Homology
BLAST of Carg08824 vs. NCBI nr
Match:
KAG7036178.1 (Beta-amyrin 11-oxidase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1000.7 bits (2586), Expect = 4.3e-288
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF
Sbjct: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL
Sbjct: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE
Sbjct: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Carg08824 vs. NCBI nr
Match:
XP_022931256.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])
HSP 1 Score: 989.6 bits (2557), Expect = 9.9e-285
Identity = 484/490 (98.78%), Postives = 487/490 (99.39%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
MDIW+CLIGALAGVYVFLFGI RNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF
Sbjct: 3 MDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFTSKYGKTGMY+THLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYI HVEGIVVTCLEEWGSMKEPVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFH+GFMSTAVDIPGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFEKVLEEKRENLKNKV ENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Carg08824 vs. NCBI nr
Match:
XP_022995340.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])
HSP 1 Score: 989.2 bits (2556), Expect = 1.3e-284
Identity = 483/490 (98.57%), Postives = 488/490 (99.59%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
MDIW+CLIGALAGVYVF+FGI RNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF
Sbjct: 3 MDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFTSKYGKTGMY+THLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFEKVLEEKRENLKNKV ENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAHTIMRAIMFLSENPETLLKAKAEQE+IVNARPADAQHKGLTMKEIKQMEYLSKVMDE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPH+FDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Carg08824 vs. NCBI nr
Match:
XP_023534581.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 983.4 bits (2541), Expect = 7.1e-283
Identity = 483/490 (98.57%), Postives = 485/490 (98.98%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
MDI +CLIGALAGVYVFLFGI RNLNQWRFVTRH RRYNLPPGDMGWPLIGTLLPFLKAF
Sbjct: 3 MDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFTSKYGKTGMY+THLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFEKVLEEKRENLKNKV ENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAHTIMRAIMFLSENPETL KAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE
Sbjct: 303 TSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Carg08824 vs. NCBI nr
Match:
XP_023554757.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 912.9 bits (2358), Expect = 1.2e-261
Identity = 437/490 (89.18%), Postives = 469/490 (95.71%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
M++WV L+GAL G Y+F+F I RNLNQWRFVTRHG RYNLPPGDMGWPL+GTLLPFL+AF
Sbjct: 1 MEVWVWLVGALVGAYIFVFVILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF SKYGK GMY+T+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALN+V +PEHRR+RRLIASLISGN+ALS+YIGHVEGIV+TCLEEW +MK+PVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKT+AFK+LLHIFIGAN+AAFIDKMEKLY DFHLGFMS+AVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKRENLKNKVEENRKKDM DLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAH+IMRAIMFLSENPETL KAKAEQEQIVNARPA+ +H+GLTMKEIKQMEYLSKV+DE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQEQIVNARPANERHRGLTMKEIKQMEYLSKVIDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLP PTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Carg08824 vs. ExPASy Swiss-Prot
Match:
B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)
HSP 1 Score: 500.4 bits (1287), Expect = 2.4e-140
Identity = 246/490 (50.20%), Postives = 345/490 (70.41%), Query Frame = 0
Query: 4 WVCLIGA-LAGVYVFLFGIFRNLNQWRF-VTRHGRRYNLPPGDMGWPLIGTLLPFLKAFR 63
WVC+ A L Y+F RNLN W + V + + LPPGDMGWPLIG LL F+K F
Sbjct: 5 WVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFS 64
Query: 64 SGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRIL 123
SG PDSFI++ KYG++G+Y+THLFG+PS+IV P++CR VL +D F GY K+ + L
Sbjct: 65 SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKEL 124
Query: 124 TGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLS 183
+ + V EHR RRLI S I G++AL++Y+ +E IV+ LEE SMK PVE L
Sbjct: 125 ARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLK 184
Query: 184 EMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELI 243
EMK V+FK ++H+F+G+++ I K+ + D + G S +++PG TF +AL+AR +L
Sbjct: 185 EMKKVSFKAIVHVFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLA 244
Query: 244 RIFEKVLEEKRENLKNKVEE-NRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 303
+I + V++E+R ++N +E +++KD+ D+LLEV+DE+G L+D I DLLIG FAGHE
Sbjct: 245 KIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHE 304
Query: 304 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 363
++A ++M +I +L+++P L KAK EQE+I R + K L++KEIKQM YLS+V+DE
Sbjct: 305 STATSLMWSITYLTQHPHILKKAKEEQEEITRTR--FSSQKQLSLKEIKQMVYLSQVIDE 364
Query: 364 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 423
TLR ++AF RE DVN+NGY IPKGW++L+W RA+HM+ E Y NP +F+PSRWD+
Sbjct: 365 TLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDY 424
Query: 424 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 483
+AG+F+PFGAG RLCPG DL+KLEI+IFLHYF+ +RLER+NP C LP+ PTD
Sbjct: 425 NAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLR-NYRLERINPECHVTSLPVSKPTD 484
Query: 484 NCLARVVRDS 491
NCLA+V++ S
Sbjct: 485 NCLAKVIKVS 491
BLAST of Carg08824 vs. ExPASy Swiss-Prot
Match:
O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 458.8 bits (1179), Expect = 7.9e-128
Identity = 227/470 (48.30%), Postives = 324/470 (68.94%), Query Frame = 0
Query: 21 IFRNLNQWRFVTRHG-RRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKT 80
+ R +N W + + G R+ LPPGD+GWP IG +L FL+AF++ PDSF +YG
Sbjct: 25 LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84
Query: 81 GMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNSVPKPEHRRLR 140
G+Y+ H+FG+PS+IVT + CR VL +D+ F G+ +T L G K+ + EH+RLR
Sbjct: 85 GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144
Query: 141 RLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKVLLHIFIGAN 200
RL A+ ++G+EALS YI ++E V+T L++W M E EFL+ ++ + F+++++IF+ +
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204
Query: 201 SAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKV 260
S +D +E+ Y + G + AV+IPG + RALKAR L+ F+ ++ E+R K +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264
Query: 261 EENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPET 320
N KKDM D LL V+DEDG+ LDD IID+L+ + AGHE+S HTIM A +FL E+PE
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324
Query: 321 LLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDV 380
L +AKAEQE I+ +RP KGL++KE ++ME+LS+V+DETLR + + T RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384
Query: 381 NLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
+NGY IPKGWK+L W R VH++PE++ +P KFDP+RWDN PK AG+F+PFGAG L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444
Query: 441 CPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARV 487
CPG DL+KLEI+IFLH+F+ ++++R NP CP +LP PTDNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of Carg08824 vs. ExPASy Swiss-Prot
Match:
Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)
HSP 1 Score: 451.8 bits (1161), Expect = 9.7e-126
Identity = 226/487 (46.41%), Postives = 330/487 (67.76%), Query Frame = 0
Query: 3 IWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHG-RRYNLPPGDMGWPLIGTLLPFLKAFR 62
+W LI + G++V L + + +N W +V++ G +++ LPPGD+GWP+IG + FL+AF+
Sbjct: 9 MWFPLI--ILGLFV-LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68
Query: 63 SGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRIL 122
+ P+SFI + ++YG+TG+Y+ H+FG P V+VT PE CR VL +D+ F G+ K+T L
Sbjct: 69 TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128
Query: 123 TGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLS 182
G K+ + EH+RLRRL ++ ++G EALS+YI +E V T LE+W M E +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188
Query: 183 EMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELI 242
++ + FKV+++IF+ + S +D +E+ Y + + G + +++PG + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248
Query: 243 RIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHET 302
F+ ++ +R K + NR KDM D L++V+DE+G LDD IIDLL+ + AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308
Query: 303 SAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDET 362
S H M A + + E+P L KAK EQE+IV R A + LT+KE ++M YLS+V+DET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368
Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPK 422
LR + + T RE K DV ++GY IPKGWK+L W R VH++PEIY +P KFDPSRW+
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428
Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDN 482
+AG+F+PFG G LCPG DL+KLEI+IFLH+F+ +R+ER NP CP LP P DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486
Query: 483 CLARVVR 489
CLAR+ R
Sbjct: 489 CLARITR 486
BLAST of Carg08824 vs. ExPASy Swiss-Prot
Match:
Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 446.0 bits (1146), Expect = 5.3e-124
Identity = 216/464 (46.55%), Postives = 309/464 (66.59%), Query Frame = 0
Query: 28 WRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKTGMYRTHLF 87
W GRR LPPGDMGWPL+G + FL+AF+SGRPDSFI F ++G+ G+YR +F
Sbjct: 33 WTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPDSFIDSFARRFGRAGLYRAFMF 92
Query: 88 GSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNSVPKPEHRRLRRLIASLIS 147
SP+++ T PE C+ VLM+D+ FV G+ KAT L G K+ ++ EHRRLR+L A+ I+
Sbjct: 93 SSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRLRKLTAAPIN 152
Query: 148 GNEALSLYIGHVEGIVVTCLEEWGSM--KEPVEFLSEMKTVAFKVLLHIFIGANSAAFID 207
G +AL+ Y+G ++ VVT L W EFL+E++ + F++++ IF+G
Sbjct: 153 GFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFMGGADERTAA 212
Query: 208 KMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKR-ENLKNKVEENRK 267
++E+ Y + + G + A+D+PG + +A++AR L+ ++VL+E+R K
Sbjct: 213 ELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRARGGKTAAGAAAP 272
Query: 268 KDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPETLLKAK 327
DM D L+ V DE G L D IID+L+ + AGHE+S H M A +FL ENPE L KAK
Sbjct: 273 VDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPEILAKAK 332
Query: 328 AEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDVNLNGY 387
AEQE I+ + P KGLT+++ ++M YLS+V+DETLR +++F R+ DV +NGY
Sbjct: 333 AEQEAIMRSIPPG--QKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFRQATRDVFVNGY 392
Query: 388 TIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSK 447
IPKGWK+ +W R+VHM+P++Y +P KFDPSRW+ P RAG+F+PFG G RLCPG DL+K
Sbjct: 393 LIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLGTRLCPGNDLAK 452
Query: 448 LEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARVVR 489
LEI++FLH+F+ G++L R NPNC +LP P P DNCLA++ R
Sbjct: 453 LEISVFLHHFLL-GYKLTRKNPNCRVRYLPHPRPVDNCLAKITR 493
BLAST of Carg08824 vs. ExPASy Swiss-Prot
Match:
Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)
HSP 1 Score: 442.2 bits (1136), Expect = 7.7e-123
Identity = 213/492 (43.29%), Postives = 325/492 (66.06%), Query Frame = 0
Query: 2 DIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHG--RRYNLPPGDMGWPLIGTLLPFLKA 61
D+WV +AGV V + + R + W V G RR LPPG+MGWP++G++ FL+A
Sbjct: 16 DLWVLAAAVVAGV-VLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRA 75
Query: 62 FRSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATR 121
F+SG PD+FI F ++G+TG+YRT +F SP+++ PE C+ VLM+DE FV G+ KAT
Sbjct: 76 FKSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135
Query: 122 ILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKE-PVE 181
L G K+ ++ +HRR+R+L A+ I+G +AL+ Y+ ++ VV L W S + VE
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195
Query: 182 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 241
FL+E++ + FK+++ IF+ A ++ +E+ Y D + G + A+++PG + RAL+AR
Sbjct: 196 FLTELRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARR 255
Query: 242 ELIRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 301
+L+ + + VL+ +R + + DM D L+E DE G L D I+D+LI + AG
Sbjct: 256 KLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAG 315
Query: 302 HETSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 361
HE+S H M A +FL ENP+ +AKAEQE+I+ + P A GLT+++ K+M +LS+V+
Sbjct: 316 HESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIP--ATQNGLTLRDFKKMHFLSQVV 375
Query: 362 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 421
DETLR +++F R+ D+ +NGY IPKGWK+ +W R+VHM+ ++Y +P F+PSRW+
Sbjct: 376 DETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 435
Query: 422 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 481
P +AG+F+PFG G RLCPG DL+KLEI++FLH+F+ G++L+R NP C +LP P P
Sbjct: 436 GPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLKRANPKCRVRYLPHPRP 495
Query: 482 TDNCLARVVRDS 491
DNCLA + + S
Sbjct: 496 VDNCLATITKVS 503
BLAST of Carg08824 vs. ExPASy TrEMBL
Match:
A0A6J1ET68 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 SV=1)
HSP 1 Score: 989.6 bits (2557), Expect = 4.8e-285
Identity = 484/490 (98.78%), Postives = 487/490 (99.39%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
MDIW+CLIGALAGVYVFLFGI RNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF
Sbjct: 3 MDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFTSKYGKTGMY+THLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYI HVEGIVVTCLEEWGSMKEPVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFH+GFMSTAVDIPGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFEKVLEEKRENLKNKV ENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Carg08824 vs. ExPASy TrEMBL
Match:
A0A6J1K3T5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=1)
HSP 1 Score: 989.2 bits (2556), Expect = 6.2e-285
Identity = 483/490 (98.57%), Postives = 488/490 (99.59%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
MDIW+CLIGALAGVYVF+FGI RNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF
Sbjct: 3 MDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFTSKYGKTGMY+THLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFEKVLEEKRENLKNKV ENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAHTIMRAIMFLSENPETLLKAKAEQE+IVNARPADAQHKGLTMKEIKQMEYLSKVMDE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPH+FDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Carg08824 vs. ExPASy TrEMBL
Match:
A0A6J1I785 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=1)
HSP 1 Score: 911.4 bits (2354), Expect = 1.7e-261
Identity = 435/490 (88.78%), Postives = 468/490 (95.51%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
M++WV L+GAL G YVF+FGI RNLNQWRFVTRHG RYNLPPGDMGWPL+GTLLPFL+AF
Sbjct: 1 MEVWVWLVGALVGAYVFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF S+YGK GMY+T+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASQYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALN+V +PEHRR+RRLIASLISGN+ALS+YIGHVEGIV+TCLEEW +MK+PVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKT+AFK+LLHIFIGAN+AAFIDKMEKLY DFHLGFMS+AVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKRENLKNKVEENRKKDM DLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAH+IMRAIMFLSENPETL KAKAEQE+IVNARP +H+GLTMKEIKQMEYLSKV+DE
Sbjct: 301 TSAHSIMRAIMFLSENPETLCKAKAEQEKIVNARPTSERHRGLTMKEIKQMEYLSKVIDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHM+PEIYENP +FDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRTDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEEFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLP PTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Carg08824 vs. ExPASy TrEMBL
Match:
A0A6J1GM12 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 SV=1)
HSP 1 Score: 911.0 bits (2353), Expect = 2.2e-261
Identity = 435/490 (88.78%), Postives = 469/490 (95.71%), Query Frame = 0
Query: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
M++WV L+GAL G Y+ +FGI RNLNQWRFVTRHG RYNLPPGDMGWPL+GTLLPFL+AF
Sbjct: 1 MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF SKYGK GMY+T+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
LTGSKALN+V +PEHRR+RRLIASLISGN+ALS+YIGHVEGIV+TCLEEW +MK+PVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
SEMKT+AFK+LLHIFIGAN+AAFIDKMEKLY DFHLGFMS+AVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKRENLKNKVEENRKKDM DLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
TSAH+IMRAIMFLSENPETL KAKAEQ+QIVNARP++ +H+GLTMKEIKQMEYLSKV+DE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLP PTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 481 NCLARVVRDS 491
NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Carg08824 vs. ExPASy TrEMBL
Match:
A0A5A7TAG0 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00740 PE=3 SV=1)
HSP 1 Score: 910.2 bits (2351), Expect = 3.7e-261
Identity = 443/488 (90.78%), Postives = 464/488 (95.08%), Query Frame = 0
Query: 3 IWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAFRS 62
+W+ L+GAL Y+F+F I RNLNQW FVTRHGRRYNLPPGDMGWPLIGTLLPFL+AFRS
Sbjct: 4 LWLSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQAFRS 63
Query: 63 GRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILT 122
GRPDSFIH F KYGK GMY+T+LFGSPSVIVTVPEVCRHVLMNDEQF FGYSKATRILT
Sbjct: 64 GRPDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATRILT 123
Query: 123 GSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSE 182
GSKALN+VPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEW SMK+PVEFLSE
Sbjct: 124 GSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEFLSE 183
Query: 183 MKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIR 242
MKTVAFKVLLHIFIGAN+AAFID+MEKLYNDFHLGFMS+ VD+PGTTFSRALKARNELI+
Sbjct: 184 MKTVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNELIK 243
Query: 243 IFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETS 302
IFE VL+EKRENLK KV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHETS
Sbjct: 244 IFENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETS 303
Query: 303 AHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETL 362
AH+IMRAIMFLSENPETL KAKAEQEQIV ARPADAQHKGLTMKEIKQMEYL KV+DETL
Sbjct: 304 AHSIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDETL 363
Query: 363 RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKR 422
RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRW+NPKR
Sbjct: 364 RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWENPKR 423
Query: 423 RAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNC 482
R GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLP PTDNC
Sbjct: 424 RGGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNC 483
Query: 483 LARVVRDS 491
LARVVRDS
Sbjct: 484 LARVVRDS 491
BLAST of Carg08824 vs. TAIR 10
Match:
AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )
HSP 1 Score: 458.8 bits (1179), Expect = 5.6e-129
Identity = 227/470 (48.30%), Postives = 324/470 (68.94%), Query Frame = 0
Query: 21 IFRNLNQWRFVTRHG-RRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKT 80
+ R +N W + + G R+ LPPGD+GWP IG +L FL+AF++ PDSF +YG
Sbjct: 25 LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84
Query: 81 GMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNSVPKPEHRRLR 140
G+Y+ H+FG+PS+IVT + CR VL +D+ F G+ +T L G K+ + EH+RLR
Sbjct: 85 GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144
Query: 141 RLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKVLLHIFIGAN 200
RL A+ ++G+EALS YI ++E V+T L++W M E EFL+ ++ + F+++++IF+ +
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204
Query: 201 SAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKV 260
S +D +E+ Y + G + AV+IPG + RALKAR L+ F+ ++ E+R K +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264
Query: 261 EENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPET 320
N KKDM D LL V+DEDG+ LDD IID+L+ + AGHE+S HTIM A +FL E+PE
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324
Query: 321 LLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDV 380
L +AKAEQE I+ +RP KGL++KE ++ME+LS+V+DETLR + + T RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384
Query: 381 NLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
+NGY IPKGWK+L W R VH++PE++ +P KFDP+RWDN PK AG+F+PFGAG L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444
Query: 441 CPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARV 487
CPG DL+KLEI+IFLH+F+ ++++R NP CP +LP PTDNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of Carg08824 vs. TAIR 10
Match:
AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 451.8 bits (1161), Expect = 6.9e-127
Identity = 226/487 (46.41%), Postives = 330/487 (67.76%), Query Frame = 0
Query: 3 IWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHG-RRYNLPPGDMGWPLIGTLLPFLKAFR 62
+W LI + G++V L + + +N W +V++ G +++ LPPGD+GWP+IG + FL+AF+
Sbjct: 9 MWFPLI--ILGLFV-LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68
Query: 63 SGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRIL 122
+ P+SFI + ++YG+TG+Y+ H+FG P V+VT PE CR VL +D+ F G+ K+T L
Sbjct: 69 TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128
Query: 123 TGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLS 182
G K+ + EH+RLRRL ++ ++G EALS+YI +E V T LE+W M E +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188
Query: 183 EMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELI 242
++ + FKV+++IF+ + S +D +E+ Y + + G + +++PG + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248
Query: 243 RIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHET 302
F+ ++ +R K + NR KDM D L++V+DE+G LDD IIDLL+ + AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308
Query: 303 SAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDET 362
S H M A + + E+P L KAK EQE+IV R A + LT+KE ++M YLS+V+DET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368
Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPK 422
LR + + T RE K DV ++GY IPKGWK+L W R VH++PEIY +P KFDPSRW+
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428
Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDN 482
+AG+F+PFG G LCPG DL+KLEI+IFLH+F+ +R+ER NP CP LP P DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486
Query: 483 CLARVVR 489
CLAR+ R
Sbjct: 489 CLARITR 486
BLAST of Carg08824 vs. TAIR 10
Match:
AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 451.8 bits (1161), Expect = 6.9e-127
Identity = 226/487 (46.41%), Postives = 330/487 (67.76%), Query Frame = 0
Query: 3 IWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHG-RRYNLPPGDMGWPLIGTLLPFLKAFR 62
+W LI + G++V L + + +N W +V++ G +++ LPPGD+GWP+IG + FL+AF+
Sbjct: 9 MWFPLI--ILGLFV-LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68
Query: 63 SGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRIL 122
+ P+SFI + ++YG+TG+Y+ H+FG P V+VT PE CR VL +D+ F G+ K+T L
Sbjct: 69 TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128
Query: 123 TGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLS 182
G K+ + EH+RLRRL ++ ++G EALS+YI +E V T LE+W M E +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188
Query: 183 EMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELI 242
++ + FKV+++IF+ + S +D +E+ Y + + G + +++PG + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248
Query: 243 RIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHET 302
F+ ++ +R K + NR KDM D L++V+DE+G LDD IIDLL+ + AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308
Query: 303 SAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDET 362
S H M A + + E+P L KAK EQE+IV R A + LT+KE ++M YLS+V+DET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368
Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPK 422
LR + + T RE K DV ++GY IPKGWK+L W R VH++PEIY +P KFDPSRW+
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428
Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDN 482
+AG+F+PFG G LCPG DL+KLEI+IFLH+F+ +R+ER NP CP LP P DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486
Query: 483 CLARVVR 489
CLAR+ R
Sbjct: 489 CLARITR 486
BLAST of Carg08824 vs. TAIR 10
Match:
AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )
HSP 1 Score: 236.9 bits (603), Expect = 3.5e-62
Identity = 135/424 (31.84%), Postives = 228/424 (53.77%), Query Frame = 0
Query: 34 HGRRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVI 93
HG ++ P G +GWP+IG + F+ + S RP+SF+ YG+ ++++H+FG+ +++
Sbjct: 47 HGPKF--PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGR--VFKSHIFGTATIV 106
Query: 94 VTVPEVCRHVLMNDE-QFVFGYSKATRILTGSKALNSVPKPEHRRLRRLIASLISGNEAL 153
T EV R VL +D FV Y K R L G ++ + HRR L+ S +
Sbjct: 107 STDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLK 166
Query: 154 SLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYN 213
+ + + + ++ W S +PV KTVAFKVL I ++++++ +
Sbjct: 167 AQIVRDMHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFE 226
Query: 214 DFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKVEEN-RKKDMADLL 273
+F G MS ++ PGT R+L+A+ +++ E+++E K KNK E++ KD+ D+L
Sbjct: 227 NFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVL 286
Query: 274 LEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPETLLKAKAEQEQIV 333
L+ + E L I + +I GH++ I A+ FLS++P L E ++
Sbjct: 287 LK---DSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLK 346
Query: 334 NARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWK 393
+ + + + L + + + KV+ ETLR ++ + R+ DV + GY IPKGW
Sbjct: 347 SLK--ELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWC 406
Query: 394 ILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFL 453
L + R+VH++ YE+P+KF+P RW SF PFG G RLCPG+DL++LE ++FL
Sbjct: 407 FLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFL 460
Query: 454 HYFI 456
H+ +
Sbjct: 467 HHLV 460
BLAST of Carg08824 vs. TAIR 10
Match:
AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 226.1 bits (575), Expect = 6.1e-59
Identity = 148/453 (32.67%), Postives = 233/453 (51.43%), Query Frame = 0
Query: 36 RRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVT 95
+R LPPG MG P IG L + P+SF +KYG +++TH+ G P V+++
Sbjct: 45 QRLRLPPGSMGLPYIGETL----RLYTENPNSFFATRQNKYG--DIFKTHILGCPCVMIS 104
Query: 96 VPEVCRHVLMNDEQ-FVFGYSKATRILTGSKALNSVPKPEHRRLRRLIASLISGNEALSL 155
PE R VL++ F Y + + G +AL P H L+RL+ S + AL
Sbjct: 105 SPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPS-ALRP 164
Query: 156 YIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKV-LLHIFIGANSAAFIDKMEKLYND 215
+ H+E +V+ L W S K + L MK AF V ++ F ID ++ LY
Sbjct: 165 TVSHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQR 224
Query: 216 FHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKVEENRKKDMADLLLE 275
G+ S +D+PGT F +++KAR EL KV+E++REN + + +LL
Sbjct: 225 LERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRREN------GREEGGLLGVLLG 284
Query: 276 VRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPETLLKAKAEQEQIVNA 335
+D+ GL D I D +IG FA +T+A + + +L ++P L + EQ I
Sbjct: 285 AKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSI--R 344
Query: 336 RPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKIL 395
+ +++ ++ ++ ++M ++V+ ETLR S+ RE DV +GY IPKGWK+L
Sbjct: 345 QKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVL 404
Query: 396 VWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHY 455
R +H + E + +P KFDPSR++ + +++PFG G+ CPG +L+KLE+ I LH+
Sbjct: 405 PLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHH 464
Query: 456 FIHGGFRLERVNPNCPENHLPLPTPTDNCLARV 487
+ FR E + + P P P RV
Sbjct: 465 -LTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRV 479
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
B5BSX1 | 2.4e-140 | 50.20 | Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1 | [more] |
O23051 | 7.9e-128 | 48.30 | Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1 | [more] |
Q9C5Y2 | 9.7e-126 | 46.41 | Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2 | [more] |
Q9AXH9 | 5.3e-124 | 46.55 | Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1 | [more] |
Q5VRM7 | 7.7e-123 | 43.29 | Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1ET68 | 4.8e-285 | 98.78 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 S... | [more] |
A0A6J1K3T5 | 6.2e-285 | 98.57 | beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=... | [more] |
A0A6J1I785 | 1.7e-261 | 88.78 | beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=... | [more] |
A0A6J1GM12 | 2.2e-261 | 88.78 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 S... | [more] |
A0A5A7TAG0 | 3.7e-261 | 90.78 | Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... | [more] |