Carg08662 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08662
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSubtilisin-like protease
LocationCarg_Chr09: 398345 .. 401449 (+)
RNA-Seq ExpressionCarg08662
SyntenyCarg08662
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAACCATTTCTTCTTGTGTCTTCCTTTTGGTTTGTGTCATCTTCTTGCTTCTTTCTGAAAATGCTAAAATAGCTGCTGCTGCAGCTGGGAACAATGGCGTTTACATCGTCTACATGGGATCCGCGTCCAGGACTGATTTCCTTCGCCTTCTTACCTCTGTCAACAGGCGGTAAAGCTTAATAAATATCCCAATTTCTTGAAAAAGCAAATTTAAAATCTCACACATATTATACCCTGTTTCTTGTGTGGTACGTAGGAATGGAAACGCAGTGGTACGGACTTATAAACATGGGTTCACAGGATTCGCAGCTCGTCTGTCAGAGCAGGAAGCCCATGCCATGAGACAAAGGCCTGGAGTTGTTTCGGTCTTCCCAGATCCAATACTGAAGCTCCACACAACTCATTCATGGGATTTCTTGGTCAGTCAGACAAGCGTCAAAATCGACAGCGCTCCCAAGGCAGATCCCCCGACCTCTTCCTCTCAGCCGTCCGATACCATTATCGGCATCTTGGACACCGGTAACTAAATATGTAACAATATATCAACCATTAATTCTCTGCACTGCACCGTATAACAAGTTGCCGCGGTTCTAACAACTCGCCGGAAAATTTAGGTATCTGGCCGGAATCCGAGAGTTTTAGAGACGAGGGTATGCCTCCGATACCGTCGAGGTGGAAAGGTACGTGCATGGCTGGCGATGACTTCACTTCCTCCAACTGTAACAGGTAAGTGTTGCTAAAAATCCATAGCTATTTAAAAGTTTCGATAATAACTTGAAATTAAAAAAAAAAAAAAAAAACAATTTAAAGATGGAAGCTGTTAGTGAATAAAAAATCATGGAACTATCGTGTGTGTGTGTTTGACATGTTTTGTCGGGTCAGAAAAATCGTTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGGATAAGGTTCCACTCACCTCGAGACGGAGCTGGACATGGAACCCACGTGGCTTCAACAGCGGCCGGCAGTGCCGTGGCTAATGCATCCTACTATGGCCTCGCAGCCGGGACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTATAGAGTCTGTATGGCCGATGGTTGTCATGGATCGTCAATCCTGGCAGCGTTTGATGATGCCATTAACGATGGGGTCGATGTATTATCCTTATCTCTCGGTGCGCCCACCTACTACAAACCGGAATTGACGACCGACCCCATTGCCATCGGAGCCTTTCATGCAGTTGAGAAGGGGATTACAGTTGTCTGCTCTGCTGGAAACGACGGCCCCACTCCGGGATCGGTGGTGAACGACGCGCCGTGGATTCTAACAGTGGCTGCCTCCACCATTGATAGGGATTTTGAGTCTGATGTTGTGCTGGGCAACAAAAAAGTGATCAAGGTAATTTAAATTCCTTCACCTCGTTAAGTTATCTCAAATAAACTGACCCAATGAACTATAATTCAGGGAGAAGGTATAAATTTCTCTGCTCTTGAAAGCTCTCCTGTATACCCACTGATACATGGCAAATCGGCCAACAAATCGAGTACCAGTGAAGACAGTGCGAGGTATGTAAGAGATGAGACATTATATGATGCTGCTGCTGGCTAATAAGATTCCTCATTAAGCTCTCTGATGAGTCCTCTGGCTGTTGTGTCTCTGTTTAGGATTTGTTCTGAAGGCTCCATGGACGAAGCTCTTGTGAAGGGGAAGATAGTTATTTGTGAGAGCAGTGAGGAAGGAGGCGGTTCCTATTGGCAAGGTCAGGCTGAGACAGTGGAGAGCCTTGGAGGGGTCGGGATAGTTCTCATTGATGACCAAACAAAGCTAGTGGCAGAGAAATTTACTTCTCCCATCACCGCTATTAGCTCAAAGGATGGCGATGAGGTCCTTGCCTATGTTCACTCAAGCAGGTAAAAGTTCAAACCATCCTCTGTTTAATCATCCTGGGTAGAAAACTTGAACTTCGTGGGTCTTCAATGGCCTAACCGAGTTTGATATTTTCTGAAGGAACCCAGTTGCTACAATCCTGCCCACCCTGACCGTAATAAACTATAAGCCAGCGCCCGCTATGGCATACTTTTCATCCAGAGGGCCCAATCCCGCAGTATTAAACATGATCAAGGTAAGGCAGCCTTCAAAGGGAAGAAGGAAGGATGAGGGGAAAGATTATATATTTGCCCTTCATCAACAAAATTTCAATCTAAATTTTAGATTTAACTGTGATGCAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCCTGGCTTGGAAATGACACGAACTCTACTCCGCAACCAGAAAATTCGCCGCTCTTCAATGTGATCTCAGGAACTTCAATGTCCTGCCCGCATGTCTCCGGCGTAGTAGCCATGGTAAAATCTCAAAACCCCACATGGAGTCCCTCAGCAATCAAGTCGGCCATCATGACAACAGGCAAGGAATTGACCTCACTAAATTTTTTGTTGAATTGAACATGTTTACTGATGAGCAATTTTCTCACGAAAACTTCACAGCAATCCAAACGAATAACTTGGAATCGCCGATGACTTTGGATACGGGATCAGTAGCTACACCTTATGATTATGGGGCAGGAGAGATATCAACCACCGGAGCATTAAAACCAGGACTGGTCTATGAAACTAGTACAACTGACTACTTAATCTACCTTTGTGGCCGAGGCTATAACCGGTCCACCATAAAGAGCATCGCAAATACTGTTCCTGATGGGTTTGATTGCCCCAAGGATTCTAATGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTGTCCGAATTGAAAGGCAGCGAAAGCAAGAAAATAGTGAGAACAGTTACAAATGTTGGTGGTGATGGTGCAACAGTTTACAAAGTCAGTGTGGACGCACCTGGGGAAGTAGATGTCAAAGTGATTCCAGAGAAATTGGAATTTTCAAAGAACAATCAGAAGCAGAGTTACAAAGTGGTGTTCACCTCAACTGTGTCCACACTGAAGAAAGAAGCCTTTGGTTCAATAACTTGGAGCAATGGAAAACATCGAGTCCGGAGTCCATTTGTGGTAACCAGTGAGAGTAGTAAGTCAGAAGATTAG

mRNA sequence

ATGCAAACCATTTCTTCTTGTGTCTTCCTTTTGGTTTGTGTCATCTTCTTGCTTCTTTCTGAAAATGCTAAAATAGCTGCTGCTGCAGCTGGGAACAATGGCGTTTACATCGTCTACATGGGATCCGCGTCCAGGACTGATTTCCTTCGCCTTCTTACCTCTGTCAACAGGCGGAATGGAAACGCAGTGGTACGGACTTATAAACATGGGTTCACAGGATTCGCAGCTCGTCTGTCAGAGCAGGAAGCCCATGCCATGAGACAAAGGCCTGGAGTTGTTTCGGTCTTCCCAGATCCAATACTGAAGCTCCACACAACTCATTCATGGGATTTCTTGGTCAGTCAGACAAGCGTCAAAATCGACAGCGCTCCCAAGGCAGATCCCCCGACCTCTTCCTCTCAGCCGTCCGATACCATTATCGGCATCTTGGACACCGGTATCTGGCCGGAATCCGAGAGTTTTAGAGACGAGGGTATGCCTCCGATACCGTCGAGGTGGAAAGGTACGTGCATGGCTGGCGATGACTTCACTTCCTCCAACTGTAACAGAAAAATCGTTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGGATAAGGTTCCACTCACCTCGAGACGGAGCTGGACATGGAACCCACGTGGCTTCAACAGCGGCCGGCAGTGCCGTGGCTAATGCATCCTACTATGGCCTCGCAGCCGGGACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTATAGAGTCTGTATGGCCGATGGTTGTCATGGATCGTCAATCCTGGCAGCGTTTGATGATGCCATTAACGATGGGGTCGATGTATTATCCTTATCTCTCGGTGCGCCCACCTACTACAAACCGGAATTGACGACCGACCCCATTGCCATCGGAGCCTTTCATGCAGTTGAGAAGGGGATTACAGTTGTCTGCTCTGCTGGAAACGACGGCCCCACTCCGGGATCGGTGGTGAACGACGCGCCGTGGATTCTAACAGTGGCTGCCTCCACCATTGATAGGGATTTTGAGTCTGATGTTGTGCTGGGCAACAAAAAAGTGATCAAGGGAGAAGGTATAAATTTCTCTGCTCTTGAAAGCTCTCCTGTATACCCACTGATACATGGCAAATCGGCCAACAAATCGAGTACCAGTGAAGACAGTGCGAGGATTTGTTCTGAAGGCTCCATGGACGAAGCTCTTGTGAAGGGGAAGATAGTTATTTGTGAGAGCAGTGAGGAAGGAGGCGGTTCCTATTGGCAAGGTCAGGCTGAGACAGTGGAGAGCCTTGGAGGGGTCGGGATAGTTCTCATTGATGACCAAACAAAGCTAGTGGCAGAGAAATTTACTTCTCCCATCACCGCTATTAGCTCAAAGGATGGCGATGAGGTCCTTGCCTATGTTCACTCAAGCAGGAACCCAGTTGCTACAATCCTGCCCACCCTGACCGTAATAAACTATAAGCCAGCGCCCGCTATGGCATACTTTTCATCCAGAGGGCCCAATCCCGCAGTATTAAACATGATCAAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCCTGGCTTGGAAATGACACGAACTCTACTCCGCAACCAGAAAATTCGCCGCTCTTCAATGTGATCTCAGGAACTTCAATGTCCTGCCCGCATGTCTCCGGCGTAGTAGCCATGCAATTTTCTCACGAAAACTTCACAGCAATCCAAACGAATAACTTGGAATCGCCGATGACTTTGGATACGGGATCAGTAGCTACACCTTATGATTATGGGGCAGGAGAGATATCAACCACCGGAGCATTAAAACCAGGACTGGTCTATGAAACTAGTACAACTGACTACTTAATCTACCTTTGTGGCCGAGGCTATAACCGGTCCACCATAAAGAGCATCGCAAATACTGTTCCTGATGGGTTTGATTGCCCCAAGGATTCTAATGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTGTCCGAATTGAAAGGCAGCGAAAGCAAGAAAATAGTGAGAACAGTTACAAATGTTGGTGGTGATGGTGCAACAGTTTACAAAGTCAGTGTGGACGCACCTGGGGAAGTAGATGTCAAAGTGATTCCAGAGAAATTGGAATTTTCAAAGAACAATCAGAAGCAGAGTTACAAAGTGGTGTTCACCTCAACTGTGTCCACACTGAAGAAAGAAGCCTTTGGTTCAATAACTTGGAGCAATGGAAAACATCGAGTCCGGAGTCCATTTGTGGTAACCAGTGAGAGTAGTAAGTCAGAAGATTAG

Coding sequence (CDS)

ATGCAAACCATTTCTTCTTGTGTCTTCCTTTTGGTTTGTGTCATCTTCTTGCTTCTTTCTGAAAATGCTAAAATAGCTGCTGCTGCAGCTGGGAACAATGGCGTTTACATCGTCTACATGGGATCCGCGTCCAGGACTGATTTCCTTCGCCTTCTTACCTCTGTCAACAGGCGGAATGGAAACGCAGTGGTACGGACTTATAAACATGGGTTCACAGGATTCGCAGCTCGTCTGTCAGAGCAGGAAGCCCATGCCATGAGACAAAGGCCTGGAGTTGTTTCGGTCTTCCCAGATCCAATACTGAAGCTCCACACAACTCATTCATGGGATTTCTTGGTCAGTCAGACAAGCGTCAAAATCGACAGCGCTCCCAAGGCAGATCCCCCGACCTCTTCCTCTCAGCCGTCCGATACCATTATCGGCATCTTGGACACCGGTATCTGGCCGGAATCCGAGAGTTTTAGAGACGAGGGTATGCCTCCGATACCGTCGAGGTGGAAAGGTACGTGCATGGCTGGCGATGACTTCACTTCCTCCAACTGTAACAGAAAAATCGTTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGGATAAGGTTCCACTCACCTCGAGACGGAGCTGGACATGGAACCCACGTGGCTTCAACAGCGGCCGGCAGTGCCGTGGCTAATGCATCCTACTATGGCCTCGCAGCCGGGACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTATAGAGTCTGTATGGCCGATGGTTGTCATGGATCGTCAATCCTGGCAGCGTTTGATGATGCCATTAACGATGGGGTCGATGTATTATCCTTATCTCTCGGTGCGCCCACCTACTACAAACCGGAATTGACGACCGACCCCATTGCCATCGGAGCCTTTCATGCAGTTGAGAAGGGGATTACAGTTGTCTGCTCTGCTGGAAACGACGGCCCCACTCCGGGATCGGTGGTGAACGACGCGCCGTGGATTCTAACAGTGGCTGCCTCCACCATTGATAGGGATTTTGAGTCTGATGTTGTGCTGGGCAACAAAAAAGTGATCAAGGGAGAAGGTATAAATTTCTCTGCTCTTGAAAGCTCTCCTGTATACCCACTGATACATGGCAAATCGGCCAACAAATCGAGTACCAGTGAAGACAGTGCGAGGATTTGTTCTGAAGGCTCCATGGACGAAGCTCTTGTGAAGGGGAAGATAGTTATTTGTGAGAGCAGTGAGGAAGGAGGCGGTTCCTATTGGCAAGGTCAGGCTGAGACAGTGGAGAGCCTTGGAGGGGTCGGGATAGTTCTCATTGATGACCAAACAAAGCTAGTGGCAGAGAAATTTACTTCTCCCATCACCGCTATTAGCTCAAAGGATGGCGATGAGGTCCTTGCCTATGTTCACTCAAGCAGGAACCCAGTTGCTACAATCCTGCCCACCCTGACCGTAATAAACTATAAGCCAGCGCCCGCTATGGCATACTTTTCATCCAGAGGGCCCAATCCCGCAGTATTAAACATGATCAAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCCTGGCTTGGAAATGACACGAACTCTACTCCGCAACCAGAAAATTCGCCGCTCTTCAATGTGATCTCAGGAACTTCAATGTCCTGCCCGCATGTCTCCGGCGTAGTAGCCATGCAATTTTCTCACGAAAACTTCACAGCAATCCAAACGAATAACTTGGAATCGCCGATGACTTTGGATACGGGATCAGTAGCTACACCTTATGATTATGGGGCAGGAGAGATATCAACCACCGGAGCATTAAAACCAGGACTGGTCTATGAAACTAGTACAACTGACTACTTAATCTACCTTTGTGGCCGAGGCTATAACCGGTCCACCATAAAGAGCATCGCAAATACTGTTCCTGATGGGTTTGATTGCCCCAAGGATTCTAATGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTGTCCGAATTGAAAGGCAGCGAAAGCAAGAAAATAGTGAGAACAGTTACAAATGTTGGTGGTGATGGTGCAACAGTTTACAAAGTCAGTGTGGACGCACCTGGGGAAGTAGATGTCAAAGTGATTCCAGAGAAATTGGAATTTTCAAAGAACAATCAGAAGCAGAGTTACAAAGTGGTGTTCACCTCAACTGTGTCCACACTGAAGAAAGAAGCCTTTGGTTCAATAACTTGGAGCAATGGAAAACATCGAGTCCGGAGTCCATTTGTGGTAACCAGTGAGAGTAGTAAGTCAGAAGATTAG

Protein sequence

MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSNCNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLFNVISGTSMSCPHVSGVVAMQFSHENFTAIQTNNLESPMTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED
Homology
BLAST of Carg08662 vs. NCBI nr
Match: KAG7024139.1 (CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1494.6 bits (3868), Expect = 0.0e+00
Identity = 760/760 (100.00%), Postives = 760/760 (100.00%), Query Frame = 0

Query: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60
           MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG
Sbjct: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60

Query: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120
           NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI
Sbjct: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120

Query: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180
           DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN
Sbjct: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180

Query: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240
           CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS
Sbjct: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240

Query: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300
           PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF
Sbjct: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300

Query: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360
           HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN
Sbjct: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360

Query: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420
           FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ
Sbjct: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420

Query: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480
           GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT
Sbjct: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480

Query: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540
           LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF
Sbjct: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540

Query: 541 NVISGTSMSCPHVSGVVAMQFSHENFTAIQTNNLESPMTLDTGSVATPYDYGAGEISTTG 600
           NVISGTSMSCPHVSGVVAMQFSHENFTAIQTNNLESPMTLDTGSVATPYDYGAGEISTTG
Sbjct: 541 NVISGTSMSCPHVSGVVAMQFSHENFTAIQTNNLESPMTLDTGSVATPYDYGAGEISTTG 600

Query: 601 ALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYISNMNYPTIA 660
           ALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYISNMNYPTIA
Sbjct: 601 ALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYISNMNYPTIA 660

Query: 661 VSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVV 720
           VSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVV
Sbjct: 661 VSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVV 720

Query: 721 FTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           FTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED
Sbjct: 721 FTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 760

BLAST of Carg08662 vs. NCBI nr
Match: KAG6591258.1 (CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1466.4 bits (3795), Expect = 0.0e+00
Identity = 752/772 (97.41%), Postives = 754/772 (97.67%), Query Frame = 0

Query: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60
           MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG
Sbjct: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60

Query: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120
           NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI
Sbjct: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120

Query: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180
           DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN
Sbjct: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180

Query: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240
           CNRKI+GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS
Sbjct: 181 CNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240

Query: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300
           PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF
Sbjct: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300

Query: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360
           HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN
Sbjct: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360

Query: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420
           FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ
Sbjct: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420

Query: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480
           GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT
Sbjct: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480

Query: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540
           LTVINYKPAPA+AYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF
Sbjct: 481 LTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540

Query: 541 NVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTGSVATP 600
           NVISGTSMSCPHVSGVVAM  S                TAIQTNNLESPMTLDTGSVATP
Sbjct: 541 NVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGSVATP 600

Query: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660
           YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA
Sbjct: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660

Query: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720
           DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF
Sbjct: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720

Query: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED
Sbjct: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 772

BLAST of Carg08662 vs. NCBI nr
Match: XP_022937005.1 (CO(2)-response secreted protease [Cucurbita moschata])

HSP 1 Score: 1462.2 bits (3784), Expect = 0.0e+00
Identity = 750/772 (97.15%), Postives = 752/772 (97.41%), Query Frame = 0

Query: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60
           MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG
Sbjct: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60

Query: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120
           NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI
Sbjct: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120

Query: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180
           DSAPKADPPTSSSQP DTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN
Sbjct: 121 DSAPKADPPTSSSQPFDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180

Query: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240
           CNRKI+GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS
Sbjct: 181 CNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240

Query: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300
           PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAP YYKPELTTDPIAIGAF
Sbjct: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPNYYKPELTTDPIAIGAF 300

Query: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360
           HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN
Sbjct: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360

Query: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420
           FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ
Sbjct: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420

Query: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480
           GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT
Sbjct: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480

Query: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540
           LTVINYKPAPA+AYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF
Sbjct: 481 LTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540

Query: 541 NVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTGSVATP 600
           NVISGTSMSCPHVSGVVAM  S                TAIQTNNLESPMTLDTGSVATP
Sbjct: 541 NVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGSVATP 600

Query: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660
           YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA
Sbjct: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660

Query: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720
           DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF
Sbjct: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720

Query: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED
Sbjct: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 772

BLAST of Carg08662 vs. NCBI nr
Match: XP_022975820.1 (CO(2)-response secreted protease [Cucurbita maxima])

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 742/772 (96.11%), Postives = 748/772 (96.89%), Query Frame = 0

Query: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60
           MQTISSC FLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG
Sbjct: 1   MQTISSCAFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60

Query: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120
           NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI
Sbjct: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120

Query: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180
           DSAPK++PPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN
Sbjct: 121 DSAPKSEPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180

Query: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240
           CNRKI+GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS
Sbjct: 181 CNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240

Query: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300
           PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLG P +YKPEL TDPIAIGAF
Sbjct: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGTPYFYKPELATDPIAIGAF 300

Query: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360
           HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN
Sbjct: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360

Query: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420
           FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ
Sbjct: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420

Query: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480
           GQAETVESLGGVGIVLIDDQ KLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT
Sbjct: 421 GQAETVESLGGVGIVLIDDQAKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480

Query: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540
           LTVINYKPAPA+AYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF
Sbjct: 481 LTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540

Query: 541 NVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTGSVATP 600
           NVISGTSMSCPHVSGVVAM  S                TAIQTNNLESPMTLDTGSVATP
Sbjct: 541 NVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGSVATP 600

Query: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660
           YDYGAGEISTT ALKPGLVYETSTTDYLIYLCGRGYNRSTIKSI+NTVPDGFDCPKDSNA
Sbjct: 601 YDYGAGEISTTEALKPGLVYETSTTDYLIYLCGRGYNRSTIKSISNTVPDGFDCPKDSNA 660

Query: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720
           DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF
Sbjct: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720

Query: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED
Sbjct: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 772

BLAST of Carg08662 vs. NCBI nr
Match: XP_023535470.1 (CO(2)-response secreted protease [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 741/772 (95.98%), Postives = 750/772 (97.15%), Query Frame = 0

Query: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60
           MQTISS +FL VCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTD+LRLLTSVNRRNG
Sbjct: 1   MQTISSWLFLWVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDYLRLLTSVNRRNG 60

Query: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120
           NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGV+SVFPDPILKLHTTHSWDFLVSQTSVKI
Sbjct: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVISVFPDPILKLHTTHSWDFLVSQTSVKI 120

Query: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180
           DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN
Sbjct: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180

Query: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240
           CNRKI+GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS
Sbjct: 181 CNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240

Query: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300
           PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLG P YYKPELTTDPIAIGAF
Sbjct: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGVPYYYKPELTTDPIAIGAF 300

Query: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360
           HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN
Sbjct: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360

Query: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420
           FSALESSPVYPLIHGKSANKSS+SEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ
Sbjct: 361 FSALESSPVYPLIHGKSANKSSSSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420

Query: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480
           GQAETVESLGGVG+VLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT
Sbjct: 421 GQAETVESLGGVGVVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480

Query: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540
           LTVINYKPAPA+AYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF
Sbjct: 481 LTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540

Query: 541 NVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTGSVATP 600
           NVISGTSMSCPHVSGVVAM  S                TAIQTNNLESPMTLDTGSVATP
Sbjct: 541 NVISGTSMSCPHVSGVVAMVKSKNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGSVATP 600

Query: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660
           YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSI+NTVPDGFDCPKDSNA
Sbjct: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSISNTVPDGFDCPKDSNA 660

Query: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720
           DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF
Sbjct: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720

Query: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESS+SED
Sbjct: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSRSED 772

BLAST of Carg08662 vs. ExPASy Swiss-Prot
Match: Q9LNU1 (CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 5.2e-243
Identity = 437/766 (57.05%), Postives = 570/766 (74.41%), Query Frame = 0

Query: 11  LVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLR----LLTSVNRRNGNAVVRT 70
           L+C++F+  +E     A +   +GVYIVYMGSAS          L+ ++ +R  N ++ T
Sbjct: 16  LLCILFMTETE-----AGSRNGDGVYIVYMGSASSAANANRAQILINTMFKRRANDLLHT 75

Query: 71  YKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDSAPKA 130
           YKHGF+GFAARL+ +EA  + ++PGVVSVFPDP  +LHTTHSWDFL  QTSVK+DS P  
Sbjct: 76  YKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDSGP-- 135

Query: 131 DPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSNCNRKIV 190
            P ++S    D+I+GILDTGIWPESESF D+ M PIPSRWKGTCM   DF SSNCNRKI+
Sbjct: 136 -PSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCNRKII 195

Query: 191 GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIA 250
           GAR+Y++ + D   +++ RD  GHG+HV+ST AGSAV NASYYG+A+GTAKGGS  +RIA
Sbjct: 196 GARYYKNPDDDS-EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIA 255

Query: 251 MYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAFHAVEKG 310
           MY+VC   GC GSSILAAFDDAI DGVDVLSLSLGAP Y + +L TDPIAIGAFHAVE+G
Sbjct: 256 MYKVCNPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQG 315

Query: 311 ITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINFSALES 370
           I V+CSAGNDGP  G+V N APWI+TVAA+TIDRDFESDVVLG  KVIKGEGI+FS +  
Sbjct: 316 ILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFSNVSK 375

Query: 371 SPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQGQA-ET 430
           SPVYPLIHGKSA  +  SE SAR C   S+D+  VKGKIV+CE+    GGSY+   A + 
Sbjct: 376 SPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCENV---GGSYYASSARDE 435

Query: 431 VESLGGVGIVLIDDQTKLVAEKFTS-PITAISSKDGDEVLAYVHSSRNPVATILPTLTVI 490
           V+S GG G V +DD+T+ VA  + S P T I SK+  E+ +Y++S+++PVATILPT TV 
Sbjct: 436 VKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPTATVE 495

Query: 491 NYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLFNVIS 550
            + PAPA+AYFSSRGP+    +++KPDI+APGV+ILAAW GND++ + + + +  +NVIS
Sbjct: 496 KFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWTGNDSSISLEGKPASQYNVIS 555

Query: 551 GTSMSCPHVSGVVAM-QFSHENF-----------TAIQTNNLESPMTLDTGSVATPYDYG 610
           GTSM+ PHVS V ++ +  H  +           TA QTNN +  +T +TG+ ATPYD G
Sbjct: 556 GTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTTATQTNNDKGLITTETGATATPYDSG 615

Query: 611 AGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYIS 670
           AGE+S+T +++PGLVYET+ TDYL +LC  GYN +TIK+++   P+ F CP DSN D IS
Sbjct: 616 AGELSSTASMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFPENFTCPADSNLDLIS 675

Query: 671 NMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKNN 730
            +NYP+I +S  KG+ SK + RTVTNVG DG  VY VSV+ P   +++V PEKL+F+K+ 
Sbjct: 676 TINYPSIGISGFKGNGSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEKLQFTKDG 735

Query: 731 QKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKS 759
           +K +Y+V+ ++T S LK++ FG++TWSN K++VRSP V++SESS++
Sbjct: 736 EKLTYQVIVSATAS-LKQDVFGALTWSNAKYKVRSPIVISSESSRT 768

BLAST of Carg08662 vs. ExPASy Swiss-Prot
Match: F4HSQ2 (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 9.5e-205
Identity = 387/787 (49.17%), Postives = 526/787 (66.84%), Query Frame = 0

Query: 3   TISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASR-----TDFLRLLTSVNR 62
           TI   +F  + VI    SE +K        +G YI+YMG+AS       D + LL+S+ +
Sbjct: 8   TIMFFMFFFLSVIQKCKSETSK--------SGDYIIYMGAASSDGSTDNDHVELLSSLLQ 67

Query: 63  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 122
           R+G   +  YKHGF+GFAA LSE EAH + ++PGV+SVFPD +L+LHTT SWDFLV ++ 
Sbjct: 68  RSGKTPMHRYKHGFSGFAAHLSEDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLVQESY 127

Query: 123 VKIDSAPKAD-PPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 182
            +     + +    S     DTIIG LD+GIWPE++SF D  M P+P +WKGTCM G   
Sbjct: 128 QRDTYFTEMNYEQESEMHEGDTIIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGKKT 187

Query: 183 --TSSNCNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAG 242
              S  CNRK++GAR+Y SS      + +PRD  GHGTHVAS AAG  +ANASYYGLA+G
Sbjct: 188 QPDSFRCNRKLIGARYYNSSFFLDPDYETPRDFLGHGTHVASIAAGQIIANASYYGLASG 247

Query: 243 TAKGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDP 302
             +GGSP SRIAMYR C   GC GSSILAAFDDAI DGVDV+S+S+G    +   L  DP
Sbjct: 248 IMRGGSPSSRIAMYRACSLLGCRGSSILAAFDDAIADGVDVISISMG---LWPDNLLEDP 307

Query: 303 IAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLG--NKK 362
           ++IG+FHAVE+GITVVCS GN GP+  SV N APW++TVAASTIDR FES+++LG    +
Sbjct: 308 LSIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENR 367

Query: 363 VIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSE 422
           +I+G GIN + ++ +  YPLIH +SA K   +E++AR C+  ++D+ +VKGKIV+C+S  
Sbjct: 368 LIEGFGINIANIDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDSDL 427

Query: 423 EGGGSYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSP---ITAISSKDGDEVLAYVHS 482
           +     W  +++ V+ LGG+G+VL+DD++  ++  F  P   +T I  +DG ++++Y++S
Sbjct: 428 DNQVIQW--KSDEVKRLGGIGMVLVDDESMDLS--FIDPSFLVTIIKPEDGIQIMSYINS 487

Query: 483 SRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTN 542
           +R P+ATI+PT +   +  AP++  FSSRGP     +++KPDI+APGVNILA+WL  D N
Sbjct: 488 TREPIATIMPTRSRTGHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWLVGDRN 547

Query: 543 STPQPENSPLFNVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESP 602
           + P+ +  PLFN+ SGTSMSCPHVSG+ A   S                TA+Q  N  S 
Sbjct: 548 AAPEGKPPPLFNIESGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQMTNTGSH 607

Query: 603 MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVP 662
           +T +TG  ATPYD+GAG+++  G   PGL+YET+  DYL +L   G+    IK I+N +P
Sbjct: 608 ITTETGEKATPYDFGAGQVTIFGPSSPGLIYETNHMDYLNFLGYYGFTSDQIKKISNRIP 667

Query: 663 DGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVG----GDGATVYKVSVDA 722
            GF CP+ SN   ISN+NYP+I++S   G ES+++ RTVTNV     GD  TVY VS+DA
Sbjct: 668 QGFACPEQSNRGDISNINYPSISISNFNGKESRRVSRTVTNVASRLIGDEDTVYTVSIDA 727

Query: 723 PGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTS 761
           P  + V+VIP +L F K   K SY+V+F+ST + LK +AFGSITWSNG + VRSPFVVTS
Sbjct: 728 PEGLLVRVIPRRLHFRKIGDKLSYQVIFSSTTTILKDDAFGSITWSNGMYNVRSPFVVTS 779

BLAST of Carg08662 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 5.5e-152
Identity = 319/746 (42.76%), Postives = 443/746 (59.38%), Query Frame = 0

Query: 36  YIVYMGSASRT----------DFLRLLTSVNRRNGNAVVRTYKHGFTGFAARLSEQEAHA 95
           YIVYMG  S            + L  +T        A +  Y   F GF+A ++ ++A  
Sbjct: 28  YIVYMGDRSHPNSESVVRANHEILASVTGSLNDAKAAAIHHYSRSFQGFSAMITPEQAKK 87

Query: 96  MRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDSAPKADPPTSSSQPSDTIIGILDT 155
           +     VVSVF   + KLHTTHSWDFL   T  K       + P++    S+ I+G++D+
Sbjct: 88  LADHNSVVSVFESKMNKLHTTHSWDFLGLDTVYK-------NNPSALDSASNVIVGVIDS 147

Query: 156 GIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSNCNRKIVGARFYESSES--------- 215
           G+WPESESF D G+ P+P ++KG C+ GD+FT +NCN+KI+GARFY              
Sbjct: 148 GVWPESESFNDYGLGPVPEKFKGECVTGDNFTLANCNKKIIGARFYSKGLEAEIGPLENI 207

Query: 216 -DGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADG 275
            D I F SPRD  GHGTH AST AGS V+N S +G+A GTA+GG+P +R+++Y+ C    
Sbjct: 208 VDSIFFRSPRDSDGHGTHTASTIAGSIVSNVSLFGMAKGTARGGAPSARLSIYKACWFGF 267

Query: 276 CHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAFHAVEKGITVVCSAGN 335
           C  + + AA DDAI+DGVD+LSLSLG P   +P    + I++GAFHA +KGI V  SAGN
Sbjct: 268 CSDADVFAAMDDAIHDGVDILSLSLG-PDPPQPLYFENAISVGAFHAFQKGILVSASAGN 327

Query: 336 DGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINFSALESSPVYPLIHG 395
               P +  N APWI TVAAST+DR+F SD+ LGN KV+KG  +N   +E S  Y LI+G
Sbjct: 328 -SVFPRTACNVAPWIFTVAASTVDREFRSDIYLGNSKVLKGLSLNPIKMEGS--YGLIYG 387

Query: 396 KSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQGQAETVESLGGVGIV 455
            +A  +  +  +A  C E ++D  L+KGKIVIC  + E      + +A  ++  GGVG++
Sbjct: 388 SAAAAAGDAALNASFCKEHTLDPTLIKGKIVIC--TVEKFTDNRREKAIIIKQGGGVGMI 447

Query: 456 LIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPTLTVINYKPAPAMAYF 515
           LID   + V  +F  P T I     +E+ AY+ + +NP ATI PTLT++  KPAP  A F
Sbjct: 448 LIDHNARDVGFQFVIPSTMIGQDAVEELQAYMKTEKNPTATIFPTLTLVGTKPAPESAAF 507

Query: 516 SSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLFNVISGTSMSCPHVSG 575
           SS GPN    ++IKPDI+ PGVNILAAW    T +T + + S  +N+ISGTSMSCPH+S 
Sbjct: 508 SSVGPNIITPDIIKPDITGPGVNILAAWSPVATEATVE-QKSVNYNIISGTSMSCPHISA 567

Query: 576 VVAMQFSHE---NFTAIQTNNLESPMTLD----------TGSVATPYDYGAGEISTTGAL 635
           + A+  SH    +  AI +  + S   +D           G+ ATP+DYG+G ++   +L
Sbjct: 568 ISAIIKSHHPSWSPAAIMSAIMTSATVMDNTHSLIGRDPNGTQATPFDYGSGHVNPVASL 627

Query: 636 KPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYISNMNYPTIAVS 695
            PGLVY+ S+ D L +LC  G + + +K++   +     C K   A Y  N NYP+I VS
Sbjct: 628 NPGLVYDFSSQDVLNFLCSNGASPAQLKNLTGELT---QCQKSPTASY--NFNYPSIGVS 687

Query: 696 ELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVVFT 749
            L GS S  + RTVT  G +  T Y  SV+ P  V V+V P KL+F K  +K ++++ FT
Sbjct: 688 NLNGSLS--VYRTVTYYGQE-PTEYFASVERPSGVIVRVTPAKLKFWKAGEKITFRIDFT 747

BLAST of Carg08662 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.4e-139
Identity = 313/784 (39.92%), Postives = 443/784 (56.51%), Query Frame = 0

Query: 14  VIFLLLSENAKIAAAAAGNNGVYIVYMGSASRT----------------DFLRLLTSVNR 73
           ++ LLL   +     A+ ++  Y+VY G+ S                  DFL   T    
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 74  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 133
           R  +A+  +Y     GFAA L    A+ + + P VVSVFP+  LKLHTT SWDFL  + +
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 134 VKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFT 193
             +   P +     +    DTII  LDTG+WPES+SFRDEG+ PIPSRWKG C    D T
Sbjct: 130 SYV---PSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 189

Query: 194 SSNCNRKIVGARFYESSESDGI-----RFHSPRDGAGHGTHVASTAAGSAVANASYYGLA 253
             +CNRK++GAR++    +  +      F SPRD  GHG+H  STAAG  V   S +G  
Sbjct: 190 -FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQG 249

Query: 254 AGTAKGGSPGSRIAMYRVC----MADGCHGSSILAAFDDAINDGVDVLSLSLGA-PTYYK 313
            GTAKGGSP +R+A Y+VC      + C+ + +LAAFD AI+DG DV+S+SLG  PT + 
Sbjct: 250 NGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF- 309

Query: 314 PELTTDPIAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVV 373
                D +AIG+FHA +K I VVCSAGN GP   +V N APW +TV AST+DR+F S++V
Sbjct: 310 ---FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLV 369

Query: 374 LGNKKVIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVI 433
           LGN K  KG+ ++ +AL  +  YP++   +A   + S   A++C  GS+D    KGKI++
Sbjct: 370 LGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILV 429

Query: 434 CESSEEGGGSYWQGQAETVESLGGVGIVLIDDQ---TKLVAEKFTSPITAISSKDGDEVL 493
           C   + G       +   V   GG+G+VL +       L+A+    P T ++SKD   V 
Sbjct: 430 CLRGQNGRVE----KGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVS 489

Query: 494 AYVHSSRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWL 553
            Y+  ++ P+A I P+ T +  KPAP MA FSS+GP+     ++KPDI+APGV+++AA+ 
Sbjct: 490 RYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYT 549

Query: 554 G--NDTNSTPQPENSPLFNVISGTSMSCPHVSGVVAM---QFSHENFTAIQT-------- 613
           G  + TN    P    LFN ISGTSMSCPH+SG+  +   ++   +  AI++        
Sbjct: 550 GAVSPTNEQFDPRRL-LFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATI 609

Query: 614 -NNLESPMTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIK 673
            +++  P+   T   ATP+ +GAG +    A+ PGLVY+    DYL +LC  GYN S I 
Sbjct: 610 MDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQIS 669

Query: 674 SIANTVPDGFDC--PKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYK 733
             +    + F C  PK S    + N+NYP+I V  L  S+   + RTV NVG    ++Y 
Sbjct: 670 VFSG---NNFTCSSPKIS----LVNLNYPSITVPNLTSSK-VTVSRTVKNVG--RPSMYT 729

Query: 734 VSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKK-EAFGSITWSNGKHRVRS 752
           V V+ P  V V V P  L F+K  +++++KV+   +   + K   FG + WS+ KHRVRS
Sbjct: 730 VKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRS 770

BLAST of Carg08662 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.7e-137
Identity = 316/784 (40.31%), Postives = 449/784 (57.27%), Query Frame = 0

Query: 5   SSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRL--------LTSVN 64
           S+  FLL+C+ F  +S       +++ + G YIV+M  +       L        L S++
Sbjct: 8   STAFFLLLCLGFCHVS-------SSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 67

Query: 65  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 124
             +   ++ TY++   GF+ RL+++EA ++  +PGV+SV P+   +LHTT +  FL    
Sbjct: 68  --DSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 127

Query: 125 SVKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 184
                    AD    +   SD ++G+LDTG+WPES+S+ DEG  PIPS WKG C AG +F
Sbjct: 128 HT-------ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNF 187

Query: 185 TSSNCNRKIVGARF----YESSE---SDGIRFHSPRDGAGHGTHVASTAAGSAVANASYY 244
           T+S CNRK++GARF    YES+     +     SPRD  GHGTH +STAAGS V  AS  
Sbjct: 188 TASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 247

Query: 245 GLAAGTAKGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGA--PTYYK 304
           G A+GTA+G +P +R+A+Y+VC   GC  S ILAA D AI D V+VLS+SLG     YY+
Sbjct: 248 GYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 307

Query: 305 PELTTDPIAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVV 364
                D +AIGAF A+E+GI V CSAGN GP+  S+ N APWI TV A T+DRDF +  +
Sbjct: 308 -----DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 367

Query: 365 LGNKKVIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVI 424
           LGN K   G  +         + P I+  +A+ ++    +  +C  G++    VKGKIV+
Sbjct: 368 LGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT----NGNLCMTGTLIPEKVKGKIVM 427

Query: 425 CESSEEGGGSYWQGQAETVESLGGVGIVLID---DQTKLVAEKFTSPITAISSKDGDEVL 484
           C+     G +    + + V++ GGVG++L +   +  +LVA+    P T +  K GD + 
Sbjct: 428 CDR----GINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 487

Query: 485 AYVHSSRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWL 544
            YV +  NP A+I    TV+  KP+P +A FSSRGPN    N++KPD+ APGVNILAAW 
Sbjct: 488 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 547

Query: 545 G-NDTNSTPQPENSPLFNVISGTSMSCPHVSGVVAMQFS-HENF-----------TAIQT 604
           G               FN+ISGTSMSCPHVSG+ A+  S H  +           TA +T
Sbjct: 548 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 607

Query: 605 NNLESP-MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIK 664
                P + + TG  +TP+D+GAG +S T A  PGL+Y+ +T DYL +LC   Y    I+
Sbjct: 608 YKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR 667

Query: 665 SIANTVPDGFDCPKDSNADY-ISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKV 724
           S++      + C  D +  Y ++++NYP+ AV+ + G  + K  RTVT+VGG G    KV
Sbjct: 668 SVSRR---NYTC--DPSKSYSVADLNYPSFAVN-VDGVGAYKYTRTVTSVGGAGTYSVKV 727

Query: 725 SVDAPGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLK-KEAFGSITWSNGKHRVRSP 753
           + +  G V + V P  L F + N+K+SY V FT   S      +FGSI WS+GKH V SP
Sbjct: 728 TSETTG-VKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSP 755

BLAST of Carg08662 vs. ExPASy TrEMBL
Match: A0A6J1FFB5 (CO(2)-response secreted protease OS=Cucurbita moschata OX=3662 GN=LOC111443427 PE=3 SV=1)

HSP 1 Score: 1462.2 bits (3784), Expect = 0.0e+00
Identity = 750/772 (97.15%), Postives = 752/772 (97.41%), Query Frame = 0

Query: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60
           MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG
Sbjct: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60

Query: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120
           NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI
Sbjct: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120

Query: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180
           DSAPKADPPTSSSQP DTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN
Sbjct: 121 DSAPKADPPTSSSQPFDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180

Query: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240
           CNRKI+GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS
Sbjct: 181 CNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240

Query: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300
           PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAP YYKPELTTDPIAIGAF
Sbjct: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPNYYKPELTTDPIAIGAF 300

Query: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360
           HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN
Sbjct: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360

Query: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420
           FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ
Sbjct: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420

Query: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480
           GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT
Sbjct: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480

Query: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540
           LTVINYKPAPA+AYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF
Sbjct: 481 LTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540

Query: 541 NVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTGSVATP 600
           NVISGTSMSCPHVSGVVAM  S                TAIQTNNLESPMTLDTGSVATP
Sbjct: 541 NVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGSVATP 600

Query: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660
           YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA
Sbjct: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660

Query: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720
           DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF
Sbjct: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720

Query: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED
Sbjct: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 772

BLAST of Carg08662 vs. ExPASy TrEMBL
Match: A0A6J1IHT1 (CO(2)-response secreted protease OS=Cucurbita maxima OX=3661 GN=LOC111476411 PE=3 SV=1)

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 742/772 (96.11%), Postives = 748/772 (96.89%), Query Frame = 0

Query: 1   MQTISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60
           MQTISSC FLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG
Sbjct: 1   MQTISSCAFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRLLTSVNRRNG 60

Query: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120
           NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI
Sbjct: 61  NAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKI 120

Query: 121 DSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180
           DSAPK++PPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN
Sbjct: 121 DSAPKSEPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSN 180

Query: 181 CNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240
           CNRKI+GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS
Sbjct: 181 CNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGS 240

Query: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAF 300
           PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLG P +YKPEL TDPIAIGAF
Sbjct: 241 PGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGTPYFYKPELATDPIAIGAF 300

Query: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360
           HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN
Sbjct: 301 HAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGIN 360

Query: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420
           FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ
Sbjct: 361 FSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQ 420

Query: 421 GQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480
           GQAETVESLGGVGIVLIDDQ KLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT
Sbjct: 421 GQAETVESLGGVGIVLIDDQAKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVATILPT 480

Query: 481 LTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540
           LTVINYKPAPA+AYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF
Sbjct: 481 LTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLF 540

Query: 541 NVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTGSVATP 600
           NVISGTSMSCPHVSGVVAM  S                TAIQTNNLESPMTLDTGSVATP
Sbjct: 541 NVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGSVATP 600

Query: 601 YDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNA 660
           YDYGAGEISTT ALKPGLVYETSTTDYLIYLCGRGYNRSTIKSI+NTVPDGFDCPKDSNA
Sbjct: 601 YDYGAGEISTTEALKPGLVYETSTTDYLIYLCGRGYNRSTIKSISNTVPDGFDCPKDSNA 660

Query: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720
           DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF
Sbjct: 661 DYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEF 720

Query: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED
Sbjct: 721 SKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 772

BLAST of Carg08662 vs. ExPASy TrEMBL
Match: A0A0A0LJ71 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873800 PE=3 SV=1)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 634/774 (81.91%), Postives = 686/774 (88.63%), Query Frame = 0

Query: 1   MQTISSCVFL-LVCVIFLLLSENAKIA-AAAAGNNGVYIVYMGSAS---RTDFLRLLTSV 60
           MQ+IS CVF  LVCV F L+SEN K+A AA    NGVYIVYMGSAS   RTDFLRLL SV
Sbjct: 1   MQSISFCVFFALVCVTFFLVSENVKVADAAEDARNGVYIVYMGSASSGFRTDFLRLLNSV 60

Query: 61  NRRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQ 120
           NRR  NAVV TYKHGFTGFAA LSE EA AMRQ PGVVSVFPDP+LKLHTTHSWDFLVSQ
Sbjct: 61  NRR--NAVVHTYKHGFTGFAAHLSEHEAQAMRQSPGVVSVFPDPLLKLHTTHSWDFLVSQ 120

Query: 121 TSVKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDD 180
           TSVKID+ PK+DPP SSSQP DTIIGILDTGIWPESESF D GM PIPSRWKGTCM GDD
Sbjct: 121 TSVKIDANPKSDPPASSSQPYDTIIGILDTGIWPESESFNDMGMGPIPSRWKGTCMTGDD 180

Query: 181 FTSSNCNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGT 240
           FTSSNCNRKI+GARFYESSESDGIR+HSPRDGAGHGTHVASTAAGSAVANASYYGLAAGT
Sbjct: 181 FTSSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGT 240

Query: 241 AKGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPI 300
           AKGGSPGSRIAMYRVCMADGC GSSI+ AFDD+I DGVDVLSLSLG P+ ++P+LT DPI
Sbjct: 241 AKGGSPGSRIAMYRVCMADGCRGSSIMKAFDDSIADGVDVLSLSLGTPSVFRPDLTADPI 300

Query: 301 AIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIK 360
           AIGAFHAVEKGITVVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGNKKVIK
Sbjct: 301 AIGAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIK 360

Query: 361 GEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGG 420
           GEGINFS L+ SPVYPLI GKSA K+S SEDSARICSE SMDEA VKGKIVICE+S EGG
Sbjct: 361 GEGINFSDLQKSPVYPLIEGKSAKKASDSEDSARICSEDSMDEAQVKGKIVICENSVEGG 420

Query: 421 GSYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVA 480
           GS WQ QAETV++LGGVG+VLIDD +KLVAEKF++P+T IS KDG E+L+YV+SSR PVA
Sbjct: 421 GSDWQSQAETVKNLGGVGLVLIDDDSKLVAEKFSTPMTVISKKDGLEILSYVNSSRKPVA 480

Query: 481 TILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPE 540
           T+LPT T+INYKPAPA+ YFSSRGPNPAVLN+IKPDISAPGVNILAAWLGND++STPQ  
Sbjct: 481 TVLPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQAT 540

Query: 541 NSPLFNVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTG 600
            SPLFNVISGTSMSCPHVSGVVA   S                TAIQTNNL SPMTLDTG
Sbjct: 541 KSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIRSAIMTTAIQTNNLGSPMTLDTG 600

Query: 601 SVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCP 660
           SVATPYDYGAGEIST GAL+PGLVYETSTTDYL+YLCGRGYN +TIKSI  T+PDGFDCP
Sbjct: 601 SVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYNLTTIKSITTTIPDGFDCP 660

Query: 661 KDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIP 720
           K+SNADYISNMNYPTIAVSELKG ESKK++RTVTNVGG+G TVY VSVDAP EV+VKVIP
Sbjct: 661 KNSNADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPQEVEVKVIP 720

Query: 721 EKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSK 758
           EKL+F+KN +KQSY+VVFT TVST+K+  FGSITW+NGKHRVRSPFVVTSESS+
Sbjct: 721 EKLKFAKNYEKQSYQVVFTPTVSTMKR-GFGSITWTNGKHRVRSPFVVTSESSE 771

BLAST of Carg08662 vs. ExPASy TrEMBL
Match: A0A6J1C938 (CO(2)-response secreted protease OS=Momordica charantia OX=3673 GN=LOC111009346 PE=3 SV=1)

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 618/769 (80.36%), Postives = 664/769 (86.35%), Query Frame = 0

Query: 8   VFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMG--SASRTDFLRLLTSVNRRNGNAVVR 67
           VFL   VIF L+S + K  AA AGNNGVYIVYMG  SASRTDFLRLL+SVNRRN NAVV 
Sbjct: 9   VFLFASVIF-LVSVHGK-TAAEAGNNGVYIVYMGTASASRTDFLRLLSSVNRRNRNAVVH 68

Query: 68  TYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDSAP- 127
           TYKHGF+GFAARLSE+E   MRQ PGVVSVFPDP+LKLHTTHSWDFLVSQTSVKID+ P 
Sbjct: 69  TYKHGFSGFAARLSEKETQEMRQTPGVVSVFPDPLLKLHTTHSWDFLVSQTSVKIDANPT 128

Query: 128 KADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSNCNRK 187
           K+DPP SSSQP D+IIGILDTGIWPESESF D GM PIP RWKGTCM G+DFTSSNCN K
Sbjct: 129 KSDPPASSSQPYDSIIGILDTGIWPESESFNDNGMGPIPLRWKGTCMVGNDFTSSNCNTK 188

Query: 188 IVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSR 247
           ++GARFYESS+SD IRFHSPRD AGHGTHVASTAAGSA ANASYYGLAAGTAKGGSPGSR
Sbjct: 189 LIGARFYESSDSDEIRFHSPRDEAGHGTHVASTAAGSAAANASYYGLAAGTAKGGSPGSR 248

Query: 248 IAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAFHAVE 307
           IAMYRVC  DGC GS+I+AAFDDAI DGVDVLSLSLG+P Y++ E   DPIAIGAFHAVE
Sbjct: 249 IAMYRVCGTDGCRGSAIMAAFDDAIADGVDVLSLSLGSPYYFRQETKDDPIAIGAFHAVE 308

Query: 308 KGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINFSAL 367
           KGI VVCSAGNDGPT GSVVNDAPWILTVAASTIDRDFESDVVLGN KVIKGEGINFSAL
Sbjct: 309 KGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRDFESDVVLGNNKVIKGEGINFSAL 368

Query: 368 ESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQGQAE 427
           + +P YPLI GKSA K+  SEDSARICSE SMDEALVKGKIVICESS EGGGS WQ Q E
Sbjct: 369 KKTPEYPLIQGKSAKKADASEDSARICSEDSMDEALVKGKIVICESSVEGGGSDWQNQIE 428

Query: 428 TVESLGGVGIVLIDDQTKLVAEKFT-SPITAISSKDGDEVLAYVHSSRNPVATILPTLTV 487
           TV+ L G+G+VLIDD+ KLVAEKF  S ITAIS KD  EVL+Y +SSRNP ATILPT+TV
Sbjct: 429 TVKGLEGIGMVLIDDRAKLVAEKFDGSNITAISKKDSAEVLSYANSSRNPTATILPTVTV 488

Query: 488 INYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLFNVI 547
           INYKPAPA+AYFSSRGPNPA+LN+IKPDISAPGVNILAAWLGND+NSTPQ +  PLFNVI
Sbjct: 489 INYKPAPAVAYFSSRGPNPAILNIIKPDISAPGVNILAAWLGNDSNSTPQGKKPPLFNVI 548

Query: 548 SGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESPMTLDTGSVATPYDY 607
           SGTSMSCPHVSGVV +  S                TAIQTNNL SPMTLDTGSVATPYDY
Sbjct: 549 SGTSMSCPHVSGVVGLLRSKNPSWSPSAIKSAIMTTAIQTNNLGSPMTLDTGSVATPYDY 608

Query: 608 GAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYI 667
           GAGEISTTGAL+PGLVYET  TDYL YLC RGYN S IKSI+ TVPDGFDCPK   ADYI
Sbjct: 609 GAGEISTTGALQPGLVYETDITDYLNYLCSRGYNLSIIKSISKTVPDGFDCPKKPTADYI 668

Query: 668 SNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKN 727
           SNMNYPTIAVSELKG ESKKI RTVTNVGG+G TVY VSVDA GE+DV+VIPEKLEF+KN
Sbjct: 669 SNMNYPTIAVSELKGKESKKISRTVTNVGGNGETVYTVSVDAAGELDVRVIPEKLEFTKN 728

Query: 728 NQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKSED 761
           NQKQSY+VVFTSTV TLKK+ FGSITW+NGK+RVRSPFVVTS+SS+ ED
Sbjct: 729 NQKQSYQVVFTSTVGTLKKDVFGSITWTNGKYRVRSPFVVTSKSSELED 775

BLAST of Carg08662 vs. ExPASy TrEMBL
Match: A0A5D3E6D0 (CO(2)-response secreted protease isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007880 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.5e-264
Identity = 475/562 (84.52%), Postives = 509/562 (90.57%), Query Frame = 0

Query: 1   MQTISSCVFL-LVCVIFLLLSENAKIA-AAAAGNNGVYIVYMGSA--SRTDFLRLLTSVN 60
           MQ+IS CVFL LVCV F L+SEN ++A AA    NGVYIVYMGSA  SR DFLRLL SVN
Sbjct: 1   MQSISFCVFLALVCVTFCLVSENVEVADAAEDAKNGVYIVYMGSASGSRIDFLRLLNSVN 60

Query: 61  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 120
           RR  NA+V TYKHGFTGFAARLSE EA AMRQ PGVVSVFPDPILKLHTTHSWDFLVSQT
Sbjct: 61  RR--NALVHTYKHGFTGFAARLSEHEAQAMRQSPGVVSVFPDPILKLHTTHSWDFLVSQT 120

Query: 121 SVKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 180
           SVK+D+ PK+D PTSSSQP DTIIGILDTGIWPESESF D+GM PIPSRWKGTCM GDDF
Sbjct: 121 SVKVDANPKSDSPTSSSQPYDTIIGILDTGIWPESESFNDKGMGPIPSRWKGTCMIGDDF 180

Query: 181 TSSNCNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCNRKI+GARFYESSESDGIR+HSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIA 300
           KGGSP SRIAMYRVCMADGC GSSIL AFDD+I DGVDVLSLSLG+P++ +P+LT DPIA
Sbjct: 241 KGGSPRSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGSPSFLRPDLTADPIA 300

Query: 301 IGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360
           IGAFHAVEKGITVVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGG 420
           EGINFS L+ SPVYPLI GKSA K+S SEDSARICSE SMDEA VKGKIVICESS EGGG
Sbjct: 361 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKGKIVICESSVEGGG 420

Query: 421 SYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVAT 480
           S WQ QAETV+SLGGVG+VLIDD +KLVAEKFT+P+T IS KDG E+L+YV+SSR PVAT
Sbjct: 421 SDWQSQAETVKSLGGVGVVLIDDDSKLVAEKFTTPMTVISKKDGPEILSYVNSSRKPVAT 480

Query: 481 ILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540
           ILPT T+INYKPAPA+ YFSSRGPNPAVLN+IKPDISAPGVNILAAWLGND++STPQ   
Sbjct: 481 ILPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQATK 540

Query: 541 SPLFNVISGTSMSCPHVSGVVA 559
           SPLFNVISGTSMSCPHVSGVVA
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVA 560

BLAST of Carg08662 vs. TAIR 10
Match: AT1G20160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 842.0 bits (2174), Expect = 3.7e-244
Identity = 437/766 (57.05%), Postives = 570/766 (74.41%), Query Frame = 0

Query: 11  LVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLR----LLTSVNRRNGNAVVRT 70
           L+C++F+  +E     A +   +GVYIVYMGSAS          L+ ++ +R  N ++ T
Sbjct: 16  LLCILFMTETE-----AGSRNGDGVYIVYMGSASSAANANRAQILINTMFKRRANDLLHT 75

Query: 71  YKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDSAPKA 130
           YKHGF+GFAARL+ +EA  + ++PGVVSVFPDP  +LHTTHSWDFL  QTSVK+DS P  
Sbjct: 76  YKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDSGP-- 135

Query: 131 DPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFTSSNCNRKIV 190
            P ++S    D+I+GILDTGIWPESESF D+ M PIPSRWKGTCM   DF SSNCNRKI+
Sbjct: 136 -PSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCNRKII 195

Query: 191 GARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIA 250
           GAR+Y++ + D   +++ RD  GHG+HV+ST AGSAV NASYYG+A+GTAKGGS  +RIA
Sbjct: 196 GARYYKNPDDDS-EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIA 255

Query: 251 MYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIAIGAFHAVEKG 310
           MY+VC   GC GSSILAAFDDAI DGVDVLSLSLGAP Y + +L TDPIAIGAFHAVE+G
Sbjct: 256 MYKVCNPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQG 315

Query: 311 ITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINFSALES 370
           I V+CSAGNDGP  G+V N APWI+TVAA+TIDRDFESDVVLG  KVIKGEGI+FS +  
Sbjct: 316 ILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFSNVSK 375

Query: 371 SPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGGSYWQGQA-ET 430
           SPVYPLIHGKSA  +  SE SAR C   S+D+  VKGKIV+CE+    GGSY+   A + 
Sbjct: 376 SPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCENV---GGSYYASSARDE 435

Query: 431 VESLGGVGIVLIDDQTKLVAEKFTS-PITAISSKDGDEVLAYVHSSRNPVATILPTLTVI 490
           V+S GG G V +DD+T+ VA  + S P T I SK+  E+ +Y++S+++PVATILPT TV 
Sbjct: 436 VKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPTATVE 495

Query: 491 NYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPENSPLFNVIS 550
            + PAPA+AYFSSRGP+    +++KPDI+APGV+ILAAW GND++ + + + +  +NVIS
Sbjct: 496 KFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWTGNDSSISLEGKPASQYNVIS 555

Query: 551 GTSMSCPHVSGVVAM-QFSHENF-----------TAIQTNNLESPMTLDTGSVATPYDYG 610
           GTSM+ PHVS V ++ +  H  +           TA QTNN +  +T +TG+ ATPYD G
Sbjct: 556 GTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTTATQTNNDKGLITTETGATATPYDSG 615

Query: 611 AGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPKDSNADYIS 670
           AGE+S+T +++PGLVYET+ TDYL +LC  GYN +TIK+++   P+ F CP DSN D IS
Sbjct: 616 AGELSSTASMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFPENFTCPADSNLDLIS 675

Query: 671 NMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPEKLEFSKNN 730
            +NYP+I +S  KG+ SK + RTVTNVG DG  VY VSV+ P   +++V PEKL+F+K+ 
Sbjct: 676 TINYPSIGISGFKGNGSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEKLQFTKDG 735

Query: 731 QKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTSESSKS 759
           +K +Y+V+ ++T S LK++ FG++TWSN K++VRSP V++SESS++
Sbjct: 736 EKLTYQVIVSATAS-LKQDVFGALTWSNAKYKVRSPIVISSESSRT 768

BLAST of Carg08662 vs. TAIR 10
Match: AT1G20160.2 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 825.5 bits (2131), Expect = 3.6e-239
Identity = 426/737 (57.80%), Postives = 552/737 (74.90%), Query Frame = 0

Query: 40  MGSASRTDFLR----LLTSVNRRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSV 99
           MGSAS          L+ ++ +R  N ++ TYKHGF+GFAARL+ +EA  + ++PGVVSV
Sbjct: 1   MGSASSAANANRAQILINTMFKRRANDLLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSV 60

Query: 100 FPDPILKLHTTHSWDFLVSQTSVKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFR 159
           FPDP  +LHTTHSWDFL  QTSVK+DS P   P ++S    D+I+GILDTGIWPESESF 
Sbjct: 61  FPDPHFQLHTTHSWDFLKYQTSVKVDSGP---PSSASDGSYDSIVGILDTGIWPESESFN 120

Query: 160 DEGMPPIPSRWKGTCMAGDDFTSSNCNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVA 219
           D+ M PIPSRWKGTCM   DF SSNCNRKI+GAR+Y++ + D   +++ RD  GHG+HV+
Sbjct: 121 DKDMGPIPSRWKGTCMEAKDFKSSNCNRKIIGARYYKNPDDDS-EYYTTRDVIGHGSHVS 180

Query: 220 STAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDV 279
           ST AGSAV NASYYG+A+GTAKGGS  +RIAMY+VC   GC GSSILAAFDDAI DGVDV
Sbjct: 181 STIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSSILAAFDDAIADGVDV 240

Query: 280 LSLSLGAPTYYKPELTTDPIAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAA 339
           LSLSLGAP Y + +L TDPIAIGAFHAVE+GI V+CSAGNDGP  G+V N APWI+TVAA
Sbjct: 241 LSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAA 300

Query: 340 STIDRDFESDVVLGNKKVIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGS 399
           +TIDRDFESDVVLG  KVIKGEGI+FS +  SPVYPLIHGKSA  +  SE SAR C   S
Sbjct: 301 NTIDRDFESDVVLGGNKVIKGEGIHFSNVSKSPVYPLIHGKSAKSADASEGSARACDSDS 360

Query: 400 MDEALVKGKIVICESSEEGGGSYWQGQA-ETVESLGGVGIVLIDDQTKLVAEKFTS-PIT 459
           +D+  VKGKIV+CE+    GGSY+   A + V+S GG G V +DD+T+ VA  + S P T
Sbjct: 361 LDQEKVKGKIVLCENV---GGSYYASSARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTT 420

Query: 460 AISSKDGDEVLAYVHSSRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDIS 519
            I SK+  E+ +Y++S+++PVATILPT TV  + PAPA+AYFSSRGP+    +++KPDI+
Sbjct: 421 VIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVAYFSSRGPSSLTRSILKPDIT 480

Query: 520 APGVNILAAWLGNDTNSTPQPENSPLFNVISGTSMSCPHVSGVVAM-QFSHENF------ 579
           APGV+ILAAW GND++ + + + +  +NVISGTSM+ PHVS V ++ +  H  +      
Sbjct: 481 APGVSILAAWTGNDSSISLEGKPASQYNVISGTSMAAPHVSAVASLIKSQHPTWGPSAIR 540

Query: 580 -----TAIQTNNLESPMTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCG 639
                TA QTNN +  +T +TG+ ATPYD GAGE+S+T +++PGLVYET+ TDYL +LC 
Sbjct: 541 SAIMTTATQTNNDKGLITTETGATATPYDSGAGELSSTASMQPGLVYETTETDYLNFLCY 600

Query: 640 RGYNRSTIKSIANTVPDGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGG 699
            GYN +TIK+++   P+ F CP DSN D IS +NYP+I +S  KG+ SK + RTVTNVG 
Sbjct: 601 YGYNVTTIKAMSKAFPENFTCPADSNLDLISTINYPSIGISGFKGNGSKTVTRTVTNVGE 660

Query: 700 DGATVYKVSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNG 759
           DG  VY VSV+ P   +++V PEKL+F+K+ +K +Y+V+ ++T S LK++ FG++TWSN 
Sbjct: 661 DGEAVYTVSVETPPGFNIQVTPEKLQFTKDGEKLTYQVIVSATAS-LKQDVFGALTWSNA 720

BLAST of Carg08662 vs. TAIR 10
Match: AT1G20150.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 714.9 bits (1844), Expect = 6.8e-206
Identity = 387/787 (49.17%), Postives = 526/787 (66.84%), Query Frame = 0

Query: 3   TISSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASR-----TDFLRLLTSVNR 62
           TI   +F  + VI    SE +K        +G YI+YMG+AS       D + LL+S+ +
Sbjct: 8   TIMFFMFFFLSVIQKCKSETSK--------SGDYIIYMGAASSDGSTDNDHVELLSSLLQ 67

Query: 63  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 122
           R+G   +  YKHGF+GFAA LSE EAH + ++PGV+SVFPD +L+LHTT SWDFLV ++ 
Sbjct: 68  RSGKTPMHRYKHGFSGFAAHLSEDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLVQESY 127

Query: 123 VKIDSAPKAD-PPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 182
            +     + +    S     DTIIG LD+GIWPE++SF D  M P+P +WKGTCM G   
Sbjct: 128 QRDTYFTEMNYEQESEMHEGDTIIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGKKT 187

Query: 183 --TSSNCNRKIVGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAG 242
              S  CNRK++GAR+Y SS      + +PRD  GHGTHVAS AAG  +ANASYYGLA+G
Sbjct: 188 QPDSFRCNRKLIGARYYNSSFFLDPDYETPRDFLGHGTHVASIAAGQIIANASYYGLASG 247

Query: 243 TAKGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDP 302
             +GGSP SRIAMYR C   GC GSSILAAFDDAI DGVDV+S+S+G    +   L  DP
Sbjct: 248 IMRGGSPSSRIAMYRACSLLGCRGSSILAAFDDAIADGVDVISISMG---LWPDNLLEDP 307

Query: 303 IAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLG--NKK 362
           ++IG+FHAVE+GITVVCS GN GP+  SV N APW++TVAASTIDR FES+++LG    +
Sbjct: 308 LSIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENR 367

Query: 363 VIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSE 422
           +I+G GIN + ++ +  YPLIH +SA K   +E++AR C+  ++D+ +VKGKIV+C+S  
Sbjct: 368 LIEGFGINIANIDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDSDL 427

Query: 423 EGGGSYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSP---ITAISSKDGDEVLAYVHS 482
           +     W  +++ V+ LGG+G+VL+DD++  ++  F  P   +T I  +DG ++++Y++S
Sbjct: 428 DNQVIQW--KSDEVKRLGGIGMVLVDDESMDLS--FIDPSFLVTIIKPEDGIQIMSYINS 487

Query: 483 SRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTN 542
           +R P+ATI+PT +   +  AP++  FSSRGP     +++KPDI+APGVNILA+WL  D N
Sbjct: 488 TREPIATIMPTRSRTGHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWLVGDRN 547

Query: 543 STPQPENSPLFNVISGTSMSCPHVSGVVAMQFSHE------------NFTAIQTNNLESP 602
           + P+ +  PLFN+ SGTSMSCPHVSG+ A   S                TA+Q  N  S 
Sbjct: 548 AAPEGKPPPLFNIESGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQMTNTGSH 607

Query: 603 MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVP 662
           +T +TG  ATPYD+GAG+++  G   PGL+YET+  DYL +L   G+    IK I+N +P
Sbjct: 608 ITTETGEKATPYDFGAGQVTIFGPSSPGLIYETNHMDYLNFLGYYGFTSDQIKKISNRIP 667

Query: 663 DGFDCPKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVG----GDGATVYKVSVDA 722
            GF CP+ SN   ISN+NYP+I++S   G ES+++ RTVTNV     GD  TVY VS+DA
Sbjct: 668 QGFACPEQSNRGDISNINYPSISISNFNGKESRRVSRTVTNVASRLIGDEDTVYTVSIDA 727

Query: 723 PGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTS 761
           P  + V+VIP +L F K   K SY+V+F+ST + LK +AFGSITWSNG + VRSPFVVTS
Sbjct: 728 PEGLLVRVIPRRLHFRKIGDKLSYQVIFSSTTTILKDDAFGSITWSNGMYNVRSPFVVTS 779

BLAST of Carg08662 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 498.4 bits (1282), Expect = 1.0e-140
Identity = 313/784 (39.92%), Postives = 443/784 (56.51%), Query Frame = 0

Query: 14  VIFLLLSENAKIAAAAAGNNGVYIVYMGSASRT----------------DFLRLLTSVNR 73
           ++ LLL   +     A+ ++  Y+VY G+ S                  DFL   T    
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 74  RNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQTS 133
           R  +A+  +Y     GFAA L    A+ + + P VVSVFP+  LKLHTT SWDFL  + +
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 134 VKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDFT 193
             +   P +     +    DTII  LDTG+WPES+SFRDEG+ PIPSRWKG C    D T
Sbjct: 130 SYV---PSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 189

Query: 194 SSNCNRKIVGARFYESSESDGI-----RFHSPRDGAGHGTHVASTAAGSAVANASYYGLA 253
             +CNRK++GAR++    +  +      F SPRD  GHG+H  STAAG  V   S +G  
Sbjct: 190 -FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQG 249

Query: 254 AGTAKGGSPGSRIAMYRVC----MADGCHGSSILAAFDDAINDGVDVLSLSLGA-PTYYK 313
            GTAKGGSP +R+A Y+VC      + C+ + +LAAFD AI+DG DV+S+SLG  PT + 
Sbjct: 250 NGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSF- 309

Query: 314 PELTTDPIAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVV 373
                D +AIG+FHA +K I VVCSAGN GP   +V N APW +TV AST+DR+F S++V
Sbjct: 310 ---FNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLV 369

Query: 374 LGNKKVIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVI 433
           LGN K  KG+ ++ +AL  +  YP++   +A   + S   A++C  GS+D    KGKI++
Sbjct: 370 LGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILV 429

Query: 434 CESSEEGGGSYWQGQAETVESLGGVGIVLIDDQ---TKLVAEKFTSPITAISSKDGDEVL 493
           C   + G       +   V   GG+G+VL +       L+A+    P T ++SKD   V 
Sbjct: 430 CLRGQNGRVE----KGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVS 489

Query: 494 AYVHSSRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWL 553
            Y+  ++ P+A I P+ T +  KPAP MA FSS+GP+     ++KPDI+APGV+++AA+ 
Sbjct: 490 RYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYT 549

Query: 554 G--NDTNSTPQPENSPLFNVISGTSMSCPHVSGVVAM---QFSHENFTAIQT-------- 613
           G  + TN    P    LFN ISGTSMSCPH+SG+  +   ++   +  AI++        
Sbjct: 550 GAVSPTNEQFDPRRL-LFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATI 609

Query: 614 -NNLESPMTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIK 673
            +++  P+   T   ATP+ +GAG +    A+ PGLVY+    DYL +LC  GYN S I 
Sbjct: 610 MDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQIS 669

Query: 674 SIANTVPDGFDC--PKDSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYK 733
             +    + F C  PK S    + N+NYP+I V  L  S+   + RTV NVG    ++Y 
Sbjct: 670 VFSG---NNFTCSSPKIS----LVNLNYPSITVPNLTSSK-VTVSRTVKNVG--RPSMYT 729

Query: 734 VSVDAPGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLKK-EAFGSITWSNGKHRVRS 752
           V V+ P  V V V P  L F+K  +++++KV+   +   + K   FG + WS+ KHRVRS
Sbjct: 730 VKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRS 770

BLAST of Carg08662 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 491.5 bits (1264), Expect = 1.2e-138
Identity = 316/784 (40.31%), Postives = 449/784 (57.27%), Query Frame = 0

Query: 5   SSCVFLLVCVIFLLLSENAKIAAAAAGNNGVYIVYMGSASRTDFLRL--------LTSVN 64
           S+  FLL+C+ F  +S       +++ + G YIV+M  +       L        L S++
Sbjct: 8   STAFFLLLCLGFCHVS-------SSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 67

Query: 65  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 124
             +   ++ TY++   GF+ RL+++EA ++  +PGV+SV P+   +LHTT +  FL    
Sbjct: 68  --DSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE 127

Query: 125 SVKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 184
                    AD    +   SD ++G+LDTG+WPES+S+ DEG  PIPS WKG C AG +F
Sbjct: 128 HT-------ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNF 187

Query: 185 TSSNCNRKIVGARF----YESSE---SDGIRFHSPRDGAGHGTHVASTAAGSAVANASYY 244
           T+S CNRK++GARF    YES+     +     SPRD  GHGTH +STAAGS V  AS  
Sbjct: 188 TASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 247

Query: 245 GLAAGTAKGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGA--PTYYK 304
           G A+GTA+G +P +R+A+Y+VC   GC  S ILAA D AI D V+VLS+SLG     YY+
Sbjct: 248 GYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 307

Query: 305 PELTTDPIAIGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVV 364
                D +AIGAF A+E+GI V CSAGN GP+  S+ N APWI TV A T+DRDF +  +
Sbjct: 308 -----DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 367

Query: 365 LGNKKVIKGEGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVI 424
           LGN K   G  +         + P I+  +A+ ++    +  +C  G++    VKGKIV+
Sbjct: 368 LGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT----NGNLCMTGTLIPEKVKGKIVM 427

Query: 425 CESSEEGGGSYWQGQAETVESLGGVGIVLID---DQTKLVAEKFTSPITAISSKDGDEVL 484
           C+     G +    + + V++ GGVG++L +   +  +LVA+    P T +  K GD + 
Sbjct: 428 CDR----GINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 487

Query: 485 AYVHSSRNPVATILPTLTVINYKPAPAMAYFSSRGPNPAVLNMIKPDISAPGVNILAAWL 544
            YV +  NP A+I    TV+  KP+P +A FSSRGPN    N++KPD+ APGVNILAAW 
Sbjct: 488 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 547

Query: 545 G-NDTNSTPQPENSPLFNVISGTSMSCPHVSGVVAMQFS-HENF-----------TAIQT 604
           G               FN+ISGTSMSCPHVSG+ A+  S H  +           TA +T
Sbjct: 548 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 607

Query: 605 NNLESP-MTLDTGSVATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIK 664
                P + + TG  +TP+D+GAG +S T A  PGL+Y+ +T DYL +LC   Y    I+
Sbjct: 608 YKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR 667

Query: 665 SIANTVPDGFDCPKDSNADY-ISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKV 724
           S++      + C  D +  Y ++++NYP+ AV+ + G  + K  RTVT+VGG G    KV
Sbjct: 668 SVSRR---NYTC--DPSKSYSVADLNYPSFAVN-VDGVGAYKYTRTVTSVGGAGTYSVKV 727

Query: 725 SVDAPGEVDVKVIPEKLEFSKNNQKQSYKVVFTSTVSTLK-KEAFGSITWSNGKHRVRSP 753
           + +  G V + V P  L F + N+K+SY V FT   S      +FGSI WS+GKH V SP
Sbjct: 728 TSETTG-VKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSP 755

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024139.10.0e+00100.00CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. argyros... [more]
KAG6591258.10.0e+0097.41CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022937005.10.0e+0097.15CO(2)-response secreted protease [Cucurbita moschata][more]
XP_022975820.10.0e+0096.11CO(2)-response secreted protease [Cucurbita maxima][more]
XP_023535470.10.0e+0095.98CO(2)-response secreted protease [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LNU15.2e-24357.05CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV... [more]
F4HSQ29.5e-20549.17Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 S... [more]
A9JQS75.5e-15242.76Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1[more]
Q9ZSP51.4e-13939.92Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
O653511.7e-13740.31Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1FFB50.0e+0097.15CO(2)-response secreted protease OS=Cucurbita moschata OX=3662 GN=LOC111443427 P... [more]
A0A6J1IHT10.0e+0096.11CO(2)-response secreted protease OS=Cucurbita maxima OX=3661 GN=LOC111476411 PE=... [more]
A0A0A0LJ710.0e+0081.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873800 PE=3 SV=1[more]
A0A6J1C9380.0e+0080.36CO(2)-response secreted protease OS=Momordica charantia OX=3673 GN=LOC111009346 ... [more]
A0A5D3E6D01.5e-26484.52CO(2)-response secreted protease isoform X2 OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT1G20160.13.7e-24457.05Subtilisin-like serine endopeptidase family protein [more]
AT1G20160.23.6e-23957.80Subtilisin-like serine endopeptidase family protein [more]
AT1G20150.16.8e-20649.17Subtilisin-like serine endopeptidase family protein [more]
AT2G04160.11.0e-14039.92Subtilisin-like serine endopeptidase family protein [more]
AT5G67360.11.2e-13840.31Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 135..154
score: 26.89
coord: 544..560
score: 50.94
coord: 206..219
score: 53.53
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 136..562
e-value: 2.7E-40
score: 138.4
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 137..615
e-value: 1.4E-160
score: 537.1
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 133..606
NoneNo IPR availableGENE3D2.60.40.2310coord: 619..754
e-value: 6.7E-37
score: 128.1
NoneNo IPR availableGENE3D3.50.30.30coord: 339..484
e-value: 1.4E-160
score: 537.1
NoneNo IPR availablePANTHERPTHR10795:SF647SUBTILISIN-LIKE PROTEASE SBT5.1coord: 41..753
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 108..602
score: 24.229315
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 345..477
e-value: 9.74474E-24
score: 95.1731
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 35..102
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 653..751
e-value: 1.2E-28
score: 99.2
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 20..104
e-value: 8.7E-18
score: 66.6
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 35..104
e-value: 5.5E-12
score: 46.2
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 41..753
IPR022398Peptidase S8, subtilisin, His-active sitePROSITEPS00137SUBTILASE_HIScoord: 210..220
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 545..555
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 101..559
e-value: 3.42955E-118
score: 356.138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08662-RACarg08662-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity