Carg08788 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08788
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	palmitoyl-protein thioesterase 1-like
Location	Carg_Chr02: 8774543 .. 8777666 (-)
RNA-Seq Expression	Carg08788
Synteny	Carg08788

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGGTTTTATAATTTGGCGTTGTTATTTCTCATTCTCAACAGCCAATGTTGTGCGAGGGAACTCCGTAATTTCATCGCCCCATCTTCCTTCTGTCTGCAAATTCCTACTAACACTCTCCAACTAATGGCGTTTTCTTCACTGCCCATCATCTTCTTCTTCCTTTTCGTTTCAATAATCATCCCAATTTCTCATTCCATACCCTTCATAGTGCTCCACGGTATGTATCTTCTTCCATTCTTGACTTTCTTCCCCTCTTTTTCTGTTTCTATTTTAATCCAACTATCTTCTTTTGTATCCGATGATTCCTTTATGTTTATTATCAACTGGGTGTGTTTAAATTCTGCCGATTCAACTTGTTGGATGAAGAAATCGATTGTTGGGTATCGATAATTTTTGTTTCCTCGAAAGGGTATTTCCGAGGGATGTTTGGATGCTGAAATTTGTTTTCTGGAAATTACTGTTCCTGGTTGTTTGATTCTAGGTCTACAGAGATTCATATCTGTTCTTGGAGGAATATTTGAGACCCCCCACCCCTGTATTTTGTTTCTCTGGAATCTGTAGGTTTCAGCTTCTAACCCACTTCTTGATTTGTTTTTTCTTTATGGTTTATCCAGCTGATCCCATTAAGTTTATGAGTTTGATTGAGTTGCAATGTTGGTCAGGGTTCAATTGGAGCTATTTAAAGTGGCCTGTCGTGTTTTAAAAGTTTCTTGAGTTGATATTTTCTCTCAATTGCAGGTATTGGAGATAAATGCTCAAATAAAGGCATGAGGAAGTTTACAAAGTACTTGAGTGATAATTCTGGTGCTGAGGGATATTGCATGTATGCTTGTCCGAAGATCTGTATCCATTTTCATTACTAACTTGTTATAAGCTTGCTCTGATGATATACTAGCATGTTATAAGCTGCCATTATGTTCATATTAGTTTGTTATAAGATTGCTCTCATGATTGACTGAGCTGCGAGCATTGATATTGCTTCATCCATCTTCCTACAGAGAAATTGGCAATGGGGGATGGGATTCGTGGTTTATGTCCCTTGAGGAGCAGGTTAGATAATGAGTAATATGATTCAATCATCTCGAGCTACTAAGAATTTTTTCAAGTTGTGATGTTTCTGTTTTATGTTTGGCAGTCCCAAATTGTTTGCAACAAGGTGGTATAGTTCTCCAGCTGGTTTTTGTTCCGATTGGATTTCGACATCGATGCTAACGTGCAACTAGTTCGTTATTATGACTACAGGTGAAGAAAATGAAAGAGCTAAAAGAGGGCTTCAACTTTGTTGGTCTCTCCCAGGTGATTCATGACGACTTGAGAACTATAATTCTGTAACTAACTTTAGTTTGTGTCTTGTCCAATCGTTTTGTTCTTGTTCTTGTTCTTGTTCCCCTTTCCAACTTTTCATAAAGTAGTAAAGATTAAGATAGTCTAACTCAGTCTGGGACACATTTGGTTCTCTTAATAGGGTAACTTAATCGGCCGAGCAGTCGTAGAATTCTGCGATGGAGCGCCTCCTGTGAGAACCACTTTGATTATTTACCTTCTAGATTGGTGTTTATCAGCCTGATATGTACTGAATCTAATTTGTTGCTTCTCTGTTCATGGGAATAGGTTAAGAACTTCATCTCTTTAGGCGGTCCTCATGCTGGCATAGCCTCTGTTCCACTTTGTGGTGTAAGAACTGTTCTTGTGTTCCATCTTCATGGGAATAGGTTACATTTTCTGTTGCTGAAGGCCTTGAATATATTTGCAGTCTGGCATTTGGTGCATACTTGCAGACGACATAATGAAGTTAGGTATCTACTCAGATGCTGTCCAAGTACGTCACCATATCCGAGCTTCCTTGAACTCTATGGTTTTGTGAAAATTGTCAACAAATCTGTTAGATCCCCACCTCGGTCGGTTGGGGAGGAGAACGAAACATTCTTTGTAAGGGTGTGGAAACCTCTCCCTAGAGGGGAAGTCCAAAGAGGACAAATATCTGCTAGCGGTGGACTTGGGCCGTTACAAAATCGAAGTCTTGACACGTTCTCTTATTATTGTCTTGCAGAATTCTTTGGCTCCTAGTGGTTATATTAAAATACCGAATGTGAGTTATATTTGTTTACAAACCCAGATATGATTTCTGTACATCTCCAAGTTCTCAATGATCCTTCTTTTCTTTTTCAGGATATCCCCAACTATTTAGAGCACTGTAAGTTTCTCCCGAAGCTTAATAACGAACGTCCCGATGCCAGAAACTCGACGTACAAGGAACGATTTAGTAGTTTAGAGAACTTGGTGCTAATAATGGTAAGTTTGATATGGAGAAGCTGATGCAAATTTATAGTGTTATTGTTTTGTCATATATTTTTGGTTTGGCTCACTGTTTCAATAGTTTGAGGATGATAAGGTTCTGGTCCCAAAGGAAACGGCGTGGTTCGGGTATTACCCGGACGGGGAGTTTGAGCCTGTTTTACCCCCACAGCAGGTACAACATTTTGGATGTCATAGCAATGCCAGATCTTGGCTAAGAATTGAAAAACACACAGCACACATACACGCACACGCACATGTTTTAGGCACGGTGATGTTGCGGTGTCTTAACACCATGATTGTTTTGGTGCAGACAAAGCTTTACATAGAGGATTGGATTGGATTGAGAAGGCTAGATGAAGGTGGAAGAGTCCAGTTTATAAAAGTTGAAGGAGGTCATCTTGGAATCTCGTACAGTGATGCAAAGAAGTACATTGTGCCTTATCTGGAGGAGGAACAGCAGCAACCTCTTCAATTTGGTTTACAAAGCAAGCATGTTTCTTGGAGTTTAGCTTACGGGAAGTAGTAGCTTTCAAGCTACAATCGAATACCATCAACAACCTTATAATATTCAAATACAGACAAAGCTTTTGCATTATTCATATTATAAAAATGTCCTTTTTAAGGATATTACTCGACTCTGCGCAATATTCATATCACTATTTTGAGGGCCAGCAATTTTGAGGTTCCAGTTGCCGGGCAGCTCCTGTTAGGAATCACGACTCTTTACAATTGTATGATATTGTCCTCTTTGAACATAAGTTCTCGTGGCTTTGTTTTGGGCTTCCCCAAAAAGCCTCATACAAATAGATATGGTATTCTCCACT

mRNA sequence

ATTGGTTTTATAATTTGGCGTTGTTATTTCTCATTCTCAACAGCCAATGTTGTGCGAGGGAACTCCGTAATTTCATCGCCCCATCTTCCTTCTGTCTGCAAATTCCTACTAACACTCTCCAACTAATGGCGTTTTCTTCACTGCCCATCATCTTCTTCTTCCTTTTCGTTTCAATAATCATCCCAATTTCTCATTCCATACCCTTCATAGTGCTCCACGGTATTGGAGATAAATGCTCAAATAAAGGCATGAGGAAGTTTACAAAGTACTTGAGTGATAATTCTGGTGCTGAGGGATATTGCATAGAAATTGGCAATGGGGGATGGGATTCGTGGTTTATGTCCCTTGAGGAGCAGTCCCAAATTGTTTGCAACAAGGTGGTGAAGAAAATGAAAGAGCTAAAAGAGGGCTTCAACTTTGTTGGTCTCTCCCAGGGTAACTTAATCGGCCGAGCAGTCGTAGAATTCTGCGATGGAGCGCCTCCTGTTAAGAACTTCATCTCTTTAGGCGGTCCTCATGCTGGCATAGCCTCTGTTCCACTTTGTGGTTCTGGCATTTGGTGCATACTTGCAGACGACATAATGAAGTTAGGTATCTACTCAGATGCTGTCCAAGTACGTCACCATATCCGAGCTTCCTTGAACTCTATGGTTTTGTGAAAATTGTCAACAAATCTGTTAGATCCCCACCTCGGTCGGTTGGGGAGGAGAACGAAACATTCTTTGTAAGGGTGTGGAAACCTCTCCCTAGAGGGGAAGTCCAAAGAGGACAAATATCTGCTAGCGGTGGACTTGGGCCGTTACAAAATCGAAGTCTTGACACGTTCTCTTATTATTGTCTTGCAGAATTCTTTGGCTCCTAGTGGTTATATTAAAATACCGAATGATATCCCCAACTATTTAGAGCACTGTAAGTTTCTCCCGAAGCTTAATAACGAACGTCCCGATGCCAGAAACTCGACGTACAAGGAACGATTTAGTAGTTTAGAGAACTTGGTGCTAATAATGTTTGAGGATGATAAGGTTCTGGTCCCAAAGGAAACGGCGTGGTTCGGGTATTACCCGGACGGGGAGTTTGAGCCTGTTTTACCCCCACAGCAGACAAAGCTTTACATAGAGGATTGGATTGGATTGAGAAGGCTAGATGAAGGTGGAAGAGTCCAGTTTATAAAAGTTGAAGGAGGTCATCTTGGAATCTCGTACAGTGATGCAAAGAAGTACATTGTGCCTTATCTGGAGGAGGAACAGCAGCAACCTCTTCAATTTGGTTTACAAAGCAAGCATGTTTCTTGGAGTTTAGCTTACGGGAAGTAGTAGCTTTCAAGCTACAATCGAATACCATCAACAACCTTATAATATTCAAATACAGACAAAGCTTTTGCATTATTCATATTATAAAAATGTCCTTTTTAAGGATATTACTCGACTCTGCGCAATATTCATATCACTATTTTGAGGGCCAGCAATTTTGAGGTTCCAGTTGCCGGGCAGCTCCTGTTAGGAATCACGACTCTTTACAATTGTATGATATTGTCCTCTTTGAACATAAGTTCTCGTGGCTTTGTTTTGGGCTTCCCCAAAAAGCCTCATACAAATAGATATGGTATTCTCCACT

Coding sequence (CDS)

ATGGCGTTTTCTTCACTGCCCATCATCTTCTTCTTCCTTTTCGTTTCAATAATCATCCCAATTTCTCATTCCATACCCTTCATAGTGCTCCACGGTATTGGAGATAAATGCTCAAATAAAGGCATGAGGAAGTTTACAAAGTACTTGAGTGATAATTCTGGTGCTGAGGGATATTGCATAGAAATTGGCAATGGGGGATGGGATTCGTGGTTTATGTCCCTTGAGGAGCAGTCCCAAATTGTTTGCAACAAGGTGGTGAAGAAAATGAAAGAGCTAAAAGAGGGCTTCAACTTTGTTGGTCTCTCCCAGGGTAACTTAATCGGCCGAGCAGTCGTAGAATTCTGCGATGGAGCGCCTCCTGTTAAGAACTTCATCTCTTTAGGCGGTCCTCATGCTGGCATAGCCTCTGTTCCACTTTGTGGTTCTGGCATTTGGTGCATACTTGCAGACGACATAATGAAGTTAGGTATCTACTCAGATGCTGTCCAAGTACGTCACCATATCCGAGCTTCCTTGAACTCTATGGTTTTGTGA

Protein sequence

MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCIEIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQVRHHIRASLNSMVL

Homology

[Close]

BLAST of Carg08788 vs. NCBI nr
Match: KAG7036142.1 (pdf1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 360.9 bits (925), Expect = 6.2e-96
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI
Sbjct: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQVRHHIRASLNSMVL 178
           VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQVRHHIRASLNSMVL
Sbjct: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQVRHHIRASLNSMVL 177

[Close]

BLAST of Carg08788 vs. NCBI nr
Match: XP_023533387.1 (palmitoyl-protein thioesterase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.2 bits (840), Expect = 4.5e-86
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI
Sbjct: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFMSLEEQSQIVCNK VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMSLEEQSQIVCNK-VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ
Sbjct: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 162

[Close]

BLAST of Carg08788 vs. NCBI nr
Match: XP_022995687.1 (palmitoyl-protein thioesterase 1-like [Cucurbita maxima])

HSP 1 Score: 328.2 bits (840), Expect = 4.5e-86
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI
Sbjct: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFMSLEEQSQIVCNK VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMSLEEQSQIVCNK-VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ
Sbjct: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 162

[Close]

BLAST of Carg08788 vs. NCBI nr
Match: KAG6606197.1 (Palmitoyl-protein thioesterase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 325.5 bits (833), Expect = 2.9e-85
Identity = 161/163 (98.77%), Postives = 161/163 (98.77%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MA SSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI
Sbjct: 1   MAVSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFMSLEEQSQIVCNK VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMSLEEQSQIVCNK-VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ
Sbjct: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 162

[Close]

BLAST of Carg08788 vs. NCBI nr
Match: XP_022958226.1 (palmitoyl-protein thioesterase 1-like [Cucurbita moschata])

HSP 1 Score: 320.5 bits (820), Expect = 9.3e-84
Identity = 159/163 (97.55%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MAFSSL II FFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGA+GYCI
Sbjct: 1   MAFSSLLIIIFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAKGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFMSLEEQSQIVCNK VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMSLEEQSQIVCNK-VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ
Sbjct: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 162

[Close]

BLAST of Carg08788 vs. ExPASy Swiss-Prot
Match: P45478 (Palmitoyl-protein thioesterase 1 OS=Bos taurus OX=9913 GN=PPT1 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.2e-13
Identity = 47/149 (31.54%), Postives = 76/149 (51.01%), Query Frame = 0


Query: 25  IPFIVLHGIGDKCSNKGMRKFTKYLSDNS--GAEGYCIEIG----NGGWDSWFMSLEEQS 84
           +P ++ HG+GD C N       K + +    G     +EIG        +S+F+++  Q 
Sbjct: 33  LPLVIWHGMGDSCCNPLSMGAIKKMVEKKIPGIHVLSLEIGKTLREDVENSFFLNVNSQV 92

Query: 85  QIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVP 144
             VC +++ K  +L++G+N +G SQG    RAV + C  +PP+ N IS+GG H G+  +P
Sbjct: 93  TTVC-QILAKDPKLQQGYNAMGFSQGGQFLRAVAQRCP-SPPMVNLISVGGQHQGVFGLP 152

Query: 145 LC--GSGIWCILADDIMKLGIYSDAVQVR 166
            C   S   C      +  G Y+ A+Q R
Sbjct: 153 RCPGESSHICDFIRKTLNAGAYNKAIQER 179

[Close]

BLAST of Carg08788 vs. ExPASy Swiss-Prot
Match: O88531 (Palmitoyl-protein thioesterase 1 OS=Mus musculus OX=10090 GN=Ppt1 PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 6.8e-13
Identity = 47/148 (31.76%), Postives = 74/148 (50.00%), Query Frame = 0


Query: 26  PFIVLHGIGDKCSNKGMRKFTKYLSDNS--GAEGYCIEIGNGGWD----SWFMSLEEQSQ 85
           P ++ HG+GD C N       K + +    G     +EIG    +    S+F+++  Q  
Sbjct: 34  PLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVN 93

Query: 86  IVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVPL 145
           +VC ++++K  +L++G+N +G SQG    RAV + C   PP+   IS+GG H G+  +P 
Sbjct: 94  MVC-QILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCP-TPPMMTLISVGGQHQGVFGLPR 153

Query: 146 C--GSGIWCILADDIMKLGIYSDAVQVR 166
           C   S   C      +  G YS  VQ R
Sbjct: 154 CPGESSHICDFIRKSLNAGAYSKLVQER 179

[Close]

BLAST of Carg08788 vs. ExPASy Swiss-Prot
Match: P50897 (Palmitoyl-protein thioesterase 1 OS=Homo sapiens OX=9606 GN=PPT1 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.9e-13
Identity = 49/149 (32.89%), Postives = 73/149 (48.99%), Query Frame = 0


Query: 25  IPFIVLHGIGDKCSNKGMRKFTKYLSDNS--GAEGYCIEIGNGGWD----SWFMSLEEQS 84
           +P ++ HG+GD C N       K + +    G     +EIG    +    S+F+++  Q 
Sbjct: 33  LPLVIWHGMGDSCCNPLSMGAIKKMVEKKIPGIYVLSLEIGKTLMEDVENSFFLNVNSQV 92

Query: 85  QIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVP 144
             VC  + K  K L++G+N +G SQG    RAV + C  +PP+ N IS+GG H G+  +P
Sbjct: 93  TTVCQALAKDPK-LQQGYNAMGFSQGGQFLRAVAQRCP-SPPMINLISVGGQHQGVFGLP 152

Query: 145 LC--GSGIWCILADDIMKLGIYSDAVQVR 166
            C   S   C      +  G YS  VQ R
Sbjct: 153 RCPGESSHICDFIRKTLNAGAYSKVVQER 179

[Close]

BLAST of Carg08788 vs. ExPASy Swiss-Prot
Match: Q8HXW6 (Palmitoyl-protein thioesterase 1 OS=Macaca fascicularis OX=9541 GN=PPT1 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.9e-13
Identity = 49/149 (32.89%), Postives = 73/149 (48.99%), Query Frame = 0


Query: 25  IPFIVLHGIGDKCSNKGMRKFTKYLSDNS--GAEGYCIEIGNGGWD----SWFMSLEEQS 84
           +P ++ HG+GD C N       K + +    G     +EIG    +    S+F+++  Q 
Sbjct: 33  LPLVIWHGMGDSCCNPLSMGAIKKMVEKKIPGIYVLSLEIGKTLMEDVENSFFLNVNSQV 92

Query: 85  QIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVP 144
             VC  + K  K L++G+N +G SQG    RAV + C  +PP+ N IS+GG H G+  +P
Sbjct: 93  TTVCQTLAKDPK-LQQGYNAMGFSQGGQFLRAVAQRCP-SPPMINLISVGGQHQGVFGLP 152

Query: 145 LC--GSGIWCILADDIMKLGIYSDAVQVR 166
            C   S   C      +  G YS  VQ R
Sbjct: 153 RCPGESSHICDFIRKTLNAGAYSKVVQER 179

[Close]

BLAST of Carg08788 vs. ExPASy Swiss-Prot
Match: O59747 (Palmitoyl-protein thioesterase-dolichyl pyrophosphate phosphatase fusion 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pdf1 PE=1 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 8.3e-11
Identity = 55/195 (28.21%), Postives = 97/195 (49.74%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFV-----SIIIPI--------------SHSIPFIVLHGIGDKCSNKG 60
           ++ SS  I F F +V     S  IPI              S  +P ++ HG+GD  ++  
Sbjct: 2   LSCSSFLIFFLFSWVLLPMKSFAIPIISLDKVRLAINDGASEQLPVVIWHGLGDTPTSFT 61

Query: 61  MRKFTKYLSDNSGAEGYCIEIGNGGWD----SWFMSLEEQSQIVCNKVVKKMKELKEGFN 120
           + + ++ +   +    Y I +G+  ++     +   LE+Q   VC+ ++     L  GF 
Sbjct: 62  LTEVSQRVQKLTKGAVYAIRVGDNEFEDIKAGYLGKLEDQLDEVCD-LIGNEDSLSNGFY 121

Query: 121 FVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVPLCG-SGIWC-ILADDIMKL 171
            +GLSQG L  RA+ + CD A  +++ I+LG PH+GI ++P C  + + C  +   I+ L
Sbjct: 122 ALGLSQGGLFLRALAQTCDAA-KIRSLITLGSPHSGINTIPGCSPTNLICKAVVHSILGL 181

[Close]

BLAST of Carg08788 vs. ExPASy TrEMBL
Match: A0A6J1JZN3 (palmitoyl-protein thioesterase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111491146 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.2e-86
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI
Sbjct: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFMSLEEQSQIVCNK VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMSLEEQSQIVCNK-VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ
Sbjct: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 162

[Close]

BLAST of Carg08788 vs. ExPASy TrEMBL
Match: A0A6J1H1J8 (palmitoyl-protein thioesterase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459515 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.5e-84
Identity = 159/163 (97.55%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MAFSSL II FFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGA+GYCI
Sbjct: 1   MAFSSLLIIIFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAKGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFMSLEEQSQIVCNK VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMSLEEQSQIVCNK-VKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ
Sbjct: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 162

[Close]

BLAST of Carg08788 vs. ExPASy TrEMBL
Match: A0A1S3AT74 (palmitoyl-protein thioesterase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482587 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 7.9e-81
Identity = 146/163 (89.57%), Postives = 157/163 (96.32%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MA SSLPI FFF+FV IIIPISHS+PFIVLHGIGDKCSNKGMRKFTKYLSDNSG++GYCI
Sbjct: 1   MALSSLPIFFFFVFVFIIIPISHSLPFIVLHGIGDKCSNKGMRKFTKYLSDNSGSKGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFM+LE QSQIVCNKVVKKMK+LKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMTLEAQSQIVCNKVVKKMKDLKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           V+NFISLGGPHAGI+SVPLCGSGIWC+LADD++KL IYSD VQ
Sbjct: 121 VQNFISLGGPHAGISSVPLCGSGIWCLLADDLLKLAIYSDVVQ 163

[Close]

BLAST of Carg08788 vs. ExPASy TrEMBL
Match: A0A1S3AT23 (palmitoyl-protein thioesterase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482587 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.7e-79
Identity = 145/163 (88.96%), Postives = 156/163 (95.71%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MA SSLPI FFF+FV IIIPISHS+PFIVLHGIGDKCSNKGMRKFTKYLSDNSG++GYCI
Sbjct: 1   MALSSLPIFFFFVFVFIIIPISHSLPFIVLHGIGDKCSNKGMRKFTKYLSDNSGSKGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNGGWDSWFM+LE QSQIVCNK VKKMK+LKEGFNFVGLSQGNLIGRAVVEFCDGAPP
Sbjct: 61  EIGNGGWDSWFMTLEAQSQIVCNK-VKKMKDLKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           V+NFISLGGPHAGI+SVPLCGSGIWC+LADD++KL IYSD VQ
Sbjct: 121 VQNFISLGGPHAGISSVPLCGSGIWCLLADDLLKLAIYSDVVQ 162

[Close]

BLAST of Carg08788 vs. ExPASy TrEMBL
Match: A0A6J1E438 (palmitoyl-protein thioesterase 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025895 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.6e-73
Identity = 134/163 (82.21%), Postives = 151/163 (92.64%), Query Frame = 0


Query: 1   MAFSSLPIIFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCI 60
           MAFSSLPIIFF LF+S+I PISHSIPF+VLHGIGDKCS++G+++FTKYLSDNSGAEGYCI
Sbjct: 1   MAFSSLPIIFFLLFISLISPISHSIPFVVLHGIGDKCSHRGVKQFTKYLSDNSGAEGYCI 60

Query: 61  EIGNGGWDSWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPP 120
           EIGNG WDSWFM LE+Q+QIVC+K VK+MK L++GFN VGLSQGNLIGR VVEFCDGAPP
Sbjct: 61  EIGNGPWDSWFMPLEKQTQIVCDK-VKEMKVLRKGFNIVGLSQGNLIGRGVVEFCDGAPP 120

Query: 121 VKNFISLGGPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           V NFISLGGPHAG ASVPLCGSGIWCILADD++KL +YSD VQ
Sbjct: 121 VNNFISLGGPHAGTASVPLCGSGIWCILADDLLKLAVYSDVVQ 162

[Close]

BLAST of Carg08788 vs. TAIR 10
Match: AT3G60340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 7.7e-52
Identity = 89/143 (62.24%), Postives = 116/143 (81.12%), Query Frame = 0


Query: 21  ISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCIEIGNGGWDSWFMSLEEQSQI 80
           ++ S+PFIVLHGIGDKCSN G+ +FT+ LSD SG++GYC+EIGNG WDSW M L +Q+ +
Sbjct: 19  LASSVPFIVLHGIGDKCSNAGVTQFTELLSDWSGSQGYCLEIGNGSWDSWTMPLLDQTSV 78

Query: 81  VCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVPLC 140
           VC K VK M EL +G++ VGLSQGN+IGRA++EFCDGAPPVK+F+S+ GPHAG AS+P C
Sbjct: 79  VCEK-VKSMPELSDGYSIVGLSQGNMIGRALIEFCDGAPPVKSFVSVAGPHAGTASIPFC 138

Query: 141 GSGIWCILADDIMKLGIYSDAVQ 164
           G+   CI+ D ++K  IYSD +Q
Sbjct: 139 GATWICIMLDSMIKAEIYSDYMQ 160

[Close]

BLAST of Carg08788 vs. TAIR 10
Match: AT3G60340.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 7.7e-52
Identity = 89/143 (62.24%), Postives = 116/143 (81.12%), Query Frame = 0


Query: 21  ISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCIEIGNGGWDSWFMSLEEQSQI 80
           ++ S+PFIVLHGIGDKCSN G+ +FT+ LSD SG++GYC+EIGNG WDSW M L +Q+ +
Sbjct: 19  LASSVPFIVLHGIGDKCSNAGVTQFTELLSDWSGSQGYCLEIGNGSWDSWTMPLLDQTSV 78

Query: 81  VCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASVPLC 140
           VC K VK M EL +G++ VGLSQGN+IGRA++EFCDGAPPVK+F+S+ GPHAG AS+P C
Sbjct: 79  VCEK-VKSMPELSDGYSIVGLSQGNMIGRALIEFCDGAPPVKSFVSVAGPHAGTASIPFC 138

Query: 141 GSGIWCILADDIMKLGIYSDAVQ 164
           G+   CI+ D ++K  IYSD +Q
Sbjct: 139 GATWICIMLDSMIKAEIYSDYMQ 160

[Close]

BLAST of Carg08788 vs. TAIR 10
Match: AT5G47330.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 5.7e-47
Identity = 84/146 (57.53%), Postives = 113/146 (77.40%), Query Frame = 0


Query: 18  IIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCIEIGNGGWDSWFMSLEEQ 77
           ++ +S S+PFI+LHGI  +CSN     FT+ L++ SG+ G+C+EIGNG  DSW M L  Q
Sbjct: 20  MVHVSVSVPFIMLHGISAQCSNARDANFTQLLTNLSGSPGFCLEIGNGVADSWLMPLTRQ 79

Query: 78  SQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASV 137
           ++I C K VK+MKEL +G+N VG SQGNL+ R ++EFCDG PPV N+ISL GPHAGI+SV
Sbjct: 80  AEIACEK-VKQMKELSQGYNIVGRSQGNLVARGLIEFCDGGPPVYNYISLAGPHAGISSV 139

Query: 138 PLCGSGIWCILADDIMKLGIYSDAVQ 164
           P+CGSG++C LAD+++K  IYSD +Q
Sbjct: 140 PMCGSGLFCKLADELIKGDIYSDFIQ 164

[Close]

BLAST of Carg08788 vs. TAIR 10
Match: AT4G17483.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.1e-45
Identity = 84/146 (57.53%), Postives = 109/146 (74.66%), Query Frame = 0


Query: 18  IIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCIEIGNGGWDSWFMSLEEQ 77
           +IP+S SIPFI+ HGIGDKCS  G+  FT+ L++ SG+ GYC+EIGNG  DSWFM L +Q
Sbjct: 22  LIPVSISIPFILFHGIGDKCSG-GVSNFTQLLTNLSGSPGYCLEIGNGEKDSWFMPLRKQ 81

Query: 78  SQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLGGPHAGIASV 137
           + + C K VK MK L +G+N V  SQGNL+ R ++EFCD APPV N+ISLGGPHAGIA +
Sbjct: 82  TSVACEK-VKAMKVLSQGYNIVAESQGNLVARGLIEFCDNAPPVINYISLGGPHAGIAKI 141

Query: 138 PLCGSGIWCILADDIMKLGIYSDAVQ 164
           P C SG  C + + ++KL +Y+D VQ
Sbjct: 142 PKCNSGPICAIGEALLKLEVYTDFVQ 165

[Close]

BLAST of Carg08788 vs. TAIR 10
Match: AT4G17480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 1.7e-38
Identity = 78/155 (50.32%), Postives = 107/155 (69.03%), Query Frame = 0


Query: 9   IFFFLFVSIIIPISHSIPFIVLHGIGDKCSNKGMRKFTKYLSDNSGAEGYCIEIGNGGWD 68
           +FFF      IP+S S+PFI+ HGI D+CSN G+  F + LS+ S + G C+EIGNG  D
Sbjct: 16  LFFF------IPVSISVPFILFHGIRDQCSNGGVSSFVQLLSNLSSSHGSCLEIGNGEQD 75

Query: 69  SWFMSLEEQSQIVCNKVVKKMKELKEGFNFVGLSQGNLIGRAVVEFCDGAPPVKNFISLG 128
           S  M L +Q+ + C K VK+MK+L +G+N V  SQGNL+ R ++EFCD APPV N+ISLG
Sbjct: 76  SVTMPLTQQASVACEK-VKQMKDLSQGYNIVAQSQGNLVARGLIEFCDNAPPVFNYISLG 135

Query: 129 GPHAGIASVPLCGSGIWCILADDIMKLGIYSDAVQ 164
           GPHAGI+ +P C S + C    +++K  +Y+D VQ
Sbjct: 136 GPHAGISDIPKCNSTL-C----ELLKTAVYTDFVQ 158

The following BLAST results are available for this feature:

BLAST of Carg08788 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036142.1	6.2e-96	100.00	pdf1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023533387.1	4.5e-86	99.39	palmitoyl-protein thioesterase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022995687.1	4.5e-86	99.39	palmitoyl-protein thioesterase 1-like [Cucurbita maxima]	[more]
KAG6606197.1	2.9e-85	98.77	Palmitoyl-protein thioesterase 1, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022958226.1	9.3e-84	97.55	palmitoyl-protein thioesterase 1-like [Cucurbita moschata]	[more]

BLAST of Carg08788 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P45478	5.2e-13	31.54	Palmitoyl-protein thioesterase 1 OS=Bos taurus OX=9913 GN=PPT1 PE=1 SV=1	[more]
O88531	6.8e-13	31.76	Palmitoyl-protein thioesterase 1 OS=Mus musculus OX=10090 GN=Ppt1 PE=1 SV=2	[more]
P50897	8.9e-13	32.89	Palmitoyl-protein thioesterase 1 OS=Homo sapiens OX=9606 GN=PPT1 PE=1 SV=1	[more]
Q8HXW6	8.9e-13	32.89	Palmitoyl-protein thioesterase 1 OS=Macaca fascicularis OX=9541 GN=PPT1 PE=2 SV=...	[more]
O59747	8.3e-11	28.21	Palmitoyl-protein thioesterase-dolichyl pyrophosphate phosphatase fusion 1 OS=Sc...	[more]

BLAST of Carg08788 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JZN3	2.2e-86	99.39	palmitoyl-protein thioesterase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149114...	[more]
A0A6J1H1J8	4.5e-84	97.55	palmitoyl-protein thioesterase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459...	[more]
A0A1S3AT74	7.9e-81	89.57	palmitoyl-protein thioesterase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S3AT23	5.7e-79	88.96	palmitoyl-protein thioesterase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1E438	3.6e-73	82.21	palmitoyl-protein thioesterase 1-like isoform X1 OS=Momordica charantia OX=3673 ...	[more]

BLAST of Carg08788 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G60340.1	7.7e-52	62.24	alpha/beta-Hydrolases superfamily protein	[more]
AT3G60340.2	7.7e-52	62.24	alpha/beta-Hydrolases superfamily protein	[more]
AT5G47330.1	5.7e-47	57.53	alpha/beta-Hydrolases superfamily protein	[more]
AT4G17483.1	1.1e-45	57.53	alpha/beta-Hydrolases superfamily protein	[more]
AT4G17480.1	1.7e-38	50.32	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02089	Palm_thioest	coord: 26..165 e-value: 1.9E-30 score: 106.5
None	No IPR available	PANTHER	PTHR11247:SF58	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 6..164
None	No IPR available	PANTHER	PTHR11247	PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1	coord: 6..164
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 20..173 e-value: 2.6E-41 score: 143.8
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 22..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08788-RA	Carg08788-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098734	macromolecule depalmitoylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0098599	palmitoyl hydrolase activity