Carg09360 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09360
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionU-box domain-containing protein 13-like
LocationCarg_Chr05: 1662439 .. 1665895 (-)
RNA-Seq ExpressionCarg09360
SyntenyCarg09360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTGAAACTGAAAACCACTGTTTTGGATTATTCCGCCACCGCCACCGCCACCGTTTTCGGGTTCTTCTTATGTTTTTTTGCTTTGCAATGTAGTGCGCTGTAAGAATTGGTTATAGGGTAATGGCCAAGTGTCAGTCCAACAGCCTTGGATCATTGGTAATCGATGGTATCGCCGGCGCCAATGCAGGTACTCGACACTTCCGGTTTTGTACTGCGTTTTCTGGAGCTGCATTCCGTCGGAGAATCTATGACGCTGTTAGTTGCGGTGGGAGTTCTCGGTATCGCCATCGGTACAACGATGAGATGATGGATGGTGAGCAGAGCACGGGGAACAGATCGGCTTCGGAGACTAGGGATTTGAAGGAGGCGGAGCAGACGAGGAATCCGAAACTGGCTAATGGAAGATCCGAGAAGCTTGTGGATCTTTTGAATTTGGCGGAGTCGATGGAGGCGGAGAATGAGGAGGAGACTAGAAGGAAGGAGGAGGAGTTGGAGGAGTTGAAACGCACGGTGGAGGATTTGCAGGCGGAGGATTTGGTGAGGCGAAAGTCGGCGGCTAGCAAAGTGAGGCTTATGGCGAAGGAGGATTTGGTAATACGAGGGACGCTTGCTCTTCTTGGAGCCATTCCTCCTCTCGTTGCGATGGTTGATTTGGAAGATGAACAATCTCAGATCGCTGCGCTTTACGCACTGCTAAATCTTGGAATTGGCAACAATGCGTAAGATTCTTTAGGATTCCATTTCTTCACTCTTCATACTTGCCAGTCGATTTTCATTTTCTCTGTTCTCAGTTTCTAACTCTCCTTGAAAATTCCCCTCGATTTCAACCTATTGCATATTGTTTCCTGCATGCATGTTTTAGTTGATTTACTCGATCCATATATTTCTTAAATTCCCCCACATTTTCCATTACCATCTCTGCTTTTCTCCTATTTCTGTCACTGTCTTTTCTGAACTATGATTTCTTGAGGGGACTTCTTCCGCTTTTCGCTTCCGTTCAAAATGGCAAGTCAGAGTAGCAGTCTCTTTGATGATTGATCCAACTCCTTTCTTCTGGTAAAAACCAAAACAGAGACCATGTACTGCGCTGCATATTTCGAGAAATAGCTTCGCGTCTCCCTGCTGCTGCTTTTCATGTACCCTTCTGGGGAAAAAGATAATAAAAACCTAGCAATTTCCTTATTTCTTTCGTCTTCCAGTTAGAAATTTCAATGTGTTAAACCTCAATATTTGTATCGAGTAAGTTCTTAGTACTCCATTGACGTTGTTTTGTCCTAATCATTGCAGGAACAAGGCAGCAATTGTGAAAGTGGGGGTCATCCACAAAATGTTAAAGCTCATTAAATCAGAGAGTGCATCAAACTCATTGGTTACGGAGGCAATAATTGCAAACTTTCTCGGCTTGAGCGCTCTGGACTCGAACAAGCCCGTAATTGGATCGTCCGGTGCGATTCCCTTCTTGGTCAAATCCCTACAAAACACCGACCATAAAATTAGCAATCAGGCTCGGCAGGATGCTCTAAGGGCGCTCTTCAATCTCTCAATCGCCGCATCAAATATCTCAATAATATTAGAAACGGATTTCATCCCATTTCTTCTTAACATGTTAGGAGACATGGAAGTGAGTGAAAGGATCCTTTCAATTTTAAGCAACGTGGTATCAACCCGAGAGGGTAGACGAGCAATAAGCGTCGTTCCAGACGCGTTCCCAATATTAGTTGATGTCTTAAACTGGACAGATTCACCTGGGTGTCAAGAAAAGGCGTCCTACGTTTTAATGGTGATGGCACACAAGCTATATGGTGAAAGACAGACAATGGTAGAAGCAGGATTAGTATCTGCTTCTCTTGAATTAACGCTCATGGGTAGTGCATTGGCTCAGAAAAGAGCATCCAGGATTTTGGAGTGCTTGAGATATGATAAAGGGAAGCAAGTTTCTGAAAGTTTTGGTGGGAATCTGGGTGTTGCACCAGTGTCTGCTCCCATTATTGGGACTTCATCTTCTTCAAATTGCAATTCAGATTCTAAAAGGTACCCAGAAGAATCTGAAGAGGCCATGAGCGTGGAGAAAAAGGCGGTGAAGCAACTAGTCCAACAGAGTCTACAGTACAACATGAGGAAAATTGTGAAGAGGGCCAATCTGCCTCAAGATTTTGCTCCTTCAGAGCATTTTAAGTCGCTTACTGCGAGTTCAACTTCGAAAAGCCTACCATTTTAGCATAGTAGACTACCATGTTAGATCTGTACCTAGTGTCATCATCAGGAAGAAGTTAGCCTCCTCTTTTAAGTTATTAAATAGCAGTCAGTTTCGTTAGAGAAGTGGTTTAGCCTTTTAGTTCTTTGGTTAGTGTAAAAAATAAATAAAGGACCTACCTCTGTTTCCAAATTCAATCCTCAGACAACAACCAGATCCAGAACCAAATACCTCGATAGCTTCCTCGGCCATGGAATTGTCTGAAAATTGAAAATTCCAGTGGCTGTTTTCACTGAGCATAACAAAGCTTGGGGCACTGCTCAGTGGGCCATGTTCAACTCACCATCACAATCCACCCTACCTCGGTGGCCCCTTTAAAATGTAATGTAATGTAATGCATATCTCATATTTTCCCTTTGCTGGCCAGGCCTCTTGAAACGAGTGTCGGTCGCTTTCACGTGCTTTTTTACACTCAACAAGTTGGGTTGTGAGATTCAAATCTCGAGCATGTCTTTACCAGTTGAACTACGTTCAAATTAACATATTTTCTCATCCATTTTTTAACCATGCACAGATCTGGAGCAGGGAACTTGCTGAGCTGTTGTTGGCAGAGGCAAAATAATGTGGAAGGGATGTCTTGAGCACGAGGTATTGTTTTGTAAATATAGAATTGAGTGAACTCATTATTCTATTAGTGCTGCAGTTCTGTCTTATTCTTTACTTTTGTGATGGCCCCATATTGTATATTTTTCTCCTGTTGGCCTGTAGCAACTTTAGATCATATTTTTCTCTACTGTGAACAGTTTGTGAACCCTTGACCTGTAATTCCATGTGGTTTGACAAATTCATCTCACAGCTGTGATATGAAATGGTTAGCTATTTTCTATGTTCAAATTCTGCTGGGTTGATCTGCTAGTGGGGTCTCCTTTTATTAATGTCATTGAAGAATTCCAATCCCATCGTACATCACAAGATTAGCTGTGTAACGATAACAAAATCTATGTGAGACAAAAGGACTTTTCTCACAAGTGGAAGTAAAGATTAATAATTTGAACTTTTGAATCGAAACCTCTAGTGGATGGACTTATTATATGATTCCAAAAGGAAATCATACAAACTAAATGTGCTAGAGCTAGGCAACTAAACCCGTAGAACGAGTTCTTGAGACAAATCTCTGCTTATCATTTTTCTTCACTAGAGACGACTCTGATCCAAAAGAAACATGTGGTTTCTAAACCCATTGAGATGACCCAAGAATTTCA

mRNA sequence

TTCTGAAACTGAAAACCACTGTTTTGGATTATTCCGCCACCGCCACCGCCACCGTTTTCGGGTTCTTCTTATGTTTTTTTGCTTTGCAATGTAGTGCGCTGTAAGAATTGGTTATAGGGTAATGGCCAAGTGTCAGTCCAACAGCCTTGGATCATTGGTAATCGATGGTATCGCCGGCGCCAATGCAGGTACTCGACACTTCCGGTTTTGTACTGCGTTTTCTGGAGCTGCATTCCGTCGGAGAATCTATGACGCTGTTAGTTGCGGTGGGAGTTCTCGGTATCGCCATCGGTACAACGATGAGATGATGGATGGTGAGCAGAGCACGGGGAACAGATCGGCTTCGGAGACTAGGGATTTGAAGGAGGCGGAGCAGACGAGGAATCCGAAACTGGCTAATGGAAGATCCGAGAAGCTTGTGGATCTTTTGAATTTGGCGGAGTCGATGGAGGCGGAGAATGAGGAGGAGACTAGAAGGAAGGAGGAGGAGTTGGAGGAGTTGAAACGCACGGTGGAGGATTTGCAGGCGGAGGATTTGGTGAGGCGAAAGTCGGCGGCTAGCAAAGTGAGGCTTATGGCGAAGGAGGATTTGGTAATACGAGGGACGCTTGCTCTTCTTGGAGCCATTCCTCCTCTCGTTGCGATGGTTGATTTGGAAGATGAACAATCTCAGATCGCTGCGCTTTACGCACTGCTAAATCTTGGAATTGGCAACAATGCGAACAAGGCAGCAATTGTGAAAGTGGGGGTCATCCACAAAATGTTAAAGCTCATTAAATCAGAGAGTGCATCAAACTCATTGGTTACGGAGGCAATAATTGCAAACTTTCTCGGCTTGAGCGCTCTGGACTCGAACAAGCCCGTAATTGGATCGTCCGGTGCGATTCCCTTCTTGGTCAAATCCCTACAAAACACCGACCATAAAATTAGCAATCAGGCTCGGCAGGATGCTCTAAGGGCGCTCTTCAATCTCTCAATCGCCGCATCAAATATCTCAATAATATTAGAAACGGATTTCATCCCATTTCTTCTTAACATGTTAGGAGACATGGAAGTGAGTGAAAGGATCCTTTCAATTTTAAGCAACGTGGTATCAACCCGAGAGGGTAGACGAGCAATAAGCGTCGTTCCAGACGCGTTCCCAATATTAGTTGATGTCTTAAACTGGACAGATTCACCTGGGTGTCAAGAAAAGGCGTCCTACGTTTTAATGGTGATGGCACACAAGCTATATGGTGAAAGACAGACAATGGTAGAAGCAGGATTAGTATCTGCTTCTCTTGAATTAACGCTCATGGGTAGTGCATTGGCTCAGAAAAGAGCATCCAGGATTTTGGAGTGCTTGAGATATGATAAAGGGAAGCAAGTTTCTGAAAGTTTTGGTGGGAATCTGGGTGTTGCACCAGTGTCTGCTCCCATTATTGGGACTTCATCTTCTTCAAATTGCAATTCAGATTCTAAAAGGTACCCAGAAGAATCTGAAGAGGCCATGAGCGTGGAGAAAAAGGCGGTGAAGCAACTAGTCCAACAGAGTCTACAGTACAACATGAGGAAAATTGTGAAGAGGGCCAATCTGCCTCAAGATTTTGCTCCTTCAGAGCATTTTAAGTCGCTTACTGCGAGTTCAACTTCGAAAAGCCTACCATTTTAGCATAGTAGACTACCATGTTAGATCTGTACCTAGTGTCATCATCAGGAAGAAGTTAGCCTCCTCTTTTAAGTTATTAAATAGCAGTCAGTTTCGTTAGAGAAGTGGTTTAGCCTTTTAGTTCTTTGGTTAGTGTAAAAAATAAATAAAGGACCTACCTCTGTTTCCAAATTCAATCCTCAGACAACAACCAGATCCAGAACCAAATACCTCGATAGCTTCCTCGGCCATGGAATTGTCTGAAAATTGAAAATTCCAGTGGCTGTTTTCACTGAGCATAACAAAGCTTGGGGCACTGCTCAGTGGGCCATGTTCAACTCACCATCACAATCCACCCTACCTCGGTGGCCCCTTTAAAATATCTGGAGCAGGGAACTTGCTGAGCTGTTGTTGGCAGAGGCAAAATAATGTGGAAGGGATGTCTTGAGCACGAGGTATTGTTTTGTAAATATAGAATTGAGTGAACTCATTATTCTATTAGTGCTGCAGTTCTGTCTTATTCTTTACTTTTGTGATGGCCCCATATTGTATATTTTTCTCCTGTTGGCCTGTAGCAACTTTAGATCATATTTTTCTCTACTGTGAACAGTTTGTGAACCCTTGACCTGTAATTCCATGTGGTTTGACAAATTCATCTCACAGCTGTGATATGAAATGGTTAGCTATTTTCTATGTTCAAATTCTGCTGGGTTGATCTGCTAGTGGGGTCTCCTTTTATTAATGTCATTGAAGAATTCCAATCCCATCGTACATCACAAGATTAGCTGTGTAACGATAACAAAATCTATGTGAGACAAAAGGACTTTTCTCACAAGTGGAAGTAAAGATTAATAATTTGAACTTTTGAATCGAAACCTCTAGTGGATGGACTTATTATATGATTCCAAAAGGAAATCATACAAACTAAATGTGCTAGAGCTAGGCAACTAAACCCGTAGAACGAGTTCTTGAGACAAATCTCTGCTTATCATTTTTCTTCACTAGAGACGACTCTGATCCAAAAGAAACATGTGGTTTCTAAACCCATTGAGATGACCCAAGAATTTCA

Coding sequence (CDS)

