Carg08742 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08742
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calmodulin
Location	Carg_Chr09: 750569 .. 751123 (+)
RNA-Seq Expression	Carg08742
Synteny	Carg08742

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCTTTCACCCATTTCCACTCCCTTCAAATGGTTTTCCAACACCAATCTCAAGCTCAACCTCCCTCGCCTTCGCTTCAAACATAAGCCCACCCGGTCTAACCCTCCTCCTCCACCACTACTCCTCCTCACGCCTGCGCCCCCGCTCGCCGAAGCTGCCTTCCGCCACCTCGATGCCGATGGAGACGGCAAAATCTCAGGGGACGAGCTCCAATCCTACTTTGCATCGGTCGGTGAGTACATGACACGGGACAATGCTCGAAGTCTGATAAGAGAGTTCGACGGGGATGGTGACTTGTTGCTGGAGCTCGGAGACTTCGAACGGCTGGTGAAGGGCGGGGAGGAGGAAGAGAAGGAGGATCTGAAAAGGGCTTTTGAGATGTTTGAAGAAGAAAAGGGATGCGGATTCATCGGACCGGCAGGGCTGCAAAAGATGTTTGGACGGCTTGGATACGTGAAATCCATAGAAGAGTGCATGGCTATGATCGGAGTGTTTGATGTTGACGGCGACGGTGTCATTGATTATCGTGAATTTCTCAGAATGATGACTTAA

mRNA sequence

ATGGCCCTTTCACCCATTTCCACTCCCTTCAAATGGTTTTCCAACACCAATCTCAAGCTCAACCTCCCTCGCCTTCGCTTCAAACATAAGCCCACCCGGTCTAACCCTCCTCCTCCACCACTACTCCTCCTCACGCCTGCGCCCCCGCTCGCCGAAGCTGCCTTCCGCCACCTCGATGCCGATGGAGACGGCAAAATCTCAGGGGACGAGCTCCAATCCTACTTTGCATCGGTCGGTGAGTACATGACACGGGACAATGCTCGAAGTCTGATAAGAGAGTTCGACGGGGATGGTGACTTGTTGCTGGAGCTCGGAGACTTCGAACGGCTGGTGAAGGGCGGGGAGGAGGAAGAGAAGGAGGATCTGAAAAGGGCTTTTGAGATGTTTGAAGAAGAAAAGGGATGCGGATTCATCGGACCGGCAGGGCTGCAAAAGATGTTTGGACGGCTTGGATACGTGAAATCCATAGAAGAGTGCATGGCTATGATCGGAGTGTTTGATGTTGACGGCGACGGTGTCATTGATTATCGTGAATTTCTCAGAATGATGACTTAA

Coding sequence (CDS)

ATGGCCCTTTCACCCATTTCCACTCCCTTCAAATGGTTTTCCAACACCAATCTCAAGCTCAACCTCCCTCGCCTTCGCTTCAAACATAAGCCCACCCGGTCTAACCCTCCTCCTCCACCACTACTCCTCCTCACGCCTGCGCCCCCGCTCGCCGAAGCTGCCTTCCGCCACCTCGATGCCGATGGAGACGGCAAAATCTCAGGGGACGAGCTCCAATCCTACTTTGCATCGGTCGGTGAGTACATGACACGGGACAATGCTCGAAGTCTGATAAGAGAGTTCGACGGGGATGGTGACTTGTTGCTGGAGCTCGGAGACTTCGAACGGCTGGTGAAGGGCGGGGAGGAGGAAGAGAAGGAGGATCTGAAAAGGGCTTTTGAGATGTTTGAAGAAGAAAAGGGATGCGGATTCATCGGACCGGCAGGGCTGCAAAAGATGTTTGGACGGCTTGGATACGTGAAATCCATAGAAGAGTGCATGGCTATGATCGGAGTGTTTGATGTTGACGGCGACGGTGTCATTGATTATCGTGAATTTCTCAGAATGATGACTTAA

Protein sequence

MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMMT

Homology

[Close]

BLAST of Carg08742 vs. NCBI nr
Match: KAG7024217.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374.4 bits (960), Expect = 5.6e-100
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL
Sbjct: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180

Query: 181 RMMT 185
           RMMT
Sbjct: 181 RMMT 184

[Close]

BLAST of Carg08742 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.8 bits (948), Expect = 1.4e-98
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDELQSYFASVGEYMTRD+ARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLKRAFEMFE EKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180

Query: 181 RMMT 185
           RMMT
Sbjct: 181 RMMT 184

[Close]

BLAST of Carg08742 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 361.3 bits (926), Expect = 4.9e-96
Identity = 177/184 (96.20%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MALSP+STPFKWFSNTN  LN PRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA
Sbjct: 38  MALSPVSTPFKWFSNTNFNLNFPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 97

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDELQSYFASVGEYMTRD+ARSLIREFD DGDLLLELGDFERLVKGGEEEEKE
Sbjct: 98  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKGGEEEEKE 157

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLKRAFEMFE EKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL
Sbjct: 158 DLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 217

Query: 181 RMMT 185
           RMMT
Sbjct: 218 RMMT 221

[Close]

BLAST of Carg08742 vs. NCBI nr
Match: XP_022936314.1 (probable calcium-binding protein CML41 [Cucurbita moschata])

HSP 1 Score: 360.5 bits (924), Expect = 8.4e-96
Identity = 179/184 (97.28%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPP LLLTPAPPLAEAAFRHLDA
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPP-LLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDELQSYFASVGEYMTRD+ARSLIREFDGDGDLLLELGDFERLVKGGE+EEKE
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEQEEKE 120

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLKRAFEMFE EKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDG+GVIDYREFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFL 180

Query: 181 RMMT 185
           RMMT
Sbjct: 181 RMMT 183

[Close]

BLAST of Carg08742 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 359.8 bits (922), Expect = 1.4e-95
Identity = 177/184 (96.20%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MALSPISTPFKWFSNTN KLN PRLRFKHKPT SNPPPPPLLLLTPAPPLAEAAFRHLDA
Sbjct: 15  MALSPISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDA 74

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDELQSYFASVGEYMTRD+ARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE
Sbjct: 75  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 134

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLK AFEMFE EKGCGFIGP GLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL
Sbjct: 135 DLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 194

Query: 181 RMMT 185
           RMMT
Sbjct: 195 RMMT 198

[Close]

BLAST of Carg08742 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 84/197 (42.64%), Postives = 105/197 (53.30%), Query Frame = 0


Query: 5   PISTPFKWFSNTNLKLNLPRLRFKHKP----------TRSNPPPPPLLLLTPA-PPLAEA 64
           P S  FKWFS   LKLNL     +  P           RSN      +    A       
Sbjct: 8   PSSNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQ 67

Query: 65  AFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVK- 124
            F H D+DGDGKIS  EL+ YF SVGEY++ + A+  I E D D D  L   DF  L+  
Sbjct: 68  VFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTR 127

Query: 125 -----GGEEEEKEDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFD 184
                 GE +   +LK AFEMFE EKG G I P GLQKM  +LG  ++  EC AMI  +D
Sbjct: 128 RDLYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYD 187

[Close]

BLAST of Carg08742 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.6e-20
Identity = 53/133 (39.85%), Postives = 85/133 (63.91%), Query Frame = 0


Query: 52  EAAFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLV 111
           EA F ++DA+ DG+IS +ELQ  F ++GE ++ + A + +R  D DGD +L+  +F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 112 KGGEEEEKE-DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDG 171
           K  +EEEK+ +LK AF ++  E G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 172 DGVIDYREFLRMM 184
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Carg08742 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.0e-19
Identity = 51/136 (37.50%), Postives = 83/136 (61.03%), Query Frame = 0


Query: 52  EAAFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLV 111
           EA F ++DA+ DG+IS +EL+  F ++GE M+ + A + ++  D DGD +L++ +F  L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 112 KGG----EEEEKEDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFD 171
           KG     EEE+K  +  AF M+  + G   I P  L+ M  +LG  ++ ++C  MI  FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIAD-GEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 172 VDGDGVIDYREFLRMM 184
           ++ DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

[Close]

BLAST of Carg08742 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 8.7e-19
Identity = 60/134 (44.78%), Postives = 77/134 (57.46%), Query Frame = 0


Query: 55  FRHLDADGDGKISGDELQSYF-ASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLV-- 114
           F   D D DGKIS  EL+    A++GE M+ + A +L+   D D D LL+  +F +L   
Sbjct: 12  FSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEFTKLAVQ 71

Query: 115 --KGGEEEEKEDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVD 174
              G EEE    L  AF M+E E G G I PA L++M  +LG  + IEEC  MI  FD+D
Sbjct: 72  LEMGDEEERCRGLMEAFRMYEME-GEGRITPASLKRMLSKLGSHQGIEECQTMICRFDLD 131

Query: 175 GDGVIDYREFLRMM 184
           GDGVI + EF  MM
Sbjct: 132 GDGVISFEEFKIMM 144

[Close]

BLAST of Carg08742 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.5e-18
Identity = 59/143 (41.26%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 47  APPLAEAAFRHLDADGDGKISGDELQSYF-ASVGEYMTRDNARSLIREFDGDGDLLLELG 106
           +P  + A F   D DGDG+IS  EL+     ++GE ++ + A  L+   D DGD LL   
Sbjct: 8   SPCESSALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEA 67

Query: 107 DFERLVKGGEEEEKED-----LKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECM 166
           +F RLV+  E EE+++     L+ AF M+E E G G I P  L++M  RLG  + I++C 
Sbjct: 68  EFVRLVQAAEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDCR 127

Query: 167 AMIGVFDVDGDGVIDYREFLRMM 184
           AMI  FD++GDGV+ + EF  MM
Sbjct: 128 AMICRFDLNGDGVLSFDEFKIMM 149

[Close]

BLAST of Carg08742 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.1e-96
Identity = 179/184 (97.28%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPP LLLTPAPPLAEAAFRHLDA
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPP-LLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDELQSYFASVGEYMTRD+ARSLIREFDGDGDLLLELGDFERLVKGGE+EEKE
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEQEEKE 120

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLKRAFEMFE EKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDG+GVIDYREFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFL 180

Query: 181 RMMT 185
           RMMT
Sbjct: 181 RMMT 183

[Close]

BLAST of Carg08742 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 7.0e-96
Identity = 177/184 (96.20%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MALSPISTPFKWFSNTN KLN PRLRFKHKPT SNPPPPPLLLLTPAPPLAEAAFRHLDA
Sbjct: 15  MALSPISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDA 74

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDELQSYFASVGEYMTRD+ARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE
Sbjct: 75  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 134

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLK AFEMFE EKGCGFIGP GLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL
Sbjct: 135 DLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 194

Query: 181 RMMT 185
           RMMT
Sbjct: 195 RMMT 198

[Close]

BLAST of Carg08742 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.8e-70
Identity = 138/184 (75.00%), Postives = 149/184 (80.98%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MA+S I+ PFKWF+ TN KLN P L  KHKP  S PP P        PPLAE AFRHLD 
Sbjct: 1   MAISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTP--------PPLAEVAFRHLDI 60

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDEL+SYFAS+GEYMT D A+S+I +FD DGD LLELGDFERLVKG EEE++E
Sbjct: 61  DGDGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEE 120

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLKRAFEMFE EKGCGFIG  GLQKMFGRLGYVKS E+CM MI VFDVDGDGVIDY EFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFL 176

Query: 181 RMMT 185
           RMMT
Sbjct: 181 RMMT 176

[Close]

BLAST of Carg08742 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.8e-70
Identity = 138/184 (75.00%), Postives = 149/184 (80.98%), Query Frame = 0


Query: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60
           MA+S I+ PFKWF+ TN KLN P L  KHKP  S PP P        PPLAE AFRHLD 
Sbjct: 1   MAISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTP--------PPLAEVAFRHLDI 60

Query: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKE 120
           DGDGKISGDEL+SYFAS+GEYMT D A+S+I +FD DGD LLELGDFERLVKG EEE++E
Sbjct: 61  DGDGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEE 120

Query: 121 DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFL 180
           DLKRAFEMFE EKGCGFIG  GLQKMFGRLGYVKS E+CM MI VFDVDGDGVIDY EFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFL 176

Query: 181 RMMT 185
           RMMT
Sbjct: 181 RMMT 176

[Close]

BLAST of Carg08742 vs. ExPASy TrEMBL
Match: A0A6J1CDM6 (probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC111010501 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.2e-66
Identity = 132/178 (74.16%), Postives = 147/178 (82.58%), Query Frame = 0


Query: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 65
           +S  FKWFS +N KLN PRL F  KPT SNPPPPP   L  APPLAE AF HLDADGDGK
Sbjct: 8   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPP---LKAAPPLAEVAFHHLDADGDGK 67

Query: 66  ISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKRA 125
           IS DEL+SYFAS+GE +TRD+ R +IR+FDG+GD LLEL DF RL++  EEEE EDL+RA
Sbjct: 68  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 127

Query: 126 FEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 184
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM
Sbjct: 128 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 182

[Close]

BLAST of Carg08742 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 131.3 bits (329), Expect = 7.7e-31
Identity = 84/197 (42.64%), Postives = 105/197 (53.30%), Query Frame = 0


Query: 5   PISTPFKWFSNTNLKLNLPRLRFKHKP----------TRSNPPPPPLLLLTPA-PPLAEA 64
           P S  FKWFS   LKLNL     +  P           RSN      +    A       
Sbjct: 8   PSSNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQ 67

Query: 65  AFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVK- 124
            F H D+DGDGKIS  EL+ YF SVGEY++ + A+  I E D D D  L   DF  L+  
Sbjct: 68  VFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTR 127

Query: 125 -----GGEEEEKEDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFD 184
                 GE +   +LK AFEMFE EKG G I P GLQKM  +LG  ++  EC AMI  +D
Sbjct: 128 RDLYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYD 187

[Close]

BLAST of Carg08742 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 99.4 bits (246), Expect = 3.3e-21
Identity = 53/133 (39.85%), Postives = 85/133 (63.91%), Query Frame = 0


Query: 52  EAAFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLV 111
           EA F ++DA+ DG+IS +ELQ  F ++GE ++ + A + +R  D DGD +L+  +F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 112 KGGEEEEKE-DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDG 171
           K  +EEEK+ +LK AF ++  E G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 172 DGVIDYREFLRMM 184
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Carg08742 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 99.4 bits (246), Expect = 3.3e-21
Identity = 53/133 (39.85%), Postives = 85/133 (63.91%), Query Frame = 0


Query: 52  EAAFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLV 111
           EA F ++DA+ DG+IS +ELQ  F ++GE ++ + A + +R  D DGD +L+  +F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 112 KGGEEEEKE-DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDG 171
           K  +EEEK+ +LK AF ++  E G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 172 DGVIDYREFLRMM 184
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Carg08742 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 96.7 bits (239), Expect = 2.1e-20
Identity = 51/136 (37.50%), Postives = 83/136 (61.03%), Query Frame = 0


Query: 52  EAAFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLV 111
           EA F ++DA+ DG+IS +EL+  F ++GE M+ + A + ++  D DGD +L++ +F  L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 112 KGG----EEEEKEDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFD 171
           KG     EEE+K  +  AF M+  + G   I P  L+ M  +LG  ++ ++C  MI  FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIAD-GEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 172 VDGDGVIDYREFLRMM 184
           ++ DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

[Close]

BLAST of Carg08742 vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 89.4 bits (220), Expect = 3.4e-18
Identity = 51/133 (38.35%), Postives = 79/133 (59.40%), Query Frame = 0


Query: 52  EAAFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLV 111
           EA F ++DA+ DG+IS +ELQ  F ++GE +  D           DGD +L+  +F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLLSDT----------DGDGMLDFEEFSQLI 104

Query: 112 KGGEEEEKE-DLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDG 171
           K  +EEEK+ +LK AF ++  E G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 172 DGVIDYREFLRMM 184
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 166

The following BLAST results are available for this feature:

BLAST of Carg08742 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024217.1	5.6e-100	100.00	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. a...	[more]
KAG6591338.1	1.4e-98	98.91	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023535419.1	4.9e-96	96.20	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022936314.1	8.4e-96	97.28	probable calcium-binding protein CML41 [Cucurbita moschata]	[more]
XP_022974919.1	1.4e-95	96.20	probable calcium-binding protein CML41 [Cucurbita maxima]	[more]

BLAST of Carg08742 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	1.1e-29	42.64	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q9SRE6	4.6e-20	39.85	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q9SRE7	3.0e-19	37.50	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]
Q8RYJ8	8.7e-19	44.78	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYK0	1.5e-18	41.26	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Carg08742 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F7Y7	4.1e-96	97.28	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1IIY2	7.0e-96	96.20	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A5A7UHX6	3.8e-70	75.00	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	3.8e-70	75.00	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]
A0A6J1CDM6	1.2e-66	74.16	probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Carg08742 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	7.7e-31	42.64	calmodulin-like 41	[more]
AT1G76650.1	3.3e-21	39.85	calmodulin-like 38	[more]
AT1G76650.3	3.3e-21	39.85	calmodulin-like 38	[more]
AT1G76640.1	2.1e-20	37.50	Calcium-binding EF-hand family protein	[more]
AT1G76650.2	3.4e-18	38.35	calmodulin-like 38	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 112..132
None	No IPR available	GENE3D	1.10.238.10		coord: 44..113 e-value: 5.5E-14 score: 54.5
None	No IPR available	GENE3D	1.10.238.10		coord: 114..184 e-value: 6.8E-16 score: 60.1
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 17..184
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 17..184
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 86..114 e-value: 31.0 score: 5.7 coord: 158..184 e-value: 0.36 score: 19.9 coord: 50..78 e-value: 7.2E-4 score: 28.9
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 158..184 e-value: 5.0E-7 score: 28.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 53..111 e-value: 8.6E-10 score: 38.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 46..81 score: 12.951263
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 154..184 score: 13.899706
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 82..117 score: 8.23694
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 52..99 e-value: 9.46482E-11 score: 53.3205
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 121..184 e-value: 3.93456E-10 score: 51.3945
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 59..71
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 167..179
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 53..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08742-RA	Carg08742-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity