Carg08564 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08564
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionlong chain acyl-CoA synthetase 4-like
LocationCarg_Chr02: 4942941 .. 4948100 (-)
RNA-Seq ExpressionCarg08564
SyntenyCarg08564
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACATTGCGCCCACGTCGTCTCCAGATTTCAACCCTAAATAAAGTTCTCAGGTTTTGGGAATTTCTTGAAATTTCTTGAAGTTTCAGAAGAAGAACAAGAAGAACAATGCAGATGAAGTATGTGATTCAGATGGAGGAGGCCAAGGAAGCCGCCGAGGACCGCCCATCGGTGGGGGCGGTTTACCGGAGCATCTTTGCCAAAGATGGGTTTCCGCCTCCGGTTGAAGGCTTGGATACCTGCTGGGATATATTTCGGTATGATAACAATTTATCTTTTATTTGTTAATCTGTTGTTTTATGGATGAGAAACCCCATGAAGCAAAATCTGTGAAATCTGTTTTGTTGAATCTTTTTCGATCCCTAATAGGAGAAATTATAGATTCATTTAAGGGTTCTTGAGCAAAATGTGAACACTTCATGGCTTTAATAAGCTTGGATTTAGAAACTTTTTAAATGTTCTCTAAACACTTGTGTTCATAATGCTTGATTCAGAATGTCTGTGGAGAAAAATCCTGGAAATCGGATGCTTGGACGTCGAAAAATAGTTGAAGGCAATGTAGTAAGTTACTGATAAATGAGTAGCCATGGCATTGAACTCAAGAGGGTAAAGAGTAATGGTGGTTGCTGTTGATCTGTAGGCTAATGCTGGTGAATATCAGTGGCTAACTTACAAAGAAGTCTATGACTTGGTGTTGAAAATTGGGAATGCAATTCGCAGCATGGGTTACGGACCAGTAAGTTTTTGAAGACGTTTATGCTCGTACAATGCTCTGCGTGGTTCGGAGTTAACGTTTATTATATGTTGTGGTGAGTTTACAGGGAGAAAAATGTGGGATTTATGGTGCTAATTGCTCTGAGTGGATCATTAGCATGGAGGTAATTAGTTCATTGTTTCCTGTTTGCTAGCAGTATGCAGATATCTAAGAGGCTATTTGGTGTTGTTTTTTTTTTTTTTTTACAGGCTTGCAATGCTCATGGGCTTTATTGTGTTCCTTTATATGATACTTTAGGTAAAAGACATGCTTCAAATGTGATTTTTGGTATTAAACTTTATATACGTTTCATTTTCGTTCATGAACTTTGCTGTCGTTACAAATGGGTTGGGTTGGGTTGTTACAAATGGTATTAGAGCTAGAACATCGGGCGGTGTGCCAACGAGGACGTTGGGCCCCCAAGGAGGGTGGATTGTGAGATCTCACATTGGTTGGAGAGAGAGGGAACAAAACATTTCTTGTAAGGGGGTGGAAACCTCTCCCTAGTAGACATGTTTTAAAACCGTGAGGCTAAGGTGATACGTAACGGGCTAAAGCGAACAATATCTCCTAACGATGAGCTCGGGTTGTTACAGTTCCTTATGAAAATTTAGAAGGTTTAAAAGTTTAGAGATCAAAATGGAATGCGAACATTTATAAGGTTTGAAATAGTATTTGGAGCTCAAAATAACGAATGATTCTTCTCTGAGCGTTTTCTCTATTATCCTGCTCCGTTTCCTATACAATATGAAAGCCGTTTCGGATTCAAACTTGTTGTAATATGTTTCAGGTGCTGGTGCCATAGAATTTATAATCTGTCATGCAGAGATTTCCATTGCTTTTGTAGAAGAGAAGAAGATCTGTGAGGTTCATAGCCATCATTTTTGCAACTAATTACGAGCCATATAGCTTCAACTAAAATGTTTCTCATATTTACTTTCAACTCTCTCTCTTATTCAGCTGTTGAAAACACTCCCCAACACAGCAAAGACTCTTAAAAGTAAGCTTTTATTCATTTTCTTCATTTTCTTCAATTTATAACATTTATTTTTGCTATGCAGCAATTGTGAGCTTTGGTAACGTTTCGAATGATCATAAGGAAGAAGTCGACAAGTTCGGGTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGTGAGTTTTATAAGTATATGTACGTCTGTGTTCGATTTGTAAAAATAACTACGTAGTCTTGGAAAGATTTAGACATCTACCGCTGAACTTCGACTCGGTTTCACAACTCCAAGCTATACAAATCTAGCCTGTGAACAAATATGGGGTGACAATTTATACCATTTTTGTAATAGCCCAAGCTCACGGCTAGCAGAAATTGTCTTCTTTAGGCTTTCCATTCCGGGCTTCCCCTCAAGGTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACAACCTTATAAAGGATGTTTTGTTCCTCTTTTCAACCGATATGGGATCTCACAATTCACCCCCTTCGGGGCCCAGCATCCTTGCTGACATATTGCCCCGGTGTCTGACTCTGATACCATTTGTAACAGCCCAACCCCACCACTATCAAATATTGTTCTCTTCGGGCTTTTCCCTTCTGGGCTTCCCTTCAAGGTTTTAAAATGCATCTGGTAGGGAGAAGTTTCCACACCCTTATAAAGAAGCTTCGTTCCCTTCTCCAACCGATGTGGGATCTCATGGACTATATCTACTAGCAGTAGGCTCGGGCTCTTATTGTGAACGAAAGTGAATATCAAACCGCTTTCTCCTTTCAGAATGCTGTTTTTACCACTTGAAACAGGGAGATAGCCAACAATATGAACTCCCAGTGAAAGAGAAAAGTGATATCTGCACTATCATGTACACCAGTGGAACAACCGGTGATCCCAAGGGAGTACTGTTATCCAATAATGCCATTATAGCACTTATAGCCGGCGTCAAACATTTGCTTACATCTTTAAATGTAGAGGTGATCTCAGTTCTTCCCCTTCATAACTCATTTTTCAAGCTTGTTGATTCAAATGAGATGCGTACGTGTTCATCGTTCTTGTTATAGGTTCAAGAAAATGATGTTTACATATCTTATCTTCCGCTTGCTCATATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTAAACGGTGGCTCCATCGGATTCTGGCGTGGGGTGAGAAATCATATATTGATCTTTCCCTTTTCAAAAGGCAAATAAATTGAAGAACTCATATCATGTCATGTCCATGAATTTTGTAGGATGTGAAATTGTTAGTTGAAGACATTGGCGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTTCTTGAAAGAATCTATGGTGGTGAGCGTATAAATTTGTTGCTTTCAAATTCGATATCGTGGATTCAAACCCCTGCAATGTCGTTTTGACATTCGGGCTAATACTTAAGCCAACACGTAAAAAAAATATTATATTTATACGGATCTATCAACTTAAACGAATATGTTAACTTTTACCAATGTGTTTGATGACGTTGTTTAGCTTTGAACCAGAAGATATCTTCAGGAAGCTTGATAAAAAAATCGATGTTTAACTTTGCATACTCGTAGTAAGTGAAAATTTCTTATCGAACTCTCGACTTGTTCATATTCGAGTTCCTGTTGTTTTAAATTCTTCCATGCTGTTCTATCTCTGCAGCAAATATAACCAAATGCAGAAGGGGAAAAAACATGAAGTAGCAGCCCCACTTTTTGATAAACTTGTGTTTGACAAGGTAAAAGGCTTCACTTCAATCAAAACCACCTTAAAAACGTCGAAAGCTCATTCATGTTCTTGCAAGGATGTTTTTAGGTGAAGAAGGGATTAGGAGGAAGAGTGCGGATCATTTTATCTGGCGGAGCGCCTCTTGCAGCTCATATCGAAACTTTCTTTCGAGTCGTGACTTGTGCTCATGTTCTACAAGGCTATGGTATTATCATCCTTACCTTAATTTACACAAAAACAAACATGTTGTGTTTAGAATTTCCAAACAAGCCTTTATTTGCAGGTTTGACAGAAACTTGTGGTGGAACATTTGTCAGTCTACCAAATGAGTTGCCAATGTTGGGAACAGTTGGCCCTCCAGTGCCTAATGTGGATATCTGTCTCGAATCAGTACCCGAACTGGGATACGATGCTCTCGCTAGCACGCCTCAGGGTGAAGTATGCATCAAGGGAGATACATTGTTTTCAGGATACCATAAACGCGAAGATCTTACCACAGAGGTCTTGGTCGATGGGTGGTTTCACACTGGTACTACCGATTATTCTTTCGAAATTTTCATCTCTCGACGGTCAAAAGCATGTCGATATCACTCGGATCCTTACATTCTTTGAGTCGTATTTAGGGGATGTTGGCGAATGGCAACCCGATGGAAGCTTGAAAATTATCGACCGCAAGAAGAACATCTTCAAACTTTCTCAAGGCGAATATGTTGCAGTGGAAAATCTCGAGCTGATTTATGGCCTTGTTTCCGACATCGAAATGGTACGAATTCTATCTCCTACTATTATTCTACAACGCCAAAAACGTCGCCGTAACTTGTATAGCATAATCGTTCATCATTAACAAAAAAAATATGGAAACCATGTTCGAAACTTTAGCGACACGGAGTATAAACGTCGTTAAGAGTTAGCTAAAATGTAGAAAGTGATAATATTTGAATGAGATTGTTCAGATTTGGATATATGGGAGTAGCTTCGAGTCCTTCCTTGTTGCTGTTGTTAACCCAAAAAAAGAAGCACTTGAACATTGGGCAGAAGAAAATGGAATCAAGGGGGATTTTGCTGATCTTTGTGGAGACAGAAGGGCAAAAGATTATATTCTTGGACAACTTTCAAAGATTGCCAAAGAAAAAAAGGTTTGTTTTTTATTTCTTCTCGTACGAAGATGAGTTGAACGAACGAAGCATGAATTGAAGTTGAAGCGTTTTCTTTTGCAGTTAAAAGGTTACGAAGCTATTCGAGCGGTTCATCTCGACCCCATCTTATTCGATATTGAGCGTGACCTTCTTACTCCATCGTTTAAGAAGAAGAGACCGCAACTGCTCAAATACTATCAGGTTCTTTCCTCTCTACAAACTTGATATCATTAACTTATGTTTGTTGTCGATGTTTCGAATGTTTTTGAATCTGAAAGTTTTAATATAAATTAACGGTCGAATTTGTGAACAGAAAGAGATCGATGACATGTACAAGAGCGGGAGCAAGCCAGTTGCGTAAGGAAGAAGATTGAAGATGGGAAGGATGTAAAACTTCTTGTTTCAATGGAAATTCAACTCTTCCTCATTTTTTTTTTTATAAATTGGTTTCCTTGGAGTTAAACTAGACATTCATCATTTTTATACCCATTAATATATATATATATATTTTTCACTTTTGCTTTA

mRNA sequence

AACATTGCGCCCACGTCGTCTCCAGATTTCAACCCTAAATAAAGTTCTCAGGTTTTGGGAATTTCTTGAAATTTCTTGAAGTTTCAGAAGAAGAACAAGAAGAACAATGCAGATGAAGTATGTGATTCAGATGGAGGAGGCCAAGGAAGCCGCCGAGGACCGCCCATCGGTGGGGGCGGTTTACCGGAGCATCTTTGCCAAAGATGGGTTTCCGCCTCCGGTTGAAGGCTTGGATACCTGCTGGGATATATTTCGAATGTCTGTGGAGAAAAATCCTGGAAATCGGATGCTTGGACGTCGAAAAATAGTTGAAGGCAATGTAGCTAATGCTGGTGAATATCAGTGGCTAACTTACAAAGAAGTCTATGACTTGGTGTTGAAAATTGGGAATGCAATTCGCAGCATGGGTTACGGACCAGGAGAAAAATGTGGGATTTATGGTGCTAATTGCTCTGAGTGGATCATTAGCATGGAGGCTTGCAATGCTCATGGGCTTTATTGTGTTCCTTTATATGATACTTTAGGTGCTGGTGCCATAGAATTTATAATCTGTCATGCAGAGATTTCCATTGCTTTTGTAGAAGAGAAGAAGATCTGTGAGCTGTTGAAAACACTCCCCAACACAGCAAAGACTCTTAAAACAATTGTGAGCTTTGGTAACGTTTCGAATGATCATAAGGAAGAAGTCGACAAGTTCGGGTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGGAGATAGCCAACAATATGAACTCCCAGTGAAAGAGAAAAGTGATATCTGCACTATCATGTACACCAGTGGAACAACCGGTGATCCCAAGGGAGTACTGTTATCCAATAATGCCATTATAGCACTTATAGCCGGCGTCAAACATTTGCTTACATCTTTAAATGTAGAGGTTCAAGAAAATGATGTTTACATATCTTATCTTCCGCTTGCTCATATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTAAACGGTGGCTCCATCGGATTCTGGCGTGGGGATGTGAAATTGTTAGTTGAAGACATTGGCGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTTCTTGAAAGAATCTATGGTGCTTTGAACCAGAAGATATCTTCAGGAAGCTTGATAAAAAAATCGATGTTTAACTTTGCATACTCGTACAAATATAACCAAATGCAGAAGGGGAAAAAACATGAAGTAGCAGCCCCACTTTTTGATAAACTTGTGTTTGACAAGGTGAAGAAGGGATTAGGAGGAAGAGTGCGGATCATTTTATCTGGCGGAGCGCCTCTTGCAGCTCATATCGAAACTTTCTTTCGAGTCGTGACTTGTGCTCATGTTCTACAAGGCTATGGTTTGACAGAAACTTGTGGTGGAACATTTGTCAGTCTACCAAATGAGTTGCCAATGTTGGGAACAGTTGGCCCTCCAGTGCCTAATGTGGATATCTGTCTCGAATCAGTACCCGAACTGGGATACGATGCTCTCGCTAGCACGCCTCAGGGTGAAGTATGCATCAAGGGAGATACATTGTTTTCAGGATACCATAAACGCGAAGATCTTACCACAGAGGTCTTGGTCGATGGGTGGTTTCACACTGGGGATGTTGGCGAATGGCAACCCGATGGAAGCTTGAAAATTATCGACCGCAAGAAGAACATCTTCAAACTTTCTCAAGGCGAATATGTTGCAGTGGAAAATCTCGAGCTGATTTATGGCCTTGTTTCCGACATCGAAATGATTTGGATATATGGGAGTAGCTTCGAGTCCTTCCTTGTTGCTGTTGTTAACCCAAAAAAAGAAGCACTTGAACATTGGGCAGAAGAAAATGGAATCAAGGGGGATTTTGCTGATCTTTGTGGAGACAGAAGGGCAAAAGATTATATTCTTGGACAACTTTCAAAGATTGCCAAAGAAAAAAAGTTAAAAGGTTACGAAGCTATTCGAGCGGTTCATCTCGACCCCATCTTATTCGATATTGAGCGTGACCTTCTTACTCCATCGTTTAAGAAGAAGAGACCGCAACTGCTCAAATACTATCAGAAAGAGATCGATGACATGTACAAGAGCGGGAGCAAGCCAGTTGCGTAAGGAAGAAGATTGAAGATGGGAAGGATGTAAAACTTCTTGTTTCAATGGAAATTCAACTCTTCCTCATTTTTTTTTTTATAAATTGGTTTCCTTGGAGTTAAACTAGACATTCATCATTTTTATACCCATTAATATATATATATATATTTTTCACTTTTGCTTTA