ATGGCCAAGTGTCAGTCCAACAGCCTTGGATCATTGGTAATCGATGGTATCGCCGGCGCCAATGCAGGTACTCGACACTTCCGGTTTTGTACTGCGTTTTCTGGAGCTGCATTCCGTCGGAGAATCTATGACGCTGTTAGTTGCGGTGGGAGTTCTCGGTATCGCCATCGGTACAACGATGAGATGATGGATGGTGAGCAGAGCACGGGGAACAGATCGGCTTCGGAGACTAGGGATTTGAAGGAGGCGGAGCAGACGAGGAATCCGAAACTGGCTAATGGAAGATCCGAGAAGCTTGTGGATCTTTTGAATTTGGCGGAGTCGATGGAGGCGGAGAATGAGGAGGAGACTAGAAGGAAGGAGGAGGAGTTGGAGGAGTTGAAACGCACGGTGGAGGATTTGCAGGCGGAGGATTTGGTGAGGCGAAAGTCGGCGGCTAGCAAAGTGAGGCTTATGGCGAAGGAGGATTTGGTAATACGAGGGACGCTTGCTCTTCTTGGAGCCATTCCTCCTCTCGTTGCGATGGTTGATTTGGAAGATGAACAATCTCAGATCGCTGCGCTTTACGCACTGCTAAATCTTGGAATTGGCAACAATGCGAACAAGGCAGCAATTGTGAAAGTGGGGGTCATCCACAAAATGTTAAAGCTCATTAAATCAGAGAGTGCATCAAACTCATTGGTTACGGAGGCAATAATTGCAAACTTTCTCGGCTTGAGCGCTCTGGACTCGAACAAGCCCGTAATTGGATCGTCCGGTGCGATTCCCTTCTTGGTCAAATCCCTACAAAACACCGACCATAAAATTAGCAATCAGGCTCGGCAGGATGCTCTAAGGGCGCTCTTCAATCTCTCAATCGCCGCATCAAATATCTCAATAATATTAGAAACGGATTTCATCCCATTTCTTCTTAACATGTTAGGAGACATGGAAGTGAGTGAAAGGATCCTTTCAATTTTAAGCAACGTGGTATCAACCCGAGAGGGTAGACGAGCAATAAGCGTCGTTCCAGACGCGTTCCCAATATTAGTTGATGTCTTAAACTGGACAGATTCACCTGGGTGTCAAGAAAAGGCGTCCTACGTTTTAATGGTGATGGCACACAAGCTATATGGTGAAAGACAGACAATGGTAGAAGCAGGATTAGTATCTGCTTCTCTTGAATTAACGCTCATGGGTAGTGCATTGGCTCAGAAAAGAGCATCCAGGATTTTGGAGTGCTTGAGATATGATAAAGGGAAGCAAGTTTCTGAAAGTTTTGGTGGGAATCTGGGTGTTGCACCAGTGTCTGCTCCCATTATTGGGACTTCATCTTCTTCAAATTGCAATTCAGATTCTAAAAGGTACCCAGAAGAATCTGAAGAGGCCATGAGCGTGGAGAAAAAGGCGGTGAAGCAACTAGTCCAACAGAGTCTACAGTACAACATGAGGAAAATTGTGAAGAGGGCCAATCTGCCTCAAGATTTTGCTCCTTCAGAGCATTTTAAGTCGCTTACTGCGAGTTCAACTTCGAAAAGCCTACCATTTTAG

Protein sequence

MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYNDEMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRKEEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLEDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFIPFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESFGGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIVKRANLPQDFAPSEHFKSLTASSTSKSLPF
Homology
BLAST of Carg09360 vs. NCBI nr
Match: KAG7029482.1 (U-box domain-containing protein 13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 936.8 bits (2420), Expect = 7.9e-269
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND
Sbjct: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK
Sbjct: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED
Sbjct: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS
Sbjct: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI
Sbjct: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           KRANLPQDFAPSEHFKSLTASSTSKSLPF
Sbjct: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 509

BLAST of Carg09360 vs. NCBI nr
Match: XP_022962384.1 (U-box domain-containing protein 14-like [Cucurbita moschata])

HSP 1 Score: 919.8 bits (2376), Expect = 1.0e-263
Identity = 502/509 (98.62%), Postives = 503/509 (98.82%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRY HRYND
Sbjct: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYGHRYND 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           EMMD EQSTGNRSASETRDLKEAEQ RN KLANGRSEKLVDLLNLAESME ENEEETRRK
Sbjct: 61  EMMDDEQSTGNRSASETRDLKEAEQPRNLKLANGRSEKLVDLLNLAESMETENEEETRRK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED
Sbjct: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS
Sbjct: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI
Sbjct: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGLVSASLELTL+GSALAQKRASRIL CLRYDKGKQVSESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLLGSALAQKRASRILGCLRYDKGKQVSESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           KRANLPQDFAPSEHFKSLTASSTSKSLPF
Sbjct: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 509

BLAST of Carg09360 vs. NCBI nr
Match: XP_023545656.1 (U-box domain-containing protein 14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 918.3 bits (2372), Expect = 2.9e-263
Identity = 499/509 (98.04%), Postives = 502/509 (98.62%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKC SNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND
Sbjct: 1   MAKCHSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           EMMD EQSTGNRSASETRDLKEAEQ RNPKLANGRSEKLVDLLNLAESME ENEEETRRK
Sbjct: 61  EMMDDEQSTGNRSASETRDLKEAEQPRNPKLANGRSEKLVDLLNLAESMETENEEETRRK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EEELEELKRTVEDLQ EDLVRRK AASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED
Sbjct: 121 EEELEELKRTVEDLQTEDLVRRKLAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           EQSQIAALYALLNLGIGNNANKAAIV+VGVIHKMLKLIKSESASNSLVTEAIIANFLGLS
Sbjct: 181 EQSQIAALYALLNLGIGNNANKAAIVEVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI
Sbjct: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGL+SASLELTL+GSALAQKRASRILECLRYDKGKQVSESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLISASLELTLLGSALAQKRASRILECLRYDKGKQVSESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGNLGVA VSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNLGVAAVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           KRANLPQDFAPSEHFKSLTASSTSKSLPF
Sbjct: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 509

BLAST of Carg09360 vs. NCBI nr
Match: XP_022996942.1 (U-box domain-containing protein 13-like [Cucurbita maxima])

HSP 1 Score: 917.1 bits (2369), Expect = 6.5e-263
Identity = 498/509 (97.84%), Postives = 502/509 (98.62%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND
Sbjct: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           EMMD EQSTGNRSAS++RDLKEAEQ RNPKLANGRSEKLVDLLNLAESME ENEEETRRK
Sbjct: 61  EMMDDEQSTGNRSASDSRDLKEAEQPRNPKLANGRSEKLVDLLNLAESMETENEEETRRK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EEELEELKRTVEDLQ EDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED
Sbjct: 121 EEELEELKRTVEDLQVEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           E SQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS
Sbjct: 181 EHSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIA SNISIILETDFI
Sbjct: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAVSNISIILETDFI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGLVS+SLELTL+GSALAQKRASRILECLRYDKGKQVSESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLVSSSLELTLLGSALAQKRASRILECLRYDKGKQVSESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGNLGVA VSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNLGVAAVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           KRANLPQDFAPSEHFKSLTASSTSKSLPF
Sbjct: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 509

BLAST of Carg09360 vs. NCBI nr
Match: KAG6598549.1 (U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897.1 bits (2317), Expect = 6.9e-257
Identity = 488/495 (98.59%), Postives = 489/495 (98.79%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRY HRYND
Sbjct: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYGHRYND 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           EMMD EQSTGNRSASETRDLKEAEQ RN KLANGRSEKLVDLLNLAESME ENEEETRRK
Sbjct: 61  EMMDDEQSTGNRSASETRDLKEAEQPRNLKLANGRSEKLVDLLNLAESMETENEEETRRK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED
Sbjct: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS
Sbjct: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI
Sbjct: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPD FPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDTFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGLVSASLELTL+GSALAQKRASRILECLRYDKGKQVSESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLLGSALAQKRASRILECLRYDKGKQVSESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHF 496
           KRANLPQDFAPSEHF
Sbjct: 481 KRANLPQDFAPSEHF 495

BLAST of Carg09360 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 7.9e-22
Identity = 87/279 (31.18%), Postives = 152/279 (54.48%), Query Frame = 0

Query: 141 RRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLEDEQSQIAALYALLNLGIGNNA 200
           ++++AA ++RL+AK ++  R  +A  GAIP LV ++   D ++Q  ++ ALLNL I N  
Sbjct: 361 QQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSI-NEG 420

Query: 201 NKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLSALDSNKPVIGSSGAIPFLVK 260
           NK AIV  G I  +++++K+ S       E   A    LS +D NK  IG++GAI  L+ 
Sbjct: 421 NKGAIVDAGAITDIVEVLKNGSME---ARENAAATLFSLSVIDENKVAIGAAGAIQALIS 480

Query: 261 SLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFIPFLLNMLGDM--EVSERILS 320
            L+      + + ++DA  A+FNL I   N S  ++   +  L  +L D    + +  L+
Sbjct: 481 LLEEG----TRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALA 540

Query: 321 ILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKASYVLMVMAHKLYGERQTMV 380
           IL+ + + +EG+ AI+   ++ P+LV+++  T SP  +E A+ +L  +            
Sbjct: 541 ILAILSTNQEGKTAIAEA-ESIPVLVEIIR-TGSPRNRENAAAILWYLCIGNIERLNVAR 600

Query: 381 EAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVS 418
           E G   A  ELT  G+  A+++A+ +LE ++  +G  V+
Sbjct: 601 EVGADVALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

BLAST of Carg09360 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 100.1 bits (248), Expect = 7.4e-20
Identity = 104/345 (30.14%), Postives = 180/345 (52.17%), Query Frame = 0

Query: 73  SASETRDLKEAEQTRNPKLANGR-----SEKLVDLLNLAESMEAENEEETRRKEEELE-E 132
           SA+  RDL +       +   G+     SE+L   +  A S       ETRR   E+E +
Sbjct: 489 SAATRRDLSDFSPKFMDRRTRGQFWRRPSERLGSRIVSAPS------NETRRDLSEVETQ 548

Query: 133 LKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLEDEQSQIA 192
           +K+ VE+L++  L  ++ A +++RL+AK ++  R  +   GAI  LV ++   D  +Q  
Sbjct: 549 VKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQEN 608

Query: 193 ALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLSALDSNK 252
           A+ ALLNL I +N NK AI   G I  ++ ++++ S   S   E   A    LS ++ NK
Sbjct: 609 AVTALLNLSINDN-NKKAIADAGAIEPLIHVLENGS---SEAKENSAATLFSLSVIEENK 668

Query: 253 PVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFIPFLLNM 312
             IG SGAI  LV  L N     + + ++DA  ALFNLSI   N ++I+++  + +L+++
Sbjct: 669 IKIGQSGAIGPLVDLLGNG----TPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDL 728

Query: 313 LGDME-VSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKASYVLMV 372
           +     + ++ +++L+N+ +  EGR AI       P+LV+V+    + G +  A+ +L +
Sbjct: 729 MDPAAGMVDKAVAVLANLATIPEGRNAIG-QEGGIPLLVEVVELGSARGKENAAAALLQL 788

Query: 373 MAHKLYGERQTMV-EAGLVSASLELTLMGSALAQKRASRILECLR 410
             +   G    MV + G V   + L+  G+  A+++A  +L   R
Sbjct: 789 STNS--GRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFR 816

BLAST of Carg09360 vs. ExPASy Swiss-Prot
Match: Q9CAG5 (U-box domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=PUB7 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.3e-19
Identity = 99/358 (27.65%), Postives = 178/358 (49.72%), Query Frame = 0

Query: 63  MDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRKEE 122
           +   +ST ++S +     K       P   NG +  +V+  N  ES  +++++E      
Sbjct: 363 LSDSESTKSQSVNSIGSYKLKGVKIVPLEENGTT--VVERQNTEESFVSDDDDEEDSDIN 422

Query: 123 ELEELKRTVEDLQAEDLVRRK-SAASKVRLMAKEDLVIRGTLALLGAIPPLV----AMVD 182
            LE  +  +  L  E+ + +K     K+RL+ K+D   R  +   G +  L+    + VD
Sbjct: 423 VLERYQDLLAVLNEEEGLEKKCKVVEKIRLLLKDDEEARIFMGANGFVEALLRFLGSAVD 482

Query: 183 LEDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFL 242
             +  +Q +   AL NL + NN NK  ++  GVI  + K+I S  +  S       A +L
Sbjct: 483 DNNAAAQDSGAMALFNLAVNNNRNKELMLTSGVIRLLEKMISSAESHGSAT-----ALYL 542

Query: 243 GLSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILET 302
            LS LD  K VIGSS A+PFLV+ LQ    +I  Q + DAL AL+NLS  + NI  +L +
Sbjct: 543 NLSCLDEAKSVIGSSQAVPFLVQLLQK---EIETQCKLDALHALYNLSTYSPNIPALLSS 602

Query: 303 DFIPFLLNML---GDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPG 362
           + I  L  +L   G+    E+ L++L N+ S++EG+            L  VL+  D+  
Sbjct: 603 NIIKSLQGLLASTGENLWIEKSLAVLLNLASSQEGKDEAVSSQGMISSLATVLDMGDTTE 662

Query: 363 CQEKASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDK 413
            QE+A   L+++ +      Q +++ G++ + + +++ G+   ++++ ++L   R ++
Sbjct: 663 -QEQAVSCLLILCNGRESCIQMVLQEGVIPSLVSISVNGTPRGREKSQKLLMLFREER 709

BLAST of Carg09360 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-19
Identity = 99/330 (30.00%), Postives = 162/330 (49.09%), Query Frame = 0

Query: 81  KEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRKEEELEELKRTVEDLQAEDLV 140
           K  + +R+ K A         L++L   + + N++E R                      
Sbjct: 304 KNKQNSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQR---------------------- 363

Query: 141 RRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLEDEQSQIAALYALLNLGIGNNA 200
              +AA ++RL+AK ++  R  +A  GAIP LV ++   D ++Q  A+ ALLNL I  N 
Sbjct: 364 ---AAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVTALLNLSIHEN- 423

Query: 201 NKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLSALDSNKPVIGSSGAIPFLVK 260
           NKA+IV    I K+++++K+ S       E   A    LS +D NK  IG++GAIP L+ 
Sbjct: 424 NKASIVDSHAIPKIVEVLKTGSME---TRENAAATLFSLSVVDENKVTIGAAGAIPPLIN 483

Query: 261 SLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFIPFLLNMLGDME--VSERILS 320
            L +     S + ++DA  A+FNL I   N    ++   +  L+N L D    + +  LS
Sbjct: 484 LLCDG----SPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALS 543

Query: 321 ILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKASYVLMVMAHKLYGERQTMV 380
           +LS +    EG+  I+   +  P LV+V+  T SP  +E A+ +L ++      +     
Sbjct: 544 LLSILAGNPEGKIVIA-RSEPIPPLVEVIK-TGSPRNRENAAAILWLLCSADTEQTLAAK 598

Query: 381 EAGLVSASLELTLMGSALAQKRASRILECL 409
            AG+  A  EL+  G+  A+++AS ILE +
Sbjct: 604 AAGVEDALKELSETGTDRAKRKASSILELM 598

BLAST of Carg09360 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 1.4e-18
Identity = 85/290 (29.31%), Postives = 147/290 (50.69%), Query Frame = 0

Query: 123 ELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLEDEQ 182
           ++  ++  V+ L +     R++A S++R ++K     R  +A  GAIP LV ++  ED  
Sbjct: 329 DMSVIRALVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVA 388

Query: 183 SQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLSAL 242
           +Q  A+  +LNL I  N NK  I+  G +  ++++++   A      E   A    LS  
Sbjct: 389 TQENAITCVLNLSIYEN-NKELIMFAGAVTSIVQVLR---AGTMEARENAAATLFSLSLA 448

Query: 243 DSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFIPF 302
           D NK +IG SGAIP LV  L+N     + + ++DA  ALFNL I   N    +    +  
Sbjct: 449 DENKIIIGGSGAIPALVDLLENG----TPRGKKDAATALFNLCIYHGNKGRAVRAGIVTA 508

Query: 303 LLNMLGDM---EVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKA 362
           L+ ML D     + +  L+ILS + + ++ + AI V  +  P L+ +L  TD    +E A
Sbjct: 509 LVKMLSDSTRHRMVDEALTILSVLANNQDAKSAI-VKANTLPALIGILQ-TDQTRNRENA 568

Query: 363 SYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLR 410
           + +L+ +  +   +  T+   G V   ++L+  G+   +++A  +LE LR
Sbjct: 569 AAILLSLCKRDTEKLITIGRLGAVVPLMDLSKNGTERGKRKAISLLELLR 608

BLAST of Carg09360 vs. ExPASy TrEMBL
Match: A0A6J1HGX9 (U-box domain-containing protein 14-like OS=Cucurbita moschata OX=3662 GN=LOC111462844 PE=4 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 4.8e-264
Identity = 502/509 (98.62%), Postives = 503/509 (98.82%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRY HRYND
Sbjct: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYGHRYND 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           EMMD EQSTGNRSASETRDLKEAEQ RN KLANGRSEKLVDLLNLAESME ENEEETRRK
Sbjct: 61  EMMDDEQSTGNRSASETRDLKEAEQPRNLKLANGRSEKLVDLLNLAESMETENEEETRRK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED
Sbjct: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS
Sbjct: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI
Sbjct: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGLVSASLELTL+GSALAQKRASRIL CLRYDKGKQVSESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLLGSALAQKRASRILGCLRYDKGKQVSESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           KRANLPQDFAPSEHFKSLTASSTSKSLPF
Sbjct: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 509

BLAST of Carg09360 vs. ExPASy TrEMBL
Match: A0A6J1K3F7 (U-box domain-containing protein 13-like OS=Cucurbita maxima OX=3661 GN=LOC111492023 PE=4 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 3.1e-263
Identity = 498/509 (97.84%), Postives = 502/509 (98.62%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND
Sbjct: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           EMMD EQSTGNRSAS++RDLKEAEQ RNPKLANGRSEKLVDLLNLAESME ENEEETRRK
Sbjct: 61  EMMDDEQSTGNRSASDSRDLKEAEQPRNPKLANGRSEKLVDLLNLAESMETENEEETRRK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EEELEELKRTVEDLQ EDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED
Sbjct: 121 EEELEELKRTVEDLQVEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           E SQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS
Sbjct: 181 EHSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIA SNISIILETDFI
Sbjct: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAVSNISIILETDFI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGLVS+SLELTL+GSALAQKRASRILECLRYDKGKQVSESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLVSSSLELTLLGSALAQKRASRILECLRYDKGKQVSESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGNLGVA VSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNLGVAAVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           KRANLPQDFAPSEHFKSLTASSTSKSLPF
Sbjct: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 509

BLAST of Carg09360 vs. ExPASy TrEMBL
Match: A0A1S3BBS8 (U-box domain-containing protein 12-like OS=Cucumis melo OX=3656 GN=LOC103488345 PE=4 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.4e-226
Identity = 443/511 (86.69%), Postives = 467/511 (91.39%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKC SN++GS+ + GI+GANA TRHFRFCT+FSGAAFRRRIYDAVSCGGSSRYRHRY D
Sbjct: 1   MAKCHSNTIGSVAVHGISGANAPTRHFRFCTSFSGAAFRRRIYDAVSCGGSSRYRHRYKD 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQ--TRNPKLANGRSEKLVDLLNLAESMEAENEEETR 120
           E+MD + + GN S S  RDLKE E+   R  KL NG+SEKLVDLLNLA+S+E ENE ETR
Sbjct: 61  EIMDEDPTMGNGSVS--RDLKEQEEEKPRKQKLGNGKSEKLVDLLNLADSVELENEAETR 120

Query: 121 RKEEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDL 180
           RKEEEL+ELK TV+DLQAEDL +RKSAAS VRLMAKEDLVIRGTLALLGAIPPLVAM+DL
Sbjct: 121 RKEEELKELKHTVKDLQAEDLGKRKSAASSVRLMAKEDLVIRGTLALLGAIPPLVAMLDL 180

Query: 181 EDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLG 240
           EDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIK E+ SNS VTEAIIANFLG
Sbjct: 181 EDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKLETTSNSSVTEAIIANFLG 240

Query: 241 LSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETD 300
           LSALDSNK VIGSSGAIPFLVKSLQ+T  KIS+QARQDALRALFNLSIA+SNI IILETD
Sbjct: 241 LSALDSNKAVIGSSGAIPFLVKSLQSTHCKISDQARQDALRALFNLSIASSNIPIILETD 300

Query: 301 FIPFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEK 360
            IPFLLNMLGDMEVSERILSILSNVVST EGRRAIS+VPDAFPILVDVLNWTDSPGCQEK
Sbjct: 301 LIPFLLNMLGDMEVSERILSILSNVVSTPEGRRAISIVPDAFPILVDVLNWTDSPGCQEK 360

Query: 361 ASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSE 420
            SYVLMVMAHKLYGERQTMVEAGLVSASLELTL+GSALAQKRASRILECLRYDKGKQVSE
Sbjct: 361 GSYVLMVMAHKLYGERQTMVEAGLVSASLELTLLGSALAQKRASRILECLRYDKGKQVSE 420

Query: 421 SFGGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRK 480
           SFGGNLG A VSAPIIGT  SSNCN DSK   EESEEAMS+EKKAVKQLVQQSLQYNMRK
Sbjct: 421 SFGGNLGGAAVSAPIIGT--SSNCNIDSKICVEESEEAMSMEKKAVKQLVQQSLQYNMRK 480

Query: 481 IVKRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           IVKRANLPQDF PSEHFKSLT SSTSKSLPF
Sbjct: 481 IVKRANLPQDFVPSEHFKSLTTSSTSKSLPF 507

BLAST of Carg09360 vs. ExPASy TrEMBL
Match: A0A0A0LPR4 (Ubiquitin-protein ligase OS=Cucumis sativus OX=3659 GN=Csa_2G382620 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.2e-225
Identity = 441/511 (86.30%), Postives = 467/511 (91.39%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKC SN++GS+ + GI+GANA TRHFRFCT+FSGAAFRRRIYDAVSCGGSSRYRHRY D
Sbjct: 1   MAKCHSNTIGSVALHGISGANAPTRHFRFCTSFSGAAFRRRIYDAVSCGGSSRYRHRYKD 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQ--TRNPKLANGRSEKLVDLLNLAESMEAENEEETR 120
           E+MD + + G+ S S  RDLKE E+   R  KL NG+SEKLVDLLNLA+S+E ENE ETR
Sbjct: 61  EIMDEDPTMGDGSVS--RDLKEPEEEKPRKQKLGNGKSEKLVDLLNLADSVELENEAETR 120

Query: 121 RKEEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDL 180
           RKE+EL+ELKRTV+DLQAEDL ++KSAAS VRLMAKEDLVIRGTLALLGAIPPLVAM+DL
Sbjct: 121 RKEDELKELKRTVKDLQAEDLGKQKSAASSVRLMAKEDLVIRGTLALLGAIPPLVAMLDL 180

Query: 181 EDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLG 240
           EDE+SQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIK E+ SNS V EAIIANFLG
Sbjct: 181 EDEESQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKLEATSNSSVAEAIIANFLG 240

Query: 241 LSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETD 300
           LSALDSNK VIGSSGAIPFLVKSLQNT  KISNQARQDALRALFNLSIA+SNI IILETD
Sbjct: 241 LSALDSNKGVIGSSGAIPFLVKSLQNTHCKISNQARQDALRALFNLSIASSNIPIILETD 300

Query: 301 FIPFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEK 360
            IPFLLNMLGDMEVSERILSILSNVVST EGRRAIS+VPDAFPILVDVLNWTDSPGCQEK
Sbjct: 301 LIPFLLNMLGDMEVSERILSILSNVVSTPEGRRAISIVPDAFPILVDVLNWTDSPGCQEK 360

Query: 361 ASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSE 420
            SYVLMVMAHKLYGERQTMVEAGLVSASLELTL+GSALAQKRASRILECLRYDKGKQVSE
Sbjct: 361 GSYVLMVMAHKLYGERQTMVEAGLVSASLELTLLGSALAQKRASRILECLRYDKGKQVSE 420

Query: 421 SFGGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRK 480
           SFGGNLG A VSAPIIGTSSSSNCN   K   EESEEAMS+EKKAVKQLVQQSLQYNMRK
Sbjct: 421 SFGGNLGGAAVSAPIIGTSSSSNCN---KICVEESEEAMSMEKKAVKQLVQQSLQYNMRK 480

Query: 481 IVKRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           IVKRANLPQDF PSEHFKSLT SSTSKSLPF
Sbjct: 481 IVKRANLPQDFVPSEHFKSLTTSSTSKSLPF 506

BLAST of Carg09360 vs. ExPASy TrEMBL
Match: A0A6J1GHF1 (U-box domain-containing protein 12-like OS=Cucurbita moschata OX=3662 GN=LOC111453991 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.2e-222
Identity = 434/509 (85.27%), Postives = 458/509 (89.98%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKC+SNS+GS+ ++GIAGANA TR+FR CTAFSGAAFRRRIYD VSCGGSSRYRHRY D
Sbjct: 1   MAKCESNSVGSVAVEGIAGANAATRYFRLCTAFSGAAFRRRIYDTVSCGGSSRYRHRYKD 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           + MD E  TG        DLKE +Q RN K+ANGRSEKL DLLNL ES+E E+E ETR+K
Sbjct: 61  QSMDDESRTG--------DLKEEKQRRNRKVANGRSEKLADLLNLVESVEMESEVETRKK 120

Query: 121 EEELEELKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLED 180
           EE LEELKRTV+DLQ EDLV+RK+AAS VRLMAKEDLVIRGTLALLGAIPPLVAM+DLED
Sbjct: 121 EEALEELKRTVKDLQVEDLVKRKAAASNVRLMAKEDLVIRGTLALLGAIPPLVAMLDLED 180

Query: 181 EQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLS 240
           EQSQIAALYALLNLGIGNN NKAAIVKVG IHKMLKLIK ESASNS VTEAIIANFLGLS
Sbjct: 181 EQSQIAALYALLNLGIGNNPNKAAIVKVGAIHKMLKLIKPESASNSSVTEAIIANFLGLS 240

Query: 241 ALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFI 300
           ALDSNK VIGSSGAIPFLVKSLQN DH ISNQARQD LRALFNLSIAASN+SI+LETD I
Sbjct: 241 ALDSNKLVIGSSGAIPFLVKSLQNKDHNISNQARQDVLRALFNLSIAASNVSIMLETDLI 300

Query: 301 PFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKAS 360
           PFLLN LGDMEVSER LSILSNVVST EGRRA+S+VPDAFPILVDVLNWTDSPGCQEKAS
Sbjct: 301 PFLLNSLGDMEVSERNLSILSNVVSTPEGRRAVSIVPDAFPILVDVLNWTDSPGCQEKAS 360

Query: 361 YVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSESF 420
           YVLMVMAHKLYGERQTMVEAGLV ASLELTL+GSALAQKRASRILE LRYDKGKQ+SESF
Sbjct: 361 YVLMVMAHKLYGERQTMVEAGLVFASLELTLLGSALAQKRASRILEFLRYDKGKQISESF 420

Query: 421 GGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480
           GGN G A VSAPIIGTSSSSN N D++   EESEEAMSVEKKAVKQLVQQSLQYNMRKIV
Sbjct: 421 GGNHGAA-VSAPIIGTSSSSNHNFDAQICMEESEEAMSVEKKAVKQLVQQSLQYNMRKIV 480

Query: 481 KRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           KRANLP DF PSEHFKSLTASSTSKSLPF
Sbjct: 481 KRANLPHDFVPSEHFKSLTASSTSKSLPF 500

BLAST of Carg09360 vs. TAIR 10
Match: AT4G31890.1 (ARM repeat superfamily protein )

HSP 1 Score: 530.8 bits (1366), Expect = 1.2e-150
Identity = 320/532 (60.15%), Postives = 387/532 (72.74%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVID---GIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHR 60
           MAKC  N++GSL++D     + ++    HFR  + FS + FRR+I DAVSCGGSSRYRH 
Sbjct: 1   MAKCHRNNIGSLILDRAPSTSSSSTSGYHFRLWSTFSRSTFRRKIVDAVSCGGSSRYRHE 60

Query: 61  YNDEMMDGEQSTGNRSASETRDLKEAEQTRNPKLANG--------RSEKLVDLLNLAESM 120
             +E  +G   T   + S     K+A+        NG        +SEKL DLLNLA   
Sbjct: 61  LREEDDEGSYVTVT-AKSTVASSKDAKANTIGAALNGVAFEETSKKSEKLCDLLNLA--- 120

Query: 121 EAENEEETRRKEEELEELKRTVEDLQAE-----------DLVRRKSAASKVRLMAKEDLV 180
           E E + ET +KEE LE LKR V +LQ+            D  ++ +AAS+VRL+AKED  
Sbjct: 121 EVEADVETMKKEEALEVLKRVVRELQSAAAAREDNDDGGDCRKKITAASEVRLLAKEDSE 180

Query: 181 IRGTLALLGAIPPLVAMV-DLEDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKL 240
            R TLA+LGAIPPLV+M+ D     +QIA+LYALLNLGIGN+ANKAAIVK G +HKMLKL
Sbjct: 181 ARVTLAMLGAIPPLVSMIDDSRIVDAQIASLYALLNLGIGNDANKAAIVKAGAVHKMLKL 240

Query: 241 IKSESASNSLVTEAIIANFLGLSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDA 300
           I+S +  +  + EA++ANFLGLSALDSNKP+IGSSGAI FLVK+LQN D   S+QAR+DA
Sbjct: 241 IESPNTPDQEIAEAVVANFLGLSALDSNKPIIGSSGAIIFLVKTLQNLDETSSSQAREDA 300

Query: 301 LRALFNLSIAASNISIILETDFIPFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVP 360
           LRAL+NLSI   N+S ILETD I +LLN LGDMEVSERIL+ILSN+V+  EGR+AI +V 
Sbjct: 301 LRALYNLSIYQPNVSFILETDLITYLLNTLGDMEVSERILAILSNLVAVPEGRKAIGLVC 360

Query: 361 DAFPILVDVLNWTDSPGCQEKASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALA 420
           DAFP+LVDVLNWTDSPGCQEKA+Y+LM+MAHK YG+RQ M+EAG+ SA LELTL+GSALA
Sbjct: 361 DAFPVLVDVLNWTDSPGCQEKATYILMLMAHKGYGDRQVMIEAGIESALLELTLLGSALA 420

Query: 421 QKRASRILECLRYDKGKQVSESFGGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAM 480
           QKRASRILECLR DKGKQV +S G       +SAPI GT        D+    EE++  M
Sbjct: 421 QKRASRILECLRVDKGKQVLDSTG---SCGALSAPIYGT-------RDNGLDHEENDLMM 480

Query: 481 SVEKKAVKQLVQQSLQYNMRKIVKRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           S E+KAVKQLVQQSLQ NM++IVKRANLPQDF PSEHFKSL+ SSTSKSLPF
Sbjct: 481 SEERKAVKQLVQQSLQSNMKRIVKRANLPQDFVPSEHFKSLSLSSTSKSLPF 518

BLAST of Carg09360 vs. TAIR 10
Match: AT4G31890.2 (ARM repeat superfamily protein )

HSP 1 Score: 530.8 bits (1366), Expect = 1.2e-150
Identity = 320/532 (60.15%), Postives = 387/532 (72.74%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVID---GIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHR 60
           MAKC  N++GSL++D     + ++    HFR  + FS + FRR+I DAVSCGGSSRYRH 
Sbjct: 1   MAKCHRNNIGSLILDRAPSTSSSSTSGYHFRLWSTFSRSTFRRKIVDAVSCGGSSRYRHE 60

Query: 61  YNDEMMDGEQSTGNRSASETRDLKEAEQTRNPKLANG--------RSEKLVDLLNLAESM 120
             +E  +G   T   + S     K+A+        NG        +SEKL DLLNLA   
Sbjct: 61  LREEDDEGSYVTVT-AKSTVASSKDAKANTIGAALNGVAFEETSKKSEKLCDLLNLA--- 120

Query: 121 EAENEEETRRKEEELEELKRTVEDLQAE-----------DLVRRKSAASKVRLMAKEDLV 180
           E E + ET +KEE LE LKR V +LQ+            D  ++ +AAS+VRL+AKED  
Sbjct: 121 EVEADVETMKKEEALEVLKRVVRELQSAAAAREDNDDGGDCRKKITAASEVRLLAKEDSE 180

Query: 181 IRGTLALLGAIPPLVAMV-DLEDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKL 240
            R TLA+LGAIPPLV+M+ D     +QIA+LYALLNLGIGN+ANKAAIVK G +HKMLKL
Sbjct: 181 ARVTLAMLGAIPPLVSMIDDSRIVDAQIASLYALLNLGIGNDANKAAIVKAGAVHKMLKL 240

Query: 241 IKSESASNSLVTEAIIANFLGLSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDA 300
           I+S +  +  + EA++ANFLGLSALDSNKP+IGSSGAI FLVK+LQN D   S+QAR+DA
Sbjct: 241 IESPNTPDQEIAEAVVANFLGLSALDSNKPIIGSSGAIIFLVKTLQNLDETSSSQAREDA 300

Query: 301 LRALFNLSIAASNISIILETDFIPFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVP 360
           LRAL+NLSI   N+S ILETD I +LLN LGDMEVSERIL+ILSN+V+  EGR+AI +V 
Sbjct: 301 LRALYNLSIYQPNVSFILETDLITYLLNTLGDMEVSERILAILSNLVAVPEGRKAIGLVC 360

Query: 361 DAFPILVDVLNWTDSPGCQEKASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALA 420
           DAFP+LVDVLNWTDSPGCQEKA+Y+LM+MAHK YG+RQ M+EAG+ SA LELTL+GSALA
Sbjct: 361 DAFPVLVDVLNWTDSPGCQEKATYILMLMAHKGYGDRQVMIEAGIESALLELTLLGSALA 420

Query: 421 QKRASRILECLRYDKGKQVSESFGGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAM 480
           QKRASRILECLR DKGKQV +S G       +SAPI GT        D+    EE++  M
Sbjct: 421 QKRASRILECLRVDKGKQVLDSTG---SCGALSAPIYGT-------RDNGLDHEENDLMM 480

Query: 481 SVEKKAVKQLVQQSLQYNMRKIVKRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           S E+KAVKQLVQQSLQ NM++IVKRANLPQDF PSEHFKSL+ SSTSKSLPF
Sbjct: 481 SEERKAVKQLVQQSLQSNMKRIVKRANLPQDFVPSEHFKSLSLSSTSKSLPF 518

BLAST of Carg09360 vs. TAIR 10
Match: AT2G25130.1 (ARM repeat superfamily protein )

HSP 1 Score: 458.0 bits (1177), Expect = 1.0e-128
Identity = 294/510 (57.65%), Postives = 358/510 (70.20%), Query Frame = 0

Query: 1   MAKCQSNSLGSLVIDGIAGANAGTRHFRFCTAFSGAAFRRRIYDAVSCGGSSRYRHRYND 60
           MAKC  N++  L++  I  A++ +        FSG++ RR I+DA+SCGGSSRYR    +
Sbjct: 1   MAKCHRNNVDPLILHRIPSASSSSLS---GNTFSGSSLRRIIFDAISCGGSSRYRRELRE 60

Query: 61  EMMDGEQSTGNRSASETRDLKEAEQTRNPKLANGRSEKLVDLLNLAESMEAENEEETRRK 120
           E  +G         S++  + E +  R P       EKL DLLNLA     E+  ET++K
Sbjct: 61  EDDEG--------CSKSTIIGE-DSMRKP-------EKLSDLLNLA---VIESGVETKKK 120

Query: 121 EEELEELKRTVEDLQAE-----DLVRRK-SAASKVRLMAKEDLVIRGTLALLGAIPPLVA 180
           EE LE LKR V+DLQAE     +   +K +AAS+VRL+AK+D+  R TLA+LGAIPPLV+
Sbjct: 121 EETLEILKRVVKDLQAEAEAEAEAAEKKIAAASEVRLLAKDDIEARVTLAMLGAIPPLVS 180

Query: 181 MVDLE--DEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAI 240
           M+D E   E + IA+LYALLNLGIGN+ NKAAIVK GV+HKMLKL++S    N  + EAI
Sbjct: 181 MIDDESQSEDALIASLYALLNLGIGNDVNKAAIVKAGVVHKMLKLVESSKPPNQAIAEAI 240

Query: 241 IANFLGLSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNIS 300
           +ANFLGLSALDSNKP+IGSSGAI FLVK+L+N +   S+QAR+DALRAL+NLSI   N+S
Sbjct: 241 VANFLGLSALDSNKPIIGSSGAIIFLVKTLKNFEETSSSQAREDALRALYNLSIYHQNVS 300

Query: 301 IILETDFIPFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDS 360
            ILETD IPFLLN LGDMEVSERIL+IL+NVVS  EGR+AI  V +AFPILVDVLNW DS
Sbjct: 301 FILETDLIPFLLNTLGDMEVSERILAILTNVVSVPEGRKAIGEVVEAFPILVDVLNWNDS 360

Query: 361 PGCQEKASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLR-YD 420
             CQEKA Y+LM+MAHK YG+R  M+EAG+ S+ LELTL+GS LAQKRASR+LECLR  D
Sbjct: 361 IKCQEKAVYILMLMAHKGYGDRNAMIEAGIESSLLELTLVGSPLAQKRASRVLECLRVVD 420

Query: 421 KGKQVSESFGGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQS 480
           KGKQ             VSAPI GTSS         R        M+ E+KAVKQLVQQS
Sbjct: 421 KGKQ-------------VSAPIYGTSSLGRERGHDLR--------MTDERKAVKQLVQQS 467

Query: 481 LQYNMRKIVKRANLPQDF-APSEHF-KSLT 500
           LQ NM++IVKRANLP DF   S+HF KSLT
Sbjct: 481 LQSNMKRIVKRANLPHDFVTTSQHFSKSLT 467

BLAST of Carg09360 vs. TAIR 10
Match: AT2G27430.1 (ARM repeat superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 9.0e-37
Identity = 124/391 (31.71%), Postives = 207/391 (52.94%), Query Frame = 0

Query: 121 EEELEE--LKRTVEDLQAEDLVRRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDL 180
           E+E EE  L++TV+ +       ++ AA ++  +A+ED   R  +A LG I  LV+MV  
Sbjct: 71  EDENEEVVLQKTVKKIHFGSWEEKEKAAIEIEKLAREDKKTRKLMAELGVIQILVSMVAS 130

Query: 181 EDEQSQIAALYALLNLGIGNNANKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLG 240
           +    Q AA+ AL+ L  G   NKA +V   +  K+ K ++    S      A     L 
Sbjct: 131 DVSGHQKAAVNALIQLSHGTYTNKALMVNADICSKLPKNVEVLDQS---TRHAFAELLLS 190

Query: 241 LSALDSNKPVIGSSGAIPFLVKSLQNTDHKISNQARQDALRALFNLSIAASNISIILETD 300
           LS+L + +  + SS  +PFL+ ++ N+D     + ++  L  + NL +   N   ++   
Sbjct: 191 LSSLTNTQLPVASSQILPFLMDTM-NSD-STDMKTKEICLATISNLCLVLENAGPLVLNG 250

Query: 301 FIPFLLNMLGDMEVSERILSILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEK 360
            +  LL+++   ++SE+ L+ L  +V T+ G++A+         L+++L W D P CQE 
Sbjct: 251 AVETLLSLMSTKDLSEKALASLGKLVVTQMGKKAMEDCLLVSKGLIEILTWEDIPKCQEY 310

Query: 361 ASYVLMVMAHKLYGERQTMVEAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVSE 420
           A+Y+LMV+AH+ + +R+ M +AG+V   LE++L+GS L QKRA ++L+  + ++      
Sbjct: 311 AAYILMVLAHQSWSQREKMAKAGIVPVLLEVSLLGSPLVQKRAVKLLQWFKDER------ 370

Query: 421 SFGGNLGVAPVSAPIIGTSSSSNCNSDSKRYPEESEEAMSVEKKAVKQLVQQSLQYNMRK 480
               N+ + P S P  G  S    +  S R  EE        +K +K LV+QSL  NM  
Sbjct: 371 ----NVRMGPHSGPQTGWVSPGMGSPMSPRSGEEG-------RKMMKNLVKQSLYKNMEM 430

Query: 481 IVKRANLPQDFAPSEHFKSLTASSTSKSLPF 510
           I +R NL  + + S   KSL  S++SKSL +
Sbjct: 431 ITRRGNLDME-SESCRLKSLIISTSSKSLTY 438

BLAST of Carg09360 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 106.7 bits (265), Expect = 5.6e-23
Identity = 87/279 (31.18%), Postives = 152/279 (54.48%), Query Frame = 0

Query: 141 RRKSAASKVRLMAKEDLVIRGTLALLGAIPPLVAMVDLEDEQSQIAALYALLNLGIGNNA 200
           ++++AA ++RL+AK ++  R  +A  GAIP LV ++   D ++Q  ++ ALLNL I N  
Sbjct: 361 QQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSI-NEG 420

Query: 201 NKAAIVKVGVIHKMLKLIKSESASNSLVTEAIIANFLGLSALDSNKPVIGSSGAIPFLVK 260
           NK AIV  G I  +++++K+ S       E   A    LS +D NK  IG++GAI  L+ 
Sbjct: 421 NKGAIVDAGAITDIVEVLKNGSME---ARENAAATLFSLSVIDENKVAIGAAGAIQALIS 480

Query: 261 SLQNTDHKISNQARQDALRALFNLSIAASNISIILETDFIPFLLNMLGDM--EVSERILS 320
            L+      + + ++DA  A+FNL I   N S  ++   +  L  +L D    + +  L+
Sbjct: 481 LLEEG----TRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALA 540

Query: 321 ILSNVVSTREGRRAISVVPDAFPILVDVLNWTDSPGCQEKASYVLMVMAHKLYGERQTMV 380
           IL+ + + +EG+ AI+   ++ P+LV+++  T SP  +E A+ +L  +            
Sbjct: 541 ILAILSTNQEGKTAIAEA-ESIPVLVEIIR-TGSPRNRENAAAILWYLCIGNIERLNVAR 600

Query: 381 EAGLVSASLELTLMGSALAQKRASRILECLRYDKGKQVS 418
           E G   A  ELT  G+  A+++A+ +LE ++  +G  V+
Sbjct: 601 EVGADVALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029482.17.9e-269100.00U-box domain-containing protein 13 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022962384.11.0e-26398.62U-box domain-containing protein 14-like [Cucurbita moschata][more]
XP_023545656.12.9e-26398.04U-box domain-containing protein 14-like [Cucurbita pepo subsp. pepo][more]
XP_022996942.16.5e-26397.84U-box domain-containing protein 13-like [Cucurbita maxima][more]
KAG6598549.16.9e-25798.59U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q8VZ407.9e-2231.18U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
O221937.4e-2030.14U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q9CAG51.3e-1927.65U-box domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=PUB7 PE=2 S... [more]
Q5VRH91.7e-1930.00U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q8GUG91.4e-1829.31U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1HGX94.8e-26498.62U-box domain-containing protein 14-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K3F73.1e-26397.84U-box domain-containing protein 13-like OS=Cucurbita maxima OX=3661 GN=LOC111492... [more]
A0A1S3BBS81.4e-22686.69U-box domain-containing protein 12-like OS=Cucumis melo OX=3656 GN=LOC103488345 ... [more]
A0A0A0LPR41.2e-22586.30Ubiquitin-protein ligase OS=Cucumis sativus OX=3659 GN=Csa_2G382620 PE=4 SV=1[more]
A0A6J1GHF11.2e-22285.27U-box domain-containing protein 12-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT4G31890.11.2e-15060.15ARM repeat superfamily protein [more]
AT4G31890.21.2e-15060.15ARM repeat superfamily protein [more]
AT2G25130.11.0e-12857.65ARM repeat superfamily protein [more]
AT2G27430.19.0e-3731.71ARM repeat superfamily protein [more]
AT3G54850.15.6e-2331.18plant U-box 14 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 99..137
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..93
NoneNo IPR availablePANTHERPTHR46700:SF1ARM REPEAT SUPERFAMILY PROTEINcoord: 1..509
NoneNo IPR availablePANTHERPTHR46700ARM REPEAT SUPERFAMILY PROTEINcoord: 1..509
IPR000225ArmadilloSMARTSM00185arm_5coord: 155..196
e-value: 7.4
score: 14.2
coord: 198..241
e-value: 45.0
score: 8.2
coord: 242..286
e-value: 2.0
score: 17.4
coord: 287..325
e-value: 4.1
score: 16.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 271..420
e-value: 1.2E-16
score: 62.7
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 107..270
e-value: 6.0E-20
score: 73.7
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 131..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09360-RACarg09360-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding