Carg08474 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08474
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncell division cycle 20.2, cofactor of APC complex-like
LocationCarg_Chr02: 5489395 .. 5492045 (-)
RNA-Seq ExpressionCarg08474
SyntenyCarg08474
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAATCTTCAAACGCTCTCTATTCATCGCTCACTCTCACTGCGCTCTTCAAACGCTCTCAACAATCAATCGAAACTTAATTGTTTCTTTTTTTCTGATATTTGCTTCTTGATTTCTGCTTTCGATATTCTGTTTTTGTGGATCTTACGAATTTCTTTGCAGGGTTTCAAATTGTTTATCTTTCTCTGTTTTTTCTGTTGGATATTGTGTTCTTGTGTTATTCTTTTTTGGAAATTTACGCTATGGATGCAGGATCTTTGAGTTCTTCTAATGCTGGTCGATCGCGCTGTCCCCTGCAAGAACAGCATCTTCAGAAAAAGCATTCCCGAGAAAATGTAAGTGTTTGTTCGTAATTGTAGTGATTGAAATCAATTAGAGGAAGATGAAGGATTTGTTTATGTGAAATTGTTCTGAGTCGATGAATTAGGTTTTGATTTCTGTCTCTTTTCAATTACAGTTGGATAGATTTATTCCGAATCGATCGGCGATGGATTTCGATTACGCGCATTACATGGTGACTGATGGGAGGAAAGGTAAGGAAAATCCTTCAATTTGTTCGCCCTCGAGGGAGGCTTATCAGAAGCGTTTGGCTGAGACGTTGAACATGAATCGAACTCGGATCCTTGCCTTCAGAAACAAACCTCCTGCGCCGGTTGAGTTGATCCCCAAGGAGTTCTTCTCCTCGGGTTCTAACGATAAACCTGTCAAGGCCCGCCGCCATATCCCCCAGGTGAGTTGCAGTTCAAGTGTTTGATAATATGCCTCAATGAATGGGTGAATTTGTTTGAAGAAGAAAAGTCATTAATTGGGTTTATTTTTCTGTTGTAGACCTCTGAAAGGACGTTGGATGCTCCTGATCTTGTTGATGATTATTACTTGAACTTGTTGGATTGGGGCAGCGCCAATGTGCTTGCTATTGCTCTTGGCAACACTGTGTATTTGTGGAATGCAAACGATGGCTCTACTTCAGAACTTGTCACTGTTGATGATGAAGTTGGTCCTATCACTAGTGTCAACTGGGCACCTGATGGACGACACATCGGTATTGGGTTTCATAACTCTGAAGTGCAGCTCTGGGATTCAACCGCTAACCGACAGGTAATCTCTCGCATTCTGCTAAGGAAGAAATTAGAAAAATAACGAATTCTATAGGTTGATGCCACAAATTCCACCTCAAAAACCATATTTCGCTTGAGCCTCAACCATATCAACTGTACTCGAATGGTTAGATAATCATAGTTGATAATAACATCACCGTTCTCATCGCTATTACAGAACCATACATGAGATTTTCACCTCATACAGCTCCTTGAGGGCCATAAATTAATCTAAAAGCTTATCGGGGGATTATTTTTCTTAATATGTCTTTGTTATTTTTTTTGCCCTTACATGACTAGAATTTCTTCTGGGTGATGAATTTTTACAGCTAAGAACTCTAAGAGGTGGGCATAGGATGCGAGTGGGTTCACTGGCATGGAACAACCATATTCTCACCACAGGAGGAATGGATGGAAAGATCATAAACAATGATGTGAGAATAAGAGATCATATAGTCGAAACTTACAGAGGTCATGATCAAGAGGTTTGTGGACTCAAATGGTCATTATCAGGGCAACAATTAGCAAGCGGAGGAAATGACAATGTCCTCCACATTTGGGACCGATCAATGGCCTCTTCAAACTCAGCAACCCAGTGGCTTCACAGGCTTGAGGATCATACATCTGCAGTCAAGGCTCTTGCATGGTGTCCATTCCAAAGCAACTTGCTCGCAACCGGTGGAGGTGCTAGTGACCGCACCATAAAGTTTTGGAACACGCACACTGGCGCATGTTTGAATTCTGTTGATACTGGTTCTCAAGTCTGTGCTCTGCTGTGGAATAAAAATGAGAGAGAGCTGCTTAGCTCTCATGGGTTCTCCCAGAATCAGCTCACTCTTTGGAAGTACCCTTCAATGGTGAAGATGGGAGAGCTCACCGGCCATACATCAAGAGTTCTCTTTATGGCTCAGGTACGGTACATGTACATGTATTTTCTGATGAATATGATGATGATGGCTTGGTTTGTTCATTTTGTTTGTTGATGGGTTTTTTCTGATTGTTCTTTGTGGTGTGATGATTCTACTACAGAGTCCAGATGGGTGCACTGTGGCTTCAGCAGCAGCTGATGAAACATTGAGATTCTGGAATGTTTTTGGGGCTCCAGAAGTGGCTAAACCTGCTGCAAAATCGTACAATGCAGAGCCCTTTGCTCATATCAATCGCATTCGATAAGCTACGTGTGGTGATACTTTCACTTTCCTGTAATACACATGATATCTACAGCAATACACATGATATCTACAGCAATTTTGTGGTATAATATACAAGTAGGAGGACTCAAAATTAGGTTGATACAGAATAATTGTTACAACACCACCTCGTTGTACACAGATATACATGATGTTCATTAGAGAGATGTACGGGTCATTTTTTAATTAAAGAAAATGTTCTTGTTTTTGTCCTTTTCCAATGAAGTACTTACAAAATTATGTGATTACTATTTAAATCTAGTGTGGTGTGTAATGTGGGGAGACTACAAAATAAGATAAATGTCTCTCAGAATCGAGAGGTATGAGATTGATGTAATAGTCAAGAGGTATGAGATTGAAGTAAG

