Carg08653 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08653
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMechanosensitive ion channel protein
LocationCarg_Chr09: 347667 .. 353882 (-)
RNA-Seq ExpressionCarg08653
SyntenyCarg08653
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCCAAAGGAAGATGTTATCCTCAAGGTCAACAGTTTTGGAAATGGCGACAAGGAATTAGTGGAGAAGAAGTTCCAGTGTTTACCAGAGAGATCCTTGCAAACCATGAGACGAAGAGGTAAAGAATTTTCCGAGCCGGAAAACCGCCTGCCGTCCTCGGGCTCCAGCGGCGGCGAGGTTCTCCGTTGCAGTTCCAACTATTCGTTTTCTGGAAGTTCATGGAGCTCACCGATTAGCAAGAATTCTCGGTTGATGGATCCTCCTGAGGATGATTCTTCTCAAAAGCTGGAAACCGCCGTGAAAGATGAGGGTAACAACAACGATAATAAAGACGCCACCGAGGATATGCCTGAAGAGTACAATCAACTGAAGTTAAGTCCCTTATTAGGTTTCCAGTTGGTAGTTTTTGTTCTCATTATTGGGAGTTTGATTTGTAATCGATGGATTTCAATCTTAAAGAGGGAGACGTTTTGGGGTCTTCCGCTGTGGAAATGGGAGTTGACTGTTCTAGCGGTAATCTGTGGACATATGGTTTCATCTATGGCCGTTGGATTGATGGTGAAAATCATCGAGATGAATTTCCTGTTAAGGAAACGGGTTCTGTATTTCGTTTATGGATTGAGAAAGGCTGTAAAAAATTGCCTGTGGATGTCTCTGATTTTGCTTGTTTGGCATTTCATCTTCAACGACAAGGTTCAGAGAAAGACGCAGAGTAAGATCTTGCCTTATGTCACCAAGATTTTGATCTGTTTTCTCGTGGCTGCTTCGATATGGCTTCTGAAAACGCTTGTCATTAAGATTCTGGCTTCGTTTTTCCACGTTAATAACTACTTCGAAAGGCTTAAGGAGGCTTTGTTCAGTCAGTACGTGATCGTGACGCTCTCTGGTTTGCCATTGTTTGAGAGGCCAAATTCTGAAGATATGATGAAGGATGAACAAATTGCCGATGGAATAGCAGAAAGTTACAAAAGTGGCAGAGTTGTATGCAGTGGCAGACTCGTAAATTGCAATGGCTCGAGACAATTGAAGGTAGAATCAAAAAGAAAAGATGAGGAAATCCCAGTTGATGAGTTAAATAAACTGAACCAGAAGAATATATCAGCTTGGAATATGAGGAGAATGATGAATATTGTAAGAAATGGAGCTTTGTTAACTCTTGATGAGAAGATACTGTCGGAGACGGAAGAACAATCTTTGTTGCAGATTAGGAATGAACGGCAGGCGAAAGAGGCAGCTGGGAGGATTTTCCGGCGAGTGGCCCAACGTGGGTCAGAGTGAGTTCTCTTTTTGAATTCGTTGTTTCTTTCATGAATATTCAATTTCATTGCATGATGTGAGCTATGTTTGTTGCAGCTGTATTTATAAAGAAGATGTAATGCGATTTATGAATAAAGAGGAAGCTTTAGTGACGATGAGTTTGTTTGGAACAACCGCTGAAACTCAATGCATTGATGAGTCCTCTTTTAGAGAGTGGATGGTAAGGCCCTGCCCTTGACTCTACTTGCCTTGTTCTGTTTTGCTGTGTAATGAAGATGATATATTTGGAGGTTGATGTTTTAGTTAAAGCTTATAAGACGTTTCTATCTATGTCAAATTTATGAAACTCCAAATGTCCCTACTCAAAATGCATGGTACCTTACCCTCCTACCCCTATATGTTTTTCAATGAGAAATAGTCCTAAAGTCAACCTTAAAACCCGACCCATTTCGTTAGCTTTAGAAGAGAAAGCCGCTGGAGAAAAATGTCTTTATGAATACTAAACTCACTTTCTCACTCTCACGTCACTATACTCACATGCGGTGAGATCCTACATTTGTTGGAGAGGGGAACGAAACATTCGTTATAAGGGTGTGGAAACCTGAGTGGATGCGTTTTAAGACCTTGATTGGGAGTCTGGAAGGGAAAGCCTAAAGATGATAATATCTGTTAGCTGTGCACTTGGGTGTGAAACTGTGAGATCCCACATCAATTGAAGAGGGGAATAAGTGTCAGCGAGGACGCTGGGCCCGAAGGGAGGTGGATTGTGAGATCCCGCATCGGTTGGAAAGGGGAACTAAGCATTCTTTGTAGGGGTGTGGAAACCTCTCCTAGCGGACGCGTTTCAAAGACCTTGAGAGGAAACCCGAGAGGGAAAGCCCAAAAGAGGACAATATCTGCTAACGGTGGGCTTGGATTGTTATAAATGGTATCAAAACCAGACACCGGGCGGTGTACCAGCAAAGACGTTGGGTCCCAAAGGGGGGAGGGGATTATGAGATCCCACATTGGTTGGAGCGGAGAACGAAGCATTCTTTGTAGGGATGTGTAAACCTCTCCCTATCAATCACGTTTTAAAAACCTTAAAGGGAAGCACGAAAGGGAAAAGCCCAAAACGATGCCGAAACCATTTAGATGTGTATATACTAAGAATTCTTAACCTAAACAGCAATGTAATGGATATATTTTATTTTTCTTTGATGTGTTTCAGGTTAATGCATTTAAGCAGAGAAAAGTGCTTGTTCTATCTCTCAATGATGCAAATACAGCTGTTGATGATCTTCACAACTTGCTAAACGTCTTTGTAGCTTTAACAATTTTGGTAATCTGGCTTATCATATTTGGAACTCGCGTCCTGCACTTCCTCATCTTCATAACTTCACAGCTTCTTCTTCTGGCTTTTATTTTTGGCAACACATGTAAGACGGTCTTTGAAGCTATCATTTTCTTGTTTGTGATGCACCCATTCGACGTTGGCGATCGCTGCGAAGTGGACGGCGTCGAGGTGCAAAACTCTCTAGCCAAACAATTTGTTTTGCTCTTAGTGACGCATTCTTAACTTTCTTCGGTTTTCCAGATGGTTGTTGAAGAGATGAAGATATTAACAACAGTTTTCCTGAGATATGATAATCAAAGGATCAGCTACCCCAACAGTGTGCTGGCTACCAAGGCCATTGGCAATTACTACCGTAGTCCAGACATGGGAGAGGGAGTTGACTTCTATATCCACATCTCTACTCCATGGGAGAAAGTTGCCCTTTTGAAAGAACGGATAACCAGGTATGCTTTAATTTGGAATAGAGTGTATTTATCGATTTGGCTCTCAGAATTTGTGAGGTCCCACAACCGTTGGAAAGGAGAACGAAGCATTCCTTATAAGGGTTATAAGGGTGTGGAAATATCTCCCTCCATAGTAGATGCATTTTAAAGGTTGACGGCGTTACAAATGGTATCAGAGCGGGGCAATGTGCCAGTGAGGACACTTGACTCCAAGGAGGTGGACTGTGAGGTCCCACATCGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCATAAGTGTTTTAAAGGCTGACAAAGACACGTAACGACCCAAAACTGATAATATATGTTAGCTGTGGATTTGGGCTAATATAAAATTCGAGAGAATGGAAAGTGTAATGGGTTTGATGAGTGAATGTATGCAGGTATGTTGAGAGCATGGGCGAGCAATGGCACCCAGATCCACAGATTGTGATGAAGGATGTTGAAGATCTTGAGAAGGTGAGGATGTCGGTGGGTCTTACGCACAAATTGAACCATCAGAACATGAAAGAGAGGTGGAACAGGAGAGCTGCGCTGGTTCAAGAGATTGTCACAATTTTAGACTCAACATGACCACAATCTACTCAATCTACTCTATATTGCCTTTCTTTCGGGACTTATTGTAAGTGTCATTTTACTTCAAAAGTCATTTCAAACCTAACTCTCATTTTAGCCATTTTTTAGCCATTTTGTACATTATTTGAACGTGAAATTGTAGACGTACGACTGTATTATGATAGAAGAAAAATTAGTTGAGTTTTTTTTTCTTGCAGATTGAATTGAACGTAGAAGGAAGGATTTTTTTTTTTCTTCAGCAAGTAAGCTTAAACTATTATAAATGGTATCTAACTAGTTAGGAAAATGGAAATTTGATTTAGAAAGTTTTGGATTTTTTTAATTTAAAATGTAAAATAAAATGATTTCAGATTTTTTTTTTTATTATTTTCAATTGAAACTAAATTATGATTGAAAAGTTAAATTCAATTATTTTTTATAAGAAAAAGTGAGAGTATATATATTACTTCTTTAAAATCCAAAGAAAACCCCTCTCTCACTCCTCTTTGTTATTTCCCTCCTCCACCCACGCAAACACACCTCCTCTTACCTCTGCCTCCAATTCCAGCACCCACATAGCCGAAGTTGGGGGCGGCGGTCCACGGCCACGGCGATTACTTCAGACGGCGGCGCAGCAGTTCCCGACCATACCCGGAAACTGAAGAACCGCGCGGCGGTACCCTCCAGCGGCAGTCACGCGCAGTTCCACCACCTCCGACGCTTCCTGTAGCAGATCCGGCGGCGCGAACGGTATCCAACCGAGATTTCTCTCCGATTCACACGTTCTGGCGATGAGTCAGTGGTGACCTAAAGGTCAGTCAGCTCTCATCGGCAGCCCTGCAGTTCCACATTTCGGCATTGACGGTGAGTTCGATTTCGGCAGGAACAGTCAGATTCGCCACCTCCATAACGCACAGGCACCTCCGACGAACAGCGCAGACTGACTCCTTCCCTCCGCTGTACTCCGACGGTGAACGATCCGGTCAATTTTCCCTTGTGAATTTAGGTGAGGTTTAACCTTTTAGTCTTTGATTGAACTTTCTAAGAAGTTTTTCGAAATTTGTTGTTTGGGATGTTAAAATTTTTGCTTATATAACCTTTGGAAATAGGATGGCATTCTTTTTTATTAAAAAAAAAAAATACATTGATTCAAATATTTGATGACCAATAACTCATCGTGATGATTGGAAGTTAAAATCTAATTTCTCGTATATGCTATCTGTGTCCATCAAATCATGATTTAAACATATCAAGTCAATAGTTTGTACTGATAGTTATATAGAAATATGTAACTGTGAAAATTAATTAGGCTTGCTGCAAAAGTTATAGATAATGCTGATTTGCTTAAGTGAATTTATGTTAAAGAGTGATAGAAATCATAACTGTGTAGCGATGAAGGAGGACGGAGATGTATCATTGAAGTCCAGCAGTAAAGAAGAGGAGAATGTCGTAGGTTACCACAGCACCAACGTGAAGAAAGTTAGGTTAAATTCAACTCTCACTGCTTTGCTTGACGATCCTATCCTGGCGGATGTCCCCAAGAAGCCCACCTTACTGGACGTTGATACGCTCATCAGCCTCGAATTGGGCAGCGCCATGCGCATCTCTGTCCTTAAGCTCGACGGCACCATAATTGGTACACACACTTTTCTAATGTTCCTCCTTTTTCTCTAACTGGCATTTTCACAAACACTTTGTTTATAGAACTGCTGAAATGCGATTTGCGATCTTGTTCATGCTTTAGATGTGGCGATCATGAATTCCGCCTTGTTGAAGGACTTGAAGCTCGCTATCAAGAAGAAAGTAAACGAGATGGAGCAATCCAAGATGGGACATCGCCACATTTCATGGTACTCTTTCTTCATTCTTGGTGAATTCAACCATAAAAAGTGATTAGTAGATAGCTTTCTTAGCTAATCGTACAATGATCTTTGTGCATTGATGAGAAGTCTTTCGCTTGTGGATCTGCAGGAAGCATGTGTGGGCAAATTTTTGCCTAGCACACCACAACGAGAAGATTCTTGATGATAGCTCTGCGCTTCAGGATTTTGGAATCCGTAATAATTCTCAGGTTATTTCCTAAACCTTGTATGATCCGATTGACATTCTAACCAGCAGAAATCATGTCAAAGTATTAGAAATTCTATCCAGGCTGAAAATTCAATCATAATATACAGAGAACAAAAACTTGTTGCAGGTGCATTTTGTCCCGTATGTCATGTCAAAGAATTCCAGAAGGCATTCTCGAAGGAGAAAACACCGTTTCTTTCACGGTCTCAACAAATGCACATGAACAGACACTTCGGGGTATGATTCTCAAACACAAATTCAGTAAGCTTGAGTTCGCACAACTTTTGTAACTGGTTTTGAACTGTGAAGTGAATGAGTTATAGAAGTTCGAGCTCGCATAAACATTATGATATGTAAAATTGTGTAGCTTGTTTCTAGACTTAAAATCCCAAATCCTCTACCTTGAACTCATGACCTAAACCATATGGCAGAGTTGAAACATTTATTCTCTACCCTCACTATCTATGTACTAAACTCATGCTTTTGCTTTAAACTAGTGGAGATATTTG

mRNA sequence

ATGGCCCCAAAGGAAGATGTTATCCTCAAGGTCAACAGTTTTGGAAATGGCGACAAGGAATTAGTGGAGAAGAAGTTCCAGTGTTTACCAGAGAGATCCTTGCAAACCATGAGACGAAGAGGTAAAGAATTTTCCGAGCCGGAAAACCGCCTGCCGTCCTCGGGCTCCAGCGGCGGCGAGGTTCTCCGTTGCAGTTCCAACTATTCGTTTTCTGGAAGTTCATGGAGCTCACCGATTAGCAAGAATTCTCGGTTGATGGATCCTCCTGAGGATGATTCTTCTCAAAAGCTGGAAACCGCCGTGAAAGATGAGGGTAACAACAACGATAATAAAGACGCCACCGAGGATATGCCTGAAGAGTACAATCAACTGAAGTTAAGTCCCTTATTAGGTTTCCAGTTGGTAGTTTTTGTTCTCATTATTGGGAGTTTGATTTGTAATCGATGGATTTCAATCTTAAAGAGGGAGACGTTTTGGGGTCTTCCGCTGTGGAAATGGGAGTTGACTGTTCTAGCGGTAATCTGTGGACATATGGTTTCATCTATGGCCGTTGGATTGATGGTGAAAATCATCGAGATGAATTTCCTGTTAAGGAAACGGGTTCTGTATTTCGTTTATGGATTGAGAAAGGCTGTAAAAAATTGCCTGTGGATGTCTCTGATTTTGCTTGTTTGGCATTTCATCTTCAACGACAAGGTTCAGAGAAAGACGCAGAGTAAGATCTTGCCTTATGTCACCAAGATTTTGATCTGTTTTCTCGTGGCTGCTTCGATATGGCTTCTGAAAACGCTTGTCATTAAGATTCTGGCTTCGTTTTTCCACGTTAATAACTACTTCGAAAGGCTTAAGGAGGCTTTGTTCAGTCAGTACGTGATCGTGACGCTCTCTGGTTTGCCATTGTTTGAGAGGCCAAATTCTGAAGATATGATGAAGGATGAACAAATTGCCGATGGAATAGCAGAAAGTTACAAAAGTGGCAGAGTTGTATGCAGTGGCAGACTCGTAAATTGCAATGGCTCGAGACAATTGAAGGTAGAATCAAAAAGAAAAGATGAGGAAATCCCAGTTGATGAGTTAAATAAACTGAACCAGAAGAATATATCAGCTTGGAATATGAGGAGAATGATGAATATTGTAAGAAATGGAGCTTTGTTAACTCTTGATGAGAAGATACTGTCGGAGACGGAAGAACAATCTTTGTTGCAGATTAGGAATGAACGGCAGGCGAAAGAGGCAGCTGGGAGGATTTTCCGGCGAGTGGCCCAACGTGGGTCAGACTGTATTTATAAAGAAGATGTAATGCGATTTATGAATAAAGAGGAAGCTTTAGTGACGATGAGTTTGTTTGGAACAACCGCTGAAACTCAATGCATTGATGAGTCCTCTTTTAGAGAGTGGATGGTTAATGCATTTAAGCAGAGAAAAGTGCTTGTTCTATCTCTCAATGATGCAAATACAGCTGTTGATGATCTTCACAACTTGCTAAACGTCTTTGTAGCTTTAACAATTTTGGTAATCTGGCTTATCATATTTGGAACTCGCGTCCTGCACTTCCTCATCTTCATAACTTCACAGCTTCTTCTTCTGGCTTTTATTTTTGGCAACACATGTAAGACGGTCTTTGAAGCTATCATTTTCTTGTTTGTGATGCACCCATTCGACGTTGGCGATCGCTGCGAAGTGGACGGCGTCGAGATGGTTGTTGAAGAGATGAAGATATTAACAACAGTTTTCCTGAGATATGATAATCAAAGGATCAGCTACCCCAACAGTGTGCTGGCTACCAAGGCCATTGGCAATTACTACCGTAGTCCAGACATGGGAGAGGGAGATGTTGAAGATCTTGAGAAGGTGAGGATGTCGGTGGGTCTTACGCACAAATTGAACCATCAGAACATGAAAGAGAGGTGGAACAGGAGAGCTGCGCTGATTGAATTGAACGTAGAAGGAAGGATTTTTTTTTTTCTTCAGCAACCGAAGTTGGGGGCGGCGGTCCACGGCCACGGCGATTACTTCAGACGGCGGCGCAGCAGTTCCCGACCATACCCGGAAACTGAAGAACCGCGCGGCGGTACCCTCCAGCGGCAGTCACGCGCAGTTCCACCACCTCCGACGCTTCCTGTAGCAGATCCGGCGGCGCGAACGGTCAGTCAGCTCTCATCGGCAGCCCTGCAGTTCCACATTTCGGCATTGACGGTGAGTTCGATTTCGGCAGGAACAGTCAGATTCGCCACCTCCATAACGCACAGGCACCTCCGACGAACAGCGCAGACTGACTCCTTCCCTCCGCTGTACTCCGACGGTGAACGATCCGGTCAATTTTCCCTTGTGAATTTAGCGATGAAGGAGGACGGAGATGTATCATTGAAGTCCAGCAGTAAAGAAGAGGAGAATGTCGTAGGTTACCACAGCACCAACGTGAAGAAAGTTAGGTTAAATTCAACTCTCACTGCTTTGCTTGACGATCCTATCCTGGCGGATGTCCCCAAGAAGCCCACCTTACTGGACGTTGATACGCTCATCAGCCTCGAATTGGGCAGCGCCATGCGCATCTCTGTCCTTAAGCTCGACGGCACCATAATTGATGTGGCGATCATGAATTCCGCCTTGTTGAAGGACTTGAAGCTCGCTATCAAGAAGAAAGTAAACGAGATGGAGCAATCCAAGATGGGACATCGCCACATTTCATGGAAGCATGTGTGGGCAAATTTTTGCCTAGCACACCACAACGAGAAGATTCTTGATGATAGCTCTGCGCTTCAGGATTTTGGAATCCGTGCATTTTGTCCCGTATGTCATGTCAAAGAATTCCAGAAGGCATTCTCGAAGGAGAAAACACCGTTTCTTTCACGGTCTCAACAAATGCACATGAACAGACACTTCGGGGTATGATTCTCAAACACAAATTCAGTAAGCTTGAGTTCGCACAACTTTTGTAACTGGTTTTGAACTGTGAAGTGAATGAGTTATAGAAGTTCGAGCTCGCATAAACATTATGATATGTAAAATTGTGTAGCTTGTTTCTAGACTTAAAATCCCAAATCCTCTACCTTGAACTCATGACCTAAACCATATGGCAGAGTTGAAACATTTATTCTCTACCCTCACTATCTATGTACTAAACTCATGCTTTTGCTTTAAACTAGTGGAGATATTTG

Coding sequence (CDS)

ATGGCCCCAAAGGAAGATGTTATCCTCAAGGTCAACAGTTTTGGAAATGGCGACAAGGAATTAGTGGAGAAGAAGTTCCAGTGTTTACCAGAGAGATCCTTGCAAACCATGAGACGAAGAGGTAAAGAATTTTCCGAGCCGGAAAACCGCCTGCCGTCCTCGGGCTCCAGCGGCGGCGAGGTTCTCCGTTGCAGTTCCAACTATTCGTTTTCTGGAAGTTCATGGAGCTCACCGATTAGCAAGAATTCTCGGTTGATGGATCCTCCTGAGGATGATTCTTCTCAAAAGCTGGAAACCGCCGTGAAAGATGAGGGTAACAACAACGATAATAAAGACGCCACCGAGGATATGCCTGAAGAGTACAATCAACTGAAGTTAAGTCCCTTATTAGGTTTCCAGTTGGTAGTTTTTGTTCTCATTATTGGGAGTTTGATTTGTAATCGATGGATTTCAATCTTAAAGAGGGAGACGTTTTGGGGTCTTCCGCTGTGGAAATGGGAGTTGACTGTTCTAGCGGTAATCTGTGGACATATGGTTTCATCTATGGCCGTTGGATTGATGGTGAAAATCATCGAGATGAATTTCCTGTTAAGGAAACGGGTTCTGTATTTCGTTTATGGATTGAGAAAGGCTGTAAAAAATTGCCTGTGGATGTCTCTGATTTTGCTTGTTTGGCATTTCATCTTCAACGACAAGGTTCAGAGAAAGACGCAGAGTAAGATCTTGCCTTATGTCACCAAGATTTTGATCTGTTTTCTCGTGGCTGCTTCGATATGGCTTCTGAAAACGCTTGTCATTAAGATTCTGGCTTCGTTTTTCCACGTTAATAACTACTTCGAAAGGCTTAAGGAGGCTTTGTTCAGTCAGTACGTGATCGTGACGCTCTCTGGTTTGCCATTGTTTGAGAGGCCAAATTCTGAAGATATGATGAAGGATGAACAAATTGCCGATGGAATAGCAGAAAGTTACAAAAGTGGCAGAGTTGTATGCAGTGGCAGACTCGTAAATTGCAATGGCTCGAGACAATTGAAGGTAGAATCAAAAAGAAAAGATGAGGAAATCCCAGTTGATGAGTTAAATAAACTGAACCAGAAGAATATATCAGCTTGGAATATGAGGAGAATGATGAATATTGTAAGAAATGGAGCTTTGTTAACTCTTGATGAGAAGATACTGTCGGAGACGGAAGAACAATCTTTGTTGCAGATTAGGAATGAACGGCAGGCGAAAGAGGCAGCTGGGAGGATTTTCCGGCGAGTGGCCCAACGTGGGTCAGACTGTATTTATAAAGAAGATGTAATGCGATTTATGAATAAAGAGGAAGCTTTAGTGACGATGAGTTTGTTTGGAACAACCGCTGAAACTCAATGCATTGATGAGTCCTCTTTTAGAGAGTGGATGGTTAATGCATTTAAGCAGAGAAAAGTGCTTGTTCTATCTCTCAATGATGCAAATACAGCTGTTGATGATCTTCACAACTTGCTAAACGTCTTTGTAGCTTTAACAATTTTGGTAATCTGGCTTATCATATTTGGAACTCGCGTCCTGCACTTCCTCATCTTCATAACTTCACAGCTTCTTCTTCTGGCTTTTATTTTTGGCAACACATGTAAGACGGTCTTTGAAGCTATCATTTTCTTGTTTGTGATGCACCCATTCGACGTTGGCGATCGCTGCGAAGTGGACGGCGTCGAGATGGTTGTTGAAGAGATGAAGATATTAACAACAGTTTTCCTGAGATATGATAATCAAAGGATCAGCTACCCCAACAGTGTGCTGGCTACCAAGGCCATTGGCAATTACTACCGTAGTCCAGACATGGGAGAGGGAGATGTTGAAGATCTTGAGAAGGTGAGGATGTCGGTGGGTCTTACGCACAAATTGAACCATCAGAACATGAAAGAGAGGTGGAACAGGAGAGCTGCGCTGATTGAATTGAACGTAGAAGGAAGGATTTTTTTTTTTCTTCAGCAACCGAAGTTGGGGGCGGCGGTCCACGGCCACGGCGATTACTTCAGACGGCGGCGCAGCAGTTCCCGACCATACCCGGAAACTGAAGAACCGCGCGGCGGTACCCTCCAGCGGCAGTCACGCGCAGTTCCACCACCTCCGACGCTTCCTGTAGCAGATCCGGCGGCGCGAACGGTCAGTCAGCTCTCATCGGCAGCCCTGCAGTTCCACATTTCGGCATTGACGGTGAGTTCGATTTCGGCAGGAACAGTCAGATTCGCCACCTCCATAACGCACAGGCACCTCCGACGAACAGCGCAGACTGACTCCTTCCCTCCGCTGTACTCCGACGGTGAACGATCCGGTCAATTTTCCCTTGTGAATTTAGCGATGAAGGAGGACGGAGATGTATCATTGAAGTCCAGCAGTAAAGAAGAGGAGAATGTCGTAGGTTACCACAGCACCAACGTGAAGAAAGTTAGGTTAAATTCAACTCTCACTGCTTTGCTTGACGATCCTATCCTGGCGGATGTCCCCAAGAAGCCCACCTTACTGGACGTTGATACGCTCATCAGCCTCGAATTGGGCAGCGCCATGCGCATCTCTGTCCTTAAGCTCGACGGCACCATAATTGATGTGGCGATCATGAATTCCGCCTTGTTGAAGGACTTGAAGCTCGCTATCAAGAAGAAAGTAAACGAGATGGAGCAATCCAAGATGGGACATCGCCACATTTCATGGAAGCATGTGTGGGCAAATTTTTGCCTAGCACACCACAACGAGAAGATTCTTGATGATAGCTCTGCGCTTCAGGATTTTGGAATCCGTGCATTTTGTCCCGTATGTCATGTCAAAGAATTCCAGAAGGCATTCTCGAAGGAGAAAACACCGTTTCTTTCACGGTCTCAACAAATGCACATGAACAGACACTTCGGGGTATGA

Protein sequence

MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGEVLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPLFERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRVAQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYYRSPDMGEGDVEDLEKVRMSVGLTHKLNHQNMKERWNRRAALIELNVEGRIFFFLQQPKLGAAVHGHGDYFRRRRSSSRPYPETEEPRGGTLQRQSRAVPPPPTLPVADPAARTVSQLSSAALQFHISALTVSSISAGTVRFATSITHRHLRRTAQTDSFPPLYSDGERSGQFSLVNLAMKEDGDVSLKSSSKEEENVVGYHSTNVKKVRLNSTLTALLDDPILADVPKKPTLLDVDTLISLELGSAMRISVLKLDGTIIDVAIMNSALLKDLKLAIKKKVNEMEQSKMGHRHISWKHVWANFCLAHHNEKILDDSSALQDFGIRAFCPVCHVKEFQKAFSKEKTPFLSRSQQMHMNRHFGV
Homology
BLAST of Carg08653 vs. NCBI nr
Match: KAG7024130.1 (Mechanosensitive ion channel protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1857.4 bits (4810), Expect = 0.0e+00
Identity = 963/963 (100.00%), Postives = 963/963 (100.00%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60
           MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE
Sbjct: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60

Query: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120
           VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120

Query: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180
           YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS
Sbjct: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180

Query: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240
           SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK
Sbjct: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240

Query: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300
           ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL
Sbjct: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300

Query: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360
           FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN
Sbjct: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360

Query: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420
           KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV
Sbjct: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420

Query: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480
           AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS
Sbjct: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480

Query: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540
           LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV
Sbjct: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540

Query: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600
           FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN
Sbjct: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600

Query: 601 YYRSPDMGEGDVEDLEKVRMSVGLTHKLNHQNMKERWNRRAALIELNVEGRIFFFLQQPK 660
           YYRSPDMGEGDVEDLEKVRMSVGLTHKLNHQNMKERWNRRAALIELNVEGRIFFFLQQPK
Sbjct: 601 YYRSPDMGEGDVEDLEKVRMSVGLTHKLNHQNMKERWNRRAALIELNVEGRIFFFLQQPK 660

Query: 661 LGAAVHGHGDYFRRRRSSSRPYPETEEPRGGTLQRQSRAVPPPPTLPVADPAARTVSQLS 720
           LGAAVHGHGDYFRRRRSSSRPYPETEEPRGGTLQRQSRAVPPPPTLPVADPAARTVSQLS
Sbjct: 661 LGAAVHGHGDYFRRRRSSSRPYPETEEPRGGTLQRQSRAVPPPPTLPVADPAARTVSQLS 720

Query: 721 SAALQFHISALTVSSISAGTVRFATSITHRHLRRTAQTDSFPPLYSDGERSGQFSLVNLA 780
           SAALQFHISALTVSSISAGTVRFATSITHRHLRRTAQTDSFPPLYSDGERSGQFSLVNLA
Sbjct: 721 SAALQFHISALTVSSISAGTVRFATSITHRHLRRTAQTDSFPPLYSDGERSGQFSLVNLA 780

Query: 781 MKEDGDVSLKSSSKEEENVVGYHSTNVKKVRLNSTLTALLDDPILADVPKKPTLLDVDTL 840
           MKEDGDVSLKSSSKEEENVVGYHSTNVKKVRLNSTLTALLDDPILADVPKKPTLLDVDTL
Sbjct: 781 MKEDGDVSLKSSSKEEENVVGYHSTNVKKVRLNSTLTALLDDPILADVPKKPTLLDVDTL 840

Query: 841 ISLELGSAMRISVLKLDGTIIDVAIMNSALLKDLKLAIKKKVNEMEQSKMGHRHISWKHV 900
           ISLELGSAMRISVLKLDGTIIDVAIMNSALLKDLKLAIKKKVNEMEQSKMGHRHISWKHV
Sbjct: 841 ISLELGSAMRISVLKLDGTIIDVAIMNSALLKDLKLAIKKKVNEMEQSKMGHRHISWKHV 900

Query: 901 WANFCLAHHNEKILDDSSALQDFGIRAFCPVCHVKEFQKAFSKEKTPFLSRSQQMHMNRH 960
           WANFCLAHHNEKILDDSSALQDFGIRAFCPVCHVKEFQKAFSKEKTPFLSRSQQMHMNRH
Sbjct: 901 WANFCLAHHNEKILDDSSALQDFGIRAFCPVCHVKEFQKAFSKEKTPFLSRSQQMHMNRH 960

Query: 961 FGV 964
           FGV
Sbjct: 961 FGV 963

BLAST of Carg08653 vs. NCBI nr
Match: XP_022936287.1 (mechanosensitive ion channel protein 8-like [Cucurbita moschata])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 631/686 (91.98%), Postives = 635/686 (92.57%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60
           MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENR PSSGSSGGE
Sbjct: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQPSSGSSGGE 60

Query: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120
           VLRCSSNYSFSGSSWSSPISK SRLMDPPEDDSSQKLETAVKDEGNNN+N+D TEDMPEE
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPEE 120

Query: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180
           YNQLKLSPLLGFQLVVFVLII SLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS
Sbjct: 121 YNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180

Query: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240
           SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK
Sbjct: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240

Query: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300
           ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL
Sbjct: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300

Query: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360
           FERPNSEDMMKDEQI DG AES KSGRVVCSGRLVNCNG RQLKVESKRKDEEIPVDELN
Sbjct: 301 FERPNSEDMMKDEQIGDGKAESSKSGRVVCSGRLVNCNGWRQLKVESKRKDEEIPVDELN 360

Query: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420
           KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV
Sbjct: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420

Query: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480
           AQRGSDCIY EDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS
Sbjct: 421 AQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480

Query: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540
           LNDANTAVDDLHNLLNVFVALTILVIWLIIFGT VLHFLIFITSQLLLLAFIFGNTCKTV
Sbjct: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFGNTCKTV 540

Query: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600
           FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN
Sbjct: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600

Query: 601 YYRSPDMGEG-----------------------------------------DVEDLEKVR 646
           YYRSPDMGEG                                         DVEDLEKVR
Sbjct: 601 YYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKVR 660

BLAST of Carg08653 vs. NCBI nr
Match: XP_023535846.1 (mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535847.1 mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 629/686 (91.69%), Postives = 634/686 (92.42%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60
           MAPKEDVILKVNSF NGDKELVEKKFQCLPERSLQTMRRRGKEFSEP+NR PSSGSSGGE
Sbjct: 1   MAPKEDVILKVNSFRNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPDNRQPSSGSSGGE 60

Query: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120
           VLRCSSNYSFSGSSWSSPISK SRLMDPPEDDSSQKLETAVKDEGNNNDN+D TEDMPEE
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNDNQDGTEDMPEE 120

Query: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180
           YNQLKLSPLLGFQLVVFVLII SLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS
Sbjct: 121 YNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180

Query: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240
           SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLW SLILLVWHFIFNDKVQRKTQSK
Sbjct: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWTSLILLVWHFIFNDKVQRKTQSK 240

Query: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300
           ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL
Sbjct: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300

Query: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360
           FERPNSEDMMKDEQI DG AES KSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN
Sbjct: 301 FERPNSEDMMKDEQIDDGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360

Query: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420
           KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV
Sbjct: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420

Query: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480
           AQRGSDCIY EDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS
Sbjct: 421 AQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480

Query: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540
           LNDANTAVDDLHNLLNVFVALTILVIWLIIFGT VLHFLIFITSQLLLLAFIFGNTCKTV
Sbjct: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFGNTCKTV 540

Query: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600
           FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN
Sbjct: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600

Query: 601 YYRSPDMGEG-----------------------------------------DVEDLEKVR 646
           YYRSPDMGEG                                         DVEDLEKVR
Sbjct: 601 YYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKVR 660

BLAST of Carg08653 vs. NCBI nr
Match: XP_022975440.1 (mechanosensitive ion channel protein 8-like [Cucurbita maxima])

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 625/686 (91.11%), Postives = 634/686 (92.42%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60
           MAPKEDVILKVNSFG+GDKELVEKKFQCLPERSLQTMRRRGKEFSEPENR  SSGSSGGE
Sbjct: 1   MAPKEDVILKVNSFGHGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQLSSGSSGGE 60

Query: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120
           VLRCSSN SF GSSWSSPISK SRLMDPPEDDSSQKLETAVKDEGNNN+N+D TEDMPEE
Sbjct: 61  VLRCSSNSSFPGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPEE 120

Query: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180
           YNQLKLSPLLGFQLVVFVLII SLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS
Sbjct: 121 YNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180

Query: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240
           SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK
Sbjct: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240

Query: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300
           ILPYVTKILICFLVAA+IWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL
Sbjct: 241 ILPYVTKILICFLVAATIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300

Query: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360
           FERPNSEDMMKDEQIADG AES KSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN
Sbjct: 301 FERPNSEDMMKDEQIADGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360

Query: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420
           KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSET+EQSLLQIR+ERQAKEAAGRIFRRV
Sbjct: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETKEQSLLQIRSERQAKEAAGRIFRRV 420

Query: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480
           AQRGSDCIY EDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS
Sbjct: 421 AQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480

Query: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540
           LNDANTAVDDLHNLLNVFVALTILVIWLI+FGT VLHFLIFITSQLLLLAFIFGNTCKTV
Sbjct: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIVFGTPVLHFLIFITSQLLLLAFIFGNTCKTV 540

Query: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600
           FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN
Sbjct: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600

Query: 601 YYRSPDMGEG-----------------------------------------DVEDLEKVR 646
           YYRSPDMGEG                                         DVEDLEKVR
Sbjct: 601 YYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKVR 660

BLAST of Carg08653 vs. NCBI nr
Match: KAG6591247.1 (Mechanosensitive ion channel protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 636/738 (86.18%), Postives = 654/738 (88.62%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60
           MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE
Sbjct: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60

Query: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120
           VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120

Query: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180
           YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS
Sbjct: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180

Query: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240
           SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQR TQSK
Sbjct: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRTTQSK 240

Query: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300
           ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLS    
Sbjct: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLS---- 300

Query: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360
                  DMMKDEQIADG AESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN
Sbjct: 301 -------DMMKDEQIADGKAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360

Query: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420
           KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV
Sbjct: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420

Query: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480
           AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS
Sbjct: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480

Query: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540
           LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV
Sbjct: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540

Query: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600
           FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN
Sbjct: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600

Query: 601 YYRSPDMGEG------DVEDLEKVRMSVGLTHKLNH--QNMKERWNRRAALIELNVEGRI 660
           YYRSPDMGEG           EKV +   L  ++    ++M E+W+    ++  +VE   
Sbjct: 601 YYRSPDMGEGVDFYIHISTPWEKVAL---LKERITRYVESMGEQWHPDPQIVMKDVE--- 660

Query: 661 FFFLQQPKLGAAVHGHGDYFRRRRSSSRPYPE------TEEPRGGTLQRQSRAVPPPPTL 720
                        H H     RR +++    +          +GGTLQRQ RAVPP PTL
Sbjct: 661 ---------DLEKHPHSRRRGRRSTTTAITSDGGAAVPNHTRKGGTLQRQPRAVPPLPTL 712

Query: 721 PVADPAARTVSQLSSAAL 725
           PVADPAART + + S A+
Sbjct: 721 PVADPAARTSALIGSPAV 712

BLAST of Carg08653 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 543.1 bits (1398), Expect = 6.3e-153
Identity = 303/648 (46.76%), Postives = 428/648 (66.05%), Query Frame = 0

Query: 60  EVLRCSSNYSFS-GSSWSSPISKNSRLMDPPEDDS-------SQKLETAVKDEGNNNDNK 119
           EV+RC+SN SF   S   S +   SRL DPP ++        S +L++ +  + +  D+ 
Sbjct: 222 EVVRCTSNMSFQRKSELISRVKTRSRLQDPPREEETPYSGWRSGQLKSGLLADIDEEDDP 281

Query: 120 DATEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVL 179
            A ED+P+EY + KL  +   Q +  V II +L C+  I   K+   W L LWKWE+ +L
Sbjct: 282 LAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLL 341

Query: 180 AVICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFND 239
            +ICG +VS   + ++V  IE NFLLRKRVLYFVYG+R+AV+NCLW+ L+LL WHF+F+ 
Sbjct: 342 VLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDK 401

Query: 240 KVQRKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYV 299
           KVQR+T+S+ LPYVTKIL+CFL++  +WL+KTLV+K+LAS FHV+ YF+R++EALF+QYV
Sbjct: 402 KVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQYV 461

Query: 300 IVTLSGLPLFERPNSEDMMKDEQIADGIAESYKSGR----VVC--------SGRLVNCNG 359
           I TLSG P+ E    E+  ++E+  D I +   +G      +C        SGR++N   
Sbjct: 462 IETLSGPPMIEMSRIEE--EEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRVMNPKL 521

Query: 360 SRQLKVESKRKDEEIPVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSET-EEQ 419
           S    +     D  I ++ L+++N KNISAWNM+R+M IVRN +L TLDE++L  T E++
Sbjct: 522 SP--IIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDE 581

Query: 420 SLLQIRNERQAKEAAGRIFRRVAQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCI 479
           S  QIR+E++AK AA +IF+ V QRG+  IY ED+MRF+ ++EA+ TM LF    E + I
Sbjct: 582 STRQIRSEKEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRI 641

Query: 480 DESSFREWMVNAFKQRKVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHF 539
            +S+ + W+VNAF++R+ L L+LND  TAV+ LH+++N+  A+ I+VIWL++        
Sbjct: 642 SKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKV 701

Query: 540 LIFITSQLLLLAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVF 599
           L+F++SQ++LLAFIFGNT KTVFE+IIFLF++HP+DVGDRCE+D V++VVEEM ILTTVF
Sbjct: 702 LLFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVF 761

Query: 600 LRYDNQRISYPNSVLATKAIGNYYRSPDMGEG---------------------------- 646
           LRYDN +I YPNS+L  K+I NYYRSPDMG+                             
Sbjct: 762 LRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNK 821

BLAST of Carg08653 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.1e-144
Identity = 302/694 (43.52%), Postives = 435/694 (62.68%), Query Frame = 0

Query: 48  ENRLPSSGSSGGEVLRCSSNYS--FSGSSWSSPISKNSRLMDPP--------EDDSSQKL 107
           ++R  ++ +  GEV++CS N +     SS    +   SRL DPP         D  S ++
Sbjct: 138 DSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQTADMKSGRI 197

Query: 108 E-----------TAVKDEG-NNNDNKDATEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLI 167
                        + K +G    D+  A ED+PEEY + KLS  +  + +  +LII   +
Sbjct: 198 PKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFV 257

Query: 168 CNRWISILKRETFWGLPLWKWELTVLAVICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFV 227
           C   I  L+++  W L LWKWE  VL +ICG +VSS  V ++V  IE NFLLRKRVLYFV
Sbjct: 258 CTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFV 317

Query: 228 YGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSKILPYVTKILICFLVAASIWLLKTLV 287
           YG+RKAV+NCLW+ L+LL WHF+F++KV +   +K L  VTKI +C LV   +WL+KTL+
Sbjct: 318 YGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLL 377

Query: 288 IKILASFFHVNNYFERLKEALFSQYVIVTLSGLPLFERPNSEDMMKDEQIADGIAESYKS 347
           +K+LAS FH++ YF+R++E+LF+QYVI TLSG PL E   +E+  ++E+I+  + +    
Sbjct: 378 VKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEE--EEERISVEVKKFQNP 437

Query: 348 GRVVCSGRLVNCNGSRQLKVESKR----------------KDEEIPVDELNKLNQKNISA 407
           G V         +G+++  +++ +                +++ I +D L+KLN KN+SA
Sbjct: 438 GGVEIQ------SGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSA 497

Query: 408 WNMRRMMNIVRNGALLTLDEKILSET-EEQSLLQIRNERQAKEAAGRIFRRVAQRGSDCI 467
           W M+R+MNI+RNG+L TLDE++   + ++    QIR+E +AK AA +IF  VA+ GS  I
Sbjct: 498 WKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFI 557

Query: 468 YKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLNDANTAV 527
           Y  D+MRF+  +EAL T+SLF   +ET  I +SS + W+VNAF++R+ L L+LND  TAV
Sbjct: 558 YANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAV 617

Query: 528 DDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFEAIIFLF 587
           + LH ++N+ V + ILVIWLII G     FL+ ++SQ++++AFIFGN CK VFE+II+LF
Sbjct: 618 NRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLF 677

Query: 588 VMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYYRSPDMG 647
           V+HPFDVGDRCE+DGV+MVVEEM ILTTVFLR+DNQ++ YPNS+L TK+IGNYYRSPDMG
Sbjct: 678 VIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMG 737

Query: 648 EG-----------------------------------------DVEDLEKVRMSVGLTHK 654
           +G                                         D+E L  VR++V  TH+
Sbjct: 738 DGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHR 797

BLAST of Carg08653 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.0e-143
Identity = 299/700 (42.71%), Postives = 424/700 (60.57%), Query Frame = 0

Query: 48  ENRLPSSGSSG--------GEVLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLET 107
           +NR    GSS          EVL+C S          S     SRL DPP        +T
Sbjct: 158 QNRTSLGGSSDEEGRNRDEAEVLKCGSK-----KPMLSRNKTKSRLQDPPTPTHPAIDKT 217

Query: 108 AVKD--------------------------EGNNNDNKDATEDMPEEYNQLKLSPLLGFQ 167
            +K                           E    ++    ED+PEE+ + KLS  +  +
Sbjct: 218 EMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLE 277

Query: 168 LVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVSSMAVGLMVKIIEM 227
            +  VLI+ SL+C+  I  L+R+T+W L LWKWE+TVL +ICG +VSS  V ++V ++E 
Sbjct: 278 WISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEK 337

Query: 228 NFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSKILPYVTKILICFL 287
           NF  RKRVLYFVYG+RK+V+NCLW+ L+LL WHF+F+ KV+R+T+S  L YVT++L+C L
Sbjct: 338 NFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLL 397

Query: 288 VAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPLFERPNSEDMMKDE 347
           VA  IWL+KT+++K+LAS FH++ YF+R++E+LF+QYVI TLSG PL E    E+  +++
Sbjct: 398 VALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEE--EEQ 457

Query: 348 QIADGIAESYK----------SGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELNKLN 407
           Q+A+ +    K             V    ++    G  ++  +     E I +D+L ++N
Sbjct: 458 QVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMN 517

Query: 408 QKNISAWNMRRMMNIVRNGALLTLDEKILSET-EEQSLLQIRNERQAKEAAGRIFRRVAQ 467
            KN+SAWNM+R+MNI+  GA+ TLD+ +   T E++    IR+E +AK AA +IF  V +
Sbjct: 518 TKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTE 577

Query: 468 RGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLN 527
            GS  IY ED +RF+ +EEA   M+LF   +E+  I +S  + W+V AF++R+ L L+LN
Sbjct: 578 PGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLN 637

Query: 528 DANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFE 587
           D  TAVD LH ++NV + + I++IWL+I G     FL+ ++SQLLL+AF+FGN+CKT+FE
Sbjct: 638 DTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFE 697

Query: 588 AIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYY 647
           AIIFLFVMHPFDVGDRCE+DGV++VVEEM ILTTVFLRYDNQ+I YPNSVL TK I NYY
Sbjct: 698 AIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYY 757

Query: 648 RSPDMGEG-----------------------------------------DVEDLEKVRMS 654
           RSPDMG+                                           ++DL  V+++
Sbjct: 758 RSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIA 817

BLAST of Carg08653 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 6.5e-142
Identity = 299/739 (40.46%), Postives = 438/739 (59.27%), Query Frame = 0

Query: 13  SFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGEVLRCSSNYSFSG 72
           S+ NG  + + ++   +   +L    + G    + ENR+      G EV++C+SN S   
Sbjct: 126 SYRNGTGDAIRRRQNRV---TLSPSVKDGDSSEDEENRV-----DGSEVVKCTSNRSTMR 185

Query: 73  SSWSSPISKNSRLMDPPEDD-------------------SSQKLETAVKDEGNNNDNKD- 132
           +     +   SRLMDPP                      S +  +    ++G + D ++ 
Sbjct: 186 TKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEE 245

Query: 133 ----ATEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWEL 192
               + ED+PE   + K+   +  + +  +LII SLIC+  I  L+ +T W L LWKWE+
Sbjct: 246 EDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEV 305

Query: 193 TVLAVICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFI 252
            VL +ICG +VSS  V L V  +E NFL RK+VLYFVYG+RK V+NCLW+ L+L+ WHF+
Sbjct: 306 MVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFL 365

Query: 253 FNDKVQRKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFS 312
           F+ KV+R+ +S +L YVTK+LIC LVA  IWL+KTL++K+LAS FH++ YF+R++E+LF+
Sbjct: 366 FDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFT 425

Query: 313 QYVIVTLSGLPLFERPNSEDMMKDE-----------------------QIADGIAESYKS 372
           QYVI TLSG P  E    E+ + ++                       Q+  G     KS
Sbjct: 426 QYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKS 485

Query: 373 GRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELNKLNQKNISAWNMRRMMNIVRNGALL 432
              V    +++ +GS++     +  +E I +D L ++N KN+SAW M+++MN+++ G L 
Sbjct: 486 PSRVGKSPVLSRSGSKK-----EGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLS 545

Query: 433 TLDEKILSET--EEQSLLQIRNERQAKEAAGRIFRRVAQRGSDCIYKEDVMRFMNKEEAL 492
           TLDE+I   T  E+    QIR+E +AK AA +IF+ VA+ GS  IY ED MRF++++E+ 
Sbjct: 546 TLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESE 605

Query: 493 VTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLNDANTAVDDLHNLLNVFVALTI 552
             M LF   +E   I +S  + W+VNAF++R+ L L+LND  TAV+ LH +++V V++ I
Sbjct: 606 RAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVI 665

Query: 553 LVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDG 612
           L+IWL+I G     FL+ I+SQLLL+ F+FGN+CKT+FEA+IF+FVMHPFDVGDRCE+DG
Sbjct: 666 LIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDG 725

Query: 613 VEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYYRSPDMGEG------------- 654
           V+M+VEEM ILTTVFLR+DNQ+I YPNS+L TK I NYYRSPDM +              
Sbjct: 726 VQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEK 785

BLAST of Carg08653 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 3.6e-140
Identity = 298/704 (42.33%), Postives = 428/704 (60.80%), Query Frame = 0

Query: 7   VILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGEVLRCSS 66
           V  K+NS G  +     +        S  TMR   ++  E E         G  V+RCS 
Sbjct: 109 VFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQLEDE---------GEVVVRCS- 168

Query: 67  NYSFSGSSWSSPISKNSRLMDPPEDD---------SSQKLETAV----KDEGNNNDNKDA 126
             S   +   S     SRL+DPP+++         +S +L + +     D+    D+  A
Sbjct: 169 --SVRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSA 228

Query: 127 TEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAV 186
            ED+P EY +LK+  +   Q +  + ++ +L+ +  +   +  T W L LWKWE+ +L +
Sbjct: 229 EEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVL 288

Query: 187 ICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKV 246
           ICG +VS   + ++V  IE NFLLRKRVLYFVYG++ AV+NCLW+ L+LL WHF+F+ KV
Sbjct: 289 ICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKV 348

Query: 247 QRKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIV 306
           +++TQS +L  ++KIL+CFL++  +WL+KTLV+K+LAS FHV+ YF+R++EALF  Y+I 
Sbjct: 349 EKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIE 408

Query: 307 TLSGLPLFERPNSEDMMKDEQIADGIAESYKSGR----VVCSGRLVNCNGSRQLK----- 366
           TLSG P+ E    E+  ++++  D I +  K G      +CS           +      
Sbjct: 409 TLSGPPMLELSRIEE--EEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTMNMKFSP 468

Query: 367 -VESKRKDEEIPVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSET-EEQSLLQ 426
            +     D  I +D+L+K+NQKN+SAWNM+R+M IVRN +L TLDE+ L  T E++S  Q
Sbjct: 469 IIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDESTRQ 528

Query: 427 IRNERQAKEAAGRIFRRVAQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESS 486
           IR+E++AK AA +IF+ VAQ G+  IY ED+MRF+  +EA+ TM LF     T+ I +S+
Sbjct: 529 IRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSA 588

Query: 487 FREWMVNAFKQRKVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFI 546
            + W+VNAF++R+ L L+LND  TAV+ LH++++   A+ I+VIWLI+       +L+F+
Sbjct: 589 LKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFL 648

Query: 547 TSQLLLLAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYD 606
           TSQ++LLAF+FGN+ KTVFE+IIFLF++HP+DVGDR  +D VEMVVEEM ILTTVFLR D
Sbjct: 649 TSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRAD 708

Query: 607 NQRISYPNSVLATKAIGNYYRSPDMGE--------------------------------- 646
           N +I YPN +L  KAI NY RSPDMG+                                 
Sbjct: 709 NLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYW 768

BLAST of Carg08653 vs. ExPASy TrEMBL
Match: A0A6J1F811 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111442956 PE=3 SV=1)

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 631/686 (91.98%), Postives = 635/686 (92.57%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60
           MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENR PSSGSSGGE
Sbjct: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQPSSGSSGGE 60

Query: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120
           VLRCSSNYSFSGSSWSSPISK SRLMDPPEDDSSQKLETAVKDEGNNN+N+D TEDMPEE
Sbjct: 61  VLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPEE 120

Query: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180
           YNQLKLSPLLGFQLVVFVLII SLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS
Sbjct: 121 YNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180

Query: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240
           SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK
Sbjct: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240

Query: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300
           ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL
Sbjct: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300

Query: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360
           FERPNSEDMMKDEQI DG AES KSGRVVCSGRLVNCNG RQLKVESKRKDEEIPVDELN
Sbjct: 301 FERPNSEDMMKDEQIGDGKAESSKSGRVVCSGRLVNCNGWRQLKVESKRKDEEIPVDELN 360

Query: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420
           KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV
Sbjct: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420

Query: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480
           AQRGSDCIY EDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS
Sbjct: 421 AQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480

Query: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540
           LNDANTAVDDLHNLLNVFVALTILVIWLIIFGT VLHFLIFITSQLLLLAFIFGNTCKTV
Sbjct: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFGNTCKTV 540

Query: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600
           FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN
Sbjct: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600

Query: 601 YYRSPDMGEG-----------------------------------------DVEDLEKVR 646
           YYRSPDMGEG                                         DVEDLEKVR
Sbjct: 601 YYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKVR 660

BLAST of Carg08653 vs. ExPASy TrEMBL
Match: A0A6J1IGQ2 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111474765 PE=3 SV=1)

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 625/686 (91.11%), Postives = 634/686 (92.42%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGE 60
           MAPKEDVILKVNSFG+GDKELVEKKFQCLPERSLQTMRRRGKEFSEPENR  SSGSSGGE
Sbjct: 1   MAPKEDVILKVNSFGHGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQLSSGSSGGE 60

Query: 61  VLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPEE 120
           VLRCSSN SF GSSWSSPISK SRLMDPPEDDSSQKLETAVKDEGNNN+N+D TEDMPEE
Sbjct: 61  VLRCSSNSSFPGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPEE 120

Query: 121 YNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180
           YNQLKLSPLLGFQLVVFVLII SLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS
Sbjct: 121 YNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVS 180

Query: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240
           SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK
Sbjct: 181 SMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSK 240

Query: 241 ILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300
           ILPYVTKILICFLVAA+IWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL
Sbjct: 241 ILPYVTKILICFLVAATIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPL 300

Query: 301 FERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360
           FERPNSEDMMKDEQIADG AES KSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN
Sbjct: 301 FERPNSEDMMKDEQIADGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELN 360

Query: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRRV 420
           KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSET+EQSLLQIR+ERQAKEAAGRIFRRV
Sbjct: 361 KLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETKEQSLLQIRSERQAKEAAGRIFRRV 420

Query: 421 AQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480
           AQRGSDCIY EDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS
Sbjct: 421 AQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLS 480

Query: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTV 540
           LNDANTAVDDLHNLLNVFVALTILVIWLI+FGT VLHFLIFITSQLLLLAFIFGNTCKTV
Sbjct: 481 LNDANTAVDDLHNLLNVFVALTILVIWLIVFGTPVLHFLIFITSQLLLLAFIFGNTCKTV 540

Query: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600
           FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN
Sbjct: 541 FEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGN 600

Query: 601 YYRSPDMGEG-----------------------------------------DVEDLEKVR 646
           YYRSPDMGEG                                         DVEDLEKVR
Sbjct: 601 YYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKVR 660

BLAST of Carg08653 vs. ExPASy TrEMBL
Match: A0A6J1CGD4 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011429 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 8.8e-291
Identity = 530/687 (77.15%), Postives = 585/687 (85.15%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNG-DKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGG 60
           MAP+EDV+LK++SFGNG DKE  EK FQCLPERS QTMRRRGKE S+ ENR PSS S GG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPE 120
           EVLRCSSN SF G SWSSPISK SRLMDPPE+DSSQKLETAV +EG    N+D TEDMPE
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGK---NQDETEDMPE 120

Query: 121 EYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMV 180
           EYNQLKLSP    Q V+FV++I  LICNRW+ ILKR++ W LPLWKWELTVLA+ICG ++
Sbjct: 121 EYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLI 180

Query: 181 SSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQS 240
           S+ AVGL+VKIIE NFLLRKRVLYFVYGLR+AV+NCLW+SL+LLVWHFIFN+KVQR T+S
Sbjct: 181 STWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRS 240

Query: 241 KILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLP 300
           KILPY+TKILICFLV ASIWLLKTL +KILASFFHVNNYFERLKEALFSQYVIVTLSG P
Sbjct: 241 KILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSP 300

Query: 301 LFERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDEL 360
           LFER N+EDM+KD Q ADG  ES KSGR++CSGRLVNC+GSRQLK ES R+DEEIPVD+L
Sbjct: 301 LFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQL 360

Query: 361 NKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRR 420
           NKLNQ NISAWNMRRMMNIVRNGALLTLDEKILSET+E+ LLQIR+ERQAKEAAG IFRR
Sbjct: 361 NKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRR 420

Query: 421 VAQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVL 480
           V++RGS+ I  +DVMRFMNKEEAL+TMSLFG  AETQCIDE SFREWMVNAF QRKVLVL
Sbjct: 421 VSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVL 480

Query: 481 SLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKT 540
           SLNDANTAVD LHNLLN+FVA+TILVIWLII GT +LHFLIFI+SQLLLL FIFGNTCKT
Sbjct: 481 SLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKT 540

Query: 541 VFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIG 600
           VFEAIIFLFVMHPFDVGDRCEVDGV+MVVEEM+ILTTVFLRYDNQ+I+YPNSVLATKAIG
Sbjct: 541 VFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIG 600

Query: 601 NYYRSPDMGEG-----------------------------------------DVEDLEKV 646
           NYYRSPDMGEG                                         DVEDLEKV
Sbjct: 601 NYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKV 660

BLAST of Carg08653 vs. ExPASy TrEMBL
Match: A0A6J1CH06 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011429 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 3.8e-270
Identity = 501/687 (72.93%), Postives = 554/687 (80.64%), Query Frame = 0

Query: 1   MAPKEDVILKVNSFGNG-DKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGG 60
           MAP+EDV+LK++SFGNG DKE  EK FQCLPERS QTMRRRGKE S+ ENR PSS S GG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLETAVKDEGNNNDNKDATEDMPE 120
           EVLRCSSN SF G SWSSPISK SRLMDPPE+DSSQKLETAV +EG    N+D TEDMPE
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGK---NQDETEDMPE 120

Query: 121 EYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMV 180
           EYNQLKLSP    Q V+FV++I  LICNRW+ ILKR++ W LPLWKWELTVLA+ICG ++
Sbjct: 121 EYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLI 180

Query: 181 SSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQS 240
           S+ AVGL+VKIIE NFLLRKRVLYFVYGLR+AV+NCLW+SL+LLVWHFIFN+KVQR T+S
Sbjct: 181 STWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRS 240

Query: 241 KILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLP 300
           KILPY+TKILICFLV ASIWLLKTL +KILASFFHVNNYFERLKEALFSQYVIVTLSG P
Sbjct: 241 KILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSP 300

Query: 301 LFERPNSEDMMKDEQIADGIAESYKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDEL 360
           LFER N+EDM+KD Q ADG  ES KSGR++CSGRLVNC+GSRQLK ES R+DEEIPVD+L
Sbjct: 301 LFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQL 360

Query: 361 NKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRR 420
           NKLNQ NISAWNMRRMMNIVRNGALLTLDEKILSET+E+ LLQIR+ERQAKEAAG IFRR
Sbjct: 361 NKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRR 420

Query: 421 VAQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVL 480
           V++RGS+ I  +DVMRFMNKEEAL+TMSLFG  AETQCIDE SFREWMVNAF QRKVLVL
Sbjct: 421 VSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVL 480

Query: 481 SLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKT 540
           SLNDANTAVD LHNLLN+FVA+TIL                                  T
Sbjct: 481 SLNDANTAVDGLHNLLNIFVAVTIL----------------------------------T 540

Query: 541 VFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIG 600
           VFEAIIFLFVMHPFDVGDRCEVDGV+MVVEEM+ILTTVFLRYDNQ+I+YPNSVLATKAIG
Sbjct: 541 VFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIG 600

Query: 601 NYYRSPDMGEG-----------------------------------------DVEDLEKV 646
           NYYRSPDMGEG                                         DVEDLEKV
Sbjct: 601 NYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKV 650

BLAST of Carg08653 vs. ExPASy TrEMBL
Match: A0A6J5TPN6 (Mechanosensitive ion channel protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7634 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 9.6e-189
Identity = 381/726 (52.48%), Postives = 497/726 (68.46%), Query Frame = 0

Query: 1   MAPKE-DVILKVNSFG----------NGDKELVEKKFQCLPERSLQTMRRRGKEFSEPEN 60
           MA +E  V++K+++ G          NG+           PE+  ++ RRR KE   P  
Sbjct: 1   MAQREGQVVVKIDNCGASCSKETTKINGEASPTTTTVGGSPEKPTESFRRRSKEL--PLT 60

Query: 61  RLPSSGSSGGEVLRCSSNYSFSGSSWSSPISK-NSRLMDPPEDD--SSQKLETAVKDEGN 120
             PS+ S G EVLRCSSN SFS +SW  P+SK  SRL+DPPE+    S ++  + +  G 
Sbjct: 61  DAPSN-SGGAEVLRCSSNASFSRNSWKPPMSKTKSRLLDPPEESCLKSDRVAGSGRALGK 120

Query: 121 NNDN--KDA-TEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPL 180
           N+DN   DA  ED+PEEY ++K S L   Q V  V +I +L+CN WI I+KR+T W LPL
Sbjct: 121 NDDNALDDADIEDIPEEYKRIKFSALTLLQWVSLVFVIAALVCNLWIPIIKRQTLWDLPL 180

Query: 181 WKWELTVLAVICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILL 240
           WKWEL VLA+ICG +VS   + ++V  +E NFLLRKRVLYFVYGLRK+V+NCLW+ L+L+
Sbjct: 181 WKWELLVLALICGRLVSGWGIRVVVFFVERNFLLRKRVLYFVYGLRKSVQNCLWLGLVLV 240

Query: 241 VWHFIFNDKVQRKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLK 300
           VWHFIF+ KV+ KTQS+ILPYVTK+LICFLV   IWLLKT+++K+LA  FHVN +FER++
Sbjct: 241 VWHFIFDKKVEEKTQSRILPYVTKVLICFLVGTLIWLLKTILVKVLALSFHVNAFFERIQ 300

Query: 301 EALFSQYVIVTLSGLPLFERPNSEDMMK---------------DEQIADGIAESYKSGRV 360
           EALF+QYVI TLSG PLFER ++E+  K                 ++   + +  +SGRV
Sbjct: 301 EALFNQYVIETLSGPPLFERKHTEEEEKVAAEIREFQNAGATMPRELRASLLQRARSGRV 360

Query: 361 VCSGRLVNCNGSRQLKVE---SKRKDEEIPVDELNKLNQKNISAWNMRRMMNIVRNGALL 420
           + SGR  +    +  +V    S  +DEEIPVD L KLNQKN+SAWNMRRM+NI+R+G+L 
Sbjct: 361 IGSGRQNSPRVGKSPRVSRPTSGSQDEEIPVDHLQKLNQKNVSAWNMRRMVNIIRHGSLT 420

Query: 421 TLDEKIL-SETEEQSLLQIRNERQAKEAAGRIFRRVAQRGSDCIYKEDVMRFMNKEEALV 480
           TLDE+IL S+ E+ S L+I+ E QAKEAA +IF +VA+ GS  IY ED+M FM+K+EAL 
Sbjct: 421 TLDEQILNSDIEDDSSLEIKTECQAKEAAKKIFLKVAKPGSSYIYLEDLMPFMHKDEALK 480

Query: 481 TMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLNDANTAVDDLHNLLNVFVALTIL 540
           T+ LFG   E+  I +S+ R+W+V+AF++R+ L LSLND  TAVD+LHN+LN+ VA+ I+
Sbjct: 481 TIHLFGAANESDRISKSALRDWVVSAFRERRALALSLNDTKTAVDELHNILNIIVAVIIV 540

Query: 541 VIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGV 600
           +IWLII G  V HFL+ I+SQLLL+ FIFGNTCKTVFEAIIFLFVMHPFDVGDRCEV+GV
Sbjct: 541 IIWLIILGIPVTHFLLLISSQLLLVVFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVEGV 600

Query: 601 EMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYYRSPDMGEG-------------- 650
           +MVVEEM ILTTVFL++D+Q+I YPNS+LATK I NY+RSPDMG+               
Sbjct: 601 QMVVEEMNILTTVFLKFDHQKIIYPNSILATKPIANYHRSPDMGDAVDFCVHISTPLEKL 660

BLAST of Carg08653 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 513.8 bits (1322), Expect = 2.9e-145
Identity = 302/694 (43.52%), Postives = 435/694 (62.68%), Query Frame = 0

Query: 48  ENRLPSSGSSGGEVLRCSSNYS--FSGSSWSSPISKNSRLMDPP--------EDDSSQKL 107
           ++R  ++ +  GEV++CS N +     SS    +   SRL DPP         D  S ++
Sbjct: 138 DSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQTADMKSGRI 197

Query: 108 E-----------TAVKDEG-NNNDNKDATEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLI 167
                        + K +G    D+  A ED+PEEY + KLS  +  + +  +LII   +
Sbjct: 198 PKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFV 257

Query: 168 CNRWISILKRETFWGLPLWKWELTVLAVICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFV 227
           C   I  L+++  W L LWKWE  VL +ICG +VSS  V ++V  IE NFLLRKRVLYFV
Sbjct: 258 CTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFV 317

Query: 228 YGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSKILPYVTKILICFLVAASIWLLKTLV 287
           YG+RKAV+NCLW+ L+LL WHF+F++KV +   +K L  VTKI +C LV   +WL+KTL+
Sbjct: 318 YGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLL 377

Query: 288 IKILASFFHVNNYFERLKEALFSQYVIVTLSGLPLFERPNSEDMMKDEQIADGIAESYKS 347
           +K+LAS FH++ YF+R++E+LF+QYVI TLSG PL E   +E+  ++E+I+  + +    
Sbjct: 378 VKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEE--EEERISVEVKKFQNP 437

Query: 348 GRVVCSGRLVNCNGSRQLKVESKR----------------KDEEIPVDELNKLNQKNISA 407
           G V         +G+++  +++ +                +++ I +D L+KLN KN+SA
Sbjct: 438 GGVEIQ------SGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSA 497

Query: 408 WNMRRMMNIVRNGALLTLDEKILSET-EEQSLLQIRNERQAKEAAGRIFRRVAQRGSDCI 467
           W M+R+MNI+RNG+L TLDE++   + ++    QIR+E +AK AA +IF  VA+ GS  I
Sbjct: 498 WKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFI 557

Query: 468 YKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLNDANTAV 527
           Y  D+MRF+  +EAL T+SLF   +ET  I +SS + W+VNAF++R+ L L+LND  TAV
Sbjct: 558 YANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAV 617

Query: 528 DDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFEAIIFLF 587
           + LH ++N+ V + ILVIWLII G     FL+ ++SQ++++AFIFGN CK VFE+II+LF
Sbjct: 618 NRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLF 677

Query: 588 VMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYYRSPDMG 647
           V+HPFDVGDRCE+DGV+MVVEEM ILTTVFLR+DNQ++ YPNS+L TK+IGNYYRSPDMG
Sbjct: 678 VIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMG 737

Query: 648 EG-----------------------------------------DVEDLEKVRMSVGLTHK 654
           +G                                         D+E L  VR++V  TH+
Sbjct: 738 DGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHR 797

BLAST of Carg08653 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 511.5 bits (1316), Expect = 1.4e-144
Identity = 299/700 (42.71%), Postives = 424/700 (60.57%), Query Frame = 0

Query: 48  ENRLPSSGSSG--------GEVLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLET 107
           +NR    GSS          EVL+C S          S     SRL DPP        +T
Sbjct: 158 QNRTSLGGSSDEEGRNRDEAEVLKCGSK-----KPMLSRNKTKSRLQDPPTPTHPAIDKT 217

Query: 108 AVKD--------------------------EGNNNDNKDATEDMPEEYNQLKLSPLLGFQ 167
            +K                           E    ++    ED+PEE+ + KLS  +  +
Sbjct: 218 EMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLE 277

Query: 168 LVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVSSMAVGLMVKIIEM 227
            +  VLI+ SL+C+  I  L+R+T+W L LWKWE+TVL +ICG +VSS  V ++V ++E 
Sbjct: 278 WISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEK 337

Query: 228 NFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSKILPYVTKILICFL 287
           NF  RKRVLYFVYG+RK+V+NCLW+ L+LL WHF+F+ KV+R+T+S  L YVT++L+C L
Sbjct: 338 NFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLL 397

Query: 288 VAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPLFERPNSEDMMKDE 347
           VA  IWL+KT+++K+LAS FH++ YF+R++E+LF+QYVI TLSG PL E    E+  +++
Sbjct: 398 VALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEE--EEQ 457

Query: 348 QIADGIAESYK----------SGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELNKLN 407
           Q+A+ +    K             V    ++    G  ++  +     E I +D+L ++N
Sbjct: 458 QVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMN 517

Query: 408 QKNISAWNMRRMMNIVRNGALLTLDEKILSET-EEQSLLQIRNERQAKEAAGRIFRRVAQ 467
            KN+SAWNM+R+MNI+  GA+ TLD+ +   T E++    IR+E +AK AA +IF  V +
Sbjct: 518 TKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTE 577

Query: 468 RGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLN 527
            GS  IY ED +RF+ +EEA   M+LF   +E+  I +S  + W+V AF++R+ L L+LN
Sbjct: 578 PGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLN 637

Query: 528 DANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFE 587
           D  TAVD LH ++NV + + I++IWL+I G     FL+ ++SQLLL+AF+FGN+CKT+FE
Sbjct: 638 DTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFE 697

Query: 588 AIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYY 647
           AIIFLFVMHPFDVGDRCE+DGV++VVEEM ILTTVFLRYDNQ+I YPNSVL TK I NYY
Sbjct: 698 AIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYY 757

Query: 648 RSPDMGEG-----------------------------------------DVEDLEKVRMS 654
           RSPDMG+                                           ++DL  V+++
Sbjct: 758 RSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIA 817

BLAST of Carg08653 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 506.5 bits (1303), Expect = 4.6e-143
Identity = 299/739 (40.46%), Postives = 438/739 (59.27%), Query Frame = 0

Query: 13  SFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGEVLRCSSNYSFSG 72
           S+ NG  + + ++   +   +L    + G    + ENR+      G EV++C+SN S   
Sbjct: 126 SYRNGTGDAIRRRQNRV---TLSPSVKDGDSSEDEENRV-----DGSEVVKCTSNRSTMR 185

Query: 73  SSWSSPISKNSRLMDPPEDD-------------------SSQKLETAVKDEGNNNDNKD- 132
           +     +   SRLMDPP                      S +  +    ++G + D ++ 
Sbjct: 186 TKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEE 245

Query: 133 ----ATEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWEL 192
               + ED+PE   + K+   +  + +  +LII SLIC+  I  L+ +T W L LWKWE+
Sbjct: 246 EDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEV 305

Query: 193 TVLAVICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFI 252
            VL +ICG +VSS  V L V  +E NFL RK+VLYFVYG+RK V+NCLW+ L+L+ WHF+
Sbjct: 306 MVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFL 365

Query: 253 FNDKVQRKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFS 312
           F+ KV+R+ +S +L YVTK+LIC LVA  IWL+KTL++K+LAS FH++ YF+R++E+LF+
Sbjct: 366 FDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFT 425

Query: 313 QYVIVTLSGLPLFERPNSEDMMKDE-----------------------QIADGIAESYKS 372
           QYVI TLSG P  E    E+ + ++                       Q+  G     KS
Sbjct: 426 QYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKS 485

Query: 373 GRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELNKLNQKNISAWNMRRMMNIVRNGALL 432
              V    +++ +GS++     +  +E I +D L ++N KN+SAW M+++MN+++ G L 
Sbjct: 486 PSRVGKSPVLSRSGSKK-----EGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLS 545

Query: 433 TLDEKILSET--EEQSLLQIRNERQAKEAAGRIFRRVAQRGSDCIYKEDVMRFMNKEEAL 492
           TLDE+I   T  E+    QIR+E +AK AA +IF+ VA+ GS  IY ED MRF++++E+ 
Sbjct: 546 TLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESE 605

Query: 493 VTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLNDANTAVDDLHNLLNVFVALTI 552
             M LF   +E   I +S  + W+VNAF++R+ L L+LND  TAV+ LH +++V V++ I
Sbjct: 606 RAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVI 665

Query: 553 LVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDG 612
           L+IWL+I G     FL+ I+SQLLL+ F+FGN+CKT+FEA+IF+FVMHPFDVGDRCE+DG
Sbjct: 666 LIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDG 725

Query: 613 VEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYYRSPDMGEG------------- 654
           V+M+VEEM ILTTVFLR+DNQ+I YPNS+L TK I NYYRSPDM +              
Sbjct: 726 VQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEK 785

BLAST of Carg08653 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 2.5e-141
Identity = 298/704 (42.33%), Postives = 428/704 (60.80%), Query Frame = 0

Query: 7   VILKVNSFGNGDKELVEKKFQCLPERSLQTMRRRGKEFSEPENRLPSSGSSGGEVLRCSS 66
           V  K+NS G  +     +        S  TMR   ++  E E         G  V+RCS 
Sbjct: 109 VFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQLEDE---------GEVVVRCS- 168

Query: 67  NYSFSGSSWSSPISKNSRLMDPPEDD---------SSQKLETAV----KDEGNNNDNKDA 126
             S   +   S     SRL+DPP+++         +S +L + +     D+    D+  A
Sbjct: 169 --SVRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSA 228

Query: 127 TEDMPEEYNQLKLSPLLGFQLVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAV 186
            ED+P EY +LK+  +   Q +  + ++ +L+ +  +   +  T W L LWKWE+ +L +
Sbjct: 229 EEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVL 288

Query: 187 ICGHMVSSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKV 246
           ICG +VS   + ++V  IE NFLLRKRVLYFVYG++ AV+NCLW+ L+LL WHF+F+ KV
Sbjct: 289 ICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKV 348

Query: 247 QRKTQSKILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIV 306
           +++TQS +L  ++KIL+CFL++  +WL+KTLV+K+LAS FHV+ YF+R++EALF  Y+I 
Sbjct: 349 EKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIE 408

Query: 307 TLSGLPLFERPNSEDMMKDEQIADGIAESYKSGR----VVCSGRLVNCNGSRQLK----- 366
           TLSG P+ E    E+  ++++  D I +  K G      +CS           +      
Sbjct: 409 TLSGPPMLELSRIEE--EEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTMNMKFSP 468

Query: 367 -VESKRKDEEIPVDELNKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSET-EEQSLLQ 426
            +     D  I +D+L+K+NQKN+SAWNM+R+M IVRN +L TLDE+ L  T E++S  Q
Sbjct: 469 IIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDESTRQ 528

Query: 427 IRNERQAKEAAGRIFRRVAQRGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESS 486
           IR+E++AK AA +IF+ VAQ G+  IY ED+MRF+  +EA+ TM LF     T+ I +S+
Sbjct: 529 IRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSA 588

Query: 487 FREWMVNAFKQRKVLVLSLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFI 546
            + W+VNAF++R+ L L+LND  TAV+ LH++++   A+ I+VIWLI+       +L+F+
Sbjct: 589 LKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFL 648

Query: 547 TSQLLLLAFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYD 606
           TSQ++LLAF+FGN+ KTVFE+IIFLF++HP+DVGDR  +D VEMVVEEM ILTTVFLR D
Sbjct: 649 TSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRAD 708

Query: 607 NQRISYPNSVLATKAIGNYYRSPDMGE--------------------------------- 646
           N +I YPN +L  KAI NY RSPDMG+                                 
Sbjct: 709 NLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYW 768

BLAST of Carg08653 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 449.5 bits (1155), Expect = 6.7e-126
Identity = 279/700 (39.86%), Postives = 394/700 (56.29%), Query Frame = 0

Query: 48  ENRLPSSGSSG--------GEVLRCSSNYSFSGSSWSSPISKNSRLMDPPEDDSSQKLET 107
           +NR    GSS          EVL+C S          S     SRL DPP        +T
Sbjct: 158 QNRTSLGGSSDEEGRNRDEAEVLKCGSK-----KPMLSRNKTKSRLQDPPTPTHPAIDKT 217

Query: 108 AVKD--------------------------EGNNNDNKDATEDMPEEYNQLKLSPLLGFQ 167
            +K                           E    ++    ED+PEE+ + KLS  +  +
Sbjct: 218 EMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLE 277

Query: 168 LVVFVLIIGSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMVSSMAVGLMVKIIEM 227
            +  VLI+ SL+C+  I  L+R+T+W L LWKWE+TVL +ICG +VSS  V ++V ++E 
Sbjct: 278 WISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEK 337

Query: 228 NFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQSKILPYVTKILICFL 287
           NF  RKRVLYFVYG+RK+V+NCLW+ L+LL WHF+F+ KV+R+T+S  L           
Sbjct: 338 NFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTAL----------- 397

Query: 288 VAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLPLFERPNSEDMMKDE 347
                                   YF+R++E+LF+QYVI TLSG PL E    E+  +++
Sbjct: 398 ----------------------RTYFDRIQESLFTQYVIETLSGPPLMEIQRMEE--EEQ 457

Query: 348 QIADGIAESYK----------SGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDELNKLN 407
           Q+A+ +    K             V    ++    G  ++  +     E I +D+L ++N
Sbjct: 458 QVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMN 517

Query: 408 QKNISAWNMRRMMNIVRNGALLTLDEKILSET-EEQSLLQIRNERQAKEAAGRIFRRVAQ 467
            KN+SAWNM+R+MNI+  GA+ TLD+ +   T E++    IR+E +AK AA +IF  V +
Sbjct: 518 TKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAARKIFHNVTE 577

Query: 468 RGSDCIYKEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVLSLN 527
            GS  IY ED +RF+ +EEA   M+LF   +E+  I +S  + W+  AF++R+ L L+LN
Sbjct: 578 PGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWV--AFRERRALALTLN 637

Query: 528 DANTAVDDLHNLLNVFVALTILVIWLIIFGTRVLHFLIFITSQLLLLAFIFGNTCKTVFE 587
           D  TAVD LH ++NV + + I++IWL+I G     FL+ ++SQLLL+AF+FGN+CKT+FE
Sbjct: 638 DTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFE 697

Query: 588 AIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIGNYY 647
           AIIFLFVMHPFDVGDRCE+DGV++VVEEM ILTTVFLRYDNQ+I YPNSVL TK I NYY
Sbjct: 698 AIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYY 757

Query: 648 RSPDMGEG-----------------------------------------DVEDLEKVRMS 654
           RSPDMG+                                           ++DL  V+++
Sbjct: 758 RSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIA 815

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024130.10.0e+00100.00Mechanosensitive ion channel protein 8, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022936287.10.0e+0091.98mechanosensitive ion channel protein 8-like [Cucurbita moschata][more]
XP_023535846.10.0e+0091.69mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_022975440.10.0e+0091.11mechanosensitive ion channel protein 8-like [Cucurbita maxima][more]
KAG6591247.10.0e+0086.18Mechanosensitive ion channel protein 8, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
F4IME26.3e-15346.76Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 P... [more]
Q9SYM14.1e-14443.52Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LH742.0e-14342.71Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
Q9LPG36.5e-14240.46Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
F4IME13.6e-14042.33Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 P... [more]
Match NameE-valueIdentityDescription
A0A6J1F8110.0e+0091.98Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC1114429... [more]
A0A6J1IGQ20.0e+0091.11Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111474765... [more]
A0A6J1CGD48.8e-29177.15Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1CH063.8e-27072.93Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J5TPN69.6e-18952.48Mechanosensitive ion channel protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCU... [more]
Match NameE-valueIdentityDescription
AT1G78610.12.9e-14543.52mechanosensitive channel of small conductance-like 6 [more]
AT3G14810.11.4e-14442.71mechanosensitive channel of small conductance-like 5 [more]
AT1G53470.14.6e-14340.46mechanosensitive channel of small conductance-like 4 [more]
AT2G17000.12.5e-14142.33Mechanosensitive ion channel family protein [more]
AT3G14810.26.7e-12639.86mechanosensitive channel of small conductance-like 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 493..604
e-value: 1.4E-12
score: 47.6
NoneNo IPR availableGENE3D3.10.20.90coord: 843..944
e-value: 2.4E-29
score: 103.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 672..710
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..115
NoneNo IPR availablePANTHERPTHR31618:SF8MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 36..609
coord: 611..648
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 552..601
e-value: 5.5E-12
score: 47.4
IPR040610SNRNP25, ubiquitin-like domainPFAMPF18036Ubiquitin_4coord: 849..935
e-value: 7.1E-24
score: 83.7
IPR040610SNRNP25, ubiquitin-like domainCDDcd17058Ubl_SNRNP25coord: 848..935
e-value: 4.8238E-29
score: 109.145
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 36..609
coord: 611..648
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 542..601
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 844..933

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08653-RACarg08653-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008381 mechanosensitive ion channel activity