Carg08521 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08521
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionmetal tolerance protein 1-like
LocationCarg_Chr02: 5198233 .. 5199498 (+)
RNA-Seq ExpressionCarg08521
SyntenyCarg08521
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGTGCAAAATCATGATCATGGTCACATGATTGAAGTAAGTGGAGATGTGCAAGCAGTAGGGCCTAGCATTGTGGGAAGTAAAATTTGTGGGGAGGCACCTTCCTGTGGATTTAAAGATGCAAAAAACAGTTCGATTGATATGAGGGAGCGAAATGCATCCATGCGGAAGCTTTTCATAGCAGCTGTTTGCTGCATCGTTTTTATGAGTATAGAAGTAGTGGGAGGTATTAAAGCAAACAGTCTTGCAATATTGACTGATGCTGCTCATTTGTTGTCGGATGTTGCTGCTTTTGCCATTTCCTTGTTCTCTCTCTGGGCCTCTGGTTGGGAGGCAACTCCGCGCCAGTCATATGGGTTCTTCCGGATTGAAATACTTGGTGCCCTCGTTTCCATTCAGATGATCTGGCTTCTTGCTGGGATTCTTGTTTATGAAGCCATTGTCCGCCTTATCAATGGTCCGGGAGAAGTCAAGGGTCTGCTCATGTTTGCTGTTTCTGCATTTGGGTTGGTTGTTAACATAGCTATGGCTCTGCTGCTCGGTCACGAACATGGTCATGCTCATTCTCATGGTCACAGTCATGGACATGGTGATCACGACCACGGTCATGGCAGCCACGAACATGGTCATGAAGATCATAGGCATCACCATGGGATTAGTGTTACCGCACACCACCACCACCACCCCCACCATGAAGAAGAGGGAGCAGCCGGCGATGGTGTTAAGCATCATCATCATCATCATCACCACGCACATGAGGAATCAGTAACTACACCCTTACTTGAAAGCCATTCACAAAAGGTTACCAAGACACAGAAAAGAGAACGAAACATAAACGTCCAGGGGGCTTACCTTCACGTACTCGGAGATTCCATCCAAAGCATCGGAGTGATGATTGGCGGGGCAATCATATGGTATAAACCCGAATACAAGATTCTTGATCTGATATGCACTCTCATTTTCTCTGTAATTGTGCTATACACAACAATTCAAATGCTCCGAAACATCCTCGAGGTTCTAATGGAGAGTACACCTCGAGAGATTGATGCAACGAAGCTCGAGAAGGGTCTATGCGAGATGGAGGAAGTAGTTGCAGTTCATGAACTGCACATCTGGGCGATAACGGTCGGAAAGGTTCTATTGGCATGCCATGTTATAATAAAGCCAGAGGCAAATGCAGATATGGTATTGGACAATGTTATAGAGTATATCAAAAGAGAGTACAACATCAGCCATGTCACCATTCAGATAGAGCGCCAGTAG

mRNA sequence

ATGGAAGTGCAAAATCATGATCATGGTCACATGATTGAAGTAAGTGGAGATGTGCAAGCAGTAGGGCCTAGCATTGTGGGAAGTAAAATTTGTGGGGAGGCACCTTCCTGTGGATTTAAAGATGCAAAAAACAGTTCGATTGATATGAGGGAGCGAAATGCATCCATGCGGAAGCTTTTCATAGCAGCTGTTTGCTGCATCGTTTTTATGAGTATAGAAGTAGTGGGAGGTATTAAAGCAAACAGTCTTGCAATATTGACTGATGCTGCTCATTTGTTGTCGGATGTTGCTGCTTTTGCCATTTCCTTGTTCTCTCTCTGGGCCTCTGGTTGGGAGGCAACTCCGCGCCAGTCATATGGGTTCTTCCGGATTGAAATACTTGGTGCCCTCGTTTCCATTCAGATGATCTGGCTTCTTGCTGGGATTCTTGTTTATGAAGCCATTGTCCGCCTTATCAATGGTCCGGGAGAAGTCAAGGGTCTGCTCATGTTTGCTGTTTCTGCATTTGGGTTGGTTGTTAACATAGCTATGGCTCTGCTGCTCGGTCACGAACATGGTCATGCTCATTCTCATGGTCACAGTCATGGACATGGTGATCACGACCACGGTCATGGCAGCCACGAACATGGTCATGAAGATCATAGGCATCACCATGGGATTAGTGTTACCGCACACCACCACCACCACCCCCACCATGAAGAAGAGGGAGCAGCCGGCGATGGTGTTAAGCATCATCATCATCATCATCACCACGCACATGAGGAATCAGTAACTACACCCTTACTTGAAAGCCATTCACAAAAGGTTACCAAGACACAGAAAAGAGAACGAAACATAAACGTCCAGGGGGCTTACCTTCACGTACTCGGAGATTCCATCCAAAGCATCGGAGTGATGATTGGCGGGGCAATCATATGGTATAAACCCGAATACAAGATTCTTGATCTGATATGCACTCTCATTTTCTCTGTAATTGTGCTATACACAACAATTCAAATGCTCCGAAACATCCTCGAGGTTCTAATGGAGAGTACACCTCGAGAGATTGATGCAACGAAGCTCGAGAAGGGTCTATGCGAGATGGAGGAAGTAGTTGCAGTTCATGAACTGCACATCTGGGCGATAACGGTCGGAAAGGTTCTATTGGCATGCCATGTTATAATAAAGCCAGAGGCAAATGCAGATATGGTATTGGACAATGTTATAGAGTATATCAAAAGAGAGTACAACATCAGCCATGTCACCATTCAGATAGAGCGCCAGTAG

Coding sequence (CDS)

ATGGAAGTGCAAAATCATGATCATGGTCACATGATTGAAGTAAGTGGAGATGTGCAAGCAGTAGGGCCTAGCATTGTGGGAAGTAAAATTTGTGGGGAGGCACCTTCCTGTGGATTTAAAGATGCAAAAAACAGTTCGATTGATATGAGGGAGCGAAATGCATCCATGCGGAAGCTTTTCATAGCAGCTGTTTGCTGCATCGTTTTTATGAGTATAGAAGTAGTGGGAGGTATTAAAGCAAACAGTCTTGCAATATTGACTGATGCTGCTCATTTGTTGTCGGATGTTGCTGCTTTTGCCATTTCCTTGTTCTCTCTCTGGGCCTCTGGTTGGGAGGCAACTCCGCGCCAGTCATATGGGTTCTTCCGGATTGAAATACTTGGTGCCCTCGTTTCCATTCAGATGATCTGGCTTCTTGCTGGGATTCTTGTTTATGAAGCCATTGTCCGCCTTATCAATGGTCCGGGAGAAGTCAAGGGTCTGCTCATGTTTGCTGTTTCTGCATTTGGGTTGGTTGTTAACATAGCTATGGCTCTGCTGCTCGGTCACGAACATGGTCATGCTCATTCTCATGGTCACAGTCATGGACATGGTGATCACGACCACGGTCATGGCAGCCACGAACATGGTCATGAAGATCATAGGCATCACCATGGGATTAGTGTTACCGCACACCACCACCACCACCCCCACCATGAAGAAGAGGGAGCAGCCGGCGATGGTGTTAAGCATCATCATCATCATCATCACCACGCACATGAGGAATCAGTAACTACACCCTTACTTGAAAGCCATTCACAAAAGGTTACCAAGACACAGAAAAGAGAACGAAACATAAACGTCCAGGGGGCTTACCTTCACGTACTCGGAGATTCCATCCAAAGCATCGGAGTGATGATTGGCGGGGCAATCATATGGTATAAACCCGAATACAAGATTCTTGATCTGATATGCACTCTCATTTTCTCTGTAATTGTGCTATACACAACAATTCAAATGCTCCGAAACATCCTCGAGGTTCTAATGGAGAGTACACCTCGAGAGATTGATGCAACGAAGCTCGAGAAGGGTCTATGCGAGATGGAGGAAGTAGTTGCAGTTCATGAACTGCACATCTGGGCGATAACGGTCGGAAAGGTTCTATTGGCATGCCATGTTATAATAAAGCCAGAGGCAAATGCAGATATGGTATTGGACAATGTTATAGAGTATATCAAAAGAGAGTACAACATCAGCCATGTCACCATTCAGATAGAGCGCCAGTAG

Protein sequence

MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLFIAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGDGVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIERQ
Homology
BLAST of Carg08521 vs. NCBI nr
Match: KAG6605616.1 (Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035528.1 Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 800.0 bits (2065), Expect = 9.5e-228
Identity = 421/421 (100.00%), Postives = 421/421 (100.00%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
           GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER
Sbjct: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420

Query: 421 Q 422
           Q
Sbjct: 421 Q 421

BLAST of Carg08521 vs. NCBI nr
Match: XP_022958195.1 (metal tolerance protein 1-like [Cucurbita moschata] >XP_022958198.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958199.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958200.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958201.1 metal tolerance protein 1-like [Cucurbita moschata])

HSP 1 Score: 783.5 bits (2022), Expect = 9.2e-223
Identity = 415/421 (98.57%), Postives = 416/421 (98.81%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQN DHGHMIEV GDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
           GVK  HHHHHHAHEESVTTPLLESHSQKVT TQKRERNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 241 GVK--HHHHHHAHEESVTTPLLESHSQKVTNTQKRERNINVQGAYLHVLGDSIQSIGVMI 300

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISH+TIQIER
Sbjct: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHITIQIER 419

Query: 421 Q 422
           Q
Sbjct: 421 Q 419

BLAST of Carg08521 vs. NCBI nr
Match: XP_023534426.1 (metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534427.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534428.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 782.3 bits (2019), Expect = 2.1e-222
Identity = 417/422 (98.82%), Postives = 417/422 (98.82%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHH-HPHHEEEGAAG 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHH HPHHEEEGAAG
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHPHPHHEEEGAAG 240

Query: 241 DGVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVM 300
           D VK   HHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVM
Sbjct: 241 DSVK---HHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVM 300

Query: 301 IGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLC 360
           IGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLC
Sbjct: 301 IGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLC 360

Query: 361 EMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIE 420
           EMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIE
Sbjct: 361 EMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIE 419

Query: 421 RQ 422
           RQ
Sbjct: 421 RQ 419

BLAST of Carg08521 vs. NCBI nr
Match: XP_022995643.1 (metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995644.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995646.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995647.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 773.5 bits (1996), Expect = 9.5e-220
Identity = 410/421 (97.39%), Postives = 413/421 (98.10%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQNHDHGHMIEV GDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGHSHGHGDH+HG G HEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
            VK   H HHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQS+GVM+
Sbjct: 241 SVK---HQHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGVMV 300

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER
Sbjct: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 418

Query: 421 Q 422
           Q
Sbjct: 421 Q 418

BLAST of Carg08521 vs. NCBI nr
Match: XP_022995642.1 (metal tolerance protein 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 773.5 bits (1996), Expect = 9.5e-220
Identity = 410/421 (97.39%), Postives = 413/421 (98.10%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQNHDHGHMIEV GDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 3   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 62

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 63  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 122

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 123 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 182

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGHSHGHGDH+HG G HEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD
Sbjct: 183 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 242

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
            VK   H HHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQS+GVM+
Sbjct: 243 SVK---HQHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGVMV 302

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 303 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 362

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER
Sbjct: 363 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420

Query: 421 Q 422
           Q
Sbjct: 423 Q 420

BLAST of Carg08521 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 527.3 bits (1357), Expect = 1.6e-148
Identity = 300/423 (70.92%), Postives = 337/423 (79.67%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           ME  +  H H++EV+         IV SK+CGEAP CGF D+KN+S D  ER+ASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V C+VFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGH+HGH+H HGH HG   HDH           H H HG++VT HHHHH H  E G    
Sbjct: 181 LGHDHGHSHGHGHGHG---HDH-----------HNHSHGVTVTTHHHHHDH--EHG---- 240

Query: 241 GVKHHHHH---HHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIG 300
              H H H    HHAH + VT  LL+    +V   +KR+RNIN+QGAYLHVLGDSIQS+G
Sbjct: 241 ---HSHGHGEDKHHAHGD-VTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVG 300

Query: 301 VMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKG 360
           VMIGGAIIWY PE+KI+DLICTL FSVIVL TTI M+RNILEVLMESTPREIDATKLEKG
Sbjct: 301 VMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKG 360

Query: 361 LCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQ 420
           L EMEEVVAVHELHIWAITVGKVLLACHV I+PEA+ADMVL+ VI+YI+REYNISHVTIQ
Sbjct: 361 LLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQ 398

