Carg09169 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09169
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein MIS12 homolog
Location	Carg_Chr11: 1250355 .. 1253566 (-)
RNA-Seq Expression	Carg09169
Synteny	Carg09169

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAAGTAAGAGCGATGTGGTATTCGATTCGCTGAATCTGGATCCACAGCTCTTCATTAATGAAGCTCTCAATGTTGTTGACGATTTGGTTGGTGACGCCTCCGATTTCTATCAATCGTATGCTTCTCTCTTTCTCATCTCTTCAATTTGGATTTATAATTTTGTTCGGTTAAGTTCAGTCCTGTGAGTTCTTTTCCCCTTCAGACAAGCATCAACCGCCTTGAAGAGTGAGGGCTCGGATAGATCCCAGGATCTTATAATGGTAAGGTTTAGTCGTTAATTTCCGATTTCTTAAGTTGGTACTCTGAAGCACTACGAAATTGTTCCGGTTTTCTTTGAATTAAATTGTTTATTCTACCTAGGGAATATCGCGGGTCCGAACTCTCGCCCAATCGGGTCTGGATAAGCGATTGGCTATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGTGGGTTCTTCTACTCTCTCTGTGAAGAGGCTCCTGCTACTTTTATTTGGCGAAATTGCTCTATTATTGCTGTTTTTGTAACTGAATTCATTTCATAAGTGAATAATTGGGCATGTAAAAATGACTGTTTGCTTATGCAGCGAGTTTCCCACTTCTAATGTGTATATATGTACTGGCTTGGGAGCTTACTCAATTTTTGAAAAACTTGTATTGCTGTTGCATCATCAAGTTTAATTTAGATCTAAATCTTTTCTTTAAGTTGTCGTAGGATGAATCTCCTGCTGACACTTCAATAAGTTATGGCTGCCTCTATGATGTGGATCTGGATACAGAGCTAGATTCTTTAAGGAATAAGCTCTCGGAGCTATCGAGTGCTTCGTATGTTTATTACTTCTTGTGTTTCTGTATATCATATTTCATAAGAAAATATAATTTTCAGGTCAGGAAAGAGAATGTTGAATTGAACCAGGAATTCCAAGCTTTAGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCCAGGGTAATTAAGTTATGCTTAGAATTGATTGTTTCTTCGTGCATTCCTGACCATACATGCGCCATTATCCTATTAGTATATTCATGAAGGGGAAGACTCGAAATGAAATATACAAATAATGTGTGGAGCTGATTTATTTTATTTTTCTTGGAATAAGTTTTTATTGTCATAGCCCGCTTGGTAGATTAAATTTTTTGCAGACGCTGACTTTCTCCTCTGATTGATCATCCTCGTCTGCATAACCACGTGATACAAACTCTAATACATCCAAATAATATTTTAGTGACTTTCTCCTCTGGTTTTGTTTATGGGACTTTATTTTGCATCTAGATTTTCTCCGACTTTATTTTGCATCTAGATTTTCACAACGATCATAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAATCTCTCCTTAGCAGATGCGTTTTAAAACCGTGAGGTTGATGACGATACGTAACAGACCAAAGCGGACAATATCTGTTAGCGGTAGACTTGGACCATTACATGAGATTTCTCTTTGTTTTCTTTGGGGTTTATCCGGGGAACTCTAGTCTGTGAATAATGCAATTTCTCTGGCTGTTTTCATTTTGAAAGCCACTTGAAACACAATTTATTCATTAGCATTAAATTTCATTGTACCTAAATTTAACAGTTGGCAGGTAAAAGATGTTCATGATTTTAGGAATCAGAATTCTAACAAATATCAAATTCTTGCAGAAATGATGGGAACTGCATCGGATCTGCGAGCGGAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTATGATTTTCATTTTTCAAGCTACTTCAGCATCAATAAGCTTGTTTTAGCTTTGTGGAAGATGAATATCATCTAACATCCTATTTAATGCTTGTTTCTATTCTTCAGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCCTATTTGAACCTCTGAACTTCTTCATTATGACATCAACCTTACTGTATATGATGCTTGCTGTGGATCTTGTCACTATGAGTTCTTCCATGTTTTAACTTGATTTAGCTGTGAGATATTCTAATTCAAATTTGTATAAATATATCATTTGGACTGATGAGTCTCCTTAATAGTCTCCATAGAGAATTTGCAGATGGGCCATGAACAGGCGTTGCCTCCCCAGATAGATCCGTACATATTTTCTCATGAAATTGAATTGCAGTTCATATTATTTTGGAACAGCTGGTGTAGATATCAAAAGGAATCATTTCTGAGGCTCTTGAACAAAAGTAAGCCTAAACTACCAACTGCAAGCGTTAGTAAAAGTGGGTTTCTGATGGATTCAAAATCAGCAATCCTCCACATCCCCTCGTTGTCAGCTTTGCCCCGATGACTCCTCTCGCTCGAGTACTCCCGGAGAAGATTGCTCAAATTGTCAACTTGGAGGATAATTTGACGCTGCGCCCGTGCTATATTTTGTATCTGGAAAATGTGAATGGAAAGCACAAAAACCATACTTCTAAGTTTGAATCTTAAAACCAAAGAAGAAAAGAAGTCATATTCATCAAAATACCTCTTCTAGCAGGGGAGATTCCTTGGCCAATTGGGAGGACGACAAAGAATGAGATAGCAGCGATCCAGTTAAGGCACCATTACTCAAGCCAGATATGAAAATTGAAGTTGCCCCGGAGCCGTTGTTGCCCGCATCAGCTTGTGAAGACAAGTTCTGCTGGCTAGCAGAAACGTTTCTAGTAGAGATCTTGGAGTTCAGCTCATCAATACGAGTTGTGAACTCATCCATCCTCTCGCTGAGAGTAGAAATCTGTTCTGAAAGTTGTGTAATGGCTCCCTGAAAGAATTTTGAGATGGATCAGCAAATTAAAAAGCTGAGGACTTCCTCGACTCATTCAGCAATACTGTTAACCAAGAAACTATATGAATGTTTATCTCTTCTTTGGGTCAAAAGTCTCTACCAACACGTTCAGCCTCGTACTAATAGAGACGTTAATTTTTATCCCAAAGCTTTTGAATTCTAGCTTCGTTTTTAGCCATCATTGGACGGTTAATTAGATTCATAATCTTCCTACGTTATCCTATCTTGGAATATA

mRNA sequence

ATGGAAGGAAGTAAGAGCGATGTGGTATTCGATTCGCTGAATCTGGATCCACAGCTCTTCATTAATGAAGCTCTCAATGTTGTTGACGATTTGGTTGGTGACGCCTCCGATTTCTATCAATCACAAGCATCAACCGCCTTGAAGAGTGAGGGCTCGGATAGATCCCAGGATCTTATAATGGGAATATCGCGGGTCCGAACTCTCGCCCAATCGGGTCTGGATAAGCGATTGGCTATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGTCAGGAAAGAGAATGTTGAATTGAACCAGGAATTCCAAGCTTTAGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGATCTGCGAGCGGAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCCTATTTGAACCTCTGAACTTCTTCATTATGACATCAACCTTACTGTATATGATGCTTGCTGTGGATCTTGTCACTATGAGTTCTTCCATGTTTTAACTTGATTTAGCTGTGAGATATTCTAATTCAAATTTGTATAAATATATCATTTGGACTGATGAGTCTCCTTAATAGTCTCCATAGAGAATTTGCAGATGGGCCATGAACAGGCGTTGCCTCCCCAGATAGATCCGTACATATTTTCTCATGAAATTGAATTGCAGTTCATATTATTTTGGAACAGCTGGTGTAGATATCAAAAGGAATCATTTCTGAGGCTCTTGAACAAAAGTAAGCCTAAACTACCAACTGCAAGCGTTAGTAAAAGTGGGTTTCTGATGGATTCAAAATCAGCAATCCTCCACATCCCCTCGTTGTCAGCTTTGCCCCGATGACTCCTCTCGCTCGAGTACTCCCGGAGAAGATTGCTCAAATTGTCAACTTGGAGGATAATTTGACGCTGCGCCCGTGCTATATTTTATACCTCTTCTAGCAGGGGAGATTCCTTGGCCAATTGGGAGGACGACAAAGAATGAGATAGCAGCGATCCAGTTAAGGCACCATTACTCAAGCCAGATATGAAAATTGAAGTTGCCCCGGAGCCGTTGTTGCCCGCATCAGCTTGTGAAGACAAGTTCTGCTGGCTAGCAGAAACGTTTCTAGTAGAGATCTTGGAGTTCAGCTCATCAATACGAGTTGTGAACTCATCCATCCTCTCGCTGAGAGTAGAAATCTGTTCTGAAAGTTGTGTAATGGCTCCCTGAAAGAATTTTGAGATGGATCAGCAAATTAAAAAGCTGAGGACTTCCTCGACTCATTCAGCAATACTGTTAACCAAGAAACTATATGAATGTTTATCTCTTCTTTGGGTCAAAAGTCTCTACCAACACGTTCAGCCTCGTACTAATAGAGACGTTAATTTTTATCCCAAAGCTTTTGAATTCTAGCTTCGTTTTTAGCCATCATTGGACGGTTAATTAGATTCATAATCTTCCTACGTTATCCTATCTTGGAATATA

Coding sequence (CDS)

ATGGAAGGAAGTAAGAGCGATGTGGTATTCGATTCGCTGAATCTGGATCCACAGCTCTTCATTAATGAAGCTCTCAATGTTGTTGACGATTTGGTTGGTGACGCCTCCGATTTCTATCAATCACAAGCATCAACCGCCTTGAAGAGTGAGGGCTCGGATAGATCCCAGGATCTTATAATGGGAATATCGCGGGTCCGAACTCTCGCCCAATCGGGTCTGGATAAGCGATTGGCTATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGTCAGGAAAGAGAATGTTGAATTGAACCAGGAATTCCAAGCTTTAGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGATCTGCGAGCGGAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCCTATTTGAACCTCTGA

Protein sequence

MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL

Homology

[Close]

BLAST of Carg09169 vs. NCBI nr
Match: XP_022931725.1 (protein MIS12 homolog isoform X2 [Cucurbita moschata] >KAG6587622.1 Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021583.1 Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 406.0 bits (1042), Expect = 2.0e-109
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQEFQALERKTA 120
           GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQEFQALERKTA
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQEFQALERKTA 120

Query: 121 SSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHT 180
           SSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHT
Sbjct: 121 SSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHT 180

Query: 181 NGDISHHHKGFSNAKLDDIQEFLAYLNL 209
           NGDISHHHKGFSNAKLDDIQEFLAYLNL
Sbjct: 181 NGDISHHHKGFSNAKLDDIQEFLAYLNL 208

[Close]

BLAST of Carg09169 vs. NCBI nr
Match: XP_022931718.1 (protein MIS12 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 389.4 bits (999), Expect = 1.9e-104
Identity = 208/240 (86.67%), Postives = 208/240 (86.67%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN                    
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180
                       VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM
Sbjct: 121 TELDSLRNKLSEVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180

Query: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL 209
           MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL
Sbjct: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL 240

[Close]

BLAST of Carg09169 vs. NCBI nr
Match: XP_023530578.1 (protein MIS12 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359.8 bits (922), Expect = 1.6e-95
Identity = 193/240 (80.42%), Postives = 197/240 (82.08%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           ME SK +VVFDSLNLDP LFINEALNVVDDLVGDA DFYQSQAS ALK+EGSDRSQDLIM
Sbjct: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRTL QSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN                    
Sbjct: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180
                       VRKENVELN EF  LERKTASSNSQANYFNEALQLYEQSSVNEMF+EM
Sbjct: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180

Query: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL 209
           MGTASDLRA+IGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDI E LAYLNL
Sbjct: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYLNL 240

[Close]

BLAST of Carg09169 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 332.8 bits (852), Expect = 2.1e-87
Identity = 176/238 (73.95%), Postives = 192/238 (80.67%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN                    
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180
                       VRKENV LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQEM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL 207
           MGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDIQEFLA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Carg09169 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.5 bits (846), Expect = 1.1e-86
Identity = 175/238 (73.53%), Postives = 191/238 (80.25%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDL +
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN                    
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180
                       VRKENV LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQEM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL 207
           MGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDIQEFLA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Carg09169 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 5.9e-40
Identity = 103/241 (42.74%), Postives = 142/241 (58.92%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDL 60
           MEGSKS+ VFDS+NL+PQ+FINEA+N V+D V  A DFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN------------------ 120
             GI+RVR L  S +D RL +WE Y L  CF+VP+ F LP +                  
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEESSSVHQDGLYDLELDA 120

Query: 121 -----------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMM 180
                      V K +VEL+ E QALER + S        NEAL+LY++SS++E+F+EM 
Sbjct: 121 ELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFKEMT 180

Query: 181 GTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFS----NAKLDDIQEFLAY 207
             AS+LRA + +LK R+M+ S+   V +      + +H K FS    + KL+D+++F A 
Sbjct: 181 KMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKFQAE 235

[Close]

BLAST of Carg09169 vs. ExPASy TrEMBL
Match: A0A6J1F096 (protein MIS12 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437868 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 9.6e-110
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQEFQALERKTA 120
           GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQEFQALERKTA
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQEFQALERKTA 120

Query: 121 SSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHT 180
           SSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHT
Sbjct: 121 SSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHT 180

Query: 181 NGDISHHHKGFSNAKLDDIQEFLAYLNL 209
           NGDISHHHKGFSNAKLDDIQEFLAYLNL
Sbjct: 181 NGDISHHHKGFSNAKLDDIQEFLAYLNL 208

[Close]

BLAST of Carg09169 vs. ExPASy TrEMBL
Match: A0A6J1EZI4 (protein MIS12 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437868 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 9.3e-105
Identity = 208/240 (86.67%), Postives = 208/240 (86.67%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN                    
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180
                       VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM
Sbjct: 121 TELDSLRNKLSEVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180

Query: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL 209
           MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL
Sbjct: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL 240

[Close]

BLAST of Carg09169 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 174/238 (73.11%), Postives = 191/238 (80.25%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN                    
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180
                       VRKEN+ LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQEM
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL 207
           MGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTN DISHHHKGFSNAKLDDIQEFLA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Carg09169 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.6e-86
Identity = 172/238 (72.27%), Postives = 190/238 (79.83%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN                    
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180
                       VRKEN+ LNQE QALER+ ASSNSQ N FNEALQLYE++SVNEMFQEM
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL 207
           MGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTNGD SHHHKGFSNAKLDDIQEFLA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Carg09169 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.8e-85
Identity = 174/239 (72.80%), Postives = 191/239 (79.92%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60
           MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN-------------------- 120
           GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN                    
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 ------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQ-E 180
                       VRKEN+ LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQ E
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQAE 180

Query: 181 MMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL 207
           MMGTAS+LRA+IGKLK+R+ME+SKLT V+K+HTN DISHHHKGFSNAKLDDIQEFLA L
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADL 239

[Close]

BLAST of Carg09169 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 165.6 bits (418), Expect = 4.2e-41
Identity = 103/241 (42.74%), Postives = 142/241 (58.92%), Query Frame = 0


Query: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDL 60
           MEGSKS+ VFDS+NL+PQ+FINEA+N V+D V  A DFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN------------------ 120
             GI+RVR L  S +D RL +WE Y L  CF+VP+ F LP +                  
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEESSSVHQDGLYDLELDA 120

Query: 121 -----------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMM 180
                      V K +VEL+ E QALER + S        NEAL+LY++SS++E+F+EM 
Sbjct: 121 ELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFKEMT 180

Query: 181 GTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFS----NAKLDDIQEFLAY 207
             AS+LRA + +LK R+M+ S+   V +      + +H K FS    + KL+D+++F A 
Sbjct: 181 KMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKFQAE 235

The following BLAST results are available for this feature:

BLAST of Carg09169 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022931725.1	2.0e-109	100.00	protein MIS12 homolog isoform X2 [Cucurbita moschata] >KAG6587622.1 Protein MIS1...	[more]
XP_022931718.1	1.9e-104	86.67	protein MIS12 homolog isoform X1 [Cucurbita moschata]	[more]
XP_023530578.1	1.6e-95	80.42	protein MIS12 homolog [Cucurbita pepo subsp. pepo]	[more]
KAG6589575.1	2.1e-87	73.95	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515499.1	1.1e-86	73.53	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg09169 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	5.9e-40	42.74	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of Carg09169 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F096	9.6e-110	100.00	protein MIS12 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437868 P...	[more]
A0A6J1EZI4	9.3e-105	86.67	protein MIS12 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437868 P...	[more]
A0A6J1JME3	1.1e-86	73.11	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1E0A1	5.6e-86	72.27	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JD43	2.8e-85	72.80	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of Carg09169 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	4.2e-41	42.74	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 105..125
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 10..123 e-value: 1.8E-16 score: 60.5
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..99 coord: 101..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09169-RA	Carg09169-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus