Carg08815 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08815
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	Carg_Chr02: 8928111 .. 8928626 (+)
RNA-Seq Expression	Carg08815
Synteny	Carg08815

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTTTTCTCTTCTAACCCTCTTCCAATTTGGGTTCTGTTTGGACATTGTTCAACGCTCCTGCAAACTAGCTGCAAAAAGAGACCCATACGTTGATTACAAGCTGTGTGTTAAGAGCCTACGAGCCAACCCCAGTTCAAAAGATGCAACGTTCAAGGATTTGGTCCTGATTTCGATCGAGCAATCGAAGGCTAATGCGACGGCAATTGGGTCGGAGATTACGGGGCTCTTAAAGGGCGGTGGGAAATGGGGGAAGTATTCGACGAGTTGTTTGGAGAGCTGTTTGGAGGTTTATTCTGAGGCTGTTTCGGATTTGAAATTGGGTTTGGTTGCTGTCAAAATGGGGGATTATGAAACTGCAAATACAGAGGTGAGTGCCGCCATGGATGCACCTTTGACTTGTGAAGATGGGTATAATGAGAAAGATGGGGAAGTTTCTCCTTTGACAGAGAAGAACGGTGGGTTCTTTCAGTTGACTGCGATTTCTCTGGCTTTTATCAGTTTGTGTCAGTGA

mRNA sequence

ATGCTTTTTTCTCTTCTAACCCTCTTCCAATTTGGGTTCTGTTTGGACATTGTTCAACGCTCCTGCAAACTAGCTGCAAAAAGAGACCCATACGTTGATTACAAGCTGTGTGTTAAGAGCCTACGAGCCAACCCCAGTTCAAAAGATGCAACGTTCAAGGATTTGGTCCTGATTTCGATCGAGCAATCGAAGGCTAATGCGACGGCAATTGGGTCGGAGATTACGGGGCTCTTAAAGGGCGGTGGGAAATGGGGGAAGTATTCGACGAGTTGTTTGGAGAGCTGTTTGGAGGTTTATTCTGAGGCTGTTTCGGATTTGAAATTGGGTTTGGTTGCTGTCAAAATGGGGGATTATGAAACTGCAAATACAGAGGTGAGTGCCGCCATGGATGCACCTTTGACTTGTGAAGATGGGTATAATGAGAAAGATGGGGAAGTTTCTCCTTTGACAGAGAAGAACGGTGGGTTCTTTCAGTTGACTGCGATTTCTCTGGCTTTTATCAGTTTGTGTCAGTGA

Coding sequence (CDS)

ATGCTTTTTTCTCTTCTAACCCTCTTCCAATTTGGGTTCTGTTTGGACATTGTTCAACGCTCCTGCAAACTAGCTGCAAAAAGAGACCCATACGTTGATTACAAGCTGTGTGTTAAGAGCCTACGAGCCAACCCCAGTTCAAAAGATGCAACGTTCAAGGATTTGGTCCTGATTTCGATCGAGCAATCGAAGGCTAATGCGACGGCAATTGGGTCGGAGATTACGGGGCTCTTAAAGGGCGGTGGGAAATGGGGGAAGTATTCGACGAGTTGTTTGGAGAGCTGTTTGGAGGTTTATTCTGAGGCTGTTTCGGATTTGAAATTGGGTTTGGTTGCTGTCAAAATGGGGGATTATGAAACTGCAAATACAGAGGTGAGTGCCGCCATGGATGCACCTTTGACTTGTGAAGATGGGTATAATGAGAAAGATGGGGAAGTTTCTCCTTTGACAGAGAAGAACGGTGGGTTCTTTCAGTTGACTGCGATTTCTCTGGCTTTTATCAGTTTGTGTCAGTGA

Protein sequence

MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ

Homology

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BLAST of Carg08815 vs. NCBI nr
Match: KAG6606222.1 (hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036169.1 hypothetical protein SDJN02_02970, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.6 bits (867), Expect = 3.2e-89
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60
           MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI
Sbjct: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60

Query: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120
           EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET
Sbjct: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120

Query: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 172
           ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ
Sbjct: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 171

[Close]

