Carg09160 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09160
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein MIZU-KUSSEI 1
LocationCarg_Chr11: 1292329 .. 1293129 (-)
RNA-Seq ExpressionCarg09160
SyntenyCarg09160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTGGAATCCTCATCCAGAAGGCACTTCCATTGGACAAGGAAGGTTAGTAATGAAGATGAAGACGATTCCACCTTCAAATCCTCAGCATCTGCCCCAGAAGAAAAGGAGGAGGAGAACGCCAAGCCCATTTCACAGGACTCACAACATCAGCAGCAGGTTGGAGCACAATTGTCAAGGAGGAAACTGCAACAGCTTGCCGTCTCTCGGTTCCGTTCGGTTCTCACTGCCCTTGGCCGCAGCCGGAACCAGCAACTCGGTCTTCGTTCTCGAGTGGTCGGAACACTGTTTGGTTCTCGCCGAGGGCATGTTCATTTTGCATTCCAGAGAGACCCCAATTCTCATCCCGCTTTCTTGATTGAGCTTGCTACACCAATCAGTGGTCTGGTGAAGGAAATGGCATCTGGACTGGTTCGAATTGCACTGGAATGCGATAAAGAGAAAGAGGAGAACAAGAAACCAGGAAGGAGGCTGCTTGAAGAGCCTATTTGGAGGACATTTTGCAATGGGAAGAAATGCGGTTTTGCTTCAAGGCGAGAATGTGGACCTAAGGAGTGGAAGATCTTGAAGGCTGTGGAGCCAATTTCCATGGGTGCTGGCGTTTTACCGGTGAACGAAGGCACCGAGGGTGGTGGCAGCGGTGGCGTGGAGGGAAAAGAGGGATCAGATGGTGAAATCATGTACATGAGAGCCAAGTTTGAGAGAATTGTCGGCTCTAGGGACTCTGAAGCTTTCTACATGATGAACCCAGATAGTAATGGTGCTCCTGAGCTGAGTGTCTATTTACTAAGGGTGTAA

mRNA sequence

ATGGCTCTGGAATCCTCATCCAGAAGGCACTTCCATTGGACAAGGAAGGTTAGTAATGAAGATGAAGACGATTCCACCTTCAAATCCTCAGCATCTGCCCCAGAAGAAAAGGAGGAGGAGAACGCCAAGCCCATTTCACAGGACTCACAACATCAGCAGCAGGTTGGAGCACAATTGTCAAGGAGGAAACTGCAACAGCTTGCCGTCTCTCGGTTCCGTTCGGTTCTCACTGCCCTTGGCCGCAGCCGGAACCAGCAACTCGGTCTTCGTTCTCGAGTGGTCGGAACACTGTTTGGTTCTCGCCGAGGGCATGTTCATTTTGCATTCCAGAGAGACCCCAATTCTCATCCCGCTTTCTTGATTGAGCTTGCTACACCAATCAGTGGTCTGGTGAAGGAAATGGCATCTGGACTGGTTCGAATTGCACTGGAATGCGATAAAGAGAAAGAGGAGAACAAGAAACCAGGAAGGAGGCTGCTTGAAGAGCCTATTTGGAGGACATTTTGCAATGGGAAGAAATGCGGTTTTGCTTCAAGGCGAGAATGTGGACCTAAGGAGTGGAAGATCTTGAAGGCTGTGGAGCCAATTTCCATGGGTGCTGGCGTTTTACCGGTGAACGAAGGCACCGAGGGTGGTGGCAGCGGTGGCGTGGAGGGAAAAGAGGGATCAGATGGTGAAATCATGTACATGAGAGCCAAGTTTGAGAGAATTGTCGGCTCTAGGGACTCTGAAGCTTTCTACATGATGAACCCAGATAGTAATGGTGCTCCTGAGCTGAGTGTCTATTTACTAAGGGTGTAA

Coding sequence (CDS)

ATGGCTCTGGAATCCTCATCCAGAAGGCACTTCCATTGGACAAGGAAGGTTAGTAATGAAGATGAAGACGATTCCACCTTCAAATCCTCAGCATCTGCCCCAGAAGAAAAGGAGGAGGAGAACGCCAAGCCCATTTCACAGGACTCACAACATCAGCAGCAGGTTGGAGCACAATTGTCAAGGAGGAAACTGCAACAGCTTGCCGTCTCTCGGTTCCGTTCGGTTCTCACTGCCCTTGGCCGCAGCCGGAACCAGCAACTCGGTCTTCGTTCTCGAGTGGTCGGAACACTGTTTGGTTCTCGCCGAGGGCATGTTCATTTTGCATTCCAGAGAGACCCCAATTCTCATCCCGCTTTCTTGATTGAGCTTGCTACACCAATCAGTGGTCTGGTGAAGGAAATGGCATCTGGACTGGTTCGAATTGCACTGGAATGCGATAAAGAGAAAGAGGAGAACAAGAAACCAGGAAGGAGGCTGCTTGAAGAGCCTATTTGGAGGACATTTTGCAATGGGAAGAAATGCGGTTTTGCTTCAAGGCGAGAATGTGGACCTAAGGAGTGGAAGATCTTGAAGGCTGTGGAGCCAATTTCCATGGGTGCTGGCGTTTTACCGGTGAACGAAGGCACCGAGGGTGGTGGCAGCGGTGGCGTGGAGGGAAAAGAGGGATCAGATGGTGAAATCATGTACATGAGAGCCAAGTTTGAGAGAATTGTCGGCTCTAGGGACTCTGAAGCTTTCTACATGATGAACCCAGATAGTAATGGTGCTCCTGAGCTGAGTGTCTATTTACTAAGGGTGTAA

Protein sequence

MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLSRRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFLIELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRRECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSVYLLRV
Homology
BLAST of Carg09160 vs. NCBI nr
Match: XP_022929348.1 (protein MIZU-KUSSEI 1 [Cucurbita moschata] >KAG7021592.1 Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 520.0 bits (1338), Expect = 1.2e-143
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL
Sbjct: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR
Sbjct: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS
Sbjct: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELSVYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 266

BLAST of Carg09160 vs. NCBI nr
Match: KAG6587633.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 516.9 bits (1330), Expect = 1.0e-142
Identity = 264/266 (99.25%), Postives = 265/266 (99.62%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALESSSRRHFHWTRKVSNEDEDDSTFKSS SAPEEKEEENAKPISQDSQHQQQVGAQLS
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSESAPEEKEEENAKPISQDSQHQQQVGAQLS 60

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL
Sbjct: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIW+TFCNGKKCGFASRR
Sbjct: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWKTFCNGKKCGFASRR 180

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS
Sbjct: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELSVYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 266

BLAST of Carg09160 vs. NCBI nr
Match: XP_023000836.1 (uncharacterized protein LOC111495163 [Cucurbita maxima])

HSP 1 Score: 511.9 bits (1317), Expect = 3.3e-141
Identity = 263/266 (98.87%), Postives = 263/266 (98.87%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALESSSRRHFHWTRKVSNEDEDDSTFKSS SAPEEKEEENAKPISQDSQHQQQVGAQLS
Sbjct: 132 MALESSSRRHFHWTRKVSNEDEDDSTFKSSESAPEEKEEENAKPISQDSQHQQQVGAQLS 191

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL
Sbjct: 192 RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 251

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR
Sbjct: 252 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 311

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKEWKILKAVEPISMGAGVLPVNEGTEG GSGG EGKEGSDGEIMYMRAKFERIVGS
Sbjct: 312 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGCGSGGGEGKEGSDGEIMYMRAKFERIVGS 371

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELSVYLLRV
Sbjct: 372 RDSEAFYMMNPDSNGAPELSVYLLRV 397

BLAST of Carg09160 vs. NCBI nr
Match: XP_023530791.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.8 bits (1301), Expect = 2.3e-139
Identity = 261/266 (98.12%), Postives = 261/266 (98.12%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALESSSRRHFHWTRKVSNEDEDDSTFKSS SAPEEKEEENAKPISQDSQHQQQVGAQLS
Sbjct: 70  MALESSSRRHFHWTRKVSNEDEDDSTFKSSESAPEEKEEENAKPISQDSQHQQQVGAQLS 129

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL
Sbjct: 130 RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 189

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR
Sbjct: 190 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 249

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGG    EGKEGSDGEIMYMRAKFERIVGS
Sbjct: 250 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGG---EGKEGSDGEIMYMRAKFERIVGS 309

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELSVYLLRV
Sbjct: 310 RDSEAFYMMNPDSNGAPELSVYLLRV 332

BLAST of Carg09160 vs. NCBI nr
Match: XP_038879125.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 456.8 bits (1174), Expect = 1.2e-124
Identity = 237/266 (89.10%), Postives = 249/266 (93.61%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALESSSRRHFHWTRKVSNEDEDDSTF SS SA E+K EENAKPISQ+ + QQQV AQL+
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFNSSESAVEQK-EENAKPISQEPEEQQQVRAQLT 60

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGR+RN +  LRSRVVGTLFGSR+GHVHFAFQRDPNSHPAFL
Sbjct: 61  RRKLQQLAVSRFRSVLTALGRNRNTRHSLRSRVVGTLFGSRQGHVHFAFQRDPNSHPAFL 120

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEK+ENKKPGRRLLEEP+WRTFCNGKKCGFASRR
Sbjct: 121 IELATPISGLVKEMASGLVRIALECDKEKDENKKPGRRLLEEPVWRTFCNGKKCGFASRR 180

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKE KILKAVEPISMGAGVLPVN+GTEG G    EG+ GSDGEIMYMRAKFERIVGS
Sbjct: 181 ECGPKELKILKAVEPISMGAGVLPVNKGTEGSGGCSGEGEGGSDGEIMYMRAKFERIVGS 240

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELS+YLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSIYLLRV 265

BLAST of Carg09160 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.3e-44
Identity = 93/183 (50.82%), Postives = 122/183 (66.67%), Query Frame = 0

Query: 89  LRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFLIELATPISGLVKEMASGLVRIALECDKE 148
           L  RV GTL+G +RGHV F+ Q +  S P  L++LA   + LVKEM+SGLVRIALEC+K 
Sbjct: 125 LGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEK- 184

Query: 149 KEENKKPGRRLLEEPIWRTFCNGKKCGFASRR--ECGPKEWKILKAVEPISMGAGVLPVN 208
                + G +L +EP W  +CNG+KCG+A  R   C   +W++L  V  +++GAGV+P  
Sbjct: 185 ---RHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTP 244

Query: 209 ---EGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSVYL 267
              +   G GSG         GE++YMR KFER+VGSRDSEAFYMMNPD NG PELS++L
Sbjct: 245 KTIDDVSGVGSG------TELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFL 297

BLAST of Carg09160 vs. ExPASy TrEMBL
Match: A0A6J1ERU8 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111435947 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 5.8e-144
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL
Sbjct: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR
Sbjct: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS
Sbjct: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELSVYLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 266

BLAST of Carg09160 vs. ExPASy TrEMBL
Match: A0A6J1KGY0 (uncharacterized protein LOC111495163 OS=Cucurbita maxima OX=3661 GN=LOC111495163 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.6e-141
Identity = 263/266 (98.87%), Postives = 263/266 (98.87%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALESSSRRHFHWTRKVSNEDEDDSTFKSS SAPEEKEEENAKPISQDSQHQQQVGAQLS
Sbjct: 132 MALESSSRRHFHWTRKVSNEDEDDSTFKSSESAPEEKEEENAKPISQDSQHQQQVGAQLS 191

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL
Sbjct: 192 RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 251

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR
Sbjct: 252 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 311

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKEWKILKAVEPISMGAGVLPVNEGTEG GSGG EGKEGSDGEIMYMRAKFERIVGS
Sbjct: 312 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGCGSGGGEGKEGSDGEIMYMRAKFERIVGS 371

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELSVYLLRV
Sbjct: 372 RDSEAFYMMNPDSNGAPELSVYLLRV 397

BLAST of Carg09160 vs. ExPASy TrEMBL
Match: A0A6J1C1C4 (protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111007096 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 5.1e-124
Identity = 240/271 (88.56%), Postives = 249/271 (91.88%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEEN-AKPISQD--SQHQQQVGA 60
           MALESSSRRHFHWTRKVSNEDEDDSTFKSS ++ EEKEE N  KPISQ+   Q QQQV A
Sbjct: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSENSAEEKEENNTTKPISQEPQEQQQQQVRA 60

Query: 61  QLSRRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHP 120
           QL+RRKLQQLAVSRFRSVLTALGR+R+ QLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHP
Sbjct: 61  QLTRRKLQQLAVSRFRSVLTALGRNRSPQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHP 120

Query: 121 AFLIELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFA 180
           AFLIELATPISGLVKEMASGLVRIALECDKEKEENKK G RLLEEP+WRTFCNGKKCGFA
Sbjct: 121 AFLIELATPISGLVKEMASGLVRIALECDKEKEENKKAGGRLLEEPVWRTFCNGKKCGFA 180

Query: 181 SRRECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGV--EGKEGSDGEIMYMRAKFE 240
           SRRECG KEWKILKAVEPISMGAGVLPVNE  E GG GG   EG+ GSDGEIMYMRAKFE
Sbjct: 181 SRRECGAKEWKILKAVEPISMGAGVLPVNEDAESGGGGGCSKEGEGGSDGEIMYMRAKFE 240

Query: 241 RIVGSRDSEAFYMMNPDSNGAPELSVYLLRV 267
           RIVGSRDSEAFYMMNPDSNGAPELS+YLLRV
Sbjct: 241 RIVGSRDSEAFYMMNPDSNGAPELSMYLLRV 271

BLAST of Carg09160 vs. ExPASy TrEMBL
Match: A0A6J1JA11 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484901 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.1e-121
Identity = 234/267 (87.64%), Postives = 247/267 (92.51%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MALE SSRRHFHWTRKVSNE+EDDSTFKSS SA EEK +ENAK I Q+ Q QQQV AQL+
Sbjct: 1   MALEFSSRRHFHWTRKVSNEEEDDSTFKSSDSAKEEK-QENAKSILQEPQEQQQVRAQLT 60

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGR+RN QLGLRSRV+GTLFGSRRGHVHFAFQRDP+SHPAFL
Sbjct: 61  RRKLQQLAVSRFRSVLTALGRNRNPQLGLRSRVIGTLFGSRRGHVHFAFQRDPSSHPAFL 120

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEK+ NKKPG RLLEEP+WRTFCNGKKCGFASRR
Sbjct: 121 IELATPISGLVKEMASGLVRIALECDKEKDVNKKPGGRLLEEPVWRTFCNGKKCGFASRR 180

Query: 181 ECGPKEWKILKAVEPISMGAGVLPV-NEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVG 240
           ECGPKE KILKAVEPISMGAGVLPV +EGTEG   G  EG+ GSDG+IMYMRAKFERIVG
Sbjct: 181 ECGPKELKILKAVEPISMGAGVLPVEDEGTEGSSGGSGEGEGGSDGDIMYMRAKFERIVG 240

Query: 241 SRDSEAFYMMNPDSNGAPELSVYLLRV 267
           SRDSEAFYMMNPDSNGAPELS+YLLRV
Sbjct: 241 SRDSEAFYMMNPDSNGAPELSIYLLRV 266

BLAST of Carg09160 vs. ExPASy TrEMBL
Match: A0A6J1E1K6 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111430002 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.1e-121
Identity = 232/266 (87.22%), Postives = 246/266 (92.48%), Query Frame = 0

Query: 1   MALESSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQLS 60
           MA+E SS+RHFHWTRKVSNE EDDSTFKSS SA EEK +ENAK I Q+ Q QQQ  AQL+
Sbjct: 1   MAMEFSSKRHFHWTRKVSNE-EDDSTFKSSDSATEEK-QENAKSILQEPQEQQQFRAQLT 60

Query: 61  RRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFL 120
           RRKLQQLAVSRFRSVLTALGR+RN QLGLRSRV+GTLFGSRRGHVHFAFQRDP+SHPAFL
Sbjct: 61  RRKLQQLAVSRFRSVLTALGRNRNPQLGLRSRVIGTLFGSRRGHVHFAFQRDPSSHPAFL 120

Query: 121 IELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRR 180
           IELATPISGLVKEMASGLVRIALECDKEK+ NKKPG RLLEEP+WRTFCNGKKCGFASRR
Sbjct: 121 IELATPISGLVKEMASGLVRIALECDKEKDVNKKPGGRLLEEPVWRTFCNGKKCGFASRR 180

Query: 181 ECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGS 240
           ECGPKE KILKAVEPISMGAGVLPV+EGTEG G G  EG+ GSDG+IMYMRAKFERIVGS
Sbjct: 181 ECGPKELKILKAVEPISMGAGVLPVDEGTEGSGGGSGEGEGGSDGDIMYMRAKFERIVGS 240

Query: 241 RDSEAFYMMNPDSNGAPELSVYLLRV 267
           RDSEAFYMMNPDSNGAPELS+YLLRV
Sbjct: 241 RDSEAFYMMNPDSNGAPELSIYLLRV 264

BLAST of Carg09160 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 307.0 bits (785), Expect = 1.5e-83
Identity = 179/292 (61.30%), Postives = 203/292 (69.52%), Query Frame = 0

Query: 1   MALESSSRRH--FHWTRKVSNEDEDDSTFKSSASAPEEKEEENAKPISQDSQHQQQVGAQ 60
           MA + +S+RH  FHWTRKV +++ DD      +S        N KP S  S     V   
Sbjct: 1   MAKDFASKRHNSFHWTRKVGSDENDD-----VSSHKPLPHHNNTKPSSSSSSSSSNVITH 60

Query: 61  LSRRKLQQLAVSRFRSVLTALGRSR--NQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSH 120
             ++KLQ  AVSR RSV+  L R+R  NQ  GL SRVVGTLFGSRRGHVHF+ Q+DPNS 
Sbjct: 61  --KKKLQSFAVSRLRSVIATLSRARPGNQNSGLGSRVVGTLFGSRRGHVHFSIQKDPNSP 120

Query: 121 PAFLIELATPISGLVKEMASGLVRIALECDKEKEE---NKKPG----------------- 180
           PAFLIELATPISGLVKEMASGLVRIALECDK KEE    +K G                 
Sbjct: 121 PAFLIELATPISGLVKEMASGLVRIALECDKGKEEEEGEEKNGTLRHGGGDKTKTTTTAA 180

Query: 181 --RRLLEEPIWRTFCNGKKCGFASRRECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGS 240
             RRL+EEP+WRT+CNGKKCGFA+RRECG KE K+LKA+E +SMGAGVLP  E   GGG 
Sbjct: 181 VSRRLVEEPMWRTYCNGKKCGFATRRECGEKEKKVLKALEMVSMGAGVLPETEEIGGGGG 240

Query: 241 GGVEGKEGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSVYLLRV 267
           GG        G+IMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELS+YLLR+
Sbjct: 241 GG--------GDIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLRI 277

BLAST of Carg09160 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 236.1 bits (601), Expect = 3.2e-62
Identity = 147/274 (53.65%), Postives = 178/274 (64.96%), Query Frame = 0

Query: 5   SSSRRHFHWTRKVSNEDEDDSTFKSSASAPEEKEEE-----NAKPI------SQDSQHQQ 64
           S S+R+F+W +K   ED+D+          EE+EEE     N K +      S D     
Sbjct: 15  SLSKRYFNWKKKKVQEDDDE----------EEEEEEDDYNHNEKKVLTRFNFSSDPTRPD 74

Query: 65  QVGAQLSRRKLQQLAVSRFRSVLTALGRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDP 124
           Q  AQ   +K ++  + + R    ALG S +   GL  RVVGTLFG+RRGHV+FA Q DP
Sbjct: 75  QFNAQQIMKKKKKKTLEKIR---YALGFSNS---GLGFRVVGTLFGNRRGHVYFAVQDDP 134

Query: 125 NSHPAFLIELATPISGLVKEMASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKK 184
              PA LI+L TP S LV+EMASGLVRIALE    K ++KK   +LLEE  WRT+CNGKK
Sbjct: 135 TRLPAVLIQLPTPTSVLVREMASGLVRIALETAAYKTDSKK---KLLEESTWRTYCNGKK 194

Query: 185 CGFASRRECGPKEWKILKAVEPISMGAGVLPVNEGTEGGGSGGVEGKEGSDGEIMYMRAK 244
           CG+A+R+ECG  EWK+LKAV PI+MGAGVLP    T      G  G E   GE+MYMRA+
Sbjct: 195 CGYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEGNGAVGSE--KGELMYMRAR 254

Query: 245 FERIVGSRDSEAFYMMNPD-SNGAPELSVYLLRV 267
           FER+VGSRDSEAFYMMNPD S+G PELSVY LRV
Sbjct: 255 FERVVGSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

BLAST of Carg09160 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 181.4 bits (459), Expect = 9.4e-46
Identity = 93/183 (50.82%), Postives = 122/183 (66.67%), Query Frame = 0

Query: 89  LRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFLIELATPISGLVKEMASGLVRIALECDKE 148
           L  RV GTL+G +RGHV F+ Q +  S P  L++LA   + LVKEM+SGLVRIALEC+K 
Sbjct: 125 LGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEK- 184

Query: 149 KEENKKPGRRLLEEPIWRTFCNGKKCGFASRR--ECGPKEWKILKAVEPISMGAGVLPVN 208
                + G +L +EP W  +CNG+KCG+A  R   C   +W++L  V  +++GAGV+P  
Sbjct: 185 ---RHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTP 244

Query: 209 ---EGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSVYL 267
              +   G GSG         GE++YMR KFER+VGSRDSEAFYMMNPD NG PELS++L
Sbjct: 245 KTIDDVSGVGSG------TELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFL 297

BLAST of Carg09160 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 165.2 bits (417), Expect = 7.0e-41
Identity = 96/257 (37.35%), Postives = 148/257 (57.59%), Query Frame = 0

Query: 25  STFKSSASAPEEKEEENAKP---ISQDSQHQQQVGAQLSRRKLQQLAVSRFRSVLTAL-- 84
           +T     S   +K     +P   + Q S   +  G++ ++   +  +V R   +++ +  
Sbjct: 14  TTMSPLGSPKSKKSTATVRPEITLEQPSGRNKTTGSKSTKLFRRVRSVFRSLPIMSPMCK 73

Query: 85  ------GRSRNQQLGLRSRVVGTLFGSRRGHVHFAFQRDPNSHPAFLIELATPISGLVKE 144
                 GR     +   +RV GTLFG R+  V+ A Q +P S P  L+ELA P   L+++
Sbjct: 74  FPVGGGGRLHENHVHGGTRVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQD 133

Query: 145 MASGLVRIALECDKEKEENKKPGRRLLEEPIWRTFCNGKKCGFASRRECGPKEWKILKAV 204
           +  GLVRIALEC+K+  E  K    +++EPIW  +CNGKK G+  +R+   ++  +++ +
Sbjct: 134 LGVGLVRIALECEKKPSEKTK----IIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQML 193

Query: 205 EPISMGAGVLPVNEGT----EGGGSGGVEGKEGSDGEIMYMRAKFERIVGSRDSEAFYMM 264
             +SMGAGVLPV+ G      GGG GG +     +G++ YMRA FER++GSRDSE +YMM
Sbjct: 194 HAVSMGAGVLPVSSGAITEQSGGGGGGQQ-----EGDLTYMRAHFERVIGSRDSETYYMM 253

Query: 265 NPDSNGAPELSVYLLRV 267
           NPD N  PELS++ +RV
Sbjct: 254 NPDGNSGPELSIFFVRV 261

BLAST of Carg09160 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 160.6 bits (405), Expect = 1.7e-39
Identity = 78/181 (43.09%), Postives = 117/181 (64.64%), Query Frame = 0

Query: 91  SRVVGTLFGSRRGHVHFAFQRDPNSHPAFLIELATPISGLVKEMASGLVRIALECDKE-- 150
           SR+ GTLFG R+G V  + Q +P   P+ ++ELA   + L KE+++G+VRIALE +K+  
Sbjct: 95  SRITGTLFGYRKGRVSLSIQENPKCLPSLVVELAMQTTTLQKELSTGMVRIALETEKQPR 154

Query: 151 -KEENKKPGRR--LLEEPIWRTFCNGKKCGFASRRECGPKEWKILKAVEPISMGAGVLPV 210
               N K  ++  +LEEP+W  +C G+K G+  +RE   ++  +++ + P+SMGAGVLP 
Sbjct: 155 ADNNNSKTEKKTDILEEPLWTMYCKGEKTGYGVKREATEEDLNVMELLRPVSMGAGVLPG 214

Query: 211 NEGTEGGGSGGVEGKEGSDGEIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSVYLLR 267
           N  +           EG DGE+ YMRA FER++GS+DSE FYM++P+ N  PELS + +R
Sbjct: 215 NSES-----------EGPDGEMAYMRAYFERVIGSKDSETFYMLSPEGNNGPELSFFFVR 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022929348.11.2e-143100.00protein MIZU-KUSSEI 1 [Cucurbita moschata] >KAG7021592.1 Protein MIZU-KUSSEI 1, ... [more]
KAG6587633.11.0e-14299.25Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023000836.13.3e-14198.87uncharacterized protein LOC111495163 [Cucurbita maxima][more]
XP_023530791.12.3e-13998.12protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo][more]
XP_038879125.11.2e-12489.10protein MIZU-KUSSEI 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O222271.3e-4450.82Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ERU85.8e-144100.00protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111435947 PE=4 SV=1[more]
A0A6J1KGY01.6e-14198.87uncharacterized protein LOC111495163 OS=Cucurbita maxima OX=3661 GN=LOC111495163... [more]
A0A6J1C1C45.1e-12488.56protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111007096 PE=4 SV=1[more]
A0A6J1JA111.1e-12187.64protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484901 PE=4 SV=1[more]
A0A6J1E1K64.1e-12187.22protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111430002 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G23100.11.5e-8361.30Protein of unknown function, DUF617 [more]
AT3G25640.13.2e-6253.65Protein of unknown function, DUF617 [more]
AT2G41660.19.4e-4650.82Protein of unknown function, DUF617 [more]
AT5G06990.17.0e-4137.35Protein of unknown function, DUF617 [more]
AT4G39610.11.7e-3943.09Protein of unknown function, DUF617 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006460Protein MIZU-KUSSEI 1-like, plantTIGRFAMTIGR01570TIGR01570coord: 93..266
e-value: 8.2E-63
score: 209.1
IPR006460Protein MIZU-KUSSEI 1-like, plantPFAMPF04759DUF617coord: 93..265
e-value: 1.1E-68
score: 230.5
IPR006460Protein MIZU-KUSSEI 1-like, plantPANTHERPTHR31696PROTEIN MIZU-KUSSEI 1coord: 4..266
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..223
NoneNo IPR availablePANTHERPTHR31696:SF23BNAC07G49860D PROTEINcoord: 4..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09160-RACarg09160-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010274 hydrotropism