mRNA sequence

TCAATCTTCAAACGCTCTCTATTCATCGCTCACTCTCACTGCGCTCTTCAAACGCTCTCAACAATCAATCGAAACTTAATTGTTTCTTTTTTTCTGATATTTGCTTCTTGATTTCTGCTTTCGATATTCTGTTTTTGTGGATCTTACGAATTTCTTTGCAGGGTTTCAAATTGTTTATCTTTCTCTGTTTTTTCTGTTGGATATTGTGTTCTTGTGTTATTCTTTTTTGGAAATTTACGCTATGGATGCAGGATCTTTGAGTTCTTCTAATGCTGGTCGATCGCGCTGTCCCCTGCAAGAACAGCATCTTCAGAAAAAGCATTCCCGAGAAAATTTGGATAGATTTATTCCGAATCGATCGGCGATGGATTTCGATTACGCGCATTACATGGTGACTGATGGGAGGAAAGGTAAGGAAAATCCTTCAATTTGTTCGCCCTCGAGGGAGGCTTATCAGAAGCGTTTGGCTGAGACGTTGAACATGAATCGAACTCGGATCCTTGCCTTCAGAAACAAACCTCCTGCGCCGGTTGAGTTGATCCCCAAGGAGTTCTTCTCCTCGGGTTCTAACGATAAACCTGTCAAGGCCCGCCGCCATATCCCCCAGACCTCTGAAAGGACGTTGGATGCTCCTGATCTTGTTGATGATTATTACTTGAACTTGTTGGATTGGGGCAGCGCCAATGTGCTTGCTATTGCTCTTGGCAACACTGTGTATTTGTGGAATGCAAACGATGGCTCTACTTCAGAACTTGTCACTGTTGATGATGAAGTTGGTCCTATCACTAGTGTCAACTGGGCACCTGATGGACGACACATCGGTATTGGGTTTCATAACTCTGAAGTGCAGCTCTGGGATTCAACCGCTAACCGACAGCTAAGAACTCTAAGAGGTGGGCATAGGATGCGAGTGGGTTCACTGGCATGGAACAACCATATTCTCACCACAGGAGGAATGGATGGAAAGATCATAAACAATGATGTGAGAATAAGAGATCATATAGTCGAAACTTACAGAGGTCATGATCAAGAGGTTTGTGGACTCAAATGGTCATTATCAGGGCAACAATTAGCAAGCGGAGGAAATGACAATGTCCTCCACATTTGGGACCGATCAATGGCCTCTTCAAACTCAGCAACCCAGTGGCTTCACAGGCTTGAGGATCATACATCTGCAGTCAAGGCTCTTGCATGGTGTCCATTCCAAAGCAACTTGCTCGCAACCGGTGGAGGTGCTAGTGACCGCACCATAAAGTTTTGGAACACGCACACTGGCGCATGTTTGAATTCTGTTGATACTGGTTCTCAAGTCTGTGCTCTGCTGTGGAATAAAAATGAGAGAGAGCTGCTTAGCTCTCATGGGTTCTCCCAGAATCAGCTCACTCTTTGGAAGTACCCTTCAATGGTGAAGATGGGAGAGCTCACCGGCCATACATCAAGAGTTCTCTTTATGGCTCAGAGTCCAGATGGGTGCACTGTGGCTTCAGCAGCAGCTGATGAAACATTGAGATTCTGGAATGTTTTTGGGGCTCCAGAAGTGGCTAAACCTGCTGCAAAATCGTACAATGCAGAGCCCTTTGCTCATATCAATCGCATTCGATAAGCTACGTGTGGTGATACTTTCACTTTCCTGTAATACACATGATATCTACAGCAATACACATGATATCTACAGCAATTTTGTGGTATAATATACAAGTAGGAGGACTCAAAATTAGGTTGATACAGAATAATTGTTACAACACCACCTCGTTGTACACAGATATACATGATGTTCATTAGAGAGATGTACGGGTCATTTTTTAATTAAAGAAAATGTTCTTGTTTTTGTCCTTTTCCAATGAAGTACTTACAAAATTATGTGATTACTATTTAAATCTAGTGTGGTGTGTAATGTGGGGAGACTACAAAATAAGATAAATGTCTCTCAGAATCGAGAGGTATGAGATTGATGTAATAGTCAAGAGGTATGAGATTGAAGTAAG

Coding sequence (CDS)

ATGGATGCAGGATCTTTGAGTTCTTCTAATGCTGGTCGATCGCGCTGTCCCCTGCAAGAACAGCATCTTCAGAAAAAGCATTCCCGAGAAAATTTGGATAGATTTATTCCGAATCGATCGGCGATGGATTTCGATTACGCGCATTACATGGTGACTGATGGGAGGAAAGGTAAGGAAAATCCTTCAATTTGTTCGCCCTCGAGGGAGGCTTATCAGAAGCGTTTGGCTGAGACGTTGAACATGAATCGAACTCGGATCCTTGCCTTCAGAAACAAACCTCCTGCGCCGGTTGAGTTGATCCCCAAGGAGTTCTTCTCCTCGGGTTCTAACGATAAACCTGTCAAGGCCCGCCGCCATATCCCCCAGACCTCTGAAAGGACGTTGGATGCTCCTGATCTTGTTGATGATTATTACTTGAACTTGTTGGATTGGGGCAGCGCCAATGTGCTTGCTATTGCTCTTGGCAACACTGTGTATTTGTGGAATGCAAACGATGGCTCTACTTCAGAACTTGTCACTGTTGATGATGAAGTTGGTCCTATCACTAGTGTCAACTGGGCACCTGATGGACGACACATCGGTATTGGGTTTCATAACTCTGAAGTGCAGCTCTGGGATTCAACCGCTAACCGACAGCTAAGAACTCTAAGAGGTGGGCATAGGATGCGAGTGGGTTCACTGGCATGGAACAACCATATTCTCACCACAGGAGGAATGGATGGAAAGATCATAAACAATGATGTGAGAATAAGAGATCATATAGTCGAAACTTACAGAGGTCATGATCAAGAGGTTTGTGGACTCAAATGGTCATTATCAGGGCAACAATTAGCAAGCGGAGGAAATGACAATGTCCTCCACATTTGGGACCGATCAATGGCCTCTTCAAACTCAGCAACCCAGTGGCTTCACAGGCTTGAGGATCATACATCTGCAGTCAAGGCTCTTGCATGGTGTCCATTCCAAAGCAACTTGCTCGCAACCGGTGGAGGTGCTAGTGACCGCACCATAAAGTTTTGGAACACGCACACTGGCGCATGTTTGAATTCTGTTGATACTGGTTCTCAAGTCTGTGCTCTGCTGTGGAATAAAAATGAGAGAGAGCTGCTTAGCTCTCATGGGTTCTCCCAGAATCAGCTCACTCTTTGGAAGTACCCTTCAATGGTGAAGATGGGAGAGCTCACCGGCCATACATCAAGAGTTCTCTTTATGGCTCAGAGTCCAGATGGGTGCACTGTGGCTTCAGCAGCAGCTGATGAAACATTGAGATTCTGGAATGTTTTTGGGGCTCCAGAAGTGGCTAAACCTGCTGCAAAATCGTACAATGCAGAGCCCTTTGCTCATATCAATCGCATTCGATAA

Protein sequence

MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKENPSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHIPQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSATQWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Homology
BLAST of Carg08474 vs. NCBI nr
Match: KAG7035573.1 (Cell division cycle 20.1, cofactor of APC complex [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 939.9 bits (2428), Expect = 8.3e-270
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN
Sbjct: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI
Sbjct: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Sbjct: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 453

BLAST of Carg08474 vs. NCBI nr
Match: XP_023532453.1 (cell division cycle 20.2, cofactor of APC complex-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 934.9 bits (2415), Expect = 2.7e-268
Identity = 451/453 (99.56%), Postives = 451/453 (99.56%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN
Sbjct: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI
Sbjct: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKIINNDVRIRDHIV TYRGHDQEVCGLKWS SGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKIINNDVRIRDHIVGTYRGHDQEVCGLKWSSSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Sbjct: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 453

BLAST of Carg08474 vs. NCBI nr
Match: XP_022995242.1 (cell division cycle 20.2, cofactor of APC complex-like [Cucurbita maxima])

HSP 1 Score: 929.5 bits (2401), Expect = 1.1e-266
Identity = 449/453 (99.12%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDA SLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN
Sbjct: 1   MDAASLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
            SICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI
Sbjct: 61  SSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           ITSVNWAPDGRHI IGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 ITSVNWAPDGRHIAIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKIINNDVRIRDHIV TYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKIINNDVRIRDHIVGTYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Sbjct: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 453

BLAST of Carg08474 vs. NCBI nr
Match: XP_022958728.1 (cell division cycle 20.2, cofactor of APC complex-like [Cucurbita moschata])

HSP 1 Score: 928.7 bits (2399), Expect = 1.9e-266
Identity = 449/453 (99.12%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLSSSNAGRSRCPLQEQ LQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN
Sbjct: 1   MDAGSLSSSNAGRSRCPLQEQQLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI
Sbjct: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           ITSVNWAPDGRHIGIGF NSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 ITSVNWAPDGRHIGIGFQNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKIINNDVRIRDHIV TYRGHDQEVCGLKWS SGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKIINNDVRIRDHIVGTYRGHDQEVCGLKWSSSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Sbjct: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 453

BLAST of Carg08474 vs. NCBI nr
Match: KAG6605663.1 (Cell division cycle 20.1, cofactor of APC complex, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 438/453 (96.69%), Postives = 438/453 (96.69%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN
Sbjct: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI
Sbjct: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           ITSVNWAPDGRHI       E  L D     QLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 ITSVNWAPDGRHI-------EFLLGDEFL--QLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Sbjct: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 444

BLAST of Carg08474 vs. ExPASy Swiss-Prot
Match: Q9SZA4 (Cell division cycle 20.1, cofactor of APC complex OS=Arabidopsis thaliana OX=3702 GN=CDC20-1 PE=1 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 7.9e-215
Identity = 358/457 (78.34%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MDAG--SLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGK 60
           MDAG  + SS    ++RCPLQE  L +K S+ENLDRFIPNRSAM+FDYAH+ +T+GRKGK
Sbjct: 1   MDAGMNNTSSHYKTQARCPLQEHFLPRKPSKENLDRFIPNRSAMNFDYAHFALTEGRKGK 60

Query: 61  -ENPSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSS-GSNDKPVKA 120
            +  ++ SPS+EAY+K+LAET+N+N TRILAFRNKP APVEL+P    +S     K VK 
Sbjct: 61  DQTAAVSSPSKEAYRKQLAETMNLNHTRILAFRNKPQAPVELLPSNHSASLHQQPKSVKP 120

Query: 121 RRHIPQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDD 180
           RR+IPQTSERTLDAPD+VDD+YLNLLDWGSANVLAIAL +TVYLW+A+ GSTSELVT+D+
Sbjct: 121 RRYIPQTSERTLDAPDIVDDFYLNLLDWGSANVLAIALDHTVYLWDASTGSTSELVTIDE 180

Query: 181 EVGPITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTT 240
           E GP+TS+NWAPDGRH+ +G +NSEVQLWDS +NRQLRTL+GGH+ RVGSLAWNNHILTT
Sbjct: 181 EKGPVTSINWAPDGRHVAVGLNNSEVQLWDSASNRQLRTLKGGHQSRVGSLAWNNHILTT 240

Query: 241 GGMDGKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASS 300
           GGMDG IINNDVRIR  IVETYRGH QEVCGLKWS SGQQLASGGNDNV+HIWDRS+ASS
Sbjct: 241 GGMDGLIINNDVRIRSPIVETYRGHTQEVCGLKWSGSGQQLASGGNDNVVHIWDRSVASS 300

Query: 301 NSATQWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQ 360
           NS TQWLHRLE+HTSAVKALAWCPFQ+NLLATGGG  DRTIKFWNTHTGACLNSVDTGSQ
Sbjct: 301 NSTTQWLHRLEEHTSAVKALAWCPFQANLLATGGGGGDRTIKFWNTHTGACLNSVDTGSQ 360

Query: 361 VCALLWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASA 420
           VC+LLW+KNERELLSSHGF+QNQLTLWKYPSMVKM ELTGHTSRVL+MAQSPDGCTVASA
Sbjct: 361 VCSLLWSKNERELLSSHGFTQNQLTLWKYPSMVKMAELTGHTSRVLYMAQSPDGCTVASA 420

Query: 421 AADETLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           A DETLRFWNVFG PE AK AA    +EPF+H+NRIR
Sbjct: 421 AGDETLRFWNVFGVPETAKKAAPKAVSEPFSHVNRIR 457

BLAST of Carg08474 vs. ExPASy Swiss-Prot
Match: Q9S7I8 (Cell division cycle 20.2, cofactor of APC complex OS=Arabidopsis thaliana OX=3702 GN=CDC20-2 PE=1 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 1.3e-214
Identity = 354/446 (79.37%), Postives = 400/446 (89.69%), Query Frame = 0

Query: 10  NAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGK-ENPSICSPSR 69
           +AG +RCPLQE  L +K+S+ENLDRFIPNRSAM+FDYAH+ +T+ RKGK ++ ++ SPS+
Sbjct: 2   DAGLNRCPLQEHFLPRKNSKENLDRFIPNRSAMNFDYAHFALTEERKGKDQSATVSSPSK 61

Query: 70  EAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSS-GSNDKPVKARRHIPQTSERT 129
           EAY+K+LAET+N+N TRILAFRNKP APVEL+P    +S     K VK RR+IPQTSERT
Sbjct: 62  EAYRKQLAETMNLNHTRILAFRNKPQAPVELLPSNHSASLHQQPKSVKPRRYIPQTSERT 121

Query: 130 LDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWA 189
           LDAPD+VDD+YLNLLDWGSANVLAIAL +TVYLW+A+ GSTSELVT+D+E GP+TS+NWA
Sbjct: 122 LDAPDIVDDFYLNLLDWGSANVLAIALDHTVYLWDASTGSTSELVTIDEEKGPVTSINWA 181

Query: 190 PDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINND 249
           PDGRH+ +G +NSEVQLWDS +NRQLRTL+GGH+ RVGSLAWNNHILTTGGMDG IINND
Sbjct: 182 PDGRHVAVGLNNSEVQLWDSASNRQLRTLKGGHQSRVGSLAWNNHILTTGGMDGLIINND 241

Query: 250 VRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSATQWLHRLE 309
           VRIR  IVETYRGH QEVCGLKWS SGQQLASGGNDNV+HIWDRS+ASSNS TQWLHRLE
Sbjct: 242 VRIRSPIVETYRGHTQEVCGLKWSGSGQQLASGGNDNVVHIWDRSVASSNSTTQWLHRLE 301

Query: 310 DHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNER 369
           +HTSAVKALAWCPFQ+NLLATGGG  DRTIKFWNTHTGACLNSVDTGSQVC+LLW+KNER
Sbjct: 302 EHTSAVKALAWCPFQANLLATGGGGGDRTIKFWNTHTGACLNSVDTGSQVCSLLWSKNER 361

Query: 370 ELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNV 429
           ELLSSHGF+QNQLTLWKYPSMVKM ELTGHTSRVL+MAQSPDGCTVASAA DETLRFWNV
Sbjct: 362 ELLSSHGFTQNQLTLWKYPSMVKMAELTGHTSRVLYMAQSPDGCTVASAAGDETLRFWNV 421

Query: 430 FGAPEVAKPAAKSYNAEPFAHINRIR 454
           FG PE AK AA    AEPF+H+NRIR
Sbjct: 422 FGVPETAKKAAPKAVAEPFSHVNRIR 447

BLAST of Carg08474 vs. ExPASy Swiss-Prot
Match: Q3E906 (Cell division cycle 20.5, cofactor of APC complex OS=Arabidopsis thaliana OX=3702 GN=CDC20-5 PE=1 SV=3)

HSP 1 Score: 647.1 bits (1668), Expect = 1.5e-184
Identity = 316/455 (69.45%), Postives = 364/455 (80.00%), Query Frame = 0

Query: 7   SSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKENPSICSP 66
           SS    ++ CPL E  L++K S+EN DRFIPNRSAMDFD+A+Y +T GRK +    + S 
Sbjct: 5   SSHLKAQASCPLVEHFLRRKLSKENFDRFIPNRSAMDFDFANYALTQGRK-RNVDEVTSA 64

Query: 67  SREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKP------VKARRHI 126
           SR+AY  +LAE +N NRTRILAFRNK        PK   SS  +D P      VK RR+I
Sbjct: 65  SRKAYMTQLAEAMNQNRTRILAFRNK--------PKALLSSNHSDPPHQQPISVKPRRYI 124

Query: 127 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 186
           PQ SER LDAP + DD+YLNLLDWGS+NVLAIALG+TVYLW+A+ GST +LVT+D+E GP
Sbjct: 125 PQNSERVLDAPGIADDFYLNLLDWGSSNVLAIALGDTVYLWDASSGSTYKLVTIDEEEGP 184

Query: 187 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 246
           +TS+NW  DG  + IG  NSEVQLWD  +NRQ+RTLRGGH  RVGSLAWNNHILTTGGMD
Sbjct: 185 VTSINWTQDGLDLAIGLDNSEVQLWDCVSNRQVRTLRGGHESRVGSLAWNNHILTTGGMD 244

Query: 247 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWD-RSMASSNSA 306
           GKI+NNDVRIR  IVETY GH +EVCGLKWS SG++LASGGNDNV+HIWD RS+ASSN  
Sbjct: 245 GKIVNNDVRIRSSIVETYLGHTEEVCGLKWSESGKKLASGGNDNVVHIWDHRSVASSNPT 304

Query: 307 TQWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCA 366
            QWLHR E+HT+AV+ALAWCPFQ++LLATGGG  D  IKFWNTHTGACLNSV+TGSQVC+
Sbjct: 305 RQWLHRFEEHTAAVRALAWCPFQASLLATGGGVGDGKIKFWNTHTGACLNSVETGSQVCS 364

Query: 367 LLWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAAD 426
           LLW+K+ERELLSSHGF+QNQLTLWKYPSMVKM EL GHTSRVLFMAQSPDGCTVASAA D
Sbjct: 365 LLWSKSERELLSSHGFTQNQLTLWKYPSMVKMAELNGHTSRVLFMAQSPDGCTVASAAGD 424

Query: 427 ETLRFWNVFG-APEVAKPAAKSYNAEPFAHINRIR 454
           ETLR WNVFG  P+  K AA     +PFAH+N IR
Sbjct: 425 ETLRLWNVFGEPPKTTKKAASKKYTDPFAHVNHIR 450

BLAST of Carg08474 vs. ExPASy Swiss-Prot
Match: Q4PSE4 (Cell division cycle 20.4, cofactor of APC complex OS=Arabidopsis thaliana OX=3702 GN=CDC20-4 PE=2 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 3.5e-178
Identity = 301/442 (68.10%), Postives = 353/442 (79.86%), Query Frame = 0

Query: 13  RSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKENPSICSPSREAYQ 72
           R+ C + E  L +K S++NLDRFIPNRSA DFD+A+Y +T G K +    + S SR+AY 
Sbjct: 6   RATCTVPEHFLPRKLSKQNLDRFIPNRSAKDFDFANYALTQGSK-RNLDEVTSASRKAYM 65

Query: 73  KRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHIPQTSERTLDAPD 132
            +LA  +N NRTRILAFRNKP + +     +  S   N KPVK RR+IPQ SER LDAP 
Sbjct: 66  TQLAVVMNQNRTRILAFRNKPKSLLSTNHSD--SPHQNPKPVKPRRYIPQNSERVLDAPG 125

Query: 133 LVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWAPDGRH 192
           L DD+ LNLLDWGSANVLAIALG+TVYLW+A+ GSTSELVT+D++ GP+TS+NW  DG  
Sbjct: 126 LRDDFSLNLLDWGSANVLAIALGDTVYLWDASSGSTSELVTIDEDKGPVTSINWTQDGLD 185

Query: 193 IGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINNDVRIRD 252
           + +G  NSEVQLWD  +NRQ+RTLRGGH  RVGSLAW+NHILTTGGMDGKI+NNDVRIR 
Sbjct: 186 LAVGLDNSEVQLWDCVSNRQVRTLRGGHESRVGSLAWDNHILTTGGMDGKIVNNDVRIRS 245

Query: 253 HIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSATQWLHRLEDHTSA 312
            IVETY GH +EVCGLKWS SG + ASGGNDNV+HIWDRS+ASS    QWLHR E+HT+A
Sbjct: 246 SIVETYLGHTEEVCGLKWSESGNKQASGGNDNVVHIWDRSLASSKQTRQWLHRFEEHTAA 305

Query: 313 VKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNERELLSS 372
           V+ALAWCPFQ++LLATGGG  D  IKFWNTHTGACLNSV+TGSQVC+LLW+++ERELLSS
Sbjct: 306 VRALAWCPFQASLLATGGGVGDGKIKFWNTHTGACLNSVETGSQVCSLLWSQSERELLSS 365

Query: 373 HGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNVFG-AP 432
           HGF+QNQLTLWKYPSM KM EL GHTSRVLFMAQSP+GCTVASAA DE LR WNVFG  P
Sbjct: 366 HGFTQNQLTLWKYPSMSKMAELNGHTSRVLFMAQSPNGCTVASAAGDENLRLWNVFGEPP 425

Query: 433 EVAKPAAKSYNAEPFAHINRIR 454
           +  K AA     E F+H+N +R
Sbjct: 426 KTTKKAASKKYPELFSHVNSLR 444

BLAST of Carg08474 vs. ExPASy Swiss-Prot
Match: Q9S7H3 (Cell division cycle 20.3, cofactor of APC complex OS=Arabidopsis thaliana OX=3702 GN=CDC20-3 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 1.5e-176
Identity = 303/440 (68.86%), Postives = 350/440 (79.55%), Query Frame = 0

Query: 16  CPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKENPSICSPSREAYQKRL 75
           C + +  L +K S++NLDRFIPNRSAMDFD+A+Y +T GRK +    I S SR+AY  +L
Sbjct: 6   CTVPDHFLPRKLSKQNLDRFIPNRSAMDFDFANYALTQGRK-RNVDEITSASRKAYMTQL 65

Query: 76  AETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHIPQTSERTLDAPDLVD 135
           A  +N NRTRILAFRNKP A   L      S   N K VK RR+IPQ SER LDAP L+D
Sbjct: 66  AVVMNQNRTRILAFRNKPKA--LLSSNHSDSPHQNPKSVKPRRYIPQNSERVLDAPGLMD 125

Query: 136 DYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWAPDGRHIGI 195
           D+YLNLLDWGSANVLAIALG+TVYLW+A+ GSTSELVT+D++ GP+TS+NW  DG  + +
Sbjct: 126 DFYLNLLDWGSANVLAIALGDTVYLWDASSGSTSELVTIDEDKGPVTSINWTQDGLDLAV 185

Query: 196 GFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINNDVRIRDHIV 255
           G  NSEVQLWD  +NRQ+RTL GGH  RVGSLAWNNHILTTGGMDGKI+NNDVRIR  IV
Sbjct: 186 GLDNSEVQLWDFVSNRQVRTLIGGHESRVGSLAWNNHILTTGGMDGKIVNNDVRIRSSIV 245

Query: 256 ETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWD-RSMASSNSATQWLHRLEDHTSAVK 315
            TY GH +EVCGLKWS SG++LASGGN NV+HIWD RS+ASS    QWLHR E+HT+AV+
Sbjct: 246 GTYLGHTEEVCGLKWSESGKKLASGGNYNVVHIWDHRSVASSKPTRQWLHRFEEHTAAVR 305

Query: 316 ALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNERELLSSHG 375
           ALAWCPFQ+ LLATGGG  D  IKFWNTHTGACLNSV+TGSQVC+LLW++ ERELLSSHG
Sbjct: 306 ALAWCPFQATLLATGGGVGDGKIKFWNTHTGACLNSVETGSQVCSLLWSQRERELLSSHG 365

Query: 376 FSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNVFG-APEV 435
           F+QNQLTLWKYPSM KM EL GHTSRVLFMAQSP+GCTVASAA DE LR WNVFG  P+ 
Sbjct: 366 FTQNQLTLWKYPSMSKMAELNGHTSRVLFMAQSPNGCTVASAAGDENLRLWNVFGEPPKT 425

Query: 436 AKPAAKSYNAEPFAHINRIR 454
            K AA     E F+H+N +R
Sbjct: 426 TKKAASKNYLELFSHVNSLR 442

BLAST of Carg08474 vs. ExPASy TrEMBL
Match: A0A6J1JY99 (cell division cycle 20.2, cofactor of APC complex-like OS=Cucurbita maxima OX=3661 GN=LOC111490848 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 5.4e-267
Identity = 449/453 (99.12%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDA SLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN
Sbjct: 1   MDAASLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
            SICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI
Sbjct: 61  SSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           ITSVNWAPDGRHI IGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 ITSVNWAPDGRHIAIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKIINNDVRIRDHIV TYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKIINNDVRIRDHIVGTYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Sbjct: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 453

BLAST of Carg08474 vs. ExPASy TrEMBL
Match: A0A6J1H3W9 (cell division cycle 20.2, cofactor of APC complex-like OS=Cucurbita moschata OX=3662 GN=LOC111459868 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 9.2e-267
Identity = 449/453 (99.12%), Postives = 449/453 (99.12%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLSSSNAGRSRCPLQEQ LQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN
Sbjct: 1   MDAGSLSSSNAGRSRCPLQEQQLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI
Sbjct: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           ITSVNWAPDGRHIGIGF NSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 ITSVNWAPDGRHIGIGFQNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKIINNDVRIRDHIV TYRGHDQEVCGLKWS SGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKIINNDVRIRDHIVGTYRGHDQEVCGLKWSSSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR
Sbjct: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 453

BLAST of Carg08474 vs. ExPASy TrEMBL
Match: A0A0A0LCG1 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G702570 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.5e-253
Identity = 423/453 (93.38%), Postives = 436/453 (96.25%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLS+SNA  SRCPLQEQHLQ+K SRENLDRFIPNRSAMDFDYAHYMVT+GRKGKEN
Sbjct: 1   MDAGSLSTSNANHSRCPLQEQHLQRKQSRENLDRFIPNRSAMDFDYAHYMVTEGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PS  SPSREAYQKRLAET NMNRTRILAF+NKPPAPVELIPKEFFSS S+DKPVKARRHI
Sbjct: 61  PSFSSPSREAYQKRLAETFNMNRTRILAFKNKPPAPVELIPKEFFSSVSHDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSE+TLDAPDLVDDYYLNLLDWGS+NVLAIALGNTVYLWNA DGSTSELVTVDDEVGP
Sbjct: 121 PQTSEKTLDAPDLVDDYYLNLLDWGSSNVLAIALGNTVYLWNATDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           +TSV+WAPDGRHI IG +NSEVQLWDSTANRQLRTL+GGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 VTSVSWAPDGRHIAIGLNNSEVQLWDSTANRQLRTLKGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKI+NNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKILNNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLEDHTSAVKALAWCPFQ NLLATGGGA DRTIKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEDHTSAVKALAWCPFQGNLLATGGGAGDRTIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFG PEVAKPA KS   EPFAH+NRIR
Sbjct: 421 TLRFWNVFGTPEVAKPAPKSSVTEPFAHLNRIR 453

BLAST of Carg08474 vs. ExPASy TrEMBL
Match: A0A5D3CHD8 (Cell division cycle 20.2, cofactor of APC complex-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00100 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.4e-251
Identity = 420/453 (92.72%), Postives = 432/453 (95.36%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLS+SNA  SRCPLQEQHLQ+K SRENLDRFIPNRSAMDFDYAHYMVT+GRKGKEN
Sbjct: 1   MDAGSLSASNANHSRCPLQEQHLQRKQSRENLDRFIPNRSAMDFDYAHYMVTEGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PS  SPSREAYQKRLAET NMNRTRILAF+NKPPAPVELIPKEFFSS S+DKPVKARRHI
Sbjct: 61  PSFSSPSREAYQKRLAETFNMNRTRILAFKNKPPAPVELIPKEFFSSVSHDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGS NVLAIALGNTVYLWNA DGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSGNVLAIALGNTVYLWNATDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           +TSV+WAPDGRHI IG +NSE+QLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 VTSVSWAPDGRHIAIGLNNSEIQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKI+NNDVRIRDHIVE YRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKILNNDVRIRDHIVENYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLE+HTSAVKALAWCPFQ NLLATGGGA DR IKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEEHTSAVKALAWCPFQGNLLATGGGAGDRNIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGEL GHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELNGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFG PEVAKPA KS   EPFAH+NRIR
Sbjct: 421 TLRFWNVFGTPEVAKPAPKSSVTEPFAHLNRIR 453

BLAST of Carg08474 vs. ExPASy TrEMBL
Match: A0A1S3CFY1 (cell division cycle 20.2, cofactor of APC complex-like OS=Cucumis melo OX=3656 GN=LOC103500295 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.4e-251
Identity = 420/453 (92.72%), Postives = 432/453 (95.36%), Query Frame = 0

Query: 1   MDAGSLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKEN 60
           MDAGSLS+SNA  SRCPLQEQHLQ+K SRENLDRFIPNRSAMDFDYAHYMVT+GRKGKEN
Sbjct: 1   MDAGSLSASNANHSRCPLQEQHLQRKQSRENLDRFIPNRSAMDFDYAHYMVTEGRKGKEN 60

Query: 61  PSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHI 120
           PS  SPSREAYQKRLAET NMNRTRILAF+NKPPAPVELIPKEFFSS S+DKPVKARRHI
Sbjct: 61  PSFSSPSREAYQKRLAETFNMNRTRILAFKNKPPAPVELIPKEFFSSVSHDKPVKARRHI 120

Query: 121 PQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGP 180
           PQTSERTLDAPDLVDDYYLNLLDWGS NVLAIALGNTVYLWNA DGSTSELVTVDDEVGP
Sbjct: 121 PQTSERTLDAPDLVDDYYLNLLDWGSGNVLAIALGNTVYLWNATDGSTSELVTVDDEVGP 180

Query: 181 ITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240
           +TSV+WAPDGRHI IG +NSE+QLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD
Sbjct: 181 VTSVSWAPDGRHIAIGLNNSEIQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMD 240

Query: 241 GKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300
           GKI+NNDVRIRDHIVE YRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT
Sbjct: 241 GKILNNDVRIRDHIVENYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSAT 300

Query: 301 QWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCAL 360
           QWLHRLE+HTSAVKALAWCPFQ NLLATGGGA DR IKFWNTHTGACLNSVDTGSQVCAL
Sbjct: 301 QWLHRLEEHTSAVKALAWCPFQGNLLATGGGAGDRNIKFWNTHTGACLNSVDTGSQVCAL 360

Query: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADE 420
           LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGEL GHTSRVLFMAQSPDGCTVASAAADE
Sbjct: 361 LWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELNGHTSRVLFMAQSPDGCTVASAAADE 420

Query: 421 TLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           TLRFWNVFG PEVAKPA KS   EPFAH+NRIR
Sbjct: 421 TLRFWNVFGTPEVAKPAPKSSVTEPFAHLNRIR 453

BLAST of Carg08474 vs. TAIR 10
Match: AT4G33270.1 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 747.7 bits (1929), Expect = 5.6e-216
Identity = 358/457 (78.34%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MDAG--SLSSSNAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGK 60
           MDAG  + SS    ++RCPLQE  L +K S+ENLDRFIPNRSAM+FDYAH+ +T+GRKGK
Sbjct: 1   MDAGMNNTSSHYKTQARCPLQEHFLPRKPSKENLDRFIPNRSAMNFDYAHFALTEGRKGK 60

Query: 61  -ENPSICSPSREAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSS-GSNDKPVKA 120
            +  ++ SPS+EAY+K+LAET+N+N TRILAFRNKP APVEL+P    +S     K VK 
Sbjct: 61  DQTAAVSSPSKEAYRKQLAETMNLNHTRILAFRNKPQAPVELLPSNHSASLHQQPKSVKP 120

Query: 121 RRHIPQTSERTLDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDD 180
           RR+IPQTSERTLDAPD+VDD+YLNLLDWGSANVLAIAL +TVYLW+A+ GSTSELVT+D+
Sbjct: 121 RRYIPQTSERTLDAPDIVDDFYLNLLDWGSANVLAIALDHTVYLWDASTGSTSELVTIDE 180

Query: 181 EVGPITSVNWAPDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTT 240
           E GP+TS+NWAPDGRH+ +G +NSEVQLWDS +NRQLRTL+GGH+ RVGSLAWNNHILTT
Sbjct: 181 EKGPVTSINWAPDGRHVAVGLNNSEVQLWDSASNRQLRTLKGGHQSRVGSLAWNNHILTT 240

Query: 241 GGMDGKIINNDVRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASS 300
           GGMDG IINNDVRIR  IVETYRGH QEVCGLKWS SGQQLASGGNDNV+HIWDRS+ASS
Sbjct: 241 GGMDGLIINNDVRIRSPIVETYRGHTQEVCGLKWSGSGQQLASGGNDNVVHIWDRSVASS 300

Query: 301 NSATQWLHRLEDHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQ 360
           NS TQWLHRLE+HTSAVKALAWCPFQ+NLLATGGG  DRTIKFWNTHTGACLNSVDTGSQ
Sbjct: 301 NSTTQWLHRLEEHTSAVKALAWCPFQANLLATGGGGGDRTIKFWNTHTGACLNSVDTGSQ 360

Query: 361 VCALLWNKNERELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASA 420
           VC+LLW+KNERELLSSHGF+QNQLTLWKYPSMVKM ELTGHTSRVL+MAQSPDGCTVASA
Sbjct: 361 VCSLLWSKNERELLSSHGFTQNQLTLWKYPSMVKMAELTGHTSRVLYMAQSPDGCTVASA 420

Query: 421 AADETLRFWNVFGAPEVAKPAAKSYNAEPFAHINRIR 454
           A DETLRFWNVFG PE AK AA    +EPF+H+NRIR
Sbjct: 421 AGDETLRFWNVFGVPETAKKAAPKAVSEPFSHVNRIR 457

BLAST of Carg08474 vs. TAIR 10
Match: AT4G33260.1 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 746.9 bits (1927), Expect = 9.6e-216
Identity = 354/446 (79.37%), Postives = 400/446 (89.69%), Query Frame = 0

Query: 10  NAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGK-ENPSICSPSR 69
           +AG +RCPLQE  L +K+S+ENLDRFIPNRSAM+FDYAH+ +T+ RKGK ++ ++ SPS+
Sbjct: 2   DAGLNRCPLQEHFLPRKNSKENLDRFIPNRSAMNFDYAHFALTEERKGKDQSATVSSPSK 61

Query: 70  EAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSS-GSNDKPVKARRHIPQTSERT 129
           EAY+K+LAET+N+N TRILAFRNKP APVEL+P    +S     K VK RR+IPQTSERT
Sbjct: 62  EAYRKQLAETMNLNHTRILAFRNKPQAPVELLPSNHSASLHQQPKSVKPRRYIPQTSERT 121

Query: 130 LDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWA 189
           LDAPD+VDD+YLNLLDWGSANVLAIAL +TVYLW+A+ GSTSELVT+D+E GP+TS+NWA
Sbjct: 122 LDAPDIVDDFYLNLLDWGSANVLAIALDHTVYLWDASTGSTSELVTIDEEKGPVTSINWA 181

Query: 190 PDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINND 249
           PDGRH+ +G +NSEVQLWDS +NRQLRTL+GGH+ RVGSLAWNNHILTTGGMDG IINND
Sbjct: 182 PDGRHVAVGLNNSEVQLWDSASNRQLRTLKGGHQSRVGSLAWNNHILTTGGMDGLIINND 241

Query: 250 VRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSATQWLHRLE 309
           VRIR  IVETYRGH QEVCGLKWS SGQQLASGGNDNV+HIWDRS+ASSNS TQWLHRLE
Sbjct: 242 VRIRSPIVETYRGHTQEVCGLKWSGSGQQLASGGNDNVVHIWDRSVASSNSTTQWLHRLE 301

Query: 310 DHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNER 369
           +HTSAVKALAWCPFQ+NLLATGGG  DRTIKFWNTHTGACLNSVDTGSQVC+LLW+KNER
Sbjct: 302 EHTSAVKALAWCPFQANLLATGGGGGDRTIKFWNTHTGACLNSVDTGSQVCSLLWSKNER 361

Query: 370 ELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNV 429
           ELLSSHGF+QNQLTLWKYPSMVKM ELTGHTSRVL+MAQSPDGCTVASAA DETLRFWNV
Sbjct: 362 ELLSSHGFTQNQLTLWKYPSMVKMAELTGHTSRVLYMAQSPDGCTVASAAGDETLRFWNV 421

Query: 430 FGAPEVAKPAAKSYNAEPFAHINRIR 454
           FG PE AK AA    AEPF+H+NRIR
Sbjct: 422 FGVPETAKKAAPKAVAEPFSHVNRIR 447

BLAST of Carg08474 vs. TAIR 10
Match: AT4G33260.2 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 701.0 bits (1808), Expect = 6.0e-202
Identity = 334/433 (77.14%), Postives = 381/433 (87.99%), Query Frame = 0

Query: 10  NAGRSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGK-ENPSICSPSR 69
           +AG +RCPLQE  L +K+S+ENLDRFIPNRSAM+FDYAH+ +T+ RKGK ++ ++ SPS+
Sbjct: 2   DAGLNRCPLQEHFLPRKNSKENLDRFIPNRSAMNFDYAHFALTEERKGKDQSATVSSPSK 61

Query: 70  EAYQKRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSS-GSNDKPVKARRHIPQTSERT 129
           EAY+K+LAET+N+N TRILAFRNKP APVEL+P    +S     K VK RR+IPQTSERT
Sbjct: 62  EAYRKQLAETMNLNHTRILAFRNKPQAPVELLPSNHSASLHQQPKSVKPRRYIPQTSERT 121

Query: 130 LDAPDLVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWA 189
           LDAPD+VDD+YLNLLDWGSANVLAIAL +TVYLW+A+ GSTSELVT+D+E GP+TS+NWA
Sbjct: 122 LDAPDIVDDFYLNLLDWGSANVLAIALDHTVYLWDASTGSTSELVTIDEEKGPVTSINWA 181

Query: 190 PDGRHIGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINND 249
           PDGRH+ +G +NSEVQLWDS +NRQLRTL+GGH+ RVGSLAWNNHILTTGGMDG IINND
Sbjct: 182 PDGRHVAVGLNNSEVQLWDSASNRQLRTLKGGHQSRVGSLAWNNHILTTGGMDGLIINND 241

Query: 250 VRIRDHIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSATQWLHRLE 309
           VRIR  IVETYRGH QEVCGLKWS SGQQLASGGNDNV+HIWDRS+ASSNS TQWLHRLE
Sbjct: 242 VRIRSPIVETYRGHTQEVCGLKWSGSGQQLASGGNDNVVHIWDRSVASSNSTTQWLHRLE 301

Query: 310 DHTSAVKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNER 369
           +HTSAVKALAWCPFQ+NLLATGGG  DRTIKFWNTHTGACLNSVDTGSQVC+LLW+KNER
Sbjct: 302 EHTSAVKALAWCPFQANLLATGGGGGDRTIKFWNTHTGACLNSVDTGSQVCSLLWSKNER 361

Query: 370 ELLSSHGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNV 429
           ELLSSHGF+QNQLTLWKYPSMVKM ELTGHTSRVL+MAQSPDGCTVASAA DETL    +
Sbjct: 362 ELLSSHGFTQNQLTLWKYPSMVKMAELTGHTSRVLYMAQSPDGCTVASAAGDETLSCRAI 421

Query: 430 FGAPEVAKPAAKS 441
           F     +    K+
Sbjct: 422 FSCESYSMKLLKT 434

BLAST of Carg08474 vs. TAIR 10
Match: AT5G26900.1 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 625.9 bits (1613), Expect = 2.5e-179
Identity = 301/442 (68.10%), Postives = 353/442 (79.86%), Query Frame = 0

Query: 13  RSRCPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKENPSICSPSREAYQ 72
           R+ C + E  L +K S++NLDRFIPNRSA DFD+A+Y +T G K +    + S SR+AY 
Sbjct: 6   RATCTVPEHFLPRKLSKQNLDRFIPNRSAKDFDFANYALTQGSK-RNLDEVTSASRKAYM 65

Query: 73  KRLAETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHIPQTSERTLDAPD 132
            +LA  +N NRTRILAFRNKP + +     +  S   N KPVK RR+IPQ SER LDAP 
Sbjct: 66  TQLAVVMNQNRTRILAFRNKPKSLLSTNHSD--SPHQNPKPVKPRRYIPQNSERVLDAPG 125

Query: 133 LVDDYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWAPDGRH 192
           L DD+ LNLLDWGSANVLAIALG+TVYLW+A+ GSTSELVT+D++ GP+TS+NW  DG  
Sbjct: 126 LRDDFSLNLLDWGSANVLAIALGDTVYLWDASSGSTSELVTIDEDKGPVTSINWTQDGLD 185

Query: 193 IGIGFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINNDVRIRD 252
           + +G  NSEVQLWD  +NRQ+RTLRGGH  RVGSLAW+NHILTTGGMDGKI+NNDVRIR 
Sbjct: 186 LAVGLDNSEVQLWDCVSNRQVRTLRGGHESRVGSLAWDNHILTTGGMDGKIVNNDVRIRS 245

Query: 253 HIVETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWDRSMASSNSATQWLHRLEDHTSA 312
            IVETY GH +EVCGLKWS SG + ASGGNDNV+HIWDRS+ASS    QWLHR E+HT+A
Sbjct: 246 SIVETYLGHTEEVCGLKWSESGNKQASGGNDNVVHIWDRSLASSKQTRQWLHRFEEHTAA 305

Query: 313 VKALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNERELLSS 372
           V+ALAWCPFQ++LLATGGG  D  IKFWNTHTGACLNSV+TGSQVC+LLW+++ERELLSS
Sbjct: 306 VRALAWCPFQASLLATGGGVGDGKIKFWNTHTGACLNSVETGSQVCSLLWSQSERELLSS 365

Query: 373 HGFSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNVFG-AP 432
           HGF+QNQLTLWKYPSM KM EL GHTSRVLFMAQSP+GCTVASAA DE LR WNVFG  P
Sbjct: 366 HGFTQNQLTLWKYPSMSKMAELNGHTSRVLFMAQSPNGCTVASAAGDENLRLWNVFGEPP 425

Query: 433 EVAKPAAKSYNAEPFAHINRIR 454
           +  K AA     E F+H+N +R
Sbjct: 426 KTTKKAASKKYPELFSHVNSLR 444

BLAST of Carg08474 vs. TAIR 10
Match: AT5G27080.1 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 620.5 bits (1599), Expect = 1.0e-177
Identity = 303/440 (68.86%), Postives = 350/440 (79.55%), Query Frame = 0

Query: 16  CPLQEQHLQKKHSRENLDRFIPNRSAMDFDYAHYMVTDGRKGKENPSICSPSREAYQKRL 75
           C + +  L +K S++NLDRFIPNRSAMDFD+A+Y +T GRK +    I S SR+AY  +L
Sbjct: 6   CTVPDHFLPRKLSKQNLDRFIPNRSAMDFDFANYALTQGRK-RNVDEITSASRKAYMTQL 65

Query: 76  AETLNMNRTRILAFRNKPPAPVELIPKEFFSSGSNDKPVKARRHIPQTSERTLDAPDLVD 135
           A  +N NRTRILAFRNKP A   L      S   N K VK RR+IPQ SER LDAP L+D
Sbjct: 66  AVVMNQNRTRILAFRNKPKA--LLSSNHSDSPHQNPKSVKPRRYIPQNSERVLDAPGLMD 125

Query: 136 DYYLNLLDWGSANVLAIALGNTVYLWNANDGSTSELVTVDDEVGPITSVNWAPDGRHIGI 195
           D+YLNLLDWGSANVLAIALG+TVYLW+A+ GSTSELVT+D++ GP+TS+NW  DG  + +
Sbjct: 126 DFYLNLLDWGSANVLAIALGDTVYLWDASSGSTSELVTIDEDKGPVTSINWTQDGLDLAV 185

Query: 196 GFHNSEVQLWDSTANRQLRTLRGGHRMRVGSLAWNNHILTTGGMDGKIINNDVRIRDHIV 255
           G  NSEVQLWD  +NRQ+RTL GGH  RVGSLAWNNHILTTGGMDGKI+NNDVRIR  IV
Sbjct: 186 GLDNSEVQLWDFVSNRQVRTLIGGHESRVGSLAWNNHILTTGGMDGKIVNNDVRIRSSIV 245

Query: 256 ETYRGHDQEVCGLKWSLSGQQLASGGNDNVLHIWD-RSMASSNSATQWLHRLEDHTSAVK 315
            TY GH +EVCGLKWS SG++LASGGN NV+HIWD RS+ASS    QWLHR E+HT+AV+
Sbjct: 246 GTYLGHTEEVCGLKWSESGKKLASGGNYNVVHIWDHRSVASSKPTRQWLHRFEEHTAAVR 305

Query: 316 ALAWCPFQSNLLATGGGASDRTIKFWNTHTGACLNSVDTGSQVCALLWNKNERELLSSHG 375
           ALAWCPFQ+ LLATGGG  D  IKFWNTHTGACLNSV+TGSQVC+LLW++ ERELLSSHG
Sbjct: 306 ALAWCPFQATLLATGGGVGDGKIKFWNTHTGACLNSVETGSQVCSLLWSQRERELLSSHG 365

Query: 376 FSQNQLTLWKYPSMVKMGELTGHTSRVLFMAQSPDGCTVASAAADETLRFWNVFG-APEV 435
           F+QNQLTLWKYPSM KM EL GHTSRVLFMAQSP+GCTVASAA DE LR WNVFG  P+ 
Sbjct: 366 FTQNQLTLWKYPSMSKMAELNGHTSRVLFMAQSPNGCTVASAAGDENLRLWNVFGEPPKT 425

Query: 436 AKPAAKSYNAEPFAHINRIR 454
            K AA     E F+H+N +R
Sbjct: 426 TKKAASKNYLELFSHVNSLR 442

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035573.18.3e-270100.00Cell division cycle 20.1, cofactor of APC complex [Cucurbita argyrosperma subsp.... [more]
XP_023532453.12.7e-26899.56cell division cycle 20.2, cofactor of APC complex-like [Cucurbita pepo subsp. pe... [more]
XP_022995242.11.1e-26699.12cell division cycle 20.2, cofactor of APC complex-like [Cucurbita maxima][more]
XP_022958728.11.9e-26699.12cell division cycle 20.2, cofactor of APC complex-like [Cucurbita moschata][more]
KAG6605663.11.5e-25596.69Cell division cycle 20.1, cofactor of APC complex, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
Q9SZA47.9e-21578.34Cell division cycle 20.1, cofactor of APC complex OS=Arabidopsis thaliana OX=370... [more]
Q9S7I81.3e-21479.37Cell division cycle 20.2, cofactor of APC complex OS=Arabidopsis thaliana OX=370... [more]
Q3E9061.5e-18469.45Cell division cycle 20.5, cofactor of APC complex OS=Arabidopsis thaliana OX=370... [more]
Q4PSE43.5e-17868.10Cell division cycle 20.4, cofactor of APC complex OS=Arabidopsis thaliana OX=370... [more]
Q9S7H31.5e-17668.86Cell division cycle 20.3, cofactor of APC complex OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1JY995.4e-26799.12cell division cycle 20.2, cofactor of APC complex-like OS=Cucurbita maxima OX=36... [more]
A0A6J1H3W99.2e-26799.12cell division cycle 20.2, cofactor of APC complex-like OS=Cucurbita moschata OX=... [more]
A0A0A0LCG14.5e-25393.38WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A5D3CHD82.4e-25192.72Cell division cycle 20.2, cofactor of APC complex-like OS=Cucumis melo var. maku... [more]
A0A1S3CFY12.4e-25192.72cell division cycle 20.2, cofactor of APC complex-like OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT4G33270.15.6e-21678.34Transducin family protein / WD-40 repeat family protein [more]
AT4G33260.19.6e-21679.37Transducin family protein / WD-40 repeat family protein [more]
AT4G33260.26.0e-20277.14Transducin family protein / WD-40 repeat family protein [more]
AT5G26900.12.5e-17968.10Transducin family protein / WD-40 repeat family protein [more]
AT5G27080.11.0e-17768.86Transducin family protein / WD-40 repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 209..247
e-value: 1.9
score: 15.2
coord: 344..384
e-value: 360.0
score: 0.9
coord: 251..290
e-value: 4.7E-8
score: 42.8
coord: 299..341
e-value: 8.8E-5
score: 31.9
coord: 167..206
e-value: 0.066
score: 22.3
coord: 387..426
e-value: 1.4E-5
score: 34.5
IPR001680WD40 repeatPFAMPF00400WD40coord: 391..426
e-value: 4.9E-4
score: 20.8
coord: 303..341
e-value: 5.6E-4
score: 20.7
coord: 256..290
e-value: 3.8E-6
score: 27.5
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 306..350
score: 8.67045
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 258..290
score: 13.516088
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 174..215
score: 10.107432
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 394..427
score: 12.714052
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 82..429
e-value: 1.4E-122
score: 410.9
IPR024977Anaphase-promoting complex subunit 4, WD40 domainPFAMPF12894ANAPC4_WD40coord: 173..231
e-value: 1.6E-4
score: 21.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availablePANTHERPTHR19918:SF50CELL DIVISION CYCLE 20.2, COFACTOR OF APC COMPLEX-LIKEcoord: 1..451
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 258..290
score: 11.207411
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 394..427
score: 10.574686
NoneNo IPR availableCDDcd00200WD40coord: 156..426
e-value: 1.38413E-39
score: 142.089
IPR033010The WD repeat Cdc20/Fizzy familyPANTHERPTHR19918CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATEDcoord: 1..451
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 413..427
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 328..342
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 138..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08474-RACarg08474-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:1905786 positive regulation of anaphase-promoting complex-dependent catabolic process
biological_process GO:1904668 positive regulation of ubiquitin protein ligase activity
cellular_component GO:0005680 anaphase-promoting complex
molecular_function GO:0010997 anaphase-promoting complex binding
molecular_function GO:0005515 protein binding
molecular_function GO:1990757 ubiquitin ligase activator activity
molecular_function GO:0097027 ubiquitin-protein transferase activator activity