BLAST of Carg08521 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 515.4 bits (1326), Expect = 6.1e-145
Identity = 289/417 (69.30%), Postives = 328/417 (78.66%), Query Frame = 0

Query: 6   HDHGHMIEVSGDVQAVGPSIVG-SKICGEAPSCGFKDAKNSSIDMRERNASMRKLFIAAV 65
           H+H HM+++ G+V +   S+VG  K CGEAP CGF DAK SSI+ +ER ASMRKL IA +
Sbjct: 21  HEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRKLLIAVL 80

Query: 66  CCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRI 125
            C +F+ +EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QSYGFFRI
Sbjct: 81  LCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGFFRI 140

Query: 126 EILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALLLGHE 185
           EILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA+LLGH+
Sbjct: 141 EILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHD 200

Query: 186 HGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGDGVKH 245
           HGH H H H +GHG H H HG            HGI+ T HHH   H E +         
Sbjct: 201 HGHGHGHSHDNGHG-HSHDHG------------HGIAATEHHHDSGHDESQ--------- 260

Query: 246 HHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMIGGAI 305
                       ++  L+E         QK++RN+N+QGAYLHVLGDSIQS+GVMIGGAI
Sbjct: 261 ------------LSDVLIE---------QKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAI 320

Query: 306 IWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMEEV 365
           IWYKPE+KILDLICTL+FSVIVL TTI MLRNILEVLMESTPREID T LEKG+CE+EEV
Sbjct: 321 IWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEV 380

Query: 366 VAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIERQ 422
           VAVHELHIWAITVGK+LLACHV I+PEA ADMVLD +I+YIKRE+NISHVTIQIERQ
Sbjct: 381 VAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIERQ 393

BLAST of Carg08521 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 3.3e-138
Identity = 295/432 (68.29%), Postives = 328/432 (75.93%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           M+  N     + EV  D+ +      G+K+C  A +C F D+ NSS D RER ASMRKL 
Sbjct: 1   MDSHNSAPPQIAEVRMDISSSTSVAAGNKVCRGA-ACDFSDSSNSSKDARERMASMRKLI 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA + CI+FM++EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATP+QSYG
Sbjct: 61  IAVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLLAGILVYEAIVRLIN  GEV+G LMFAVSAFGL VNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGH+HGH H HGH HGH  HDH HG  +H   DH HH         H H HH E+G    
Sbjct: 181 LGHDHGHGHGHGHGHGH-SHDHDHGGSDH---DHHHHED-----QEHGHVHHHEDGHGNS 240

Query: 241 -GVKHHHH----HHHHAHEESVTTPLLESHS------QKVTKTQKRERNINVQGAYLHVL 300
             V  HHH    HHHH  EE    PLL+S +            +K  RNINV  AYLHVL
Sbjct: 241 ITVNLHHHPGTGHHHHDAEE----PLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVL 300

Query: 301 GDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREI 360
           GDSIQSIGVMIGGAIIWYKPE+KI+DLICTLIFSVIVL+TTI+MLRNILEVLMESTPREI
Sbjct: 301 GDSIQSIGVMIGGAIIWYKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREI 360

Query: 361 DATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREY 420
           DAT LE GL +M+ VVAVHELHIWAITVGKVLLACHV I  +A+AD +LD VI YIK EY
Sbjct: 361 DATSLENGLRDMDGVVAVHELHIWAITVGKVLLACHVTITQDADADQMLDKVIGYIKSEY 418

Query: 421 NISHVTIQIERQ 422
           NISHVTIQIER+
Sbjct: 421 NISHVTIQIERE 418

BLAST of Carg08521 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 9.9e-111
Identity = 233/394 (59.14%), Postives = 270/394 (68.53%), Query Frame = 0

Query: 28  SKICGEAPSCGFKDAKNSSIDMRERNASMRKLFIAAVCCIVFMSIEVVGGIKANSLAILT 87
           SK+CGE  +CGF     SS D ++R ASMRKL    V C++FMSIEVV GIKANSLAIL 
Sbjct: 6   SKVCGET-ACGF---STSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 88  DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 147
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 148 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGS 207
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHSH HG        
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSHDHG-------- 185

Query: 208 HEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGDGVKHHHHHHHHAHEESVTTPLLESHS 267
           H +G                                                       +
Sbjct: 186 HSYG-----------------------------------------------------ERA 245

Query: 268 QKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIV 327
           +++ +  K  RNINVQGAYLHVLGD IQSIGVMIGG +IWY P++K++DLICTL FSVIV
Sbjct: 246 EQLLEKSKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIV 305

Query: 328 LYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHV 387
           L TTI+MLR+ILEVLMESTPREIDA +LEKGL E+EEVV VHELHIWAITVGK L +CHV
Sbjct: 306 LGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHV 334

Query: 388 IIKPEANADMVLDNVIEYIKREYNISHVTIQIER 421
            ++PEA  +MVL+ VI+YI REY ISHVTIQIER
Sbjct: 366 KVRPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334

BLAST of Carg08521 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 6.5e-78
Identity = 165/390 (42.31%), Postives = 236/390 (60.51%), Query Frame = 0

Query: 36  SCGFKDAKNSSIDMRERNASMRKLFIAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSD 95
           SC F   ++   + +ER  S R+L       ++ MS+++VGG KANSLA++TDAAHLLSD
Sbjct: 35  SCAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSD 94

Query: 96  VAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGP 155
           VA   +SL ++  S WEA PR S+GF R+E+L A +S+Q+IWL++G++++EAI RL++  
Sbjct: 95  VAGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRS 154

Query: 156 GEVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHR 215
            EV G +MF +SAFG  +N+ M L LGH H H H          HDH             
Sbjct: 155 REVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHH----------HDH------------- 214

Query: 216 HHHGISVTAHHHHHPHHEEEGAAGDGVKHHHHHHHH----AHEESVTTPLLESHSQKVTK 275
                    HHHHH H           KH H HHH       EE    PL      K  K
Sbjct: 215 ---------HHHHHNH-----------KHQHQHHHKEVVAEEEEEEMNPL------KGEK 274

Query: 276 TQKRERNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTI 335
           +  +E NIN+QGAYLH + D IQS+GVMIGG IIW KP++ ++DLICTL+FS   L  T+
Sbjct: 275 SSSKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATL 334

Query: 336 QMLRNILEVLMESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPE 395
            +L+NI  +LME  PR++D  KLE+GL  ++ V  V++LH+W ITVG+++L+CH++ +P 
Sbjct: 335 PILKNIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPG 375

Query: 396 ANADMVLDNVIEYIKREYNISHVTIQIERQ 422
           A+   ++  V  + ++ Y I H T+Q+E +
Sbjct: 395 ASPKEIITGVRNFCRKSYGIYHATVQVESE 375

BLAST of Carg08521 vs. ExPASy TrEMBL
Match: A0A6J1H2S0 (metal tolerance protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459497 PE=4 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.5e-223
Identity = 415/421 (98.57%), Postives = 416/421 (98.81%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQN DHGHMIEV GDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
           GVK  HHHHHHAHEESVTTPLLESHSQKVT TQKRERNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 241 GVK--HHHHHHAHEESVTTPLLESHSQKVTNTQKRERNINVQGAYLHVLGDSIQSIGVMI 300

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISH+TIQIER
Sbjct: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHITIQIER 419

Query: 421 Q 422
           Q
Sbjct: 421 Q 419

BLAST of Carg08521 vs. ExPASy TrEMBL
Match: A0A6J1JZI6 (metal tolerance protein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.6e-220
Identity = 410/421 (97.39%), Postives = 413/421 (98.10%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQNHDHGHMIEV GDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGHSHGHGDH+HG G HEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
            VK   H HHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQS+GVM+
Sbjct: 241 SVK---HQHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGVMV 300

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER
Sbjct: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 418

Query: 421 Q 422
           Q
Sbjct: 421 Q 418

BLAST of Carg08521 vs. ExPASy TrEMBL
Match: A0A6J1JZI0 (metal tolerance protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.6e-220
Identity = 410/421 (97.39%), Postives = 413/421 (98.10%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQNHDHGHMIEV GDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF
Sbjct: 3   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 62

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 63  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 122

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 123 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 182

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGHSHGHGDH+HG G HEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD
Sbjct: 183 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 242

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
            VK   H HHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQS+GVM+
Sbjct: 243 SVK---HQHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGVMV 302

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 303 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 362

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER
Sbjct: 363 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420

Query: 421 Q 422
           Q
Sbjct: 423 Q 420

BLAST of Carg08521 vs. ExPASy TrEMBL
Match: A7L8D2 (Metal tolerance protein OS=Cucumis sativus OX=3659 GN=MTP PE=2 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.2e-198
Identity = 376/421 (89.31%), Postives = 393/421 (93.35%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQ  DHGH+IEV GDVQAVGPSIVGSKICG+APSCGF DAKNSS D +ER+ASMRKL 
Sbjct: 1   MEVQ--DHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKLC 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKG LMFAVS FGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGFLMFAVSTFGLVVNIAMALL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGH+HGHG+HDHGHGSHEHG EDHRH HGISVT HHH   HHEE+ AA D
Sbjct: 181 LGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHH---HHEEKRAASD 240

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
           GV+HHHHHHHH H+ES T PLL+S SQKVTK QK++RNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 241 GVEHHHHHHHHKHKESTTVPLLDS-SQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMI 300

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEY ILDLICTLIFS IVL TTIQMLRNILEVLMESTPRE+DATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDATKLEKGLCE 360

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVA+HELHIWAITVGK+LLACHVIIKPEANADMVLD VIEYI+REYNISHVTIQIER
Sbjct: 361 MEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIER 415

Query: 421 Q 422
           Q
Sbjct: 421 Q 415

BLAST of Carg08521 vs. ExPASy TrEMBL
Match: A0A0A0KD36 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G080940 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.2e-198
Identity = 376/421 (89.31%), Postives = 393/421 (93.35%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           MEVQ  DHGH+IEV GDVQAVGPSIVGSKICG+APSCGF DAKNSS D +ER+ASMRKL 
Sbjct: 8   MEVQ--DHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKLC 67

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 68  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 127

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKG LMFAVS FGLVVNIAMALL
Sbjct: 128 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGFLMFAVSTFGLVVNIAMALL 187

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGHEHGHAHSHGH+HGHG+HDHGHGSHEHG EDHRH HGISVT HHH   HHEE+ AA D
Sbjct: 188 LGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHH---HHEEKRAASD 247

Query: 241 GVKHHHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMI 300
           GV+HHHHHHHH H+ES T PLL+S SQKVTK QK++RNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 248 GVEHHHHHHHHKHKESTTVPLLDS-SQKVTKAQKKQRNINVQGAYLHVLGDSIQSIGVMI 307

Query: 301 GGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360
           GGAIIWYKPEY ILDLICTLIFS IVL TTIQMLRNILEVLMESTPRE+DATKLEKGLCE
Sbjct: 308 GGAIIWYKPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDATKLEKGLCE 367

Query: 361 MEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIER 420
           MEEVVA+HELHIWAITVGK+LLACHVIIKPEANADMVLD VIEYI+REYNISHVTIQIER
Sbjct: 368 MEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQIER 422

Query: 421 Q 422
           Q
Sbjct: 428 Q 422

BLAST of Carg08521 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 527.3 bits (1357), Expect = 1.1e-149
Identity = 300/423 (70.92%), Postives = 337/423 (79.67%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           ME  +  H H++EV+         IV SK+CGEAP CGF D+KN+S D  ER+ASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V C+VFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGH+HGH+H HGH HG   HDH           H H HG++VT HHHHH H  E G    
Sbjct: 181 LGHDHGHSHGHGHGHG---HDH-----------HNHSHGVTVTTHHHHHDH--EHG---- 240

Query: 241 GVKHHHHH---HHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIG 300
              H H H    HHAH + VT  LL+    +V   +KR+RNIN+QGAYLHVLGDSIQS+G
Sbjct: 241 ---HSHGHGEDKHHAHGD-VTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVG 300

Query: 301 VMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKG 360
           VMIGGAIIWY PE+KI+DLICTL FSVIVL TTI M+RNILEVLMESTPREIDATKLEKG
Sbjct: 301 VMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKG 360

Query: 361 LCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQ 420
           L EMEEVVAVHELHIWAITVGKVLLACHV I+PEA+ADMVL+ VI+YI+REYNISHVTIQ
Sbjct: 361 LLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQ 398

BLAST of Carg08521 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 527.3 bits (1357), Expect = 1.1e-149
Identity = 300/423 (70.92%), Postives = 337/423 (79.67%), Query Frame = 0

Query: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60
           ME  +  H H++EV+         IV SK+CGEAP CGF D+KN+S D  ER+ASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120
           IA V C+VFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240
           LGH+HGH+H HGH HG   HDH           H H HG++VT HHHHH H  E G    
Sbjct: 181 LGHDHGHSHGHGHGHG---HDH-----------HNHSHGVTVTTHHHHHDH--EHG---- 240

Query: 241 GVKHHHHH---HHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIG 300
              H H H    HHAH + VT  LL+    +V   +KR+RNIN+QGAYLHVLGDSIQS+G
Sbjct: 241 ---HSHGHGEDKHHAHGD-VTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVG 300

Query: 301 VMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKG 360
           VMIGGAIIWY PE+KI+DLICTL FSVIVL TTI M+RNILEVLMESTPREIDATKLEKG
Sbjct: 301 VMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKG 360

Query: 361 LCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQ 420
           L EMEEVVAVHELHIWAITVGKVLLACHV I+PEA+ADMVL+ VI+YI+REYNISHVTIQ
Sbjct: 361 LLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQ 398

BLAST of Carg08521 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 515.4 bits (1326), Expect = 4.4e-146
Identity = 289/417 (69.30%), Postives = 328/417 (78.66%), Query Frame = 0

Query: 6   HDHGHMIEVSGDVQAVGPSIVG-SKICGEAPSCGFKDAKNSSIDMRERNASMRKLFIAAV 65
           H+H HM+++ G+V +   S+VG  K CGEAP CGF DAK SSI+ +ER ASMRKL IA +
Sbjct: 60  HEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRKLLIAVL 119

Query: 66  CCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRI 125
            C +F+ +EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QSYGFFRI
Sbjct: 120 LCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGFFRI 179

Query: 126 EILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALLLGHE 185
           EILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA+LLGH+
Sbjct: 180 EILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHD 239

Query: 186 HGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGDGVKH 245
           HGH H H H +GHG H H HG            HGI+ T HHH   H E +         
Sbjct: 240 HGHGHGHSHDNGHG-HSHDHG------------HGIAATEHHHDSGHDESQ--------- 299

Query: 246 HHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMIGGAI 305
                       ++  L+E         QK++RN+N+QGAYLHVLGDSIQS+GVMIGGAI
Sbjct: 300 ------------LSDVLIE---------QKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAI 359

Query: 306 IWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMEEV 365
           IWYKPE+KILDLICTL+FSVIVL TTI MLRNILEVLMESTPREID T LEKG+CE+EEV
Sbjct: 360 IWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEV 419

Query: 366 VAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIERQ 422
           VAVHELHIWAITVGK+LLACHV I+PEA ADMVLD +I+YIKRE+NISHVTIQIERQ
Sbjct: 420 VAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIERQ 432

BLAST of Carg08521 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 515.4 bits (1326), Expect = 4.4e-146
Identity = 289/417 (69.30%), Postives = 328/417 (78.66%), Query Frame = 0

Query: 6   HDHGHMIEVSGDVQAVGPSIVG-SKICGEAPSCGFKDAKNSSIDMRERNASMRKLFIAAV 65
           H+H HM+++ G+V +   S+VG  K CGEAP CGF DAK SSI+ +ER ASMRKL IA +
Sbjct: 21  HEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRKLLIAVL 80

Query: 66  CCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRI 125
            C +F+ +EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QSYGFFRI
Sbjct: 81  LCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGFFRI 140

Query: 126 EILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALLLGHE 185
           EILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA+LLGH+
Sbjct: 141 EILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHD 200

Query: 186 HGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGDGVKH 245
           HGH H H H +GHG H H HG            HGI+ T HHH   H E +         
Sbjct: 201 HGHGHGHSHDNGHG-HSHDHG------------HGIAATEHHHDSGHDESQ--------- 260

Query: 246 HHHHHHHAHEESVTTPLLESHSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMIGGAI 305
                       ++  L+E         QK++RN+N+QGAYLHVLGDSIQS+GVMIGGAI
Sbjct: 261 ------------LSDVLIE---------QKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAI 320

Query: 306 IWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMEEV 365
           IWYKPE+KILDLICTL+FSVIVL TTI MLRNILEVLMESTPREID T LEKG+CE+EEV
Sbjct: 321 IWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEV 380

Query: 366 VAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIERQ 422
           VAVHELHIWAITVGK+LLACHV I+PEA ADMVLD +I+YIKRE+NISHVTIQIERQ
Sbjct: 381 VAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIERQ 393

BLAST of Carg08521 vs. TAIR 10
Match: AT3G61940.1 (Cation efflux family protein )

HSP 1 Score: 401.7 bits (1031), Expect = 7.0e-112
Identity = 233/394 (59.14%), Postives = 270/394 (68.53%), Query Frame = 0

Query: 28  SKICGEAPSCGFKDAKNSSIDMRERNASMRKLFIAAVCCIVFMSIEVVGGIKANSLAILT 87
           SK+CGE  +CGF     SS D ++R ASMRKL    V C++FMSIEVV GIKANSLAIL 
Sbjct: 6   SKVCGET-ACGF---STSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 88  DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 147
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 148 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGHAHSHGHSHGHGDHDHGHGS 207
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHSH HG        
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSHDHG-------- 185

Query: 208 HEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGDGVKHHHHHHHHAHEESVTTPLLESHS 267
           H +G                                                       +
Sbjct: 186 HSYG-----------------------------------------------------ERA 245

Query: 268 QKVTKTQKRERNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSVIV 327
           +++ +  K  RNINVQGAYLHVLGD IQSIGVMIGG +IWY P++K++DLICTL FSVIV
Sbjct: 246 EQLLEKSKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIV 305

Query: 328 LYTTIQMLRNILEVLMESTPREIDATKLEKGLCEMEEVVAVHELHIWAITVGKVLLACHV 387
           L TTI+MLR+ILEVLMESTPREIDA +LEKGL E+EEVV VHELHIWAITVGK L +CHV
Sbjct: 306 LGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHV 334

Query: 388 IIKPEANADMVLDNVIEYIKREYNISHVTIQIER 421
            ++PEA  +MVL+ VI+YI REY ISHVTIQIER
Sbjct: 366 KVRPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6605616.19.5e-228100.00Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_022958195.19.2e-22398.57metal tolerance protein 1-like [Cucurbita moschata] >XP_022958198.1 metal tolera... [more]
XP_023534426.12.1e-22298.82metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534427.1 meta... [more]
XP_022995643.19.5e-22097.39metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995644.1 met... [more]
XP_022995642.19.5e-22097.39metal tolerance protein 1-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZT631.6e-14870.92Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2[more]
Q9LXS16.1e-14569.30Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2[more]
Q688R13.3e-13868.29Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 ... [more]
Q9M2719.9e-11159.14Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1[more]
Q6DBM86.5e-7842.31Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H2S04.5e-22398.57metal tolerance protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459497 PE=... [more]
A0A6J1JZI64.6e-22097.39metal tolerance protein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1JZI04.6e-22097.39metal tolerance protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A7L8D22.2e-19889.31Metal tolerance protein OS=Cucumis sativus OX=3659 GN=MTP PE=2 SV=1[more]
A0A0A0KD362.2e-19889.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G080940 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G46800.11.1e-14970.92zinc transporter of Arabidopsis thaliana [more]
AT2G46800.21.1e-14970.92zinc transporter of Arabidopsis thaliana [more]
AT3G58810.14.4e-14669.30metal tolerance protein A2 [more]
AT3G58810.24.4e-14669.30metal tolerance protein A2 [more]
AT3G61940.17.0e-11259.14Cation efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 56..421
e-value: 1.2E-97
score: 324.6
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 59..342
e-value: 3.4E-47
score: 160.8
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 55..341
e-value: 5.9E-49
score: 168.4
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 53..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..242
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..274
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 194..219
NoneNo IPR availablePANTHERPTHR11562:SF88METAL TOLERANCE PROTEIN A1coord: 1..420
NoneNo IPR availablePANTHERPTHR11562CATION EFFLUX PROTEIN/ ZINC TRANSPORTERcoord: 1..420
IPR036837Cation efflux protein, cytoplasmic domain superfamilySUPERFAMILY160240Cation efflux protein cytoplasmic domain-likecoord: 348..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08521-RACarg08521-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071577 zinc ion transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0005385 zinc ion transmembrane transporter activity
molecular_function GO:0008324 cation transmembrane transporter activity