Carg08722 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08722
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	maf-like protein DDB_G0281937 isoform X1
Location	Carg_Chr09: 668055 .. 670729 (-)
RNA-Seq Expression	Carg08722
Synteny	Carg08722

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTACCTTTTCTCTCTCTCTCAAAATGAAGTCTGCAGATATTGATGAGAAAGCCATACGCAAAGAAAAGCCGGAAGAGTTGGTTGTGGCTCTTGCAGAGGCAAAGGTTAGGTATCATCTTGTGATTCATTTGTGCTCCACCATATTCTGCAATTGAAATATTAATGCGTATGTTATTGGTTCCCTTTTGTAAATTGCTTGTGCTGTATGAGCACTGTCGCCAAAAGATAATCTTGGATTTGTGATTGTCTTTGGTTCCATGCTATCATGACAGGCTAGGATTTAGAAGCTTCTATTAGCTACACAATATTTCTAAAACCATCCTAGACTGGATACAGTGATTTGCATAGTTTGGCATGTGATTATTTTGTGCACGCATCTTGTATTAATTAGGTATGAGTTTCATCTCAGGCTGACGCCATCATATCCAACTTACAAAAGAATCATACACATGAAGAGGAAGCAGATTCCACAATTTTGATTGCTGCTGACACAGTATGTTTTCCTATGTTAGTGTTAGTCGTTATTCTCTCTGTTAAACAATATGATGCATGGTGGCTTGGAGTTAATCTTTTCCAGTGTGCAATTTTTCGTTGCTCTTTGAAGTCTTAGCTGAATTGACTGTTTCATGTCAAAGGTTCTTCCACTATCTTTCACAATTTTAGTACGGGGTTTGGTTGTGGTAGTAGGTGGCTTTGGCTTATTTCTTAGATGATGGATATTTATTGTCAGGCAGAAGCCATCTCAGGAACACTCTCCATCAATGATTTTACGGACACACAGACATATAGATAATTTTTTATTAAATCAAGTCCTTTGACATCCGTCTGTTTTTATATATTTTTTATATGTTTTCTTAATTTTCAACTTGATCCATATAGATAATTTTTTTGTGCGATGTCCATTGCACGCCTTTATCTACTTGACTCAGTAAAGATAATAAGAGTTTTGTTGCCTTCTAAATTGTCATGTTGAATTGTCTTCGTATTTTCAAAATTGACATCAGAACTGTAGACATTTCTAATGTTTTTATTATTTGCATCTCTAAAACTTTTTTTTAAAGATAATAATAAAAAGTTGATTAGTATGATTTTCATATCTGACATTCTTTCTTTCTTTCTTCAATCATGCAGCCAATAATGGCAAGTTGATGCTTAATGGTCTTTCCTCAGTCTGTTCCCTTAACCAATAACCAGATTAAATATATGGCCAATCCCATGTGAAAGACCTCCCGCCTATATTGTGCCCCTGTTGTACTCATTCACCTTGAGATAGAAGTTTATGGAACAGTTCTCTTGTTATATATGTATTCTTATACCCGTTGCATATTGTATCCTCAAAACAGAAATGGTCTTATCGTGATAACTTGTGCCATTACGACCCTTTCTTGTTCTGTTTGCAAAGTTCCTCTTAACATACTTTCTTACTTCTATTGGTAATTGATGTGGAGGTGGTGATCTATGAAGGTGTCATCAGAGAAAAACCAGCCGGCAAGGAGGAAGCACGACAATTTATGAAAGGTTTGTTCACATAATACTAATAGTACTGCATTTCTATGTTTTTACTATTTCTTGAATGTTTTGGTTCGATTAAAAATTATTTTGCAGATTATTCAGGAGGGCATGCAGCGACACTTGGATCTGTATTTGTTAGGAATCTTAAAACAGGGTTTAGGAAAGGAGAATGGGATCGTGTAGAGGTATAAATATACATGTTCTTTTATTGATGATAGTATTTTAATGAAAAGACACTCGCGTTATGGAGACTGTTACCTTCTAATTGATTCAACAAAACAAATGGAATGATATCCTACTTTCATAGACCTAAACTTCTTCAATCAAACAACTTGTCTTGGTATGTGCTCTGCTGTGGGACCAGCCAAATAGATGCTAGATACTGTTATTTTGCATCGAGGGAAGGAGCTTCCTGTTCTGCTTATCCAATATTGATTTTCTAATTCCTAGCAACTGTTATTTTGCATCGAGGGAATGAGCTTCCTGTTCTGTTTTACAATATCTCATGTTTTTTTGTTTTCTTATGCTATCCGATATTGACGAGTGCTCTTACAGATCTTCTTCCACGAAATACCTGATGAAGTCATCGACAAGCTGGTAGAGATTTCACTTTCACTGGTTGTTGGTTGTGTTTTCTCTAGGAAAAGGAAATGTTGTATCTTTTGTCATCTTGTTTTTCCTTTTTTCTATACATTTCAGGTCGAGGAGGGGAATGTGCTCAATGTTGCCGGAGGGCTGATTATCGAGCATCCTTTGATTTTACCCTATGTGAAAAAAGTGGTTAGCTTCCCTTCTTCCTTTCCTTTTCCCATCATTTTCCTTTTCCTTCCCTTTTCCATTTTACTTTTTAAACATGAATTTTAGTTTTTAAATTTTGTTTTTTCAACATAAGTTTTTTTTTTTATCACATGGGTAAACGTGTGTTGCACGCAAGTTTCCTCGGTCTATAAATCCTGAAGATTAAGACTCTTTTTAACTGAGGAGATAGAGTTTTACATATGGCCAACAAATCGGTTGGGACAATCCACAAATATGCCTATCGTTCTTGATTTCTTGTAGATACCTTACGGGCTTTGGATCTCTGCAGGTCGGGACGACAGATAGTGTGATGGGCCTTCCCAAGGCTCTGACTGAGAAACTCTTGAAGGAAGCCATTTAG

mRNA sequence

ATGTTTACCTTTTCTCTCTCTCTCAAAATGAAGTCTGCAGATATTGATGAGAAAGCCATACGCAAAGAAAAGCCGGAAGAGTTGGTTGTGGCTCTTGCAGAGGCAAAGGCTGACGCCATCATATCCAACTTACAAAAGAATCATACACATGAAGAGGAAGCAGATTCCACAATTTTGATTGCTGCTGACACAGTGGTGATCTATGAAGGTGTCATCAGAGAAAAACCAGCCGGCAAGGAGGAAGCACGACAATTTATGAAAGATTATTCAGGAGGGCATGCAGCGACACTTGGATCTGTATTTGTTAGGAATCTTAAAACAGGGTTTAGGAAAGGAGAATGGGATCGTGTAGAGATCTTCTTCCACGAAATACCTGATGAAGTCATCGACAAGCTGGTAGAGATTTCACTTTCACTGGTTGTTGGTTGTGTTTTCTCTAGGAAAAGGAAATGTTGTATCTTTTGTCATCTTGTTTTTCCTTTTTTCTATACATTTCAGGTCGAGGAGGGGAATGTGCTCAATGTTGCCGGAGGGCTGATTATCGAGCATCCTTTGATTTTACCCTATGTGAAAAAAGTGGTCGGGACGACAGATAGTGTGATGGGCCTTCCCAAGGCTCTGACTGAGAAACTCTTGAAGGAAGCCATTTAG

Coding sequence (CDS)

ATGTTTACCTTTTCTCTCTCTCTCAAAATGAAGTCTGCAGATATTGATGAGAAAGCCATACGCAAAGAAAAGCCGGAAGAGTTGGTTGTGGCTCTTGCAGAGGCAAAGGCTGACGCCATCATATCCAACTTACAAAAGAATCATACACATGAAGAGGAAGCAGATTCCACAATTTTGATTGCTGCTGACACAGTGGTGATCTATGAAGGTGTCATCAGAGAAAAACCAGCCGGCAAGGAGGAAGCACGACAATTTATGAAAGATTATTCAGGAGGGCATGCAGCGACACTTGGATCTGTATTTGTTAGGAATCTTAAAACAGGGTTTAGGAAAGGAGAATGGGATCGTGTAGAGATCTTCTTCCACGAAATACCTGATGAAGTCATCGACAAGCTGGTAGAGATTTCACTTTCACTGGTTGTTGGTTGTGTTTTCTCTAGGAAAAGGAAATGTTGTATCTTTTGTCATCTTGTTTTTCCTTTTTTCTATACATTTCAGGTCGAGGAGGGGAATGTGCTCAATGTTGCCGGAGGGCTGATTATCGAGCATCCTTTGATTTTACCCTATGTGAAAAAAGTGGTCGGGACGACAGATAGTGTGATGGGCCTTCCCAAGGCTCTGACTGAGAAACTCTTGAAGGAAGCCATTTAG

Protein sequence

MFTFSLSLKMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVKKVVGTTDSVMGLPKALTEKLLKEAI

Homology

[Close]

BLAST of Carg08722 vs. NCBI nr
Match: KAG7024197.1 (Maf-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 424.1 bits (1089), Expect = 7.3e-115
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   MFTFSLSLKMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILI 60
           MFTFSLSLKMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILI
Sbjct: 1   MFTFSLSLKMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILI 60

Query: 61  AADTVVIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIF 120
           AADTVVIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIF
Sbjct: 61  AADTVVIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIF 120

Query: 121 FHEIPDEVIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLI 180
           FHEIPDEVIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLI
Sbjct: 121 FHEIPDEVIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLI 180

Query: 181 IEHPLILPYVKKVVGTTDSVMGLPKALTEKLLKEAI 217
           IEHPLILPYVKKVVGTTDSVMGLPKALTEKLLKEAI
Sbjct: 181 IEHPLILPYVKKVVGTTDSVMGLPKALTEKLLKEAI 216

[Close]

BLAST of Carg08722 vs. NCBI nr
Match: KAG6591314.1 (7-methyl-GTP pyrophosphatase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 313.5 bits (802), Expect = 1.4e-81
Identity = 169/205 (82.44%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKADA+ISNLQKNHTHEEEADSTILIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKADALISNLQKNHTHEEEADSTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPAGKEEARQFMKDYSGGHAATLGSVFV NLKTGFRKGEWDRVEIFFHEIPDEVIDK
Sbjct: 94  IREKPAGKEEARQFMKDYSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFHEIPDEVIDK 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEGNVLNVAGGLIIEHPLILPYVK
Sbjct: 154 L----------------------------------VEEGNVLNVAGGLIIEHPLILPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           KVVGTTDSVMGLPKALTEKLLKEAI
Sbjct: 214 KVVGTTDSVMGLPKALTEKLLKEAI 204

[Close]

BLAST of Carg08722 vs. NCBI nr
Match: XP_022936457.1 (maf-like protein DDB_G0281937 isoform X1 [Cucurbita moschata] >XP_022936459.1 maf-like protein DDB_G0281937 isoform X1 [Cucurbita moschata])

HSP 1 Score: 312.0 bits (798), Expect = 4.0e-81
Identity = 169/205 (82.44%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPAGKEEARQFMKDYSGGHAATLGSVFV NLKTGFRKGEWDRVEIFFHEIPDEVID 
Sbjct: 94  IREKPAGKEEARQFMKDYSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFHEIPDEVIDM 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEGNVLNVAGGLIIEHPLILPYVK
Sbjct: 154 L----------------------------------VEEGNVLNVAGGLIIEHPLILPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           KVVGTTDSVMGLPKALTEKLLKEAI
Sbjct: 214 KVVGTTDSVMGLPKALTEKLLKEAI 204

[Close]

BLAST of Carg08722 vs. NCBI nr
Match: XP_022976356.1 (maf-like protein DDB_G0281937 isoform X2 [Cucurbita maxima] >XP_022976357.1 maf-like protein DDB_G0281937 isoform X2 [Cucurbita maxima])

HSP 1 Score: 308.1 bits (788), Expect = 5.8e-80
Identity = 166/205 (80.98%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKA+AIISNLQKNHTHEEEAD TILIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKAEAIISNLQKNHTHEEEADPTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPAGKEEARQFMKDYSGGHAATLGSVFV NLKTGFRKGEWDRVEIFFHEIPDEVIDK
Sbjct: 94  IREKPAGKEEARQFMKDYSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFHEIPDEVIDK 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEGNVLNVAGGLIIEHP ILPYVK
Sbjct: 154 L----------------------------------VEEGNVLNVAGGLIIEHPFILPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           KVVGTTDSVMGLPKALTE+LLKEAI
Sbjct: 214 KVVGTTDSVMGLPKALTERLLKEAI 204

[Close]

BLAST of Carg08722 vs. NCBI nr
Match: XP_023536431.1 (maf-like protein DDB_G0281937 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.4 bits (781), Expect = 3.8e-79
Identity = 166/205 (80.98%), Postives = 167/205 (81.46%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKA+AIISNLQKNH  EEEAD TILIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKAEAIISNLQKNHRREEEADPTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPAGKEEARQFMKDYSGGHAATLGSVFV NLKTGFRKGEWDRVEIFFHEIPDEVIDK
Sbjct: 94  IREKPAGKEEARQFMKDYSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFHEIPDEVIDK 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEGNVLNVAGGLIIEHPLILPYVK
Sbjct: 154 L----------------------------------VEEGNVLNVAGGLIIEHPLILPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           KVVGTTDSVMGLPKALTEKLLKEAI
Sbjct: 214 KVVGTTDSVMGLPKALTEKLLKEAI 204

[Close]

BLAST of Carg08722 vs. ExPASy Swiss-Prot
Match: Q54TC5 (7-methyl-GTP pyrophosphatase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0281937 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 7.0e-20
Identity = 62/207 (29.95%), Postives = 109/207 (52.66%), Query Frame = 0


Query: 9   KMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEAD-STILIAADTVVI 68
           K  S DIDEKAIR   P+ L + ++ AKA A++  ++++   ++E D  +I+I +D V++
Sbjct: 27  KTMSPDIDEKAIRDSDPKTLTLLISRAKAQALLKRIKES---DDELDKKSIMICSDQVIV 86

Query: 69  YEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDE 128
           + GVIREKP  +++ R++++ Y    A  + SV V N++TG      D     F +I DE
Sbjct: 87  HNGVIREKPETEQQCREYLQSYEFHPAVAVVSVVVVNIETGKIVEGTDIATQHFKKISDE 146

Query: 129 VIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLIL 188
            IDKL                                  +++G+V++ AGG  +EH  + 
Sbjct: 147 FIDKL----------------------------------IKQGDVMHCAGGFTVEH--MA 194

Query: 189 PYVKKVVGTTDSVMGLPKALTEKLLKE 215
            +  ++ G  ++++GLPK LT+ L+ +
Sbjct: 207 DFTLQLEGEVETILGLPKTLTKNLISQ 194

[Close]

BLAST of Carg08722 vs. ExPASy Swiss-Prot
Match: Q5QZ35 (7-methyl-GTP pyrophosphatase OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) OX=283942 GN=IL1346 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.8e-07
Identity = 40/130 (30.77%), Postives = 66/130 (50.77%), Query Frame = 0


Query: 6   LSLKMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTV 65
           L+  +   DIDE AI  E P++LV  LAEAKA A+    +K  T+    D+ I+I +D V
Sbjct: 23  LNYDVVKPDIDESAISSESPQQLVGRLAEAKARAV----EKRMTY----DNAIIIGSDQV 82

Query: 66  VIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIP 125
            + +G I  KP  +E A + +  + G        + V N +    +   +  E+ F ++ 
Sbjct: 83  AVCDGNILGKPGNRENAVRQLSSFIGKTVTFYTGLAVFNTEAQQCEVRVEPFEVEFRQLT 142

Query: 126 DEVIDKLVEI 136
            E I++ VE+
Sbjct: 143 AEEIERYVEL 144

[Close]

BLAST of Carg08722 vs. ExPASy Swiss-Prot
Match: Q9PK45 (Nucleoside triphosphate pyrophosphatase OS=Chlamydia muridarum (strain MoPn / Nigg) OX=243161 GN=TC_0628 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-06
Identity = 55/205 (26.83%), Postives = 90/205 (43.90%), Query Frame = 0


Query: 12  SADIDEKAIR-KEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEG 71
           S+D DE++I     P E    LA  KA+A+ S         +    +++I ADTVV+YEG
Sbjct: 29  SSDFDERSITYSGDPFEYTRELAWNKANAVRS---------QGFSDSLIITADTVVVYEG 88

Query: 72  VIREKPAGKEEARQFMKDYSG-GHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVI 131
            +  KP  +E A + ++  SG  H+     V ++N K        +  ++ F +IP + +
Sbjct: 89  EVFNKPESEEHAVEMLRTLSGTSHSVITSLVLMQNEKVASAS---ETTQVSFIDIPPQHL 148

Query: 132 DKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPY 191
              V+          FS  ++C  +C           V++G      GGLII        
Sbjct: 149 KTYVQ---------AFSSLKRCGGYC-----------VQDG------GGLII-------- 186

Query: 192 VKKVVGTTDSVMGLPKALTEKLLKE 215
            K++ G   ++ GLP     +LL E
Sbjct: 209 -KQIEGCVYNIQGLPIKTLNQLLME 186

[Close]

BLAST of Carg08722 vs. ExPASy Swiss-Prot
Match: B4UJ23 (dTTP/UTP pyrophosphatase OS=Anaeromyxobacter sp. (strain K) OX=447217 GN=AnaeK_1353 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 5.8e-06
Identity = 57/213 (26.76%), Postives = 89/213 (41.78%), Query Frame = 0


Query: 6   LSLKMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTV 65
           L+L+++ AD DE+ +  E P + V+ +A  KA A+  +L              ++AADT 
Sbjct: 23  LALEIRPADTDERVLPGEPPRDYVLRVAREKARAVPGDL--------------VLAADTA 82

Query: 66  VIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRV---EIFFH 125
           V+  G +  KP   ++AR+ ++  SG     L  V VR    G    E D V   E+ F 
Sbjct: 83  VVLGGEVLGKPRDADDARRMLRALSGTRHEVLTGVCVRR-NAGALGVELDAVVATEVAFA 142

Query: 126 EIPDEVIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIE 185
            + D  ID                                  + V  G  L+ AG   I+
Sbjct: 143 RLGDGEID----------------------------------WYVGTGEPLDKAGAYAIQ 185

Query: 186 HPLILPYVKKVVGTTDSVMGLPKALTEKLLKEA 216
                 +V++V G+  +V+GLP A T  LL+ A
Sbjct: 203 GS-GGAFVEEVRGSVSNVVGLPLAETAALLRRA 185

[Close]

BLAST of Carg08722 vs. ExPASy Swiss-Prot
Match: B8JH92 (dTTP/UTP pyrophosphatase OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) OX=455488 GN=A2cp1_1450 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 7.5e-06
Identity = 56/213 (26.29%), Postives = 90/213 (42.25%), Query Frame = 0


Query: 6   LSLKMKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTV 65
           L+L+++ AD DE+ +  E P + V+ +A  KA A+  +L              ++AADT 
Sbjct: 23  LALEIRPADTDERVLPGEPPRDYVLRVAREKARAVPGDL--------------VLAADTA 82

Query: 66  VIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRV---EIFFH 125
           V+  G +  KP   ++AR+ ++  SG     L +V VR   +     E D V   E+ F 
Sbjct: 83  VVLGGEVLGKPRDADDARRMLRALSGTRHEVLTAVCVRRNASALGV-ELDAVVATEVAFA 142

Query: 126 EIPDEVIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIE 185
            + D  ID                                  + V  G  L+ AG   I+
Sbjct: 143 RLGDAEID----------------------------------WYVGTGEPLDKAGAYAIQ 185

Query: 186 HPLILPYVKKVVGTTDSVMGLPKALTEKLLKEA 216
                 +V++V G+  +V+GLP A T  LL+ A
Sbjct: 203 GS-GGAFVEEVRGSVSNVVGLPLAETAALLRRA 185

[Close]

BLAST of Carg08722 vs. ExPASy TrEMBL
Match: A0A6J1F8C6 (maf-like protein DDB_G0281937 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443072 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.0e-81
Identity = 169/205 (82.44%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPAGKEEARQFMKDYSGGHAATLGSVFV NLKTGFRKGEWDRVEIFFHEIPDEVID 
Sbjct: 94  IREKPAGKEEARQFMKDYSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFHEIPDEVIDM 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEGNVLNVAGGLIIEHPLILPYVK
Sbjct: 154 L----------------------------------VEEGNVLNVAGGLIIEHPLILPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           KVVGTTDSVMGLPKALTEKLLKEAI
Sbjct: 214 KVVGTTDSVMGLPKALTEKLLKEAI 204

[Close]

BLAST of Carg08722 vs. ExPASy TrEMBL
Match: A0A6J1IGP5 (maf-like protein DDB_G0281937 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476783 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.8e-80
Identity = 166/205 (80.98%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKA+AIISNLQKNHTHEEEAD TILIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKAEAIISNLQKNHTHEEEADPTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPAGKEEARQFMKDYSGGHAATLGSVFV NLKTGFRKGEWDRVEIFFHEIPDEVIDK
Sbjct: 94  IREKPAGKEEARQFMKDYSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFHEIPDEVIDK 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEGNVLNVAGGLIIEHP ILPYVK
Sbjct: 154 L----------------------------------VEEGNVLNVAGGLIIEHPFILPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           KVVGTTDSVMGLPKALTE+LLKEAI
Sbjct: 214 KVVGTTDSVMGLPKALTERLLKEAI 204

[Close]

BLAST of Carg08722 vs. ExPASy TrEMBL
Match: A0A6J1IJ84 (maf-like protein DDB_G0281937 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476783 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.9e-78
Identity = 166/214 (77.57%), Postives = 168/214 (78.50%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKA+AIISNLQKNHTHEEEAD TILIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKAEAIISNLQKNHTHEEEADPTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPAGKEEARQFMKDYSGGHAATLGSVFV NLKTGFRKGEWDRVEIFFHEIPDEVIDK
Sbjct: 94  IREKPAGKEEARQFMKDYSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFHEIPDEVIDK 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEGNVLNVAGGLIIEHP ILPYVK
Sbjct: 154 L----------------------------------VEEGNVLNVAGGLIIEHPFILPYVK 213

Query: 192 KV---------VGTTDSVMGLPKALTEKLLKEAI 217
           KV         VGTTDSVMGLPKALTE+LLKEAI
Sbjct: 214 KVILTGFGSLQVGTTDSVMGLPKALTERLLKEAI 213

[Close]

BLAST of Carg08722 vs. ExPASy TrEMBL
Match: A0A6J1BUA5 (maf-like protein DDB_G0281937 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005415 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.1e-74
Identity = 156/205 (76.10%), Postives = 163/205 (79.51%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           +ADIDEKAIRKEKPEELVVALAEAKADAIISNL K HTHE+EA+ TILIAADTVVIYEGV
Sbjct: 34  TADIDEKAIRKEKPEELVVALAEAKADAIISNLHKIHTHEKEAEPTILIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKP  KEEARQFMKDYSGGHAATLGSV V NLKTGFRKGEWD+VEI+FHEIPDEVIDK
Sbjct: 94  IREKPDSKEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDQVEIYFHEIPDEVIDK 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEG VLNVAGGLIIEHPLI+PYVK
Sbjct: 154 L----------------------------------VEEGIVLNVAGGLIIEHPLIIPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           +VVGTTDSVMGLPKALTEKLLKEAI
Sbjct: 214 EVVGTTDSVMGLPKALTEKLLKEAI 204

[Close]

BLAST of Carg08722 vs. ExPASy TrEMBL
Match: A0A1S3CSV1 (maf-like protein DDB_G0281937 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503944 PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 5.7e-73
Identity = 155/205 (75.61%), Postives = 162/205 (79.02%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           SADIDEKAIRKEKPEELVVALAEAKADAIISNLQ   THE+EA+ T+LIAADTVVIYEGV
Sbjct: 34  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQNIDTHEKEAEQTVLIAADTVVIYEGV 93

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKPA KEEARQF+KDYSGGHAATLGSV V NLKTGFRKGEWDRVEIFF+EIPDEVIDK
Sbjct: 94  IREKPASKEEARQFLKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIFFNEIPDEVIDK 153

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L                                  VEEG VL VAGGLIIEHPLILPYVK
Sbjct: 154 L----------------------------------VEEGTVLYVAGGLIIEHPLILPYVK 204

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
           +VVGTTDSVMGLPKALTEKLLKEA+
Sbjct: 214 EVVGTTDSVMGLPKALTEKLLKEAM 204

[Close]

BLAST of Carg08722 vs. TAIR 10
Match: AT5G42770.1 (Maf-like protein )

HSP 1 Score: 204.1 bits (518), Expect = 1.1e-52
Identity = 114/207 (55.07%), Postives = 139/207 (67.15%), Query Frame = 0


Query: 10  MKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYE 69
           + SADIDEK+IRKEKPEELV+ALAEAKA+AI+  +      EE+  ST+LI  D VV+YE
Sbjct: 32  LMSADIDEKSIRKEKPEELVLALAEAKAEAIMQRIPDGENIEED-KSTLLITCDQVVVYE 91

Query: 70  GVIREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVI 129
             +REKP+  EEAR++++ YS GH AT+ SV V NLKTG RKG  DRVEI+F+EIP+E I
Sbjct: 92  DAVREKPSSVEEAREYIRGYSKGHTATVSSVAVTNLKTGVRKGGVDRVEIYFNEIPEETI 151

Query: 130 DKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPY 189
           +KL                                  +EEG VL VAG L+IEHPLILP 
Sbjct: 152 EKL----------------------------------IEEGMVLKVAGALLIEHPLILPC 203

Query: 190 VKKVVGTTDSVMGLPKALTEKLLKEAI 217
           VK+VVGTTDSVMGLPK LTEKL+KE +
Sbjct: 212 VKEVVGTTDSVMGLPKELTEKLIKEVL 203

[Close]

BLAST of Carg08722 vs. TAIR 10
Match: AT5G42770.2 (Maf-like protein )

HSP 1 Score: 201.4 bits (511), Expect = 7.1e-52
Identity = 118/233 (50.64%), Postives = 142/233 (60.94%), Query Frame = 0


Query: 10  MKSADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEA--------------- 69
           + SADIDEK+IRKEKPEELV+ALAEAKADAI+S LQ +   +EE                
Sbjct: 32  LMSADIDEKSIRKEKPEELVLALAEAKADAIVSKLQISECEDEEQPRVLIASDTAEAIMQ 91

Query: 70  -----------DSTILIAADTVVIYEGVIREKPAGKEEARQFMKDYSGGHAATLGSVFVR 129
                       ST+LI  D VV+YE  +REKP+  EEAR++++ YS GH AT+ SV V 
Sbjct: 92  RIPDGENIEEDKSTLLITCDQVVVYEDAVREKPSSVEEAREYIRGYSKGHTATVSSVAVT 151

Query: 130 NLKTGFRKGEWDRVEIFFHEIPDEVIDKLVEISLSLVVGCVFSRKRKCCIFCHLVFPFFY 189
           NLKTG RKG  DRVEI+F+EIP+E I+KL                               
Sbjct: 152 NLKTGVRKGGVDRVEIYFNEIPEETIEKL------------------------------- 211

Query: 190 TFQVEEGNVLNVAGGLIIEHPLILPYVKKVVGTTDSVMGLPKALTEKLLKEAI 217
              +EEG VL VAG L+IEHPLILP VK+VVGTTDSVMGLPK LTEKL+KE +
Sbjct: 212 ---IEEGMVLKVAGALLIEHPLILPCVKEVVGTTDSVMGLPKELTEKLIKEVL 230

[Close]

BLAST of Carg08722 vs. TAIR 10
Match: AT5G66550.1 (Maf-like protein )

HSP 1 Score: 195.3 bits (495), Expect = 5.1e-50
Identity = 103/205 (50.24%), Postives = 132/205 (64.39%), Query Frame = 0


Query: 12  SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
           +ADIDEKAIR EKPE+LVVALAEAKA+ IIS L       ++   T+LI ADTVV+Y+GV
Sbjct: 36  TADIDEKAIRTEKPEDLVVALAEAKANEIISKLGGESQFAKDPQPTLLITADTVVVYKGV 95

Query: 72  IREKPAGKEEARQFMKDYSGGHAATLGSVFVRNLKTGFRKGEWDRVEIFFHEIPDEVIDK 131
           IREKP  KEEAR+F+K YSG H   +GSV VRNLKTG +KG WD+ E++FHEIP++VID 
Sbjct: 96  IREKPTTKEEAREFIKGYSGSHGGVVGSVLVRNLKTGVKKGGWDKAEVYFHEIPEQVIDG 155

Query: 132 LVEISLSLVVGCVFSRKRKCCIFCHLVFPFFYTFQVEEGNVLNVAGGLIIEHPLILPYVK 191
           L++ +++                                    VAGGL +EHPLI P++ 
Sbjct: 156 LIDDAIT----------------------------------YKVAGGLTLEHPLISPFID 206

Query: 192 KVVGTTDSVMGLPKALTEKLLKEAI 217
            VVG  D+VMGLPK LTEK + + +
Sbjct: 216 SVVGGVDTVMGLPKELTEKFINDVL 206

[Close]

BLAST of Carg08722 vs. TAIR 10
Match: AT2G25500.1 (Inosine triphosphate pyrophosphatase family protein )

HSP 1 Score: 75.1 bits (183), Expect = 7.7e-14
Identity = 39/63 (61.90%), Postives = 50/63 (79.37%), Query Frame = 0


Query: 12 SADIDEKAIRKEKPEELVVALAEAKADAIISNLQKNHTHEEEADSTILIAADTVVIYEGV 71
          +ADIDEKAIRKEKPE+LVV +AEAKA+ II  L   +   +++  T+LI ADTVV+Y+GV
Sbjct: 36 TADIDEKAIRKEKPEDLVVTIAEAKANEIILKLGGENQFTQDSQPTLLITADTVVVYKGV 95

Query: 72 IRE 75
          IRE
Sbjct: 96 IRE 98

The following BLAST results are available for this feature:

BLAST of Carg08722 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024197.1	7.3e-115	100.00	Maf-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6591314.1	1.4e-81	82.44	7-methyl-GTP pyrophosphatase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022936457.1	4.0e-81	82.44	maf-like protein DDB_G0281937 isoform X1 [Cucurbita moschata] >XP_022936459.1 ma...	[more]
XP_022976356.1	5.8e-80	80.98	maf-like protein DDB_G0281937 isoform X2 [Cucurbita maxima] >XP_022976357.1 maf-...	[more]
XP_023536431.1	3.8e-79	80.98	maf-like protein DDB_G0281937 isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg08722 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54TC5	7.0e-20	29.95	7-methyl-GTP pyrophosphatase OS=Dictyostelium discoideum OX=44689 GN=DDB_G028193...	[more]
Q5QZ35	6.8e-07	30.77	7-methyl-GTP pyrophosphatase OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM...	[more]
Q9PK45	2.6e-06	26.83	Nucleoside triphosphate pyrophosphatase OS=Chlamydia muridarum (strain MoPn / Ni...	[more]
B4UJ23	5.8e-06	26.76	dTTP/UTP pyrophosphatase OS=Anaeromyxobacter sp. (strain K) OX=447217 GN=AnaeK_1...	[more]
B8JH92	7.5e-06	26.29	dTTP/UTP pyrophosphatase OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC B...	[more]

BLAST of Carg08722 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F8C6	2.0e-81	82.44	maf-like protein DDB_G0281937 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1IGP5	2.8e-80	80.98	maf-like protein DDB_G0281937 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1IJ84	5.9e-78	77.57	maf-like protein DDB_G0281937 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1BUA5	5.1e-74	76.10	maf-like protein DDB_G0281937 isoform X2 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A1S3CSV1	5.7e-73	75.61	maf-like protein DDB_G0281937 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503944...	[more]

BLAST of Carg08722 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42770.1	1.1e-52	55.07	Maf-like protein	[more]
AT5G42770.2	7.1e-52	50.64	Maf-like protein	[more]
AT5G66550.1	5.1e-50	50.24	Maf-like protein	[more]
AT2G25500.1	7.7e-14	61.90	Inosine triphosphate pyrophosphatase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029001	Inosine triphosphate pyrophosphatase-like	GENE3D	3.90.950.10		coord: 163..215 e-value: 1.8E-8 score: 35.9 coord: 3..140 e-value: 1.3E-32 score: 114.8
IPR029001	Inosine triphosphate pyrophosphatase-like	SUPERFAMILY	52972	ITPase-like	coord: 153..214
IPR029001	Inosine triphosphate pyrophosphatase-like	SUPERFAMILY	52972	ITPase-like	coord: 7..135
IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein	PIRSF	PIRSF006305	Maf	coord: 164..216 e-value: 4.3E-5 score: 20.8 coord: 4..154 e-value: 8.8E-20 score: 68.8
IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein	PFAM	PF02545	Maf	coord: 7..134 e-value: 1.0E-24 score: 87.3 coord: 167..214 e-value: 9.8E-9 score: 35.2
IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein	PANTHER	PTHR43213	BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED	coord: 10..142 coord: 166..215
None	No IPR available	PANTHER	PTHR43213:SF12	MAF-LIKE PROTEIN	coord: 10..142
None	No IPR available	PANTHER	PTHR43213:SF12	MAF-LIKE PROTEIN	coord: 166..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08722-RA	Carg08722-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0047429	nucleoside-triphosphate diphosphatase activity