Carg08500 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08500
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionE3 ubiquitin ligase
LocationCarg_Chr02: 5353134 .. 5360790 (+)
RNA-Seq ExpressionCarg08500
SyntenyCarg08500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTGTTTTTTTAGTTAATCATTAGTCTTTCAAACGCCGGTGTTTTGGTTCTGAGGCGCTGCAAACTCATCTCTGGGAAGAAATCTGGCTGTGGAGGGTACTCTTTTATATTCTCTACCTCTGTTATCTTTACCCTGTTTTTCCCTTCTTATATTGGGACTTTGCTGCGATCACAGCCATGAAATTGGTTGTTCCCTTGGAGGATCTTCCCAACAAATTGGTCTCTGATTTGTCCATTATTGACATGGTTTCACCATTGATCTGATACCACAGAAGCCACAGAGTAGCATGATTATGGAGGGGATGGAAGAGGAAGCCGATAAAGATCTTATGTTGCTGCCTTACTCTTCTCCAGCTGTAGCAGTTGCTATCAGTGGGAACAAAAGCAGTAAACATATAATTAGGTGGTCCTTGGAAAATTTTCGACACAAGGGCATCATTAGTTTCAAGTTGATACACATCATCCCAAGGATTACTAGTGTGCCAACTCCAAGTAAGCCATATACATTCTGTTCATGTTTCTGATGTGATTTCTTTTAATGATGCTATTATCTAACAACCGTGTAACTTTACATTAGTATGATATTGTCCGGTTTGAACATAAGCTCTTGTGGCTTTATTTTTTGTTTCTCCAAAAGGTCTCATACCAATAGTGACAATGTTTCTCACTTAGATACCCGTAATCCTCCGTGGATTTAACAATCCTCTCATCGAACAAAGTACACTAAGAGCCTCCCCTGATGCCTCTCTCCCTAGAGCCCTCAAACAAAATATACCCTTTGCTTGATACCTGAGACACCATGACTACATATTCGAGGCTCGTAACTTCTTTGTTTGACATTGAAGGTTGAACATAACTTTGATACCAGATGGTTGAACATAACTCATTTGTGGGAAACACTCACACCCTTTATTAAGACCAATCAAGAAGATATTAAAAGATGCTCTATAAGAACTACTACGCTTGCTTTGCTTGGAATTGCTCGAGATGAAAATTTTAATGGGATAGAAGGATATTTATAGTAAAGGCGGTTGAGGATTGTTGGGAGGGAGTCCCACGTTGCCTAATTAGGGGATGATCATAGGTTTATAAGTAAGAAACACATCTCCATTAGTATGAGACCTTTTAAGAAAATCAAAAGCAAAGTCATGAGAGCTAATGCTCAAAGTGGACAATATCAGACCATTGTCGAAGTTCGTGATTCCTAACAAAGGTTAGATAGTGTCGAGTAGATTTATTCTTAAAATATATAGACAAGGGGCTCATACAATTATTGGACTCGGTTATCAAATTTTCCTTTGCTTCTTGGCTAATGCTATACTTGTCTAGATTGCGTATTCAAGCTTTATGCCAATGTTGATGCAGTGGGAAACTCAATTCCAGTTTCACAAGTTCGTGAAGATGTTGCTGCGGCTTATAGGAAAGAAATGGGGTGGCAGACAAGTGAAAAGCTTGTTCCATATAAGAAGATGTTTGCTCAGAGAAAGGTCCAGCCTTACTCTAATTTGAATTATGGGTAGCAAAAAAGAATGAACTTGAGTCTTCTTTCGAATTGTAGGTACAGCATGATGTTGTGACTCTTGAAGCAGATGATGTTGCAAATGCAATAATTGAAGAGGTTACAAGGTGTTCAATAAGCAAGCTCGTTATAGGGGTTTCATCACACGGATTTTTCTCCAGGTATTTAACTACCTTTTAGCCTGTTCTTGCTGAATTCTTTATAAGGGTTGGAAACCTCATTTTAAAAACCTTGAGGGGAAGTCCGAAATGGAAATCCTAAAAATGACAATATCTGTTAGCGGTGGACTTGGCCCGTTACAAATAGTATCAGAGCTAGACACCGGGTGATGTGCCAACGAGGAAGCTAAGCCTCGAAGATGGGTGGACACGAGGCGGTGTGCCAACAAGGACGCTGGCCCCAAAGCGGTGGATTGTGGGGGTCCCACATCAATTGGAGAAAGGAACTAGTGCCAGCGAGGACGTTGGGCCCAGAAGGAGGGTGGATTGTGAGATCCCACATCGGTTGGGAGGAGAACCATTCTTTATAAGGGTTGGAAACCTCTCCCTAGCAGACGCGTTTTAAAAACATTGAGTGAAAACTCGAAAAGGAAACCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGACCCTTACAAATGGTATCAGAGCTAAACACTGGGTGATGTGCCAGTGAGGAGACTGAACCCTAAAAGGGGTGGACACGAGGCGGTGTGCCAGCAAGGACGCTGGACCCTGAAGGGAGTGGACTGAGGGGTCCCACATCGATTGGAGAAGGGAACGAATGCCAACGAGAACGTTGGACCTTGAAGGGGGGTGGATTATGAGATCTTACATCAGTTGGGAGGAGAACGAAACATTTTTTTATAAGGGTGTGAAAACTTATCTCTAGCAGACACATTTTAAAAACCTTGAGGGGAAGCCCGAAAGGGAAAGTCCAAGAAAGACAATATGTGCTAGCGGTGGAGTTGGACGGCGTTTATTTTCTCTTTCTATGTTGTTAACCCACTTGTGTCTGGATGACATTCTTTTATAGGAAACTAAACGGTCTGTCCTCAAGAATATCAGCTCATGCACCTAGATATTGTACGGTCTATGCCATTTCAAAAGGAAAATTAGCCTCAATACGGCCACCTGATATGGAGACAAGCGTGAGCATTAAAGATGATTCGAGTGAAGCAAGTTCTGCACATAGCTACTCAAGCTATTCTTCTAGCTCACTTACAGGTAGCCATTAATTAGTTAAGCATTCAACTAGAGACATGGATCGTTGTCTTTACGTTATATACCTAGCTACATTTCTCGGACCTGGTTAAATTTTGTGATTTCCGTAATGACCCGACTTTTTGAACTTTACCTGACTAAGGTCATTACTACATGCATACTAAAAGCAGTTGAAAACTTGCGCGGAATCTTTATTGACTTTGAACATTCAACAAACAACCCCATATCACTTGCCTTGTACGTCCTGCCTTTACCTAGTATGTAGCTCCACTGTTGAGGTCGAGATATGCAATCACATGCAATCATGATTTAGGTTAACATGATTATAATGGAACCTAAAACTTAGTGTTCTGGCTTCTACTTTTTTTTCTTTTCTTCGGGTTCCATATATATCATTTTCATTTACATCAAACCTTCTAGGCCTCTCCAGATATGCTCTTTTATGCATAGATGTGCATTACTTTCATACACTTCGTTCTGTTTTCTTGCTGCAGATAGCAGTTCGAGCTTAATTACATCCTACTCTCATTTCCCTTCTCGTTCCCCTTCTCGACCATTACAACGGTTTCAAGCTCTTTCGACCATCAATCAGTCTCTTCGTACTACAAAAAGTAGCCCCATTGTAGCCAACCATTCTCGATGCCATTCCGTTGATATTGAGGAGCCGGTGGATCGCCTTCGATCCTCTCCCAATGTTTCATATTGTGCACAAACATTGAGTCGAGCCTCTAGTTGTAAAAGCTTGCCAACAGATTACAAGTCATGGGTTCCTGATGAAGCTTCATCATCAGGCACATTCAATGATTATGTTTCATGTGAAAGTCAGGTAATCTATCGAAGGTGTCGTTTGGAGGATAATGTTCGAGTTCAGTACGGTCGGATCAATTATTGAGATTGATAGTTTGACGACCCACGACTTTGATGTTCGCATTATGACATTCATTTATTTGATGTTAGAATGATGTGTTTGTATGTTTAAGTTGTTCACTTGCATTGGCAGGCGGATGTCAACTTTGAGCTTGAAAAGTTGAGAATTGAACTAAAACATGCAAGAGAAATGTACGCAATTGTTCAGCAAGAGACGGCCAATGCCACTTTGAAGGTAGATGTTATGTTTGTTATGATTTATAGTTAATGGTACTCTCTTTAGTGGTTTCCTTCTCATGGTTAGAGAGAGGGTTGTTCTTAGCAACCATCATCATCGACACCGTTGGTTGGAGAGGGGAACGAAACACCCTTTATAAGGGTGTGGAAGCCTCTCCCTAACAAAAGCGTTTAAAAACCGTGAGGTTGATAGCAATACGTAATAGGCCACACAATATCTGTTGGCAGTGGACAAATACGTAACAGGCCAAAGCGGACAATATTTGCTAGCAATGGGCTTGGATAGTTACAAATGGTATTAGAGCCAGACACTGGATGGTCTGCCAGCGAGGACGTTGGGTTGTGAGATCCCACATTGGTTGGAGAGAGAAACAAAGCACCCTTTATTTGTGTGGAAACCTCTCCGTAACAAACGTATTTTAAAATCGTGAGGCTGACGGTTGTTAGCTATGGGCTTGGGTGGTTACAAATGGTACCAGAGCCAGACACCGAGTGGTATGTCAGCAAGAATTCTGACTCTCAAGGAGGGTGGATTGTGAGATCCTACATTGGTTGGAGAGGGGAACAAAACACCCTTAAGGTGTGGAAACCTCTCCCTAACAAACGTGTTTTAAAGCGGTGAGGCTGATGACGATACGTAACGGACCAAAGCGGACAATATCTACTAGCAGTGGGTTTGGGCTGTTACACTACCGATTGTATACATGTTAGGCTTCTTATTTGCTTATGACATATAGCAAATTCAGGGTGTTATTGCTTTTGATCAGGGGAGCCATCTAAATAAACAACGATCGAAAGAAGCTAAGAAGCTTGAGGAGATCAACAATAAAGATGTATCTGGTAAAGAATTTGCAAGGGAAAAGAGAGCAAATGTTGAAGCTTTGAGAAGAGAAGCAAACTTTGTAAGAGAAGGTGCTGAAAGAGAGGGTATCTATAGGAAGGAAGCAGAGACGAAAGCTGTTGAAGATGCCAAAGAGAAGGGAAAGCATGAGAGTGCTCTTCATGGATATCTGCAGCAATACCAACATTTTGAATGGGATGATATTGTATCTGCTGCATCATCTTTCTCTGAGGATCTTATGATTGGAATGGGAGCATATGGAGCAGTTTATAAGTGTAATTTGCATCATACAACCGTAGCAGTGAAGGTTCTTCACTCTAGAGATGATCACAAGAACACCCAATTTCTTCGGGAGGTGAGTGAATTATTCTAAATCGGATTTGGTTAGGTTCTTGATAAAAAAAATGAGGCCTTCTAGTTATTGTGAGATCCCATATCGGTTCGAGAAGAGAATGAAACATTCCGAGTGTGGAAACCTCTCCCTAGTAGACGTGTTTTAAAATCGTGGGGATGATGATGATGTGTAACGAGCCAAAGCGGACAATGTCTGCTAGCGGTGGGCTTATACTTTCACAAATGGTATTAGAGTCAGTCACCAGATGGTGCGAGACATTGGTCGGAGTAGGGCTAGACCTTCTCCATAGTAGACGCGTTTCAAAACTGTGAGGTTGACGGCGATACGTAATAAGCCAAAGCGGACAATATTTGTTAACAATGAGATTGAGTTGTTACAAATGGTATCAGAAGCAGACACTGGCCGGTGTGCCAGCGAGGACGCTGGGCCCCCAAGGGGGTGGATTGTGAGATCCTCATATCGGTTGGAGAGGGGAATGAAGCATTTCTTATAAAGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAGAACTGTGAGGTTGTCAGTGATATATAACAGGCCAAAGCGGATAATATCTGCTAGCAGTGGGCTTGGGCTGTTACAAATGGTATCAAAGTCGGTCACTGGACGGTGCAAGGCATTGGTCGGAGTAGGGCTAGACTCTCTCCATTGTAGACGCGTTTCATAACTGTGAGACTGACGATAATACGTAATAGGCCAAAGCGGACAATATCTATTAACAGTGAGTTTGGGTTGTTACAAATGATTTCAGAGTCCAGACACTGGATAGTGTGCCAGCGAGGAGACGCTGGACCCCCAAGGGAGGTGGATTGTGAGATCTCATATCAATTGGAGAGGGGAACGAACCATTCCTTATAAGGGTGTGGAAACCTCTCCTTAGTAGACATGTTCGTGAGGCTAATAGCGATACGCTGAAACTTCGCTTGATTTTTTGTTCTGCTCTATATTGTATGATTATCCTTACTGAACTGATATTTATATCTTCTGTTGCCTTTCAGCTTGAAATCTTGAGCAAAATTCATCATCCCCATTTACTACTACTCCTTGGTGCGTGTCCGGACAAGCACTGCCTGGTTTATGAGTACATGGAAAACGGTAGCCTAGAGGATAGGTTGTACCGCAGAGGCAACACACCTGCAATCCCATGGTACGAGAGGTATAGGATTGCTTGGGAAATAGCCTCAGCTCTTGTCTTTCTACACAGCTCAAAGCCGAAACCGATAATCCACCGTGATCTTAAGCCTGCAAACATCTTACTCGACCGAAACCTCGTGAGCAAAATCGGCGACGTCGGTCTATCTACTATGCTTAATTCAGATCCTTTAATGTCTACTGCATTCAAGGATAGTGGACTAGTTGGAACTCTATGTTATATAGATCCTGAGTATCAACGAACTGGATTGATCTCACCTAAGTCTGATGTTTATGCTTTTGGAATGGTGATCTTGCAGTTACTAACTTCAAAGCCAGTAGTAGCATTAACCCATGTGGTGGAAACAGCCATTGAGAAGCGCTCTTTAATAAAGGTTCTTGATATTGAGGCTGGGCATTGGCCTACCGAAGAGACGTATGAATTGGCGAGATTGGGACTTTGGTGTGCAGAGCTACAACGTAAAGATAGGCCCAACTTAAAGGATCAAGTACTTCCCTTGTTAGTGAGACTCAGAAAGGTTGCTGATGAGGCTCGCAATTCGGCCTCCAGAGTCCCACCTACCATTCCTAATCATTTCATCTGTCCAATTCTTATGGTTAGTTGAATCATCCTGTGTCTTACTATTATTCGCTTCGACGATTGGATGATCTTTAAATTTAGACTTGTTCTTCCTCGAAGAATTGGATATAGACACTTCCAATAAGATTTCGTTGGGGAATAAAAATTCATCGAAGTCAAGAGACTCTATCCTAGTCTGATTGGCAATCTTCTTGGAAGTTTAACAGTTTGTTTCTATATGGCCCCCATGTGTCGGGAGTCCAAGCCCACCGCTAATAAATAATGTCCTATTTAGGCTCTCTTTTGAGTTTTGGCTTAAGGTTTTAAAACGAGTCTGCTAGGAAGAGGTTTTCACATCTTTATAAGGGATATTTTGTTCTTTTGAGTTTTGGCTTAAGGTTTCAATGTACTTGTGCAAGTGCATATCAGATCATAAGTTACTTTTTTGCTAGTTAGTTTCCAACCATATATTTTTCTTGAACATACTAGATTATTTTCCAACAGGTTGTGATGAATGACCCTTGTGTTGCGGCGGATGGATACACATACGATCGTCGAGCGATAGAGAAATGGTTCCAAGAGAACGATATTTCACCAACGACGAAGTTGCCACTGCCAGATAAGAATTTAATACCTAATCACGGTCTTCTGTCTTCAATTATGGAGTGGAACTCCAGAAAAAACTAATGCCATTTTGAAGGTTTGAACAGAGTTAGCATGTGAAGCTGGATGAGTAGCCACTATTTACCTTCAACTTCTTTCCCCATAGCCATTATAGGAAATCTTGACTTGCAGAGAAAAGAATATGATCCTGCACAAAACTTAATAATAATTCTAAACTATTATATATAAAGAGACAATAATCATTGATTTTCAC

mRNA sequence

CCTTTGTTTTTTTAGTTAATCATTAGTCTTTCAAACGCCGGTGTTTTGGTTCTGAGGCGCTGCAAACTCATCTCTGGGAAGAAATCTGGCTGTGGAGGAAGCCACAGAGTAGCATGATTATGGAGGGGATGGAAGAGGAAGCCGATAAAGATCTTATGTTGCTGCCTTACTCTTCTCCAGCTGTAGCAGTTGCTATCAGTGGGAACAAAAGCAGTAAACATATAATTAGGTGGTCCTTGGAAAATTTTCGACACAAGGGCATCATTATGGGAAACTCAATTCCAGTTTCACAAGTTCGTGAAGATGTTGCTGCGGCTTATAGGAAAGAAATGGGGTGGCAGACAAGTGAAAAGCTTGTTCCATATAAGAAGATGTTTGCTCAGAGAAAGGTACAGCATGATGTTGTGACTCTTGAAGCAGATGATGTTGCAAATGCAATAATTGAAGAGGTTACAAGATATTGTACGGTCTATGCCATTTCAAAAGGAAAATTAGCCTCAATACGGCCACCTGATATGGAGACAAGCGTGAGCATTAAAGATGATTCGAGTGAAGCAAGTTCTGCACATAGCTACTCAAGCTATTCTTCTAGCTCACTTACAGATAGCAGTTCGAGCTTAATTACATCCTACTCTCATTTCCCTTCTCGTTCCCCTTCTCGACCATTACAACGGTTTCAAGCTCTTTCGACCATCAATCAGTCTCTTCGTACTACAAAAAGTAGCCCCATTGTAGCCAACCATTCTCGATGCCATTCCGTTGATATTGAGGAGCCGGTGGATCGCCTTCGATCCTCTCCCAATGTTTCATATTGTGCACAAACATTGAGTCGAGCCTCTAGTTGTAAAAGCTTGCCAACAGATTACAAGTCATGGGTTCCTGATGAAGCTTCATCATCAGGCACATTCAATGATTATGTTTCATGTGAAAGTCAGGCGGATGTCAACTTTGAGCTTGAAAAGTTGAGAATTGAACTAAAACATGCAAGAGAAATGTACGCAATTGTTCAGCAAGAGACGGCCAATGCCACTTTGAAGAGAGCAAATGTTGAAGCTTTGAGAAGAGAAGCAAACTTTGTAAGAGAAGGTGCTGAAAGAGAGGGTATCTATAGGAAGGAAGCAGAGACGAAAGCTGTTGAAGATGCCAAAGAGAAGGGAAAGCATGAGAGTGCTCTTCATGGATATCTGCAGCAATACCAACATTTTGAATGGGATGATATTGTATCTGCTGCATCATCTTTCTCTGAGGATCTTATGATTGGAATGGGAGCATATGGAGCAGTTTATAAGTGTAATTTGCATCATACAACCGTAGCAGTGAAGCTTGAAATCTTGAGCAAAATTCATCATCCCCATTTACTACTACTCCTTGGTGCGTGTCCGGACAAGCACTGCCTGGTTTATGAGTACATGGAAAACGGTAGCCTAGAGGATAGGTTGTACCGCAGAGGCAACACACCTGCAATCCCATGGTACGAGAGGTATAGGATTGCTTGGGAAATAGCCTCAGCTCTTGTCTTTCTACACAGCTCAAAGCCGAAACCGATAATCCACCGTGATCTTAAGCCTGCAAACATCTTACTCGACCGAAACCTCGTGAGCAAAATCGGCGACGTCGGTCTATCTACTATGCTTAATTCAGATCCTTTAATGTCTACTGCATTCAAGGATAGTGGACTAGTTGGAACTCTATGTTATATAGATCCTGAGTATCAACGAACTGGATTGATCTCACCTAAGTCTGATGTTTATGCTTTTGGAATGGTGATCTTGCAGTTACTAACTTCAAAGCCAGTAGTAGCATTAACCCATGTGGTGGAAACAGCCATTGAGAAGCGCTCTTTAATAAAGGTTCTTGATATTGAGGCTGGGCATTGGCCTACCGAAGAGACGTATGAATTGGCGAGATTGGGACTTTGGTGTGCAGAGCTACAACGTAAAGATAGGCCCAACTTAAAGGATCAAGTACTTCCCTTGTTAGTGAGACTCAGAAAGGTTGCTGATGAGGCTCGCAATTCGGCCTCCAGAGTCCCACCTACCATTCCTAATCATTTCATCTGTCCAATTCTTATGGTTGTGATGAATGACCCTTGTGTTGCGGCGGATGGATACACATACGATCGTCGAGCGATAGAGAAATGGTTCCAAGAGAACGATATTTCACCAACGACGAAGTTGCCACTGCCAGATAAGAATTTAATACCTAATCACGGTCTTCTGTCTTCAATTATGGAGTGGAACTCCAGAAAAAACTAATGCCATTTTGAAGGTTTGAACAGAGTTAGCATGTGAAGCTGGATGAGTAGCCACTATTTACCTTCAACTTCTTTCCCCATAGCCATTATAGGAAATCTTGACTTGCAGAGAAAAGAATATGATCCTGCACAAAACTTAATAATAATTCTAAACTATTATATATAAAGAGACAATAATCATTGATTTTCAC

Coding sequence (CDS)

ATGATTATGGAGGGGATGGAAGAGGAAGCCGATAAAGATCTTATGTTGCTGCCTTACTCTTCTCCAGCTGTAGCAGTTGCTATCAGTGGGAACAAAAGCAGTAAACATATAATTAGGTGGTCCTTGGAAAATTTTCGACACAAGGGCATCATTATGGGAAACTCAATTCCAGTTTCACAAGTTCGTGAAGATGTTGCTGCGGCTTATAGGAAAGAAATGGGGTGGCAGACAAGTGAAAAGCTTGTTCCATATAAGAAGATGTTTGCTCAGAGAAAGGTACAGCATGATGTTGTGACTCTTGAAGCAGATGATGTTGCAAATGCAATAATTGAAGAGGTTACAAGATATTGTACGGTCTATGCCATTTCAAAAGGAAAATTAGCCTCAATACGGCCACCTGATATGGAGACAAGCGTGAGCATTAAAGATGATTCGAGTGAAGCAAGTTCTGCACATAGCTACTCAAGCTATTCTTCTAGCTCACTTACAGATAGCAGTTCGAGCTTAATTACATCCTACTCTCATTTCCCTTCTCGTTCCCCTTCTCGACCATTACAACGGTTTCAAGCTCTTTCGACCATCAATCAGTCTCTTCGTACTACAAAAAGTAGCCCCATTGTAGCCAACCATTCTCGATGCCATTCCGTTGATATTGAGGAGCCGGTGGATCGCCTTCGATCCTCTCCCAATGTTTCATATTGTGCACAAACATTGAGTCGAGCCTCTAGTTGTAAAAGCTTGCCAACAGATTACAAGTCATGGGTTCCTGATGAAGCTTCATCATCAGGCACATTCAATGATTATGTTTCATGTGAAAGTCAGGCGGATGTCAACTTTGAGCTTGAAAAGTTGAGAATTGAACTAAAACATGCAAGAGAAATGTACGCAATTGTTCAGCAAGAGACGGCCAATGCCACTTTGAAGAGAGCAAATGTTGAAGCTTTGAGAAGAGAAGCAAACTTTGTAAGAGAAGGTGCTGAAAGAGAGGGTATCTATAGGAAGGAAGCAGAGACGAAAGCTGTTGAAGATGCCAAAGAGAAGGGAAAGCATGAGAGTGCTCTTCATGGATATCTGCAGCAATACCAACATTTTGAATGGGATGATATTGTATCTGCTGCATCATCTTTCTCTGAGGATCTTATGATTGGAATGGGAGCATATGGAGCAGTTTATAAGTGTAATTTGCATCATACAACCGTAGCAGTGAAGCTTGAAATCTTGAGCAAAATTCATCATCCCCATTTACTACTACTCCTTGGTGCGTGTCCGGACAAGCACTGCCTGGTTTATGAGTACATGGAAAACGGTAGCCTAGAGGATAGGTTGTACCGCAGAGGCAACACACCTGCAATCCCATGGTACGAGAGGTATAGGATTGCTTGGGAAATAGCCTCAGCTCTTGTCTTTCTACACAGCTCAAAGCCGAAACCGATAATCCACCGTGATCTTAAGCCTGCAAACATCTTACTCGACCGAAACCTCGTGAGCAAAATCGGCGACGTCGGTCTATCTACTATGCTTAATTCAGATCCTTTAATGTCTACTGCATTCAAGGATAGTGGACTAGTTGGAACTCTATGTTATATAGATCCTGAGTATCAACGAACTGGATTGATCTCACCTAAGTCTGATGTTTATGCTTTTGGAATGGTGATCTTGCAGTTACTAACTTCAAAGCCAGTAGTAGCATTAACCCATGTGGTGGAAACAGCCATTGAGAAGCGCTCTTTAATAAAGGTTCTTGATATTGAGGCTGGGCATTGGCCTACCGAAGAGACGTATGAATTGGCGAGATTGGGACTTTGGTGTGCAGAGCTACAACGTAAAGATAGGCCCAACTTAAAGGATCAAGTACTTCCCTTGTTAGTGAGACTCAGAAAGGTTGCTGATGAGGCTCGCAATTCGGCCTCCAGAGTCCCACCTACCATTCCTAATCATTTCATCTGTCCAATTCTTATGGTTGTGATGAATGACCCTTGTGTTGCGGCGGATGGATACACATACGATCGTCGAGCGATAGAGAAATGGTTCCAAGAGAACGATATTTCACCAACGACGAAGTTGCCACTGCCAGATAAGAATTTAATACCTAATCACGGTCTTCTGTCTTCAATTATGGAGTGGAACTCCAGAAAAAACTAA

Protein sequence

MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGIIMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEVTRYCTVYAISKGKLASIRPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQALSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKRANVEALRREANFVREGAEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVKLEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLLVRLRKVADEARNSASRVPPTIPNHFICPILMVVMNDPCVAADGYTYDRRAIEKWFQENDISPTTKLPLPDKNLIPNHGLLSSIMEWNSRKN
Homology
BLAST of Carg08500 vs. NCBI nr
Match: KAG7035547.1 (U-box domain-containing protein 35 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1404.8 bits (3635), Expect = 0.0e+00
Identity = 713/713 (100.00%), Postives = 713/713 (100.00%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGIIMGNSIPVSQ 60
           MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGIIMGNSIPVSQ
Sbjct: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGIIMGNSIPVSQ 60

Query: 61  VREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEVTRYCTVY 120
           VREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEVTRYCTVY
Sbjct: 61  VREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEVTRYCTVY 120

Query: 121 AISKGKLASIRPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRS 180
           AISKGKLASIRPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRS
Sbjct: 121 AISKGKLASIRPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRS 180

Query: 181 PSRPLQRFQALSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSR 240
           PSRPLQRFQALSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSR
Sbjct: 181 PSRPLQRFQALSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSR 240

Query: 241 ASSCKSLPTDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQ 300
           ASSCKSLPTDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQ
Sbjct: 241 ASSCKSLPTDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQ 300

Query: 301 ETANATLKRANVEALRREANFVREGAEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQ 360
           ETANATLKRANVEALRREANFVREGAEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQ
Sbjct: 301 ETANATLKRANVEALRREANFVREGAEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQ 360

Query: 361 YQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVKLEILSKIHHPHLLLLLG 420
           YQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVKLEILSKIHHPHLLLLLG
Sbjct: 361 YQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVKLEILSKIHHPHLLLLLG 420

Query: 421 ACPDKHCLVYEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIH 480
           ACPDKHCLVYEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIH
Sbjct: 421 ACPDKHCLVYEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIH 480

Query: 481 RDLKPANILLDRNLVSKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLIS 540
           RDLKPANILLDRNLVSKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLIS
Sbjct: 481 RDLKPANILLDRNLVSKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLIS 540

Query: 541 PKSDVYAFGMVILQLLTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLG 600
           PKSDVYAFGMVILQLLTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLG
Sbjct: 541 PKSDVYAFGMVILQLLTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLG 600

Query: 601 LWCAELQRKDRPNLKDQVLPLLVRLRKVADEARNSASRVPPTIPNHFICPILMVVMNDPC 660
           LWCAELQRKDRPNLKDQVLPLLVRLRKVADEARNSASRVPPTIPNHFICPILMVVMNDPC
Sbjct: 601 LWCAELQRKDRPNLKDQVLPLLVRLRKVADEARNSASRVPPTIPNHFICPILMVVMNDPC 660

Query: 661 VAADGYTYDRRAIEKWFQENDISPTTKLPLPDKNLIPNHGLLSSIMEWNSRKN 714
           VAADGYTYDRRAIEKWFQENDISPTTKLPLPDKNLIPNHGLLSSIMEWNSRKN
Sbjct: 661 VAADGYTYDRRAIEKWFQENDISPTTKLPLPDKNLIPNHGLLSSIMEWNSRKN 713

BLAST of Carg08500 vs. NCBI nr
Match: KAG6605637.1 (U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1342.0 bits (3472), Expect = 0.0e+00
Identity = 709/811 (87.42%), Postives = 711/811 (87.67%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           MIMEGMEEEADKDLMLLP SSPAVAVAISGNKSSKHIIRWSLENFRHKGII         
Sbjct: 1   MIMEGMEEEADKDLMLLPSSSPAVAVAISGNKSSKHIIRWSLENFRHKGIISFKLIHIIP 60

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD
Sbjct: 61  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 121 DVANAIIEEVTRCSISKLVIGVSSHGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 180

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240
           RPPDMETSVSIKDDSSEASSAHSYS+YSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA
Sbjct: 181 RPPDMETSVSIKDDSSEASSAHSYSNYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240

Query: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300
           LSTINQSLRTTKSSPIVANHSRCHSVDIEEP+DRLRSSPNVSYCAQTLSRASSCKSLPTD
Sbjct: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPMDRLRSSPNVSYCAQTLSRASSCKSLPTD 300

Query: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL--- 360
           YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL   
Sbjct: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKVS 360

Query: 361 -----------------------------KRANVEALRREANFVREGAEREGIYRKEAET 420
                                        KRANVEALRREA FVREGAEREGIYRKEAET
Sbjct: 361 HLNKQRSKEAKKLEEMNNKEVSAKEFAREKRANVEALRREAKFVREGAEREGIYRKEAET 420

Query: 421 KAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHT 480
           KAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHT
Sbjct: 421 KAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHT 480

Query: 481 TVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL 540
           TVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL
Sbjct: 481 TVAVKVLHSRDDHKNIQFLRELEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL 540

Query: 541 YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG 600
           YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG
Sbjct: 541 YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG 600

Query: 601 LSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVV 660
           LSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVV
Sbjct: 601 LSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVV 660

Query: 661 ALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLL 714
           ALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLL
Sbjct: 661 ALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLL 720

BLAST of Carg08500 vs. NCBI nr
Match: XP_022957883.1 (U-box domain-containing protein 35-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 690/809 (85.29%), Postives = 698/809 (86.28%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           MIMEGMEEEADKDLMLLP SSPAVAVAISGNKSSKHIIRWSLENFRHKGII         
Sbjct: 1   MIMEGMEEEADKDLMLLPSSSPAVAVAISGNKSSKHIIRWSLENFRHKGIISFKLIHIIP 60

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD
Sbjct: 61  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 121 DVANAIIEEVTRCSISKLVIGVSSHGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 180

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF S SPSRPLQRFQA
Sbjct: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFHSPSPSRPLQRFQA 240

Query: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300
           LSTINQSLRTTK SPIVANHSR HSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD
Sbjct: 241 LSTINQSLRTTKGSPIVANHSRYHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300

Query: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL--- 360
           YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL   
Sbjct: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKVS 360

Query: 361 -----------------------------KRANVEALRREANFVREGAEREGIYRKEAET 420
                                        KRANVEALRREANFVREGAEREGIYRKEAET
Sbjct: 361 HLNKQRSKEAKKLEEINNKDVSGKEFAREKRANVEALRREANFVREGAEREGIYRKEAET 420

Query: 421 KAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHT 480
           KAV+DAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDL+IGMGA+GAVYKCNLHHT
Sbjct: 421 KAVQDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLIIGMGAHGAVYKCNLHHT 480

Query: 481 TVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL 540
           TVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL
Sbjct: 481 TVAVKVLHSRDDHKNTQFLRELEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL 540

Query: 541 YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG 600
           YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG
Sbjct: 541 YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG 600

Query: 601 LSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVV 660
           LST+LNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLT+KP V
Sbjct: 601 LSTVLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTAKPAV 660

Query: 661 ALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLL 712
           ALTHVVETA+E RSLIKVLDI+AGHWP EETYELARLGLWCAELQRKDRPNLKDQVLPLL
Sbjct: 661 ALTHVVETAVESRSLIKVLDIKAGHWPIEETYELARLGLWCAELQRKDRPNLKDQVLPLL 720

BLAST of Carg08500 vs. NCBI nr
Match: XP_022957881.1 (U-box domain-containing protein 35-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 690/816 (84.56%), Postives = 698/816 (85.54%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           MIMEGMEEEADKDLMLLP SSPAVAVAISGNKSSKHIIRWSLENFRHKGII         
Sbjct: 1   MIMEGMEEEADKDLMLLPSSSPAVAVAISGNKSSKHIIRWSLENFRHKGIISFKLIHIIP 60

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD
Sbjct: 61  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 121 DVANAIIEEVTRCSISKLVIGVSSHGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 180

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF S SPSRPLQRFQA
Sbjct: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFHSPSPSRPLQRFQA 240

Query: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300
           LSTINQSLRTTK SPIVANHSR HSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD
Sbjct: 241 LSTINQSLRTTKGSPIVANHSRYHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300

Query: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL--- 360
           YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL   
Sbjct: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKGV 360

Query: 361 ------------------------------------KRANVEALRREANFVREGAEREGI 420
                                               KRANVEALRREANFVREGAEREGI
Sbjct: 361 IAFDQVSHLNKQRSKEAKKLEEINNKDVSGKEFAREKRANVEALRREANFVREGAEREGI 420

Query: 421 YRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVY 480
           YRKEAETKAV+DAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDL+IGMGA+GAVY
Sbjct: 421 YRKEAETKAVQDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLIIGMGAHGAVY 480

Query: 481 KCNLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540
           KCNLHHTTVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN
Sbjct: 481 KCNLHHTTVAVKVLHSRDDHKNTQFLRELEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540

Query: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600
           GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV
Sbjct: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600

Query: 601 SKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660
           SKIGDVGLST+LNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL
Sbjct: 601 SKIGDVGLSTVLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660

Query: 661 LTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLK 712
           LT+KP VALTHVVETA+E RSLIKVLDI+AGHWP EETYELARLGLWCAELQRKDRPNLK
Sbjct: 661 LTAKPAVALTHVVETAVESRSLIKVLDIKAGHWPIEETYELARLGLWCAELQRKDRPNLK 720

BLAST of Carg08500 vs. NCBI nr
Match: XP_022957882.1 (U-box domain-containing protein 35-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 688/816 (84.31%), Postives = 696/816 (85.29%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           MIMEGMEEEADKDLMLLP SSPAVAVAISGNKSSKHIIRWSLENFRHKGII         
Sbjct: 1   MIMEGMEEEADKDLMLLPSSSPAVAVAISGNKSSKHIIRWSLENFRHKGIISFKLIHIIP 60

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRK  HDVVTLEAD
Sbjct: 61  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRK--HDVVTLEAD 120

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 121 DVANAIIEEVTRCSISKLVIGVSSHGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 180

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF S SPSRPLQRFQA
Sbjct: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFHSPSPSRPLQRFQA 240

Query: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300
           LSTINQSLRTTK SPIVANHSR HSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD
Sbjct: 241 LSTINQSLRTTKGSPIVANHSRYHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300

Query: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL--- 360
           YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL   
Sbjct: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKGV 360

Query: 361 ------------------------------------KRANVEALRREANFVREGAEREGI 420
                                               KRANVEALRREANFVREGAEREGI
Sbjct: 361 IAFDQVSHLNKQRSKEAKKLEEINNKDVSGKEFAREKRANVEALRREANFVREGAEREGI 420

Query: 421 YRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVY 480
           YRKEAETKAV+DAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDL+IGMGA+GAVY
Sbjct: 421 YRKEAETKAVQDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLIIGMGAHGAVY 480

Query: 481 KCNLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540
           KCNLHHTTVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN
Sbjct: 481 KCNLHHTTVAVKVLHSRDDHKNTQFLRELEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540

Query: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600
           GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV
Sbjct: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600

Query: 601 SKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660
           SKIGDVGLST+LNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL
Sbjct: 601 SKIGDVGLSTVLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660

Query: 661 LTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLK 712
           LT+KP VALTHVVETA+E RSLIKVLDI+AGHWP EETYELARLGLWCAELQRKDRPNLK
Sbjct: 661 LTAKPAVALTHVVETAVESRSLIKVLDIKAGHWPIEETYELARLGLWCAELQRKDRPNLK 720

BLAST of Carg08500 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 662.9 bits (1709), Expect = 4.0e-189
Identity = 390/826 (47.22%), Postives = 509/826 (61.62%), Query Frame = 0

Query: 18  PYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII-----------------MGNSIPVSQ 77
           P  S  V VA+SG+  SK+++ W++E F  +G +                 MGN+IP+S+
Sbjct: 16  PPPSRTVVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISE 75

Query: 78  VREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEVTR----- 137
           VR+DV  AYR+E+ WQ+ E L PY K+F +RKV  +V+ +E+D+VA AI EEVTR     
Sbjct: 76  VRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDR 135

Query: 138 ---------------------------YCTVYAISKGKLASIRPPDMETSVSIKDDSSEA 197
                                      +CTVY +SKGKL+ +RP D + + +I++D SE 
Sbjct: 136 IVIGGSSRSFFSRKADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSER 195

Query: 198 SSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQALSTINQSLRTTKSSPIVA 257
           +++ S SS  +S  +D  SS   S S  P   P R +Q F A++   Q+    ++S + +
Sbjct: 196 TNSSSGSSGPTSDSSDVMSSAHDSQSR-PLSLPVRRMQHFPAIA--GQASVPMETSSVGS 255

Query: 258 NHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSR-------------ASSCKSLPTDYK--- 317
           + +RC S+D EE  D   SS N S    T SR             A S  S   +Y    
Sbjct: 256 DETRCMSLDAEEARD--VSSINRS-STDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFG 315

Query: 318 ---SW-----------VPDEASS-SGTFNDYVSCESQADVNFELEKLRIELKHAREMYAI 377
              SW              +AS+ S   ++    ++Q ++NFE+EKLR EL+H +EMYA+
Sbjct: 316 TRFSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAV 375

Query: 378 VQQETANATLK--------------------------------RANVEALRREANFVREG 437
            Q ET +A+ K                                + N E  RR+A  +RE 
Sbjct: 376 AQTETFDASRKLGELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRER 435

Query: 438 AEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMG 497
           AERE   R+EAE K+  D KEK K E  L     QYQHF W++I++A SSFSE+L IGMG
Sbjct: 436 AEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMG 495

Query: 498 AYGAVYKCNLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLV 557
           AYGAVYKCNLHHTT  VK                LEILSKI HPHL+LLLGACP++  LV
Sbjct: 496 AYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALV 555

Query: 558 YEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANIL 617
           YEYMENGSLEDRL++  N+P +PW+ER+RIAWE+A+ALVFLH SKPKPIIHRDLKPANIL
Sbjct: 556 YEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANIL 615

Query: 618 LDRNLVSKIGDVGLSTMLNSDPLMS--TAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYA 677
           LD N VSK+GDVGLSTM+  DPL +  T +K +  VGTLCYIDPEYQRTG IS KSD+Y+
Sbjct: 616 LDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYS 675

Query: 678 FGMVILQLLTSKPVVALTHVVETAIEKR-SLIKVLDIEAGHWPTEETYELARLGLWCAEL 713
           FGM++LQLLT+KP +ALTH VE+A++     +K+LD +AG+WP EET ELA L L C EL
Sbjct: 676 FGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTEL 735

BLAST of Carg08500 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 6.4e-179
Identity = 377/836 (45.10%), Postives = 497/836 (59.45%), Query Frame = 0

Query: 14  LMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII-----------------MGNSI 73
           L L P  SP+VAVAI+G K SK+++ W+LE F  +G                   MG ++
Sbjct: 14  LSLPPPPSPSVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAV 73

Query: 74  PVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEE---- 133
            VS++REDV +AY++E+ W  +E L PYKKMF +RKVQ +V+ L++ + A AI EE    
Sbjct: 74  AVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGT 133

Query: 134 ----------------------------VTRYCTVYAISKGKLASIRPPDMETSVSIKDD 193
                                       V R+CTVY ISKGKLAS+RP + + S SI+ +
Sbjct: 134 GVTKLVIGMSLRGFFSRKIDMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFE 193

Query: 194 SSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSR-SPSRPLQRFQALSTINQSLRTTKS 253
            S ++S     S  S  L       +++ S   SR SP  P  +    S     + T+ S
Sbjct: 194 RSSSTS----GSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSS 253

Query: 254 SPIVANHSRCHSVDIEEPVDRLRSSPNVSYCA-----------------------QTLSR 313
                  S    ++I       + + + S+ A                       +   R
Sbjct: 254 GTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMR 313

Query: 314 ASSCKS------------LPTDYKSWVPDEAS--SSGTFNDYVSCESQADVNFELEKLRI 373
           +SS  +            +P +Y SWV   AS  S G  + +   ++Q ++NFE+EKLR 
Sbjct: 314 SSSSSNNHDLVNMDWGAVVPENY-SWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRA 373

Query: 374 ELKHAREMYAIVQQETANATLK--------------------------------RANVEA 433
           ELKH +EMYA+ Q ET  A+ K                                +   E 
Sbjct: 374 ELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEE 433

Query: 434 LRREANFVREGAEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAAS 493
             +EA  V+E   +E ++R+EAE KA  DA+EK K +++L     QYQH+ W++I +A S
Sbjct: 434 AMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATS 493

Query: 494 SFSEDLMIGMGAYGAVYKCNLHHTTVAVK----------------LEILSKIHHPHLLLL 553
            F+E+L IG+GAYG+VYKCNLHHTT AVK                LEILSKI HPHL+LL
Sbjct: 494 DFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLL 553

Query: 554 LGACPDKHCLVYEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPI 613
           LGACP++ CLVYEYM+NGSL+DRL    +TP IPW+ER+RIA E+ASALVFLH SKP+PI
Sbjct: 554 LGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPI 613

Query: 614 IHRDLKPANILLDRNLVSKIGDVGLSTMLNSDPLMS-TAFKDSGLVGTLCYIDPEYQRTG 673
           IHRDLKP NILLD N VSK+GDVGLSTM+N D + S T FK +  VGTLCYIDPEYQRTG
Sbjct: 614 IHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTG 673

Query: 674 LISPKSDVYAFGMVILQLLTSKPVVALTHVVETAI-EKRSLIKVLDIEAGHWPTEETYEL 713
           +ISPKSDVY+ G+VILQL+T+KP +A+TH+VE AI +    + +LD +AG WP  +T EL
Sbjct: 674 IISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTREL 733

BLAST of Carg08500 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 568.5 bits (1464), Expect = 1.0e-160
Identity = 351/809 (43.39%), Postives = 477/809 (58.96%), Query Frame = 0

Query: 24  VAVAISGNKS-SKHIIRWSLENFRHKGIIM--------GNSIPVSQVREDV-AAAYRKEM 83
           VAVAI GN S +K ++RW+L+ F  +  ++         +S  VS  R+D+  + Y+K++
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTTSVYKKDV 67

Query: 84  GWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEV-------------------- 143
             +T E L+P + MF  R+VQ D++ LE+DD+A+AI + V                    
Sbjct: 68  DRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFSW 127

Query: 144 ---------------TRYCTVYAISKGKLASIRPPDMETSVSIKDDSSEASSAHSYSSYS 203
                           R+C+V+ ISKGKL ++R  DM+T  SI DD SE       S +S
Sbjct: 128 KLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSE-------SRFS 187

Query: 204 SSSLTDSSSSLITSYSHFPSRSPSRPLQRFQALSTINQSLRTT----KSSPIVANHSRCH 263
           S    DS S  ++S S     S     QR QAL+T+NQ + T      + P   +H+R  
Sbjct: 188 S----DSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEPHHHHHNRAG 247

Query: 264 SVDIEEPVDRL--------RSSPNVSYCAQTLSRASSCKSLPTDYKSWVPDEASSSGTFN 323
           S+D++E   +L         SS  + Y    +S           ++S   +EASSS T++
Sbjct: 248 SLDVDE--SKLLNQKGFYRTSSSGIGYGGSDIS----------SWRSSQMEEASSSSTYS 307

Query: 324 DYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLK------RANVEALR---- 383
           D  S  SQ   +FELEKL+IEL+H + MYA+ Q E  +A+ K      R + EA R    
Sbjct: 308 DPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNL 367

Query: 384 ----------------------REANFVREGAEREGIYRKEAETKAVEDAKEKGKHESAL 443
                                  EA  VRE  ERE   R EAE +A E  KEK + E AL
Sbjct: 368 TIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDAL 427

Query: 444 HG---YLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVK-------- 503
            G     QQY  FEW++IV A SSFS++L IG+G YG+VY+CNLHHTTVAVK        
Sbjct: 428 EGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSS 487

Query: 504 --------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRLYRR------GNTP 563
                   LEILSKI HPHLLLLLGACP++  LVYEYM NGSLE+RL +R         P
Sbjct: 488 LTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPP 547

Query: 564 AIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVGLSTMLNS 623
            + W+ER+RIAWEIASAL FLH+++P+PI+HRDLKPANILLDRN VSKIGDVGLS M+N 
Sbjct: 548 PLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNL 607

Query: 624 DPL-MSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVVALTHVV 683
           DP   ST F ++G VGT  YIDPEYQRTG+++P+SD+YAFG+++LQL+T++  + L H +
Sbjct: 608 DPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSI 667

Query: 684 ETAIEKRS--LIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLLVRLR 712
           E A+  ++    ++LD  AG WP +E  E+  +GL CAE++++DRP+L  ++LP+L RL+
Sbjct: 668 EKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLK 727

BLAST of Carg08500 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 1.3e-155
Identity = 358/807 (44.36%), Postives = 471/807 (58.36%), Query Frame = 0

Query: 18  PYSSPAVAVAISGNKSSKHIIRWSLENFR------------HKGI-----IMGNSIPVSQ 77
           P     VA+AISG+  SK++I+W+L  F             H  I       GN + +S+
Sbjct: 28  PSEPMTVALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSISE 87

Query: 78  VREDVAAAYRKEMGWQTSEKLV-PYKKMFAQRKVQHD--------------VVTLEADDV 137
             E+VAAAYR+++  +T E L+ P+KKM  ++K++ D              +  LE++ V
Sbjct: 88  ELEEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNSV 147

Query: 138 ANAIIEEVTRY--------------------------------CTVYAISKGKLASIRPP 197
           A AI +EV ++                                CTVY +S G +  +   
Sbjct: 148 AVAITKEVNQHLISNLIIGRSSQAASSRNYDITASISASVSNLCTVYVVSNGGVHILAKD 207

Query: 198 DMETSVSIKDDSSEASSAHSYSSYSSSS--LTDSSSSLITSYSHFPSRSPSRPLQRFQAL 257
             +T  +  D S E+    + SS SS S   +D  S+ + S  H  S       +R Q L
Sbjct: 208 TSDTERN--DTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSN------KRMQNL 267

Query: 258 STINQSLRT-TKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 317
            TI + +    ++S   ++ ++  S D  E   + RSSP         SR+ S      D
Sbjct: 268 PTIVRGVSVPMETSSTESDETKKRSSDAAEEASK-RSSPET-------SRSVSWNPQFRD 327

Query: 318 YKSWVPDEASSSGTFN--------DYVSCESQADVNFELEKLRIELKHAREMYAIVQQET 377
           +       +S S  F          +   ++Q  +N E+ KLR EL+HA EMYA+ Q ET
Sbjct: 328 FDERKDAMSSMSSNFEYGNVVTPLGHYFTDNQDTLN-EISKLRAELRHAHEMYAVAQVET 387

Query: 378 ANAT-----LKRANVEALRREANFV----------REGAEREGIYRKEAETKAVEDAKEK 437
            +A+     LK   +  L  E   +          +   ERE   R+EAE KA  +AKEK
Sbjct: 388 LDASRKLNELKFEELTLLEHETKGIAKKETEKFEQKRREEREAAQRREAEMKATHEAKEK 447

Query: 438 GK-HESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVK--- 497
            K  ES+L     QYQ F W++I++A SSFSEDL IGMGAYG VYKCNLHHT  AVK   
Sbjct: 448 EKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLH 507

Query: 498 -------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRLYRRGNTPA 557
                        LEILSKI HPHL+LLLGACPD   LVYEYMENGSLEDRL++  ++  
Sbjct: 508 SAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQP 567

Query: 558 IPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVGLSTMLN-S 617
           IPW+ R RIAWE+ASALVFLH SKP PIIHRDLKPANILL+ N VSK+GDVGLSTM+  +
Sbjct: 568 IPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAA 627

Query: 618 DPLMS--TAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVVALTHV 677
           DPL +  T +K +  VGTLCYIDPEYQRTG ISPKSDVYAFGM+ILQLLT +  +ALT+ 
Sbjct: 628 DPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYT 687

Query: 678 VETAIEKRS---LIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLLVR 712
           VETA+E  +   LI++LD +AG+WP EET +LA L L C EL+ KDRP+L+DQ+LP+L  
Sbjct: 688 VETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLES 747

BLAST of Carg08500 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.4e-101
Identity = 263/756 (34.79%), Postives = 385/756 (50.93%), Query Frame = 0

Query: 50  IIMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKM----------FAQRKVQHDVVT 109
           +  G+ +PV +V E V   Y +++  +     VP+ KM          F  R+ +   V 
Sbjct: 87  VTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCKSTRSTKRYFRSRRTKGTGVP 146

Query: 110 LEADDVANAIIEEVTRYCTVYAISKGKLASIRPPDMETSVSIKDDSS--EASSAH----- 169
           L        ++      C VY + K ++ +     M+  ++ +  +S   A++AH     
Sbjct: 147 L-------TVLRYAPETCEVYIVCKDRITT---KSMDPLINREPCTSPHAAATAHDFLRD 206

Query: 170 ---SYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQALSTINQSLRTTKSSP---- 229
              S+ +  S +L D   S          RS S    RF+ALS      +T +SS     
Sbjct: 207 WAASFHTLRSPTLPDPRQSTEAG----TRRSASARELRFEALSLTCNKPKTPQSSKASSA 266

Query: 230 -----------------------------------IVANH-----------SRCHSVDIE 289
                                              IV+ H            +   V+IE
Sbjct: 267 TTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIVSEHRDSDRSPPETSRKSKKVEIE 326

Query: 290 EPVDRLRS---SPNVSY---CAQTLSRASSCKSLPTDYKSWVPDEASSSGTFNDYVSCES 349
           E V+RL++   S    Y   C +  S  +  K L T+Y        + S   N+ V  E 
Sbjct: 327 EEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTEY-------LNESKRVNNAVEKE- 386

Query: 350 QADVNFELEKLRIELKHAREMYAIVQQETANATLKRANVEALRREANFVREGAEREGIYR 409
                 EL++    L+  R M A+ + ETA A L                    RE   R
Sbjct: 387 ------ELQRNTAALEKERYMKAVKEVETAKALL-------------------AREFCQR 446

Query: 410 KEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKC 469
           + AE  A+    EK K    L G   +Y+ +  ++IV+A   FS + +IG G YG VY+C
Sbjct: 447 QIAEVNALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQC 506

Query: 470 NLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGS 529
           +L  T  AVK                +E+LS++ HPH++LLLGACP+  CLVYEY+ENGS
Sbjct: 507 SLDSTPAAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGS 566

Query: 530 LEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSK 589
           LE+ ++ R N P +PW+ R+R+ +E+A  L FLHSSKP+PI+HRDLKP NILL+RN VSK
Sbjct: 567 LEEYIFHRKNKPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSK 626

Query: 590 IGDVGLSTMLNS-DPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLL 649
           I DVGL+ ++    P   T +++S L GTL YIDPEY RTG I PKSD+YAFG++ILQLL
Sbjct: 627 IADVGLAKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLL 686

Query: 650 TSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKD 709
           T++    +   VE A++K +L ++LD     WP  ET ELAR+GL CAE + +DRP+LK 
Sbjct: 687 TARNPSGIVPAVENAVKKGTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKS 746

Query: 710 QVLPLLVRLRKVAD-EARNSASRVPPTIPNHFICPILMVVMNDPCVAADGYTYDRRAIEK 712
           +V+P+L RL + A+ + +   S +    P+H+ CPIL  +M +P +AADG+TY+R+AI  
Sbjct: 747 EVIPVLKRLVETANSKVKKEGSNL--RAPSHYFCPILREIMEEPEIAADGFTYERKAILA 793

BLAST of Carg08500 vs. ExPASy TrEMBL
Match: A0A6J1H1I8 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111459288 PE=4 SV=1)

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 690/809 (85.29%), Postives = 698/809 (86.28%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           MIMEGMEEEADKDLMLLP SSPAVAVAISGNKSSKHIIRWSLENFRHKGII         
Sbjct: 1   MIMEGMEEEADKDLMLLPSSSPAVAVAISGNKSSKHIIRWSLENFRHKGIISFKLIHIIP 60

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD
Sbjct: 61  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 121 DVANAIIEEVTRCSISKLVIGVSSHGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 180

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF S SPSRPLQRFQA
Sbjct: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFHSPSPSRPLQRFQA 240

Query: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300
           LSTINQSLRTTK SPIVANHSR HSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD
Sbjct: 241 LSTINQSLRTTKGSPIVANHSRYHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300

Query: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL--- 360
           YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL   
Sbjct: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKVS 360

Query: 361 -----------------------------KRANVEALRREANFVREGAEREGIYRKEAET 420
                                        KRANVEALRREANFVREGAEREGIYRKEAET
Sbjct: 361 HLNKQRSKEAKKLEEINNKDVSGKEFAREKRANVEALRREANFVREGAEREGIYRKEAET 420

Query: 421 KAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHT 480
           KAV+DAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDL+IGMGA+GAVYKCNLHHT
Sbjct: 421 KAVQDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLIIGMGAHGAVYKCNLHHT 480

Query: 481 TVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL 540
           TVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL
Sbjct: 481 TVAVKVLHSRDDHKNTQFLRELEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRL 540

Query: 541 YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG 600
           YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG
Sbjct: 541 YRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVG 600

Query: 601 LSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVV 660
           LST+LNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLT+KP V
Sbjct: 601 LSTVLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTAKPAV 660

Query: 661 ALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLL 712
           ALTHVVETA+E RSLIKVLDI+AGHWP EETYELARLGLWCAELQRKDRPNLKDQVLPLL
Sbjct: 661 ALTHVVETAVESRSLIKVLDIKAGHWPIEETYELARLGLWCAELQRKDRPNLKDQVLPLL 720

BLAST of Carg08500 vs. ExPASy TrEMBL
Match: A0A6J1H0G4 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111459288 PE=4 SV=1)

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 690/816 (84.56%), Postives = 698/816 (85.54%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           MIMEGMEEEADKDLMLLP SSPAVAVAISGNKSSKHIIRWSLENFRHKGII         
Sbjct: 1   MIMEGMEEEADKDLMLLPSSSPAVAVAISGNKSSKHIIRWSLENFRHKGIISFKLIHIIP 60

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD
Sbjct: 61  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 121 DVANAIIEEVTRCSISKLVIGVSSHGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 180

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF S SPSRPLQRFQA
Sbjct: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFHSPSPSRPLQRFQA 240

Query: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300
           LSTINQSLRTTK SPIVANHSR HSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD
Sbjct: 241 LSTINQSLRTTKGSPIVANHSRYHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300

Query: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL--- 360
           YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL   
Sbjct: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKGV 360

Query: 361 ------------------------------------KRANVEALRREANFVREGAEREGI 420
                                               KRANVEALRREANFVREGAEREGI
Sbjct: 361 IAFDQVSHLNKQRSKEAKKLEEINNKDVSGKEFAREKRANVEALRREANFVREGAEREGI 420

Query: 421 YRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVY 480
           YRKEAETKAV+DAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDL+IGMGA+GAVY
Sbjct: 421 YRKEAETKAVQDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLIIGMGAHGAVY 480

Query: 481 KCNLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540
           KCNLHHTTVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN
Sbjct: 481 KCNLHHTTVAVKVLHSRDDHKNTQFLRELEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540

Query: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600
           GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV
Sbjct: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600

Query: 601 SKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660
           SKIGDVGLST+LNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL
Sbjct: 601 SKIGDVGLSTVLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660

Query: 661 LTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLK 712
           LT+KP VALTHVVETA+E RSLIKVLDI+AGHWP EETYELARLGLWCAELQRKDRPNLK
Sbjct: 661 LTAKPAVALTHVVETAVESRSLIKVLDIKAGHWPIEETYELARLGLWCAELQRKDRPNLK 720

BLAST of Carg08500 vs. ExPASy TrEMBL
Match: A0A6J1H0D9 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111459288 PE=4 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 688/816 (84.31%), Postives = 696/816 (85.29%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           MIMEGMEEEADKDLMLLP SSPAVAVAISGNKSSKHIIRWSLENFRHKGII         
Sbjct: 1   MIMEGMEEEADKDLMLLPSSSPAVAVAISGNKSSKHIIRWSLENFRHKGIISFKLIHIIP 60

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRK  HDVVTLEAD
Sbjct: 61  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRK--HDVVTLEAD 120

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 121 DVANAIIEEVTRCSISKLVIGVSSHGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 180

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQA 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF S SPSRPLQRFQA
Sbjct: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHFHSPSPSRPLQRFQA 240

Query: 241 LSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300
           LSTINQSLRTTK SPIVANHSR HSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD
Sbjct: 241 LSTINQSLRTTKGSPIVANHSRYHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLPTD 300

Query: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL--- 360
           YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATL   
Sbjct: 301 YKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLKGV 360

Query: 361 ------------------------------------KRANVEALRREANFVREGAEREGI 420
                                               KRANVEALRREANFVREGAEREGI
Sbjct: 361 IAFDQVSHLNKQRSKEAKKLEEINNKDVSGKEFAREKRANVEALRREANFVREGAEREGI 420

Query: 421 YRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVY 480
           YRKEAETKAV+DAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDL+IGMGA+GAVY
Sbjct: 421 YRKEAETKAVQDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLIIGMGAHGAVY 480

Query: 481 KCNLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540
           KCNLHHTTVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMEN
Sbjct: 481 KCNLHHTTVAVKVLHSRDDHKNTQFLRELEILSKIHHPHLLLLLGACPDKHCLVYEYMEN 540

Query: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600
           GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV
Sbjct: 541 GSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLV 600

Query: 601 SKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660
           SKIGDVGLST+LNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL
Sbjct: 601 SKIGDVGLSTVLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQL 660

Query: 661 LTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLK 712
           LT+KP VALTHVVETA+E RSLIKVLDI+AGHWP EETYELARLGLWCAELQRKDRPNLK
Sbjct: 661 LTAKPAVALTHVVETAVESRSLIKVLDIKAGHWPIEETYELARLGLWCAELQRKDRPNLK 720

BLAST of Carg08500 vs. ExPASy TrEMBL
Match: A0A6J1K6L5 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111490674 PE=4 SV=1)

HSP 1 Score: 1250.7 bits (3235), Expect = 0.0e+00
Identity = 669/813 (82.29%), Postives = 684/813 (84.13%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           +IMEGMEEEADKDLMLLP SSP VAVAISGNK+SKHIIRWSLE F H+GII         
Sbjct: 10  IIMEGMEEEADKDLMLLPSSSPVVAVAISGNKNSKHIIRWSLEKFGHEGIISFKLLHIIP 69

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD
Sbjct: 70  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 129

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 130 DVANAIIEEVTRCSIRKLVIGISSQGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 189

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF--PSRSPSRPLQRF 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSS ITSYSHF  PS SPS+PLQRF
Sbjct: 190 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSFITSYSHFPSPSPSPSQPLQRF 249

Query: 241 QALSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLP 300
           QALSTINQ L TTK+SPIVANHSRCHSVDIEEPVD L SSPNVSY AQTLSRASSCKSLP
Sbjct: 250 QALSTINQCLLTTKTSPIVANHSRCHSVDIEEPVDCLGSSPNVSYSAQTLSRASSCKSLP 309

Query: 301 TDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLK 360
           TDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIEL+HAREM AI QQETANA+LK
Sbjct: 310 TDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELRHAREMNAISQQETANASLK 369

Query: 361 --------------------------------RANVEALRREANFVREGAEREGIYRKEA 420
                                           RANVEALRREA FVREGAE EGIYRKEA
Sbjct: 370 VRHLNKQRSKEAKKLEEMNNKEVSAKEFAREERANVEALRREAKFVREGAETEGIYRKEA 429

Query: 421 ETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLH 480
           ETKAV+DAKEKGKHESALHGYLQQYQHFEW+DIV AASSFSEDL IGMGA+GAVYKCNLH
Sbjct: 430 ETKAVQDAKEKGKHESALHGYLQQYQHFEWEDIVCAASSFSEDLRIGMGAHGAVYKCNLH 489

Query: 481 HTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLED 540
           HTTVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLED
Sbjct: 490 HTTVAVKVLHSRDDHKKTPFLQELEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLED 549

Query: 541 RLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGD 600
           RLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGD
Sbjct: 550 RLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGD 609

Query: 601 VGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKP 660
           VGLST+LNSDPLMSTAFKD G VGTLCYIDPEYQRTGLIS KSDVYAFGMVILQLLT+KP
Sbjct: 610 VGLSTVLNSDPLMSTAFKDGGPVGTLCYIDPEYQRTGLISRKSDVYAFGMVILQLLTAKP 669

Query: 661 VVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLP 714
            VALTHVVETAIEKRSLIKVLDIEAGHWP EETYELARLGLWCAELQRKDRPNLK+QVLP
Sbjct: 670 AVALTHVVETAIEKRSLIKVLDIEAGHWPIEETYELARLGLWCAELQRKDRPNLKEQVLP 729

BLAST of Carg08500 vs. ExPASy TrEMBL
Match: A0A6J1K0R0 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111490674 PE=4 SV=1)

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 669/820 (81.59%), Postives = 684/820 (83.41%), Query Frame = 0

Query: 1   MIMEGMEEEADKDLMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII--------- 60
           +IMEGMEEEADKDLMLLP SSP VAVAISGNK+SKHIIRWSLE F H+GII         
Sbjct: 6   IIMEGMEEEADKDLMLLPSSSPVVAVAISGNKNSKHIIRWSLEKFGHEGIISFKLLHIIP 65

Query: 61  --------MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 120
                   MGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD
Sbjct: 66  RITSVPTPMGNSIPVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEAD 125

Query: 121 DVANAIIEEVT---------------------------------RYCTVYAISKGKLASI 180
           DVANAIIEEVT                                 RYCTVYAISKGKLASI
Sbjct: 126 DVANAIIEEVTRCSIRKLVIGISSQGFFSRKLNGLSSRISAHAPRYCTVYAISKGKLASI 185

Query: 181 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSLITSYSHF--PSRSPSRPLQRF 240
           RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSS ITSYSHF  PS SPS+PLQRF
Sbjct: 186 RPPDMETSVSIKDDSSEASSAHSYSSYSSSSLTDSSSSFITSYSHFPSPSPSPSQPLQRF 245

Query: 241 QALSTINQSLRTTKSSPIVANHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSRASSCKSLP 300
           QALSTINQ L TTK+SPIVANHSRCHSVDIEEPVD L SSPNVSY AQTLSRASSCKSLP
Sbjct: 246 QALSTINQCLLTTKTSPIVANHSRCHSVDIEEPVDCLGSSPNVSYSAQTLSRASSCKSLP 305

Query: 301 TDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLK 360
           TDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIEL+HAREM AI QQETANA+LK
Sbjct: 306 TDYKSWVPDEASSSGTFNDYVSCESQADVNFELEKLRIELRHAREMNAISQQETANASLK 365

Query: 361 ---------------------------------------RANVEALRREANFVREGAERE 420
                                                  RANVEALRREA FVREGAE E
Sbjct: 366 GVIAFDQVRHLNKQRSKEAKKLEEMNNKEVSAKEFAREERANVEALRREAKFVREGAETE 425

Query: 421 GIYRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGA 480
           GIYRKEAETKAV+DAKEKGKHESALHGYLQQYQHFEW+DIV AASSFSEDL IGMGA+GA
Sbjct: 426 GIYRKEAETKAVQDAKEKGKHESALHGYLQQYQHFEWEDIVCAASSFSEDLRIGMGAHGA 485

Query: 481 VYKCNLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLVYEYM 540
           VYKCNLHHTTVAVK                LEILSKIHHPHLLLLLGACPDKHCLVYEYM
Sbjct: 486 VYKCNLHHTTVAVKVLHSRDDHKKTPFLQELEILSKIHHPHLLLLLGACPDKHCLVYEYM 545

Query: 541 ENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRN 600
           ENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRN
Sbjct: 546 ENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRN 605

Query: 601 LVSKIGDVGLSTMLNSDPLMSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVIL 660
           LVSKIGDVGLST+LNSDPLMSTAFKD G VGTLCYIDPEYQRTGLIS KSDVYAFGMVIL
Sbjct: 606 LVSKIGDVGLSTVLNSDPLMSTAFKDGGPVGTLCYIDPEYQRTGLISRKSDVYAFGMVIL 665

Query: 661 QLLTSKPVVALTHVVETAIEKRSLIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPN 714
           QLLT+KP VALTHVVETAIEKRSLIKVLDIEAGHWP EETYELARLGLWCAELQRKDRPN
Sbjct: 666 QLLTAKPAVALTHVVETAIEKRSLIKVLDIEAGHWPIEETYELARLGLWCAELQRKDRPN 725

BLAST of Carg08500 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 662.9 bits (1709), Expect = 2.9e-190
Identity = 390/826 (47.22%), Postives = 509/826 (61.62%), Query Frame = 0

Query: 18  PYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII-----------------MGNSIPVSQ 77
           P  S  V VA+SG+  SK+++ W++E F  +G +                 MGN+IP+S+
Sbjct: 16  PPPSRTVVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISE 75

Query: 78  VREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEVTR----- 137
           VR+DV  AYR+E+ WQ+ E L PY K+F +RKV  +V+ +E+D+VA AI EEVTR     
Sbjct: 76  VRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDR 135

Query: 138 ---------------------------YCTVYAISKGKLASIRPPDMETSVSIKDDSSEA 197
                                      +CTVY +SKGKL+ +RP D + + +I++D SE 
Sbjct: 136 IVIGGSSRSFFSRKADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSER 195

Query: 198 SSAHSYSSYSSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQALSTINQSLRTTKSSPIVA 257
           +++ S SS  +S  +D  SS   S S  P   P R +Q F A++   Q+    ++S + +
Sbjct: 196 TNSSSGSSGPTSDSSDVMSSAHDSQSR-PLSLPVRRMQHFPAIA--GQASVPMETSSVGS 255

Query: 258 NHSRCHSVDIEEPVDRLRSSPNVSYCAQTLSR-------------ASSCKSLPTDYK--- 317
           + +RC S+D EE  D   SS N S    T SR             A S  S   +Y    
Sbjct: 256 DETRCMSLDAEEARD--VSSINRS-STDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFG 315

Query: 318 ---SW-----------VPDEASS-SGTFNDYVSCESQADVNFELEKLRIELKHAREMYAI 377
              SW              +AS+ S   ++    ++Q ++NFE+EKLR EL+H +EMYA+
Sbjct: 316 TRFSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAV 375

Query: 378 VQQETANATLK--------------------------------RANVEALRREANFVREG 437
            Q ET +A+ K                                + N E  RR+A  +RE 
Sbjct: 376 AQTETFDASRKLGELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRER 435

Query: 438 AEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAASSFSEDLMIGMG 497
           AERE   R+EAE K+  D KEK K E  L     QYQHF W++I++A SSFSE+L IGMG
Sbjct: 436 AEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMG 495

Query: 498 AYGAVYKCNLHHTTVAVK----------------LEILSKIHHPHLLLLLGACPDKHCLV 557
           AYGAVYKCNLHHTT  VK                LEILSKI HPHL+LLLGACP++  LV
Sbjct: 496 AYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALV 555

Query: 558 YEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANIL 617
           YEYMENGSLEDRL++  N+P +PW+ER+RIAWE+A+ALVFLH SKPKPIIHRDLKPANIL
Sbjct: 556 YEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANIL 615

Query: 618 LDRNLVSKIGDVGLSTMLNSDPLMS--TAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYA 677
           LD N VSK+GDVGLSTM+  DPL +  T +K +  VGTLCYIDPEYQRTG IS KSD+Y+
Sbjct: 616 LDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYS 675

Query: 678 FGMVILQLLTSKPVVALTHVVETAIEKR-SLIKVLDIEAGHWPTEETYELARLGLWCAEL 713
           FGM++LQLLT+KP +ALTH VE+A++     +K+LD +AG+WP EET ELA L L C EL
Sbjct: 676 FGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTEL 735

BLAST of Carg08500 vs. TAIR 10
Match: AT5G61550.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 629.0 bits (1621), Expect = 4.6e-180
Identity = 377/836 (45.10%), Postives = 497/836 (59.45%), Query Frame = 0

Query: 14  LMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII-----------------MGNSI 73
           L L P  SP+VAVAI+G K SK+++ W+LE F  +G                   MG ++
Sbjct: 14  LSLPPPPSPSVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAV 73

Query: 74  PVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEE---- 133
            VS++REDV +AY++E+ W  +E L PYKKMF +RKVQ +V+ L++ + A AI EE    
Sbjct: 74  AVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGT 133

Query: 134 ----------------------------VTRYCTVYAISKGKLASIRPPDMETSVSIKDD 193
                                       V R+CTVY ISKGKLAS+RP + + S SI+ +
Sbjct: 134 GVTKLVIGMSLRGFFSRKIDMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFE 193

Query: 194 SSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSR-SPSRPLQRFQALSTINQSLRTTKS 253
            S ++S     S  S  L       +++ S   SR SP  P  +    S     + T+ S
Sbjct: 194 RSSSTS----GSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSS 253

Query: 254 SPIVANHSRCHSVDIEEPVDRLRSSPNVSYCA-----------------------QTLSR 313
                  S    ++I       + + + S+ A                       +   R
Sbjct: 254 GTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMR 313

Query: 314 ASSCKS------------LPTDYKSWVPDEAS--SSGTFNDYVSCESQADVNFELEKLRI 373
           +SS  +            +P +Y SWV   AS  S G  + +   ++Q ++NFE+EKLR 
Sbjct: 314 SSSSSNNHDLVNMDWGAVVPENY-SWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRA 373

Query: 374 ELKHAREMYAIVQQETANATLK--------------------------------RANVEA 433
           ELKH +EMYA+ Q ET  A+ K                                +   E 
Sbjct: 374 ELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEE 433

Query: 434 LRREANFVREGAEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAAS 493
             +EA  V+E   +E ++R+EAE KA  DA+EK K +++L     QYQH+ W++I +A S
Sbjct: 434 AMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATS 493

Query: 494 SFSEDLMIGMGAYGAVYKCNLHHTTVAVK----------------LEILSKIHHPHLLLL 553
            F+E+L IG+GAYG+VYKCNLHHTT AVK                LEILSKI HPHL+LL
Sbjct: 494 DFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLL 553

Query: 554 LGACPDKHCLVYEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPI 613
           LGACP++ CLVYEYM+NGSL+DRL    +TP IPW+ER+RIA E+ASALVFLH SKP+PI
Sbjct: 554 LGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPI 613

Query: 614 IHRDLKPANILLDRNLVSKIGDVGLSTMLNSDPLMS-TAFKDSGLVGTLCYIDPEYQRTG 673
           IHRDLKP NILLD N VSK+GDVGLSTM+N D + S T FK +  VGTLCYIDPEYQRTG
Sbjct: 614 IHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTG 673

Query: 674 LISPKSDVYAFGMVILQLLTSKPVVALTHVVETAI-EKRSLIKVLDIEAGHWPTEETYEL 713
           +ISPKSDVY+ G+VILQL+T+KP +A+TH+VE AI +    + +LD +AG WP  +T EL
Sbjct: 674 IISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTREL 733

BLAST of Carg08500 vs. TAIR 10
Match: AT5G61550.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 629.0 bits (1621), Expect = 4.6e-180
Identity = 377/836 (45.10%), Postives = 497/836 (59.45%), Query Frame = 0

Query: 14  LMLLPYSSPAVAVAISGNKSSKHIIRWSLENFRHKGII-----------------MGNSI 73
           L L P  SP+VAVAI+G K SK+++ W+LE F  +G                   MG ++
Sbjct: 14  LSLPPPPSPSVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAV 73

Query: 74  PVSQVREDVAAAYRKEMGWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEE---- 133
            VS++REDV +AY++E+ W  +E L PYKKMF +RKVQ +V+ L++ + A AI EE    
Sbjct: 74  AVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGT 133

Query: 134 ----------------------------VTRYCTVYAISKGKLASIRPPDMETSVSIKDD 193
                                       V R+CTVY ISKGKLAS+RP + + S SI+ +
Sbjct: 134 GVTKLVIGMSLRGFFSRKIDMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFE 193

Query: 194 SSEASSAHSYSSYSSSSLTDSSSSLITSYSHFPSR-SPSRPLQRFQALSTINQSLRTTKS 253
            S ++S     S  S  L       +++ S   SR SP  P  +    S     + T+ S
Sbjct: 194 RSSSTS----GSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSS 253

Query: 254 SPIVANHSRCHSVDIEEPVDRLRSSPNVSYCA-----------------------QTLSR 313
                  S    ++I       + + + S+ A                       +   R
Sbjct: 254 GTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMR 313

Query: 314 ASSCKS------------LPTDYKSWVPDEAS--SSGTFNDYVSCESQADVNFELEKLRI 373
           +SS  +            +P +Y SWV   AS  S G  + +   ++Q ++NFE+EKLR 
Sbjct: 314 SSSSSNNHDLVNMDWGAVVPENY-SWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRA 373

Query: 374 ELKHAREMYAIVQQETANATLK--------------------------------RANVEA 433
           ELKH +EMYA+ Q ET  A+ K                                +   E 
Sbjct: 374 ELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEE 433

Query: 434 LRREANFVREGAEREGIYRKEAETKAVEDAKEKGKHESALHGYLQQYQHFEWDDIVSAAS 493
             +EA  V+E   +E ++R+EAE KA  DA+EK K +++L     QYQH+ W++I +A S
Sbjct: 434 AMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATS 493

Query: 494 SFSEDLMIGMGAYGAVYKCNLHHTTVAVK----------------LEILSKIHHPHLLLL 553
            F+E+L IG+GAYG+VYKCNLHHTT AVK                LEILSKI HPHL+LL
Sbjct: 494 DFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLL 553

Query: 554 LGACPDKHCLVYEYMENGSLEDRLYRRGNTPAIPWYERYRIAWEIASALVFLHSSKPKPI 613
           LGACP++ CLVYEYM+NGSL+DRL    +TP IPW+ER+RIA E+ASALVFLH SKP+PI
Sbjct: 554 LGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPI 613

Query: 614 IHRDLKPANILLDRNLVSKIGDVGLSTMLNSDPLMS-TAFKDSGLVGTLCYIDPEYQRTG 673
           IHRDLKP NILLD N VSK+GDVGLSTM+N D + S T FK +  VGTLCYIDPEYQRTG
Sbjct: 614 IHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTG 673

Query: 674 LISPKSDVYAFGMVILQLLTSKPVVALTHVVETAI-EKRSLIKVLDIEAGHWPTEETYEL 713
           +ISPKSDVY+ G+VILQL+T+KP +A+TH+VE AI +    + +LD +AG WP  +T EL
Sbjct: 674 IISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTREL 733

BLAST of Carg08500 vs. TAIR 10
Match: AT5G61560.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 568.5 bits (1464), Expect = 7.3e-162
Identity = 351/809 (43.39%), Postives = 477/809 (58.96%), Query Frame = 0

Query: 24  VAVAISGNKS-SKHIIRWSLENFRHKGIIM--------GNSIPVSQVREDV-AAAYRKEM 83
           VAVAI GN S +K ++RW+L+ F  +  ++         +S  VS  R+D+  + Y+K++
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTTSVYKKDV 67

Query: 84  GWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEV-------------------- 143
             +T E L+P + MF  R+VQ D++ LE+DD+A+AI + V                    
Sbjct: 68  DRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFSW 127

Query: 144 ---------------TRYCTVYAISKGKLASIRPPDMETSVSIKDDSSEASSAHSYSSYS 203
                           R+C+V+ ISKGKL ++R  DM+T  SI DD SE       S +S
Sbjct: 128 KLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSE-------SRFS 187

Query: 204 SSSLTDSSSSLITSYSHFPSRSPSRPLQRFQALSTINQSLRTT----KSSPIVANHSRCH 263
           S    DS S  ++S S     S     QR QAL+T+NQ + T      + P   +H+R  
Sbjct: 188 S----DSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEPHHHHHNRAG 247

Query: 264 SVDIEEPVDRL--------RSSPNVSYCAQTLSRASSCKSLPTDYKSWVPDEASSSGTFN 323
           S+D++E   +L         SS  + Y    +S           ++S   +EASSS T++
Sbjct: 248 SLDVDE--SKLLNQKGFYRTSSSGIGYGGSDIS----------SWRSSQMEEASSSSTYS 307

Query: 324 DYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLK------RANVEALR---- 383
           D  S  SQ   +FELEKL+IEL+H + MYA+ Q E  +A+ K      R + EA R    
Sbjct: 308 DPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNL 367

Query: 384 ----------------------REANFVREGAEREGIYRKEAETKAVEDAKEKGKHESAL 443
                                  EA  VRE  ERE   R EAE +A E  KEK + E AL
Sbjct: 368 TIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDAL 427

Query: 444 HG---YLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVK-------- 503
            G     QQY  FEW++IV A SSFS++L IG+G YG+VY+CNLHHTTVAVK        
Sbjct: 428 EGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSS 487

Query: 504 --------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRLYRR------GNTP 563
                   LEILSKI HPHLLLLLGACP++  LVYEYM NGSLE+RL +R         P
Sbjct: 488 LTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPP 547

Query: 564 AIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVGLSTMLNS 623
            + W+ER+RIAWEIASAL FLH+++P+PI+HRDLKPANILLDRN VSKIGDVGLS M+N 
Sbjct: 548 PLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNL 607

Query: 624 DPL-MSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVVALTHVV 683
           DP   ST F ++G VGT  YIDPEYQRTG+++P+SD+YAFG+++LQL+T++  + L H +
Sbjct: 608 DPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSI 667

Query: 684 ETAIEKRS--LIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLLVRLR 712
           E A+  ++    ++LD  AG WP +E  E+  +GL CAE++++DRP+L  ++LP+L RL+
Sbjct: 668 EKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLK 727

BLAST of Carg08500 vs. TAIR 10
Match: AT5G61560.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 567.4 bits (1461), Expect = 1.6e-161
Identity = 351/810 (43.33%), Postives = 480/810 (59.26%), Query Frame = 0

Query: 24  VAVAISGNKS-SKHIIRWSLENFRHKGIIM--------GNSIPVSQVREDV-AAAYRKEM 83
           VAVAI GN S +K ++RW+L+ F  +  ++         +S  VS  R+D+  + Y+K++
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTTSVYKKDV 67

Query: 84  GWQTSEKLVPYKKMFAQRKVQHDVVTLEADDVANAIIEEV-------------------- 143
             +T E L+P + MF  R+VQ D++ LE+DD+A+AI + V                    
Sbjct: 68  DRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFSW 127

Query: 144 ---------------TRYCTVYAISKGKLASIRPPDMETSVSIKDDSSEAS-SAHSYSSY 203
                           R+C+V+ ISKGKL ++R  DM+T  SI DD SE+  S+ S+S  
Sbjct: 128 KLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSESRFSSDSHSVS 187

Query: 204 SSSSLTDSSSSLITSYSHFPSRSPSRPLQRFQALSTINQSLRTT----KSSPIVANHSRC 263
           S+SS   SS+ L+               QR QAL+T+NQ + T      + P   +H+R 
Sbjct: 188 STSSHQFSSTPLL--------------FQRIQALTTVNQKVGTNIGKQNNEPHHHHHNRA 247

Query: 264 HSVDIEEPVDRL--------RSSPNVSYCAQTLSRASSCKSLPTDYKSWVPDEASSSGTF 323
            S+D++E   +L         SS  + Y    +S           ++S   +EASSS T+
Sbjct: 248 GSLDVDE--SKLLNQKGFYRTSSSGIGYGGSDIS----------SWRSSQMEEASSSSTY 307

Query: 324 NDYVSCESQADVNFELEKLRIELKHAREMYAIVQQETANATLK------RANVEALR--- 383
           +D  S  SQ   +FELEKL+IEL+H + MYA+ Q E  +A+ K      R + EA R   
Sbjct: 308 SDPTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKN 367

Query: 384 -----------------------REANFVREGAEREGIYRKEAETKAVEDAKEKGKHESA 443
                                   EA  VRE  ERE   R EAE +A E  KEK + E A
Sbjct: 368 LTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDA 427

Query: 444 LHG---YLQQYQHFEWDDIVSAASSFSEDLMIGMGAYGAVYKCNLHHTTVAVK------- 503
           L G     QQY  FEW++IV A SSFS++L IG+G YG+VY+CNLHHTTVAVK       
Sbjct: 428 LEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKS 487

Query: 504 ---------LEILSKIHHPHLLLLLGACPDKHCLVYEYMENGSLEDRLYRR------GNT 563
                    LEILSKI HPHLLLLLGACP++  LVYEYM NGSLE+RL +R         
Sbjct: 488 SLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQP 547

Query: 564 PAIPWYERYRIAWEIASALVFLHSSKPKPIIHRDLKPANILLDRNLVSKIGDVGLSTMLN 623
           P + W+ER+RIAWEIASAL FLH+++P+PI+HRDLKPANILLDRN VSKIGDVGLS M+N
Sbjct: 548 PPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVN 607

Query: 624 SDPL-MSTAFKDSGLVGTLCYIDPEYQRTGLISPKSDVYAFGMVILQLLTSKPVVALTHV 683
            DP   ST F ++G VGT  YIDPEYQRTG+++P+SD+YAFG+++LQL+T++  + L H 
Sbjct: 608 LDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHS 667

Query: 684 VETAIEKRS--LIKVLDIEAGHWPTEETYELARLGLWCAELQRKDRPNLKDQVLPLLVRL 712
           +E A+  ++    ++LD  AG WP +E  E+  +GL CAE++++DRP+L  ++LP+L RL
Sbjct: 668 IEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERL 727

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035547.10.0e+00100.00U-box domain-containing protein 35 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6605637.10.0e+0087.42U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022957883.10.0e+0085.29U-box domain-containing protein 35-like isoform X3 [Cucurbita moschata][more]
XP_022957881.10.0e+0084.56U-box domain-containing protein 35-like isoform X1 [Cucurbita moschata][more]
XP_022957882.10.0e+0084.31U-box domain-containing protein 35-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SW114.0e-18947.22U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2... [more]
Q9FKG66.4e-17945.10U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2... [more]
Q9FKG51.0e-16043.39U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2... [more]
Q9LU471.3e-15544.36Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q8S8S75.4e-10134.79U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1H1I80.0e+0085.29E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111459288 PE=4 SV=1[more]
A0A6J1H0G40.0e+0084.56E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111459288 PE=4 SV=1[more]
A0A6J1H0D90.0e+0084.31E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111459288 PE=4 SV=1[more]
A0A6J1K6L50.0e+0082.29E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111490674 PE=4 SV=1[more]
A0A6J1K0R00.0e+0081.59E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111490674 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G25160.12.9e-19047.22U-box domain-containing protein kinase family protein [more]
AT5G61550.14.6e-18045.10U-box domain-containing protein kinase family protein [more]
AT5G61550.24.6e-18045.10U-box domain-containing protein kinase family protein [more]
AT5G61560.17.3e-16243.39U-box domain-containing protein kinase family protein [more]
AT5G61560.21.6e-16143.33U-box domain-containing protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 281..318
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 437..639
e-value: 2.2E-46
score: 159.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 404..436
e-value: 3.2E-7
score: 32.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 355..403
e-value: 2.7E-7
score: 32.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..163
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 114..311
coord: 20..115
coord: 309..709
NoneNo IPR availablePANTHERPTHR45647:SF15U-BOX DOMAIN-CONTAINING PROTEIN 35coord: 114..311
coord: 20..115
NoneNo IPR availablePANTHERPTHR45647:SF15U-BOX DOMAIN-CONTAINING PROTEIN 35coord: 309..709
NoneNo IPR availableCDDcd16655RING-Ubox_WDSUB1_likecoord: 647..687
e-value: 1.80586E-18
score: 77.2591
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 631..711
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 376..622
e-value: 5.0E-17
score: 72.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 398..615
e-value: 6.8E-31
score: 107.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 376..622
score: 28.951391
IPR003613U box domainSMARTSM00504Ubox_2coord: 646..708
e-value: 6.0E-25
score: 98.9
IPR003613U box domainPFAMPF04564U-boxcoord: 643..712
e-value: 2.6E-16
score: 59.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 642..713
score: 30.289442
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 642..713
e-value: 9.9E-26
score: 91.5
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 382..403
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 478..490
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 356..616

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08500-RACarg08500-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0004672 protein kinase activity