Carg08499 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08499
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein kinase domain-containing protein
LocationCarg_Chr02: 5361186 .. 5363227 (-)
RNA-Seq ExpressionCarg08499
SyntenyCarg08499
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCAAATTAATCCTTATAGTCGCTTCCGCCACCATTTTTGTTCTTCTTCTAGTTTCCATCTTCTTTTTCCGCCATAAGCTCTCCAAAACAAACCACAGACACGACATCGAAAACCCATCGCCGGACAACAACCAGAAAAAACAAGACACGGCGGCAGCGACCGTGGAGGAGCTAACGACGTTTGAAGGCGGCGAAGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGTGAAGTGATCGGAAAATACCACTATGGAACTCTGTACAAGGCTTCCTTACAGAGTACCCAGAGAACTACACTACTCATGTTCCTCCGCCCTGCCGCCGTGGAATCTAGTGTAGACGACGTCGTTCGGTTTCTGGGTTCGATCCGGCACCGGAATTTGGTGCCGATGTTGGGATTTTACGCCGGCGGGAGAGGCGAGAGACTTCTGGTTCATCCGTTTTATGGCAGAGGGAATCTGTCAGAGTTCATCACAGGTAAGTTTCATTTTGATTTTCTGTTTGTTTCTCGAGAATGTGAAAAGGGAAAAGAATCTGAGGGGAAATTGAGTGAATATCGGGGCAATGTCGGTAACCCTTTTCGCTTTCGGTTGCTGTCGGTTTTCCATGGGCGAATCAGAGAAGACGACGATTCCGGTTCGGCCGGCGGGCACGTTTTTTATTTGTCTTTCCTACCATATATATTTTTTAAAAAAATATTAAATTTAAACCAAAATTTTAATAGGCATTTTAAAAAATTAGAGATTAAAATATAAAATGAAAAATATCTAAAATTTTATTAAATGTTGAAGGAAAATAATTTTTTTAATGCATTATTGAGGTTGAAATTAAGATAATAAAATTAAAAGAAAAATTAAAAATTCTGTCCACCTCATATATTAGCTATAGCATTGATATTTTTTCTTTAGATTTTTTTCTGTATTTATTTATTTATTTATTTCTCTTTGTTAACATATTCAACTTTAATTCTCATATTATCTATGTTTTATTTATTTATTTATTTATATTTAAAATGTAAATTTTGTAGGTCATCTAAACATTTTAAATTAGTGGATAATAATTATTTTATTTATTTTTTTATTTTCAGTATTTATTTATAAAAAATTTATATTTTAGAAATCCCTAAAAATTTTCTAAACAATCATTTGTATTCCAATTGTGCTGTTTCTAAAAATTTTCTAAAAATTTATATTTCATTTAAAACTTTATTATACGTTAAATTGGAGAATATATTCCTCTTCTTTATTTATTTTTCTTTTTTTGAAATTAAATAGGAAATATATTCCAATTGTGCTGTTTGTAAATGGAGAATATATATATATATATATTTTTTATAAATAAAAGGAAATTAACCTTAAAAGACAATGTTTAAGATTTAATTTATTGAAAATATCGGAAAGTAAAATTTAAATAAAATAAACCGATTTTTAAAAATTATTAAATAAATATTTATTTTAAGAAGGGGAAAAGCTAAGGTACAACAGCAGTTATGATAATTTCTTGTGATTGAACAGTGGGAGAATCGGAATCAAAAAATTGGGAAATCATCTACAAAATCTCTCTCGGAATCGCGAAAGCCCTAGATCATCTCCACCATGGATTACCAAAACCAGTACCGCACGGAAATCTCAAGCCCAAGAACATTTTTCTCGATCCAGATTTCGAGCCGTACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAATTGCTCCAAGCTTCCTCCGCCTCCGGCTACAACGCGCCGGAACTGGTCAGGATGACTGACGCCGACGAACGGACCGACGTCTACAGCTACGGCAAGATCTTACTCGAGTTGCTCTCCGCCAAAGATACAATGAATCTAACTGCGGTTGCTTGTGGCGGAGACGGCGGCGATTGCAGTGTTTCGAAAGAGCGGAATCTCCAATGCCTTTTTCAGATCGGCGAGGCTTGTTGTTGTTCTTCGCCGATTCGGCGGCCGGATTTCAAGGAAGTTATTGAGAAGATTGAAGAAATCGGAAGGATTGAGATGGATTGA

mRNA sequence

ATGCTCAAATTAATCCTTATAGTCGCTTCCGCCACCATTTTTGTTCTTCTTCTAGTTTCCATCTTCTTTTTCCGCCATAAGCTCTCCAAAACAAACCACAGACACGACATCGAAAACCCATCGCCGGACAACAACCAGAAAAAACAAGACACGGCGGCAGCGACCGTGGAGGAGCTAACGACGTTTGAAGGCGGCGAAGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGTGAAGTGATCGGAAAATACCACTATGGAACTCTGTACAAGGCTTCCTTACAGAGTACCCAGAGAACTACACTACTCATGTTCCTCCGCCCTGCCGCCGTGGAATCTAGTGTAGACGACGTCGTTCGGTTTCTGGGTTCGATCCGGCACCGGAATTTGGTGCCGATGTTGGGATTTTACGCCGGCGGGAGAGGCGAGAGACTTCTGGTTCATCCGTTTTATGGCAGAGGGAATCTGTCAGAGTTCATCACAGTGGGAGAATCGGAATCAAAAAATTGGGAAATCATCTACAAAATCTCTCTCGGAATCGCGAAAGCCCTAGATCATCTCCACCATGGATTACCAAAACCAGTACCGCACGGAAATCTCAAGCCCAAGAACATTTTTCTCGATCCAGATTTCGAGCCGTACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAATTGCTCCAAGCTTCCTCCGCCTCCGGCTACAACGCGCCGGAACTGGTCAGGATGACTGACGCCGACGAACGGACCGACGTCTACAGCTACGGCAAGATCTTACTCGAGTTGCTCTCCGCCAAAGATACAATGAATCTAACTGCGGTTGCTTGTGGCGGAGACGGCGGCGATTGCAGTGTTTCGAAAGAGCGGAATCTCCAATGCCTTTTTCAGATCGGCGAGGCTTGTTGTTGTTCTTCGCCGATTCGGCGGCCGGATTTCAAGGAAGTTATTGAGAAGATTGAAGAAATCGGAAGGATTGAGATGGATTGA

Coding sequence (CDS)

ATGCTCAAATTAATCCTTATAGTCGCTTCCGCCACCATTTTTGTTCTTCTTCTAGTTTCCATCTTCTTTTTCCGCCATAAGCTCTCCAAAACAAACCACAGACACGACATCGAAAACCCATCGCCGGACAACAACCAGAAAAAACAAGACACGGCGGCAGCGACCGTGGAGGAGCTAACGACGTTTGAAGGCGGCGAAGATTTGACGATCGACGAGATTCTAGAAGCTCCGGGTGAAGTGATCGGAAAATACCACTATGGAACTCTGTACAAGGCTTCCTTACAGAGTACCCAGAGAACTACACTACTCATGTTCCTCCGCCCTGCCGCCGTGGAATCTAGTGTAGACGACGTCGTTCGGTTTCTGGGTTCGATCCGGCACCGGAATTTGGTGCCGATGTTGGGATTTTACGCCGGCGGGAGAGGCGAGAGACTTCTGGTTCATCCGTTTTATGGCAGAGGGAATCTGTCAGAGTTCATCACAGTGGGAGAATCGGAATCAAAAAATTGGGAAATCATCTACAAAATCTCTCTCGGAATCGCGAAAGCCCTAGATCATCTCCACCATGGATTACCAAAACCAGTACCGCACGGAAATCTCAAGCCCAAGAACATTTTTCTCGATCCAGATTTCGAGCCGTACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAACGCCGCCGCAACTCGAGAATTGCTCCAAGCTTCCTCCGCCTCCGGCTACAACGCGCCGGAACTGGTCAGGATGACTGACGCCGACGAACGGACCGACGTCTACAGCTACGGCAAGATCTTACTCGAGTTGCTCTCCGCCAAAGATACAATGAATCTAACTGCGGTTGCTTGTGGCGGAGACGGCGGCGATTGCAGTGTTTCGAAAGAGCGGAATCTCCAATGCCTTTTTCAGATCGGCGAGGCTTGTTGTTGTTCTTCGCCGATTCGGCGGCCGGATTTCAAGGAAGTTATTGAGAAGATTGAAGAAATCGGAAGGATTGAGATGGATTGA

Protein sequence

MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD
Homology
BLAST of Carg08499 vs. NCBI nr
Match: KAG6605638.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035548.1 putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 664.5 bits (1713), Expect = 4.9e-187
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499 vs. NCBI nr
Match: XP_022957896.1 (putative kinase-like protein TMKL1 [Cucurbita moschata])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 331/334 (99.10%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLLVSIFF RHKLSKTNHR+DIENPSPDNNQKKQDTAAATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFLRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACC SSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499 vs. NCBI nr
Match: XP_023532398.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.0 bits (1678), Expect = 5.7e-183
Identity = 329/334 (98.50%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHR+DIENPSPDNNQKKQDTAAATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPK VPHGNLKPKNIFLDP+FEP+VSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKSVPHGNLKPKNIFLDPNFEPHVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACC SSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499 vs. NCBI nr
Match: XP_022994989.1 (putative kinase-like protein TMKL1 [Cucurbita maxima])

HSP 1 Score: 647.9 bits (1670), Expect = 4.8e-182
Identity = 324/334 (97.01%), Postives = 330/334 (98.80%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLL+SIFFFRHKLSK+NHRHDIENPSPDNNQKKQDT AATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLLSIFFFRHKLSKSNHRHDIENPSPDNNQKKQDTTAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TF+GGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQR TLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFQGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRATLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPKPVPHGNLKP NIFLDP+FEPYVSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKPVPHGNLKPMNIFLDPNFEPYVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELV+MTDADERTDVYSYGKILLELLSAK+TMN TAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVKMTDADERTDVYSYGKILLELLSAKETMNQTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499 vs. NCBI nr
Match: XP_023520536.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550283.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550284.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491.5 bits (1264), Expect = 5.7e-135
Identity = 256/336 (76.19%), Postives = 278/336 (82.74%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSI--FFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEE 60
           MLK IL+VASA IFVLLL+SI  FFFRHKLS T HRHD+E P  D + KK+ T   +   
Sbjct: 48  MLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 107

Query: 61  LTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDV 120
           L TFEGGEDLTIDEILEAPGEVIGK HYGTLYKASLQSTQ T LL FLRP A      DV
Sbjct: 108 LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEY-DV 167

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG+GNL+E I   ESESKNWE+I KISL
Sbjct: 168 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 227

Query: 181 GIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSA 240
           GIA+ALDHLH+GL KP+PHGNLK KNI LD +FEP+VSDFGLHLLLNAAATRE+LQ S+A
Sbjct: 228 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSAA 287

Query: 241 SGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQ 300
           SGY APEL+ M+DADERTDV+SYGKILLELLSAK+T        GGDGGDC VS+ER L+
Sbjct: 288 SGYKAPELINMSDADERTDVFSYGKILLELLSAKET--------GGDGGDCGVSEERTLR 347

Query: 301 CLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           CL QIGEACC SSP  RPDFKEVI KI EIGRIEMD
Sbjct: 348 CLLQIGEACCSSSPDLRPDFKEVIAKITEIGRIEMD 374

BLAST of Carg08499 vs. ExPASy Swiss-Prot
Match: P33543 (Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.8e-39
Identity = 107/350 (30.57%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 4   LILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFE 63
           +I +++ A +   LL+     + + S      D+E    ++   +++       +L  F+
Sbjct: 305 VIGLMSGAVVVASLLIGYLQNKKRKSSIESEDDLEEGDEEDEIGEKEGGEG---KLVVFQ 364

Query: 64  GGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE-SSVDDVVRFL 123
           GGE+LT+D++L A G+V+ K  YGT+YKA L       L +       + SS   V+R L
Sbjct: 365 GGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNIALRLLREGTCKDRSSCLPVIRQL 424

Query: 124 GSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFI--TVGESESKNWEIIYKISLGI 183
           G IRH NLVP+  FY G RGE+LL++ +    +L + +  +     + NW   +KI+LGI
Sbjct: 425 GRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIALGI 484

Query: 184 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 243
           A+ L +LH G   P+ HGN++ KN+ +D  F   +++FGL  ++  A   E++  + + G
Sbjct: 485 ARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKSDG 544

Query: 244 YNAPELVRMTDADERTDVYSYGKILLELLSAK-------------DTMNLTAVACGGDGG 303
           Y APEL +M   + R+DVY++G +LLE+L  K             D  +L   A   +  
Sbjct: 545 YKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLVKAAVLEETT 604

Query: 304 ----DCSVSK------ERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEE 328
               D    K      E  L    ++   CC      RP  +EV++++EE
Sbjct: 605 MEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

BLAST of Carg08499 vs. ExPASy Swiss-Prot
Match: C0LGP9 (Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis thaliana OX=3702 GN=IMK3 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 9.5e-32
Identity = 90/281 (32.03%), Postives = 149/281 (53.02%), Query Frame = 0

Query: 3   KLILIVASATIFVLLLVSI----FFFRHKLSKTNHRHDIENP----SPDNNQKKQDTAAA 62
           K I+++AS  + +++L+ +       R K ++T  +     P    +      + +    
Sbjct: 408 KDIILIASGALLIVMLILVCVLCCLLRKKANETKAKGGEAGPGAVAAKTEKGGEAEAGGE 467

Query: 63  TVEELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVES- 122
           T  +L  F+G    T D++L A  E++GK  YGT+YKA+L+   +  +   LR    +S 
Sbjct: 468 TGGKLVHFDGPMAFTADDLLCATAEIMGKSTYGTVYKATLEDGSQVAVKR-LREKITKSQ 527

Query: 123 -SVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITV-GESESKNWE 182
              ++ +  LG IRH NL+ +  +Y G +GE+L+V  +  RG+L+ F+   G     NW 
Sbjct: 528 KEFENEINVLGRIRHPNLLALRAYYLGPKGEKLVVFDYMSRGSLATFLHARGPDVHINWP 587

Query: 183 IIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRE 242
               +  G+A+ L +LH      + HGNL   N+ LD +    +SD+GL  L+ AAA   
Sbjct: 588 TRMSLIKGMARGLFYLH--THANIIHGNLTSSNVLLDENITAKISDYGLSRLMTAAAGSS 647

Query: 243 LLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAK 273
           ++  + A GY APEL ++  A+ +TDVYS G I+LELL+ K
Sbjct: 648 VIATAGALGYRAPELSKLKKANTKTDVYSLGVIILELLTGK 685

BLAST of Carg08499 vs. ExPASy Swiss-Prot
Match: Q9SCT4 (Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabidopsis thaliana OX=3702 GN=IMK2 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-31
Identity = 104/353 (29.46%), Postives = 164/353 (46.46%), Query Frame = 0

Query: 4   LILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVE------ 63
           +ILI   A + +LLL+        + K       +     + +      A T        
Sbjct: 459 VILIAIGALLAILLLLCCILLCCLIKKRAALKQKDGKDKTSEKTVSAGVAGTASAGGEMG 518

Query: 64  -ELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE--SS 123
            +L  F+G    T D++L A  E++GK  YGT YKA+L+      +   LR    +    
Sbjct: 519 GKLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKR-LREKTTKGVKE 578

Query: 124 VDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITV-GESESKNWEII 183
            +  V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+LS F+   G      WE  
Sbjct: 579 FEGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETR 638

Query: 184 YKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELL 243
            KI+ GI++ L HLH    + + H NL   NI LD     +++D+GL  L+ AAA   ++
Sbjct: 639 MKIAKGISRGLAHLHSN--ENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVI 698

Query: 244 QASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKD-------------TMNLTAV 303
             +   GY APE  ++ +A  +TDVYS G I+LELL+ K                ++   
Sbjct: 699 ATAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKE 758

Query: 304 ACGGDGGDCSVSKER-----NLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
               +  D  + +E       L    ++   C   SP  RP+  +V+E++EEI
Sbjct: 759 EWTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

BLAST of Carg08499 vs. ExPASy Swiss-Prot
Match: Q9S7I6 (LRR receptor-like serine/threonine-protein kinase RPK2 OS=Arabidopsis thaliana OX=3702 GN=RPK2 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 104/350 (29.71%), Postives = 160/350 (45.71%), Query Frame = 0

Query: 9    ASATIFVLL-LVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTF-EGGE 68
            ASA + VL+ LV +FF+  K                    K    A T  E+T F + G 
Sbjct: 818  ASAIVSVLIALVILFFYTRKW-----------------HPKSKIMATTKREVTMFMDIGV 877

Query: 69   DLTIDEILEAPG-----EVIGKYHYGTLYKASLQSTQRTTL--LMFLRPAAVESSVDDVV 128
             +T D ++ A G      +IG   +G  YKA +       +  L   R   V+    + +
Sbjct: 878  PITFDNVVRATGNFNASNLIGNGGFGATYKAEISQDVVVAIKRLSIGRFQGVQQFHAE-I 937

Query: 129  RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLG 188
            + LG +RH NLV ++G++A    E  LV+ +   GNL +FI   E  +++W +++KI+L 
Sbjct: 938  KTLGRLRHPNLVTLIGYHA-SETEMFLVYNYLPGGNLEKFIQ--ERSTRDWRVLHKIALD 997

Query: 189  IAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSAS 248
            IA+AL +LH      V H ++KP NI LD D   Y+SDFGL  LL  + T      +   
Sbjct: 998  IARALAYLHDQCVPRVLHRDVKPSNILLDDDCNAYLSDFGLARLLGTSETHATTGVAGTF 1057

Query: 249  GYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGG--------------- 308
            GY APE        ++ DVYSYG +LLELLS K  ++ + V+ G                
Sbjct: 1058 GYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKALDPSFVSYGNGFNIVQWACMLLRQG 1117

Query: 309  ------DGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
                    G        +L  +  +   C   S   RP  K+V+ +++++
Sbjct: 1118 RAKEFFTAGLWDAGPHDDLVEVLHLAVVCTVDSLSTRPTMKQVVRRLKQL 1146

BLAST of Carg08499 vs. ExPASy Swiss-Prot
Match: Q9M2Z1 (Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM2 OS=Arabidopsis thaliana OX=3702 GN=BAM2 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.5e-29
Identity = 94/314 (29.94%), Postives = 148/314 (47.13%), Query Frame = 0

Query: 49  QDTAAATVEELTTFEGGEDLTIDEILEAPGE--VIGKYHYGTLYKASLQSTQRTT---LL 108
           ++ + A    LT F+   D T D++L++  E  +IGK   G +YK ++          L 
Sbjct: 663 RNASEAKAWRLTAFQ-RLDFTCDDVLDSLKEDNIIGKGGAGIVYKGTMPKGDLVAVKRLA 722

Query: 109 MFLRPAAVESSVDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVG 168
                ++ +   +  ++ LG IRHR++V +LGF        LLV+ +   G+L E +   
Sbjct: 723 TMSHGSSHDHGFNAEIQTLGRIRHRHIVRLLGF-CSNHETNLLVYEYMPNGSLGEVLHGK 782

Query: 169 ESESKNWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLL 228
           +    +W   YKI+L  AK L +LHH     + H ++K  NI LD +FE +V+DFGL   
Sbjct: 783 KGGHLHWNTRYKIALEAAKGLCYLHHDCSPLIVHRDVKSNNILLDSNFEAHVADFGLAKF 842

Query: 229 LNAAATRELLQASSAS-GYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVAC 288
           L  + T E + A + S GY APE       DE++DVYS+G +LLEL++ K       V  
Sbjct: 843 LQDSGTSECMSAIAGSYGYIAPEYAYTLKVDEKSDVYSFGVVLLELITGK-----KPVGE 902

Query: 289 GGDGGD----CSVSKERNLQC-------------------LFQIGEACCCSSPIRRPDFK 334
            GDG D         + N  C                   +F +   C     + RP  +
Sbjct: 903 FGDGVDIVQWVRSMTDSNKDCVLKVIDLRLSSVPVHEVTHVFYVALLCVEEQAVERPTMR 962

BLAST of Carg08499 vs. ExPASy TrEMBL
Match: A0A6J1H0H8 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 331/334 (99.10%), Postives = 332/334 (99.40%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLLVSIFF RHKLSKTNHR+DIENPSPDNNQKKQDTAAATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFLRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACC SSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499 vs. ExPASy TrEMBL
Match: A0A6J1K4E4 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.3e-182
Identity = 324/334 (97.01%), Postives = 330/334 (98.80%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLILIVASATIFVLLL+SIFFFRHKLSK+NHRHDIENPSPDNNQKKQDT AATVEELT
Sbjct: 1   MLKLILIVASATIFVLLLLSIFFFRHKLSKSNHRHDIENPSPDNNQKKQDTTAATVEELT 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDVVR 120
           TF+GGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQR TLLMFLRPAAVESSVDDVVR
Sbjct: 61  TFQGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRATLLMFLRPAAVESSVDDVVR 120

Query: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180
           FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI
Sbjct: 121 FLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISLGI 180

Query: 181 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 240
           AKALDHLHHGLPKPVPHGNLKP NIFLDP+FEPYVSDFGLHLLLNAAATRELLQASSASG
Sbjct: 181 AKALDHLHHGLPKPVPHGNLKPMNIFLDPNFEPYVSDFGLHLLLNAAATRELLQASSASG 240

Query: 241 YNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQCL 300
           YNAPELV+MTDADERTDVYSYGKILLELLSAK+TMN TAVACGGDGGDCSVSKERNLQCL
Sbjct: 241 YNAPELVKMTDADERTDVYSYGKILLELLSAKETMNQTAVACGGDGGDCSVSKERNLQCL 300

Query: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD
Sbjct: 301 FQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

BLAST of Carg08499 vs. ExPASy TrEMBL
Match: A0A6J1I3Q5 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.1e-134
Identity = 253/337 (75.07%), Postives = 279/337 (82.79%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSI---FFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVE 60
           MLK IL+VASA IFVLLL+SI   FFFRHKLS T HRHD+E P  D N+KK+ T   +  
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCTDQNKKKEHTPPPSPP 60

Query: 61  ELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDD 120
            L TFEGGEDLTIDEILEAPGEVIGK HYGTLYKASLQSTQ T LL FLRP A      D
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEY-D 120

Query: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKIS 180
           VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG+GNL++ I VGESE+KNWE+I KIS
Sbjct: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLADLIRVGESETKNWEVINKIS 180

Query: 181 LGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASS 240
           LGIA+ALDHLH+GL KP+PHGNLK KNI LD +FEP+VSDFGLHLLLNAAATRE+LQ S+
Sbjct: 181 LGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQVSA 240

Query: 241 ASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNL 300
           ASGY APEL+ M DADERTD++SYGKILLELLSAK+T        GGDGGDC VS+ER L
Sbjct: 241 ASGYKAPELINMNDADERTDIFSYGKILLELLSAKET--------GGDGGDCGVSEERTL 300

Query: 301 QCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           +C+ QIGEAC  SSP  RP+FKEVI KI EIGRIEMD
Sbjct: 301 RCILQIGEACSSSSPDLRPNFKEVIAKIAEIGRIEMD 328

BLAST of Carg08499 vs. ExPASy TrEMBL
Match: A0A6J1ELS2 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.8e-134
Identity = 254/336 (75.60%), Postives = 278/336 (82.74%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSI--FFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEE 60
           MLK +L+VASA IFVLLL+SI  FFFRHKLS T HRHD+E P  D + KK+ T   +   
Sbjct: 1   MLKFMLLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60

Query: 61  LTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESSVDDV 120
           L TFEGGEDLTIDEILEAPGEVIGK HYGTLYKASLQSTQ T LL FLRP A      DV
Sbjct: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQATRLLRFLRPGAGGGEY-DV 120

Query: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKISL 180
           VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG+GNL+E I   ESESKNWE+I KISL
Sbjct: 121 VRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIRERESESKNWEVINKISL 180

Query: 181 GIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSA 240
           GIA+ALDHLH+GL KP+PHGNLK KNI LD +FEP+VSDFGLHLLLNAAATRE+L+ S+A
Sbjct: 181 GIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKVSAA 240

Query: 241 SGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGDGGDCSVSKERNLQ 300
           SGY APEL+ MTDADERTDV+SYGKILLELLSA++T        GGDGGDC  S+ER L+
Sbjct: 241 SGYKAPELINMTDADERTDVFSYGKILLELLSAEET--------GGDGGDCGGSEERTLR 300

Query: 301 CLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           CL QIGEACC SSP RRPDFKEVI KI EIGRIEMD
Sbjct: 301 CLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIEMD 327

BLAST of Carg08499 vs. ExPASy TrEMBL
Match: A0A6J1DVQ7 (putative kinase-like protein TMKL1 OS=Momordica charantia OX=3673 GN=LOC111023538 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 1.2e-114
Identity = 231/338 (68.34%), Postives = 265/338 (78.40%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           MLKLIL+VA+A IF++ ++ +F FRHKL+KT  R D+E        K     A   EEL+
Sbjct: 1   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQR-DVE--------KAAAAKAEEEEELS 60

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRP-AAVESSVDDVV 120
           TFEGGEDLT+DEILEAPGEVIGK  YGTLYKASLQ +QRT +L FLRP AA E S DD V
Sbjct: 61  TFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTV 120

Query: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVG--ESESKNWEIIYKIS 180
            FLGSIRH NLVPMLGFYAG RGE+LLVHPFYGRGNL++FITVG  E+ES+ WEIIYKIS
Sbjct: 121 GFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKIS 180

Query: 181 LGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASS 240
           +GIAKALDHLH  L KP+ HGNLK KNI LD +FEP+VSDFGLHLLLN AA +ELL+ S+
Sbjct: 181 MGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSA 240

Query: 241 ASGYNAPELVRMTDADERTDVYSYGKILLE-LLSAKDTMNLTAVACGGDGGDCSVSKERN 300
            SGY APEL++MTDADERTDVYSYGKILLE LL  KD  +       GDGGDC VS +R 
Sbjct: 241 DSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRI 300

Query: 301 LQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 335
           L+C  +I EACC SSP  RP+FKEVI KIEEIGRI++D
Sbjct: 301 LRC-SRIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 328

BLAST of Carg08499 vs. TAIR 10
Match: AT5G07620.1 (Protein kinase superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 3.5e-82
Identity = 168/360 (46.67%), Postives = 229/360 (63.61%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60
           +L  +L+  S + F++++  I F R K S +      +  S D+N  KQ  ++ T EEL 
Sbjct: 5   LLLTLLLGVSLSAFLIVIFFIIFLRRKESSSTESDQYDVESLDHN--KQGFSSET-EELV 64

Query: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESS----VD 120
            F+GGEDLTI +IL+APGEVIGK  YGTLYKASLQ + +  +L FLRP     S     +
Sbjct: 65  IFQGGEDLTICDILDAPGEVIGKSSYGTLYKASLQRSGKIRVLRFLRPVCTVRSDSKEFN 124

Query: 121 DVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESESKNWEIIYKI 180
            ++  LG +RH NLVP+LGFYAG RGE+L+VHPF+G GNLS+FI  G+ ES+ W  I +I
Sbjct: 125 GIIETLGFVRHENLVPLLGFYAGNRGEKLMVHPFFGSGNLSDFIRSGDDESRKWINILRI 184

Query: 181 SLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQAS 240
           ++GI+KALDHLH G+ KP+ HGNLK KN+ L   FEP +SDFGLHLLLN +A +E+L  S
Sbjct: 185 TIGISKALDHLHTGMQKPIVHGNLKSKNVLLSSSFEPRISDFGLHLLLNLSAGQEILDVS 244

Query: 241 SASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVACGGD----------- 300
           +A GY APEL++M D  + +DVYS G I+LEL+S K+ +N  A   G D           
Sbjct: 245 AAEGYKAPELIKMKDVSKESDVYSLGVIMLELVSGKEPINENAT--GDDEFYLPDFMRNA 304

Query: 301 ---------------GGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGR 331
                          G D ++S+E  L+  FQ+  +CC  SP  RP+ K+V+ K+EEIG+
Sbjct: 305 VLDHRLSDLYRPEILGSDDNLSEECVLK-YFQLAMSCCSPSPSLRPNVKQVLRKLEEIGK 358

BLAST of Carg08499 vs. TAIR 10
Match: AT5G61570.2 (Protein kinase superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.3e-81
Identity = 169/356 (47.47%), Postives = 227/356 (63.76%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFF---RHKLSKTNH-RHDIENPSPDNNQKKQDTAAATV 60
           ++KLIL V+  T F++++  I  F   R   ++TN  ++D+E  SP   Q+  D  + T 
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVE--SPYEKQEFSDNGSETE 65

Query: 61  EELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESS-- 120
           EEL  F GGEDLTI +IL+APGEVIGK  YGTLYKA+LQ + +  +L FLRP    +S  
Sbjct: 66  EELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNSDS 125

Query: 121 --VDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG-RGNLSEFITVGESESKNWE 180
              + V+  LG +RH NLVP+LGFY G RGE+L++HPF+G  GNLS FI  G+ ++  W 
Sbjct: 126 KEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKCGDVDAHKWS 185

Query: 181 IIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRE 240
            I  I++GIAKALDHLH G+ KP+ HGNLK KN+ LD  F P VSDFGLHLLLN AA +E
Sbjct: 186 NILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNLAAGQE 245

Query: 241 LLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVA---------- 300
           +L+AS+A GY APEL++M +  + +DVYS+G I+LEL+S K+  N               
Sbjct: 246 VLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLELVSGKEPTNKNPTGSVLDRNRLSD 305

Query: 301 ---------CGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
                    C  DG    V++E  L+  FQ+  +CC  SP  RP FK+V+ K+EEI
Sbjct: 306 LYRPEIIRRCLKDGN--GVTEECVLE-YFQLAMSCCSPSPTLRPSFKQVLRKLEEI 356

BLAST of Carg08499 vs. TAIR 10
Match: AT5G61570.1 (Protein kinase superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.6e-80
Identity = 169/359 (47.08%), Postives = 227/359 (63.23%), Query Frame = 0

Query: 1   MLKLILIVASATIFVLLLVSIFFF---RHKLSKTNH-RHDIENPSPDNNQKKQDTAAATV 60
           ++KLIL V+  T F++++  I  F   R   ++TN  ++D+E  SP   Q+  D  + T 
Sbjct: 6   LVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVE--SPYEKQEFSDNGSETE 65

Query: 61  EELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVESS-- 120
           EEL  F GGEDLTI +IL+APGEVIGK  YGTLYKA+LQ + +  +L FLRP    +S  
Sbjct: 66  EELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNSDS 125

Query: 121 --VDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYG-RGNLSEFITV---GESESK 180
              + V+  LG +RH NLVP+LGFY G RGE+L++HPF+G  GNLS FI     G+ ++ 
Sbjct: 126 KEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKFLAGGDVDAH 185

Query: 181 NWEIIYKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAA 240
            W  I  I++GIAKALDHLH G+ KP+ HGNLK KN+ LD  F P VSDFGLHLLLN AA
Sbjct: 186 KWSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNLAA 245

Query: 241 TRELLQASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKDTMNLTAVA------- 300
            +E+L+AS+A GY APEL++M +  + +DVYS+G I+LEL+S K+  N            
Sbjct: 246 GQEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLELVSGKEPTNKNPTGSVLDRNR 305

Query: 301 ------------CGGDGGDCSVSKERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
                       C  DG    V++E  L+  FQ+  +CC  SP  RP FK+V+ K+EEI
Sbjct: 306 LSDLYRPEIIRRCLKDGN--GVTEECVLE-YFQLAMSCCSPSPTLRPSFKQVLRKLEEI 359

BLAST of Carg08499 vs. TAIR 10
Match: AT3G24660.1 (transmembrane kinase-like 1 )

HSP 1 Score: 164.1 bits (414), Expect = 2.0e-40
Identity = 107/350 (30.57%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 4   LILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELTTFE 63
           +I +++ A +   LL+     + + S      D+E    ++   +++       +L  F+
Sbjct: 305 VIGLMSGAVVVASLLIGYLQNKKRKSSIESEDDLEEGDEEDEIGEKEGGEG---KLVVFQ 364

Query: 64  GGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE-SSVDDVVRFL 123
           GGE+LT+D++L A G+V+ K  YGT+YKA L       L +       + SS   V+R L
Sbjct: 365 GGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNIALRLLREGTCKDRSSCLPVIRQL 424

Query: 124 GSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFI--TVGESESKNWEIIYKISLGI 183
           G IRH NLVP+  FY G RGE+LL++ +    +L + +  +     + NW   +KI+LGI
Sbjct: 425 GRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIALGI 484

Query: 184 AKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASSASG 243
           A+ L +LH G   P+ HGN++ KN+ +D  F   +++FGL  ++  A   E++  + + G
Sbjct: 485 ARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKSDG 544

Query: 244 YNAPELVRMTDADERTDVYSYGKILLELLSAK-------------DTMNLTAVACGGDGG 303
           Y APEL +M   + R+DVY++G +LLE+L  K             D  +L   A   +  
Sbjct: 545 YKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGKSGRNGNEFVDLPSLVKAAVLEETT 604

Query: 304 ----DCSVSK------ERNLQCLFQIGEACCCSSPIRRPDFKEVIEKIEE 328
               D    K      E  L    ++   CC      RP  +EV++++EE
Sbjct: 605 MEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEE 651

BLAST of Carg08499 vs. TAIR 10
Match: AT3G51740.1 (inflorescence meristem receptor-like kinase 2 )

HSP 1 Score: 138.7 bits (348), Expect = 8.8e-33
Identity = 104/353 (29.46%), Postives = 164/353 (46.46%), Query Frame = 0

Query: 4   LILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVE------ 63
           +ILI   A + +LLL+        + K       +     + +      A T        
Sbjct: 459 VILIAIGALLAILLLLCCILLCCLIKKRAALKQKDGKDKTSEKTVSAGVAGTASAGGEMG 518

Query: 64  -ELTTFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAVE--SS 123
            +L  F+G    T D++L A  E++GK  YGT YKA+L+      +   LR    +    
Sbjct: 519 GKLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKR-LREKTTKGVKE 578

Query: 124 VDDVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITV-GESESKNWEII 183
            +  V  LG IRH+NL+ +  +Y G +GE+LLV  +  +G+LS F+   G      WE  
Sbjct: 579 FEGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETR 638

Query: 184 YKISLGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELL 243
            KI+ GI++ L HLH    + + H NL   NI LD     +++D+GL  L+ AAA   ++
Sbjct: 639 MKIAKGISRGLAHLHSN--ENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVI 698

Query: 244 QASSASGYNAPELVRMTDADERTDVYSYGKILLELLSAKD-------------TMNLTAV 303
             +   GY APE  ++ +A  +TDVYS G I+LELL+ K                ++   
Sbjct: 699 ATAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGEPTNGMDLPQWVASIVKE 758

Query: 304 ACGGDGGDCSVSKER-----NLQCLFQIGEACCCSSPIRRPDFKEVIEKIEEI 329
               +  D  + +E       L    ++   C   SP  RP+  +V+E++EEI
Sbjct: 759 EWTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 808

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6605638.14.9e-187100.00putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022957896.12.3e-18499.10putative kinase-like protein TMKL1 [Cucurbita moschata][more]
XP_023532398.15.7e-18398.50putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo][more]
XP_022994989.14.8e-18297.01putative kinase-like protein TMKL1 [Cucurbita maxima][more]
XP_023520536.15.7e-13576.19putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 ... [more]
Match NameE-valueIdentityDescription
P335432.8e-3930.57Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1... [more]
C0LGP99.5e-3232.03Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis th... [more]
Q9SCT41.2e-3129.46Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabi... [more]
Q9S7I62.3e-3029.71LRR receptor-like serine/threonine-protein kinase RPK2 OS=Arabidopsis thaliana O... [more]
Q9M2Z17.5e-2929.94Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM2 OS=Arabid... [more]
Match NameE-valueIdentityDescription
A0A6J1H0H81.1e-18499.10putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294... [more]
A0A6J1K4E42.3e-18297.01putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 P... [more]
A0A6J1I3Q51.1e-13475.07putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 P... [more]
A0A6J1ELS21.8e-13475.60putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777... [more]
A0A6J1DVQ71.2e-11468.34putative kinase-like protein TMKL1 OS=Momordica charantia OX=3673 GN=LOC11102353... [more]
Match NameE-valueIdentityDescription
AT5G07620.13.5e-8246.67Protein kinase superfamily protein [more]
AT5G61570.21.3e-8147.47Protein kinase superfamily protein [more]
AT5G61570.15.6e-8047.08Protein kinase superfamily protein [more]
AT3G24660.12.0e-4030.57transmembrane kinase-like 1 [more]
AT3G51740.18.8e-3329.46inflorescence meristem receptor-like kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 69..334
e-value: 4.8E-45
score: 155.8
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 79..329
e-value: 9.4E-8
score: 28.9
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 79..329
e-value: 9.4E-8
score: 28.9
NoneNo IPR availablePANTHERPTHR48008:SF13PROTEIN KINASE SUPERFAMILY PROTEINcoord: 2..276
NoneNo IPR availablePANTHERPTHR48008:SF13PROTEIN KINASE SUPERFAMILY PROTEINcoord: 291..329
NoneNo IPR availablePANTHERPTHR48008LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATEDcoord: 2..276
NoneNo IPR availablePANTHERPTHR48008LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATEDcoord: 291..329
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 79..324
e-value: 1.7E-22
score: 80.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 75..328
score: 22.605225
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 78..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08499-RACarg08499-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity