (87.64%), Postives = 476/518 (91.89%), Query Frame = 0

Query: 20  TPQQP-KLHF---SSFTIKASSSSSSASSASNSGANVSLTGNNIEPVPSKHRRPADENIR 79
           TP  P K H+   ++ TI+A S   S S+ +              P+ SK RRP+DENIR
Sbjct: 16  TPHHPTKPHYLKNTTTTIRAVSKPDSPSTPAPP-----------PPLVSKPRRPSDENIR 75

Query: 80  DEARRHNTTHSHRFSAKYVPFNADPSS-DESYSLDEIIYRSQSGGLLDVQHDMNALKNYD 139
           DEARRHNTTH+H FSAKYVPFNADPSS +ESYSLDEI+YRSQSGGLLDVQHDM ALK YD
Sbjct: 76  DEARRHNTTHTHHFSAKYVPFNADPSSANESYSLDEIVYRSQSGGLLDVQHDMEALKKYD 135

Query: 140 GKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGNSNLFWAERFGKQFL 199
           GKYWR++FDSRVGKTTWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQFL
Sbjct: 136 GKYWRDIFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFL 195

Query: 200 GMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCA 259
           GM++LWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKM++PVVGVGCASTGDTSAALSAYCA
Sbjct: 196 GMNELWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAALSAYCA 255

Query: 260 AAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLN 319
           +AGIPSIVFLPANRIS+AQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLN
Sbjct: 256 SAGIPSIVFLPANRISIAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLN 315

Query: 320 SLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVC 379
           SLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGF MCKELGLVD+IPRLVC
Sbjct: 316 SLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFRMCKELGLVDKIPRLVC 375

Query: 380 AQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATE 439
           AQAANANPLYL+YKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATE
Sbjct: 376 AQAANANPLYLHYKSGWGEFKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATE 435

Query: 440 EELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVVSTAHGLKFTQAKID 499
           EELMDAMAQADSTGMFICPHTGVALTALIKL KSGVI PTDRTVVVSTAHGLKFTQ+KID
Sbjct: 436 EELMDAMAQADSTGMFICPHTGVALTALIKLRKSGVIKPTDRTVVVSTAHGLKFTQSKID 495

Query: 500 YHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYLLSK 533
           YHS EIKDM CRFANPPV V ADFGSVMDVLKKYLLSK
Sbjct: 496 YHSKEIKDMACRFANPPVHVKADFGSVMDVLKKYLLSK 522

BLAST of Carg09445 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 830.5 bits (2144), Expect = 7.8e-241
Identity = 408/477 (85.53%), Postives = 439/477 (92.03%), Query Frame = 0

Query: 56  GNNIE-PVPSKHRRP--ADENIRDEARRHNTTHSHRFSAKYVPFNADPSSDESYSLDEII 115
           GNNI+ P+ +  + P   ++NIRDEARR+ +   + FSAKYVPFNA P S ESYSLDEI+
Sbjct: 43  GNNIKAPIETAVKPPHRTEDNIRDEARRNRSNAVNPFSAKYVPFNAAPGSTESYSLDEIV 102

Query: 116 YRSQSGGLLDVQHDMNALKNYDGKYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDGDD 175
           YRS+SGGLLDV+HDM ALK +DG YWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DD
Sbjct: 103 YRSRSGGLLDVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDD 162

Query: 176 IVSAFEGNSNLFWAERFGKQFLGMSDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMHK 235
           IVSAFEGNSNLFWAERFGKQFLGM+DLWVKHCG SHTGSFKDLGMTVLVSQVNRLRKM +
Sbjct: 163 IVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKR 222

Query: 236 PVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPANRISLAQLVQPIANGAFVLSIDTDFD 295
           PVVGVGCASTGDTSAALSAYCA+AGIPSIVFLPAN+IS+AQLVQPIANGAFVLSIDTDFD
Sbjct: 223 PVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFD 282

Query: 296 GCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYA 355
           GCM+LIRE+TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYA
Sbjct: 283 GCMKLIREITAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYA 342

Query: 356 FYKGFHMCKELGLVDRIPRLVCAQAANANPLYLNYKSGWGEFKAVKANTTFASAIQIGDP 415
           FYKGF MC+ELGLVDRIPR+VCAQAANANPLYL+YKSGW +FK + A+TTFASAIQIGDP
Sbjct: 343 FYKGFKMCQELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDP 402

Query: 416 VSIDRAVYALKNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLVKSGVIA 475
           VSIDRAVYALK  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KL   GVIA
Sbjct: 403 VSIDRAVYALKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIA 462

Query: 476 PTDRTVVVSTAHGLKFTQAKIDYHSNEIKDMTCRFANPPVQVNADFGSVMDVLKKYL 530
           PTDRTVVVSTAHGLKFTQ+KIDYHSN I DM CRF+NPPV V ADFG+VMDVLK YL
Sbjct: 463 PTDRTVVVSTAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Carg09445 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 809.3 bits (2089), Expect = 1.9e-234
Identity = 412/509 (80.94%), Postives = 443/509 (87.03%), Query Frame = 0

Query: 27  HFSSFTIKASSSSSSASSASNSGANVSLTGNNIEP-VPSKHRRPADENIRDEARRHNTTH 86
           H +++    S +S    SA++     +     + P  P K RR  DENIRDEARR     
Sbjct: 8   HSATYFPSHSETSLKPHSAASFTVRCTSASPAVPPQTPQKPRRSPDENIRDEARR-RPHQ 67

Query: 87  SHRFSAKYVPFNADPSSDESYSLDEIIYRSQSGGLLDVQHDMNALKNYDGKYWRNLFDSR 146
               SA+YVPFNA PSS ESYSLDEI+YRSQSG LLDVQHD  ALK YDG++WRNLFDSR
Sbjct: 68  LQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFAALKRYDGEFWRNLFDSR 127

Query: 147 VGKTTWPYGSGVWSKKEWVLPEIDGDDIVSAFEGNSNLFWAERFGKQFLGMSDLWVKHCG 206
           VGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQ+L M+DLWVKHCG
Sbjct: 128 VGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQYLQMNDLWVKHCG 187

Query: 207 NSHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLP 266
            SHTGSFKDLGM+VLVSQVNRLRKM+KPV+GVGCASTGDTSAALSAYCA+AGIPSIVFLP
Sbjct: 188 ISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAGIPSIVFLP 247

Query: 267 ANRISLAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAA 326
           A++IS+AQLVQPIANGAFVLSIDTDFDGCM LIREVTAELPIYLANSLNSLRLEGQKTAA
Sbjct: 248 ADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIYLANSLNSLRLEGQKTAA 307

Query: 327 IEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYL 386
           IEILQQF+WQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYL
Sbjct: 308 IEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYL 367

Query: 387 NYKSGWGE-FKAVKANTTFASAIQIGDPVSIDRAVYALKNSNGIVEEATEEELMDAMAQA 446
           +YKSG+ E F  +KANTTFASAIQIGDPVSIDRAVYALK SNGIVEEATEEELMDA A A
Sbjct: 368 HYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNGIVEEATEEELMDATALA 427

Query: 447 DSTGMFICPHTGVALTALIKLVKSGVIAPTDRTVVVSTAHGLKFTQAKIDYHSNEIKDMT 506
           DSTGMFICPHTGVALTAL+KL KSGVI   DRTVVVSTAHGLKFTQ+KIDYHS  IK+M 
Sbjct: 428 DSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLKFTQSKIDYHSKNIKEMA 487

Query: 507 CRFANPPVQVNADFGSVMDVLKKYLLSKN 534
           CR ANPPV+V A FGSVMDVLK+YL S +
Sbjct: 488 CRLANPPVKVKAKFGSVMDVLKEYLKSND 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023547948.10.0e+00100.00threonine synthase, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6575458.... [more]
XP_022954062.14.1e-31099.81threonine synthase, chloroplastic-like [Cucurbita moschata][more]
XP_022992224.11.2e-30999.63threonine synthase, chloroplastic-like [Cucurbita maxima][more]
XP_022149379.11.2e-28694.17threonine synthase, chloroplastic-like [Momordica charantia][more]
PON96203.16.6e-25888.35Threonine synthase-like [Trema orientale][more]
Match NameE-valueIdentityDescription
Q9MT282.1e-25183.46Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9S7B51.1e-23985.53Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ... [more]
Q9SSP52.6e-23380.94Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ... [more]
Q588601.4e-6937.70Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266... [more]
Q8TQD42.6e-6339.39Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /... [more]
Match NameE-valueIdentityDescription
A0A6J1GPZ52.0e-31099.81Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111456440 PE=3 SV=1[more]
A0A6J1JWZ36.0e-31099.63Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111488612 PE=3 SV=1[more]
A0A6J1D6W15.6e-28794.17Threonine synthase OS=Momordica charantia OX=3673 GN=LOC111017808 PE=3 SV=1[more]
A0A2P5FEH03.2e-25888.35Threonine synthase OS=Trema orientale OX=63057 GN=TorRG33x02_081330 PE=3 SV=1[more]
A0A2P5BE615.4e-25887.64Threonine synthase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_246920 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G29840.17.8e-24185.53Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT1G72810.11.9e-23480.94Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 288..534
e-value: 5.1E-73
score: 248.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 68..287
e-value: 2.9E-69
score: 235.8
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 73..528
IPR004450Threonine synthase-likeTIGRFAMTIGR00260TIGR00260coord: 164..487
e-value: 6.0E-62
score: 207.7
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 176..482
e-value: 3.4E-49
score: 167.9
NoneNo IPR availablePIRSRPIRSR038945-1PIRSR038945-1coord: 156..471
e-value: 2.2E-70
score: 235.2
NoneNo IPR availablePIRSRPIRSR038945-2PIRSR038945-2coord: 156..471
e-value: 2.2E-70
score: 235.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..72
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 48..533
NoneNo IPR availablePANTHERPTHR10314:SF176THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 48..533
NoneNo IPR availableCDDcd01563Thr-synth_1coord: 158..488
e-value: 8.9155E-148
score: 425.084
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 203..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09445-RACarg09445-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004795 threonine synthase activity