Coding sequence (CDS)

ATGCAGATGAAGTATGTGATTCAGATGGAGGAGGCCAAGGAAGCCGCCGAGGACCGCCCATCGGTGGGGGCGGTTTACCGGAGCATCTTTGCCAAAGATGGGTTTCCGCCTCCGGTTGAAGGCTTGGATACCTGCTGGGATATATTTCGAATGTCTGTGGAGAAAAATCCTGGAAATCGGATGCTTGGACGTCGAAAAATAGTTGAAGGCAATGTAGCTAATGCTGGTGAATATCAGTGGCTAACTTACAAAGAAGTCTATGACTTGGTGTTGAAAATTGGGAATGCAATTCGCAGCATGGGTTACGGACCAGGAGAAAAATGTGGGATTTATGGTGCTAATTGCTCTGAGTGGATCATTAGCATGGAGGCTTGCAATGCTCATGGGCTTTATTGTGTTCCTTTATATGATACTTTAGGTGCTGGTGCCATAGAATTTATAATCTGTCATGCAGAGATTTCCATTGCTTTTGTAGAAGAGAAGAAGATCTGTGAGCTGTTGAAAACACTCCCCAACACAGCAAAGACTCTTAAAACAATTGTGAGCTTTGGTAACGTTTCGAATGATCATAAGGAAGAAGTCGACAAGTTCGGGTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGGAGATAGCCAACAATATGAACTCCCAGTGAAAGAGAAAAGTGATATCTGCACTATCATGTACACCAGTGGAACAACCGGTGATCCCAAGGGAGTACTGTTATCCAATAATGCCATTATAGCACTTATAGCCGGCGTCAAACATTTGCTTACATCTTTAAATGTAGAGGTTCAAGAAAATGATGTTTACATATCTTATCTTCCGCTTGCTCATATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTAAACGGTGGCTCCATCGGATTCTGGCGTGGGGATGTGAAATTGTTAGTTGAAGACATTGGCGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTTCTTGAAAGAATCTATGGTGCTTTGAACCAGAAGATATCTTCAGGAAGCTTGATAAAAAAATCGATGTTTAACTTTGCATACTCGTACAAATATAACCAAATGCAGAAGGGGAAAAAACATGAAGTAGCAGCCCCACTTTTTGATAAACTTGTGTTTGACAAGGTGAAGAAGGGATTAGGAGGAAGAGTGCGGATCATTTTATCTGGCGGAGCGCCTCTTGCAGCTCATATCGAAACTTTCTTTCGAGTCGTGACTTGTGCTCATGTTCTACAAGGCTATGGTTTGACAGAAACTTGTGGTGGAACATTTGTCAGTCTACCAAATGAGTTGCCAATGTTGGGAACAGTTGGCCCTCCAGTGCCTAATGTGGATATCTGTCTCGAATCAGTACCCGAACTGGGATACGATGCTCTCGCTAGCACGCCTCAGGGTGAAGTATGCATCAAGGGAGATACATTGTTTTCAGGATACCATAAACGCGAAGATCTTACCACAGAGGTCTTGGTCGATGGGTGGTTTCACACTGGGGATGTTGGCGAATGGCAACCCGATGGAAGCTTGAAAATTATCGACCGCAAGAAGAACATCTTCAAACTTTCTCAAGGCGAATATGTTGCAGTGGAAAATCTCGAGCTGATTTATGGCCTTGTTTCCGACATCGAAATGATTTGGATATATGGGAGTAGCTTCGAGTCCTTCCTTGTTGCTGTTGTTAACCCAAAAAAAGAAGCACTTGAACATTGGGCAGAAGAAAATGGAATCAAGGGGGATTTTGCTGATCTTTGTGGAGACAGAAGGGCAAAAGATTATATTCTTGGACAACTTTCAAAGATTGCCAAAGAAAAAAAGTTAAAAGGTTACGAAGCTATTCGAGCGGTTCATCTCGACCCCATCTTATTCGATATTGAGCGTGACCTTCTTACTCCATCGTTTAAGAAGAAGAGACCGCAACTGCTCAAATACTATCAGAAAGAGATCGATGACATGTACAAGAGCGGGAGCAAGCCAGTTGCGTAA

Protein sequence

MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRMLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIISMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIVSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKGVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFWRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGLTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFSGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQLSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGSKPVA
Homology
BLAST of Carg08564 vs. NCBI nr
Match: KAG7035485.1 (Long chain acyl-CoA synthetase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1354.3 bits (3504), Expect = 0.0e+00
Identity = 664/664 (100.00%), Postives = 664/664 (100.00%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII
Sbjct: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG
Sbjct: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS
Sbjct: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of Carg08564 vs. NCBI nr
Match: XP_022958004.1 (long chain acyl-CoA synthetase 4-like [Cucurbita moschata])

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 663/664 (99.85%), Postives = 664/664 (100.00%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII
Sbjct: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG
Sbjct: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYGSSFESFLVAVVNPKKEA+EHWAEENGIKGDFADLCGDRRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEAVEHWAEENGIKGDFADLCGDRRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS
Sbjct: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of Carg08564 vs. NCBI nr
Match: KAG6605571.1 (Long chain acyl-CoA synthetase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 663/664 (99.85%), Postives = 663/664 (99.85%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII
Sbjct: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVV CAHVLQGYG
Sbjct: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVACAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS
Sbjct: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of Carg08564 vs. NCBI nr
Match: XP_023534399.1 (long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1340.1 bits (3467), Expect = 0.0e+00
Identity = 654/664 (98.49%), Postives = 662/664 (99.70%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQMKYVIQMEE KEAAEDRPSVGAVYRS+FAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQMKYVIQMEEPKEAAEDRPSVGAVYRSVFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGN+ANAGEY+WLTYKEVYDLVLKIGNA+RSMGYGPGEKCGIYGANCSEWII
Sbjct: 61  MLGRRKIVEGNLANAGEYEWLTYKEVYDLVLKIGNAMRSMGYGPGEKCGIYGANCSEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKI ELLKTLPNTAKTLKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKISELLKTLPNTAKTLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVV+CAHVLQGYG
Sbjct: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFAD+CGD+RAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADICGDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKI+KEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS
Sbjct: 601 LSKISKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of Carg08564 vs. NCBI nr
Match: XP_022995391.1 (long chain acyl-CoA synthetase 4-like [Cucurbita maxima])

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 649/664 (97.74%), Postives = 661/664 (99.55%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGN+ANAGEY+WLTYK+VYDLVLKIGNA+RSMGYGPGEKCGIYGANCSEWII
Sbjct: 61  MLGRRKIVEGNLANAGEYEWLTYKQVYDLVLKIGNAMRSMGYGPGEKCGIYGANCSEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKI ELLKTLPNTAKTLKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKISELLKTLPNTAKTLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFGNV+NDHK EVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGNVANDHKGEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHE+AAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVV+CAHVLQGYG
Sbjct: 361 QKGKKHELAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGY+ALASTPQGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYNALASTPQGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWI+GSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGD+RAKDYILGQ
Sbjct: 541 GLVSDIEMIWIFGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKEKKLKGYEAIR VHLDPILFDIERDLLTPSFKKKRPQLLKYY+KEIDDMYKSGS
Sbjct: 601 LSKIAKEKKLKGYEAIRVVHLDPILFDIERDLLTPSFKKKRPQLLKYYKKEIDDMYKSGS 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of Carg08564 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 8.7e-287
Identity = 458/657 (69.71%), Postives = 554/657 (84.32%), Query Frame = 0

Query: 2   QMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRM 61
           Q KY+ Q+EE KE ++ RPSVG VYRSIFAKDGFP P+EG+D+CWD+FRMSVEK P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 62  LGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIIS 121
           LGRR+IV+G     G+Y W TY+EVYD+V+K+GN++RS+G     KCGIYGAN  EWIIS
Sbjct: 64  LGRREIVDG---KPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIV 181
           MEACNAHGLYCVPLYDTLGA A+EFII H+E+SI FVEEKKI EL KT PN+ + +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVV 183

Query: 182 SFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKG 241
           SFG VS + KEE + FGL IY+W+EFL+ G+ +QY+LP+K+KSDICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFW 301
           V++SN +I+ LIAGV  LL S N  +   DVY+SYLPLAHIFDRVIEE FI +G +IGFW
Sbjct: 244 VMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQ 361
           RGDVKLL+ED+ ELKPTIFCAVPRVL+R+Y  L +K+S G  +KK +F+ A+SYK+  M+
Sbjct: 304 RGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMK 363

Query: 362 KGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGL 421
           KG+ H  A+PLFDKLVF KVK+GLGG VRIILSG APLA+H+E+F RVV C HVLQGYGL
Sbjct: 364 KGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGL 423

Query: 422 TETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFS 481
           TE+C GTFVSLP+EL MLGTVGPPVPNVDI LESVPE+ YDALAST +GE+CI+G TLFS
Sbjct: 424 TESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFS 483

Query: 482 GYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GY+KREDLT EVL+DGW HTGDVGEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+E IYG
Sbjct: 484 GYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYG 543

Query: 542 LVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQL 601
            V  ++ +W+YG+SFESFL+A+ NP +  LE WA ENG+ GD+  LC + +AK++ILG+L
Sbjct: 544 EVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGEL 603

Query: 602 SKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKS 659
            K+AKEKK+KG+E I+A+HLDP+ FD+ERDLLTP+FKKKRPQLLKYYQ  ID+MYK+
Sbjct: 604 VKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKT 657

BLAST of Carg08564 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 6.0e-280
Identity = 447/663 (67.42%), Postives = 543/663 (81.90%), Query Frame = 0

Query: 2   QMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRM 61
           Q +++ ++E AKEA +  PSVG VYRS FA++GFP P++G+ +CWDIFR +VEK P NRM
Sbjct: 4   QKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRM 63

Query: 62  LGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIIS 121
           LGRR+I  G    AG+Y W TYKEVYD+V+K+GN++RS G   GEKCGIYG NC EWIIS
Sbjct: 64  LGRREISNG---KAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIV 181
           MEACNAHGLYCVPLYDTLGAGA+EFII HAE+SIAFVEEKKI EL KT PN+ K +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVV 183

Query: 182 SFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKG 241
           SFG V  + KEE +K GL I+SW+EFL+ G+ +QYELP+K+ SDICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFW 301
           V++SN +I+ +  GV H L ++N  + E DVYISYLPLAH+FDR IEE  I  GGSIGFW
Sbjct: 244 VMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQ 361
           RGDVKLL+ED+GELKP+IFCAVPRVL+R+Y  L QK+S G   KK +F+ A+SYK+  M+
Sbjct: 304 RGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMK 363

Query: 362 KGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGL 421
           KG+ H  A+P  DKLVF+KVK+GLGG VRIILSG APLA+HIE+F RVV C +VLQGYGL
Sbjct: 364 KGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGL 423

Query: 422 TETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFS 481
           TE+C GTF + P+EL MLGTVGPPVPNVDI LESVPE+ YDAL STP+GE+CI+G TLFS
Sbjct: 424 TESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFS 483

Query: 482 GYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GY+KREDLT EV +DGW HTGDVGEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLE +Y 
Sbjct: 484 GYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYS 543

Query: 542 LVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQL 601
            V  IE IW+YG+SFESFLVA+ NP ++ LE WA ENG+ GDF  +C + +AK +ILG+L
Sbjct: 544 QVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGEL 603

Query: 602 SKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGSK 661
            K AKE KLKG+E I+ VHL+P+ FD+ERDLLTP++KKKRPQLLKYYQ  I +MYK+  +
Sbjct: 604 VKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 663

Query: 662 PVA 665
            +A
Sbjct: 664 SLA 663

BLAST of Carg08564 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 1.5e-278
Identity = 446/655 (68.09%), Postives = 549/655 (83.82%), Query Frame = 0

Query: 4   KYVIQMEEAKEAAE-DRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRML 63
           +Y++++E+ K+  +   PSVG VYRSI+AKDGFP P + L + WDIFR+SVEK+P N ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 64  GRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIISM 123
           GRR+IV+G    AG+Y W TYKEV+++V+K+GN+IR++G G G+KCGIYGAN  EWIISM
Sbjct: 65  GRREIVDG---KAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISM 124

Query: 124 EACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIVS 183
           EACNAHGLYCVPLYDTLGAGAIEFIICHAE+S+AF EE KI ELLKT P + K LK IVS
Sbjct: 125 EACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVS 184

Query: 184 FGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKGV 243
           FG V+N+ + E ++  L IYSW++FL+ G+ + YELP K +SD+CTIMYTSGTTGDPKGV
Sbjct: 185 FGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGV 244

Query: 244 LLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFWR 303
           LL+N +II L+ GVK LL +++ E+   DVY+SYLPLAHIFDRVIEE+ I    SIGFWR
Sbjct: 245 LLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWR 304

Query: 304 GDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQK 363
           GDVK+L+EDI  LKPT+FCAVPRVLERIY  L QK+S G  +KK +FNFA+ YK+  M+K
Sbjct: 305 GDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEK 364

Query: 364 GKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGLT 423
           G+ HE A+P+ DK+VF KVK+GLGG VR+ILSG APLAAHIE+F RVV CAHVLQGYGLT
Sbjct: 365 GQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLT 424

Query: 424 ETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFSG 483
           E+CGGTFVS+PNEL MLGTVGPPVPNVDI LESVPE+GYDALAS P+GE+CI+G TLFSG
Sbjct: 425 ESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSG 484

Query: 484 YHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGL 543
           Y+KREDLT EV +DGW HTGDVGEWQPDG++KIIDRKKNIFKLSQGEYVAVENLE IY  
Sbjct: 485 YYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSH 544

Query: 544 VSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQLS 603
           V+ IE IW+YG+S+ES+LVAVV P K  +EHWA+E+ + GDF  +C +++ K+++LG+ +
Sbjct: 545 VAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFN 604

Query: 604 KIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYK 658
           ++AK+KKLKG+E I+ VHLD + FD+ERDL+TPS+K KRPQLLKYYQKEID+MYK
Sbjct: 605 RVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656

BLAST of Carg08564 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 3.6e-224
Identity = 361/659 (54.78%), Postives = 485/659 (73.60%), Query Frame = 0

Query: 3   MKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRML 62
           M Y +++EEA+ A E  PS G VYRSI+A+DG     EGL + W+    SV+++P   ML
Sbjct: 1   MSYTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPML 60

Query: 63  GRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIISM 122
           GRR+I +   + AG Y WLTY+EV+D  +++ +A+RS G  PG++CGIYG NC +WI++M
Sbjct: 61  GRRQIKD---SEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAM 120

Query: 123 EACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIVS 182
           +AC +H +  VPLYDTLG  A+EFII H E+SIAFV+E KI  +L  LPN +  LKTIVS
Sbjct: 121 QACYSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVS 180

Query: 183 FGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKGV 242
           FGN+++  K+E +  G+  +SWEEF Q G+    ELP K ++DICT+MYTSG TG+PKGV
Sbjct: 181 FGNITSVQKKETEALGVSCFSWEEFSQLGNLSG-ELPEKHRTDICTLMYTSGATGEPKGV 240

Query: 243 LLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFWR 302
           +L+N AI+A I    ++L   +    E DVY SYLPLAH++D+++E   I  G +IG+WR
Sbjct: 241 ILTNEAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWR 300

Query: 303 GDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQK 362
           GD++ L++D+ ELKPTIFC VPRV +RIY  +  K+SSG  +KK +F +AY+YK   M+K
Sbjct: 301 GDIRFLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEK 360

Query: 363 GKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGLT 422
           G  H  AAPL DKL FDK+K+G GGR+R++ SG APL  H+E + RV +CA + QGYGLT
Sbjct: 361 GLPHGQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLT 420

Query: 423 ETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFSG 482
           E+CGG   S+ N  PM+GTVG P+  ++  LESVPE+GYDAL+  P+GE+C++G TLFSG
Sbjct: 421 ESCGGCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSG 480

Query: 483 YHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGL 542
           YHKREDLT +VLVDGWFHTGD+GEWQP+G++KIIDRKKNIFKLSQGEYV VEN+E IY  
Sbjct: 481 YHKREDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQ 540

Query: 543 VSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQLS 602
              I  IW+YG+SFESFLVAVV P++ ALE+WA    +  DF  LC + +A  YIL +L+
Sbjct: 541 CPLIASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELN 600

Query: 603 KIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGSK 662
             AK+ +L+G+E ++AV+L+P  FD+ERDL+TP+FK KRPQLLKYY+  +D +Y    +
Sbjct: 601 STAKKHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLYSEAKE 655

BLAST of Carg08564 vs. ExPASy Swiss-Prot
Match: Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.5e-209
Identity = 342/661 (51.74%), Postives = 467/661 (70.65%), Query Frame = 0

Query: 6   VIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRMLGRR 65
           V+ +EE + A  + PS G VYR  +AKDG       +D+ W  F  +V+K P  +MLG+R
Sbjct: 8   VLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQR 67

Query: 66  KIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIISMEAC 125
              +  V   G Y W+TYKE +D  ++IG+AIRS G  PG  CGIYGANC EWII+MEAC
Sbjct: 68  VTTDSKV---GPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEAC 127

Query: 126 NAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIVSFGN 185
            + G+  VPLYD+LG  A+EFII HAE+S+ FV+EK +  +L      +  LKTIVSFG 
Sbjct: 128 MSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGE 187

Query: 186 VSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKGVLLS 245
           VS+  KEE     + ++SW EF   G+  +  LP K K+DICTIMYTSGTTG+PKGV+L+
Sbjct: 188 VSSTQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILN 247

Query: 246 NNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFWRGDV 305
           N AI   +  +  +L   +     +DV+ SYLPLAH +D+V+E  F+  G S+G+WRGD+
Sbjct: 248 NAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDI 307

Query: 306 KLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQKGKK 365
           + L++D+  LKPT+FC VPRV +++Y  + QKIS+  LI+K +F+FAY+YK   M+KG  
Sbjct: 308 RYLMDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFS 367

Query: 366 HEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGLTETC 425
            E A+P  D+L+FDK+K+ LGGR  ++LSG APL  H+E F R++  +++ QGYGLTE+C
Sbjct: 368 QEEASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESC 427

Query: 426 GGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDAL-ASTPQGEVCIKGDTLFSGYH 485
           GG+F +L     M+GTVG P+P V+  L SVPE+GYDA  A  P+GE+C++G+++FSGYH
Sbjct: 428 GGSFTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYH 487

Query: 486 KREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVS 545
           KR+DLT +VL+DGWFHTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLE  Y    
Sbjct: 488 KRQDLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCP 547

Query: 546 DIEMIWIYGSSFESFLVAVVNPKKEALEHWAEEN-GIKGDFADLCGDRRAKDYILGQLSK 605
            I  IW+YG+SFESFLV VV P ++A+E WA+ N     DF  LC + +A+ Y L +L+ 
Sbjct: 548 LIAQIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNS 607

Query: 606 IAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGSKPV 665
            AK+ +LKG+E ++A+HL+P  FDIERDL+TP+FK KRPQLL++Y+  +D +Y    + +
Sbjct: 608 TAKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKRSM 665

BLAST of Carg08564 vs. ExPASy TrEMBL
Match: A0A6J1H3R4 (long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111459367 PE=4 SV=1)

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 663/664 (99.85%), Postives = 664/664 (100.00%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII
Sbjct: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG
Sbjct: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYGSSFESFLVAVVNPKKEA+EHWAEENGIKGDFADLCGDRRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEAVEHWAEENGIKGDFADLCGDRRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS
Sbjct: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of Carg08564 vs. ExPASy TrEMBL
Match: A0A6J1K1T5 (long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111490951 PE=4 SV=1)

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 649/664 (97.74%), Postives = 661/664 (99.55%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGN+ANAGEY+WLTYK+VYDLVLKIGNA+RSMGYGPGEKCGIYGANCSEWII
Sbjct: 61  MLGRRKIVEGNLANAGEYEWLTYKQVYDLVLKIGNAMRSMGYGPGEKCGIYGANCSEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKI ELLKTLPNTAKTLKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKISELLKTLPNTAKTLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFGNV+NDHK EVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGNVANDHKGEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHE+AAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVV+CAHVLQGYG
Sbjct: 361 QKGKKHELAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGY+ALASTPQGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYNALASTPQGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWI+GSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGD+RAKDYILGQ
Sbjct: 541 GLVSDIEMIWIFGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKEKKLKGYEAIR VHLDPILFDIERDLLTPSFKKKRPQLLKYY+KEIDDMYKSGS
Sbjct: 601 LSKIAKEKKLKGYEAIRVVHLDPILFDIERDLLTPSFKKKRPQLLKYYKKEIDDMYKSGS 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of Carg08564 vs. ExPASy TrEMBL
Match: A0A5D3C2P4 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001880 PE=4 SV=1)

HSP 1 Score: 1234.9 bits (3194), Expect = 0.0e+00
Identity = 596/664 (89.76%), Postives = 633/664 (95.33%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQ KY++Q+EEAK+A   RPSVG VYRSIFAKDGFPPPV+GLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRK+VEGN A AGEY+WLTYKEVY+LVLKIGNA+R +GYGPGEKCGIYGANC EWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAEISIAFVEEKKI ELLKTLPNTAK LKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFG VS++HKEEV KFGLEIY+WEEFLQKGDSQQ++LPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLT++N  VQENDVYISYLPLAHIFDRVIEEVFILNG SIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYG LNQKISSG  +K+S+FN AYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKH+ AAPLFDK+VFDKVKKGLGG VRIILSGGAPLAAHIETFFRVV+CAHVLQGYG
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFV+LP+ELPMLGTVGPPVPNVDICLESVPE+GYD+ ASTP+GEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGY+KR+DLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYG+SFESFLVAVVNPKK+ALE WAEENGIK DF  LC D+RAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKEDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKE+KLKGYE IRAVHLDP+ FDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSG+
Sbjct: 601 LSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of Carg08564 vs. ExPASy TrEMBL
Match: A0A1S3AUH9 (long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103482821 PE=4 SV=1)

HSP 1 Score: 1234.9 bits (3194), Expect = 0.0e+00
Identity = 596/664 (89.76%), Postives = 633/664 (95.33%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQ KY++Q+EEAK+A   RPSVG VYRSIFAKDGFPPPV+GLDTCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRK+VEGN A AGEY+WLTYKEVY+LVLKIGNA+R +GYGPGEKCGIYGANC EWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAEISIAFVEEKKI ELLKTLPNTAK LKTI
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFG VS++HKEEV KFGLEIY+WEEFLQKGDSQQ++LPVKEKSDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSNNAIIALIAGVKHLLT++N  VQENDVYISYLPLAHIFDRVIEEVFILNG SIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYG LNQKISSG  +K+S+FN AYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKH+ AAPLFDK+VFDKVKKGLGG VRIILSGGAPLAAHIETFFRVV+CAHVLQGYG
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFV+LP+ELPMLGTVGPPVPNVDICLESVPE+GYD+ ASTP+GEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGY+KR+DLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYG+SFESFLVAVVNPKK+ALE WAEENGIK DF  LC D+RAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKEDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKE+KLKGYE IRAVHLDP+ FDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSG+
Sbjct: 601 LSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of Carg08564 vs. ExPASy TrEMBL
Match: A0A6J1ER85 (long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435809 PE=4 SV=1)

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 591/664 (89.01%), Postives = 629/664 (94.73%), Query Frame = 0

Query: 1   MQMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNR 60
           MQ KY+ Q+EE KEAA  RPSVG VYRS+FAKDGFPP VEGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLFQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVEGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWII 120
           MLGRRKIVEGN A AGEY+WLTY++VY+LVLKIGNA+RS+GYGPGEKCGIYGANC EWII
Sbjct: 61  MLGRRKIVEGNPAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTI 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAE SIAFVEEKKI ELLKTLPNTA  LKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPK 240
           VSFG VSN+HKEEV+KFGLEIYSWEEFLQKGDSQQY+ PVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDPPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGF 300
           GVLLSN AIIALIAGVKHLL ++  EVQENDVYISYLPLAHIFDRVIEEVFILNG SIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYG LNQKISSG L+K+S+FNFAYSYKYNQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 QKGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYG 420
           QKGKKHE AA LFDKLVFDKVKKGLGG+VRIILSG APLAAHIETFFRVV+CAHVLQGYG
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLF 480
           LTETCGGTFV+LPNEL MLGTVGPPVPNVDICLESVPE+GYD+LASTP+GEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 SGYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           +GY+KREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQ 600
           GLVSDIEMIWIYG+SFE+FLVAVVNP+++ALEHWAEENGIKGDF  LC D+RAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFEAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGS 660
           LSKIAKEKKLKGYE I+AVHLDP+ FDIERDLLTPSFKKKRPQ+LKYYQKEID+MYK G+
Sbjct: 601 LSKIAKEKKLKGYEVIKAVHLDPLPFDIERDLLTPSFKKKRPQMLKYYQKEIDEMYKGGN 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of Carg08564 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 987.3 bits (2551), Expect = 6.2e-288
Identity = 458/657 (69.71%), Postives = 554/657 (84.32%), Query Frame = 0

Query: 2   QMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRM 61
           Q KY+ Q+EE KE ++ RPSVG VYRSIFAKDGFP P+EG+D+CWD+FRMSVEK P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 62  LGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIIS 121
           LGRR+IV+G     G+Y W TY+EVYD+V+K+GN++RS+G     KCGIYGAN  EWIIS
Sbjct: 64  LGRREIVDG---KPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIV 181
           MEACNAHGLYCVPLYDTLGA A+EFII H+E+SI FVEEKKI EL KT PN+ + +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVV 183

Query: 182 SFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKG 241
           SFG VS + KEE + FGL IY+W+EFL+ G+ +QY+LP+K+KSDICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFW 301
           V++SN +I+ LIAGV  LL S N  +   DVY+SYLPLAHIFDRVIEE FI +G +IGFW
Sbjct: 244 VMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQ 361
           RGDVKLL+ED+ ELKPTIFCAVPRVL+R+Y  L +K+S G  +KK +F+ A+SYK+  M+
Sbjct: 304 RGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMK 363

Query: 362 KGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGL 421
           KG+ H  A+PLFDKLVF KVK+GLGG VRIILSG APLA+H+E+F RVV C HVLQGYGL
Sbjct: 364 KGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGL 423

Query: 422 TETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFS 481
           TE+C GTFVSLP+EL MLGTVGPPVPNVDI LESVPE+ YDALAST +GE+CI+G TLFS
Sbjct: 424 TESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFS 483

Query: 482 GYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GY+KREDLT EVL+DGW HTGDVGEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+E IYG
Sbjct: 484 GYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYG 543

Query: 542 LVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQL 601
            V  ++ +W+YG+SFESFL+A+ NP +  LE WA ENG+ GD+  LC + +AK++ILG+L
Sbjct: 544 EVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGEL 603

Query: 602 SKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKS 659
            K+AKEKK+KG+E I+A+HLDP+ FD+ERDLLTP+FKKKRPQLLKYYQ  ID+MYK+
Sbjct: 604 VKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKT 657

BLAST of Carg08564 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 964.5 bits (2492), Expect = 4.3e-281
Identity = 447/663 (67.42%), Postives = 543/663 (81.90%), Query Frame = 0

Query: 2   QMKYVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRM 61
           Q +++ ++E AKEA +  PSVG VYRS FA++GFP P++G+ +CWDIFR +VEK P NRM
Sbjct: 4   QKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRM 63

Query: 62  LGRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIIS 121
           LGRR+I  G    AG+Y W TYKEVYD+V+K+GN++RS G   GEKCGIYG NC EWIIS
Sbjct: 64  LGRREISNG---KAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIV 181
           MEACNAHGLYCVPLYDTLGAGA+EFII HAE+SIAFVEEKKI EL KT PN+ K +KT+V
Sbjct: 124 MEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVV 183

Query: 182 SFGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKG 241
           SFG V  + KEE +K GL I+SW+EFL+ G+ +QYELP+K+ SDICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFW 301
           V++SN +I+ +  GV H L ++N  + E DVYISYLPLAH+FDR IEE  I  GGSIGFW
Sbjct: 244 VMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQ 361
           RGDVKLL+ED+GELKP+IFCAVPRVL+R+Y  L QK+S G   KK +F+ A+SYK+  M+
Sbjct: 304 RGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMK 363

Query: 362 KGKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGL 421
           KG+ H  A+P  DKLVF+KVK+GLGG VRIILSG APLA+HIE+F RVV C +VLQGYGL
Sbjct: 364 KGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGL 423

Query: 422 TETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFS 481
           TE+C GTF + P+EL MLGTVGPPVPNVDI LESVPE+ YDAL STP+GE+CI+G TLFS
Sbjct: 424 TESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFS 483

Query: 482 GYHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GY+KREDLT EV +DGW HTGDVGEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLE +Y 
Sbjct: 484 GYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYS 543

Query: 542 LVSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQL 601
            V  IE IW+YG+SFESFLVA+ NP ++ LE WA ENG+ GDF  +C + +AK +ILG+L
Sbjct: 544 QVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGEL 603

Query: 602 SKIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGSK 661
            K AKE KLKG+E I+ VHL+P+ FD+ERDLLTP++KKKRPQLLKYYQ  I +MYK+  +
Sbjct: 604 VKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 663

Query: 662 PVA 665
            +A
Sbjct: 664 SLA 663

BLAST of Carg08564 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 959.9 bits (2480), Expect = 1.1e-279
Identity = 446/655 (68.09%), Postives = 549/655 (83.82%), Query Frame = 0

Query: 4   KYVIQMEEAKEAAE-DRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRML 63
           +Y++++E+ K+  +   PSVG VYRSI+AKDGFP P + L + WDIFR+SVEK+P N ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 64  GRRKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIISM 123
           GRR+IV+G    AG+Y W TYKEV+++V+K+GN+IR++G G G+KCGIYGAN  EWIISM
Sbjct: 65  GRREIVDG---KAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISM 124

Query: 124 EACNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIVS 183
           EACNAHGLYCVPLYDTLGAGAIEFIICHAE+S+AF EE KI ELLKT P + K LK IVS
Sbjct: 125 EACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVS 184

Query: 184 FGNVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKGV 243
           FG V+N+ + E ++  L IYSW++FL+ G+ + YELP K +SD+CTIMYTSGTTGDPKGV
Sbjct: 185 FGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGV 244

Query: 244 LLSNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFWR 303
           LL+N +II L+ GVK LL +++ E+   DVY+SYLPLAHIFDRVIEE+ I    SIGFWR
Sbjct: 245 LLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWR 304

Query: 304 GDVKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQK 363
           GDVK+L+EDI  LKPT+FCAVPRVLERIY  L QK+S G  +KK +FNFA+ YK+  M+K
Sbjct: 305 GDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEK 364

Query: 364 GKKHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGLT 423
           G+ HE A+P+ DK+VF KVK+GLGG VR+ILSG APLAAHIE+F RVV CAHVLQGYGLT
Sbjct: 365 GQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLT 424

Query: 424 ETCGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFSG 483
           E+CGGTFVS+PNEL MLGTVGPPVPNVDI LESVPE+GYDALAS P+GE+CI+G TLFSG
Sbjct: 425 ESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSG 484

Query: 484 YHKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGL 543
           Y+KREDLT EV +DGW HTGDVGEWQPDG++KIIDRKKNIFKLSQGEYVAVENLE IY  
Sbjct: 485 YYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSH 544

Query: 544 VSDIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQLS 603
           V+ IE IW+YG+S+ES+LVAVV P K  +EHWA+E+ + GDF  +C +++ K+++LG+ +
Sbjct: 545 VAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFN 604

Query: 604 KIAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYK 658
           ++AK+KKLKG+E I+ VHLD + FD+ERDL+TPS+K KRPQLLKYYQKEID+MYK
Sbjct: 605 RVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656

BLAST of Carg08564 vs. TAIR 10
Match: AT1G49430.1 (long-chain acyl-CoA synthetase 2 )

HSP 1 Score: 729.9 bits (1883), Expect = 1.8e-210
Identity = 342/661 (51.74%), Postives = 467/661 (70.65%), Query Frame = 0

Query: 6   VIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRMLGRR 65
           V+ +EE + A  + PS G VYR  +AKDG       +D+ W  F  +V+K P  +MLG+R
Sbjct: 8   VLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQR 67

Query: 66  KIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIISMEAC 125
              +  V   G Y W+TYKE +D  ++IG+AIRS G  PG  CGIYGANC EWII+MEAC
Sbjct: 68  VTTDSKV---GPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEAC 127

Query: 126 NAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIVSFGN 185
            + G+  VPLYD+LG  A+EFII HAE+S+ FV+EK +  +L      +  LKTIVSFG 
Sbjct: 128 MSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGE 187

Query: 186 VSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKGVLLS 245
           VS+  KEE     + ++SW EF   G+  +  LP K K+DICTIMYTSGTTG+PKGV+L+
Sbjct: 188 VSSTQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILN 247

Query: 246 NNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFWRGDV 305
           N AI   +  +  +L   +     +DV+ SYLPLAH +D+V+E  F+  G S+G+WRGD+
Sbjct: 248 NAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDI 307

Query: 306 KLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQKGKK 365
           + L++D+  LKPT+FC VPRV +++Y  + QKIS+  LI+K +F+FAY+YK   M+KG  
Sbjct: 308 RYLMDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFS 367

Query: 366 HEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGLTETC 425
            E A+P  D+L+FDK+K+ LGGR  ++LSG APL  H+E F R++  +++ QGYGLTE+C
Sbjct: 368 QEEASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESC 427

Query: 426 GGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDAL-ASTPQGEVCIKGDTLFSGYH 485
           GG+F +L     M+GTVG P+P V+  L SVPE+GYDA  A  P+GE+C++G+++FSGYH
Sbjct: 428 GGSFTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYH 487

Query: 486 KREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVS 545
           KR+DLT +VL+DGWFHTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLE  Y    
Sbjct: 488 KRQDLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCP 547

Query: 546 DIEMIWIYGSSFESFLVAVVNPKKEALEHWAEEN-GIKGDFADLCGDRRAKDYILGQLSK 605
            I  IW+YG+SFESFLV VV P ++A+E WA+ N     DF  LC + +A+ Y L +L+ 
Sbjct: 548 LIAQIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNS 607

Query: 606 IAKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGSKPV 665
            AK+ +LKG+E ++A+HL+P  FDIERDL+TP+FK KRPQLL++Y+  +D +Y    + +
Sbjct: 608 TAKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKRSM 665

BLAST of Carg08564 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 654.8 bits (1688), Expect = 7.3e-188
Identity = 316/653 (48.39%), Postives = 442/653 (67.69%), Query Frame = 0

Query: 5   YVIQMEEAKEAAEDRPSVGAVYRSIFAKDGFPPPVEGLDTCWDIFRMSVEKNPGNRMLGR 64
           +  ++EE  +  + +PSVG VYR++ ++ GFPP    + T WDIF  SVEK P N MLG 
Sbjct: 4   FAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNMLGW 63

Query: 65  RKIVEGNVANAGEYQWLTYKEVYDLVLKIGNAIRSMGYGPGEKCGIYGANCSEWIISMEA 124
           R+IV+  V   G Y W TYKEVY+ VL+IG+A+R+ G  PG + GIYG NC +WII+MEA
Sbjct: 64  RRIVDEKV---GPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 123

Query: 125 CNAHGLYCVPLYDTLGAGAIEFIICHAEISIAFVEEKKICELLKTLPNTAKTLKTIVSFG 184
           C AH L CVPLYDTLG+GA+++I+ HAEI   FV++ KI  LL+     AK LK IVSF 
Sbjct: 124 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 183

Query: 185 NVSNDHKEEVDKFGLEIYSWEEFLQKGDSQQYELPVKEKSDICTIMYTSGTTGDPKGVLL 244
           NVS++   +  + G++ YSW +FL  G  +  +    +  +ICTIMYTSGT+GDPKGV+L
Sbjct: 184 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 243

Query: 245 SNNAIIALIAGVKHLLTSLNVEVQENDVYISYLPLAHIFDRVIEEVFILNGGSIGFWRGD 304
           ++ A+   + G+   +     ++  +DVY+S+LPLAHI DR+ EE F   G S+G++ G+
Sbjct: 244 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 303

Query: 305 VKLLVEDIGELKPTIFCAVPRVLERIYGALNQKISSGSLIKKSMFNFAYSYKYNQMQKGK 364
           + +L +DI ELKPT    VPRV ERI+  + + +   +  ++ +FN  Y +K   + +G 
Sbjct: 304 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 363

Query: 365 KHEVAAPLFDKLVFDKVKKGLGGRVRIILSGGAPLAAHIETFFRVVTCAHVLQGYGLTET 424
            H  A+P+ D + F K++  LGGR+R+++SGGAPL+  IE F RV  C  V+QGYGLTET
Sbjct: 364 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 423

Query: 425 CGGTFVSLPNELPMLGTVGPPVPNVDICLESVPELGYDALASTPQGEVCIKGDTLFSGYH 484
            GGT +  P+E+ MLGTVG P    +I LE V E+GYD L   P GE+CI+G  +FSGY+
Sbjct: 424 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 483

Query: 485 KREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVS 544
           K  +LT EV+ DGWFHTGD+GE  P+G LKIIDRKKN+ KLSQGEYVA+E+LE I+G  S
Sbjct: 484 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 543

Query: 545 DIEMIWIYGSSFESFLVAVVNPKKEALEHWAEENGIKGDFADLCGDRRAKDYILGQLSKI 604
            ++ IW+YG SF+S LVAVV P  E +  WA++ G    F +LC     K++I+ +L   
Sbjct: 544 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 603

Query: 605 AKEKKLKGYEAIRAVHLDPILFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYK 658
           A++ KL+ +E I+AV ++   FD+ERDL+T + K +R  LLKYYQ +ID+MY+
Sbjct: 604 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYR 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035485.10.0e+00100.00Long chain acyl-CoA synthetase 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022958004.10.0e+0099.85long chain acyl-CoA synthetase 4-like [Cucurbita moschata][more]
KAG6605571.10.0e+0099.85Long chain acyl-CoA synthetase 4, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023534399.10.0e+0098.49long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo][more]
XP_022995391.10.0e+0097.74long chain acyl-CoA synthetase 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9T0A08.7e-28769.71Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... [more]
Q9T0096.0e-28067.42Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... [more]
Q9C7W41.5e-27868.09Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... [more]
M4ISH13.6e-22454.78Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1[more]
Q9XIA92.5e-20951.74Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1H3R40.0e+0099.85long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111459... [more]
A0A6J1K1T50.0e+0097.74long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC11149095... [more]
A0A5D3C2P40.0e+0089.76Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AUH90.0e+0089.76long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103482821 PE... [more]
A0A6J1ER850.0e+0089.01long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435... [more]
Match NameE-valueIdentityDescription
AT4G23850.16.2e-28869.71AMP-dependent synthetase and ligase family protein [more]
AT4G11030.14.3e-28167.42AMP-dependent synthetase and ligase family protein [more]
AT1G64400.11.1e-27968.09AMP-dependent synthetase and ligase family protein [more]
AT1G49430.11.8e-21051.74long-chain acyl-CoA synthetase 2 [more]
AT2G47240.17.3e-18848.39AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 69..526
e-value: 1.4E-101
score: 340.2
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 42..518
e-value: 4.7E-89
score: 300.8
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 4..659
NoneNo IPR availablePANTHERPTHR43272:SF81LONG CHAIN ACYL-COA SYNTHETASE 4-LIKEcoord: 4..659
NoneNo IPR availableCDDcd05927LC-FACS_eukcoord: 76..657
e-value: 0.0
score: 794.882
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 41..657
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 229..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08564-RACarg08564-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0003881 CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity