Carg08916 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08916
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Transcription initiation factor IIF subunit alpha
Location	Carg_Chr02: 9447334 .. 9451461 (+)
RNA-Seq Expression	Carg08916
Synteny	Carg08916

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAGAGTACTCCGTATCAAAGTATTCTTTGACTGTTACAGTTTCGACTGCGATTTTCTCAAAATTTTCTCAAAATTTTCGTCGCTCGCTTCATCTTCTCCTCTGTGTTCTTTTCGACATCTCTCAAATTTTTCTGTCTGTAACCTTAGTCGCTTTGTTAGATCGCTTAATTACTGTAGATTGAGGAGAAATTTGCACCAATTTACCCTGCTTCTGCAAATTTACGGTAAGAGAAACTTTCCATCCTTTTGGGGTTCTTGAGATTTAGAGGGTTTTGGCTGTTATTTGGAAAAACCCTAGACTTGTTATTGGGGTTCTTTTGCGCTTTGCGCTCTATTTTGTGGTTCCGTAGACTTTTGTTGCTGAATTAGGGGGTGGGGCAATGTCATTGGATTTGCTGCTGAAGCCGTCGTGCAGTGGATGCGGATCCACGACGGAGTTGTATGGGAGCAATTGCAAGCACATGACGTTGTGCTTGAGATGTGGCAAAACTATGGCTGAGAATAAGGGCAAATGTTACGACTGTGGTGCTACTGTTACTCGCTTAATAAGAGTTCGTTCTTTACCTCGTCTTTCTTAAGGAGCATGATGATTTGAATGTGTTGAATTTGTACTCATGTGATTAGGATTTTTTTTTTTTTTTCCTTTCATTTATGACAGATTTAAGAATTTTCATAATAGCGTGGATTGAGTATTCTCTTGCCTTCTCTTTCTGAGAGTGCATGATGAATTATATTTTAGGAATACAATGTTCGAGCGAGTTCAAGTAGTGACAAGAACTACTTCATTGGTCGGTTCATGAGTGGGCTGCCAAACTTTTCGAAGAAAAGAAATGCCGAGAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAGCTTACTGATGCCCTGCGGGTACTGACTTAATTCCTATTTTGCTTCCTTTTAAGTTTGTACTTGGATAATTTTTCGTAAGCTATGATACAGGAGAGCTATTTTTCTTTAAGAAATTATAACTGTTGGGAGAACATCAGGAAAAAAAATTTGATGCTATCTTTCAAGTAAATGAAACGGGAACACACATTCTGTAACATGGATAAAGGGTCTCTTAAGAACATGGAGTCTGAGGAACACACATTCTGTAACTTGGTTCTTCTGCCACAGTAAAGTTCAGAAATGTTTGCTCTTTTTATTAGCTTGCACTTTTCTACTTTTCTACGACTTGCATATATGTTGGACCATGTAAGTATATATTTTGTTGTTCAATATTAATTTATTACATTGTGTTGATCATAATTCAGCTTTGACCTTGGAGATCCATTTTGTTTATTTCTTCTTGTATTTATTTGTTCTTATCTCGGACTTTAAGTTAGTTGTTCTGTGTCCTATTGATTTCTTTTTTTTAATAAATAATGTACTTATATCTTATTTGCAATCGTGTCCTATTTGACCCTGATTAATTAGGATTCCATTAACACAGGAAAAGTACAAGAATAGACCCTGGCTCTTGGAAGATGAAACTGGTCAATCTCAATTCCAAGGACATCTTGAGGGTTCACAATCAGCAACATATTACTTGCTTGTATTGCAAGGAAAGGAATTTACTGCCATTCCTGCAGGTTCTTGGTATGGTTGATCTTTGAGTTAGTTTTTGTGAGTCAAGCTGTAGTCTTTTGTCTGTTTGTCGTAGTCTTCTTCCTACATGGTTTCTAAATTTACCAATTCTTTAAGGCTTCTATATTTTCTACTAGACAATAATGCGGATGGAAAATTTCAAGCCATCCATGGTAGTAAAGGGTTGATTGACCTCAATTTACAGGTACAACTTCAACAAAGTGGCACAGTATAAGCAACTTACATTGGAGGAGGCAGAAGAGAAAATGAAGAACCGAAAGAAGACAGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCGGCATTTGGTGAAGTTGAGAAGTTTGATGACAAAGATAGTGTTGGTGCTACTGGCAGGGGACGCAGAAAACCTACTAGTGACGAGAATGAAGGTAATGTTTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAACAAAAAGGGTGGTGGTGATGATGATGATGAGGAAGGCCCTAGGGGAGGTGACCATGACTTGGATGATGATGATATCAAGAAAGGTTTATCTTTTTTCCTTTAAAGAAAAACAAAGAAATTTATTTTAGGTTAAGTGCTTTACACTGTCTTTGTCATCTCTAAAGAACGTCACTTGCAATTGGCTGATGTTTACTGAGGCCATCATATGGGCATATGGTTTAGAGTTGGGGATAGCAATAGATTAGCAAATTATTTTGTTATTATCCAAGAAGATTCAAAATTTCCCATGTTTTTTTGATAATATGTTTTGGAAGGAAGCACTTTCTTGTATCCATGCAAGTGAGATTTCAGCTATTAATTCACATGATGAGTATTGAGGCCACTGTCATTGTTCCTCTATGTTAACTTGTCTGTCCAACCCTTTTAGGAGATGATTGGGAGCATGAAGAGATTTTTACCGATGATGATGAAACTACTGCTCCAGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCAGCACCTCCAGAGATTAAACAGGTTGTCTAGCACTTAGACTATTATCATTTTTCCCCCATTTGACACATATGAGTAAGAGTTTCTCAATGTTTGATTTTCATTGAACTTTAAAATCACCTGGAGTTCTGGTTGACATCTTGTATTGTCTGAAGCTTTTCCTGAAAATATCAAGTCGAAAATATCATGTATGAGAGGCAGATGGTTTGTTTGTATGACTAAGCTTTTTAGTTCCTGTAAATTCTTTTCTCACTGTGCAGGATGAAGAAGACGAGGACGAAGAAAATGAAGGAGAGGGGGGGCTCAGCAAATCTGGGAAAGAGTTGAAGAAATTGCTTAGACGTACTGGGGGGCTTAATGATTCAGATGGAGAGGAGGAGGACGACGACGACGACGACGTAAGAACCTTTTGTGCTTCCTATGAACAGAACTTAACAGCATGATAAAAAAATTTAAACAGGGACCTATGAGCAGTCCTTTGCATGGTATCTGTGTGAATTGCACTCATACTGTCTGGATTTTGATGTTATTAAGCAGCTTTGTACTTTTATTTGCTTAGGCGGAAGAGGAGAGCGGTGGCACTCCAGTTGCGCCTTCAAAGCAGAAGGATGCAGTTAAAGAAGAAGCTGCAGCCAACAGTCCCTCAAAACCAGCTCCTTCTGGATCTGCTAAGGGAACCCCTTCTACTGCAAAGACAGCAAAGGGGAAAAGAAAAAATGGCGAGGATGTGAAACCATCCAGTGCACCTCCAAAGAAAATGAAAACAGAGACGGTATGTCGCGCACTATCCCATGGTTTAAGCTCTGAGGAAGTACTTTTCTTTGTATTAGAAGAAGTCATTCTTTGTCATGAACAAACATTTCTTGGGATGTTCAGGAATCAAAGTCGACAGATGAAGGCGTGTCTACTTCTAGAAGTACAGCCTCGAAAGGTGCAGCAGCATCAGTCAAAACAGAACCAGCATCCGCTTCTGGACCCGTTACCGAGGAAGAAATTAGGGCTGTTTTGCGACAAAGGACACCTGTAACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTACGATCTCACGAGGTACGTTATGTCTTCATGACCTGTCTTACATAAACTGTTCTCGAAACAAGTTACAGTTCGAGTGATATATCCATCGCTTCCTTGACCAATAGCTTAAAACATGAAAGGATAATATTTAGAAGCTTGGACCATAGCAATTTTGTGATCCCTTGTTAACCCATTATGCAGGACAAGAATGCTTTTGCTGAAATACTGAGGAGAATTTCAAGGATTCAAAAGACGAATGGAGGACCAAGCTATGTTGTGTTGAGAAAACCTGAAGACTAGAAGAAGTAAGGACATGTTTCATCTTTGTGGGTTATGTGGTGCTTTATTATCTATTATTATCCATGCAAACAATAGATTTGTGTAAGAGATTGGTATATGTTGAGAAAGAAACAAAGAATTCTACAGGCCCTTTTTGTAAAACTAATTGTTGGTAGTGTTTGATGAAGA

mRNA sequence

TCAGAGTACTCCGTATCAAAGTATTCTTTGACTGTTACAGTTTCGACTGCGATTTTCTCAAAATTTTCTCAAAATTTTCGTCGCTCGCTTCATCTTCTCCTCTGTGTTCTTTTCGACATCTCTCAAATTTTTCTGTCTGTAACCTTAGTCGCTTTGTTAGATCGCTTAATTACTGTAGATTGAGGAGAAATTTGCACCAATTTACCCTGCTTCTGCAAATTTACGACTTTTGTTGCTGAATTAGGGGGTGGGGCAATGTCATTGGATTTGCTGCTGAAGCCGTCGTGCAGTGGATGCGGATCCACGACGGAGTTGTATGGGAGCAATTGCAAGCACATGACGTTGTGCTTGAGATGTGGCAAAACTATGGCTGAGAATAAGGGCAAATGTTACGACTGTGGTGCTACTGTTACTCGCTTAATAAGAGAATACAATGTTCGAGCGAGTTCAAGTAGTGACAAGAACTACTTCATTGGTCGGTTCATGAGTGGGCTGCCAAACTTTTCGAAGAAAAGAAATGCCGAGAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAGCTTACTGATGCCCTGCGGGAAAAGTACAAGAATAGACCCTGGCTCTTGGAAGATGAAACTGGTCAATCTCAATTCCAAGGACATCTTGAGGGTTCACAATCAGCAACATATTACTTGCTTGTATTGCAAGGAAAGGAATTTACTGCCATTCCTGCAGGTTCTTGGTATGGTTGATCTTTGAGTTAGTTTTTGTGAGTCAAGCTGTAGTCTTTTGTCTGTTTGTCGTAGTCTTCTTCCTACATGGTTTCTAAATTTACCAATTCTTTAAGGCTTCTATATTTTCTACTAGACAATAATGCGGATGGAAAATTTCAAGCCATCCATGGTAGTAAAGGGTTGATTGACCTCAATTTACAGGTACAACTTCAACAAAGTGGCACAGTATAAGCAACTTACATTGGAGGAGGCAGAAGAGAAAATGAAGAACCGAAAGAAGACAGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCGGCATTTGGTGAAGTTGAGAAGTTTGATGACAAAGATAGTGTTGGTGCTACTGGCAGGGGACGCAGAAAACCTACTAGTGACGAGAATGAAGGTAATGTTTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAACAAAAAGGGTGGTGGTGATGATGATGATGAGGAAGGCCCTAGGGGAGGTGACCATGACTTGGATGATGATGATATCAAGAAAGGAGATGATTGGGAGCATGAAGAGATTTTTACCGATGATGATGAAACTACTGCTCCAGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCAGCACCTCCAGAGATTAAACAGGATGAAGAAGACGAGGACGAAGAAAATGAAGGAGAGGGGGGGCTCAGCAAATCTGGGAAAGAGTTGAAGAAATTGCTTAGACGTACTGGGGGGCTTAATGATTCAGATGGAGAGGAGGAGGACGACGACGACGACGACGCGGAAGAGGAGAGCGGTGGCACTCCAGTTGCGCCTTCAAAGCAGAAGGATGCAGTTAAAGAAGAAGCTGCAGCCAACAGTCCCTCAAAACCAGCTCCTTCTGGATCTGCTAAGGGAACCCCTTCTACTGCAAAGACAGCAAAGGGGAAAAGAAAAAATGGCGAGGATGTGAAACCATCCAGTGCACCTCCAAAGAAAATGAAAACAGAGACGGAATCAAAGTCGACAGATGAAGGCGTGTCTACTTCTAGAAGTACAGCCTCGAAAGGTGCAGCAGCATCAGTCAAAACAGAACCAGCATCCGCTTCTGGACCCGTTACCGAGGAAGAAATTAGGGCTGTTTTGCGACAAAGGACACCTGTAACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTACGATCTCACGAGGACAAGAATGCTTTTGCTGAAATACTGAGGAGAATTTCAAGGATTCAAAAGACGAATGGAGGACCAAGCTATGTTGTGTTGAGAAAACCTGAAGACTAGAAGAAGTAAGGACATGTTTCATCTTTGTGGGTTATGTGGTGCTTTATTATCTATTATTATCCATGCAAACAATAGATTTGTGTAAGAGATTGGTATATGTTGAGAAAGAAACAAAGAATTCTACAGGCCCTTTTTGTAAAACTAATTGTTGGTAGTGTTTGATGAAGA

Coding sequence (CDS)

ATGAAGAACCGAAAGAAGACAGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCGGCATTTGGTGAAGTTGAGAAGTTTGATGACAAAGATAGTGTTGGTGCTACTGGCAGGGGACGCAGAAAACCTACTAGTGACGAGAATGAAGGTAATGTTTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAACAAAAAGGGTGGTGGTGATGATGATGATGAGGAAGGCCCTAGGGGAGGTGACCATGACTTGGATGATGATGATATCAAGAAAGGAGATGATTGGGAGCATGAAGAGATTTTTACCGATGATGATGAAACTACTGCTCCAGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCAGCACCTCCAGAGATTAAACAGGATGAAGAAGACGAGGACGAAGAAAATGAAGGAGAGGGGGGGCTCAGCAAATCTGGGAAAGAGTTGAAGAAATTGCTTAGACGTACTGGGGGGCTTAATGATTCAGATGGAGAGGAGGAGGACGACGACGACGACGACGCGGAAGAGGAGAGCGGTGGCACTCCAGTTGCGCCTTCAAAGCAGAAGGATGCAGTTAAAGAAGAAGCTGCAGCCAACAGTCCCTCAAAACCAGCTCCTTCTGGATCTGCTAAGGGAACCCCTTCTACTGCAAAGACAGCAAAGGGGAAAAGAAAAAATGGCGAGGATGTGAAACCATCCAGTGCACCTCCAAAGAAAATGAAAACAGAGACGGAATCAAAGTCGACAGATGAAGGCGTGTCTACTTCTAGAAGTACAGCCTCGAAAGGTGCAGCAGCATCAGTCAAAACAGAACCAGCATCCGCTTCTGGACCCGTTACCGAGGAAGAAATTAGGGCTGTTTTGCGACAAAGGACACCTGTAACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTACGATCTCACGAGGACAAGAATGCTTTTGCTGAAATACTGAGGAGAATTTCAAGGATTCAAAAGACGAATGGAGGACCAAGCTATGTTGTGTTGAGAAAACCTGAAGACTAG

Protein sequence

MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDRGEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGKRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED

Homology

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BLAST of Carg08916 vs. NCBI nr
Match: KAG7036268.1 (Transcription initiation factor IIF subunit alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649.4 bits (1674), Expect = 1.8e-182
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR
Sbjct: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE
Sbjct: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD
Sbjct: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180

Query: 181 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 240
           GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK
Sbjct: 181 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 240

Query: 241 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 300
           RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE
Sbjct: 241 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 300

Query: 301 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 360
           IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 301 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 360

[Close]

BLAST of Carg08916 vs. NCBI nr
Match: XP_022931161.1 (transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 646.0 bits (1665), Expect = 2.0e-181
Identity = 358/360 (99.44%), Postives = 360/360 (100.00%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR
Sbjct: 180 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 239

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGG+HDLDDDDI+KGDDWEHEEIFTDDDE
Sbjct: 240 GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDE 299

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD
Sbjct: 300 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 359

Query: 181 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 240
           GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK
Sbjct: 360 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 419

Query: 241 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 300
           RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE
Sbjct: 420 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 479

Query: 301 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 360
           IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 480 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 539

[Close]

BLAST of Carg08916 vs. NCBI nr
Match: KAG6606328.1 (Transcription initiation factor IIF subunit alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 644.0 bits (1660), Expect = 7.5e-181
Identity = 358/360 (99.44%), Postives = 359/360 (99.72%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR
Sbjct: 206 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 265

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEG RGGDHDLDDDDIKKGDDWEHEEIFTDDDE
Sbjct: 266 GEEDEEEEAARKNRLGLNKKGGGDDDDEEGHRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 325

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD
Sbjct: 326 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 385

Query: 181 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 240
           GEEE+DDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK
Sbjct: 386 GEEENDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 445

Query: 241 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 300
           RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE
Sbjct: 446 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 505

Query: 301 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 360
           IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 506 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 565

[Close]

BLAST of Carg08916 vs. NCBI nr
Match: XP_022931162.1 (transcription initiation factor IIF subunit alpha-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 641.3 bits (1653), Expect = 4.8e-180
Identity = 358/361 (99.17%), Postives = 360/361 (99.72%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR
Sbjct: 104 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 163

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGG+HDLDDDDI+KGDDWEHEEIFTDDDE
Sbjct: 164 GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDE 223

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD
Sbjct: 224 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 283

Query: 181 GEEEDDDDDD-AEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 240
           GEEEDDDDDD AEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG
Sbjct: 284 GEEEDDDDDDEAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 343

Query: 241 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 300
           KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE
Sbjct: 344 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 403

Query: 301 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 360
           EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE
Sbjct: 404 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 463

[Close]

BLAST of Carg08916 vs. NCBI nr
Match: XP_023532767.1 (transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 632.9 bits (1631), Expect = 1.7e-177
Identity = 353/361 (97.78%), Postives = 358/361 (99.17%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSD+NEGNVSDR
Sbjct: 180 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDDNEGNVSDR 239

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GE DEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDI+KGDDWEHEEIFTDDDE
Sbjct: 240 GEGDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDE 299

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLL+RTGGLNDSD
Sbjct: 300 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLKRTGGLNDSD 359

Query: 181 GE-EEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 240
           GE ++DDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGT STAKTAKG
Sbjct: 360 GEDDDDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTSSTAKTAKG 419

Query: 241 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 300
           KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE
Sbjct: 420 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 479

Query: 301 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 360
           EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE
Sbjct: 480 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 539

[Close]

BLAST of Carg08916 vs. ExPASy Swiss-Prot
Match: Q9SU25 (Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=3702 GN=RAP74 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 6.3e-98
Identity = 234/372 (62.90%), Postives = 286/372 (76.88%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGAT---GRGRRKPT-SDENEGN 60
           MKNR+KTADGY+RWMMKAA+NGPA FGEV+  ++K+S G +   GRGR+K +  DE EGN
Sbjct: 178 MKNRRKTADGYQRWMMKAANNGPALFGEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGN 237

Query: 61  VSDRGEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFT 120
           VSDRG+EDEEEEA+RK+RLGLN+K   DDDDEEGPRGGD D+DDDDI+KGDDWEHEEIFT
Sbjct: 238 VSDRGDEDEEEEASRKSRLGLNRK-SNDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFT 297

Query: 121 DDDETTAPDREERED-LAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGG 180
           DDDE    D EERED LAPE+PAPPEIKQDE+DE+ E E EGGLSKSGKELKKLL +  G
Sbjct: 298 DDDEAVGNDPEEREDLLAPEIPAPPEIKQDEDDEENEEE-EGGLSKSGKELKKLLGKANG 357

Query: 181 LNDSDGEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAK 240
           L++SD  E+DDDD D EEE+    V  SKQK+A KEE   N+P+KPAPSG  +GTP  AK
Sbjct: 358 LDESD--EDDDDDSDDEEETNYGTVTNSKQKEAAKEEPVDNAPAKPAPSGPPRGTP-PAK 417

Query: 241 TAKGKRK--NGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPAS-- 300
            +KGKRK  +G+  KPSS+  KK+KTE + KS+ +    +  + S     +VK EPAS  
Sbjct: 418 PSKGKRKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAEPASAP 477

Query: 301 ------ASGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQK 358
                 A+GPVTE+EIRAVL ++  VTTQDLV++FKARL++ EDKNAFA ILR+IS+IQK
Sbjct: 478 ASSSSAATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQK 537

[Close]

BLAST of Carg08916 vs. ExPASy TrEMBL
Match: A0A6J1ESX1 (Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111437424 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 9.5e-182
Identity = 358/360 (99.44%), Postives = 360/360 (100.00%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR
Sbjct: 180 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 239

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGG+HDLDDDDI+KGDDWEHEEIFTDDDE
Sbjct: 240 GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDE 299

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD
Sbjct: 300 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 359

Query: 181 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 240
           GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK
Sbjct: 360 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 419

Query: 241 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 300
           RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE
Sbjct: 420 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 479

Query: 301 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 360
           IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 480 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 539

[Close]

BLAST of Carg08916 vs. ExPASy TrEMBL
Match: A0A6J1ESV2 (Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111437424 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.3e-180
Identity = 358/361 (99.17%), Postives = 360/361 (99.72%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR
Sbjct: 104 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 163

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGG+HDLDDDDI+KGDDWEHEEIFTDDDE
Sbjct: 164 GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDE 223

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD
Sbjct: 224 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 283

Query: 181 GEEEDDDDDD-AEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 240
           GEEEDDDDDD AEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG
Sbjct: 284 GEEEDDDDDDEAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 343

Query: 241 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 300
           KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE
Sbjct: 344 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 403

Query: 301 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 360
           EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE
Sbjct: 404 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 463

[Close]

BLAST of Carg08916 vs. ExPASy TrEMBL
Match: A0A6J1K1N5 (Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111491607 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 5.4e-177
Identity = 354/363 (97.52%), Postives = 358/363 (98.62%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDS+GATGRGRRKPTSDENEGNVSDR
Sbjct: 180 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSIGATGRGRRKPTSDENEGNVSDR 239

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKK-GDDWEHEEIFTDDD 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDI+  GDDWEHEEIFTDDD
Sbjct: 240 GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIESIGDDWEHEEIFTDDD 299

Query: 121 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 180
           ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRR+GGLNDS
Sbjct: 300 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRSGGLNDS 359

Query: 181 DGEEE--DDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTA 240
           DGEEE  DDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGT STAKTA
Sbjct: 360 DGEEEEDDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTSSTAKTA 419

Query: 241 KGKRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVT 300
           KGKRKNGEDVKPSSAPPKKMKTETESKSTDEG+STSRSTASKGAAASVKTEPASASGPVT
Sbjct: 420 KGKRKNGEDVKPSSAPPKKMKTETESKSTDEGMSTSRSTASKGAAASVKTEPASASGPVT 479

Query: 301 EEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRK 360
           EEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRK
Sbjct: 480 EEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRK 539

[Close]

BLAST of Carg08916 vs. ExPASy TrEMBL
Match: A0A6J1KAJ0 (Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111491607 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 5.4e-177
Identity = 354/363 (97.52%), Postives = 358/363 (98.62%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDS+GATGRGRRKPTSDENEGNVSDR
Sbjct: 104 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSIGATGRGRRKPTSDENEGNVSDR 163

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKK-GDDWEHEEIFTDDD 120
           GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDI+  GDDWEHEEIFTDDD
Sbjct: 164 GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIESIGDDWEHEEIFTDDD 223

Query: 121 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 180
           ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRR+GGLNDS
Sbjct: 224 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRSGGLNDS 283

Query: 181 DGEEE--DDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTA 240
           DGEEE  DDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGT STAKTA
Sbjct: 284 DGEEEEDDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTSSTAKTA 343

Query: 241 KGKRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVT 300
           KGKRKNGEDVKPSSAPPKKMKTETESKSTDEG+STSRSTASKGAAASVKTEPASASGPVT
Sbjct: 344 KGKRKNGEDVKPSSAPPKKMKTETESKSTDEGMSTSRSTASKGAAASVKTEPASASGPVT 403

Query: 301 EEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRK 360
           EEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRK
Sbjct: 404 EEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRK 463

[Close]

BLAST of Carg08916 vs. ExPASy TrEMBL
Match: A0A0A0LCA6 (Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_3G624050 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.4e-169
Identity = 335/360 (93.06%), Postives = 348/360 (96.67%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDR 60
           MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK++VG +GRGRRK T DENEGNVSDR
Sbjct: 180 MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDR 239

Query: 61  GEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFTDDDE 120
           GEEDEEEEAARKNRLGL+KKGGGDDDDEEGPRGGDHDLDDDDI+KGDDWEHEEIFTDDDE
Sbjct: 240 GEEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDE 299

Query: 121 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 180
           TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD
Sbjct: 300 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 359

Query: 181 GEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGK 240
           G E+DDDDDD +E++GGTPV PSKQKD VKEEAAANSPSKP PSGSAKGTPSTAKTAKGK
Sbjct: 360 G-EDDDDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGK 419

Query: 241 RKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 300
           RKNGEDVKPSSAPPKKMKTETESK+TDEGV TS+STASKGA ASVKTEP+S SGPVTEEE
Sbjct: 420 RKNGEDVKPSSAPPKKMKTETESKATDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEE 479

Query: 301 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 360
           IRAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 480 IRAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538

[Close]

BLAST of Carg08916 vs. TAIR 10
Match: AT4G12610.1 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 359.0 bits (920), Expect = 4.5e-99
Identity = 234/372 (62.90%), Postives = 286/372 (76.88%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGAT---GRGRRKPT-SDENEGN 60
           MKNR+KTADGY+RWMMKAA+NGPA FGEV+  ++K+S G +   GRGR+K +  DE EGN
Sbjct: 35  MKNRRKTADGYQRWMMKAANNGPALFGEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGN 94

Query: 61  VSDRGEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFT 120
           VSDRG+EDEEEEA+RK+RLGLN+K   DDDDEEGPRGGD D+DDDDI+KGDDWEHEEIFT
Sbjct: 95  VSDRGDEDEEEEASRKSRLGLNRK-SNDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFT 154

Query: 121 DDDETTAPDREERED-LAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGG 180
           DDDE    D EERED LAPE+PAPPEIKQDE+DE+ E E EGGLSKSGKELKKLL +  G
Sbjct: 155 DDDEAVGNDPEEREDLLAPEIPAPPEIKQDEDDEENEEE-EGGLSKSGKELKKLLGKANG 214

Query: 181 LNDSDGEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAK 240
           L++SD  E+DDDD D EEE+    V  SKQK+A KEE   N+P+KPAPSG  +GTP  AK
Sbjct: 215 LDESD--EDDDDDSDDEEETNYGTVTNSKQKEAAKEEPVDNAPAKPAPSGPPRGTP-PAK 274

Query: 241 TAKGKRK--NGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPAS-- 300
            +KGKRK  +G+  KPSS+  KK+KTE + KS+ +    +  + S     +VK EPAS  
Sbjct: 275 PSKGKRKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAEPASAP 334

Query: 301 ------ASGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQK 358
                 A+GPVTE+EIRAVL ++  VTTQDLV++FKARL++ EDKNAFA ILR+IS+IQK
Sbjct: 335 ASSSSAATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQK 394

[Close]

BLAST of Carg08916 vs. TAIR 10
Match: AT4G12610.2 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 359.0 bits (920), Expect = 4.5e-99
Identity = 234/372 (62.90%), Postives = 286/372 (76.88%), Query Frame = 0


Query: 1   MKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGAT---GRGRRKPT-SDENEGN 60
           MKNR+KTADGY+RWMMKAA+NGPA FGEV+  ++K+S G +   GRGR+K +  DE EGN
Sbjct: 35  MKNRRKTADGYQRWMMKAANNGPALFGEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGN 94

Query: 61  VSDRGEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIKKGDDWEHEEIFT 120
           VSDRG+EDEEEEA+RK+RLGLN+K   DDDDEEGPRGGD D+DDDDI+KGDDWEHEEIFT
Sbjct: 95  VSDRGDEDEEEEASRKSRLGLNRK-SNDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFT 154

Query: 121 DDDETTAPDREERED-LAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGG 180
           DDDE    D EERED LAPE+PAPPEIKQDE+DE+ E E EGGLSKSGKELKKLL +  G
Sbjct: 155 DDDEAVGNDPEEREDLLAPEIPAPPEIKQDEDDEENEEE-EGGLSKSGKELKKLLGKANG 214

Query: 181 LNDSDGEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAK 240
           L++SD  E+DDDD D EEE+    V  SKQK+A KEE   N+P+KPAPSG  +GTP  AK
Sbjct: 215 LDESD--EDDDDDSDDEEETNYGTVTNSKQKEAAKEEPVDNAPAKPAPSGPPRGTP-PAK 274

Query: 241 TAKGKRK--NGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPAS-- 300
            +KGKRK  +G+  KPSS+  KK+KTE + KS+ +    +  + S     +VK EPAS  
Sbjct: 275 PSKGKRKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAEPASAP 334

Query: 301 ------ASGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQK 358
                 A+GPVTE+EIRAVL ++  VTTQDLV++FKARL++ EDKNAFA ILR+IS+IQK
Sbjct: 335 ASSSSAATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQK 394

The following BLAST results are available for this feature:

BLAST of Carg08916 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036268.1	1.8e-182	100.00	Transcription initiation factor IIF subunit alpha [Cucurbita argyrosperma subsp....	[more]
XP_022931161.1	2.0e-181	99.44	transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita mos...	[more]
KAG6606328.1	7.5e-181	99.44	Transcription initiation factor IIF subunit alpha, partial [Cucurbita argyrosper...	[more]
XP_022931162.1	4.8e-180	99.17	transcription initiation factor IIF subunit alpha-like isoform X2 [Cucurbita mos...	[more]
XP_023532767.1	1.7e-177	97.78	transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita pep...	[more]

BLAST of Carg08916 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SU25	6.3e-98	62.90	Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Carg08916 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ESX1	9.5e-182	99.44	Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1ESV2	2.3e-180	99.17	Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1K1N5	5.4e-177	97.52	Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1KAJ0	5.4e-177	97.52	Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]
A0A0A0LCA6	5.4e-169	93.06	Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=...	[more]

BLAST of Carg08916 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G12610.1	4.5e-99	62.90	transcription activators;DNA binding;RNA polymerase II transcription factors;cat...	[more]
AT4G12610.2	4.5e-99	62.90	transcription activators;DNA binding;RNA polymerase II transcription factors;cat...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008851	Transcription initiation factor IIF, alpha subunit	PFAM	PF05793	TFIIF_alpha	coord: 1..356 e-value: 6.2E-89 score: 299.0
IPR008851	Transcription initiation factor IIF, alpha subunit	PANTHER	PTHR13011	TFIIF-ALPHA	coord: 1..357
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 278..357 e-value: 5.0E-21 score: 76.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 156..178
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 179..195
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 96..111
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 65..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 238..267
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 219..237
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 21..298
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 268..282
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..56
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 289..356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08916-RA	Carg08916-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032968	positive regulation of transcription elongation from RNA polymerase II promoter
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006413	translational initiation
cellular_component	GO:0005674	transcription factor TFIIF complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016251	RNA polymerase II general transcription initiation factor activity
molecular_function	GO:0001096	TFIIF-class transcription factor complex binding
molecular_function	GO:0003743	translation initiation factor activity