Carg08887 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08887
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase CHIP-like
Location	Carg_Chr02: 9324369 .. 9328432 (-)
RNA-Seq Expression	Carg08887
Synteny	Carg08887

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAGAAAAGAAAAAAAGAAAAGGAATTTATAATTATCCGTAGAAAATAATTAGAGGGAAATTTGTCAACTTTGGCGTCAACACATATTTTGCCGGCGAATCGCTTGAAATCACTATTCTTAAGGAAACGCCTCGATTTCTGTAATCCCATTTAACCAATTTTCTCTCTGTTCTTGATTGCCTGACCCTAAAAAAACCCCCAAAGTTTCATTCATTATTGTCGCTTTGATTCCGTCGATCGGCGATGGGGCCGGGCTTCGTGGCCTTGTCTGGCGCTGCAAAACAGGCCGAGCTTCTCCGAAAAGATGGAAACCACTATTTTCAAAAAGGTAGAATCGGAGCCGCCATTGAAGCCTACACCGAGGTTTTTCTCTATCTCTCGATTTCTTTTCGTTTCTAGTTGCTATTTTGGATTTGTTAGAATTTGCAGGTAGAACGTTTGGTTGTATCAGGATCAAATCATTTGATTATTGGATTCTGTAGTTTTATTTGTGTTCGTTAATTTCGGGATGTTCTTGTTGTTTGGACTTGAAGGCGATTACGCTATGCCCGAATGTTGCTGTGTATTTGACGAACCGCGCTTTGTGTCATCGCAAGCGGAAGTATGTACGGGCACCAACCCTAATTCCTTTCAATTTCTTCATTTTTTACATTGTTTCTCTTCATTTTTACATTGGATTAGTTTTTTCTTATGGTATATAGTGATTGGAAGAAGGTGGAGGAGGATTGCCGGAGAGCTATTCAGATTGATAGCAATTCTGTGAAGGTATTGCTTTAATGTTGCGCGTGATATCAATTCAGCCTCCCCTGCTGCCTCCAACTTATTTACAGTTTTCTTATGCATTTGTCTTATCTTGAATTCTTGATCGATGATCCTTCCTTCTCTATTTCTTTGTATATCACATTTGTTGACAATTTTTTGATGTGCAGGGACATTATATGTTAGGCCTAGCATTAATACAAAATAATGTATATCCAGAAGGAATCAAGCATTTGGAGAAGGTAACTTTTGATTTTTGTATTGTACCCTCTTTTATCCTTTTCATTTGGATACAAAACAATTGATTTCTACGTAGAGATGAAATTGAGTAGAAGATTAGATTGGAGATTCATTTGAATTATGAATTGCAGAAATAACTTTCGAAAACTTTTTATATATATATATAAGTTTAGATGTAAAATTTTGCCCTTCCCTATGAAAACTTTTGATTCCATTTTTTGCTTCATGGACTTGATTGCTCTTTGCTCTGTATATTTTTTGAACCCATGAACTTCTACACTTCAAAAAGTACAGCCATTTATGGTTGTTAAAGAAATGCTTCAAATGAGAGATGGAAAAGTCTTGAGTGGTCTTGGTCCAAATGGGAAATGGATGTCCATTGGATGAAGAACATAGCAAACCAGAAGTTATTTTGGGTCATGGTGGGTGGATTTCTATAGTTGTTTTGAAGCAATCGGTAGCCACTTTGGAGGCCTAATTAAGGTATCTTCTAAAACCACTAATATCTTGACCTGCAGCTCGATTTTCTTTGAGGTACAAAGAAATTCTTGTGGTTTCCTTCCTCATCAATCAAAATTAGAGGTAGAAAAGGAGGCAACCTTTTTCTCAGATCGGGGCGACGTTTCATAATCTGATCTGCCCACCATCTGTTTCTGCACTGTTCGAAAGGAGACTTGATTTAAGTGAGCTTTTCAAATACCTGGATTTACAACCCATCCTCATCATTCCTTCCAATCCTCAAGTTTAATACCTTGAACCCATATATTGTTCATGTAGATTTTATCGTGTAATTACCGAACCGAGTAAAGATCATGGTGCTTGCTTGGGAAAATGTTTGGCTAGAGCACCTCATTTCTCACAGCCACCCACTGAACTCCTTTCAAGTTCCTTTACAGATGAGATCCTTCTCCTGTTTTCTGCATAGCTGATTCAACTGTAGTTTGAACAGTTTAGAGAAACGACTACTTAACAGATACACAATGCTTGATTTACATGGGGGACGCCTACTGTGCAGCACAGCAGCACAGTAAAGGGATTTTTAGTTTGATAAGATGAGAGAATGTGTGCCTTGTATGTCTTTTGAGAGTTTAGTCTGTCATAGTAGGAGGCGTGGGTTGTAACAGAGAAAGTGATAGCAATTGGTTAGATAAGATGGAAGGTAGAGTACTAATTGAAAAGCAGCTGTCACTTATCTTGACATTACTTTCTTTATGTTTCAGCTAAATGAAATTTTTTTCCTAAGAATGTGCAAATCTCCTATTCACAACTAGTTCTACAAATGAATCTACAGAATTCTGAATGAAACTTCTGTGGTGTTGACTCATTTCTTCCGAGTAACGTTTCAATTCAAATAATTTTTTCAATAATGTAAAGAGATATCGGTTTTGAATCTAAAAATATTTTAGACTTTTGGTTCGTGTACGTTGCATCCCTGCCACTGAGCATGTAGTTTCTGTAAGCATGACATGTTTACTATACAAACATAAATTTAATTTCATCTCTTTTTACAGGCTTTGGATCTTGGTAGGGGTGCAAATCCAAAGAGTTATATGGTTGAGGAAATATGGCAGGAGCTTGCAAAAGCAAAATACAGGGAGTGGGAAAAAGCTTCTACCAAACGTTCATGGGAATTACAGACCTTGAAGTTAGTTATTTTTCTGAGTTCAGCCTTTTGGTTTCCAGAGTTCAATGCATGTACACTTTTGAAATCGATAGTTAGGCACACGTTTTTTAGCTGAAAATATTAATGAACCATAACCGTTAAACAATGGACTTAGGAAATGAGTTTCAAGTCTTGGTGACCATTTGCTTAGAATTTAATATCTTCCGAGTGTACCTAGCAACTAAATATTGTAGGGCCAGGCAGTCGTCACATGAGAATAGTCGAGGTCCGCACAAGTTGCATGAACACTCTTGATACTAAAAATTTGAGACGGGGGTTTCTCGATTCTGTTTCCTTTTTTTGATTTTGGTGAATGTATTGTTTTTCAGAGAAGCTTGTGAGGCAGCCCTTGAACAAAAGTATTTTCTTGACCATTCTGAACTAGAAGGGTTTGTAGATGAAGCTGAAATTGCTCATAGGAAACAATTAAAGTCTCTAAGAAGTGTGTTTAAAAAAGTTGGGGAGGCGGATGCGCCATCCGAGGTAATAAGCATGATGGTTGCTATCTTCAATTTTTCACTGTGACAAGCTTTTAGGCATAACATTTGCTGTTTAAATCCTGTGGAGGTACTAATTCAATATTCTCAGGTGCCTGATTATCTTTGTTGTAAAATTACACTTGATATTCTTCGTGATCCCGTTATTACTCCGAGCGGGGTTACATATGAGAGAGCAGTGATACTCGACCATTTTCGAAAGGTGACAACATTACACCTTCCACTGCCATTTTCCCATGTCTTGTTGCTACAATTTTAACCATTTTCTTCCTTATCTAGGTGGGTAACTTCGATCCCTTAACGCGTGAACTGCTCGATGAGTCGCAACTTGTACCGAATTTAGCCATAAAGGAAGCAGTAGAAGCCTTTCTAGATAAGCATGGATGGGCTTATAATACAGATTAAGTTGAATTTGAAACGGAACTCAATCGAGTTGATGGTTAGGCCTGCACGGTCTCGTGTCTTTAATGGCGTGAGTTTGTCCTCGACTCTTCTGTATGCATTGTCGATTATGGTTTGTCATATGATATCATGCTTTCCATGACATTCTGAGGTGGTAGCGTCAATCATGTATATGATGTCCTGTCTTGATAATTTTATTGTACAGTTATTACTTAAACCTTCATTACGGTTTTAATTGGAAGGCTATGTTCAGCTTCGGTGCGCGTTTGTTCCTTTTTGGCTAAGCCTTGTCGTGTTCAAACCTTTGATCTACTAATCGAGAGTATGTATTAACCTGCTGAGTTATGTTTAGGTTGACATACCTTTGCATATTTAAATTGACGCCGTTCTTGATGAAACCTTAACAATAATTTGTTTGTTCTTATTATGCGGAACATTTTCTTATTTTACGTAATGCTTGATTACAACTTGTAAAGCGAGGTTAGTATGAGTTTCAAAT

mRNA sequence

AAAAAAAGAAAAGAAAAAAAGAAAAGGAATTTATAATTATCCGTAGAAAATAATTAGAGGGAAATTTGTCAACTTTGGCGTCAACACATATTTTGCCGGCGAATCGCTTGAAATCACTATTCTTAAGGAAACGCCTCGATTTCTGTAATCCCATTTAACCAATTTTCTCTCTGTTCTTGATTGCCTGACCCTAAAAAAACCCCCAAAGTTTCATTCATTATTGTCGCTTTGATTCCGTCGATCGGCGATGGGGCCGGGCTTCGTGGCCTTGTCTGGCGCTGCAAAACAGGCCGAGCTTCTCCGAAAAGATGGAAACCACTATTTTCAAAAAGGTAGAATCGGAGCCGCCATTGAAGCCTACACCGAGGCGATTACGCTATGCCCGAATGTTGCTGTGTATTTGACGAACCGCGCTTTGTGTCATCGCAAGCGGAAGTATTTTTTTCTTATGGTATATAGTGATTGGAAGAAGGTGGAGGAGGATTGCCGGAGAGCTATTCAGATTGATAGCAATTCTGTGAAGGGACATTATATGTTAGGCCTAGCATTAATACAAAATAATGTATATCCAGAAGGAATCAAGCATTTGGAGAAGGCTTTGGATCTTGGTAGGGGTGCAAATCCAAAGAGTTATATGGTTGAGGAAATATGGCAGGAGCTTGCAAAAGCAAAATACAGGGAGTGGGAAAAAGCTTCTACCAAACGTTCATGGGAATTACAGACCTTGAAAGAAGCTTGTGAGGCAGCCCTTGAACAAAAGTATTTTCTTGACCATTCTGAACTAGAAGGGTTTGTAGATGAAGCTGAAATTGCTCATAGGAAACAATTAAAGTCTCTAAGAAGTGTGTTTAAAAAAGTTGGGGAGGCGGATGCGCCATCCGAGGTGCCTGATTATCTTTGTTGTAAAATTACACTTGATATTCTTCGTGATCCCGTTATTACTCCGAGCGGGGTTACATATGAGAGAGCAGTGATACTCGACCATTTTCGAAAGGTGGGTAACTTCGATCCCTTAACGCGTGAACTGCTCGATGAGTCGCAACTTGTACCGAATTTAGCCATAAAGGAAGCAGTAGAAGCCTTTCTAGATAAGCATGGATGGGCTTATAATACAGATTAAGTTGAATTTGAAACGGAACTCAATCGAGTTGATGGTTAGGCCTGCACGGTCTCGTGTCTTTAATGGCGTGAGTTTGTCCTCGACTCTTCTGTATGCATTGTCGATTATGGTTTGTCATATGATATCATGCTTTCCATGACATTCTGAGGTGGTAGCGTCAATCATGTATATGATGTCCTGTCTTGATAATTTTATTGTACAGTTATTACTTAAACCTTCATTACGGTTTTAATTGGAAGGCTATGTTCAGCTTCGGTGCGCGTTTGTTCCTTTTTGGCTAAGCCTTGTCGTGTTCAAACCTTTGATCTACTAATCGAGAGTATGTATTAACCTGCTGAGTTATGTTTAGGTTGACATACCTTTGCATATTTAAATTGACGCCGTTCTTGATGAAACCTTAACAATAATTTGTTTGTTCTTATTATGCGGAACATTTTCTTATTTTACGTAATGCTTGATTACAACTTGTAAAGCGAGGTTAGTATGAGTTTCAAAT

Coding sequence (CDS)

ATGGGGCCGGGCTTCGTGGCCTTGTCTGGCGCTGCAAAACAGGCCGAGCTTCTCCGAAAAGATGGAAACCACTATTTTCAAAAAGGTAGAATCGGAGCCGCCATTGAAGCCTACACCGAGGCGATTACGCTATGCCCGAATGTTGCTGTGTATTTGACGAACCGCGCTTTGTGTCATCGCAAGCGGAAGTATTTTTTTCTTATGGTATATAGTGATTGGAAGAAGGTGGAGGAGGATTGCCGGAGAGCTATTCAGATTGATAGCAATTCTGTGAAGGGACATTATATGTTAGGCCTAGCATTAATACAAAATAATGTATATCCAGAAGGAATCAAGCATTTGGAGAAGGCTTTGGATCTTGGTAGGGGTGCAAATCCAAAGAGTTATATGGTTGAGGAAATATGGCAGGAGCTTGCAAAAGCAAAATACAGGGAGTGGGAAAAAGCTTCTACCAAACGTTCATGGGAATTACAGACCTTGAAAGAAGCTTGTGAGGCAGCCCTTGAACAAAAGTATTTTCTTGACCATTCTGAACTAGAAGGGTTTGTAGATGAAGCTGAAATTGCTCATAGGAAACAATTAAAGTCTCTAAGAAGTGTGTTTAAAAAAGTTGGGGAGGCGGATGCGCCATCCGAGGTGCCTGATTATCTTTGTTGTAAAATTACACTTGATATTCTTCGTGATCCCGTTATTACTCCGAGCGGGGTTACATATGAGAGAGCAGTGATACTCGACCATTTTCGAAAGGTGGGTAACTTCGATCCCTTAACGCGTGAACTGCTCGATGAGTCGCAACTTGTACCGAATTTAGCCATAAAGGAAGCAGTAGAAGCCTTTCTAGATAAGCATGGATGGGCTTATAATACAGATTAA

Protein sequence

MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHRKRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD

Homology

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BLAST of Carg08887 vs. NCBI nr
Match: KAG7036240.1 (E3 ubiquitin-protein ligase CHIP [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.8 bits (1527), Expect = 1.6e-165
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL
Sbjct: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE
Sbjct: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD
Sbjct: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 290

[Close]

BLAST of Carg08887 vs. NCBI nr
Match: KAG6606301.1 (E3 ubiquitin-protein ligase CHIP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.8 bits (1462), Expect = 5.5e-158
Identity = 281/290 (96.90%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE
Sbjct: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD
Sbjct: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of Carg08887 vs. NCBI nr
Match: XP_022931210.1 (E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ubiquitin-protein ligase CHIP-like [Cucurbita maxima])

HSP 1 Score: 566.2 bits (1458), Expect = 1.6e-157
Identity = 280/290 (96.55%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE
Sbjct: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEAEIAHRKQLKSLRSVF+KVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEAEIAHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD
Sbjct: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of Carg08887 vs. NCBI nr
Match: XP_023533768.1 (E3 ubiquitin-protein ligase CHIP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.8 bits (1449), Expect = 1.8e-156
Identity = 278/290 (95.86%), Postives = 281/290 (96.90%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSYMVEEIWQELAKAKYR+WEKASTKRSWELQTLKEACEAALEQKYFLDHSELE
Sbjct: 121 GRGANPKSYMVEEIWQELAKAKYRQWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEAEIAHRKQLKSLRSVF+KVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEAEIAHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAY TD
Sbjct: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYKTD 282

[Close]

BLAST of Carg08887 vs. NCBI nr
Match: XP_038886937.1 (E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida])

HSP 1 Score: 528.1 bits (1359), Expect = 4.8e-146
Identity = 262/289 (90.66%), Postives = 272/289 (94.12%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPG VALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPG-VALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQ+DS+SVK HYMLGLALIQN  YPEGIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQLDSSSVKAHYMLGLALIQNKEYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSY+VEEIWQELAKAKYREWE+AST+RSWELQTLKEACEAALEQKYFLD SELE
Sbjct: 121 GRGANPKSYIVEEIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEAEIAHR QLKSLRSVF+KV EADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEAEIAHRNQLKSLRSVFEKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNT 290
           AVILDHF KVGNFDP+TRELLDESQLVPNLAIKEAV++FLDKHGWAYNT
Sbjct: 241 AVILDHFHKVGNFDPITRELLDESQLVPNLAIKEAVQSFLDKHGWAYNT 280

[Close]

BLAST of Carg08887 vs. ExPASy Swiss-Prot
Match: Q9SRS9 (E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.6e-99
Identity = 178/277 (64.26%), Postives = 209/277 (75.45%), Query Frame = 0


Query: 11  AAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHRKRKYFFLMVY 70
           A+  AE L++DGN+ F+K R GAAI+AYTEAI L PNV  Y TNRALCH KRK       
Sbjct: 6   ASAMAERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRK------- 65

Query: 71  SDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKSYM 130
            DW KVEEDCR+AIQ+  NSVK HYMLGLAL+Q   +  G+K L++ALDLGR +NP  YM
Sbjct: 66  -DWTKVEEDCRKAIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYM 125

Query: 131 VEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEIAH 190
           VEEIW+EL+KAKY EWE  S  RSWEL +LKE CEAAL Q+  LD S  E   DEA  AH
Sbjct: 126 VEEIWEELSKAKYMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAH 185

Query: 191 RKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKV 250
            ++LK+L  VFKK  E D P+EVPDYLCC ITL+I RDPVI+PSGVTYERA IL+H +KV
Sbjct: 186 TERLKALERVFKKAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKV 245

Query: 251 GNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAY 288
           G FDP+TRE +D + LVPNLAIKEAV A+L+KH WAY
Sbjct: 246 GKFDPITREKIDPANLVPNLAIKEAVAAYLEKHVWAY 274

[Close]

BLAST of Carg08887 vs. ExPASy Swiss-Prot
Match: Q9UNE7 (E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 4.4e-41
Identity = 98/284 (34.51%), Postives = 154/284 (54.23%), Query Frame = 0


Query: 15  AELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHRKRKYFFLMVYSDWK 74
           A+ L++ GN  F   +   A   Y  AIT  P VAVY TNRALC+ K +          +
Sbjct: 26  AQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQ--------QHE 85

Query: 75  KVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKSYMVEEI 134
           +   DCRRA+++D  SVK H+ LG   ++   Y E I +L++A  L +    +    ++I
Sbjct: 86  QALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAK--EQRLNFGDDI 145

Query: 135 WQELAKAKYREWEKASTKRSWELQTLKEACE--AALEQKYFLDHSEL--EGFVDEA---- 194
              L  AK + W     +R  +   L        A E++  L+  +   EG  D++    
Sbjct: 146 PSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRA 205

Query: 195 -----EIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERA 254
                E  H K +  +  +F +V E     ++PDYLC KI+ +++R+P ITPSG+TY+R 
Sbjct: 206 QQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRK 265

Query: 255 VILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGW 286
            I +H ++VG+FDP+TR  L + QL+PNLA+KE ++AF+ ++GW
Sbjct: 266 DIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 299

[Close]

BLAST of Carg08887 vs. ExPASy Swiss-Prot
Match: Q9WUD1 (E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.4e-41
Identity = 100/298 (33.56%), Postives = 157/298 (52.68%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           +G G       +  A+ L++ GN  F   +   A   Y  AIT  P VAVY TNRALC+ 
Sbjct: 13  LGTGGGGSPDKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYL 72

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           K +          ++   DCRRA+++D  SVK H+ LG   ++   Y E I +L++A  L
Sbjct: 73  KMQ--------QPEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSL 132

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACE--AALEQKYFLDHSE 180
            +    +    ++I   L  AK + W     +R  +   L        A E++  L+  +
Sbjct: 133 AK--EQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLTRLIAAERERELEECQ 192

Query: 181 L--EGFVDEA---------EIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILR 240
              EG  D+          E  H K +  +  +F +V E     ++PDYLC KI+ +++R
Sbjct: 193 RNHEGHEDDGHIRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMR 252

Query: 241 DPVITPSGVTYERAVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGW 286
           +P ITPSG+TY+R  I +H ++VG+FDP+TR  L + QL+PNLA+KE ++AF+ ++GW
Sbjct: 253 EPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 300

[Close]

BLAST of Carg08887 vs. ExPASy Swiss-Prot
Match: Q5ZHY5 (E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.7e-40
Identity = 98/281 (34.88%), Postives = 152/281 (54.09%), Query Frame = 0


Query: 19  RKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHRKRKYFFLMVYSDWKKVEE 78
           ++ GN  F   +   A  AY  AI   P VAVY TNRALC+ K +           K   
Sbjct: 40  KEQGNRLFGGRKYPEAAAAYGRAINRNPLVAVYYTNRALCYLKMQ--------QHDKALA 99

Query: 79  DCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKSYMVEEIWQEL 138
           DC+RA+++D  SVK H+ LG   ++   Y E I +L++A +L +    +    ++I   L
Sbjct: 100 DCKRALELDGQSVKAHFFLGQCQMEMENYDEAIANLQRAYNLAK--EQRLNFGDDIPSAL 159

Query: 139 AKAKYREWEKASTKR---SWELQTLKEACEAALEQKYFLD--HSELEGFVDEA------- 198
             AK + W     KR     EL +       A +++   +   ++ E  VDE+       
Sbjct: 160 RIAKKKRWNSIEEKRINQENELHSYLTRLIMAEKERELAECRKAQQEENVDESRGRVQLA 219

Query: 199 --EIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVIL 258
             E  H K L  +  +F +V E     ++PDYLC KI+ +++R+P ITPSG+TY+R  I 
Sbjct: 220 GIEAKHDKYLADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIE 279

Query: 259 DHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGW 286
           +H ++VG+FDP+TR  L + QL+PNLA+KE ++AF+ ++GW
Sbjct: 280 EHLQRVGHFDPVTRSPLTQDQLIPNLAMKEVIDAFISENGW 310

[Close]

BLAST of Carg08887 vs. ExPASy Swiss-Prot
Match: Q8VD33 (Small glutamine-rich tetratricopeptide repeat-containing protein beta OS=Mus musculus OX=10090 GN=Sgtb PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.3e-12
Identity = 43/107 (40.19%), Postives = 61/107 (57.01%), Query Frame = 0


Query: 14  QAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHRKRKYFFLMVYSDW 73
           +A+ L+ +GN++ ++    AA++ YT+AI L PN AVY  NRA    K     L  Y+D 
Sbjct: 84  KADQLKDEGNNHMKEENYAAAVDCYTQAIELDPNNAVYYCNRAAAQSK-----LSHYTDA 143

Query: 74  KKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 121
            K   DC +AI IDS   K +  +GLAL   N + E +   +KALDL
Sbjct: 144 IK---DCEKAIAIDSKYSKAYGRMGLALTAMNKFEEAVTSYQKALDL 182

[Close]

BLAST of Carg08887 vs. ExPASy TrEMBL
Match: A0A6J1K6X7 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC111491225 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 7.7e-158
Identity = 280/290 (96.55%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE
Sbjct: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEAEIAHRKQLKSLRSVF+KVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEAEIAHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD
Sbjct: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of Carg08887 vs. ExPASy TrEMBL
Match: A0A6J1EYU4 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437457 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 7.7e-158
Identity = 280/290 (96.55%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE
Sbjct: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEAEIAHRKQLKSLRSVF+KVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEAEIAHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD
Sbjct: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of Carg08887 vs. ExPASy TrEMBL
Match: A0A6J1DYV8 (E3 ubiquitin-protein ligase CHIP OS=Momordica charantia OX=3673 GN=LOC111025874 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.5e-145
Identity = 257/290 (88.62%), Postives = 272/290 (93.79%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPG VALSGAAKQAELLRKDGNHYFQKGR+GAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPG-VALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQ+D+NSVK HYMLGL+LIQN  YP GIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGANPKSY+VEEIWQELAKAKYREWE+A TKRSWELQTLKEACE ALEQKYFLDHSELE
Sbjct: 121 GRGANPKSYIVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GF+DEA+IAHRKQLK++RSVF+KV +ADAPSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFIDEADIAHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDP+TRELL ESQLVPNLAIKEAV+AFLDKHGWAYNTD
Sbjct: 241 AVILDHFRKVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 281

[Close]

BLAST of Carg08887 vs. ExPASy TrEMBL
Match: A0A0A0LBQ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634310 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.6e-142
Identity = 253/290 (87.24%), Postives = 269/290 (92.76%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPG V LSG AKQAE+LRKDGNHYFQKGRIGAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPG-VPLSGPAKQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DW KVEEDCRRAIQ+DS+SVK HYMLGLAL+QN  YPEGIKHLEKALDL
Sbjct: 61  KR--------NDWNKVEEDCRRAIQLDSSSVKAHYMLGLALLQNKEYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRG NPKSY+VEEIWQELAKAKYREWE+AST+RSWELQTLKEACEAALEQKYFLD SELE
Sbjct: 121 GRGENPKSYIVEEIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVDEA+IAHRKQLKSLRSVF+KV EAD+PSEVPDYLCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDEADIAHRKQLKSLRSVFEKVTEADSPSEVPDYLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHF KVGNFDP+TRELL+ESQL+PNLAIKEAV++FLDKHGWAYN D
Sbjct: 241 AVILDHFNKVGNFDPITRELLNESQLIPNLAIKEAVQSFLDKHGWAYNMD 281

[Close]

BLAST of Carg08887 vs. ExPASy TrEMBL
Match: A0A6J1HID6 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111463871 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.9e-140
Identity = 252/290 (86.90%), Postives = 266/290 (91.72%), Query Frame = 0


Query: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60
           MGPG V LSGAA+QAE+LRKDGNHYFQKGRIGAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPG-VFLSGAARQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHR 60

Query: 61  KRKYFFLMVYSDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDL 120
           KR        +DWKKVEEDCRRAIQ+D+NSVK HYM+GLALIQN  YPEGIKHLEKALDL
Sbjct: 61  KR--------NDWKKVEEDCRRAIQLDNNSVKAHYMVGLALIQNKDYPEGIKHLEKALDL 120

Query: 121 GRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELE 180
           GRGAN KSYMVEEIWQELA AKYREWE+ASTKRSWELQTLKEACEAALEQKYFLD SE E
Sbjct: 121 GRGANQKSYMVEEIWQELANAKYREWEQASTKRSWELQTLKEACEAALEQKYFLDQSEPE 180

Query: 181 GFVDEAEIAHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYER 240
           GFVD+AE AHR+QL SLRSVF+    ADAPSEVPD+LCCKITLDILRDPVITPSGVTYER
Sbjct: 181 GFVDQAESAHREQLNSLRSVFENYAVADAPSEVPDHLCCKITLDILRDPVITPSGVTYER 240

Query: 241 AVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 291
           AVILDHFRKVGNFDP+TRELLDESQLVPNLAIKEAV+AFLDKHGWAYNT+
Sbjct: 241 AVILDHFRKVGNFDPITRELLDESQLVPNLAIKEAVQAFLDKHGWAYNTN 281

[Close]

BLAST of Carg08887 vs. TAIR 10
Match: AT3G07370.1 (carboxyl terminus of HSC70-interacting protein )

HSP 1 Score: 362.5 bits (929), Expect = 3.3e-100
Identity = 178/277 (64.26%), Postives = 209/277 (75.45%), Query Frame = 0


Query: 11  AAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHRKRKYFFLMVY 70
           A+  AE L++DGN+ F+K R GAAI+AYTEAI L PNV  Y TNRALCH KRK       
Sbjct: 6   ASAMAERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRK------- 65

Query: 71  SDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKSYM 130
            DW KVEEDCR+AIQ+  NSVK HYMLGLAL+Q   +  G+K L++ALDLGR +NP  YM
Sbjct: 66  -DWTKVEEDCRKAIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYM 125

Query: 131 VEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEIAH 190
           VEEIW+EL+KAKY EWE  S  RSWEL +LKE CEAAL Q+  LD S  E   DEA  AH
Sbjct: 126 VEEIWEELSKAKYMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAH 185

Query: 191 RKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKV 250
            ++LK+L  VFKK  E D P+EVPDYLCC ITL+I RDPVI+PSGVTYERA IL+H +KV
Sbjct: 186 TERLKALERVFKKAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKV 245

Query: 251 GNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAY 288
           G FDP+TRE +D + LVPNLAIKEAV A+L+KH WAY
Sbjct: 246 GKFDPITREKIDPANLVPNLAIKEAVAAYLEKHVWAY 274

[Close]

BLAST of Carg08887 vs. TAIR 10
Match: AT3G61390.2 (RING/U-box superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 3.7e-11
Identity = 52/180 (28.89%), Postives = 83/180 (46.11%), Query Frame = 0


Query: 115 EKALDLGRGANPKSYMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFL 174
           E+A      A  K+   E ++ E  K + +E EKA  KR+ EL+ ++   E  + + Y +
Sbjct: 243 EEAEKTAVDALKKAKQWENVYFEELKQR-KETEKALRKRNDELEKMRSESETQITESYTV 302

Query: 175 -------DHSELEGFVDEAEIAHRKQLKSLRSVFKKVGEADAPS-------EVPDYLCCK 234
                  ++  +E F    E     ++K LR V K  G+ +          E P Y  C 
Sbjct: 303 IRKLQEKNNLSMETFRGIREEQEELKIK-LREVSKLKGKREEEEASTSNHREPPQYFICP 362

Query: 235 ITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVEAFL 281
           IT DI+ DP +   G TYE   I   F +     P+  + L  + LVPNLA++ A++ +L
Sbjct: 363 ITHDIMEDPHVAADGFTYEGEAISRWFERGHETSPMINKRLPHTSLVPNLALRSAIQEWL 420

[Close]

BLAST of Carg08887 vs. TAIR 10
Match: AT2G45920.1 (U-box domain-containing protein )

HSP 1 Score: 62.4 bits (150), Expect = 7.0e-10
Identity = 50/183 (27.32%), Postives = 82/183 (44.81%), Query Frame = 0


Query: 109 EGIKH----LEKALDLGRGANPKSYMVEEIWQ----ELAKAKYREWEKASTKRSWELQTL 168
           E  KH     EK   + RG   +S  +EE+ Q    E+   K RE  +     S    T 
Sbjct: 210 EAFKHQEVVKEKNEAIKRGKEWESAYLEELKQRKETEMELKKVREKLEKMRYISENRITE 269

Query: 169 KEACEAALEQKYFLDHSELEGFVDEAEIAHRKQ---LKSLRSVFKKVGEADAPSEVPDYL 228
                  L+ KY L    L    +E ++  + +   +  +  + K+V  +D   E P Y 
Sbjct: 270 SYMLVQKLQDKYNLATKVLRKAKEERDLLIKGRDIAIIEVEELRKEVSRSDEHREAPQYF 329

Query: 229 CCKITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPLTRELLDESQLVPNLAIKEAVE 281
            C I+L++++DP +   G TYE   I    +      P+T   L  ++LVPNLA++ A++
Sbjct: 330 ICPISLEVMKDPQLAADGFTYEAEAISTWLQGGHETSPMTNTKLHHTKLVPNLALRSAIQ 389

[Close]

BLAST of Carg08887 vs. TAIR 10
Match: AT3G18710.1 (plant U-box 29 )

HSP 1 Score: 58.9 bits (141), Expect = 7.7e-09
Identity = 30/85 (35.29%), Postives = 43/85 (50.59%), Query Frame = 0


Query: 206 EADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPLTRELLDESQ 265
           E +    VP +  C I+LD++R PV   +GVTY+RA I        N  P T +LL    
Sbjct: 5   ETETYITVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKD 64

Query: 266 LVPNLAIKEAVEAFLDKHGWAYNTD 291
            VPNL ++  +  + D  G  +N D
Sbjct: 65  FVPNLTLQRLINIWSDSIGRRHNGD 89

[Close]

BLAST of Carg08887 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 58.5 bits (140), Expect = 1.0e-08
Identity = 28/79 (35.44%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 200 VFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPLTRE 259
           VF ++ + +   E+P+Y  C I+L+I++DPV T SG+TY+R  I+    KV +  P+T++
Sbjct: 11  VFPQMDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVPSC-PVTKQ 70

Query: 260 LLD-ESQLVPNLAIKEAVE 278
            L  +S L PN  ++  ++
Sbjct: 71  PLPLDSDLTPNHMLRRLIQ 88

The following BLAST results are available for this feature:

BLAST of Carg08887 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036240.1	1.6e-165	100.00	E3 ubiquitin-protein ligase CHIP [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6606301.1	5.5e-158	96.90	E3 ubiquitin-protein ligase CHIP, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022931210.1	1.6e-157	96.55	E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ub...	[more]
XP_023533768.1	1.8e-156	95.86	E3 ubiquitin-protein ligase CHIP-like [Cucurbita pepo subsp. pepo]	[more]
XP_038886937.1	4.8e-146	90.66	E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida]	[more]

BLAST of Carg08887 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRS9	4.6e-99	64.26	E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV...	[more]
Q9UNE7	4.4e-41	34.51	E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2	[more]
Q9WUD1	4.4e-41	33.56	E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1	[more]
Q5ZHY5	3.7e-40	34.88	E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1	[more]
Q8VD33	3.3e-12	40.19	Small glutamine-rich tetratricopeptide repeat-containing protein beta OS=Mus mus...	[more]

BLAST of Carg08887 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K6X7	7.7e-158	96.55	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC11149122...	[more]
A0A6J1EYU4	7.7e-158	96.55	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437...	[more]
A0A6J1DYV8	1.5e-145	88.62	E3 ubiquitin-protein ligase CHIP OS=Momordica charantia OX=3673 GN=LOC111025874 ...	[more]
A0A0A0LBQ5	1.6e-142	87.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634310 PE=4 SV=1	[more]
A0A6J1HID6	1.9e-140	86.90	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111463...	[more]

BLAST of Carg08887 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07370.1	3.3e-100	64.26	carboxyl terminus of HSC70-interacting protein	[more]
AT3G61390.2	3.7e-11	28.89	RING/U-box superfamily protein	[more]
AT2G45920.1	7.0e-10	27.32	U-box domain-containing protein	[more]
AT3G18710.1	7.7e-09	35.29	plant U-box 29	[more]
AT3G11840.1	1.0e-08	35.44	plant U-box 24	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019734	Tetratricopeptide repeat	SMART	SM00028	tpr_5	coord: 15..48 e-value: 0.0026 score: 27.0 coord: 49..90 e-value: 83.0 score: 6.9 coord: 91..124 e-value: 8.4 score: 15.3
IPR019734	Tetratricopeptide repeat	PFAM	PF13181	TPR_8	coord: 25..47 e-value: 0.0077 score: 16.4
IPR019734	Tetratricopeptide repeat	PROSITE	PS50005	TPR	coord: 15..48 score: 9.7354
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 216..279 e-value: 2.4E-22 score: 90.2
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 213..285 e-value: 5.7E-26 score: 90.5
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 212..286 score: 30.864414
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 211..289 e-value: 2.9E-25 score: 90.0
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 10..153 e-value: 4.6E-29 score: 102.8
IPR011990	Tetratricopeptide-like helical domain superfamily	SUPERFAMILY	48452	TPR-like	coord: 13..143
None	No IPR available	PANTHER	PTHR46803:SF3	OS08G0113300 PROTEIN	coord: 9..288
None	No IPR available	PANTHER	PTHR46803	E3 UBIQUITIN-PROTEIN LIGASE CHIP	coord: 9..288
None	No IPR available	CDD	cd16654	RING-Ubox_CHIP	coord: 213..279 e-value: 4.4791E-41 score: 134.68
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 207..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08887-RA	Carg08887-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071218	cellular response to misfolded protein
biological_process	GO:0045862	positive regulation of proteolysis
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0009651	response to salt stress
biological_process	GO:0009266	response to temperature stimulus
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity