Carg08880 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg08880 vs. NCBI nr
Match: KAG7036233.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL
Sbjct: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
           SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL
Sbjct: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK
Sbjct: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS
Sbjct: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540

Query: 541 VSADLPDLHFANRDRPDTLVY 562
           VSADLPDLHFANRDRPDTLVY
Sbjct: 541 VSADLPDLHFANRDRPDTLVY 561

BLAST of Carg08880 vs. NCBI nr
Match: KAG6606291.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 560/561 (99.82%), Postives = 560/561 (99.82%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL
Sbjct: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
           SSAETEPPIVGDSDRQKF TSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL
Sbjct: 61  SSAETEPPIVGDSDRQKFSTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK
Sbjct: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS
Sbjct: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540

Query: 541 VSADLPDLHFANRDRPDTLVY 562
           VSADLPDLHFANRDRPDTLVY
Sbjct: 541 VSADLPDLHFANRDRPDTLVY 561

BLAST of Carg08880 vs. NCBI nr
Match: XP_022931075.1 (probable polyamine transporter At1g31830 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 555/561 (98.93%), Postives = 556/561 (99.11%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDGAEKEAP SALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSD AKEEL
Sbjct: 1   MKRDGAEKEAPASALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDSAKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
           SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSS VNNAKKVSVLPL
Sbjct: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSLVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFG ILEFLAFIKLRIKHP ASRPYK
Sbjct: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGTILEFLAFIKLRIKHPTASRPYK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGTVGSILMCIPPTILICVVLALSTVKV+IVSLAAVAVGLLLHPGLGYVEKKRWLKFS
Sbjct: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVVIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540

Query: 541 VSADLPDLHFANRDRPDTLVY 562
           VSADLPDLHFANRDRPDTLVY
Sbjct: 541 VSADLPDLHFANRDRPDTLVY 561

BLAST of Carg08880 vs. NCBI nr
Match: XP_023532711.1 (probable polyamine transporter At1g31830 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 553/561 (98.57%), Postives = 555/561 (98.93%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDG EKEA VSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSD A EEL
Sbjct: 1   MKRDGVEKEAAVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDGATEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
           SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL
Sbjct: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWV+SALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV
Sbjct: 181 VWVTSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNL LNT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLCLNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK
Sbjct: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGT+GSILMCIPPTILICVVLA STVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS
Sbjct: 481 IPVGTIGSILMCIPPTILICVVLAFSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540

Query: 541 VSADLPDLHFANRDRPDTLVY 562
           VSADLPDLHFANRDRPDTLVY
Sbjct: 541 VSADLPDLHFANRDRPDTLVY 561

BLAST of Carg08880 vs. NCBI nr
Match: XP_022995527.1 (probable polyamine transporter At1g31830 [Cucurbita maxima])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.9e-301
Identity = 536/552 (97.10%), Postives = 543/552 (98.37%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDGAEKEAP+SALPVDPKPE+AATAAPPATELGEKEVQAQSAAAA APSHSD AKEEL
Sbjct: 1   MKRDGAEKEAPISALPVDPKPEKAATAAPPATELGEKEVQAQSAAAATAPSHSDGAKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
           SSAETE PIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL
Sbjct: 61  SSAETELPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEAL+TAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLP+VAA LALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGL+SIPKLRPSRWLVVNLKDVDWNLY NT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWITGAAA+SNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPLIGILFSASGVVLLSWLSFQEIIA ENFLYCFGMILEFLAFI LRIKHPAASRP+K
Sbjct: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGTVGSILMCIPPTILICVVLALS VKVMIVSLAAVAVGLLLHPGL YVEKKRWLKFS
Sbjct: 481 IPVGTVGSILMCIPPTILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFS 540

Query: 541 VSADLPDLHFAN 553
           VSADLPDLHFAN
Sbjct: 541 VSADLPDLHFAN 552

BLAST of Carg08880 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 5.0e-219
Identity = 370/491 (75.36%), Postives = 428/491 (87.17%), Query Frame = 0

Query: 75  RQKFGTSPARQVSVAMGELNRAEYVSVG--ESPSSP---VNNAKKVSVLPLVFLIFYEVS 134
           R+    +P+  + ++  E N   Y SVG  E PSSP    +  +KVS+LPLVFLIFYEVS
Sbjct: 4   RRIITVNPSASIEMSQYENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVS 63

Query: 135 GGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGP 194
           GGPFGVEDSV AAGPLLALLGF++FP IWSIPEALITAEMGTM+PENGGYVVWVSSALGP
Sbjct: 64  GGPFGVEDSVNAAGPLLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGP 123

Query: 195 FWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRG 254
           FWGFQQGWMKWLSGVIDNALYPVLFLDYLKS +PALG GLPRVA++L LT++LTY+NYRG
Sbjct: 124 FWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRG 183

Query: 255 LTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWD 314
           LTIVGWVAV++GVFSILPFAVMGL+SIP+L PSRWLV++L +V+WNLYLNTLFWNLNYWD
Sbjct: 184 LTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWD 243

Query: 315 SISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKI 374
           SISTLAGEVENPN TLPKALFY +ILV   Y FPLL+G GAIPL R+ WTDGYFSDVAK 
Sbjct: 244 SISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKA 303

Query: 375 IGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGIL 434
           +GGAWL WW+  AAA SNMGMF+AEMSSDSFQLLGMAERGMLPEFF KRSR+GTPL+GIL
Sbjct: 304 LGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGIL 363

Query: 435 FSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSI 494
           FSASGVVLLSWLSFQEI+AAEN LYC GMILEF+AF+++R+KHPAASRPYKIP+GT GSI
Sbjct: 364 FSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSI 423

Query: 495 LMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLH 554
           LMCIPPTILIC V+ALS++KV  VS+  + +G L+HP L ++++KRW+KFS+S+DLPDL 
Sbjct: 424 LMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQ 483

Query: 555 FANRDRPDTLV 561
              R+  +TL+
Sbjct: 484 QQTREYEETLI 494

BLAST of Carg08880 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 7.3e-202
Identity = 349/510 (68.43%), Postives = 421/510 (82.55%), Query Frame = 0

Query: 41  AQSAAAAAAPSHSDDAKEELSSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVS 100
           A + AAAAA     D  +E  + E+  P  G +   + GT                EY  
Sbjct: 23  ASTTAAAAADLGHADTGQEKPTVESAQPANGAAPMGECGT----------------EYRG 82

Query: 101 V--GESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIW 160
           +  G++     ++A+ VS++PL+FLIFYEVSGGPFG+EDSVGAAGPLLA++GFLV P+IW
Sbjct: 83  LPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIW 142

Query: 161 SIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYL 220
           SIPEALITAE+G MFPENGGYVVWV+SALGP+WGFQQGWMKWLSGVIDNALYPVLFLDYL
Sbjct: 143 SIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYL 202

Query: 221 KSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPK 280
           KS +PALGGG PR  AV+ LT VLT +NYRGLT+VGWVA+ LGVFS+LPF VMGL+++PK
Sbjct: 203 KSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPK 262

Query: 281 LRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVL 340
           LRP+RWLV++L +VDWNLYLNTLFWNLNYWDSISTLAGEV+NP KTLPKALFYA+I VV+
Sbjct: 263 LRPARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVV 322

Query: 341 GYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSD 400
            Y +PLL+GTGA+PL+R  WTDGYF+D+AK++GGAWL WW+  AAA+SNMGMFVAEMSSD
Sbjct: 323 AYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSD 382

Query: 401 SFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGM 460
           S+QLLGMAERGMLP FF  RSR+GTPL GILFSASGV+LLS +SFQEI+AAENFLYCFGM
Sbjct: 383 SYQLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGM 442

Query: 461 ILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAV 520
           +LEF+AFI  R++ P A+RPY++P+GT G + M +PPT LI VVLALST+KV +VSL AV
Sbjct: 443 LLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAV 502

Query: 521 AVGLLLHPGLGYVEKKRWLKFSVSADLPDL 549
           A+GL+L P L +VEKKRWL+FSV+ DLP++
Sbjct: 503 AMGLVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of Carg08880 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 7.3e-202
Identity = 349/510 (68.43%), Postives = 421/510 (82.55%), Query Frame = 0

Query: 41  AQSAAAAAAPSHSDDAKEELSSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVS 100
           A + AAAAA     D  +E  + E+  P  G +   + GT                EY  
Sbjct: 23  ASTTAAAAADLGHADTGQEKPTVESAQPANGAAPMGECGT----------------EYRG 82

Query: 101 V--GESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIW 160
           +  G++     ++A+ VS++PL+FLIFYEVSGGPFG+EDSVGAAGPLLA++GFLV P+IW
Sbjct: 83  LPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIW 142

Query: 161 SIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYL 220
           SIPEALITAE+G MFPENGGYVVWV+SALGP+WGFQQGWMKWLSGVIDNALYPVLFLDYL
Sbjct: 143 SIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYL 202

Query: 221 KSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPK 280
           KS +PALGGG PR  AV+ LT VLT +NYRGLT+VGWVA+ LGVFS+LPF VMGL+++PK
Sbjct: 203 KSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPK 262

Query: 281 LRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVL 340
           LRP+RWLV++L +VDWNLYLNTLFWNLNYWDSISTLAGEV+NP KTLPKALFYA+I VV+
Sbjct: 263 LRPARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVV 322

Query: 341 GYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSD 400
            Y +PLL+GTGA+PL+R  WTDGYF+D+AK++GGAWL WW+  AAA+SNMGMFVAEMSSD
Sbjct: 323 AYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSD 382

Query: 401 SFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGM 460
           S+QLLGMAERGMLP FF  RSR+GTPL GILFSASGV+LLS +SFQEI+AAENFLYCFGM
Sbjct: 383 SYQLLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGM 442

Query: 461 ILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAV 520
           +LEF+AFI  R++ P A+RPY++P+GT G + M +PPT LI VVLALST+KV +VSL AV
Sbjct: 443 LLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAV 502

Query: 521 AVGLLLHPGLGYVEKKRWLKFSVSADLPDL 549
           A+GL+L P L +VEKKRWL+FSV+ DLP++
Sbjct: 503 AMGLVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of Carg08880 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 4.7e-201
Identity = 336/447 (75.17%), Postives = 396/447 (88.59%), Query Frame = 0

Query: 104 SPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEA 163
           SP+  VN  KK++VLPLVFLIFYEVSGGPFG+EDSV AAGPLLA++GF+VFP IWSIPEA
Sbjct: 42  SPARTVNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEA 101

Query: 164 LITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIP 223
           LITAEMGTMFPENGGYVVWV+ A+GP+WGFQQGW+KWLSGVIDNALYP+LFLDYLKS IP
Sbjct: 102 LITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIP 161

Query: 224 ALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSR 283
            LG G+PRVAA+L LTV LTY+NYRGL+IVG  AV+LGVFSILPF VM  +SIPKL+PSR
Sbjct: 162 ILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSR 221

Query: 284 WLVVN--LKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYF 343
           WLVV+  +K V+W+LYLNTLFWNLNYWDS+STL GEVENP+KTLP+ALFYAL+LVV  Y 
Sbjct: 222 WLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYI 281

Query: 344 FPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQ 403
           FP+L+GTGAI L++ LWTDGYF+D+ K+IGG WL WWI  AAA SNMGMF+AEMSSDSFQ
Sbjct: 282 FPVLTGTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQ 341

Query: 404 LLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILE 463
           LLGMAERGMLPE F KRSR+ TP +GILFSASGV++LSWLSFQEI+AAEN LYCFGM+LE
Sbjct: 342 LLGMAERGMLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLE 401

Query: 464 FLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVG 523
           F+ F++LR+K+PAASRP+KIPVG +GS+LMCIPPT+LI V++A + +KV +VSLAA+ +G
Sbjct: 402 FITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIG 461

Query: 524 LLLHPGLGYVEKKRWLKFSVSADLPDL 549
           L+L P L  VEKK WLKFS S+ LP+L
Sbjct: 462 LVLQPCLKQVEKKGWLKFSTSSHLPNL 488

BLAST of Carg08880 vs. ExPASy Swiss-Prot
Match: Q9C6S4 (Probable polyamine transporter At1g31820 OS=Arabidopsis thaliana OX=3702 GN=At1g31820 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 3.3e-194
Identity = 337/472 (71.40%), Postives = 392/472 (83.05%), Query Frame = 0

Query: 89  AMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLAL 148
           A G L   +   VG S     N+ +KVS+LPLVFLIFYEVSGGPFG E SV AAGPLLAL
Sbjct: 10  AYGNLYDDDDGDVGGSSKEGNNSIQKVSMLPLVFLIFYEVSGGPFGAEGSVNAAGPLLAL 69

Query: 149 LGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 208
           LGF++FP IW IPEALITAEM TMFP NGG+VVWVSSALG FWGFQ GWMKWL GVIDNA
Sbjct: 70  LGFVIFPFIWCIPEALITAEMSTMFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNA 129

Query: 209 LYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSILPF 268
           LYPVLFLDYLKS +PAL  GLPRVA++L LT++LTY+NYRGLTIVGW AV +GVFS+LPF
Sbjct: 130 LYPVLFLDYLKSAVPALATGLPRVASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPF 189

Query: 269 AVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLPKA 328
           AVM LVSIP+L PSRWLV++L +V+WNLYLNTL WNLNYWDS+STLAGEV NP KTLPKA
Sbjct: 190 AVMSLVSIPQLEPSRWLVMDLGNVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKA 249

Query: 329 LFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAMSNM 388
           L Y +I V L  F PLLSGTGAIPL+R+LWTDGY ++VAK IGG WL  W+  AAA SNM
Sbjct: 250 LCYGVIFVALSNFLPLLSGTGAIPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNM 309

Query: 389 GMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIA 448
           GMF+AEMSSDSFQLLGMAE G+LPE F +RSR+GTPL+GILFSASGV+LLS LSFQEIIA
Sbjct: 310 GMFLAEMSSDSFQLLGMAELGILPEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIA 369

Query: 449 AENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALSTV 508
           AEN LYC GMILEF+AF++LR KHPAASRPYKIPVGTVGSIL+C+PP +LIC+V+ LST+
Sbjct: 370 AENLLYCGGMILEFIAFVRLRKKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTI 429

Query: 509 KVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDRPDTLV 561
           KV +VS   V +G L+ P L +++ K+W+KFSV +DL +    N D  ++L+
Sbjct: 430 KVALVSFVMVVIGFLMKPCLNHMDGKKWVKFSVCSDLAEFQKENLDCEESLL 481

BLAST of Carg08880 vs. ExPASy TrEMBL
Match: A0A6J1EYH3 (probable polyamine transporter At1g31830 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437371 PE=3 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 555/561 (98.93%), Postives = 556/561 (99.11%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDGAEKEAP SALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSD AKEEL
Sbjct: 1   MKRDGAEKEAPASALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDSAKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
           SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSS VNNAKKVSVLPL
Sbjct: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSLVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFG ILEFLAFIKLRIKHP ASRPYK
Sbjct: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGTILEFLAFIKLRIKHPTASRPYK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGTVGSILMCIPPTILICVVLALSTVKV+IVSLAAVAVGLLLHPGLGYVEKKRWLKFS
Sbjct: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVVIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540

Query: 541 VSADLPDLHFANRDRPDTLVY 562
           VSADLPDLHFANRDRPDTLVY
Sbjct: 541 VSADLPDLHFANRDRPDTLVY 561

BLAST of Carg08880 vs. ExPASy TrEMBL
Match: A0A6J1JZ60 (probable polyamine transporter At1g31830 OS=Cucurbita maxima OX=3661 GN=LOC111491032 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.9e-301
Identity = 536/552 (97.10%), Postives = 543/552 (98.37%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDGAEKEAP+SALPVDPKPE+AATAAPPATELGEKEVQAQSAAAA APSHSD AKEEL
Sbjct: 1   MKRDGAEKEAPISALPVDPKPEKAATAAPPATELGEKEVQAQSAAAATAPSHSDGAKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
           SSAETE PIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL
Sbjct: 61  SSAETELPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEAL+TAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALVTAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLP+VAA LALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPKVAAALALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGL+SIPKLRPSRWLVVNLKDVDWNLY NT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLISIPKLRPSRWLVVNLKDVDWNLYWNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWITGAAA+SNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWITGAAAISNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPLIGILFSASGVVLLSWLSFQEIIA ENFLYCFGMILEFLAFI LRIKHPAASRP+K
Sbjct: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAVENFLYCFGMILEFLAFIMLRIKHPAASRPFK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGTVGSILMCIPPTILICVVLALS VKVMIVSLAAVAVGLLLHPGL YVEKKRWLKFS
Sbjct: 481 IPVGTVGSILMCIPPTILICVVLALSVVKVMIVSLAAVAVGLLLHPGLRYVEKKRWLKFS 540

Query: 541 VSADLPDLHFAN 553
           VSADLPDLHFAN
Sbjct: 541 VSADLPDLHFAN 552

BLAST of Carg08880 vs. ExPASy TrEMBL
Match: A0A6J1HQ90 (probable polyamine transporter At1g31830 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466824 PE=3 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.1e-276
Identity = 497/561 (88.59%), Postives = 516/561 (91.98%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           M+RDG   EAP+S   +D KPEQ A+AA    ELGEKE Q +S   A +P H D  KEEL
Sbjct: 1   MRRDG--DEAPISTSQIDRKPEQTASAA----ELGEKEFQPKS-TGAGSPLHCDSTKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
             AETEPPIVGDS+ QK  +SPARQ SVAMGE+NRAEYVSVGE PS  VNNAKKVSVLPL
Sbjct: 61  RRAETEPPIVGDSNHQKLRSSPARQTSVAMGEINRAEYVSVGELPSPSVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALG GLPRVAAVLALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGEGLPRVAAVLALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           VLTYMNYRGLTIVGWVAVILG FSILPF VMGLVSIPKL+PSRW VVNL+DVDWNLYLNT
Sbjct: 241 VLTYMNYRGLTIVGWVAVILGAFSILPFVVMGLVSIPKLQPSRWFVVNLRDVDWNLYLNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVL YF PLLSGTGAI LNR+LWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLSYFLPLLSGTGAIALNRELWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWI GAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFF +RSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWIQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSQRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPL GILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAF+KLRIKHPAASRPYK
Sbjct: 421 HGTPLTGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFVKLRIKHPAASRPYK 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGT+GSILMCIPPTILIC+VLALSTVKVMIVSLAAVA+GLLL PGL YVEKKRWLKFS
Sbjct: 481 IPVGTIGSILMCIPPTILICIVLALSTVKVMIVSLAAVAIGLLLQPGLKYVEKKRWLKFS 540

Query: 541 VSADLPDLHFANRDRPDTLVY 562
           VS DLPDLHFANRDR +T+VY
Sbjct: 541 VSGDLPDLHFANRDRTETMVY 554

BLAST of Carg08880 vs. ExPASy TrEMBL
Match: A0A6J1HJB1 (probable polyamine transporter At1g31830 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464079 PE=3 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.1e-276
Identity = 499/561 (88.95%), Postives = 515/561 (91.80%), Query Frame = 0

Query: 1   MKRDGAEKEAPVSALPVDPKPEQAATAAPPATELGEKEVQAQSAAAAAAPSHSDDAKEEL 60
           MKRDG   EAP+SA  +D KPEQ  +AA    ELGEKE Q  S  +A +P H D  KEEL
Sbjct: 1   MKRDG--DEAPISASQIDRKPEQTTSAA----ELGEKEFQPNS-TSAGSPLHCDGTKEEL 60

Query: 61  SSAETEPPIVGDSDRQKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPL 120
             AETEPPIVGDS  QK  +SPARQ SVAMGE+NRAEYVSVGE PS  VNNAKKVSVLPL
Sbjct: 61  RRAETEPPIVGDSSHQKLRSSPARQTSVAMGEINRAEYVSVGELPSPLVNNAKKVSVLPL 120

Query: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180
           VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV
Sbjct: 121 VFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYV 180

Query: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTV 240
           VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIP LG GLPRVAAVLALTV
Sbjct: 181 VWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPVLGEGLPRVAAVLALTV 240

Query: 241 VLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNT 300
           +LTYMNYRGLTIVGWVAVILG FSILPFAVMGLVSIPKL+PSRW VVNLKDVDWNLYLNT
Sbjct: 241 ILTYMNYRGLTIVGWVAVILGAFSILPFAVMGLVSIPKLQPSRWFVVNLKDVDWNLYLNT 300

Query: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTD 360
           LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVL YF PLLSGTGAI LNR+LWTD
Sbjct: 301 LFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLSYFLPLLSGTGAIALNRELWTD 360

Query: 361 GYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSR 420
           GYFSDVAKIIGGAWLSWWI GAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFF +RSR
Sbjct: 361 GYFSDVAKIIGGAWLSWWIQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSQRSR 420

Query: 421 HGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYK 480
           HGTPL GILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAF+KLRIKHPAASRPY+
Sbjct: 421 HGTPLTGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFVKLRIKHPAASRPYR 480

Query: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFS 540
           IPVGTVGSILMCIPPTILICVVLALSTVKVM+VSLAAVA+GLLL PGL YVEKKRWLKFS
Sbjct: 481 IPVGTVGSILMCIPPTILICVVLALSTVKVMLVSLAAVAIGLLLQPGLKYVEKKRWLKFS 540

Query: 541 VSADLPDLHFANRDRPDTLVY 562
           VS DLPDLHFANRDR +TLVY
Sbjct: 541 VSGDLPDLHFANRDRTETLVY 554

BLAST of Carg08880 vs. ExPASy TrEMBL
Match: A0A6J1ESP8 (probable polyamine transporter At1g31830 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437371 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 9.7e-274
Identity = 481/485 (99.18%), Postives = 482/485 (99.38%), Query Frame = 0

Query: 77  KFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVE 136
           KFGTSPARQVSVAMGELNRAEYVSVGESPSS VNNAKKVSVLPLVFLIFYEVSGGPFGVE
Sbjct: 2   KFGTSPARQVSVAMGELNRAEYVSVGESPSSLVNNAKKVSVLPLVFLIFYEVSGGPFGVE 61

Query: 137 DSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQG 196
           DSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQG
Sbjct: 62  DSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQG 121

Query: 197 WMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWV 256
           WMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWV
Sbjct: 122 WMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWV 181

Query: 257 AVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAG 316
           AVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAG
Sbjct: 182 AVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAG 241

Query: 317 EVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLS 376
           EVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLS
Sbjct: 242 EVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLS 301

Query: 377 WWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVV 436
           WWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVV
Sbjct: 302 WWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVV 361

Query: 437 LLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPT 496
           LLSWLSFQEIIAAENFLYCFG ILEFLAFIKLRIKHP ASRPYKIPVGTVGSILMCIPPT
Sbjct: 362 LLSWLSFQEIIAAENFLYCFGTILEFLAFIKLRIKHPTASRPYKIPVGTVGSILMCIPPT 421

Query: 497 ILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDRP 556
           ILICVVLALSTVKV+IVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDRP
Sbjct: 422 ILICVVLALSTVKVVIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDRP 481

Query: 557 DTLVY 562
           DTLVY
Sbjct: 482 DTLVY 486

BLAST of Carg08880 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 761.9 bits (1966), Expect = 3.6e-220
Identity = 370/491 (75.36%), Postives = 428/491 (87.17%), Query Frame = 0

Query: 75  RQKFGTSPARQVSVAMGELNRAEYVSVG--ESPSSP---VNNAKKVSVLPLVFLIFYEVS 134
           R+    +P+  + ++  E N   Y SVG  E PSSP    +  +KVS+LPLVFLIFYEVS
Sbjct: 4   RRIITVNPSASIEMSQYENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVS 63

Query: 135 GGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGP 194
           GGPFGVEDSV AAGPLLALLGF++FP IWSIPEALITAEMGTM+PENGGYVVWVSSALGP
Sbjct: 64  GGPFGVEDSVNAAGPLLALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGP 123

Query: 195 FWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRG 254
           FWGFQQGWMKWLSGVIDNALYPVLFLDYLKS +PALG GLPRVA++L LT++LTY+NYRG
Sbjct: 124 FWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRG 183

Query: 255 LTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWD 314
           LTIVGWVAV++GVFSILPFAVMGL+SIP+L PSRWLV++L +V+WNLYLNTLFWNLNYWD
Sbjct: 184 LTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWD 243

Query: 315 SISTLAGEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKI 374
           SISTLAGEVENPN TLPKALFY +ILV   Y FPLL+G GAIPL R+ WTDGYFSDVAK 
Sbjct: 244 SISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKA 303

Query: 375 IGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGIL 434
           +GGAWL WW+  AAA SNMGMF+AEMSSDSFQLLGMAERGMLPEFF KRSR+GTPL+GIL
Sbjct: 304 LGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGIL 363

Query: 435 FSASGVVLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSI 494
           FSASGVVLLSWLSFQEI+AAEN LYC GMILEF+AF+++R+KHPAASRPYKIP+GT GSI
Sbjct: 364 FSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSI 423

Query: 495 LMCIPPTILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLH 554
           LMCIPPTILIC V+ALS++KV  VS+  + +G L+HP L ++++KRW+KFS+S+DLPDL 
Sbjct: 424 LMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQ 483

Query: 555 FANRDRPDTLV 561
              R+  +TL+
Sbjct: 484 QQTREYEETLI 494

BLAST of Carg08880 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 758.1 bits (1956), Expect = 5.1e-219
Identity = 368/474 (77.64%), Postives = 420/474 (88.61%), Query Frame = 0

Query: 92  ELNRAEYVSVG--ESPSSP---VNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLL 151
           E N   Y SVG  E PSSP    +  +KVS+LPLVFLIFYEVSGGPFGVEDSV AAGPLL
Sbjct: 5   ENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLL 64

Query: 152 ALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 211
           ALLGF++FP IWSIPEALITAEMGTM+PENGGYVVWVSSALGPFWGFQQGWMKWLSGVID
Sbjct: 65  ALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 124

Query: 212 NALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSIL 271
           NALYPVLFLDYLKS +PALG GLPRVA++L LT++LTY+NYRGLTIVGWVAV++GVFSIL
Sbjct: 125 NALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSIL 184

Query: 272 PFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLP 331
           PFAVMGL+SIP+L PSRWLV++L +V+WNLYLNTLFWNLNYWDSISTLAGEVENPN TLP
Sbjct: 185 PFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLP 244

Query: 332 KALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAMS 391
           KALFY +ILV   Y FPLL+G GAIPL R+ WTDGYFSDVAK +GGAWL WW+  AAA S
Sbjct: 245 KALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATS 304

Query: 392 NMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEI 451
           NMGMF+AEMSSDSFQLLGMAERGMLPEFF KRSR+GTPL+GILFSASGVVLLSWLSFQEI
Sbjct: 305 NMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEI 364

Query: 452 IAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALS 511
           +AAEN LYC GMILEF+AF+++R+KHPAASRPYKIP+GT GSILMCIPPTILIC V+ALS
Sbjct: 365 VAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALS 424

Query: 512 TVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDRPDTLV 561
           ++KV  VS+  + +G L+HP L ++++KRW+KFS+S+DLPDL    R+  +TL+
Sbjct: 425 SLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQQQTREYEETLI 478

BLAST of Carg08880 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 702.2 bits (1811), Expect = 3.4e-202
Identity = 336/447 (75.17%), Postives = 396/447 (88.59%), Query Frame = 0

Query: 104 SPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEA 163
           SP+  VN  KK++VLPLVFLIFYEVSGGPFG+EDSV AAGPLLA++GF+VFP IWSIPEA
Sbjct: 42  SPARTVNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEA 101

Query: 164 LITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIP 223
           LITAEMGTMFPENGGYVVWV+ A+GP+WGFQQGW+KWLSGVIDNALYP+LFLDYLKS IP
Sbjct: 102 LITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIP 161

Query: 224 ALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSILPFAVMGLVSIPKLRPSR 283
            LG G+PRVAA+L LTV LTY+NYRGL+IVG  AV+LGVFSILPF VM  +SIPKL+PSR
Sbjct: 162 ILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSR 221

Query: 284 WLVVN--LKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLPKALFYALILVVLGYF 343
           WLVV+  +K V+W+LYLNTLFWNLNYWDS+STL GEVENP+KTLP+ALFYAL+LVV  Y 
Sbjct: 222 WLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYI 281

Query: 344 FPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAMSNMGMFVAEMSSDSFQ 403
           FP+L+GTGAI L++ LWTDGYF+D+ K+IGG WL WWI  AAA SNMGMF+AEMSSDSFQ
Sbjct: 282 FPVLTGTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQ 341

Query: 404 LLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIAAENFLYCFGMILE 463
           LLGMAERGMLPE F KRSR+ TP +GILFSASGV++LSWLSFQEI+AAEN LYCFGM+LE
Sbjct: 342 LLGMAERGMLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLE 401

Query: 464 FLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALSTVKVMIVSLAAVAVG 523
           F+ F++LR+K+PAASRP+KIPVG +GS+LMCIPPT+LI V++A + +KV +VSLAA+ +G
Sbjct: 402 FITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIG 461

Query: 524 LLLHPGLGYVEKKRWLKFSVSADLPDL 549
           L+L P L  VEKK WLKFS S+ LP+L
Sbjct: 462 LVLQPCLKQVEKKGWLKFSTSSHLPNL 488

BLAST of Carg08880 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 679.5 bits (1752), Expect = 2.3e-195
Identity = 337/472 (71.40%), Postives = 392/472 (83.05%), Query Frame = 0

Query: 89  AMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLAL 148
           A G L   +   VG S     N+ +KVS+LPLVFLIFYEVSGGPFG E SV AAGPLLAL
Sbjct: 10  AYGNLYDDDDGDVGGSSKEGNNSIQKVSMLPLVFLIFYEVSGGPFGAEGSVNAAGPLLAL 69

Query: 149 LGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 208
           LGF++FP IW IPEALITAEM TMFP NGG+VVWVSSALG FWGFQ GWMKWL GVIDNA
Sbjct: 70  LGFVIFPFIWCIPEALITAEMSTMFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNA 129

Query: 209 LYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSILPF 268
           LYPVLFLDYLKS +PAL  GLPRVA++L LT++LTY+NYRGLTIVGW AV +GVFS+LPF
Sbjct: 130 LYPVLFLDYLKSAVPALATGLPRVASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPF 189

Query: 269 AVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLPKA 328
           AVM LVSIP+L PSRWLV++L +V+WNLYLNTL WNLNYWDS+STLAGEV NP KTLPKA
Sbjct: 190 AVMSLVSIPQLEPSRWLVMDLGNVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKA 249

Query: 329 LFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWLSWWITGAAAMSNM 388
           L Y +I V L  F PLLSGTGAIPL+R+LWTDGY ++VAK IGG WL  W+  AAA SNM
Sbjct: 250 LCYGVIFVALSNFLPLLSGTGAIPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNM 309

Query: 389 GMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEIIA 448
           GMF+AEMSSDSFQLLGMAE G+LPE F +RSR+GTPL+GILFSASGV+LLS LSFQEIIA
Sbjct: 310 GMFLAEMSSDSFQLLGMAELGILPEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIA 369

Query: 449 AENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALSTV 508
           AEN LYC GMILEF+AF++LR KHPAASRPYKIPVGTVGSIL+C+PP +LIC+V+ LST+
Sbjct: 370 AENLLYCGGMILEFIAFVRLRKKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTI 429

Query: 509 KVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDRPDTLV 561
           KV +VS   V +G L+ P L +++ K+W+KFSV +DL +    N D  ++L+
Sbjct: 430 KVALVSFVMVVIGFLMKPCLNHMDGKKWVKFSVCSDLAEFQKENLDCEESLL 481

BLAST of Carg08880 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 543.5 bits (1399), Expect = 2.0e-154
Identity = 268/452 (59.29%), Postives = 340/452 (75.22%), Query Frame = 0

Query: 90  MGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGVEDSV-GAAGPLLAL 149
           MGE    E   V +  SS    + K+++LPLVFLIFYEVSGGPFGVEDSV    GPLLAL
Sbjct: 1   MGE----EETIVNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLAL 60

Query: 150 LGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 209
           LGFL+FPLIWSIPEAL+TAE+ T FPENGGYVVW+SSA GPFWGFQ+G+ KW SGV+DNA
Sbjct: 61  LGFLIFPLIWSIPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNA 120

Query: 210 LYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGWVAVILGVFSILPF 269
           LYPVLFLDYLK   P L     RV A+L +T  LTY+NYRGL IVG+ AV+L VFS+ PF
Sbjct: 121 LYPVLFLDYLKHSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPF 180

Query: 270 AVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLAGEVENPNKTLPKA 329
            VM L+++P +RP RWL V+ + ++W  Y NT+FWNLNYWD  STLAGEV+ P KT PKA
Sbjct: 181 VVMALLAVPNIRPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKA 240

Query: 330 LFYALILVVLGYFFPLLSGTGAIPLNRD-LWTDGYFSDVAKIIGGAWLSWWITGAAAMSN 389
           LF A++LV+  Y  PL++GTGA+  +    W+DGYF++V  +IGG WL  WI  AAAMSN
Sbjct: 241 LFGAVLLVMGSYLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSN 300

Query: 390 MGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGVVLLSWLSFQEII 449
           +G+F AEMSSD+FQLLGM+E GMLP FF +RS++GTP I IL SA+GV+ LSW+SFQEII
Sbjct: 301 LGLFEAEMSSDAFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEII 360

Query: 450 AAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPPTILICVVLALST 509
              NFLY  GM+LEF AF+KLRIK P   RPY++P+ T G  ++C+PP++L+ +V+ L+ 
Sbjct: 361 EFLNFLYALGMLLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAA 420

Query: 510 VKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKF 540
            K  ++S   + +G  L+P L  V++K+W +F
Sbjct: 421 PKTFLISGVIIVLGFCLYPFLTLVKEKQWARF 448