BLAST of Carg08815 vs. NCBI nr
Match: XP_022958457.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 337.4 bits (864), Expect = 7.1e-89
Identity = 170/171 (99.42%), Postives = 171/171 (100.00%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60
           MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCV+SLRANPSSKDATFKDLVLISI
Sbjct: 11  MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSKDATFKDLVLISI 70

Query: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120
           EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET
Sbjct: 71  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 130

Query: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 172
           ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ
Sbjct: 131 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 181

[Close]

BLAST of Carg08815 vs. NCBI nr
Match: XP_022995887.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 329.7 bits (844), Expect = 1.5e-86
Identity = 167/171 (97.66%), Postives = 170/171 (99.42%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60
           +LFSLLTLFQFGF LDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI
Sbjct: 11  VLFSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 70

Query: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120
           EQSKANATAIGSEITGLLKGGGKWGKYS+SCLESCLEVYSEAVSDLKLGLVAVKMGDYET
Sbjct: 71  EQSKANATAIGSEITGLLKGGGKWGKYSSSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 130

Query: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 172
           ANTEVSAAMDAPLTCE+GYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ
Sbjct: 131 ANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 181

[Close]

BLAST of Carg08815 vs. NCBI nr
Match: XP_023534693.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.6 bits (841), Expect = 3.3e-86
Identity = 167/171 (97.66%), Postives = 169/171 (98.83%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60
           +L SLLTLFQFGF LDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI
Sbjct: 11  VLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 70

Query: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120
           EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET
Sbjct: 71  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 130

Query: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 172
           ANTEVSAAMDAPLTCE+GYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ
Sbjct: 131 ANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 181

[Close]

BLAST of Carg08815 vs. NCBI nr
Match: XP_023523101.1 (putative invertase inhibitor, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291.2 bits (744), Expect = 5.8e-75
Identity = 145/147 (98.64%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 11  FGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAI 70
           FGF LDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAI
Sbjct: 1   FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAI 60

Query: 71  GSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMD 130
           GSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMD
Sbjct: 61  GSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMD 120

Query: 131 APLTCEDGYNEKDGEVSPLTEKNGGFF 158
           APLTCE+GYNEKDGEVSPLTEKNGGFF
Sbjct: 121 APLTCENGYNEKDGEVSPLTEKNGGFF 147

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BLAST of Carg08815 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 7.5e-25
Identity = 69/167 (41.32%), Postives = 98/167 (58.68%), Query Frame = 0


Query: 3   FSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQ 62
           F+LL L Q     DIVQ +CK  A+R P V+Y  CVKSL A+P S  A  + L +IS   
Sbjct: 12  FNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANL 71

Query: 63  SKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETAN 122
           +  + + I + I  +LK   K        L  C+ +Y++A S ++  +   K  DY +AN
Sbjct: 72  AIQHGSKIQTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASAN 131

Query: 123 TEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 170
            ++SAA+D  +TCEDG+ EK G VSP+T++N  + QLTAISLA   L
Sbjct: 132 VKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of Carg08815 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.0e-23
Identity = 67/167 (40.12%), Postives = 94/167 (56.29%), Query Frame = 0


Query: 3   FSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQ 62
           FSL   F      DIVQ +CK  A+R P V+Y  CVKSL A+P S  A  + L +IS   
Sbjct: 5   FSLCIFFLIS--ADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANL 64

Query: 63  SKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETAN 122
           +    + I + I  +LK   K        L  C+ +Y++A S ++  +   K  DY +AN
Sbjct: 65  AIQQGSKIQTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASAN 124

Query: 123 TEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 170
            ++SAA+D  +TCEDG+ EK G  SP+T++N  + QLTAISLA   L
Sbjct: 125 VKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of Carg08815 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.4e-17
Identity = 62/172 (36.05%), Postives = 90/172 (52.33%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLVLIS 60
           ++FSL  L  F     ++Q SCK A  +DP + Y  CV SL  +P SK A T + LVL S
Sbjct: 8   VIFSLF-LNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLAS 67

Query: 61  IEQSKANATAIGSEITGLLKGGGKWGKYS--TSCLESCLEVYSEAVSDLKLGLVAVKMGD 120
            + + A  T     + G++    K  +Y      L+ CL  Y++A  DL   L  +K  D
Sbjct: 68  TKTAAAKIT----NLKGIVAQDLKDQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSRD 127

Query: 121 YETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 170
           Y+ AN  +SAA+D P  CED + E   + SP+T +N   F+   I LAF ++
Sbjct: 128 YQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Carg08815 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 3.5e-06
Identity = 36/122 (29.51%), Postives = 58/122 (47.54%), Query Frame = 0


Query: 37  CVKSLRANPSSKDATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCL 96
           C   +++NP +  A  + L  I+   ++ +A+    +I  L+K              SC+
Sbjct: 43  CTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTA--TNPTMKKAYTSCV 102

Query: 97  EVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGF 156
           + Y  A+S L     ++  GD +  N +VSAAM+ P TCE   +  D +V P   KN G 
Sbjct: 103 QHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQ--DMADFKVDPSAVKNSGD 160

Query: 157 FQ 159
           FQ
Sbjct: 163 FQ 160

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BLAST of Carg08815 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.6e-04
Identity = 35/144 (24.31%), Postives = 60/144 (41.67%), Query Frame = 0


Query: 34  YKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYST---- 93
           Y +CV +L A+PSS  A  + L  I++  + ANA+        L  G       +     
Sbjct: 72  YDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALANGTAPLAAAAAGDGT 131

Query: 94  --SCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVS 153
             + L +C   Y +A   L     ++   DY+ A   VSA  + P  C+  +  +     
Sbjct: 132 VQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYPQVCKALFRRQRPGAY 191

Query: 154 P--LTEKNGGFFQLTAISLAFISL 170
           P  L  +     +L +++L  I+L
Sbjct: 192 PAELAAREEALNKLCSVALDIIAL 215

[Close]

BLAST of Carg08815 vs. ExPASy TrEMBL
Match: A0A6J1H1W1 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 3.4e-89
Identity = 170/171 (99.42%), Postives = 171/171 (100.00%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60
           MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCV+SLRANPSSKDATFKDLVLISI
Sbjct: 11  MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSKDATFKDLVLISI 70

Query: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120
           EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET
Sbjct: 71  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 130

Query: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 172
           ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ
Sbjct: 131 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 181

[Close]

BLAST of Carg08815 vs. ExPASy TrEMBL
Match: A0A6J1K084 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111491280 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 7.2e-87
Identity = 167/171 (97.66%), Postives = 170/171 (99.42%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60
           +LFSLLTLFQFGF LDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI
Sbjct: 11  VLFSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 70

Query: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120
           EQSKANATAIGSEITGLLKGGGKWGKYS+SCLESCLEVYSEAVSDLKLGLVAVKMGDYET
Sbjct: 71  EQSKANATAIGSEITGLLKGGGKWGKYSSSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 130

Query: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 172
           ANTEVSAAMDAPLTCE+GYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ
Sbjct: 131 ANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 181

[Close]

BLAST of Carg08815 vs. ExPASy TrEMBL
Match: A0A6J1ICI2 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.5e-60
Identity = 127/171 (74.27%), Postives = 142/171 (83.04%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60
           +L SL T F   F LDI+QRSCK AAK DPYVDYKLCV++LRANP+SK ATFK+LVLISI
Sbjct: 12  LLLSLPTPFHLAFSLDIIQRSCKSAAKTDPYVDYKLCVQTLRANPNSKAATFKELVLISI 71

Query: 61  EQSKANATAIGSEITGLLKGGGKW--GKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDY 120
            QSKANAT IGSEI+ LLKG  +W  GKYS  CL+ CLE+YSEAVS L+L L AVKMGDY
Sbjct: 72  TQSKANATEIGSEISELLKGSERWGKGKYSLGCLKGCLEIYSEAVSGLELALGAVKMGDY 131

Query: 121 ETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 170
           ETAN +VSAAMDA L+CEDGY EKDGEVSPLTE N GFFQL AISLAFI++
Sbjct: 132 ETANIKVSAAMDAALSCEDGYKEKDGEVSPLTEINCGFFQLNAISLAFINM 182

[Close]

BLAST of Carg08815 vs. ExPASy TrEMBL
Match: A0A0A0LHE9 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 5.3e-58
Identity = 120/170 (70.59%), Postives = 141/170 (82.94%), Query Frame = 0


Query: 2   LFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIE 61
           L  + TL Q  + LDIVQ SCKLAAK DPYVDYKLCV++L+A+P+SKDA FKDLV+ISI 
Sbjct: 12  LLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPNSKDAEFKDLVVISIN 71

Query: 62  QSKANATAIGSEITGLLK-GGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 121
           QSKANAT IGSEI+ L+K    KWG+YS +CL+SCLE+YSEAVSDL+  L  +KM DYET
Sbjct: 72  QSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEKALKGLKMEDYET 131

Query: 122 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 171
           A T VSAAMDAP++CEDGY EKDGEVSPL+E N GFFQL AISLAFI++C
Sbjct: 132 AKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQLVAISLAFINMC 181

[Close]

BLAST of Carg08815 vs. ExPASy TrEMBL
Match: A0A6J1E2X6 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111025985 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.2e-57
Identity = 121/179 (67.60%), Postives = 141/179 (78.77%), Query Frame = 0


Query: 1   MLFSLLTLFQ--FGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLI 60
           +LFSLL+L Q       D+VQRSCK AA RDPYVDYKLCV++LRANP +++  FKDLVL+
Sbjct: 11  LLFSLLSLCQSESAPANDVVQRSCKQAATRDPYVDYKLCVEALRANPKARNGAFKDLVLV 70

Query: 61  SIEQSKANATAIGSEITGLLKGGGKWG-------KYSTSCLESCLEVYSEAVSDLKLGLV 120
           SI+Q+KANAT IGSEI+ +LKG GKWG       KYS  CL SCLE+YSEAVSDLK  + 
Sbjct: 71  SIDQAKANATEIGSEISEILKGSGKWGTKPKTKTKYSLDCLRSCLELYSEAVSDLKSAVS 130

Query: 121 AVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 171
           AVKM DYETA  EVSAA+DAP++CEDGY EKDGEVSPLTEKN  FF L AISLAF+ +C
Sbjct: 131 AVKMEDYETAKVEVSAAIDAPVSCEDGYKEKDGEVSPLTEKNDVFFHLAAISLAFVDMC 189

[Close]

BLAST of Carg08815 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.0e-21
Identity = 56/154 (36.36%), Postives = 84/154 (54.55%), Query Frame = 0


Query: 17  IVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLVLISIEQSKANATAIGSEIT 76
           +++ SCK A    P   Y LCV SL  NP +K A     LV+ S + +   AT +   + 
Sbjct: 23  LIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLVMASTKNAVTKATTLKGTVD 82

Query: 77  GLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMDAPLTC 136
            ++K G K  K +   L  CL++Y++A+  L   L  VK  +Y T  T +SAAMD P TC
Sbjct: 83  KIIK-GKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRNYPTVKTVLSAAMDTPSTC 142

Query: 137 EDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 170
           E G+ E+    SP+T++N   +Q+  I LAF ++
Sbjct: 143 ETGFKERKAP-SPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of Carg08815 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.0e-21
Identity = 63/159 (39.62%), Postives = 88/159 (55.35%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKD-LVLIS 60
           M F LL  F       ++Q  CK AA ++P + Y  CVKSL  NP SK A   D LV++S
Sbjct: 9   MFFLLLNAFT--IAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLS 68

Query: 61  IEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYE 120
            + + +  T++   +  +LK   ++  YS   L  CLE+YS+A + LK  L  +K  DY+
Sbjct: 69  TKNAVSKTTSMKGIVDKILK-ENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSRDYK 128

Query: 121 TANTEVSAAMDAPLTCEDGYNE-KDGEVSPLTEKNGGFF 158
           TAN  +SAAM AP +CE G+ E K    SP T+ N   F
Sbjct: 129 TANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Carg08815 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 1.5e-20
Identity = 64/172 (37.21%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLVLIS 60
           M F LL    F     +++ SCK A  +DP + Y  CVKSL  NP SK A + + LV +S
Sbjct: 9   MFFLLLNC--FATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLVFVS 68

Query: 61  IEQSKANATAIGSEITGLLKGGGKWGKYSTS-CLESCLEVYSEAVSDLKLGLVAVKMGDY 120
            + + +  T++   +  +LK      KY     L  CLE+Y+EA+  L   L  VK  DY
Sbjct: 69  TKNAVSKTTSLKGMVDKILKE----DKYEVERPLLDCLELYTEAIDSLNQSLDTVKSRDY 128

Query: 121 ETANTEVSAAMDAPLTCEDGYNEKDGEV-SPLTEKNGGFFQLTAISLAFISL 170
           +TA   +SAAMDAP +CE  + ++   V SP T++N   F +  I +AF S+
Sbjct: 129 KTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of Carg08815 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.1e-18
Identity = 62/172 (36.05%), Postives = 90/172 (52.33%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLVLIS 60
           ++FSL  L  F     ++Q SCK A  +DP + Y  CV SL  +P SK A T + LVL S
Sbjct: 8   VIFSLF-LNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLAS 67

Query: 61  IEQSKANATAIGSEITGLLKGGGKWGKYS--TSCLESCLEVYSEAVSDLKLGLVAVKMGD 120
            + + A  T     + G++    K  +Y      L+ CL  Y++A  DL   L  +K  D
Sbjct: 68  TKTAAAKIT----NLKGIVAQDLKDQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSRD 127

Query: 121 YETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 170
           Y+ AN  +SAA+D P  CED + E   + SP+T +N   F+   I LAF ++
Sbjct: 128 YQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Carg08815 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 9.1e-18
Identity = 61/172 (35.47%), Postives = 91/172 (52.91%), Query Frame = 0


Query: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLVLIS 60
           ++FSL  L  F     ++Q SCK A  +DP   Y  CV+SL  +P SK A T +DL L S
Sbjct: 8   VIFSLF-LNGFATAQTLIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATTLEDLALAS 67

Query: 61  IEQSKANATAIGSEITGLLKGGGKWGKYS--TSCLESCLEVYSEAVSDLKLGLVAVKMGD 120
            +   A  T     + G++    K  +Y      L+ CL  Y +A   LK  L  +K  D
Sbjct: 68  TKNVAAKIT----NLKGIVAQDLKDQRYQDIVEDLKLCLGFYKDANDSLKTALANIKSRD 127

Query: 121 YETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 170
           Y+ AN+ +SAA++AP  CED + E + + SP+T +N   ++   I LAF ++
Sbjct: 128 YDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLAFTTM 173

The following BLAST results are available for this feature:

BLAST of Carg08815 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606222.1	3.2e-89	100.00	hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022958457.1	7.1e-89	99.42	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_022995887.1	1.5e-86	97.66	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_023534693.1	3.3e-86	97.66	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
XP_023523101.1	5.8e-75	98.64	putative invertase inhibitor, partial [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg08815 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	7.5e-25	41.32	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	7.0e-23	40.12	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	4.4e-17	36.05	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
Q9LUV1	3.5e-06	29.51	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
Q0J8J8	5.6e-04	24.31	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of Carg08815 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H1W1	3.4e-89	99.42	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 ...	[more]
A0A6J1K084	7.2e-87	97.66	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111491280 PE=4 SV...	[more]
A0A6J1ICI2	1.5e-60	74.27	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV...	[more]
A0A0A0LHE9	5.3e-58	70.59	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 S...	[more]
A0A6J1E2X6	1.2e-57	67.60	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111025985 PE=4...	[more]

BLAST of Carg08815 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46940.1	1.0e-21	36.36	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	3.0e-21	39.62	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	1.5e-20	37.21	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	3.1e-18	36.05	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	9.1e-18	35.47	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 13..166 e-value: 5.5E-25 score: 99.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 16..168 e-value: 1.2E-31 score: 107.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 17..165 e-value: 4.4E-27 score: 95.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 15..169 e-value: 6.9E-34 score: 119.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 16..168
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 10..169
None	No IPR available	PANTHER	PTHR31080:SF239	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 10..169
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 22..169 e-value: 9.41324E-41 score: 132.489

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08815-RA	Carg08815-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity