Carg09270 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg09270 vs. NCBI nr
Match: KAG7029571.1 (yugF, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 755.4 bits (1949), Expect = 2.3e-214
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MPIVWLWIEKQEKRGQRAPICPRLTEMLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPS 60
           MPIVWLWIEKQEKRGQRAPICPRLTEMLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPS
Sbjct: 1   MPIVWLWIEKQEKRGQRAPICPRLTEMLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPS 60

Query: 61  FLPKEVDNIKDQFARELATRIERLPVSFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCL 120
           FLPKEVDNIKDQFARELATRIERLPVSFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCL
Sbjct: 61  FLPKEVDNIKDQFARELATRIERLPVSFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCL 120

Query: 121 EWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVG 180
           EWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVG
Sbjct: 121 EWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVG 180

Query: 181 PSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLY 240
           PSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLY
Sbjct: 181 PSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLY 240

Query: 241 VNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIW 300
           VNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIW
Sbjct: 241 VNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIW 300

Query: 301 GEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPY 360
           GEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPY
Sbjct: 301 GEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPY 360

Query: 361 VNNA 365
           VNNA
Sbjct: 361 VNNA 364

BLAST of Carg09270 vs. NCBI nr
Match: XP_022962117.1 (uncharacterized protein LOC111462669 isoform X1 [Cucurbita moschata])

HSP 1 Score: 698.4 bits (1801), Expect = 3.4e-197
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA 365
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA 338

BLAST of Carg09270 vs. NCBI nr
Match: XP_022962121.1 (uncharacterized protein LOC111462669 isoform X3 [Cucurbita moschata])

HSP 1 Score: 684.9 bits (1766), Expect = 3.9e-193
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 332

BLAST of Carg09270 vs. NCBI nr
Match: XP_022962120.1 (uncharacterized protein LOC111462669 isoform X2 [Cucurbita moschata])

HSP 1 Score: 683.7 bits (1763), Expect = 8.6e-193
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKE+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEL 332

BLAST of Carg09270 vs. NCBI nr
Match: KAG6598635.1 (hypothetical protein SDJN03_08413, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 683.3 bits (1762), Expect = 1.1e-192
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKE 358
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKE
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKE 331

BLAST of Carg09270 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.9e-15
Identity = 61/246 (24.80%), Postives = 117/246 (47.56%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLW 168
           +V +HGF SS   +R   PLL +  ++  A+D+  +G S+  R       +    +  + 
Sbjct: 30  LVCVHGFLSSAFSFRKVIPLLRDK-YDIIALDLPPFGQSEKSRTFIYTYQNLAKLVIGIL 89

Query: 169 KSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNL----ATLPRLI 228
           +    +  VLVG S+G  +++  A+  PE   ++VL+ +S Y + +         +P   
Sbjct: 90  EHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFH 149

Query: 229 AYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYN 288
            Y   +L K   ++  +NV+    +     +D    GR       ++  T       G  
Sbjct: 150 LYIKRWLSKEGVMKNLLNVVHDKSLIDEEMID--GYGRPFQDEQIFKAMTRFIRHREGDL 209

Query: 289 VSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAK 348
              Q++K+ +  L+IWGE+D+I+  ++  RLH +LPN+ +  + + GHL   E+P  +++
Sbjct: 210 EPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELISE 269

Query: 349 LITQFV 351
            I  F+
Sbjct: 270 HIADFI 272

BLAST of Carg09270 vs. ExPASy Swiss-Prot
Match: A4JPX5 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) OX=269482 GN=mhpC PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 9.1e-12
Identity = 66/257 (25.68%), Postives = 110/257 (42.80%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEWRYTYPLLE---EAGFETWAVDILGWGFSD-LERLPPCDVTSKRVHL 168
           +VLLHG       W   +  ++    AG+    VD  GWG SD +         + RV  
Sbjct: 39  LVLLHGSGPGATGWANFHRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLA 98

Query: 169 YQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLI 228
             L    I R   LVG S+G   A+ FA++YPE V +LVL+       G      +P   
Sbjct: 99  GVLDTLGIGRAH-LVGNSMGGHSAVAFALSYPERVGKLVLMGGG--TGGPSQFVPMPT-- 158

Query: 229 AYAGVFLLKSI-------PLRLYVNVLAFTGISLSTSLDWVN----IGRLHCLLPWWEDA 288
              G+ LL+++        L+  +NV  +   +++  L        +GR   L  + +  
Sbjct: 159 --EGIKLLQALYRDPTLENLKKMLNVFVYDASTMTEELMQTRLENMLGRRDHLENFVKSL 218

Query: 289 TVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHL 348
           T +      Y    ++ ++K   L+IWG DD+ +   + +RL   +PNA +     CGH 
Sbjct: 219 TANPKQFPDY--GHRLSEIKAPALVIWGRDDRFVPMDVGLRLVWNMPNADLHVFGRCGHW 278

Query: 349 PHVEKPNFVAKLITQFV 351
              E      +++ +F+
Sbjct: 279 AQWEHAERFNRMVLEFL 286

BLAST of Carg09270 vs. ExPASy Swiss-Prot
Match: Q476M7 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=mhpC PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 9.1e-12
Identity = 70/260 (26.92%), Postives = 112/260 (43.08%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEW----RYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSK---- 168
           VV+LHG       W    R   PL+  AG+    VD  GWG SD    P  +  S+    
Sbjct: 40  VVMLHGSGPGATGWANFNRNVEPLV-AAGYRVLLVDCPGWGKSD----PVVNAGSRSELN 99

Query: 169 -RVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLAT 228
            RV    L +  I+R  +L G S+G   A+ FA+  P+ V +LVL+       GTG   +
Sbjct: 100 GRVLKGVLDELDIERVHIL-GNSMGGHSAVAFALANPQRVGKLVLMGG-----GTGG-PS 159

Query: 229 LPRLIAYAGVFLLKSI-------PLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWED 288
           L   +   G+ LL  +        L+  +NV  +   SL+  L    +  +       E+
Sbjct: 160 LYAPMPTEGIKLLNGLYREPSIENLKRMMNVFVYDASSLTDDLMQARLDNMLARRDHLEN 219

Query: 289 ATVSFMLSGGY--NVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPEC 348
              S   +     +  S++ ++   TL+IWG DD+ +   + +RL   +PNA +     C
Sbjct: 220 FVKSLAANPKQFTDYGSRLGEITAPTLVIWGRDDRFVPMDVGLRLIAGIPNAQMHIFNRC 279

Query: 349 GHLPHVEKPNFVAKLITQFV 351
           GH    E      +++  F+
Sbjct: 280 GHWAQWEHAKAFNRMVVDFL 287

BLAST of Carg09270 vs. ExPASy Swiss-Prot
Match: B2JQW2 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=mhpC PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.2e-11
Identity = 65/255 (25.49%), Postives = 106/255 (41.57%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEWRYTYPLLE---EAGFETWAVDILGWGFSD-LERLPPCDVTSKRVHL 168
           +VLLHG       W   Y  ++    AG+    VD  GWG SD +         + RV  
Sbjct: 39  LVLLHGSGPGASGWANFYRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLK 98

Query: 169 YQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLI 228
             L    I+R   LVG S+G   A+ FA++YPE V +LVL+       G      +P   
Sbjct: 99  GVLDTLGIERAH-LVGNSMGGHSAVAFALSYPERVGKLVLMGGG--TGGPSQFVPMPT-- 158

Query: 229 AYAGVFLLKSI-------PLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSF 288
              G+ LL+ +        L+  +NV  +   +++  L    +  +       E+   S 
Sbjct: 159 --EGIKLLQGLYRDPTLENLKKMLNVFVYDASTMTEELMQTRLDNMLARRDHLENFVKSL 218

Query: 289 MLSGGY--NVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPH 348
             +     +   ++ ++K   L+IWG DD+ +   + +RL   LPNA       CGH   
Sbjct: 219 TANPKQFPDYGHRLNEIKAPALVIWGRDDRFVPLDVGLRLVWGLPNAEFHVFGRCGHWAQ 278

Query: 349 VEKPNFVAKLITQFV 351
            E      +++  F+
Sbjct: 279 WEHAERFNQMLLDFL 286

BLAST of Carg09270 vs. ExPASy Swiss-Prot
Match: Q8KZP5 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Comamonas testosteroni OX=285 GN=mhpC PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 4.5e-11
Identity = 55/187 (29.41%), Postives = 88/187 (47.06%), Query Frame = 0

Query: 178 LVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGN-LATLPRLIAYAGVFLLKSIP 237
           LVG S+G A A++FA+ YPE   +L+L+       G GN L T   +     +F L + P
Sbjct: 108 LVGNSMGGAGALNFALEYPERTGKLILMGPG----GLGNSLFTAMPMEGIKLLFKLYAEP 167

Query: 238 ----LRLYVNVLAFTGISLSTSL---DWVNIGRLHCLLPWWEDATVSFMLS------GGY 297
               L+  +NV  F    ++  L    W NI R         +   +F+LS        +
Sbjct: 168 SLDTLKQMLNVFLFDQSLITDELVQGRWANIQRN-------PEHLKNFLLSSQKLPLSSW 227

Query: 298 NVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVA 351
           NVS ++ ++K KTL+ WG DD+ +     ++L   +P+A +   P CGH    E  +   
Sbjct: 228 NVSPRMGEIKAKTLVTWGRDDRFVPLDHGLKLVANMPDAQLHVFPRCGHWAQWEHADAFN 283

BLAST of Carg09270 vs. ExPASy TrEMBL
Match: A0A6J1HBV7 (uncharacterized protein LOC111462669 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462669 PE=4 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.6e-197
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA 365
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA 338

BLAST of Carg09270 vs. ExPASy TrEMBL
Match: A0A6J1HC70 (uncharacterized protein LOC111462669 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462669 PE=4 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 1.9e-193
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 332

BLAST of Carg09270 vs. ExPASy TrEMBL
Match: A0A6J1HE71 (uncharacterized protein LOC111462669 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462669 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 4.2e-193
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKE+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEL 332

BLAST of Carg09270 vs. ExPASy TrEMBL
Match: A0A6J1KAS9 (uncharacterized protein LOC111492171 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492171 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.7e-192
Identity = 329/332 (99.10%), Postives = 331/332 (99.70%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVI+NKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVIINKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSC MSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVMVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 332

BLAST of Carg09270 vs. ExPASy TrEMBL
Match: A0A6J1KD64 (uncharacterized protein LOC111492171 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492171 PE=4 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 6.0e-192
Identity = 328/332 (98.80%), Postives = 331/332 (99.70%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVI+NKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVIINKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSC MSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVMVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKE+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEL 332

BLAST of Carg09270 vs. TAIR 10
Match: AT1G13820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 471.9 bits (1213), Expect = 4.8e-133
Identity = 230/313 (73.48%), Postives = 258/313 (82.43%), Query Frame = 0

Query: 53  VILNKFPSFLPKEVDNIKDQFARELATRIERLP--VSFSDSCTMSSCVKPSIQSKECPVV 112
           V   KFP+FLP +V+ IKD FA +LA RIERLP  VSF +   MSSCV P ++++  PVV
Sbjct: 27  VTAEKFPTFLPTDVEKIKDTFALKLAARIERLPVSVSFREDSIMSSCVTPLMRNETTPVV 86

Query: 113 LLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKS 172
           LLHGFDSSCLEWRYTYPLLEEAG ETWA DILGWGFSDL++LPPCDV SKR H Y+ WKS
Sbjct: 87  LLHGFDSSCLEWRYTYPLLEEAGLETWAFDILGWGFSDLDKLPPCDVASKREHFYKFWKS 146

Query: 173 YIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVF 232
           +IKRP+VLVGPSLGAAVAID AVN+PEAV+ LVL+DASVYAEGTGNLATLP+  AYAGV+
Sbjct: 147 HIKRPVVLVGPSLGAAVAIDIAVNHPEAVESLVLMDASVYAEGTGNLATLPKAAAYAGVY 206

Query: 233 LLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYNVSSQIE 292
           LLKSIPLRLYVN + F GISL TS DW  IGRLHCL PWWEDATVSFM SGGYNV+S I+
Sbjct: 207 LLKSIPLRLYVNFICFNGISLETSWDWTKIGRLHCLYPWWEDATVSFMTSGGYNVTSLIK 266

Query: 293 KVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAKLITQFV 352
           KV QKTLI+WGEDDQIISNKLA RLH EL NA ++QI  CGHLPHVEKP  V KLI +FV
Sbjct: 267 KVSQKTLILWGEDDQIISNKLAWRLHGELSNARVKQISNCGHLPHVEKPAAVTKLIAEFV 326

Query: 353 HEDSR-KEVPYVN 363
            E  R KEV  ++
Sbjct: 327 RETCRCKEVESIS 339

BLAST of Carg09270 vs. TAIR 10
Match: AT5G39220.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 412.9 bits (1060), Expect = 2.6e-115
Identity = 195/316 (61.71%), Postives = 240/316 (75.95%), Query Frame = 0

Query: 39  APPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPVSFS----DSCTM 98
           +PP    +  +R+    + FP+FLPKE+ NIKD FAR LA RI R+PV           M
Sbjct: 9   SPPPPATRFRQRVTANGDGFPTFLPKEIQNIKDPFARALAQRIVRIPVPLQMGNFRGSVM 68

Query: 99  SSCVKPSIQ-SKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLP 158
           SSC+KP +Q   + PVVLLH FDSSCLEWR TYPLLE+A  ETWA+D+LGWGFSDLE+LP
Sbjct: 69  SSCIKPLVQLHDKSPVVLLHCFDSSCLEWRRTYPLLEQACLETWAIDVLGWGFSDLEKLP 128

Query: 159 PCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEG 218
           PCD  SKR HL++LWK+YIKRPM+LVGPSLGA VA+DF   YPEAVD+LVLI+A+ Y+EG
Sbjct: 129 PCDAASKRHHLFELWKTYIKRPMILVGPSLGATVAVDFTATYPEAVDKLVLINANAYSEG 188

Query: 219 TGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDA 278
           TG L  LP+ IAYAGV LLKS PLRL  NVLAF    LS ++DW NIGRLHC +PWWEDA
Sbjct: 189 TGRLKELPKSIAYAGVKLLKSFPLRLLANVLAFCSSPLSENIDWTNIGRLHCQMPWWEDA 248

Query: 279 TVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHL 338
            V FM+SGGYNV+S I+ +  KTL++  E+DQI+SN+L+V+L CEL NA +R++P+ GHL
Sbjct: 249 MVDFMISGGYNVASHIKHIDHKTLVVCSENDQIVSNQLSVKLLCELANAVLREVPDSGHL 308

Query: 339 PHVEKPNFVAKLITQF 350
           PHVE P  + KLI+ F
Sbjct: 309 PHVENPKHIVKLISDF 324

BLAST of Carg09270 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 8.2e-16
Identity = 68/271 (25.09%), Postives = 116/271 (42.80%), Query Frame = 0

Query: 102 IQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKR 161
           +Q +  P+VL+HGF +S   WRY  P L +  ++ +A+D+LG+G+SD + L   D     
Sbjct: 38  VQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVWT 97

Query: 162 VHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDAS--VYAEGTGNLAT 221
             +    K  +K P V+VG SLG   A+  AV  PE V  + L++++    AE       
Sbjct: 98  DQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEA 157

Query: 222 LPRLIAYAGVFLLKSIPLRLYVNVL--------AFTGISLSTSLDWVNIGRL-------- 281
              +I    V  LK I  R+ +  L            +  S  +D  N+           
Sbjct: 158 DETVITKFIVKPLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKP 217

Query: 282 ----HCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCEL 341
               +    ++   T        Y + S + K+    L++WG+ D  +    A ++    
Sbjct: 218 ATDPNAGEVYYRLMTRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFY 277

Query: 342 PNAFIRQIPECGHLPHVEKPNFVAKLITQFV 351
            N+ +  + + GH PH E P  V K +  ++
Sbjct: 278 SNSSLVHL-QAGHCPHDEVPEAVNKALLDWL 305

BLAST of Carg09270 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 8.2e-16
Identity = 68/271 (25.09%), Postives = 116/271 (42.80%), Query Frame = 0

Query: 102 IQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKR 161
           +Q +  P+VL+HGF +S   WRY  P L +  ++ +A+D+LG+G+SD + L   D     
Sbjct: 95  VQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVWT 154

Query: 162 VHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDAS--VYAEGTGNLAT 221
             +    K  +K P V+VG SLG   A+  AV  PE V  + L++++    AE       
Sbjct: 155 DQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEA 214

Query: 222 LPRLIAYAGVFLLKSIPLRLYVNVL--------AFTGISLSTSLDWVNIGRL-------- 281
              +I    V  LK I  R+ +  L            +  S  +D  N+           
Sbjct: 215 DETVITKFIVKPLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKP 274

Query: 282 ----HCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCEL 341
               +    ++   T        Y + S + K+    L++WG+ D  +    A ++    
Sbjct: 275 ATDPNAGEVYYRLMTRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFY 334

Query: 342 PNAFIRQIPECGHLPHVEKPNFVAKLITQFV 351
            N+ +  + + GH PH E P  V K +  ++
Sbjct: 335 SNSSLVHL-QAGHCPHDEVPEAVNKALLDWL 362

BLAST of Carg09270 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 4.9e-13
Identity = 75/273 (27.47%), Postives = 122/273 (44.69%), Query Frame = 0

Query: 89  SDSCTMSSCVKPSIQSKECP-VVLLHGFDSSCLEWRYTYPLLEEAG-FETWAVDILGWGF 148
           S + T   C  P   ++  P ++LLHGF ++ + W+Y   L    G F  +  D+L +G 
Sbjct: 42  SIASTAMHCWIPKSPNRSKPNLLLLHGFGANAM-WQYGEHLRAFTGRFNVYVPDLLFFGL 101

Query: 149 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 208
           S        +    R  L +L +++  + M +VG S G  V    A  +PE V++LVL  
Sbjct: 102 SSTSEPNRTESFQARC-LMRLMEAHGVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCC 161

Query: 209 ASVYAEG---TGNLATLPRLIAYAGVFLLKSIPLRL-----YVNVLAFTGISLSTSLDWV 268
           A V  E       L  +P L    G+ L+   P +L     +  V    G+      D++
Sbjct: 162 AGVCLEEKDMEDGLFKVPNLEEATGI-LIPQTPEKLKELIRFSFVKPIKGVPSFFLWDFI 221

Query: 269 NIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCE 328
           ++      +    D   S +        S + ++KQK+LIIWGE+DQI   +L  RL   
Sbjct: 222 DVMCTE-FVEEKRDLIKSILKD---RRLSDLPRIKQKSLIIWGEEDQIFPLELGYRLKRH 281

Query: 329 L-PNAFIRQIPECGHLPHVEKPNFVAKLITQFV 351
           +  +A I  I + GH  ++EK     K +  F+
Sbjct: 282 IGESAEIVVIKKAGHAVNLEKSKEFVKHLKSFL 307