Carg09388 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09388
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	GDSL esterase/lipase EXL3-like
Location	Carg_Chr05: 1495141 .. 1496856 (-)
RNA-Seq Expression	Carg09388
Synteny	Carg09388

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTCCTCCTTCACTGAATTTCATGTTTTTTCTGTCTCTACTGCTCCTTTTGCATCTCCGACCTGCAATTGCTGCTATAACCATCCCTCCCGGCTACTCGGTTCCTGCCGTCTTCGTCTTCGGCGACTCGATCGTCGATACGGGCAACAACAACAATGTCATTACTCAAGCTAAGTGTAACTACCCGCCCTACGGTAGGGATTTCCCCAATGGACGCCCGACCGGCCGATTCTCCAACGGCAGGGTTCCCTCCGACCTAGTTGGTATAAACAATTTGCTTTTGAATATACGTATAACGTAATTGTAGGAACGGAATGGAGAGTTTGAATTTCATTTTTCGTTATTTTTAGCTGATGCTCTCGGTATCAAAGAACTCTTGCCACCGTATGCCGACCCAAATCTTCAACCCGAAGACTTAATTACCGGCGTTAACTTTGCTTCTGGTGGAGCGGGATTCGACCCTCTGACCTCTCAAACAGCGGTATATATCTATATATAAACTCATCTCACTTTTTCCCATTAAAATCACTAAATTACTTATCGTAATTTGAAAAGTTTATGGAAATTTCAACGCACTTTAATCCATTTAATCGACCACTTGTCACTATTGTAGTAACTTTTTGTCAATTTAACTGTAATTTACCTGATCAACCAAGGGTAAAATTAATTTAAGATAAATTCAAATGAAATTTTTTTGAAATTTCAGCCAGCAATTTCACTTGAGGATCAATTAGCAAATTTCAGAGCATATAAGAAGAAGCTAGAAGGGGTTGTAGGAGAAGAAAGAGCAAAGTTCATAATAGTCAATAGCCTTTACCTTGTGGTGGCTGGCAGCAATGACATAGCCAACACCTTTTATCTTGCAAGAATAAGACAACTTCACTTCACCATTGGTTCGTATACTGATTTCATGGCTCGATCCGCTTCCGCTTTCGTCAAGGTAAACCCTAAACCCGAATTCCGAGCTTAACCCACTGTTCCATTACACGTTTAGTTTCGTAAACTTTCACGTCTGTGTCTATTTAGTGCTTCAATTTTATAAACAGGGTTGTTAAACTTTCGTGTTTTTCTCCTGCAAAGGAACTGTACGCAGCTGGTGCACGAAGAATTGGGTTCTTCTCGACTCCACCGCTCGGATGCGTACCGTCGCAGAGAACTTTGGCGGGTGGGATTCAAAGGGCGTGTGTGAACGAGTATAATAATGCTGCAAAGCTTTTCAACGGGAAGTTGCATTCCACTCTAGATCACCTTCAAACTATACTCCCGGATAGTAGAGTGGTTTACGTGGATATTTACAACCCTTTACTTGATGTCATCCTAAACTACGTTAAGTATGGTATGTTCACCTTCAAACACAGTATACTGACACTGCCTTAATTCTAGGTCACTGTCATATCGTACCGTATTTCGACTCGGGACTAACTAATTGGCTAACGATTTTCAGGATTTGAAGTTGCAGATAGAGGGTGTTGTGGAAGCGGGTTAATTGAGGTGACGTTTTTGTGCAATAAGTTCGTGAAGACTTGCCCTGATAGCACCAAGTACGTGTTTTGGGATAGCTTTCATCCCACACAAACAACTTACACCCTCCTTGTTGCTCCTATCATCCAAAGATATATTTCTCGCTTTCTATAGTTCTAATTGATTTAACAGTAAACAACGTCACAATAATTTTCTCGAAATTTTAATAAACTCAAGATATTTATATTCTTCA

mRNA sequence

ATGCCTCCTCCTTCACTGAATTTCATGTTTTTTCTGTCTCTACTGCTCCTTTTGCATCTCCGACCTGCAATTGCTGCTATAACCATCCCTCCCGGCTACTCGGTTCCTGCCGTCTTCGTCTTCGGCGACTCGATCGTCGATACGGGCAACAACAACAATGTCATTACTCAAGCTAAGTGTAACTACCCGCCCTACGGTAGGGATTTCCCCAATGGACGCCCGACCGGCCGATTCTCCAACGGCAGGGTTCCCTCCGACCTAGTTGCTGATGCTCTCGGTATCAAAGAACTCTTGCCACCGTATGCCGACCCAAATCTTCAACCCGAAGACTTAATTACCGGCGTTAACTTTGCTTCTGGTGGAGCGGGATTCGACCCTCTGACCTCTCAAACAGCGCCAGCAATTTCACTTGAGGATCAATTAGCAAATTTCAGAGCATATAAGAAGAAGCTAGAAGGGGTTGTAGGAGAAGAAAGAGCAAAGTTCATAATAGTCAATAGCCTTTACCTTGTGGTGGCTGGCAGCAATGACATAGCCAACACCTTTTATCTTGCAAGAATAAGACAACTTCACTTCACCATTGGTTCGTATACTGATTTCATGGCTCGATCCGCTTCCGCTTTCGTCAAGGAACTGTACGCAGCTGGTGCACGAAGAATTGGGTTCTTCTCGACTCCACCGCTCGGATGCGTACCGTCGCAGAGAACTTTGGCGGGTGGGATTCAAAGGGCGTGTGTGAACGAGTATAATAATGCTGCAAAGCTTTTCAACGGGAAGTTGCATTCCACTCTAGATCACCTTCAAACTATACTCCCGGATAGTAGAGTGGTTTACGTGGATATTTACAACCCTTTACTTGATGTCATCCTAAACTACGTTAAGTATGGTATGTTCACCTTCAAACACAGTATACTGACACTGCCTTAATTCTAGGTCACTGTCATATCGTACCGTATTTCGACTCGGGACTAACTAATTGGCTAACGATTTTCAGGATTTGAAGTTGCAGATAGAGGGTGTTGTGGAAGCGGGTTAATTGAGGTGACGTTTTTGTGCAATAAGTTCGTGAAGACTTGCCCTGATAGCACCAAGTACGTGTTTTGGGATAGCTTTCATCCCACACAAACAACTTACACCCTCCTTGTTGCTCCTATCATCCAAAGATATATTTCTCGCTTTCTATAGTTCTAATTGATTTAACAGTAAACAACGTCACAATAATTTTCTCGAAATTTTAATAAACTCAAGATATTTATATTCTTCA

Coding sequence (CDS)

ATGCCTCCTCCTTCACTGAATTTCATGTTTTTTCTGTCTCTACTGCTCCTTTTGCATCTCCGACCTGCAATTGCTGCTATAACCATCCCTCCCGGCTACTCGGTTCCTGCCGTCTTCGTCTTCGGCGACTCGATCGTCGATACGGGCAACAACAACAATGTCATTACTCAAGCTAAGTGTAACTACCCGCCCTACGGTAGGGATTTCCCCAATGGACGCCCGACCGGCCGATTCTCCAACGGCAGGGTTCCCTCCGACCTAGTTGCTGATGCTCTCGGTATCAAAGAACTCTTGCCACCGTATGCCGACCCAAATCTTCAACCCGAAGACTTAATTACCGGCGTTAACTTTGCTTCTGGTGGAGCGGGATTCGACCCTCTGACCTCTCAAACAGCGCCAGCAATTTCACTTGAGGATCAATTAGCAAATTTCAGAGCATATAAGAAGAAGCTAGAAGGGGTTGTAGGAGAAGAAAGAGCAAAGTTCATAATAGTCAATAGCCTTTACCTTGTGGTGGCTGGCAGCAATGACATAGCCAACACCTTTTATCTTGCAAGAATAAGACAACTTCACTTCACCATTGGTTCGTATACTGATTTCATGGCTCGATCCGCTTCCGCTTTCGTCAAGGAACTGTACGCAGCTGGTGCACGAAGAATTGGGTTCTTCTCGACTCCACCGCTCGGATGCGTACCGTCGCAGAGAACTTTGGCGGGTGGGATTCAAAGGGCGTGTGTGAACGAGTATAATAATGCTGCAAAGCTTTTCAACGGGAAGTTGCATTCCACTCTAGATCACCTTCAAACTATACTCCCGGATAGTAGAGTGGTTTACGTGGATATTTACAACCCTTTACTTGATGTCATCCTAAACTACGTTAAGTATGGTATGTTCACCTTCAAACACAGTATACTGACACTGCCTTAA

Protein sequence

MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYGMFTFKHSILTLP

Homology

[Close]

BLAST of Carg09388 vs. NCBI nr
Match: KAG7029454.1 (GDSL esterase/lipase EXL3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.5 bits (1591), Expect = 6.4e-173
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60
           MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC
Sbjct: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60

Query: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120
           NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG
Sbjct: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120

Query: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180
           GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN
Sbjct: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180

Query: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240
           TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG
Sbjct: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240

Query: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYGMFTF 300
           IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYGMFTF
Sbjct: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYGMFTF 300

Query: 301 KHSILTLP 309
           KHSILTLP
Sbjct: 301 KHSILTLP 308

[Close]

BLAST of Carg09388 vs. NCBI nr
Match: KAG6598518.1 (GDSL esterase/lipase EXL3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 591.7 bits (1524), Expect = 3.8e-165
Identity = 295/296 (99.66%), Postives = 296/296 (100.00%), Query Frame = 0


Query: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60
           MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC
Sbjct: 49  MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 108

Query: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120
           NYPPYGRDFP+GRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG
Sbjct: 109 NYPPYGRDFPSGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 168

Query: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180
           GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN
Sbjct: 169 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 228

Query: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240
           TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG
Sbjct: 229 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 288

Query: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG
Sbjct: 289 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 344

[Close]

BLAST of Carg09388 vs. NCBI nr
Match: XP_022962225.1 (GDSL esterase/lipase EXL3-like [Cucurbita moschata])

HSP 1 Score: 585.1 bits (1507), Expect = 3.5e-163
Identity = 291/296 (98.31%), Postives = 293/296 (98.99%), Query Frame = 0


Query: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60
           MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC
Sbjct: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60

Query: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120
           NYPPYGRDFP+GRPTGRFSNGRVPSDLV DALGIKELLPPYADPNLQPEDLITGVNFASG
Sbjct: 61  NYPPYGRDFPSGRPTGRFSNGRVPSDLVVDALGIKELLPPYADPNLQPEDLITGVNFASG 120

Query: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180
           GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN
Sbjct: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180

Query: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240
           TFYLARIRQLHFTI SYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG
Sbjct: 181 TFYLARIRQLHFTISSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240

Query: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSR+VYVDIYNPLLDVILNY KYG
Sbjct: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRLVYVDIYNPLLDVILNYAKYG 296

[Close]

BLAST of Carg09388 vs. NCBI nr
Match: XP_023545211.1 (GDSL esterase/lipase EXL3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579.7 bits (1493), Expect = 1.5e-161
Identity = 289/296 (97.64%), Postives = 293/296 (98.99%), Query Frame = 0


Query: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60
           MPPPSLNFMFFLSLLLLLHLRPAI AITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC
Sbjct: 42  MPPPSLNFMFFLSLLLLLHLRPAIPAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 101

Query: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120
           NYPPYGRDF +GRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPE+LITGVNFASG
Sbjct: 102 NYPPYGRDFSDGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEELITGVNFASG 161

Query: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180
           GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN
Sbjct: 162 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 221

Query: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240
           TFYLARIRQLHFTI SYTDFMARSASAFV+ELYAAGARRIGFFSTPPLGCVPSQRTLAGG
Sbjct: 222 TFYLARIRQLHFTIDSYTDFMARSASAFVEELYAAGARRIGFFSTPPLGCVPSQRTLAGG 281

Query: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           IQRACVN+YNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG
Sbjct: 282 IQRACVNQYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 337

[Close]

BLAST of Carg09388 vs. NCBI nr
Match: XP_022997604.1 (GDSL esterase/lipase EXL3-like [Cucurbita maxima])

HSP 1 Score: 572.4 bits (1474), Expect = 2.4e-159
Identity = 286/296 (96.62%), Postives = 289/296 (97.64%), Query Frame = 0


Query: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60
           MPPPSLN MFFLSLLLLLHLRP IAAITIP GYSVPAVFVFGDSIVDTGNNNNVITQAKC
Sbjct: 42  MPPPSLNLMFFLSLLLLLHLRPTIAAITIPLGYSVPAVFVFGDSIVDTGNNNNVITQAKC 101

Query: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120
           NYPPYGRDFP G PTGRFSNGRVPSDLVADALGIK+LLPPYADPNLQPEDLITGVNFASG
Sbjct: 102 NYPPYGRDFPGGSPTGRFSNGRVPSDLVADALGIKKLLPPYADPNLQPEDLITGVNFASG 161

Query: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180
           GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEG+VGEERAKFIIVNSLYLVVAGSNDIAN
Sbjct: 162 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGLVGEERAKFIIVNSLYLVVAGSNDIAN 221

Query: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240
           TFYLARIRQLHFTI SYTDFMA+SASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG
Sbjct: 222 TFYLARIRQLHFTIDSYTDFMAQSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 281

Query: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           IQR CVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG
Sbjct: 282 IQRECVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 337

[Close]

BLAST of Carg09388 vs. ExPASy Swiss-Prot
Match: Q94CH6 (GDSL esterase/lipase EXL3 OS=Arabidopsis thaliana OX=3702 GN=EXL3 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.1e-85
Identity = 155/285 (54.39%), Postives = 202/285 (70.88%), Query Frame = 0


Query: 12  LSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGRDFPN 71
           + LL +L L   I A+ +PP   +PAV  FGDSIVDTG NNNV T  KC++ PYG +F +
Sbjct: 18  ICLLSVLFLTETITAVKLPPKLIIPAVIAFGDSIVDTGMNNNVKTVVKCDFLPYGINFQS 77

Query: 72  GRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQT 131
           G  TGRF +GRVP+DL+A+ LGIK ++P Y DPNL+ +DL+TGV+FASGG+G+DP+T + 
Sbjct: 78  GVATGRFCDGRVPADLLAEELGIKSIVPAYLDPNLKSKDLLTGVSFASGGSGYDPITPKL 137

Query: 132 APAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLARIRQLH 191
              ISLEDQL+ F  Y +K++ +VGE R  FI+ NSL+L+VAGS+DIANT+Y  R R   
Sbjct: 138 VAVISLEDQLSYFEEYIEKVKNIVGEARKDFIVANSLFLLVAGSDDIANTYYTLRARP-E 197

Query: 192 FTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVNEYNN 251
           + + SYT  M+ SAS FV +LY  G RR+  F  PP+GCVPSQRTL GGI R C + YN 
Sbjct: 198 YDVDSYTTLMSDSASEFVTKLYGYGVRRVAVFGAPPIGCVPSQRTLGGGILRDCADNYNE 257

Query: 252 AAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           AAKLFN KL   LD L+  LP  + +Y++IY+PL D+I N   YG
Sbjct: 258 AAKLFNSKLSPKLDSLRKTLPGIKPIYINIYDPLFDIIQNPANYG 301

[Close]

BLAST of Carg09388 vs. ExPASy Swiss-Prot
Match: Q94CH8 (GDSL esterase/lipase EXL1 OS=Arabidopsis thaliana OX=3702 GN=EXL1 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 8.3e-83
Identity = 158/290 (54.48%), Postives = 205/290 (70.69%), Query Frame = 0


Query: 8   FMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGR 67
           F     L+LL       A + IP   +VPAV VFGDSIVD GNN+++IT+A+C+Y PYG 
Sbjct: 23  FWCIFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMITEARCDYAPYGI 82

Query: 68  DFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPL 127
           DF  G  TGRFSNG+VP D+VA+ LGIK  +P Y +PNL+PE+L+TGV FASGGAG+ PL
Sbjct: 83  DFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGGAGYVPL 142

Query: 128 TSQTA-PAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLAR 187
           T++ A   I L  QL  F  Y +KL+ +VGE+R KFII NSL++V+ GSNDIAN F+   
Sbjct: 143 TTKIAVGGIPLPQQLIYFEEYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIANDFFTLP 202

Query: 188 IRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACV 247
             +LH+T+ S+T  MA +A +F + LY  GARRI  F  PP+GCVPSQRT+AGG  R CV
Sbjct: 203 PVRLHYTVASFTALMADNARSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGGPTRDCV 262

Query: 248 NEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
             +N+AAKLFN KL + +D L   L D  ++Y+DIY+PLLD+ILN  +YG
Sbjct: 263 ARFNDAAKLFNTKLSANIDVLSRTLQDPTIIYIDIYSPLLDLILNPHQYG 312

[Close]

BLAST of Carg09388 vs. ExPASy Swiss-Prot
Match: Q9FHW9 (GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana OX=3702 GN=At5g42170/At5g42160 PE=3 SV=2)

HSP 1 Score: 301.6 bits (771), Expect = 1.0e-80
Identity = 147/270 (54.44%), Postives = 192/270 (71.11%), Query Frame = 0


Query: 27  ITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGRDFPNGRPTGRFSNGRVPSD 86
           I +PP  ++P +  FGDSIVD+GNNN++ T  KCN+PPYG+DFP    TGRFS+GRVPSD
Sbjct: 40  IKLPPNVTIPGIITFGDSIVDSGNNNHLRTALKCNFPPYGKDFPGKIATGRFSDGRVPSD 99

Query: 87  LVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQTAPAISLEDQLANFRA 146
           +VA+ LGI E +P Y +P L+ EDL+ GVNFASGG+G+DPLT++    +SL DQL NF+ 
Sbjct: 100 IVAERLGIAETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLVKVVSLSDQLKNFQE 159

Query: 147 YKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLARIRQLHFTIGSYTDFMARSAS 206
           YK KL+ +VGEE+A F++ NSLYLVVA SNDIA+T+     R + +   SY D++A SAS
Sbjct: 160 YKNKLKVIVGEEKANFLVKNSLYLVVASSNDIAHTY---TARSIKYNKTSYADYLADSAS 219

Query: 207 AFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVNEYNNAAKLFNGKLHSTLDH 266
            FV  LY  GARRIG FS  P+GCVP+ RTL G ++R C  + N  A+ FN K+  TL+ 
Sbjct: 220 KFVSALYGLGARRIGVFSAVPVGCVPAARTLRGKLKRRCSEKLNEVARNFNAKISPTLEA 279

Query: 267 LQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           L   LPDSRVV +D+ + L D+I N   YG
Sbjct: 280 LGKELPDSRVVLIDVCDTLNDMIENPKNYG 306

[Close]

BLAST of Carg09388 vs. ExPASy Swiss-Prot
Match: Q9LH73 (GDSL esterase/lipase At3g14820 OS=Arabidopsis thaliana OX=3702 GN=At3g14820 PE=3 SV=2)

HSP 1 Score: 283.1 bits (723), Expect = 3.7e-75
Identity = 135/263 (51.33%), Postives = 188/263 (71.48%), Query Frame = 0


Query: 34  SVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGRDFPNGRPTGRFSNGRVPSDLVADALG 93
           ++PA+ VFGDSI+DTGNNN++ T  K N+PPYGRDFP   PTGRFS+G+VPSD++A++LG
Sbjct: 29  TIPALIVFGDSIMDTGNNNDIPTLLKSNFPPYGRDFPGAIPTGRFSDGKVPSDIIAESLG 88

Query: 94  IKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQTAPAISLEDQLANFRAYKKKLEG 153
           I + LPPY   NL+P DL+ GV FASGG+G+DPLTS     +S+ DQL  F+ Y  K++ 
Sbjct: 89  IAKTLPPYLGSNLKPHDLLKGVIFASGGSGYDPLTSTLLSVVSMSDQLKYFQEYLAKIKQ 148

Query: 154 VVGEERAKFIIVNSLYLVVAGSNDIANTFYLARIRQLHFTIGSYTDFMARSASAFVKELY 213
             GEE+ KFI+  S++LVV+ SND+A T++   +R + +   SY +++   AS F+KEL 
Sbjct: 149 HFGEEKVKFILEKSVFLVVSSSNDLAETYW---VRSVEYDRNSYAEYLVELASEFIKELS 208

Query: 214 AAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVNEYNNAAKLFNGKLHSTLDHLQTILPD 273
             GA+ IG FS  P+GC+P+QRTL GG +R C  + NN A  FN KL S+LD L+  LP 
Sbjct: 209 ELGAKNIGLFSGVPVGCLPAQRTLFGGFERKCYEKLNNMALHFNSKLSSSLDTLKKELP- 268

Query: 274 SRVVYVDIYNPLLDVILNYVKYG 297
           SR++++D+Y+ LLD+I N   YG
Sbjct: 269 SRLIFIDVYDTLLDIIKNPTNYG 287

[Close]

BLAST of Carg09388 vs. ExPASy Swiss-Prot
Match: Q94CH7 (GDSL esterase/lipase EXL2 OS=Arabidopsis thaliana OX=3702 GN=EXL2 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 6.4e-75
Identity = 146/296 (49.32%), Postives = 194/296 (65.54%), Query Frame = 0


Query: 15  LLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQ-AKCNYPPYGRDFPNGR 74
           L+LL      A +  PP  + PA+ VFGDSIVD GNN++++T  A+CNYPPYG DF  G 
Sbjct: 25  LVLLCKTSTNALVKQPPNETTPAIIVFGDSIVDAGNNDDIMTTLARCNYPPYGIDFDGGI 84

Query: 75  PTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQTAP 134
           PTGRF NG+V +D +A   GIK  +P Y +PNL+PEDL+TGV FASGGAG+ P T+Q + 
Sbjct: 85  PTGRFCNGKVATDFIAGKFGIKPSIPAYRNPNLKPEDLLTGVTFASGGAGYVPFTTQLST 144

Query: 135 -------------AISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 194
                         I+L  QL  F  Y +K++ +VGEER K II NSL++V+ GSNDI N
Sbjct: 145 YLFIYKPLLFLKGGIALSQQLKLFEEYVEKMKKMVGEERTKLIIKNSLFMVICGSNDITN 204

Query: 195 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 254
           T++     Q  + + S+T  MA +A +F ++L+  GARRI  F  PP+GCVPSQRTLAGG
Sbjct: 205 TYFGLPSVQQQYDVASFTTLMADNARSFAQKLHEYGARRIQVFGAPPVGCVPSQRTLAGG 264

Query: 255 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
             R CV  +N+A KL+N KL + L  L   L D  ++YVDIY+ LLD+IL+  +YG
Sbjct: 265 PTRNCVVRFNDATKLYNVKLAANLGSLSRTLGDKTIIYVDIYDSLLDIILDPRQYG 320

[Close]

BLAST of Carg09388 vs. ExPASy TrEMBL
Match: A0A6J1HEF7 (GDSL esterase/lipase EXL3-like OS=Cucurbita moschata OX=3662 GN=LOC111462738 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.7e-163
Identity = 291/296 (98.31%), Postives = 293/296 (98.99%), Query Frame = 0


Query: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60
           MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC
Sbjct: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60

Query: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120
           NYPPYGRDFP+GRPTGRFSNGRVPSDLV DALGIKELLPPYADPNLQPEDLITGVNFASG
Sbjct: 61  NYPPYGRDFPSGRPTGRFSNGRVPSDLVVDALGIKELLPPYADPNLQPEDLITGVNFASG 120

Query: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180
           GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN
Sbjct: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180

Query: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240
           TFYLARIRQLHFTI SYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG
Sbjct: 181 TFYLARIRQLHFTISSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240

Query: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSR+VYVDIYNPLLDVILNY KYG
Sbjct: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRLVYVDIYNPLLDVILNYAKYG 296

[Close]

BLAST of Carg09388 vs. ExPASy TrEMBL
Match: A0A6J1KBZ3 (GDSL esterase/lipase EXL3-like OS=Cucurbita maxima OX=3661 GN=LOC111492487 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.1e-159
Identity = 286/296 (96.62%), Postives = 289/296 (97.64%), Query Frame = 0


Query: 1   MPPPSLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKC 60
           MPPPSLN MFFLSLLLLLHLRP IAAITIP GYSVPAVFVFGDSIVDTGNNNNVITQAKC
Sbjct: 42  MPPPSLNLMFFLSLLLLLHLRPTIAAITIPLGYSVPAVFVFGDSIVDTGNNNNVITQAKC 101

Query: 61  NYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASG 120
           NYPPYGRDFP G PTGRFSNGRVPSDLVADALGIK+LLPPYADPNLQPEDLITGVNFASG
Sbjct: 102 NYPPYGRDFPGGSPTGRFSNGRVPSDLVADALGIKKLLPPYADPNLQPEDLITGVNFASG 161

Query: 121 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIAN 180
           GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEG+VGEERAKFIIVNSLYLVVAGSNDIAN
Sbjct: 162 GAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGLVGEERAKFIIVNSLYLVVAGSNDIAN 221

Query: 181 TFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 240
           TFYLARIRQLHFTI SYTDFMA+SASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG
Sbjct: 222 TFYLARIRQLHFTIDSYTDFMAQSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGG 281

Query: 241 IQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           IQR CVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG
Sbjct: 282 IQRECVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 337

[Close]

BLAST of Carg09388 vs. ExPASy TrEMBL
Match: A0A6J1CSZ1 (GDSL esterase/lipase EXL3-like OS=Momordica charantia OX=3673 GN=LOC111013939 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.5e-132
Identity = 240/292 (82.19%), Postives = 258/292 (88.36%), Query Frame = 0


Query: 5   SLNFMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPP 64
           SL F  FL L LLLHL  A AAI IPP YSVPAVFVFGDSIVDTGNNNN+ITQAKCNYPP
Sbjct: 20  SLRFKLFLCLQLLLHLPAANAAIKIPPSYSVPAVFVFGDSIVDTGNNNNLITQAKCNYPP 79

Query: 65  YGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGF 124
           YG+DFP G+ TGRFSNGRVPSDLVAD LGIKELLPPYA+ NLQPEDLITGV FASGGAGF
Sbjct: 80  YGKDFPGGQATGRFSNGRVPSDLVADVLGIKELLPPYANSNLQPEDLITGVCFASGGAGF 139

Query: 125 DPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYL 184
           DPLTSQTAPAISL DQLA FR Y KKL+G+VGEERA FII NS YLVVAGSNDIANT++L
Sbjct: 140 DPLTSQTAPAISLADQLAMFREYSKKLQGLVGEERANFIIANSFYLVVAGSNDIANTYFL 199

Query: 185 ARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRA 244
           +RIRQ H+ I SYTDFMA+SAS+FVK+LYAAGARRIG F  P LGCVPSQRTLAGGI+RA
Sbjct: 200 SRIRQFHYNINSYTDFMAQSASSFVKDLYAAGARRIGLFGVPSLGCVPSQRTLAGGIERA 259

Query: 245 CVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           CV+EYN+AA+LFNGKLHSTLD LQTILP SRVVYVDIYNPLLD+I N  KYG
Sbjct: 260 CVSEYNSAAELFNGKLHSTLDQLQTILPQSRVVYVDIYNPLLDIIQNPAKYG 311

[Close]

BLAST of Carg09388 vs. ExPASy TrEMBL
Match: A0A0A0LN84 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G346080 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.5e-130
Identity = 239/300 (79.67%), Postives = 263/300 (87.67%), Query Frame = 0


Query: 2   PPPS----LNFMFFLSLLLLLHLRPAIAAI-TIPPGYSVPAVFVFGDSIVDTGNNNNVIT 61
           PPPS    L  +F   LL+ L L+P  AA   IPPGYSVPAVF+FGDSIVDTGNNNN+IT
Sbjct: 11  PPPSSLKVLKLIFQCLLLIPLLLQPLRAATDIIPPGYSVPAVFIFGDSIVDTGNNNNLIT 70

Query: 62  QAKCNYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVN 121
           QAKCNYPPYGRDFP+GRPTGRFSNGRVPSDLV D LGIK LLPPYADPNLQ EDL+TGVN
Sbjct: 71  QAKCNYPPYGRDFPDGRPTGRFSNGRVPSDLVVDVLGIKPLLPPYADPNLQLEDLLTGVN 130

Query: 122 FASGGAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSN 181
           FASGGAGFDPLTS+TAPAISL+ QLA FR Y+KK+EG+VGEE+AKFII NSL+LVVAGSN
Sbjct: 131 FASGGAGFDPLTSKTAPAISLDAQLAMFREYRKKIEGLVGEEKAKFIIDNSLFLVVAGSN 190

Query: 182 DIANTFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRT 241
           DI NTFYLAR RQ  + I +YTDFM + ASA+VK+LYAAGARRIGFF+TPPLGC+PSQRT
Sbjct: 191 DIGNTFYLARFRQGQYNIDTYTDFMIQHASAYVKDLYAAGARRIGFFATPPLGCLPSQRT 250

Query: 242 LAGGIQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           LAGGI+R CVNEYNNAAKLFNGKL +TL +LQTILPDSRVVYVDIYNPLLDVI NY KYG
Sbjct: 251 LAGGIERGCVNEYNNAAKLFNGKLQTTLGYLQTILPDSRVVYVDIYNPLLDVIQNYAKYG 310

[Close]

BLAST of Carg09388 vs. ExPASy TrEMBL
Match: A0A5A7VB65 (GDSL esterase/lipase EXL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001600 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 6.8e-128
Identity = 240/303 (79.21%), Postives = 261/303 (86.14%), Query Frame = 0


Query: 2   PPPS----LNFMFFLSLLL-LLHLRPAIAA---ITIPPGYSVPAVFVFGDSIVDTGNNNN 61
           PPPS    L    F  LLL LL  RP  AA   I IPPGYSVPAVFVFGDSIVDTGNNNN
Sbjct: 10  PPPSSSSVLKLKVFQCLLLPLLLQRPVRAATKNIIIPPGYSVPAVFVFGDSIVDTGNNNN 69

Query: 62  VITQAKCNYPPYGRDFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLIT 121
           +ITQAKCNYPPYGRDF +GRPTGRFSNGRVPSDLV D LGIK LLPPYADPNLQ EDL+T
Sbjct: 70  LITQAKCNYPPYGRDFADGRPTGRFSNGRVPSDLVVDVLGIKPLLPPYADPNLQLEDLLT 129

Query: 122 GVNFASGGAGFDPLTSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVA 181
           GVNFASGGAGFDPLTS+ APAISL+ QLA FR Y+KK+EG+VGEE+AKFII NSL+LVVA
Sbjct: 130 GVNFASGGAGFDPLTSKLAPAISLDAQLAMFREYRKKIEGLVGEEKAKFIIDNSLFLVVA 189

Query: 182 GSNDIANTFYLARIRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPS 241
           GSNDI NTFYLAR RQ  + I +YTDFM + ASA+VK+LYAAGARRIGFF+TPPLGC+PS
Sbjct: 190 GSNDIGNTFYLARFRQGQYNIDTYTDFMNQHASAYVKDLYAAGARRIGFFATPPLGCLPS 249

Query: 242 QRTLAGGIQRACVNEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYV 297
           QRTLAGGIQRACVNEYN+AAKLFNGKL +TL +LQ+ LPDSRVVYVDIYNPLLDVI NY 
Sbjct: 250 QRTLAGGIQRACVNEYNDAAKLFNGKLQTTLSYLQSTLPDSRVVYVDIYNPLLDVIQNYA 309

[Close]

BLAST of Carg09388 vs. TAIR 10
Match: AT1G75900.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 316.6 bits (810), Expect = 2.2e-86
Identity = 155/285 (54.39%), Postives = 202/285 (70.88%), Query Frame = 0


Query: 12  LSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGRDFPN 71
           + LL +L L   I A+ +PP   +PAV  FGDSIVDTG NNNV T  KC++ PYG +F +
Sbjct: 18  ICLLSVLFLTETITAVKLPPKLIIPAVIAFGDSIVDTGMNNNVKTVVKCDFLPYGINFQS 77

Query: 72  GRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQT 131
           G  TGRF +GRVP+DL+A+ LGIK ++P Y DPNL+ +DL+TGV+FASGG+G+DP+T + 
Sbjct: 78  GVATGRFCDGRVPADLLAEELGIKSIVPAYLDPNLKSKDLLTGVSFASGGSGYDPITPKL 137

Query: 132 APAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLARIRQLH 191
              ISLEDQL+ F  Y +K++ +VGE R  FI+ NSL+L+VAGS+DIANT+Y  R R   
Sbjct: 138 VAVISLEDQLSYFEEYIEKVKNIVGEARKDFIVANSLFLLVAGSDDIANTYYTLRARP-E 197

Query: 192 FTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVNEYNN 251
           + + SYT  M+ SAS FV +LY  G RR+  F  PP+GCVPSQRTL GGI R C + YN 
Sbjct: 198 YDVDSYTTLMSDSASEFVTKLYGYGVRRVAVFGAPPIGCVPSQRTLGGGILRDCADNYNE 257

Query: 252 AAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           AAKLFN KL   LD L+  LP  + +Y++IY+PL D+I N   YG
Sbjct: 258 AAKLFNSKLSPKLDSLRKTLPGIKPIYINIYDPLFDIIQNPANYG 301

[Close]

BLAST of Carg09388 vs. TAIR 10
Match: AT1G75880.2 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.4e-85
Identity = 158/289 (54.67%), Postives = 205/289 (70.93%), Query Frame = 0


Query: 8   FMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGR 67
           F     L+LL       A + IP   +VPAV VFGDSIVD GNN+++IT+A+C+Y PYG 
Sbjct: 23  FWCIFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMITEARCDYAPYGI 82

Query: 68  DFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPL 127
           DF  G  TGRFSNG+VP D+VA+ LGIK  +P Y +PNL+PE+L+TGV FASGGAG+ PL
Sbjct: 83  DFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGGAGYVPL 142

Query: 128 TSQTAPAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLARI 187
           T++ A  I L  QL  F  Y +KL+ +VGE+R KFII NSL++V+ GSNDIAN F+    
Sbjct: 143 TTKIAGGIPLPQQLIYFEEYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIANDFFTLPP 202

Query: 188 RQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVN 247
            +LH+T+ S+T  MA +A +F + LY  GARRI  F  PP+GCVPSQRT+AGG  R CV 
Sbjct: 203 VRLHYTVASFTALMADNARSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGGPTRDCVA 262

Query: 248 EYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
            +N+AAKLFN KL + +D L   L D  ++Y+DIY+PLLD+ILN  +YG
Sbjct: 263 RFNDAAKLFNTKLSANIDVLSRTLQDPTIIYIDIYSPLLDLILNPHQYG 311

[Close]

BLAST of Carg09388 vs. TAIR 10
Match: AT1G75880.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 308.5 bits (789), Expect = 5.9e-84
Identity = 158/290 (54.48%), Postives = 205/290 (70.69%), Query Frame = 0


Query: 8   FMFFLSLLLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGR 67
           F     L+LL       A + IP   +VPAV VFGDSIVD GNN+++IT+A+C+Y PYG 
Sbjct: 23  FWCIFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMITEARCDYAPYGI 82

Query: 68  DFPNGRPTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPL 127
           DF  G  TGRFSNG+VP D+VA+ LGIK  +P Y +PNL+PE+L+TGV FASGGAG+ PL
Sbjct: 83  DFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGGAGYVPL 142

Query: 128 TSQTA-PAISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLAR 187
           T++ A   I L  QL  F  Y +KL+ +VGE+R KFII NSL++V+ GSNDIAN F+   
Sbjct: 143 TTKIAVGGIPLPQQLIYFEEYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIANDFFTLP 202

Query: 188 IRQLHFTIGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACV 247
             +LH+T+ S+T  MA +A +F + LY  GARRI  F  PP+GCVPSQRT+AGG  R CV
Sbjct: 203 PVRLHYTVASFTALMADNARSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGGPTRDCV 262

Query: 248 NEYNNAAKLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
             +N+AAKLFN KL + +D L   L D  ++Y+DIY+PLLD+ILN  +YG
Sbjct: 263 ARFNDAAKLFNTKLSANIDVLSRTLQDPTIIYIDIYSPLLDLILNPHQYG 312

[Close]

BLAST of Carg09388 vs. TAIR 10
Match: AT5G42170.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 301.6 bits (771), Expect = 7.2e-82
Identity = 147/270 (54.44%), Postives = 192/270 (71.11%), Query Frame = 0


Query: 27  ITIPPGYSVPAVFVFGDSIVDTGNNNNVITQAKCNYPPYGRDFPNGRPTGRFSNGRVPSD 86
           I +PP  ++P +  FGDSIVD+GNNN++ T  KCN+PPYG+DFP    TGRFS+GRVPSD
Sbjct: 40  IKLPPNVTIPGIITFGDSIVDSGNNNHLRTALKCNFPPYGKDFPGKIATGRFSDGRVPSD 99

Query: 87  LVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQTAPAISLEDQLANFRA 146
           +VA+ LGI E +P Y +P L+ EDL+ GVNFASGG+G+DPLT++    +SL DQL NF+ 
Sbjct: 100 IVAERLGIAETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLVKVVSLSDQLKNFQE 159

Query: 147 YKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLARIRQLHFTIGSYTDFMARSAS 206
           YK KL+ +VGEE+A F++ NSLYLVVA SNDIA+T+     R + +   SY D++A SAS
Sbjct: 160 YKNKLKVIVGEEKANFLVKNSLYLVVASSNDIAHTY---TARSIKYNKTSYADYLADSAS 219

Query: 207 AFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVNEYNNAAKLFNGKLHSTLDH 266
            FV  LY  GARRIG FS  P+GCVP+ RTL G ++R C  + N  A+ FN K+  TL+ 
Sbjct: 220 KFVSALYGLGARRIGVFSAVPVGCVPAARTLRGKLKRRCSEKLNEVARNFNAKISPTLEA 279

Query: 267 LQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           L   LPDSRVV +D+ + L D+I N   YG
Sbjct: 280 LGKELPDSRVVLIDVCDTLNDMIENPKNYG 306

[Close]

BLAST of Carg09388 vs. TAIR 10
Match: AT1G75890.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 9.8e-79
Identity = 146/283 (51.59%), Postives = 194/283 (68.55%), Query Frame = 0


Query: 15  LLLLHLRPAIAAITIPPGYSVPAVFVFGDSIVDTGNNNNVITQ-AKCNYPPYGRDFPNGR 74
           L+LL      A +  PP  + PA+ VFGDSIVD GNN++++T  A+CNYPPYG DF  G 
Sbjct: 25  LVLLCKTSTNALVKQPPNETTPAIIVFGDSIVDAGNNDDIMTTLARCNYPPYGIDFDGGI 84

Query: 75  PTGRFSNGRVPSDLVADALGIKELLPPYADPNLQPEDLITGVNFASGGAGFDPLTSQTAP 134
           PTGRF NG+V +D +A   GIK  +P Y +PNL+PEDL+TGV FASGGAG+ P T+Q + 
Sbjct: 85  PTGRFCNGKVATDFIAGKFGIKPSIPAYRNPNLKPEDLLTGVTFASGGAGYVPFTTQLSG 144

Query: 135 AISLEDQLANFRAYKKKLEGVVGEERAKFIIVNSLYLVVAGSNDIANTFYLARIRQLHFT 194
            I+L  QL  F  Y +K++ +VGEER K II NSL++V+ GSNDI NT++     Q  + 
Sbjct: 145 GIALSQQLKLFEEYVEKMKKMVGEERTKLIIKNSLFMVICGSNDITNTYFGLPSVQQQYD 204

Query: 195 IGSYTDFMARSASAFVKELYAAGARRIGFFSTPPLGCVPSQRTLAGGIQRACVNEYNNAA 254
           + S+T  MA +A +F ++L+  GARRI  F  PP+GCVPSQRTLAGG  R CV  +N+A 
Sbjct: 205 VASFTTLMADNARSFAQKLHEYGARRIQVFGAPPVGCVPSQRTLAGGPTRNCVVRFNDAT 264

Query: 255 KLFNGKLHSTLDHLQTILPDSRVVYVDIYNPLLDVILNYVKYG 297
           KL+N KL + L  L   L D  ++YVDIY+ LLD+IL+  +YG
Sbjct: 265 KLYNVKLAANLGSLSRTLGDKTIIYVDIYDSLLDIILDPRQYG 307

The following BLAST results are available for this feature:

BLAST of Carg09388 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029454.1	6.4e-173	100.00	GDSL esterase/lipase EXL3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6598518.1	3.8e-165	99.66	GDSL esterase/lipase EXL3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022962225.1	3.5e-163	98.31	GDSL esterase/lipase EXL3-like [Cucurbita moschata]	[more]
XP_023545211.1	1.5e-161	97.64	GDSL esterase/lipase EXL3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997604.1	2.4e-159	96.62	GDSL esterase/lipase EXL3-like [Cucurbita maxima]	[more]

BLAST of Carg09388 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94CH6	3.1e-85	54.39	GDSL esterase/lipase EXL3 OS=Arabidopsis thaliana OX=3702 GN=EXL3 PE=2 SV=1	[more]
Q94CH8	8.3e-83	54.48	GDSL esterase/lipase EXL1 OS=Arabidopsis thaliana OX=3702 GN=EXL1 PE=2 SV=1	[more]
Q9FHW9	1.0e-80	54.44	GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana OX=3702 GN=At5g42170/At5g...	[more]
Q9LH73	3.7e-75	51.33	GDSL esterase/lipase At3g14820 OS=Arabidopsis thaliana OX=3702 GN=At3g14820 PE=3...	[more]
Q94CH7	6.4e-75	49.32	GDSL esterase/lipase EXL2 OS=Arabidopsis thaliana OX=3702 GN=EXL2 PE=2 SV=1	[more]

BLAST of Carg09388 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HEF7	1.7e-163	98.31	GDSL esterase/lipase EXL3-like OS=Cucurbita moschata OX=3662 GN=LOC111462738 PE=...	[more]
A0A6J1KBZ3	1.1e-159	96.62	GDSL esterase/lipase EXL3-like OS=Cucurbita maxima OX=3661 GN=LOC111492487 PE=3 ...	[more]
A0A6J1CSZ1	4.5e-132	82.19	GDSL esterase/lipase EXL3-like OS=Momordica charantia OX=3673 GN=LOC111013939 PE...	[more]
A0A0A0LN84	2.5e-130	79.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G346080 PE=3 SV=1	[more]
A0A5A7VB65	6.8e-128	79.21	GDSL esterase/lipase EXL3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]

BLAST of Carg09388 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75900.1	2.2e-86	54.39	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G75880.2	2.4e-85	54.67	SGNH hydrolase-type esterase superfamily protein	[more]
AT1G75880.1	5.9e-84	54.48	SGNH hydrolase-type esterase superfamily protein	[more]
AT5G42170.1	7.2e-82	54.44	SGNH hydrolase-type esterase superfamily protein	[more]
AT1G75890.2	9.8e-79	51.59	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 38..286 e-value: 1.8E-28 score: 100.5
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 32..305 e-value: 6.5E-59 score: 201.9
None	No IPR available	PANTHER	PTHR45642:SF95	GDSL-LIKE LIPASE/ACYLHYDROLASE	coord: 24..298
None	No IPR available	PANTHER	PTHR45642	GDSL ESTERASE/LIPASE EXL3	coord: 24..298
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 37..254
IPR008265	Lipase, GDSL, active site	PROSITE	PS01098	LIPASE_GDSL_SER	coord: 38..49
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 36..303 e-value: 1.15425E-123 score: 354.614

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09388-RA	Carg09388-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016298	lipase activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds