Carg09309 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09309
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	thermospermine synthase ACAULIS5
Location	Carg_Chr05: 2023666 .. 2024153 (+)
RNA-Seq Expression	Carg09309
Synteny	Carg09309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGGTATTTGATTTCGCTAATTAACTTCCAAATCTCTTCGTATTTCTCCGTTCTAATTCCATCGCCGATTCATTTGTAAATCCGTTTCTTCTCTTCAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

mRNA sequence

CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Coding sequence (CDS)

ATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Protein sequence

MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKVRLSSFDFRFCSAIFCES

Homology

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: KAG7029533.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 218.0 bits (554), Expect = 3.9e-53
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
           MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKCFGKVRLSSFDFRFCSAIFCES 106
           FALNSVLQKGTSEFQDIALLDTKCFGKVRLSSFDFRFCSAIFCES
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKVRLSSFDFRFCSAIFCES 105

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_023546524.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 182.2 bits (461), Expect = 2.3e-42
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: KAG6598599.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 182.2 bits (461), Expect = 2.3e-42
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_022961616.1 (thermospermine synthase ACAULIS5 [Cucurbita moschata])

HSP 1 Score: 181.0 bits (458), Expect = 5.2e-42
Identity = 86/87 (98.85%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEK+NYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_022997196.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 180.3 bits (456), Expect = 8.9e-42
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVIN VNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 5.6e-15
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0


Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

[Close]

BLAST of Carg09309 vs. ExPASy TrEMBL
Match: A0A6J1HAV2 (thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.5e-42
Identity = 86/87 (98.85%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEK+NYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. ExPASy TrEMBL
Match: A0A6J1K6U5 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111492169 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 4.3e-42
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVIN VNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. ExPASy TrEMBL
Match: A0A5A7VGW4 (Thermospermine synthase ACAULIS5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00130 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.9e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
           MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 33  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 92

Query: 61  SFALNSVLQKGTSEFQDIALLDTKCFGK 88
           SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 93  SFALNRVLQQGTSEFQDIALLDTKRFGK 120

[Close]

BLAST of Carg09309 vs. ExPASy TrEMBL
Match: A0A5D3BNB5 (Thermospermine synthase ACAULIS5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00150 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.9e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
           MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 33  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 92

Query: 61  SFALNSVLQKGTSEFQDIALLDTKCFGK 88
           SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 93  SFALNRVLQQGTSEFQDIALLDTKRFGK 120

[Close]

BLAST of Carg09309 vs. ExPASy TrEMBL
Match: A0A1S3BCH1 (thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.9e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 88

[Close]

BLAST of Carg09309 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 4.0e-16
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0


Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

[Close]

BLAST of Carg09309 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 4.0e-16
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0


Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 22 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 68

The following BLAST results are available for this feature:

BLAST of Carg09309 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029533.1	3.9e-53	100.00	Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023546524.1	2.3e-42	100.00	thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo]	[more]
KAG6598599.1	2.3e-42	100.00	Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022961616.1	5.2e-42	98.85	thermospermine synthase ACAULIS5 [Cucurbita moschata]	[more]
XP_022997196.1	8.9e-42	98.85	thermospermine synthase ACAULIS5-like [Cucurbita maxima]	[more]

BLAST of Carg09309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	5.6e-15	76.60	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]

BLAST of Carg09309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HAV2	2.5e-42	98.85	thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 P...	[more]
A0A6J1K6U5	4.3e-42	98.85	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149216...	[more]
A0A5A7VGW4	3.9e-35	87.50	Thermospermine synthase ACAULIS5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3BNB5	3.9e-35	87.50	Thermospermine synthase ACAULIS5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BCH1	3.9e-35	87.50	thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV...	[more]

BLAST of Carg09309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	4.0e-16	76.60	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	4.0e-16	76.60	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 46..90 e-value: 8.1E-10 score: 40.6
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 47..88 e-value: 2.2E-8 score: 33.9
None	No IPR available	PANTHER	PTHR43317:SF8	BNAA02G04280D PROTEIN	coord: 28..90
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 28..90
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 46..105 score: 13.227092

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09309-RA	Carg09309-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0010487	thermospermine synthase activity